S. N. Sibikeev, I. G. Adonina, A. E. Druzhin, O. A. Baranova
Triticum timopheevii Zhuk. attracts the attention of bread wheat breeders with its high immunity to the leaf rust pathogen. However, introgressions from this species in Triticum aestivum L. are little used in practical breeding. In the presented study, the agronomic value of T. aestivum/T. timopheevii line L624 was studied in comparison with the parent cultivars Saratovskaya 68, Dobrynya and the standard cultivar Favorit during 2017–2022. Introgressions from T. timopheevii in L624 were detected by the FISH method with probes pSc119.2, pAs1 and Spelt1, as well as micro satellite markers Xgwm312, Xgpw4480 and Xksum73. Translocations of 2AS.2AL-2A t L and on 2DL were detected as well. Line L624 is highly resistant to Puccinia triticina both under the background of natural epiphytotics and under laboratory conditions. PCR analysis with the DNA marker of the LrTt1 gene (Xgwm312) revealed that it is not identical to the Lr gene(s) in L624. According to a five-year study, the grain yield of L624 was, on average, higher than that of Favorit and Dobrynya, but lower than that of Saratovskaya 68. Line L624 had a lower weight of 1000 grains than the recipients, and was at the same level with the standard cultivar Favorit. Introgressions from T. timopheevii in L624 increased the grain protein content by comparison with Saratovskaya 68 and Favorit, but it was at the same level as in Dobrynya. As for parameters of flour and bread, L624 was not inferior to the recipient cultivars, but by volume and porosity of bread, it surpassed Saratovskaya 68. Moreover, L624 surpassed Favorit by the elasticity of the dough, the ratio of the elas ticity of the dough to the extensibility and the strength of the flour. Thus, the results obtained suggest that introgressions in chromosomes 2A and 2D in L624 do not impair baking properties.
小麦timopheevvii Zhuk。对叶锈病具有较高的免疫力，引起了面包小麦育种家的重视。然而，该物种在小麦中的基因渗入很少用于实际育种。在本研究中，研究了稻瘟病菌/稻瘟病菌的农艺价值。2017-2022年，对timopheevvii系L624与亲本品种Saratovskaya 68、Dobrynya和标准品种Favorit进行了比较研究。采用FISH方法，利用探针pSc119.2、pAs1和Spelt1，以及微卫星标记Xgwm312、Xgpw4480和Xksum73检测了timopheevi在L624中的基因渗入。2AS易位。同时检测到2AL-2A t L和on 2DL。L624系在天然附生背景和实验室条件下都对小麦锈菌具有高度抗性。用LrTt1基因(Xgwm312)的DNA标记进行PCR分析，发现该基因与L624的Lr基因(s)不相同。根据一项为期5年的研究，L624的粮食产量平均高于Favorit和Dobrynya，但低于Saratovskaya 68。品系L624的千粒重低于受体，与标准品种Favorit处于同一水平。L624与Saratovskaya 68和Favorit相比，timopheevi基因的渗入提高了籽粒蛋白质含量，但与Dobrynya的水平相同。在面粉和面包的指标上，L624不逊于受体品种，但在面包的体积和孔隙度上，L624超过了萨拉托夫斯卡亚68。此外，L624在面团的弹性、面团的弹性与面粉的延伸性和强度之比上都超过了Favorit。因此，结果表明，L624的2A和2D染色体的渗入不会影响烘焙性能。
{"title":"Prebreeding studies of leaf rust resistant <i>Triticum aestivum/T. timopheevii</i> line L624","authors":"S. N. Sibikeev, I. G. Adonina, A. E. Druzhin, O. A. Baranova","doi":"10.18699/vjgb-23-73","DOIUrl":"https://doi.org/10.18699/vjgb-23-73","url":null,"abstract":"Triticum timopheevii Zhuk. attracts the attention of bread wheat breeders with its high immunity to the leaf rust pathogen. However, introgressions from this species in Triticum aestivum L. are little used in practical breeding. In the presented study, the agronomic value of T. aestivum/T. timopheevii line L624 was studied in comparison with the parent cultivars Saratovskaya 68, Dobrynya and the standard cultivar Favorit during 2017–2022. Introgressions from T. timopheevii in L624 were detected by the FISH method with probes pSc119.2, pAs1 and Spelt1, as well as micro satellite markers Xgwm312, Xgpw4480 and Xksum73. Translocations of 2AS.2AL-2A t L and on 2DL were detected as well. Line L624 is highly resistant to Puccinia triticina both under the background of natural epiphytotics and under laboratory conditions. PCR analysis with the DNA marker of the LrTt1 gene (Xgwm312) revealed that it is not identical to the Lr gene(s) in L624. According to a five-year study, the grain yield of L624 was, on average, higher than that of Favorit and Dobrynya, but lower than that of Saratovskaya 68. Line L624 had a lower weight of 1000 grains than the recipients, and was at the same level with the standard cultivar Favorit. Introgressions from T. timopheevii in L624 increased the grain protein content by comparison with Saratovskaya 68 and Favorit, but it was at the same level as in Dobrynya. As for parameters of flour and bread, L624 was not inferior to the recipient cultivars, but by volume and porosity of bread, it surpassed Saratovskaya 68. Moreover, L624 surpassed Favorit by the elasticity of the dough, the ratio of the elas ticity of the dough to the extensibility and the strength of the flour. Thus, the results obtained suggest that introgressions in chromosomes 2A and 2D in L624 do not impair baking properties.","PeriodicalId":486119,"journal":{"name":"Вавиловский журнал генетики и селекции","volume":"2 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135564920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. N. Shamshin, M. L. Dubrovsky, A. A. Trifonova, K. V. Boris, A. M. Kudryavtsev
Apple clonal rootstocks are the basis of modern intensive horticulture, providing a rapid increase in yield and convenience of fruit trees cultivation. Production of clonal rootstocks under high humidity often causes powdery mildew infection caused by the pathogenic fungus Podosphaera leucotricha Salm., which significantly reduces the productivity of stoolbed. Growing powdery mildew resistant genotypes is the most appropriate way to combat this disease and allows reducing the use of fungicides. To accelerate the search for resistant forms, molecular markers associated with resistance genes have been developed. However, these markers have not been used to study clonal rootstocks. The aims of the work were the field assessment of powdery mildew resistance of apple clonal rootstocks from the collection of the Michurinsk State Agrarian University and the screening of the collection for Pl-1, Pl-2, Pl-w and Pl-d resistance genes. The results of a three-year field evaluation of powdery mildew resistance of 80 rootstocks allowed us to distinguish five main groups ranging from very low to highly resistant. A group of 57 accessions was classified as powdery mildew resistant. The search for resistance genes was performed using the AT20 SCAR (Pl-1 gene), OPU02 SCAR (Pl- 2 gene), EM DM01 (Pl-d gene), and EM M02 (Pl-w gene) markers. The Pl-d and Pl-1 genes identified in 33 (41.25 %) and 31 (38.75 %) accessions, respectively, were the most common in the collection. The Pl-w gene was detected only in two accessions. Identification of the Pl-2 gene with the OPU02 SCAR marker did not reveal a fragment of the expected size. Thirty accessions with different powdery mildew resistance scores had two genes, Pl-1 and Pl-d, and highly resistant forms G16 and 14-1 had a combination of the Pl-d and Pl-w genes. These accessions can be used as donors of powdery mildew resistance for breeding new apple clonal rootstocks.
{"title":"Powdery mildew resistance of apple clonal rootstocks from the collection of the Michurinsk State Agrarian University","authors":"I. N. Shamshin, M. L. Dubrovsky, A. A. Trifonova, K. V. Boris, A. M. Kudryavtsev","doi":"10.18699/vjgb-23-69","DOIUrl":"https://doi.org/10.18699/vjgb-23-69","url":null,"abstract":"Apple clonal rootstocks are the basis of modern intensive horticulture, providing a rapid increase in yield and convenience of fruit trees cultivation. Production of clonal rootstocks under high humidity often causes powdery mildew infection caused by the pathogenic fungus Podosphaera leucotricha Salm., which significantly reduces the productivity of stoolbed. Growing powdery mildew resistant genotypes is the most appropriate way to combat this disease and allows reducing the use of fungicides. To accelerate the search for resistant forms, molecular markers associated with resistance genes have been developed. However, these markers have not been used to study clonal rootstocks. The aims of the work were the field assessment of powdery mildew resistance of apple clonal rootstocks from the collection of the Michurinsk State Agrarian University and the screening of the collection for Pl-1, Pl-2, Pl-w and Pl-d resistance genes. The results of a three-year field evaluation of powdery mildew resistance of 80 rootstocks allowed us to distinguish five main groups ranging from very low to highly resistant. A group of 57 accessions was classified as powdery mildew resistant. The search for resistance genes was performed using the AT20 SCAR (Pl-1 gene), OPU02 SCAR (Pl- 2 gene), EM DM01 (Pl-d gene), and EM M02 (Pl-w gene) markers. The Pl-d and Pl-1 genes identified in 33 (41.25 %) and 31 (38.75 %) accessions, respectively, were the most common in the collection. The Pl-w gene was detected only in two accessions. Identification of the Pl-2 gene with the OPU02 SCAR marker did not reveal a fragment of the expected size. Thirty accessions with different powdery mildew resistance scores had two genes, Pl-1 and Pl-d, and highly resistant forms G16 and 14-1 had a combination of the Pl-d and Pl-w genes. These accessions can be used as donors of powdery mildew resistance for breeding new apple clonal rootstocks.","PeriodicalId":486119,"journal":{"name":"Вавиловский журнал генетики и селекции","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135564253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Septoria is one of the harmful diseases of wheat cultivars cultivated in the Saratov region. This infectious disease of fungal etiology limits yield indicators and rapidly progresses in many regions of the Russian Federation. The aim of the research was to assess the resistance of winter and spring wheat cultivars that are referred to as promising and recommended for cultivation in the Low Volga region of the Russian Federation to pathogens of Septoria, to study the populations of Parastagonospora nodorum and P. pseudonodorum in the territory of the Saratov region in order to detect the presence of effector genes. Using molecular markers, we performed the identification of genes encoding NEs in 220 Parastagonospora spp. fungal isolates obtained from 7 cultivars of soft winter wheat, 6 taken from the winter triticale, 5 from soft spring wheat, 3 from durum spring wheat and 1 from spring oats. Among the P. nodorum isolates studied, there were both single genes Tox1, Tox3, and ToxA, and combinations of two genes in one genotype. The presence of the ToxA gene was not noted in the genotype of P. pseudonodorum isolates. During 2020–2022, a collection of winter and spring wheat cultivars was studied to detect resistance to Septoria blotch in field conditions (13 cultivars of winter wheat and 7 cultivars of spring wheat accordingly). The resistance of the cultivars was proven by laboratory evaluation. Three inoculums were used, including the isolates of Z. tritici, P. nodorum (ToxA, Tox1, Tox3), P. pseudonodorum (ToxA, Tox1, Tox3) mainly obtained from Saratov populations of 2022 (except for P. pseudonodorum with the ToxA gene). The tested cultivars were characterized using the Xfcp623 molecular marker, diagnostic for Tsn1/ tsn1 genes, which controls sensitivity to the fungal toxin of PtrToxA. Of greatest interest are 11 wheat genotypes that showed resistance to one, two and three species which served as causative agents of Septoria blotch (Zymoseptoria tritici, P. nodorum, P. pseudonodorum). These are the soft winter wheat cultivars Gostianum 237 (tsn1), Lutescens 230 (Tsn1), Guberniya (Tsn1), Podruga (Tsn1), Anastasia (Tsn1), Sosedka (Tsn1) and the soft spring wheat cultivars Favorit (tsn1), Prokhorovka (tsn1), Saratovskaya 70 (tsn1), Saratovskaya 73 (tsn1), Belyanka (tsn1). The results obtained are of interest as they might increase the efficiency of selection based on the elimination of genotypes with dominant Tsn1 alleles sensitive to PtrToxA. In addition to the economic value of the cultivars studied, it is recommended to use them in breeding for resistance to Septoria blotch.
{"title":"Soft wheat cultivars grown in the Saratov region and their resistance to Septoria blotch","authors":"Yu. V. Zeleneva, E. A. Konkova","doi":"10.18699/vjgb-23-70","DOIUrl":"https://doi.org/10.18699/vjgb-23-70","url":null,"abstract":"Septoria is one of the harmful diseases of wheat cultivars cultivated in the Saratov region. This infectious disease of fungal etiology limits yield indicators and rapidly progresses in many regions of the Russian Federation. The aim of the research was to assess the resistance of winter and spring wheat cultivars that are referred to as promising and recommended for cultivation in the Low Volga region of the Russian Federation to pathogens of Septoria, to study the populations of Parastagonospora nodorum and P. pseudonodorum in the territory of the Saratov region in order to detect the presence of effector genes. Using molecular markers, we performed the identification of genes encoding NEs in 220 Parastagonospora spp. fungal isolates obtained from 7 cultivars of soft winter wheat, 6 taken from the winter triticale, 5 from soft spring wheat, 3 from durum spring wheat and 1 from spring oats. Among the P. nodorum isolates studied, there were both single genes Tox1, Tox3, and ToxA, and combinations of two genes in one genotype. The presence of the ToxA gene was not noted in the genotype of P. pseudonodorum isolates. During 2020–2022, a collection of winter and spring wheat cultivars was studied to detect resistance to Septoria blotch in field conditions (13 cultivars of winter wheat and 7 cultivars of spring wheat accordingly). The resistance of the cultivars was proven by laboratory evaluation. Three inoculums were used, including the isolates of Z. tritici, P. nodorum (ToxA, Tox1, Tox3), P. pseudonodorum (ToxA, Tox1, Tox3) mainly obtained from Saratov populations of 2022 (except for P. pseudonodorum with the ToxA gene). The tested cultivars were characterized using the Xfcp623 molecular marker, diagnostic for Tsn1/ tsn1 genes, which controls sensitivity to the fungal toxin of PtrToxA. Of greatest interest are 11 wheat genotypes that showed resistance to one, two and three species which served as causative agents of Septoria blotch (Zymoseptoria tritici, P. nodorum, P. pseudonodorum). These are the soft winter wheat cultivars Gostianum 237 (tsn1), Lutescens 230 (Tsn1), Guberniya (Tsn1), Podruga (Tsn1), Anastasia (Tsn1), Sosedka (Tsn1) and the soft spring wheat cultivars Favorit (tsn1), Prokhorovka (tsn1), Saratovskaya 70 (tsn1), Saratovskaya 73 (tsn1), Belyanka (tsn1). The results obtained are of interest as they might increase the efficiency of selection based on the elimination of genotypes with dominant Tsn1 alleles sensitive to PtrToxA. In addition to the economic value of the cultivars studied, it is recommended to use them in breeding for resistance to Septoria blotch.","PeriodicalId":486119,"journal":{"name":"Вавиловский журнал генетики и селекции","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135565126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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