Journal of Periodontal and Implant Science最新文献

Purpose: The Korean Oral Biobank Network (KOBN) collects, stores, and provides oral samples for research. Quality control (QC) of biospecimens is necessary to ensure that they meet the basic prerequisites before being sent to researchers. This study presents the standard operating procedures (SOPs) for the QC of biospecimens.

Methods: QC methods using molecular genetic techniques according to sample types, including teeth, blood, oral soft tissue, oral tissue-derived cells, saliva, mouth rinse solution, dental plaque, and gingival crevicular fluid, are described in detail.

Results: The KOBN established SOP for oral biospecimen QC and assessment methods.

Conclusions: To ensure a stable supply of high-quality biospecimens for researchers, regular QC checks should be carried out according to the SOP following the specifications of the Korea Biobank Network under the Korea Disease Control and Prevention Agency.

Purpose: Root coverage (RC) procedures require long-term evaluation. This study assessed the clinical validity and long-term stability of a modified tunneling technique for lower anterior gingival recession (GR) using a subepithelial connective tissue graft (SCTG) and a volume-stable collagen matrix.

Methods: Across 39 patients, 66 mandibular incisors with ≥1.0 mm of GR were examined before and after RC surgery. Clinical photographs documenting the results of RC were taken at baseline (T₀) and the most recent follow-up visit (T_l). Impressions were obtained either at baseline (T₀) or 3 weeks later (T₃). The recession depth, Miller classification, and rates of RC and complete root coverage (CRC) were assessed.

Results: This study analyzed 66 GR sites across 39 patients, with an average follow-up period of 41.3 months. Overall, the mean RC achieved was 86.2%±15.7%. Among single recessions, the RC was 85.2%±25.6% for Miller class I, 91.5%±10.4% for class II, and 79.2%±18.3% for class III. Regarding multiple recessions, the RC was 85.1%±16.2% for Miller class I, 87.0%±12.5% for class II, and 89.8%±16.0% for class III. By Miller classification, the RC was 85.1%±16.8% for class I, 88.7%±11.6% for class II, and 85.8%±17.3% for class III. Furthermore, the RC varied by follow-up duration: 72.5%±15.1% at 12 months, 90.1%±12.6% at 25-36 months, 89.0%±16.7% at 37-48 months, 91.10%±9.88% at 49-60 months, and 97.6±4.79% for longer than 61 months, with 77.8% of the last group achieving CRC. RC also differed based on the initial recession depth, at 88.0%±16.8% for 1-3 mm, 83.1%±14.1% for 3-6 mm, and 80.2%±5.04% for depths exceeding 6 mm.

Conclusions: A modified tunneling technique, utilizing SCTG and a volume-stable collagen matrix, appears to represent a reliable option for the long-term management of GR in the lower anterior region, even in cases involving multiple Miller class III GRs.

Purpose: Self-efficacy is an important factor in the management of chronic oral diseases. This study aimed to develop a Korean version of a self-efficacy tool related to personal oral hygiene management, and verify its validity and reliability.

Methods: This study evaluated the validity and reliability of a Korean version of the oral health-related self-efficacy measurement tool (OHSE-K). The sub-factors of this self-efficacy tool are tooth brushing, interdental hygiene management, and dental visits. The original items were translated into Korean, and their content validity was confirmed. Initially, a preliminary survey was conducted, followed by the main survey. The main survey comprised 19 content-verified items. The validity and reliability of the main survey were evaluated through repeated exploratory factor analyses. A randomly selected sample of Korean adults, aged 19 years or older, completed the OHSE-K online between May 10 and June 2, 2023. The study recruited 400 adults for the preliminary survey. Data were analyzed using PASW 25.0.

Results: The OHSE-K demonstrated a high level of overall reliability (Cronbach's α=0.891). Exploratory factor analysis revealed 3 significant factors: tooth-brushing self-efficacy, interdental hygiene management self-efficacy, and dental visit self-efficacy, with a cumulative explanation rate of 65.114%. The criterion validity results indicated that oral healthcare awareness and behavior, as well as unmet dental treatment needs, were significant (P<0.05). Additionally, the OHSE-K scores showed significant correlations with all 3 sub-factors (P<0.05).

Conclusions: The OHSE-K is a reliable tool. Our results demonstrated its validity and reliability.

Purpose: The Korean Oral Biobank Network (KOBN) collects, stores, and provides oral samples for research. Quality control (QC) of biospecimens is necessary to ensure that they meet the basic prerequisites before being sent to researchers. This study presents the standard operating procedures (SOPs) for the QC of biospecimens.

Methods: QC methods using molecular genetic techniques according to sample types, including teeth, blood, oral soft tissue, oral tissue-derived cells, saliva, mouth rinse solution, dental plaque, and gingival crevicular fluid, are described in detail.

Results: The KOBN established SOP for oral biospecimen QC and assessment methods.

Conclusions: To ensure a stable supply of high-quality biospecimens for researchers, regular QC checks should be carried out according to the SOP following the specifications of the Korea Biobank Network under the Korea Disease Control and Prevention Agency.

Purpose: Periodontitis is an inflammatory disease that results in the loss of periodontal tissue. The endocannabinoid system has anti-inflammatory properties and displays considerable potential for tissue regeneration. In this study, we aimed to explore whether the activation of this system can alleviate or reverse the inflammatory phenotype of human periodontal ligament stem cells (hPDLSCs) induced by exposure to the inflammagen lipopolysaccharide (LPS).

Methods: We investigated the effects of activating specific cannabinoid receptors (CB1 and CB2) on the inflammatory phenotype of LPS-stimulated hPDLSCs. The exogenous ligands WIN55,212-2 and JWH-133 were employed to target the cannabinoid receptors. We conducted a thorough assessment of cell proliferation, metabolic activity, and adipogenic, osteogenic, and chondrogenic differentiation potential. Additionally, we measured cytokine release using enzyme-linked immunosorbent assays.

Results: Exposure to Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) caused an increase in cell proliferation while decreasing metabolic activity. While this exposure did not influence adipogenic or chondrogenic differentiation, it did result in reduced osteogenesis. Additionally, LPS induced the release of interleukin (IL)-6, IL-8, and monocyte chemoattractant protein 1. Immunolabeling revealed the presence of CB1 and CB2 on the cellular membrane, with these receptors playing distinct roles in hPDLSCs. The CB1 agonist WIN55,212-2 was found to increase metabolic activity and promote adipogenic differentiation, whereas the CB2 agonist JWH-133 promoted cell proliferation and osteogenic differentiation. When hPDLSCs were co-exposed to Pg-LPS and CB ligands, JWH-133 slightly ameliorated the inhibition of osteogenic differentiation and suppressed the release of inflammatory cytokines.

Conclusions: This study clarifies the effects of specific CB receptor activation on hPDLCs and the inflammatory phenotype. Stimulation of the endocannabinoid system through the manipulation of endogenous or the application of exogenous cannabinoids in vivo may represent a potent therapeutic option for combating periodontal inflammatory disorders.

Purpose: The nasopalatine canal (NPC) is one of the most significant anatomical markers in the anterior maxillary region. Its location is quite important, especially for implant placement. Therefore, the purpose of this study was to use cone-beam computed tomography (CBCT) to evaluate the morphology, size, and anatomic variations of the NPC.

Methods: A total of 150 individual CBCT images were assessed in various sections to evaluate the dimensions, morphology, and extent of the NPC. Reformatted sagittal images were chosen to classify the shape and course of the canal. The Student's t-test, the χ² test, and Pearson correlation coefficients were used.

Results: The NPC was longer in males than in females, and the mediolateral diameter of the canal was greater in older adults. In sagittal sections, the canal predominantly exhibits a cylindrical shape, while a spindle shape is the least common. In coronal sections, a single shape is most frequently observed, with a Y-shape being the least common. Regarding the orientation of the canal, slanted canals are more prevalent, whereas vertically curved canals are rare. Typically, 2 canal openings are observed, with 4 openings being the least common.

Conclusion: The current study highlights the challenges associated with identifying the anatomical appearance and variations of the NPC. Consequently, possessing a thorough understanding of this anatomy is essential before undertaking any surgical procedures, such as implant placement. This knowledge helps prevent complications such as nerve injury-related loss of sensation, bleeding due to blood vessel injury, and the development of a nasopalatine duct cyst following trauma to the canal during surgery.

Purpose: This research aimed to investigate potential factors associated with the risk of perforation of the labial bone plate, nasal floor, or maxillary sinus floor during immediate implant placement (IIP) in the maxillary premolar area, utilizing a cone-beam computed tomography (CBCT) virtual study.

Methods: CBCT exams from 179 eligible participants, encompassing 716 teeth, were included. Implants were virtually positioned in 2 orientations: along the long axis of the tooth (the prosthetically-driven position) and in an optimal position relative to adjacent anatomical structures (the bone-driven position). Binary logistic regression analysis was employed to assess potential associations between perforation or invasion and various covariates, including sex, age, tooth region, the distance from the tooth apex to the nasal floor or maxillary sinus floor, the angle between the prosthetically- and bone-driven positions (implant-line A angle [ILAA]), and the labial concavity angle (LCA).

Results: The mean ILAA was 18.3°±8.0°, and the angle was significantly larger for the second premolar compared to the first premolar. The mean minimum implant length was 13.0±2.1 mm, with a bone anchorage of 4 mm. The incidence of perforation was 84.1% for the prosthetically-driven position and 40.5% for the bone-driven position. Factors associated with a higher risk of cortical bone wall perforation or invasion of the 2-mm safety margin from surrounding anatomical structures (in the bone-driven position) included female sex, older age, shorter distance from the tooth apex to the nasal cavity/maxillary sinus, and smaller LCA.

Conclusions: A high prevalence of cortical bone wall perforation or invasion of the 2-mm safety margin is anticipated when performing IIP in the maxillary premolar region.

Purpose: This study investigated the effect of implant vertical positioning within alveolar ridge preservation (ARP) sites on implant stability quotient (ISQ) values, which were measured 10 weeks post-implantation.

Methods: Patients who underwent ARP using collagenized deproteinized bovine bone mineral, followed by implant placement in the posterior area, were divided into 2 groups: the within-ARP group and the beyond-ARP group. In the within-ARP group, osteotomy and implant placement occurred within the ARP boundary. In contrast, in the beyond-ARP group, these procedures were performed beyond the ARP boundary, incorporating 3 mm of pristine bone at the implant's apex. Bone quality was assessed by tactile sense, and both insertion torque during implant surgery and ISQ values at 10 weeks post-implant surgery were measured. Multiple linear regression analysis and Pearson correlation analysis were used to explore the relationship between insertion torque and ISQ values.

Results: In total, 30 ARP sites in 28 patients were analyzed. There was no significant difference in bone quality, as determined by tactile sense, between the within-ARP and beyond-ARP groups. At the time of implant placement, the beyond-ARP group exhibited a higher insertion torque (33.33±13.39 Ncm) compared to the within-ARP group (17.08±11.17 Ncm). However, the ISQ values were similar between the 2 groups 10 weeks after implant placement. A positive correlation between insertion torque and ISQ values was confirmed at 10 weeks post-implant.

Conclusions: The engagement of pristine bone may facilitate high insertion torque during the placement of implants in ARP sites. Nevertheless, by 10 weeks post-implantation, the ISQ values were found to be comparable, irrespective of the implant's position.