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Craniofacial morphology of patients with unilateral cleft lip and palate at two stages of skeletal maturation. 单侧唇腭裂患者骨骼成熟两个阶段的颅面形态。
IF 2.5 4区 医学 Q2 Dentistry Pub Date : 2023-01-01 DOI: 10.1590/1807-3107bor-2023.vol37.0009
Eduardo Murad Villoria, Bernardo Quiroga Souki, Flávio Lucena Antunes, Marina Araújo Leite Assis, Ildeu Andrade Júnior, Dauro Douglas Oliveira, Rodrigo Villamarim Soares

The aim of this retrospective cross-sectional investigation was to perform a 3D analysis of craniofacial morphology of patients with unilateral cleft lip and palate (UCLP) at two stages of skeletal maturation. Cone-beam computed tomography (CBCT) scans of 52 UCLP patients (34 prepubertal; 18 pubertal) were collected from an outpatient referral center for the treatment of craniofacial deformities. In total 15 multiplanar craniofacial landmarks were identified, 3D virtual surface models were created, and 13 variables were measured to assess the 3D Euclidean distances between landmarks and spatial position of the landmarks in the projected X, Y and Z components. Maxillary and mandibular pitch (clockwise, counterclockwise) rotation relative to the cranial base was also evaluated. The significance level was set at 5%. Maxillary retrusion value relative to the cranial base was higher and statistically significant greater (p = 0.028) in pubertal (SNA, 77.4° ± 6.2; N-ANS Y, 3.3 mm ± 3.1) than in prepubertal patients (SNA 81.0° ± 5.2; N-ANS Y, 5.8 mm ± 2.7). The posterior cranial base length (S-Ba Y) was significantly longer (p = 0.013) in pubertal (20.7 mm ± 3.4) than in prepubertal patients (18.4 mm ± 2.7). The upper facial height (N-ANS Z) was significantly greater (p = 0.01) in pubertal (46.9 mm ± 4.5) than in prepubertal patients (43.4 mm ± 3.0). Prepubertal and pubertal UCLP patients presented distinct patterns of craniofacial morphology, mainly in the sagittal component of the maxilla and in the posterior cranial base length. Pubertal patients had greater maxillary retrusion and posterior cranial base length.

本回顾性横断面研究的目的是在骨骼成熟的两个阶段对单侧唇腭裂(UCLP)患者的颅面形态进行三维分析。锥形束ct (CBCT)扫描52例UCLP患者(34例青春期前;从门诊转诊中心收集治疗颅面畸形的患者。总共识别了15个多平面颅面地标,创建了三维虚拟表面模型,并测量了13个变量来评估地标之间的三维欧几里得距离以及地标在投影X, Y和Z分量中的空间位置。还评估了上颌和下颌相对于颅底的螺距(顺时针、逆时针)旋转。显著性水平设为5%。上颌后缩值相对于颅底较高,青春期(SNA, 77.4°±6.2)差异有统计学意义(p = 0.028);N-ANS Y, 3.3 mm±3.1)高于青春期前患者(SNA 81.0°±5.2;N-ANS Y, 5.8 mm±2.7)。青春期患者后颅底长度(S-Ba - Y) (20.7 mm±3.4)明显长于青春期前患者(18.4 mm±2.7)(p = 0.013)。青春期患者上面部高度(N-ANS - Z) (46.9 mm±4.5)显著高于青春期前患者(43.4 mm±3.0),差异有统计学意义(p = 0.01)。青春期前和青春期UCLP患者的颅面形态不同,主要表现在上颌骨矢状面部分和后颅底长度。青春期患者上颌后缩较大,后颅底长度较大。
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引用次数: 0
Dental visits and depression mediating the association of socioeconomic status with oral health behaviors. 社会经济地位与口腔健康行为的关系:看牙和抑郁。
IF 2.5 4区 医学 Q2 Dentistry Pub Date : 2023-01-01 DOI: 10.1590/1807-3107bor-2022.vol36.0094
Orlando Luiz do Amaral Júnior, Maria Laura Braccini Fagundes, Lucelen Fontoura Bastos, Gabriele Rissotto Menegazzo, Fernando Neves Hugo, Lucas Guimarães Abreu, Betine Pinto Moehlecke Iser, Juliana Balbinot Hilgert, Jessye Melgarejo do Amaral Giordani

Determinants of oral diseases include behaviors, which in turn are influenced by a series of social determinants such as psychosocial aspects and dental care services. Therefore, the aim of this study was to investigate whether depressive symptoms and use of dental care services mediate the relationship between socioeconomic status (SES) and oral health behaviors. This was a cross-sectional study that analyzed data from participants in the 2019 National Health Survey (PNS) (n = 88,531). The eligibility criteria were individuals who were 18 years and older and exclusion criterion was living in households located in special or sparsely populated census tracts. Structural equation modeling (SEM) was used to test direct and indirect pathways from a latent variable for SES to oral health through depressive symptoms (assessed through the "Patient Health Questionnaire-9") and use of dental care services. The maximum likelihood estimator for complex samples with the robust standard error was used. The final model presented an adequate fit: RMESA of 0.008, CFI of 0.998, and SMRM of 0.005. The results showed that higher SES was directly associated with better oral health-related behaviors [standardized coefficient (SC): 0.428; p < 0.01] and indirectly through depressive symptoms [(SC): 0.002; p < 0.01] and dental care services [(SC): 0.089; p < 0.01]. The total effect of SES on oral health-related behaviors was equal to [(SC: 0.519 (p < 0.01)]. In conclusion, the findings suggest that high socioeconomic status, mediated by depressive symptoms and dental care services, has a positive effect on oral health.

口腔疾病的决定因素包括行为,而行为又受到一系列社会决定因素的影响,如心理社会方面和牙科保健服务。因此,本研究的目的是探讨抑郁症状和牙科保健服务的使用是否介导社会经济地位(SES)与口腔健康行为之间的关系。这是一项横断面研究,分析了2019年全国健康调查(PNS)参与者的数据(n = 88,531)。资格标准是年满18岁的个人,排除标准是居住在特殊或人口稀少的人口普查区的家庭。使用结构方程模型(SEM)来测试SES潜在变量通过抑郁症状(通过“患者健康问卷-9”进行评估)和使用牙科保健服务对口腔健康的直接和间接途径。对具有鲁棒标准误差的复杂样本采用极大似然估计。最终模型拟合良好:RMESA为0.008,CFI为0.998,SMRM为0.005。结果表明,较高的社会经济地位与较好的口腔健康相关行为直接相关[标准化系数(SC): 0.428;p < 0.01]与抑郁症状间接相关[(SC): 0.002;p < 0.01]口腔保健服务[(SC): 0.089;P < 0.01]。SES对口腔健康行为的总影响为[(SC: 0.519 (p < 0.01)]。综上所述,高社会经济地位,由抑郁症状和牙科保健服务介导,对口腔健康有积极影响。
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引用次数: 0
Assessment of the contextual effects on the prevalence of periodontitis: a systematic review. 评估对牙周炎流行的环境影响:一项系统综述。
IF 2.5 4区 医学 Q2 Dentistry Pub Date : 2023-01-01 DOI: 10.1590/1807-3107bor-2022.vol36.0125
Leonardo Vilar Filgueiras, Willian Konflanz, Alex Nogueira Haas, Roger Keller Celeste

This study reviews the influence of contextual factors on periodontitis based on a systematic search of studies recorded in the PubMed, Scopus, Web of Science, and EMBASE databases. Periodontitis was assessed by clinical attachment loss and probing depth for studies with data on the socioeconomic status (SES) of a specific area (area-level SES) or dental care service (service-level) in a catchment area among individuals aged 18 and over. Two independent reviewers performed study selection, data extraction, and assessment of methodological quality. Of the 646 articles identified, 13 were included in the systematic review, representing 101,362 individuals from five countries (USA, UK, Brazil, China, and Uruguay). A higher prevalence of periodontitis was described in lower SES neighborhoods, more deprived postcodes, and poorer provinces. Gini Index results were mixed and inconclusive. Three studies showed that higher coverage of primary dental care at the municipal level was associated with a lower prevalence of periodontitis. Contextual factors at the area-level SES and dental care service might influence periodontitis, but the existing evidence is unclear. The contextual effect is important for periodontal health and may contribute to the prevalence of periodontitis, independent of well-known risk factors and individual levels.

本研究通过对PubMed、Scopus、Web of Science和EMBASE数据库中记录的研究进行系统检索,回顾了环境因素对牙周炎的影响。牙周炎是通过临床附着丧失和探测深度来评估的,研究数据包括特定区域(区域级SES)的社会经济地位(SES)或集水区18岁及以上个体的牙科保健服务(服务水平)。两名独立审稿人进行了研究选择、数据提取和方法学质量评估。在确定的646篇文章中,13篇被纳入系统评价,代表来自5个国家(美国、英国、巴西、中国和乌拉圭)的101362人。在社会经济地位较低的社区、较贫困的邮政编码和较贫穷的省份,牙周炎的患病率较高。基尼指数的结果好坏参半,尚无定论。三项研究表明,市政一级初级牙科保健覆盖率较高与牙周炎患病率较低有关。背景因素在地区水平的SES和牙科保健服务可能影响牙周炎,但现有的证据尚不清楚。环境影响对牙周健康很重要,并可能导致牙周炎的流行,独立于已知的危险因素和个人水平。
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引用次数: 1
Difficulties of dental practice among left-handed Tunisian students: a cross-sectional survey. 突尼斯左撇子学生牙科治疗的困难:一项横断面调查。
IF 2.5 4区 医学 Q2 Dentistry Pub Date : 2023-01-01 DOI: 10.1590/1807-3107bor-2023.vol37.0032
Emna Hidoussi Sakly, Alessandro Leite Cavalcanti, Oumayma Nasr

In dentistry, most equipment is designed for right-handed (RH) individuals. Thus, left-handed (LH) individuals are often forced to adapt to demanding RH working conditions, and therefore, experience difficulties in their practice. The objective of this study was to determine the prevalence of left handedness among dental students at the Dental Clinic of Monastir, Tunisia, and to investigate the difficulties for LH individuals during clinical practice. A cross-sectional study was conducted among dental students during the academic year from September 2019 to March 2020. An adaptation of the Grad-Corllet Diagram and a specific questionnaire on clinical practices were administered to 221 participants. Data were analyzed using SPSS 24.0 statistical software for descriptive statistics and the chi-square test was performed (with a 5% significance level). The study revealed that 18.1% of dental students were LH. Among which, 82.5% of LH students had difficulties using instruments designed for RH dentists, 47.5% of LH students preferred the 3 o'clock working position, and 77.5% of LH students preferred to work in a sitting position. Most LH students (70%) reported that endodontic treatment was the most difficult procedure to perform. All students, whether RH or LH, presented a higher percentage of pain in the lumbar and neck/cervical regions; however, LH students presented higher values (77.5%) with statistically significant differences being reported for lower back pain (p = 0.026) and neck pain (p = 0.012). This study highlights the difficulties that LH dental students face in performing dental work. Dental Schools should provide LH students with appropriate equipment and a proper learning environment.

在牙科中,大多数设备是为右撇子(RH)设计的。因此,左撇子(LH)个体经常被迫适应苛刻的RH工作条件,因此,在他们的实践中遇到困难。本研究的目的是确定在突尼斯Monastir牙科诊所的牙科学生中左撇子的患病率,并调查左右手个体在临床实践中的困难。在2019年9月至2020年3月的学年期间,对牙科学生进行了一项横断面研究。根据Grad-Corllet图和一份关于临床实践的具体问卷对221名参与者进行了调整。数据分析采用SPSS 24.0统计软件进行描述性统计,采用卡方检验(显著性水平为5%)。研究显示,18.1%的牙科学生为LH。其中,82.5%的LH学生使用为RH牙医设计的器械有困难,47.5%的LH学生喜欢3点工作姿势,77.5%的LH学生喜欢坐着工作。大多数LH学生(70%)报告说根管治疗是最困难的手术。所有的学生,无论是RH还是LH,在腰椎和颈部/颈椎区域表现出更高的疼痛百分比;然而,LH学生表现出更高的数值(77.5%),腰痛(p = 0.026)和颈痛(p = 0.012)的差异有统计学意义。本研究强调了LH牙科学生在进行牙科工作时所面临的困难。牙科学校应为LH学生提供适当的设备和适当的学习环境。
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引用次数: 1
Students' perception to an interactive web-based response system in oral and maxillofacial pathology teaching. 学生对口腔颌面病理学教学中互动式网路回应系统的感受。
IF 2.5 4区 医学 Q2 Dentistry Pub Date : 2023-01-01 DOI: 10.1590/1807-3107bor-2023.vol37.0027
Luana Rafaela Garcez da Silva, Hélen Kaline Farias Bezerra, Augusto César Leal da Silva Leonel, Elaine Judite de Amorim Carvalho, Jurema Freire Lisboa de Castro, Alan Roger Santos-Silva, Danyel Elias da Cruz Perez

This study evaluated dental students' perceptions and adherence to an interactive web-based response system in the teaching of Oral and Maxillofacial Pathology. Between 2018 and 2019, students from a single Brazilian dental school used the Poll Everywhere® app to answer questions on subjects taught during an Oral and Maxillofacial Pathology course. At the end of the academic semester, the students completed a questionnaire containing 10 questions regarding the app's use. The study included 123 students. Regarding the devices used to answer the questions on the app, 117 (95.1%) students used a smartphone and 3 (2.4%) used a laptop. Almost all students (121; 98.4%) agreed that this interactive web-based response system provided the teacher with a better overview of students' understanding and improved their self-assessment of the acquired knowledge of the subjects. Most students (118; 95.9%) preferred classes using this technology and 122 (99.2%) stated that using the app made them feel more engaged in classes. In addition, all students agreed that the app improved student-teacher interactions. Most students (119; 96.7%) considered the digital interactive method more attractive than the conventional teaching approach, and 99 (80.5%) did not have any negative comments regarding the app. In conclusion, the Poll Everywhere® app provides a more dynamic and attractive educational environment for Oral and Maxillofacial Pathology teaching.

本研究评估了牙科学生在口腔颌面病理学教学中对交互式网络反应系统的认知和依从性。在2018年至2019年期间,来自一所巴西牙科学校的学生使用Poll Everywhere®应用程序回答有关口腔颌面病理学课程中教授的科目的问题。在学期结束时,学生们完成了一份调查问卷,其中包含10个关于应用程序使用的问题。这项研究包括123名学生。117名(95.1%)学生使用智能手机,3名(2.4%)学生使用笔记本电脑。几乎所有的学生(121;98.4%)同意这种基于网络的互动回应系统可以让教师更好地了解学生的理解情况,并提高他们对所学学科知识的自我评估。大多数学生(118人;95.9%的学生更喜欢使用这种技术的课程,122名学生(99.2%)表示,使用该应用程序让他们在课堂上更投入。此外,所有学生都认为该应用程序改善了师生互动。大多数学生(119人;96.7%的学生认为数字化互动教学方式比传统教学方式更具吸引力,99名学生(80.5%)对应用程序没有任何负面评价。综上所述,Poll Everywhere®应用程序为口腔颌面病理学教学提供了一个更有活力和吸引力的教育环境。
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引用次数: 0
Longitudinal study for dental caries calibration of dentists unexperienced in epidemiological surveys. 无流行病学调查经验牙医龋齿校正的纵向研究。
IF 2.5 4区 医学 Q2 Dentistry Pub Date : 2023-01-01 DOI: 10.1590/1807-3107bor-2023.vol37.0023
Mariana Nabarrette, Patrícia Rafaela Dos Santos, Andréa Videira Assaf, Glaucia Maria Bovi Ambrosano, Marcelo de Castro Meneghim, Silvia Amélia Scudeler Vedovello, Karine Laura Cortellazzi

This study aimed to make a longitudinal analysis of interexaminer calibration reproducibility in diagnosing dental caries in posterior teeth, by examiners without previous experience in epidemiological studies. A group of 11 inexperienced examiners underwent theoretical-practical training and calibration assessments, assisted by a standard examiner. An examiner who did not participate directly in the research selected 5-year-old children with and without caries. The D3 diagnostic threshold was used to evaluate dental caries, based on the World Health Organization (WHO) criteria. The initial calibration (baseline) was performed after the theoretical-practical training session, and consisted of examining 20 children; the second calibration occurred three months later, and involved evaluating another 18 children. The interexaminer agreement was obtained by kappa statistics, and by overall percentage agreement. The paired t-test was applied to compare the values for kappa means and overall percentage agreement between the time points studied. At baseline, the values for kappa (> 0.81) and overall percentage agreement (> 95.63%) were considered high. At the 3-month calibration assessment, all the examiners showed some decrease in both kappa (p < 0.0001) and overall percentage agreement (p = 0.0102). The calibration process currently proposed by the WHO is effective. However, reproducibility was not maintained over time for inexperienced examiners evaluating the posterior teeth of 5-year-old children, under epidemiological conditions.

本研究旨在对没有流行病学研究经验的检验员在诊断后牙龋齿时的校准可重复性进行纵向分析。由11名没有经验的审查员组成的小组在一名标准审查员的协助下接受了理论实践培训和校准评估。一位没有直接参与研究的考官选择了有和没有龋齿的5岁儿童。根据世界卫生组织(WHO)的标准,使用D3诊断阈值来评估龋齿。初始校准(基线)在理论-实践培训课程结束后进行,包括检查20名儿童;第二次校准发生在三个月后,涉及评估另外18名儿童。通过kappa统计和总体百分比一致获得审查员之间的一致。配对t检验用于比较kappa平均值和研究时间点之间总体百分比一致性的值。在基线时,kappa值(> 0.81)和总体百分比一致性(> 95.63%)被认为是高的。在3个月的校准评估中,所有的审查员都显示kappa (p < 0.0001)和总体百分比一致性(p = 0.0102)有所下降。世卫组织目前提出的校准过程是有效的。然而,随着时间的推移,在流行病学条件下,没有经验的检查员评估5岁儿童的后牙时,不能保持再现性。
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引用次数: 0
Sense of coherence and oral health-related quality of life among southern Brazilian male adolescents 巴西南部男性青少年的连贯感和口腔健康相关生活质量
4区 医学 Q2 Dentistry Pub Date : 2023-01-01 DOI: 10.1590/1807-3107bor-2023.vol37.0113
Nicássia Cioquetta LOCK, Maria Laura Castro Alves Ribeiro GAZOLA, Patrícia Kolling MARQUEZAN, Julio Eduardo do Amaral ZENKNER, Luana Severo ALVES
This study evaluated the association between sense of coherence (SoC) and oral health-related quality of life (OHRQoL) among conscripts of the Brazilian Army, in two cities of southern Brazil. A cross-sectional study included all 18-19-year-old adolescents who joined the Brazilian Army as draftees for mandatory military service in the cities of Itaqui, RS, and Santiago, RS (n = 505). Data collection was conducted from 2019 to 2021, and included the application of questionnaires and a clinical oral examination to record gingivitis, malocclusion, and dental caries. OHRQoL was collected through the Brazilian short version of the Oral Health Impact Profile (OHIP), composed of 14 questions. The adolescents’ SoC was assessed using the validated Brazilian version of the SOC-13 scale. The primary outcome of this study was OHRQoL, modeled as a discrete variable (OHIP-14 scores). The main predictor variable was SoC, categorized as low, moderate, or high. The association between predictor variables and OHRQoL was assessed by Poisson regression models using a hierarchical approach. Unadjusted and adjusted rate ratios (RR), and 95% confidence intervals (CI) were estimated. All the analyses were performed using STATA software version 14.2. Adolescents with a moderate and high SoC had 27% (RR = 0.73, 95%CI = 0.64–0.84) and 51% (RR = 0.49, 95%CI = 0.41–0.58) lower mean OHIP-14 scores, respectively, than those with a low SoC score, after the inclusion of behavioral and clinical variables. This study showed a significant association between SoC and OHRQoL among 18–19-year-old southern Brazilian adolescents. Strengthening the SoC as a psychosocial resource may improve the well-being and OHRQoL of adolescents.
本研究评估了巴西南部两个城市巴西军队应征入伍者的连贯感(SoC)与口腔健康相关生活质量(OHRQoL)之间的关系。横断面研究纳入了巴西伊塔基市和圣地亚哥市所有18-19岁的青少年,这些青少年作为义务兵役的应征入伍者加入了巴西军队(n = 505)。数据收集于2019年至2021年进行,包括使用问卷调查和临床口腔检查,记录牙龈炎、错颌合和龋齿。OHRQoL是通过巴西口腔健康影响概况(OHIP)的简短版本收集的,由14个问题组成。使用经过验证的巴西版SoC -13量表对青少年的SoC进行评估。本研究的主要结局是OHRQoL,建模为离散变量(OHIP-14评分)。主要预测变量是SoC,分为低、中、高三个等级。预测变量与OHRQoL之间的关系通过泊松回归模型采用分层方法进行评估。估计未调整和调整的率比(RR)和95%置信区间(CI)。所有分析均使用STATA软件14.2版进行。在纳入行为和临床变量后,中度和高SoC青少年的平均OHIP-14得分分别比低SoC青少年低27% (RR = 0.73, 95%CI = 0.64-0.84)和51% (RR = 0.49, 95%CI = 0.41-0.58)。本研究显示18 - 19岁巴西南部青少年SoC与OHRQoL之间存在显著相关性。加强社会归属感作为一种社会心理资源可以改善青少年的幸福感和OHRQoL。
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引用次数: 0
Effect of thickness and shade of CAD/CAM composite on the light transmission from different light-curing units CAD/CAM复合材料厚度和色度对不同光固化单元透光性的影响
4区 医学 Q2 Dentistry Pub Date : 2023-01-01 DOI: 10.1590/1807-3107bor-2023.vol37.0114
Julia Dantas MAZÃO, Maria Tereza Hordones RIBEIRO, Stella Sueli Lourenço BRAGA, Karla ZANCOPÉ, Richard Bengt PRICE, Carlos José SOARES
The thickness and shade of a restoration will affect the transmission of light from the light-curing unit (LCU). This study determined the power (mW), spectral radiant power (mW/nm), and beam profile of different LCUs through various thicknesses and shades of a CAD-CAM resin composite (BRAVA Block, FGM). Five thicknesses: 0.5; 0.75; 1.0; 1.5, and 2.0 mm, in three shades: Bleach; A2 and A3.5 of a CAD-CAM resin (n = 5). Two single-peak LCUs: EL, Elipar DeepCure-S (3M Oral Care); and OP, Optilight Max (Gnatus), and one multiple-peak LCU: VL, VALO Grand (Ultradent), were used. The LCUs were positioned touching the surface of the BRAVA Block. The power and emission spectrum were measured using a fiberoptic spectrometer attached to an integrating sphere, and the beam profiles using a laser beam profiler. The effect of the material thickness on the light attenuation coefficients was determined. VL and EL delivered more homogeneous beam profiles than OP. The type of the BRAVA Block had a significant effect on the transmitted power, and wavelengths of transmitted light (p < 0.001). There was an exponential reduction in the power and emission spectrum as the thickness of the BRAVA Block increased (p < 0.001). The light transmission through the A2 shade was least affected by the thickness (p < 0.001). The attenuation coefficient was higher for the violet light and higher for A3.5 than the A2 or Bleach shades. No violet light from the VL could be detected at the bottom of 2.0 mm of the BRAVA Block.
修复物的厚度和阴影会影响光固化单元(LCU)的光透射。本研究通过不同厚度和颜色的CAD-CAM树脂复合材料(BRAVA Block, FGM)测定了不同lcu的功率(mW)、光谱辐射功率(mW/nm)和光束轮廓。五种厚度:0.5;0.75;1.0;1.5和2.0毫米,有三种颜色:漂白;A2和A3.5的CAD-CAM树脂(n = 5)。两个单峰lcu: EL, Elipar DeepCure-S (3M Oral Care);和OP, Optilight Max (Gnatus),以及一个多峰LCU: VL, VALO Grand (Ultradent)。lcu放置于BRAVA块的表面。功率和发射光谱的测量采用了连接在积分球上的光纤光谱仪,光束轮廓采用了激光光束轮廓仪。确定了材料厚度对光衰减系数的影响。与op相比,VL和EL的光束轮廓更均匀。BRAVA块的类型对透射功率和透射光波长有显著影响(p < 0.001)。随着BRAVA块厚度的增加,功率和发射光谱呈指数降低(p < 0.001)。A2遮光片厚度对透光率的影响最小(p < 0.001)。紫光和A3.5光的衰减系数高于A2或漂白光。BRAVA块底部2.0 mm处未检测到VL紫光。
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引用次数: 0
Glycogen synthase kinase 3 inhibition enhances mineral nodule formation by cementoblasts in vitro 糖原合成酶激酶3抑制促进骨水泥母细胞形成矿物结节
4区 医学 Q2 Dentistry Pub Date : 2023-01-01 DOI: 10.1590/1807-3107bor-2023.vol37.0112
Henrique ABDALLA, Rafael STORINO, Amanda BANDEIRA, Lucas TEIXEIRA, Ana MILLÁS, Paulo LISBOA-FILHO, Kamila KANTOVITZ, Francisco NOCITI JUNIOR
This study aimed to investigate whether GSK-3 inhibition (CHIR99021) effectively promoted mineralization by cementoblasts (OCCM-30). OCCM-30 cells were used and treated with different concentrations of CHIR99021 (2.5, 5, and 10 mM). Experiments included proliferation and viability, cellular metabolic activity, gene expression, and mineral nodule formation by Xylene Orange at the experimental time points. In general, CHIR99021 did not significantly affect OCCM-30 viability and cell metabolism (MTT assay) (p > 0.05), but increased OCCM-30 proliferation at 2.5 mM on days 2 and 4 (p < 0.05). Data analysis further showed that inhibition of GSK-3 resulted in increased transcript levels of Axin2 in OCCM-30 cells starting as early as 4 h, and regulated the expression of key bone markers including alkaline phosphatase (Alp), runt-related transcription factor 2 (Runx-2), osteocalcin (Ocn), and osterix (Osx). In addition, CHIR99021 led to an enhanced mineral nodule formation in vitro under both osteogenic and non-osteogenic conditions as early as 5 days after treatment. Altogether, the results of the current study suggest that inhibition of GSK-3 has the potential to promote cementoblast differentiation leading to increased mineral deposition in vitro.
本研究旨在探讨GSK-3抑制剂(CHIR99021)是否能有效促进成水泥细胞(OCCM-30)矿化。用不同浓度的CHIR99021(2.5、5和10 mM)处理OCCM-30细胞。实验包括二甲苯橙在实验时间点的增殖和活力、细胞代谢活性、基因表达和矿物质结节形成。总体而言,CHIR99021对OCCM-30细胞活力和细胞代谢(MTT法)无显著影响(p > 0.05),但在第2天和第4天增加了2.5 mM OCCM-30的增殖(p < 0.05)。数据分析进一步表明,抑制GSK-3可导致OCCM-30细胞中Axin2转录水平早在4 h就开始升高,并调节碱性磷酸酶(Alp)、矮子相关转录因子2 (Runx-2)、骨钙素(Ocn)和骨甾体(Osx)等关键骨标志物的表达。此外,CHIR99021早在治疗后5天就在体外促进了成骨和非成骨条件下的矿物结节形成。总之,目前的研究结果表明,抑制GSK-3有可能促进成水泥细胞分化,导致体外矿物质沉积增加。
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引用次数: 0
Effect of modeling liquids on resin composite roughness and color parameters after staining and toothbrushing. 造型液对染色和刷牙后树脂复合材料粗糙度和颜色参数的影响。
IF 2.5 4区 医学 Q2 Dentistry Pub Date : 2023-01-01 DOI: 10.1590/1807-3107bor-2023.vol37.0024
Thaís Souza Maia, Thamires Diogo Lima, Vanessa Miranda Ramos, André Luís Faria-E-Silva, Murilo de Sousa Menezes

This study evaluated surface roughness, color stability, whitening index, and opacity of different types of modeling liquids for resin composite coating after exposure to staining and toothbrushing. Disc-shaped resin composite (Vittra APS, FGM) specimens were fabricated and divided into four groups (n = 10 each): control group, Composite Wetting resin (Ultradent Products), Adper Scotchbond Multipurpose adhesive (3M ESPE), and Adper Universal adhesive (3M ESPE). Surface roughness (Ra) was measured using a rugosimeter, while color stability (∆E00), whitening index (WI), and opacity (%) were measured using a spectrophotometer. Assessments were made at four time points: after polishing (baseline, T1), after immersion in red wine for 24 h (T2), and after 5,000 (T3) and 10,000 (T4) cycles of toothbrushing. Scanning electron microscopy images were captured to analyze the scratches created. The data were statistically analyzed by two-way repeated-measures analysis of variance and Tukey's honestly significant difference tests (α = .05). Modeling with the Wetting resin resulted in higher surface roughness (p < 0.05) and low color stability, which were attributable to porosities. Higher color change values were observed in the control group after staining. Both adhesives showed the lowest mean ΔE00 values (p < 0.005). WI decreased after staining, except with the use of the Universal adhesive (p < 0.005). The lowest opacity values were observed at baseline for all groups (p < 0.005). The Universal and Scotchbond adhesives had lower surface roughness, better color stability, higher WI, and the lowest opacity values after staining with red wine and toothbrushing.

本研究评估了不同类型树脂复合涂层在染色和刷牙后的表面粗糙度、颜色稳定性、美白指数和不透明度。制作盘状树脂复合材料(Vittra APS, FGM)标本,分为4组(每组10个):对照组、复合润湿树脂(Ultradent Products)、Adper Scotchbond多用途粘合剂(3M ESPE)和Adper通用粘合剂(3M ESPE)。表面粗糙度(Ra)用rugosimeter测量,颜色稳定性(∆E00)、美白指数(WI)、不透明度(%)用分光光度计测量。在四个时间点进行评估:抛光后(基线,T1),浸泡在红酒中24小时后(T2),刷牙5000次(T3)和10000次(T4)循环后。扫描电子显微镜图像被捕获来分析产生的划痕。采用双向重复测量方差分析和Tukey's诚实显著性差异检验(α = 0.05)对数据进行统计学分析。使用湿性树脂建模导致表面粗糙度较高(p < 0.05),颜色稳定性较低,这是由于孔隙造成的。对照组染色后颜色变化值较高。两种粘接剂的平均ΔE00值最低(p < 0.005)。除使用万能胶外,染色后WI降低(p < 0.005)。所有组在基线时观察到最低的不透明度值(p < 0.005)。用红酒和牙刷染色后,Universal和Scotchbond胶粘剂的表面粗糙度较低,颜色稳定性较好,WI较高,不透明度最低。
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Brazilian Oral Research
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