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Craniofacial morphology of patients with unilateral cleft lip and palate at two stages of skeletal maturation. 单侧唇腭裂患者骨骼成熟两个阶段的颅面形态。
IF 2.5 4区 医学 Q2 Dentistry Pub Date : 2023-01-01 DOI: 10.1590/1807-3107bor-2023.vol37.0009
Eduardo Murad Villoria, Bernardo Quiroga Souki, Flávio Lucena Antunes, Marina Araújo Leite Assis, Ildeu Andrade Júnior, Dauro Douglas Oliveira, Rodrigo Villamarim Soares

The aim of this retrospective cross-sectional investigation was to perform a 3D analysis of craniofacial morphology of patients with unilateral cleft lip and palate (UCLP) at two stages of skeletal maturation. Cone-beam computed tomography (CBCT) scans of 52 UCLP patients (34 prepubertal; 18 pubertal) were collected from an outpatient referral center for the treatment of craniofacial deformities. In total 15 multiplanar craniofacial landmarks were identified, 3D virtual surface models were created, and 13 variables were measured to assess the 3D Euclidean distances between landmarks and spatial position of the landmarks in the projected X, Y and Z components. Maxillary and mandibular pitch (clockwise, counterclockwise) rotation relative to the cranial base was also evaluated. The significance level was set at 5%. Maxillary retrusion value relative to the cranial base was higher and statistically significant greater (p = 0.028) in pubertal (SNA, 77.4° ± 6.2; N-ANS Y, 3.3 mm ± 3.1) than in prepubertal patients (SNA 81.0° ± 5.2; N-ANS Y, 5.8 mm ± 2.7). The posterior cranial base length (S-Ba Y) was significantly longer (p = 0.013) in pubertal (20.7 mm ± 3.4) than in prepubertal patients (18.4 mm ± 2.7). The upper facial height (N-ANS Z) was significantly greater (p = 0.01) in pubertal (46.9 mm ± 4.5) than in prepubertal patients (43.4 mm ± 3.0). Prepubertal and pubertal UCLP patients presented distinct patterns of craniofacial morphology, mainly in the sagittal component of the maxilla and in the posterior cranial base length. Pubertal patients had greater maxillary retrusion and posterior cranial base length.

本回顾性横断面研究的目的是在骨骼成熟的两个阶段对单侧唇腭裂(UCLP)患者的颅面形态进行三维分析。锥形束ct (CBCT)扫描52例UCLP患者(34例青春期前;从门诊转诊中心收集治疗颅面畸形的患者。总共识别了15个多平面颅面地标,创建了三维虚拟表面模型,并测量了13个变量来评估地标之间的三维欧几里得距离以及地标在投影X, Y和Z分量中的空间位置。还评估了上颌和下颌相对于颅底的螺距(顺时针、逆时针)旋转。显著性水平设为5%。上颌后缩值相对于颅底较高,青春期(SNA, 77.4°±6.2)差异有统计学意义(p = 0.028);N-ANS Y, 3.3 mm±3.1)高于青春期前患者(SNA 81.0°±5.2;N-ANS Y, 5.8 mm±2.7)。青春期患者后颅底长度(S-Ba - Y) (20.7 mm±3.4)明显长于青春期前患者(18.4 mm±2.7)(p = 0.013)。青春期患者上面部高度(N-ANS - Z) (46.9 mm±4.5)显著高于青春期前患者(43.4 mm±3.0),差异有统计学意义(p = 0.01)。青春期前和青春期UCLP患者的颅面形态不同,主要表现在上颌骨矢状面部分和后颅底长度。青春期患者上颌后缩较大,后颅底长度较大。
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引用次数: 0
Assessment of the contextual effects on the prevalence of periodontitis: a systematic review. 评估对牙周炎流行的环境影响:一项系统综述。
IF 2.5 4区 医学 Q2 Dentistry Pub Date : 2023-01-01 DOI: 10.1590/1807-3107bor-2022.vol36.0125
Leonardo Vilar Filgueiras, Willian Konflanz, Alex Nogueira Haas, Roger Keller Celeste

This study reviews the influence of contextual factors on periodontitis based on a systematic search of studies recorded in the PubMed, Scopus, Web of Science, and EMBASE databases. Periodontitis was assessed by clinical attachment loss and probing depth for studies with data on the socioeconomic status (SES) of a specific area (area-level SES) or dental care service (service-level) in a catchment area among individuals aged 18 and over. Two independent reviewers performed study selection, data extraction, and assessment of methodological quality. Of the 646 articles identified, 13 were included in the systematic review, representing 101,362 individuals from five countries (USA, UK, Brazil, China, and Uruguay). A higher prevalence of periodontitis was described in lower SES neighborhoods, more deprived postcodes, and poorer provinces. Gini Index results were mixed and inconclusive. Three studies showed that higher coverage of primary dental care at the municipal level was associated with a lower prevalence of periodontitis. Contextual factors at the area-level SES and dental care service might influence periodontitis, but the existing evidence is unclear. The contextual effect is important for periodontal health and may contribute to the prevalence of periodontitis, independent of well-known risk factors and individual levels.

本研究通过对PubMed、Scopus、Web of Science和EMBASE数据库中记录的研究进行系统检索,回顾了环境因素对牙周炎的影响。牙周炎是通过临床附着丧失和探测深度来评估的,研究数据包括特定区域(区域级SES)的社会经济地位(SES)或集水区18岁及以上个体的牙科保健服务(服务水平)。两名独立审稿人进行了研究选择、数据提取和方法学质量评估。在确定的646篇文章中,13篇被纳入系统评价,代表来自5个国家(美国、英国、巴西、中国和乌拉圭)的101362人。在社会经济地位较低的社区、较贫困的邮政编码和较贫穷的省份,牙周炎的患病率较高。基尼指数的结果好坏参半,尚无定论。三项研究表明,市政一级初级牙科保健覆盖率较高与牙周炎患病率较低有关。背景因素在地区水平的SES和牙科保健服务可能影响牙周炎,但现有的证据尚不清楚。环境影响对牙周健康很重要,并可能导致牙周炎的流行,独立于已知的危险因素和个人水平。
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引用次数: 1
Comparative study of sex estimates in adult skulls using direct measurement and tomographic image reconstruction. 成人颅骨性别直接测量与断层图像重建之比较研究。
IF 2.5 4区 医学 Q2 Dentistry Pub Date : 2023-01-01 DOI: 10.1590/1807-3107bor-2023.vol37.0064
Ezequiel Ortiz Rosa, Edgard Michel Crosato, Claudio Campi Castro, Rodrigo Elias Oliveira, Maria Gabriela Haye Biazevic

Sex estimation is an important procedure in forensic anthropology for human identification. The development of new technologies, such as three-dimensional computed tomography (CT), has provided excellent alternatives for this purpose. This study examined and compared a morphological method for sex estimation using two different approaches - direct measurement of physical structures and tomographic analysis using 3D images. A total of 111 skulls from the Museum of Human Anatomy at the University of São Paulo (MAH-USP) were used, (60 males and 51 females). All specimens were scanned by Philips Brilliance 64 CT scanner equipment and their corresponding images were reconstructed in three-dimensional (3D) models. The morphological characteristics of the skulls were analyzed by an observer who was blinded to the sex of the specimens. Five cranial structures were analyzed: external occipital crest, mastoid process, supraorbital margin, glabella, and mental eminence. The structures were scored 1 to 5 according to Buikstra and Ubelaker and validated by Walker. The success rates of the sex estimates obtained through direct measurement of the dry skulls ranged from 67.4% to 70.4% as compared to 60.2% to 68.1% for CT reconstruction. When analyzed separately, the maximum accuracy of the method was 68.33% in males and 88.24% in females in the physical analysis of structures. The glabella and mastoid process were the most effective structures to estimate sex through both techniques, respectively. Our results show that 3D CT images can be accurately used in the morphological analysis for sex estimation, representing a viable alternative in forensic anthropology.

性别鉴定是法医人类学中人类身份鉴定的重要环节。新技术的发展,如三维计算机断层扫描(CT),为这一目的提供了极好的替代方案。本研究使用两种不同的方法——直接测量身体结构和使用3D图像的层析分析——检验并比较了一种用于性别估计的形态学方法。总共使用了来自圣保罗大学人体解剖学博物馆(MAH-USP)的111个头骨(60个男性和51个女性)。采用Philips Brilliance 64 CT扫描设备对所有标本进行扫描,并将相应图像重建为三维模型。头骨的形态特征是由一个不知道标本性别的观察者分析的。我们分析了五个颅骨结构:枕外嵴、乳突、眶上缘、眉间窝和精神隆起。根据Buikstra和Ubelaker评分为1到5分,并经Walker验证。通过直接测量干颅骨获得的性别估计成功率为67.4%至70.4%,而CT重建的成功率为60.2%至68.1%。单独分析时,该方法在结构物理分析中,男性的最高准确率为68.33%,女性的最高准确率为88.24%。通过这两种技术,眉骨和乳突分别是估计性别最有效的结构。我们的研究结果表明,3D CT图像可以准确地用于形态学分析的性别估计,代表了法医人类学的一个可行的选择。
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引用次数: 1
Associations between physical activity domains and oral health: an analysis of a Brazilian population-based study. 体育活动领域与口腔健康之间的关系:一项基于巴西人群的研究分析
IF 2.5 4区 医学 Q2 Dentistry Pub Date : 2023-01-01 DOI: 10.1590/1807-3107bor-2023.vol37.0071
Stefany Duarte Dos Anjos, Rafael Milani Ferro, Bernardo Novak Laskawski, Alex Nogueira Haas, Rodolfo Coelho Prates, Joao Paulo Steffens

Physical activity comprises four domains (leisure, transportation, domestic, and work activities) that may be differently associated with oral health. The aim of this study was to assess the association between each physical activity domain and oral health conditions in Brazilian adults. A total of 38,539 participants in the 2019 Brazilian Health Survey aged 30 years or older were analyzed. The outcomes were self-perceived oral health (dichotomous) and self-reported number of missing teeth (counts). The presence, frequency, and time of activity in each domain and their combination were analyzed as main exposures. Odds ratios (OR) and mean ratios (MR) were estimated by fitting multivariable models. Higher leisure time physical activity was the only domain associated with better self-perception of oral health (OR = 1.32; 95%CI 1.26-1.38) and lower tooth loss (MR=0.88; 95%CI 0.86-0.90). Higher levels of work, transportation, and household activities were significantly associated with worse self-perception of oral health, while higher levels of work- and transportation-related physical activities were also associated with greater tooth loss. When the total recommended weekly physical activity time was analyzed, no significant associations were found. Sensitivity analysis suggested that this pattern persists in potential periodontitis-related cases, such as when selecting older age or excluding individuals with no tooth loss. In conclusion, leisure physical activity was the only domain with the potential of reflecting the benefits of physical activity on oral health. The inclusion of other domains can confound this association.

身体活动包括四个领域(休闲、交通、家庭和工作活动),它们可能与口腔健康有不同的关联。本研究的目的是评估巴西成年人每个体育活动领域与口腔健康状况之间的关系。在2019年巴西健康调查中,共有38,539名年龄在30岁或以上的参与者进行了分析。结果是自我感知的口腔健康(二分法)和自我报告的缺牙数(计数)。分析各脑区活动的存在、频率和时间以及它们的组合作为主要暴露。通过拟合多变量模型估计优势比(OR)和平均比(MR)。较高的休闲时间体力活动是与更好的口腔健康自我感知相关的唯一领域(OR = 1.32;95%CI 1.26-1.38)和下牙脱落(MR=0.88;95%可信区间0.86 - -0.90)。高水平的工作、交通和家庭活动与较差的口腔健康自我认知显著相关,而高水平的工作和交通相关的身体活动也与更大的牙齿脱落有关。当分析推荐的每周体育活动总时间时,没有发现显著的关联。敏感性分析表明,这种模式在潜在的牙周炎相关病例中持续存在,例如当选择老年人或排除没有牙齿脱落的个体时。总之,休闲体育活动是唯一可能反映体育活动对口腔健康益处的领域。包含其他域可能会混淆这种关联。
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引用次数: 0
Do patients with molar incisor hypomineralization have more dental anxiety and behavior management problems? A systematic review with meta-analysis. 臼齿低矿化患者是否有更多的牙齿焦虑和行为管理问题?荟萃分析的系统综述。
IF 2.5 4区 医学 Q2 Dentistry Pub Date : 2023-01-01 DOI: 10.1590/1807-3107bor-2023.vol37.0069
Patrícia Papoula Gorni Reis, Roberta Costa Jorge, Daniele Masterson Tavares Pereira Ferreira, Guido Artemio Marañón-Vásquez, Lucianne Cople Maia, Vera Mendes Soviero

This systematic review evaluated the available evidence on whether children with molar incisor hypomineralization (MIH) have more dental fear and anxiety (DFA) and dental behavior management problems (DBMPs) than those without MIH (Prospero CDR42020203851). Unrestricted searches were performed in PubMed, Scopus, Web of Science, Lilacs, BBO, Embase, Cochrane Library, APA PsycINFO, Open Grey, and Google Scholar. Observational studies evaluating DFA and/or DBMPs in patients with and without MIH were eligible. Reviews, case reports, interventional studies, and those based on questionnaires to dentists were excluded. The methodological quality assessment was based on the Newcastle-Ottawa Scale. Random-effects meta-analyses were conducted to synthesize data on DFA. The certainty of evidence was performed according to GRADE. Seven studies that evaluated a total of 3,805 patients were included. All of them presented methodological issues, mainly in the comparability domain. Most studies observed no significant difference in DFA between children with and without MIH. The meta-analysis did not show a significant effect of MIH on the standardized units for the DFA scores (SMD = 0.03; 95%CI: -0.06-0.12; p = 0.53; I2 = 0%). Synthesis including only the results for severe cases of MIH also did not show a significant effect of the condition on DFA scores (MD = 8.68; 95%CI: -8.64-26.00; p = 0.33; I2 = 93%). Two articles found DBMPs were significantly more frequent in patients with MIH. The overall certainty of evidence was very low for both outcomes assessed. The current evidence suggests no difference in DFA between children with and without MIH; DBMPs are more common in patients with MIH. This information should be viewed with caution because of the very low quality evidence obtained.

本系统评价了有磨牙切牙低矿化(MIH)的儿童是否比没有MIH的儿童有更多的牙科恐惧和焦虑(DFA)和牙科行为管理问题(DBMPs)的现有证据(Prospero CDR42020203851)。在PubMed、Scopus、Web of Science、Lilacs、BBO、Embase、Cochrane Library、APA PsycINFO、Open Grey和Google Scholar中进行无限制搜索。评估伴有和不伴有MIH的患者DFA和/或dbmp的观察性研究是合格的。综述、病例报告、介入性研究和基于牙医问卷的研究均被排除在外。质量评估方法基于纽卡斯尔-渥太华量表。随机效应荟萃分析用于综合DFA的数据。证据的确定性根据GRADE进行评估。纳入了7项研究,共评估了3805名患者。所有这些都提出了方法论问题,主要是在可比性领域。大多数研究发现,有和没有MIH的儿童的DFA没有显著差异。meta分析未显示MIH对DFA评分的标准化单位有显著影响(SMD = 0.03;95%置信区间:-0.06—-0.12;P = 0.53;I2 = 0%)。仅包括严重MIH病例的综合结果也未显示病情对DFA评分的显着影响(MD = 8.68;95%置信区间:-8.64—-26.00;P = 0.33;I2 = 93%)。两篇文章发现dbmp在MIH患者中明显更常见。两种评估结果的证据的总体确定性都很低。目前的证据表明,患有和不患有MIH的儿童的DFA没有差异;dbmp在MIH患者中更为常见。由于所获得的证据质量非常低,因此应谨慎看待这一信息。
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引用次数: 0
Parental oral health literacy influences preschool children's utilization of dental services. 父母口腔健康素养影响学龄前儿童对牙科服务的利用。
IF 2.5 4区 医学 Q2 Dentistry Pub Date : 2023-01-01 DOI: 10.1590/1807-3107BOR-2023.vol37.0090
Bruna Leticia Vessoni Menoncin, Aline Fabris de Araujo Crema, Fernanda de Morais Ferreira, Andrea Ferreira Zandoná, Jose Vitor Nogara Borges de Menezes, Fabian Calixto Fraiz

Parental behavior towards their children's oral health is strongly influenced by parent's Oral Health Literacy (OHL) level. This study evaluated the impact of parental OHL on preschool children's utilization of dental services. A cross-sectional study was conducted with parents of 419 children aged 3 to 5 years who answered a self-administered questionnaire about their perception of their children's oral health and whether their children had already been to a dental visit. Parental OHL level was assessed by the validated version of the Oral Health Literacy Adult Questionnaire (OHL-AQ) translated into Brazilian Portuguese. Univariate and multivariate Poisson regression analyses with robust variance were used for the data analysis (α = 0.05). Most children had already been to a dental visit (73%). Overall, 31.7% of the parents or guardians reported that their children had experienced dental pain or dental caries. Parental OHL level was classified by tercile as low, medium, and high. The final model showed independent associations between children who had already been to a dental visit and higher parental OHL level (PR = 1.16; 95%CI = 1.00-1.35) when compared to the lowest OHL level, higher parental educational level (PR = 1.39; 95%CI = 1.03-1.87) when compared to lower educational level, the report of children's pain and dental caries (PR = 1.22; 95%CI = 1.09-1.36) and married parents or parents in a common-law marriage (PR = 1.17; 95%CI = 1.03-1.93). The prevalence of children who had already been to a dental visit was higher among those parents with a higher OHL level when compared to those with a lower OHL level.

父母口腔健康素养水平对儿童口腔健康行为有显著影响。本研究旨在评估父母职业健康水平对学龄前儿童牙科服务利用的影响。对419名3至5岁儿童的父母进行了一项横断面研究,他们回答了一份关于他们对孩子口腔健康的看法以及他们的孩子是否已经去看牙医的自我管理问卷。父母OHL水平通过翻译成巴西葡萄牙语的口腔健康素养成人问卷(OHL- aq)进行评估。数据分析采用单因素和多因素泊松回归分析(α = 0.05)。大多数儿童已经看过牙医(73%)。总体而言,31.7%的家长或监护人报告他们的孩子有牙痛或蛀牙。亲本OHL水平按不育等级分为低、中、高。最终模型显示,已经看过牙医的儿童与父母较高的OHL水平之间存在独立关联(PR = 1.16;95%CI = 1.00-1.35),与最低OHL水平、较高父母受教育程度(PR = 1.39;95%CI = 1.03-1.87),与教育程度较低的儿童相比,儿童疼痛和龋齿的报告(PR = 1.22;95%CI = 1.09-1.36)和已婚父母或普通法婚姻中的父母(PR = 1.17;95%ci = 1.03-1.93)。与OHL水平较低的父母相比,OHL水平较高的父母中已经看过牙医的儿童的患病率较高。
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引用次数: 0
Sense of coherence and oral health-related quality of life among southern Brazilian male adolescents 巴西南部男性青少年的连贯感和口腔健康相关生活质量
4区 医学 Q2 Dentistry Pub Date : 2023-01-01 DOI: 10.1590/1807-3107bor-2023.vol37.0113
Nicássia Cioquetta LOCK, Maria Laura Castro Alves Ribeiro GAZOLA, Patrícia Kolling MARQUEZAN, Julio Eduardo do Amaral ZENKNER, Luana Severo ALVES
This study evaluated the association between sense of coherence (SoC) and oral health-related quality of life (OHRQoL) among conscripts of the Brazilian Army, in two cities of southern Brazil. A cross-sectional study included all 18-19-year-old adolescents who joined the Brazilian Army as draftees for mandatory military service in the cities of Itaqui, RS, and Santiago, RS (n = 505). Data collection was conducted from 2019 to 2021, and included the application of questionnaires and a clinical oral examination to record gingivitis, malocclusion, and dental caries. OHRQoL was collected through the Brazilian short version of the Oral Health Impact Profile (OHIP), composed of 14 questions. The adolescents’ SoC was assessed using the validated Brazilian version of the SOC-13 scale. The primary outcome of this study was OHRQoL, modeled as a discrete variable (OHIP-14 scores). The main predictor variable was SoC, categorized as low, moderate, or high. The association between predictor variables and OHRQoL was assessed by Poisson regression models using a hierarchical approach. Unadjusted and adjusted rate ratios (RR), and 95% confidence intervals (CI) were estimated. All the analyses were performed using STATA software version 14.2. Adolescents with a moderate and high SoC had 27% (RR = 0.73, 95%CI = 0.64–0.84) and 51% (RR = 0.49, 95%CI = 0.41–0.58) lower mean OHIP-14 scores, respectively, than those with a low SoC score, after the inclusion of behavioral and clinical variables. This study showed a significant association between SoC and OHRQoL among 18–19-year-old southern Brazilian adolescents. Strengthening the SoC as a psychosocial resource may improve the well-being and OHRQoL of adolescents.
本研究评估了巴西南部两个城市巴西军队应征入伍者的连贯感(SoC)与口腔健康相关生活质量(OHRQoL)之间的关系。横断面研究纳入了巴西伊塔基市和圣地亚哥市所有18-19岁的青少年,这些青少年作为义务兵役的应征入伍者加入了巴西军队(n = 505)。数据收集于2019年至2021年进行,包括使用问卷调查和临床口腔检查,记录牙龈炎、错颌合和龋齿。OHRQoL是通过巴西口腔健康影响概况(OHIP)的简短版本收集的,由14个问题组成。使用经过验证的巴西版SoC -13量表对青少年的SoC进行评估。本研究的主要结局是OHRQoL,建模为离散变量(OHIP-14评分)。主要预测变量是SoC,分为低、中、高三个等级。预测变量与OHRQoL之间的关系通过泊松回归模型采用分层方法进行评估。估计未调整和调整的率比(RR)和95%置信区间(CI)。所有分析均使用STATA软件14.2版进行。在纳入行为和临床变量后,中度和高SoC青少年的平均OHIP-14得分分别比低SoC青少年低27% (RR = 0.73, 95%CI = 0.64-0.84)和51% (RR = 0.49, 95%CI = 0.41-0.58)。本研究显示18 - 19岁巴西南部青少年SoC与OHRQoL之间存在显著相关性。加强社会归属感作为一种社会心理资源可以改善青少年的幸福感和OHRQoL。
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引用次数: 0
Effect of thickness and shade of CAD/CAM composite on the light transmission from different light-curing units CAD/CAM复合材料厚度和色度对不同光固化单元透光性的影响
4区 医学 Q2 Dentistry Pub Date : 2023-01-01 DOI: 10.1590/1807-3107bor-2023.vol37.0114
Julia Dantas MAZÃO, Maria Tereza Hordones RIBEIRO, Stella Sueli Lourenço BRAGA, Karla ZANCOPÉ, Richard Bengt PRICE, Carlos José SOARES
The thickness and shade of a restoration will affect the transmission of light from the light-curing unit (LCU). This study determined the power (mW), spectral radiant power (mW/nm), and beam profile of different LCUs through various thicknesses and shades of a CAD-CAM resin composite (BRAVA Block, FGM). Five thicknesses: 0.5; 0.75; 1.0; 1.5, and 2.0 mm, in three shades: Bleach; A2 and A3.5 of a CAD-CAM resin (n = 5). Two single-peak LCUs: EL, Elipar DeepCure-S (3M Oral Care); and OP, Optilight Max (Gnatus), and one multiple-peak LCU: VL, VALO Grand (Ultradent), were used. The LCUs were positioned touching the surface of the BRAVA Block. The power and emission spectrum were measured using a fiberoptic spectrometer attached to an integrating sphere, and the beam profiles using a laser beam profiler. The effect of the material thickness on the light attenuation coefficients was determined. VL and EL delivered more homogeneous beam profiles than OP. The type of the BRAVA Block had a significant effect on the transmitted power, and wavelengths of transmitted light (p < 0.001). There was an exponential reduction in the power and emission spectrum as the thickness of the BRAVA Block increased (p < 0.001). The light transmission through the A2 shade was least affected by the thickness (p < 0.001). The attenuation coefficient was higher for the violet light and higher for A3.5 than the A2 or Bleach shades. No violet light from the VL could be detected at the bottom of 2.0 mm of the BRAVA Block.
修复物的厚度和阴影会影响光固化单元(LCU)的光透射。本研究通过不同厚度和颜色的CAD-CAM树脂复合材料(BRAVA Block, FGM)测定了不同lcu的功率(mW)、光谱辐射功率(mW/nm)和光束轮廓。五种厚度:0.5;0.75;1.0;1.5和2.0毫米,有三种颜色:漂白;A2和A3.5的CAD-CAM树脂(n = 5)。两个单峰lcu: EL, Elipar DeepCure-S (3M Oral Care);和OP, Optilight Max (Gnatus),以及一个多峰LCU: VL, VALO Grand (Ultradent)。lcu放置于BRAVA块的表面。功率和发射光谱的测量采用了连接在积分球上的光纤光谱仪,光束轮廓采用了激光光束轮廓仪。确定了材料厚度对光衰减系数的影响。与op相比,VL和EL的光束轮廓更均匀。BRAVA块的类型对透射功率和透射光波长有显著影响(p < 0.001)。随着BRAVA块厚度的增加,功率和发射光谱呈指数降低(p < 0.001)。A2遮光片厚度对透光率的影响最小(p < 0.001)。紫光和A3.5光的衰减系数高于A2或漂白光。BRAVA块底部2.0 mm处未检测到VL紫光。
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引用次数: 0
Glycogen synthase kinase 3 inhibition enhances mineral nodule formation by cementoblasts in vitro 糖原合成酶激酶3抑制促进骨水泥母细胞形成矿物结节
4区 医学 Q2 Dentistry Pub Date : 2023-01-01 DOI: 10.1590/1807-3107bor-2023.vol37.0112
Henrique ABDALLA, Rafael STORINO, Amanda BANDEIRA, Lucas TEIXEIRA, Ana MILLÁS, Paulo LISBOA-FILHO, Kamila KANTOVITZ, Francisco NOCITI JUNIOR
This study aimed to investigate whether GSK-3 inhibition (CHIR99021) effectively promoted mineralization by cementoblasts (OCCM-30). OCCM-30 cells were used and treated with different concentrations of CHIR99021 (2.5, 5, and 10 mM). Experiments included proliferation and viability, cellular metabolic activity, gene expression, and mineral nodule formation by Xylene Orange at the experimental time points. In general, CHIR99021 did not significantly affect OCCM-30 viability and cell metabolism (MTT assay) (p > 0.05), but increased OCCM-30 proliferation at 2.5 mM on days 2 and 4 (p < 0.05). Data analysis further showed that inhibition of GSK-3 resulted in increased transcript levels of Axin2 in OCCM-30 cells starting as early as 4 h, and regulated the expression of key bone markers including alkaline phosphatase (Alp), runt-related transcription factor 2 (Runx-2), osteocalcin (Ocn), and osterix (Osx). In addition, CHIR99021 led to an enhanced mineral nodule formation in vitro under both osteogenic and non-osteogenic conditions as early as 5 days after treatment. Altogether, the results of the current study suggest that inhibition of GSK-3 has the potential to promote cementoblast differentiation leading to increased mineral deposition in vitro.
本研究旨在探讨GSK-3抑制剂(CHIR99021)是否能有效促进成水泥细胞(OCCM-30)矿化。用不同浓度的CHIR99021(2.5、5和10 mM)处理OCCM-30细胞。实验包括二甲苯橙在实验时间点的增殖和活力、细胞代谢活性、基因表达和矿物质结节形成。总体而言,CHIR99021对OCCM-30细胞活力和细胞代谢(MTT法)无显著影响(p > 0.05),但在第2天和第4天增加了2.5 mM OCCM-30的增殖(p < 0.05)。数据分析进一步表明,抑制GSK-3可导致OCCM-30细胞中Axin2转录水平早在4 h就开始升高,并调节碱性磷酸酶(Alp)、矮子相关转录因子2 (Runx-2)、骨钙素(Ocn)和骨甾体(Osx)等关键骨标志物的表达。此外,CHIR99021早在治疗后5天就在体外促进了成骨和非成骨条件下的矿物结节形成。总之,目前的研究结果表明,抑制GSK-3有可能促进成水泥细胞分化,导致体外矿物质沉积增加。
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引用次数: 0
Effect of modeling liquids on resin composite roughness and color parameters after staining and toothbrushing. 造型液对染色和刷牙后树脂复合材料粗糙度和颜色参数的影响。
IF 2.5 4区 医学 Q2 Dentistry Pub Date : 2023-01-01 DOI: 10.1590/1807-3107bor-2023.vol37.0024
Thaís Souza Maia, Thamires Diogo Lima, Vanessa Miranda Ramos, André Luís Faria-E-Silva, Murilo de Sousa Menezes

This study evaluated surface roughness, color stability, whitening index, and opacity of different types of modeling liquids for resin composite coating after exposure to staining and toothbrushing. Disc-shaped resin composite (Vittra APS, FGM) specimens were fabricated and divided into four groups (n = 10 each): control group, Composite Wetting resin (Ultradent Products), Adper Scotchbond Multipurpose adhesive (3M ESPE), and Adper Universal adhesive (3M ESPE). Surface roughness (Ra) was measured using a rugosimeter, while color stability (∆E00), whitening index (WI), and opacity (%) were measured using a spectrophotometer. Assessments were made at four time points: after polishing (baseline, T1), after immersion in red wine for 24 h (T2), and after 5,000 (T3) and 10,000 (T4) cycles of toothbrushing. Scanning electron microscopy images were captured to analyze the scratches created. The data were statistically analyzed by two-way repeated-measures analysis of variance and Tukey's honestly significant difference tests (α = .05). Modeling with the Wetting resin resulted in higher surface roughness (p < 0.05) and low color stability, which were attributable to porosities. Higher color change values were observed in the control group after staining. Both adhesives showed the lowest mean ΔE00 values (p < 0.005). WI decreased after staining, except with the use of the Universal adhesive (p < 0.005). The lowest opacity values were observed at baseline for all groups (p < 0.005). The Universal and Scotchbond adhesives had lower surface roughness, better color stability, higher WI, and the lowest opacity values after staining with red wine and toothbrushing.

本研究评估了不同类型树脂复合涂层在染色和刷牙后的表面粗糙度、颜色稳定性、美白指数和不透明度。制作盘状树脂复合材料(Vittra APS, FGM)标本,分为4组(每组10个):对照组、复合润湿树脂(Ultradent Products)、Adper Scotchbond多用途粘合剂(3M ESPE)和Adper通用粘合剂(3M ESPE)。表面粗糙度(Ra)用rugosimeter测量,颜色稳定性(∆E00)、美白指数(WI)、不透明度(%)用分光光度计测量。在四个时间点进行评估:抛光后(基线,T1),浸泡在红酒中24小时后(T2),刷牙5000次(T3)和10000次(T4)循环后。扫描电子显微镜图像被捕获来分析产生的划痕。采用双向重复测量方差分析和Tukey's诚实显著性差异检验(α = 0.05)对数据进行统计学分析。使用湿性树脂建模导致表面粗糙度较高(p < 0.05),颜色稳定性较低,这是由于孔隙造成的。对照组染色后颜色变化值较高。两种粘接剂的平均ΔE00值最低(p < 0.005)。除使用万能胶外,染色后WI降低(p < 0.005)。所有组在基线时观察到最低的不透明度值(p < 0.005)。用红酒和牙刷染色后,Universal和Scotchbond胶粘剂的表面粗糙度较低,颜色稳定性较好,WI较高,不透明度最低。
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引用次数: 0
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Brazilian Oral Research
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