Journal of Bone Oncology最新文献

{"title":"Pain in patients with multiple inherited osteochondromas: Incidence and potential prognostic factors. A retrospective cohort study","authors":"Morena Tremosini ,&nbsp;Mattia Morri ,&nbsp;Cristiana Forni ,&nbsp;Elena Pedrini ,&nbsp;Marina Mordenti ,&nbsp;Maria Gnoli ,&nbsp;Alessia Di Cecco ,&nbsp;Alice Moroni ,&nbsp;Luca Sangiorgi","doi":"10.1016/j.jbo.2025.100672","DOIUrl":"10.1016/j.jbo.2025.100672","url":null,"abstract":"<div><h3>Purpose</h3><div>the purpose of this study was to describe the baseline characteristics, presenting phenotype and treatment interventions for patients diagnosed with multiple osteochondromas who presented with severe pain symptoms.</div></div><div><h3>Methods</h3><div>a retrospective single-centre cohort study was conducted at a Rare Skeletal Disorders Department. Pain symptomatology was measured at the first visit, pain level was reported, varying from 0, absence of pain to 10, maximum pain. Baseline characteristics, pathology phenotype using IOR classification and treatments performed/ongoing as medical, surgical and conservative therapies were collected.</div></div><div><h3>Results</h3><div>a total of 152 patients were enrolled, with a median pain score of 0 and the 25th and 75th percentiles of 0 and 4, respectively. A percentage of 25.7 % (95 % CI of 19.3–33.3) presented at the first visit with moderate/severe pain. Multiple logistic regression confirmed that age was the only factor to be significantly associated with moderate/severe pain and IOR classification was not able to provide a description of the pathology that was associated with a major pain score.</div></div><div><h3>Conclusion</h3><div>from the early stages of multiple osteochondromas diagnosis<strong>,</strong> pain symptoms must be carefully assessed. An increase in age is associated with a worsening of pain; IOR classification of the multiple osteochondromas phenotype does not currently allow an association between the various classes and pain. A re-evaluation of the classification in this light could be an important new element for clinical practice.</div></div>","PeriodicalId":48806,"journal":{"name":"Journal of Bone Oncology","volume":"52 ","pages":"Article 100672"},"PeriodicalIF":3.4,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143687846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Health- related quality of life after surgery for spinal metastases","authors":"Silvia Terzi ,&nbsp;Cristiana Griffoni ,&nbsp;Simona Rosa ,&nbsp;Chiara Cini ,&nbsp;Emanuela Asunis ,&nbsp;Chiara Alcherigi ,&nbsp;Federica Trentin ,&nbsp;Stefano Bandiera ,&nbsp;Riccardo Ghermandi ,&nbsp;Giuseppe Tedesco ,&nbsp;Gisberto Evangelisti ,&nbsp;Marco Girolami ,&nbsp;Valerio Pipola ,&nbsp;Giovanni Barbanti Brodano ,&nbsp;Alessandro Gasbarrini","doi":"10.1016/j.jbo.2025.100675","DOIUrl":"10.1016/j.jbo.2025.100675","url":null,"abstract":"<div><h3>Background</h3><div>Surgery for spinal metastases is almost always palliative and part of a multidisciplinary approach that has determined a significant increase of life expectancy in the last decade; thus, an improvement in health-related quality of life (HRQOL) is the main goal of the treatment of spinal metastases. We report here the results of a prospective study conducted with the aim of evaluating the impact of spinal surgery on HRQOL, measured by Patient-Reported Outcomes Measures (PROMs). We analyzed clinical outcomes (ambulatory status, performance status, pain, neurological status) and HRQOL scores (VAS, EQ5D, SF36) during the follow-up period and focused on factors that could affect quality of life, considering both psychological and physical issues.</div></div><div><h3>Methods</h3><div>169 patients (96 males, 73 females) with vertebral metastases who underwent surgery at a tertiary referral center were consecutively enrolled from August 2018 to October 2022. Clinical and surgical data were prospectively collected, and PROMs (VAS, EQ-5D and SF-36) were registered before surgery and during follow up.</div></div><div><h3>Results</h3><div>The overall survival was 22 months, and a 61 % survival rate was registered at 1 year follow-up. We observed a significant improvement in walking ability, general performance status, pain and HRQOL after surgery, which was maintained during the follow up. Multivariate analysis identified three independent variables, capable of influencing the trend of HRQOL after surgery: the presence of pathological fracture, the preoperative neurological status and the local recurrence of disease.</div></div><div><h3>Discussion</h3><div>This study confirms the effectiveness of surgery for spinal metastases in improving patients’ performance status and demonstrates an overall improvement in HRQOL, which is maintained over time.</div></div>","PeriodicalId":48806,"journal":{"name":"Journal of Bone Oncology","volume":"52 ","pages":"Article 100675"},"PeriodicalIF":3.4,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143687703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of myosteatosis on prognosis in multiple myeloma patients: A subgroup analysis of 182 cases and development of a nomogram","authors":"Jun-Peng Liu ,&nbsp;Xing-Chen Yao ,&nbsp;Ming Shi ,&nbsp;Zi-Yu Xu ,&nbsp;Yue Wu ,&nbsp;Xiang-Jun Shi ,&nbsp;Meng Li ,&nbsp;Xin-Ru Du","doi":"10.1016/j.jbo.2025.100670","DOIUrl":"10.1016/j.jbo.2025.100670","url":null,"abstract":"<div><h3>Background</h3><div>This study aims to explore the prognostic value of myosteatosis in multiple myeloma (MM) and to analyze the factors influencing myosteatosis.</div></div><div><h3>Methods</h3><div>A retrospective analysis was conducted on 182 patients treated for MM at our institution from 2009 to 2020 who underwent MRI examinations. The fatty infiltration rate (FIR) of the erector spinae and multifidus muscles at the L3 level was measured to assess the degree of myosteatosis. Patients were grouped based on fracture presence and median FIR, and group differences were compared, with P &lt; 0.05 considered statistically significant. Survival and fractures were used as prognostic indicators, and regression analysis was performed to determine the impact of FIR on these outcomes in MM patients. The factors influencing FIR were analyzed, and the relationship between myosteatosis and MM prognosis was further analyzed within its sensitive subgroups. Finally, a nomogram based on FIR was established and validated.</div></div><div><h3>Results</h3><div>Significant differences were observed between the fracture and non-fracture groups in lactate dehydrogenase, serum phosphorus, visual analogue scale, oswestry disability index and FIR (P &lt; 0.05). When patients were grouped based on the median FIR (28.89 %), there were significant differences in age, sex, body mass index (BMI), red blood cell (RBC) count, hemoglobin, hematocrit, albumin, visual analogue scale, oswestry disability index, and fracture incidence (P &lt; 0.05). Univariate COX regression analysis indicated that myosteatosis had no significant impact on survival prognosis in MM patients (HR = 0.999, P = 0.852), with a log-rank test P value of 0.11 when grouped by the cut-off FIR value of 33.67 %. Multivariate logistic regression indicated that FIR is an independent predictor of fractures (OR = 1.054, P = 0.000). Multivariate linear regression revealed that age, sex, RBC count, and BMI are independent factors influencing FIR (P &lt; 0.05). When not grouped, FIR’s prediction of fractures showed no significant interaction with age, sex, RBC count, or BMI (P for interaction &gt; 0.05). In subgroups with BMI ≥ 25 kg/m² or RBC count &gt; 3.68 × 10^12/L, FIR lost its predictive significance for fractures. The FIR nomogram model had a C-index of 0.777, and the calibration curve, decision curve analysis, and clinical impact curve all validated its effectiveness.</div></div><div><h3>Conclusions</h3><div>Myosteatosis characterized by FIR is not a reliable predictor of survival in MM patients but is effective in predicting fractures and is closely related to back pain and functional impairment. FIR is significantly associated with age, sex, RBC count, and BMI.</div></div>","PeriodicalId":48806,"journal":{"name":"Journal of Bone Oncology","volume":"51 ","pages":"Article 100670"},"PeriodicalIF":3.4,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143621301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic insights into bone destruction in multiple myeloma: Cellular and molecular perspectives","authors":"Oxana Lungu ,&nbsp;Denise Toscani ,&nbsp;Nicola Giuliani","doi":"10.1016/j.jbo.2025.100668","DOIUrl":"10.1016/j.jbo.2025.100668","url":null,"abstract":"<div><div>Multiple myeloma (MM) is a hematological malignancy that leads to significant bone destruction, resulting in debilitating pain and skeletal-related events. The pathophysiology of osteolytic bone destruction in MM involves complex interactions between malignant plasma cells (PCs) and the bone marrow (BM) microenvironment. This review aims to provide a comprehensive synthesis of the cellular and molecular pathways underlying MM-associated bone disease. We discuss the role of osteoclast (OC), osteoblast (OB), osteocytes, along with the complex interactions between immune cells and the BM microenvironment in shaping disease progression. Additionally, we explore the molecular signaling pathways involved in bone disease as well as the influence of inflammatory cytokines, and the role of the metabolic alterations that characterize the MM BM. We also explore novel therapeutic strategies targeting these pathways to improve clinical outcomes. Understanding these mechanisms is crucial for the development of more effective treatments to prevent bone damage in MM patients.</div></div>","PeriodicalId":48806,"journal":{"name":"Journal of Bone Oncology","volume":"51 ","pages":"Article 100668"},"PeriodicalIF":3.4,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Innovations: Advances in imaging techniques for diagnosis and follow-up of multiple myeloma","authors":"M. Talarico ,&nbsp;S. Barbato ,&nbsp;A. Cattabriga ,&nbsp;I. Sacchetti ,&nbsp;E. Manzato ,&nbsp;R. Restuccia ,&nbsp;S. Masci ,&nbsp;F. Bigi ,&nbsp;M. Puppi ,&nbsp;M. Iezza ,&nbsp;I. Rizzello ,&nbsp;K. Mancuso ,&nbsp;L. Pantani ,&nbsp;P. Tacchetti ,&nbsp;C. Nanni ,&nbsp;M. Cavo ,&nbsp;E. Zamagni","doi":"10.1016/j.jbo.2025.100669","DOIUrl":"10.1016/j.jbo.2025.100669","url":null,"abstract":"<div><h3>Introduction</h3><div>The International Myeloma Working Group (IMWG) defines myeloma related bone disease (MBD) as a diagnostic criterion for symptomatic multiple myeloma (MM) as the presence of osteolytic lesions ≥ 5 mm or more than one focal lesion (FL) ≥ 5 mm by magnetic resonance imaging (MRI). Whole-body low-dose CT (WBLDCT) is recommended as the first-choice imaging technique for the diagnosis of MBD with ¹⁸F-fluorodeoxyglucose-positron emission tomography/CT (¹⁸F-FDG-PET/CT) being considered a possible alternative at staging, whereas use of MRI studies is recommended in cases without myeloma-defining events (MDEs) in order to exclude the presence of FLs. Furthermore, use of ¹⁸F-FDG-PET/CT is recommended in response assessment, to be integrated with hematologic response and bone marrow minimal residual disease (MRD).</div></div><div><h3>Areas covered</h3><div>In this paper, we review novel functional imaging techniques in MM, particularly focusing on their advantages, limits, applications and comparisons with ¹⁸F-FDG-PET/CT or other standardized imaging techniques.</div></div><div><h3>Conclusions</h3><div>Combining both morphological and functional imaging, ¹⁸F-FDG-PET/CT is currently considered a standard imaging technique in MM for staging (despite false positive or negative results) and response assessment. The introduction of novel functional imaging techniques, as whole-body diffusion-weighted magnetic resonance imaging (WB-DWI-MRI), or novel PET tracers might be useful in overcoming these limits. Future studies will give more information on the complementarity of these imaging techniques or whether one of them might become a new gold standard in MM.</div></div>","PeriodicalId":48806,"journal":{"name":"Journal of Bone Oncology","volume":"51 ","pages":"Article 100669"},"PeriodicalIF":3.4,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143563646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zoledronic acid in metastatic castrate-sensitive prostate cancer: A state-of-the-art review","authors":"Nahed Damaj,&nbsp;Tala Najdi,&nbsp;Samah Seif,&nbsp;Nicolas Nakouzi,&nbsp;Joseph kattan","doi":"10.1016/j.jbo.2025.100667","DOIUrl":"10.1016/j.jbo.2025.100667","url":null,"abstract":"<div><div>Prostate cancer is the most common cancer in men in developed countries. Despite its slow growing pattern, metastatic disease to bone occurs and results in a significant number of deaths. Since more than eight decades, the classical androgen deprivation therapy (ADT) leads to clinical response in most patients with metastatic castration-sensitive prostate cancer (mCSPC). Moving backward docetaxel and androgen receptor pathway inhibitors (ARPI) from castrate-resistant setting to castrate sensitive setting improves overall survival (OS) compared to ADT alone. Recently, studies suggested that triplet therapy by adding ARPIs such as abiraterone acetate or darolutamide to ADT + docetaxel is more effective than ADT/docetaxel alone in patients with high-volume mCSPC. Although the scientific progress during the last decade, has led to improvements in outcome for patients with mCSPC, there are still several areas impacting daily practice, for which high-level evidence is lacking, especially for adding monthly zoledronic acid in this setting. We structured this review by conducting a comprehensive analysis of the existing literature. This manuscript reviews both the benefits and potential harms of zoledronic acid in the treatment of mCSPC and provides conclusions on the criteria for its use, and the possible use of alternative bone protecting agents (BPA).</div></div>","PeriodicalId":48806,"journal":{"name":"Journal of Bone Oncology","volume":"51 ","pages":"Article 100667"},"PeriodicalIF":3.4,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143600682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Faster R-CNN model for target recognition and diagnosis of scapular fractures","authors":"Qiong Fang ,&nbsp;Anhong Jiang ,&nbsp;Meimei Liu ,&nbsp;Sen Zhao","doi":"10.1016/j.jbo.2025.100664","DOIUrl":"10.1016/j.jbo.2025.100664","url":null,"abstract":"<div><h3>Objective</h3><div>This study aims to establish a diagnostic model for scapular fractures using a convolutional neural network (CNN) and to discuss the clinical advantages of this model in diagnosing such complex conditions.</div></div><div><h3>Methods</h3><div>Computed tomography (CT) images of 90 patients with scapular fractures were collected. A faster R-CNN-based recognition model was developed and compared with manual diagnosis. External validation was conducted to evaluate the model’s accuracy, sensitivity, specificity, positive predictive value, and negative predictive value.</div></div><div><h3>Results</h3><div>The CNN model, when combined with medical expert interpretation, demonstrated significantly higher specificity and positive predictive value compared to orthopedist-independent interpretation and algorithm-independent prediction (P &lt; 0.05). The area under the curve (AUC) value of the combined approach was significantly higher than that of orthopedist-independent interpretation and algorithm-independent prediction groups, with statistically significant differences (P &lt; 0.05). The accuracy of the CNN algorithm model combined with orthopedist interpretation was 97.78 %, significantly higher than orthopedist-independent interpretation (82.95 %) and CNN algorithm-independent prediction (92.05 %) (P &lt; 0.05).</div></div><div><h3>Conclusions</h3><div>The CNN-based recognition model for scapular fractures can assist clinicians in improving their diagnostic accuracy and precision in identifying such fractures on CT images.</div></div>","PeriodicalId":48806,"journal":{"name":"Journal of Bone Oncology","volume":"51 ","pages":"Article 100664"},"PeriodicalIF":3.4,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular pathological insights into tumorigenesis and progression of giant cell tumor of bone","authors":"Yibing Yao ,&nbsp;Victor Kwan Min Lee ,&nbsp;Ee Sin Chen","doi":"10.1016/j.jbo.2025.100665","DOIUrl":"10.1016/j.jbo.2025.100665","url":null,"abstract":"<div><div>Giant cell tumor of bone (GCTB) is a primary bone tumor that typically exhibits benign histological appearance and clinical behavior in most cases, with local aggressiveness and rare metastasis. It predominantly affects individuals in the young adult age group. It is characterized by the presence of multinucleated osteoclastic giant cells and a stromal population of neoplastic cells. A key hallmark for GCTB pathogenesis is the G34W genetic mutation in the histone H3.3 gene, which is restricted to the population of cancerous stromal cells and is absent in osteoclasts and their progenitor cells. This review presents a comprehensive overview of the pathology of GCTB, including its histopathological characteristics, cytological features, histopathological variants, and their clinical relevance. We also discuss recent insights into genetic alterations in relation to the molecular pathways implicated in GCTB. A summary of the current understanding of GCTB pathology will update the knowledge base to guide the diagnosis and management of this unique bone tumor.</div></div>","PeriodicalId":48806,"journal":{"name":"Journal of Bone Oncology","volume":"51 ","pages":"Article 100665"},"PeriodicalIF":3.4,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility of ADC histogram analysis for predicting of postoperative recurrence in aggressive spinal tumors","authors":"Qizheng Wang ,&nbsp;Yongye Chen ,&nbsp;Guangjin Zhou ,&nbsp;Tongyu Wang ,&nbsp;Jingchao Fang ,&nbsp;Ke Liu ,&nbsp;Siyuan Qin ,&nbsp;Weili Zhao ,&nbsp;Dapeng Hao ,&nbsp;Ning Lang","doi":"10.1016/j.jbo.2025.100666","DOIUrl":"10.1016/j.jbo.2025.100666","url":null,"abstract":"<div><h3>Background</h3><div>Risk stratification of spinal tumors is a major unmet clinical need for personalized therapy.</div></div><div><h3>Purpose</h3><div>To explore the feasibility of pretreatment whole-lesion apparent diffusion coefficient (ADC) histogram in predicting local recurrence of aggressive spinal tumors.</div></div><div><h3>Methods</h3><div>119 aggressive spinal tumor patients (median age, 40; range, 13–74  years) confirmed by pathological findings with a mean follow-up of 36 months were enrolled and divided into the recurrence and non-recurrence group. The histogram metrics of whole-lesion, including the maximum, mean, kurtosis, skewness, entropy, and percentiles (10th, 25th, 50th, 75th, 95th) ADC values, were evaluated and take the average. Fractal dimension (FD) was assessed in the three orthogonal directions and take maximum. Clinical and general imaging features were used to construct an alternative prognostic model for comparison. Variables with statistical differences would be included in stepwise logistic regression analysis.</div></div><div><h3>Results</h3><div>As for the clinical model, Enneking staging (odds ratio [OR]: 3.572; <em>P</em> = 0.04) and vertebral compression (OR: 4.302; <em>P</em> = 0.002) were independent predictors of recurrence. There was no statistical difference in FD between the two groups (<em>P</em> = 0.623). Among the ADC histogram parameters compared, skewness, maximum, and mean ADC values were independent risk factors and constructed ADC histogram prediction models. The ADC histogram model (AUC = 0.871) and the combined model (AUC = 0.884) performed better than the clinical prediction model (AUC = 0.704) with <em>P</em>-values of 0.004 and 0.001, respectively.</div></div><div><h3>Conclusion</h3><div>Prediction models based on the ADC histogram analysis might represent serviceable instruments for the aggressive spinal tumors.</div></div>","PeriodicalId":48806,"journal":{"name":"Journal of Bone Oncology","volume":"51 ","pages":"Article 100666"},"PeriodicalIF":3.4,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The METTL3/TGF-β1 signaling axis promotes osteosarcoma progression by inducing MSC differentiation into CAFs via m6A modification","authors":"Jin Qi ,&nbsp;Sihang Liu ,&nbsp;Baomin Wu ,&nbsp;Gang Xue","doi":"10.1016/j.jbo.2025.100662","DOIUrl":"10.1016/j.jbo.2025.100662","url":null,"abstract":"<div><div>Osteosarcoma, a prevalent and aggressive skeletal malignancy, significantly impacts the prognosis of individuals, particularly young patients. Current treatments, including surgery and chemotherapy, often prove inadequate for advanced osteosarcoma with metastasis. This study investigates the role of the METTL3/TGF-β1 signaling axis in promoting osteosarcoma progression by inducing mesenchymal stem cells (MSCs) to differentiate into cancer-associated fibroblasts (CAFs). Utilizing co-culture technology, we demonstrated that osteosarcoma cells secrete TGF-β1, which is crucial for MSC differentiation into CAFs, as evidenced by the increased expression of CAF markers α-SMA, FSP-1, and FAP. Additionally, METTL3 was found to enhance the stability and expression of TGF-β1 mRNA through m⁶A modification, thereby facilitating the differentiation process of MSCs. <em>In vivo</em> xenograft experiments further confirmed that the METTL3/TGF-β1 axis significantly promotes tumor growth in osteosarcoma by mediating the differentiation of MSCs into CAFs. These findings provide new insights into the molecular mechanisms underlying osteosarcoma progression and highlight potential therapeutic targets for treating advanced stages of this malignancy.</div></div>","PeriodicalId":48806,"journal":{"name":"Journal of Bone Oncology","volume":"51 ","pages":"Article 100662"},"PeriodicalIF":3.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}