Cytojournal最新文献

Objectives: Herein, we present the PancreaSeq® results of 28 patients and emphasize the usefulness of molecular testing in evaluation of pancreatic cysts.

Material and methods: A total of 10 (35.7%) non-diagnostic, 6 (21.4%) negative, 5 (17.8%) atypical, and 7 (25%) were positive for mucinous cystic neoplasm (MCN) pancreatic cyst aspirates were analyzed with PancreaSeq® at Mayo Clinic, Jacksonville between September 2021 and February 2023.

Results: Three non-diagnostic, two negative, three atypical, and two positive for MCN cysts were positive for KRAS and GNAS mutations. They were interpreted as intraductal papillary mucinous neoplasm (IPMN) with low risk for progression to high-grade dysplasia/adenocarcinoma. One negative case was positive for KRAS and GNAS mutation and RNF43 copy number alteration. It was interpreted as IPMN with a low risk of progression. Two non-diagnostic, one negative, and two positive for MCN cysts were positive for KRAS mutation. All were interpreted as IPMN/MCNs with low risk of progression. One positive for MCN case was positive for GNAS mutation and ALK fusion and one positive for MCN case was positive for GNAS mutation, ALK fusion, and RNF43 copy number alteration. Both were interpreted as IPMN and their risk of progression was interpreted as not well understood. One atypical case was positive for KRAS and TP53 mutation and was interpreted as IPMN/ MCNs with a high risk of progression. VHL mutation was present in one non-diagnostic case. It was interpreted as serous cystadenoma and the risk for progression was low.

Conclusion: Molecular analysis of pancreatic cysts with PancreaSeq® is useful in accurate diagnosis, especially when cytologic material is non-diagnostic and helps improve patient management.

Objectives: This article aims at exploring the clinical value of high-risk human papillomavirus (HPV) positive and paired boxed 1 (PAXI)/zinc finger protein 582 (ZNF582) gene methylation shunt as a new approach for accurate cervical cancer screening.

Material and methods: Selecting 115 patients were treated in the Cervical Department of Xuzhou Matemal and Child Health Hospital from October 2018-October 2020. All patients underwent cervical exfoliated cell thinprep cytologic test (TCT) detection, HPV typing quantitative detection, and PAX1/ZNF582 gene methylation level Detection. Taking the biopsy pathological diagnosis under colposcopy as the gold standard, analyzing the test results statistically, and the sensitivity, specificity, and accuracy of the three screening methods alone and combined screening schemes were compared.

Results: Comparison of the three methods of cervical exfoliated cell TCT, HPV typing and quantification, and PAX1/ZNF582 methylation gene detection showed that the gene detection method has the highest specificity, 97.30%; The HPV typing quantitative detection has the highest sensitivity, 89.71%, but its specificity is poor; and the PAX1/ZNF582 gene detection has the highest accuracy.

Conclusion: For patients with high-grade cervical lesions and cervical cancer, PAX1/ZNF582 gene methylation level can be used as an important biomarker for the diagnosis and classification of cervical cancer. PAX1/ZNF582 methylation gene detection is effective in high-grade cervical lesions and cervical cancer. Screening has high clinical value and can become a new way of accurate cervical cancer screening.

Vulval fibroadenoma is an extremely rare lesion, mostly seen in young adults. A 51-years-old woman presented with a painless, mobile, and pedunculated vulval mass. Fine-needle aspiration (FNA) was performed and was diagnosed as a benign fibroepithelial lesion possibly fibroadenoma vulva, which was later confirmed as fibroadenoma vulva histopathologically. It is just not rare to find fibroadenoma vulva, but this should also be kept as a differential when diagnosing such cytomorphology in FNA lesions. This is important to avoid unnecessary incisional biopsy before excision.

Ameloblastoma is a benign progressively growing intraosseous epithelial odontogenic tumor. It is characterized by expansion and a tendency for a local recurrence if inadequately removed. Since it has an aggressive clinical course, surgical removal and histopathological examination should be done for appropriate management. In this case study, a 52-year-old female patient came to our institute with a complaint of swelling in the lower midline of gum. The patient has a history of bleeding gum and swelling 25 years back, for that she underwent tooth extraction in a private clinic. The patient again developed gum swelling year back, for that she underwent tooth extraction in a private clinic. However, this time her symptoms persisted, and for this reason, the patient visited our institute. On palpation, the lesion was firm and non-tender, appeared to arise from mandibular bone. Magnetic resonance imaging (multiplanar and multisequence) was done and it was reported as expansile multiseptate mandibular symphysis mass, possibly ameloblastoma. FNAC was done from the right lower alveolus in a private pathology laboratory, which was reported as pleomorphic adenoma with focal squamous metaplasia. These slides were reviewed in our institute and we reported it as suggestive of odontogenic tumor, favoring ameloblastoma. Biopsy and histopathological examination was advised for confirmation. Tumor was surgically enucleated along with curettage of the site and excised specimen was sent for histopathological examination to the pathology department of our institute. Based on the clinical, radiological, cytological, and histopathological examination, a final diagnosis of acanthomatous ameloblastoma was made. To the best of our knowledge, very few cases of acanthomatous type of ameloblastomas have been diagnosed on aspiration cytology followed by excision and histopathological confirmation. In this case study, we tried to highlight the importance of early diagnosis by cytology which helps in early treatment by surgical excision of this locally aggressive tumor.