Pub Date : 2020-03-25DOI: 10.23675/SJLAS.V46I1.989
By Jean-Marc Bertho, S. Ancelet, Delphine Denais-Laliève, Nicolas Dudoignon
A retrospective statistical study has been performed in order to identify the biological, clinical and behavioural variables that could potentially predict the survival status of irradiated non-human primates (NHP) and to refine the future use of humane end points (HEP). The available data come from experiments that were initially designed and implemented to evaluate the efficacy of various treatments of the radiation-induced hematopoietic syndrome. A group of 15 NHP was selected on the basis of similar irradiation and treatment protocols. Among those 15 NHP, 3 animals died during the study despite efforts developed to prevent high suffering and death. Among individual variables collected during the study, the ones retained for the present analysis were Flt3-ligand concentration in plasma, body temperature, alimentary behaviour and general behaviour score. A Bayesian predictive analysis showed that, although some of these variables were of potential interest, none of them alone proved valuable in predicting the death of the three animals. A refined analysis showed that a rapid decrease in body temperature during 3 consecutive days of more than 2.5°C was predictive of the death of two out of three animals. Moreover, a combination of several variables including decrease in body temperature and general behaviour score, improved the prediction of death. These results confirm that a HEP cannot only rely on the use of a single variable, strongly suggesting using a combination of variables, including behaviour, as a composite HEP in NHP experiments using high doses of irradiation.
{"title":"HUMANE ENDPOINTS IN SEVERE IRRADIATION EXPERIMENTS USING NON-HUMAN PRIMATES: A RETROSPECTIVE ANALYSIS.","authors":"By Jean-Marc Bertho, S. Ancelet, Delphine Denais-Laliève, Nicolas Dudoignon","doi":"10.23675/SJLAS.V46I1.989","DOIUrl":"https://doi.org/10.23675/SJLAS.V46I1.989","url":null,"abstract":"A retrospective statistical study has been performed in order to identify the biological, clinical and behavioural variables that could potentially predict the survival status of irradiated non-human primates (NHP) and to refine the future use of humane end points (HEP). The available data come from experiments that were initially designed and implemented to evaluate the efficacy of various treatments of the radiation-induced hematopoietic syndrome. A group of 15 NHP was selected on the basis of similar irradiation and treatment protocols. Among those 15 NHP, 3 animals died during the study despite efforts developed to prevent high suffering and death. Among individual variables collected during the study, the ones retained for the present analysis were Flt3-ligand concentration in plasma, body temperature, alimentary behaviour and general behaviour score. A Bayesian predictive analysis showed that, although some of these variables were of potential interest, none of them alone proved valuable in predicting the death of the three animals. A refined analysis showed that a rapid decrease in body temperature during 3 consecutive days of more than 2.5°C was predictive of the death of two out of three animals. Moreover, a combination of several variables including decrease in body temperature and general behaviour score, improved the prediction of death. These results confirm that a HEP cannot only rely on the use of a single variable, strongly suggesting using a combination of variables, including behaviour, as a composite HEP in NHP experiments using high doses of irradiation.","PeriodicalId":49571,"journal":{"name":"Scandinavian Journal of Laboratory Animal Science","volume":"51 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2020-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73213091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.23675/SJLAS.V45I0.581
Sini K. Koski, Sakari Leino, S. Rannanpää, O. Salminen
Various ways exist to establish rodent models of Parkinson’s disease, a neurodegenerative motor disorder caused by the death of dopaminergic neurons that have their cell bodies in the substantia nigra pars compacta (SNc) and project into the dorsal striatum. Neurodegeneration and motor symptoms of Parkinson’s disease can be mimicked in animals by degenerating these nigrostriatal neurons with different neurotoxins, administered either systemically or intracranially, or by genetic manipulations (Bové & Perier, 2012). Importantly, different methods can cause difImplementation of improved postoperative care decreases the mortality rate of operated mice after an abundant 6-hydroxydopamine lesion of nigrostriatal dopaminergic neurons
{"title":"Implementation of improved postoperative care decreases the mortalit1y rate of operated mice after an abundant 6-hydroxy dopamine lesion of nigrostriatal dopaminergic neurons","authors":"Sini K. Koski, Sakari Leino, S. Rannanpää, O. Salminen","doi":"10.23675/SJLAS.V45I0.581","DOIUrl":"https://doi.org/10.23675/SJLAS.V45I0.581","url":null,"abstract":"Various ways exist to establish rodent models of Parkinson’s disease, a neurodegenerative motor disorder caused by the death of dopaminergic neurons that have their cell bodies in the substantia nigra pars compacta (SNc) and project into the dorsal striatum. Neurodegeneration and motor symptoms of Parkinson’s disease can be mimicked in animals by degenerating these nigrostriatal neurons with different neurotoxins, administered either systemically or intracranially, or by genetic manipulations (Bové & Perier, 2012). Importantly, different methods can cause difImplementation of improved postoperative care decreases the mortality rate of operated mice after an abundant 6-hydroxydopamine lesion of nigrostriatal dopaminergic neurons","PeriodicalId":49571,"journal":{"name":"Scandinavian Journal of Laboratory Animal Science","volume":"241 1","pages":"1-11"},"PeriodicalIF":0.8,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85152549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.23675/SJLAS.V43I0.492
L. Astrup, T. Iburg, J. Agerholm, B. Aalbæk, O. L. Nielsen, P. S. Leifsson
{"title":"Local infusion of Staphylococcus aureus into the porcine internal carotid artery as a model of sepsis-related brain abscesses - A pilot study","authors":"L. Astrup, T. Iburg, J. Agerholm, B. Aalbæk, O. L. Nielsen, P. S. Leifsson","doi":"10.23675/SJLAS.V43I0.492","DOIUrl":"https://doi.org/10.23675/SJLAS.V43I0.492","url":null,"abstract":"","PeriodicalId":49571,"journal":{"name":"Scandinavian Journal of Laboratory Animal Science","volume":"1 1","pages":"7"},"PeriodicalIF":0.8,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82179878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J R Mader, M A Mason, L K Bale, N M Gades, C A Conover
The purpose of this study was to ascertain if prophylactic ingestion of a diet rich in vitamin E would prevent or impede the development of ulcerative dermatitis in mice on a C57BL/6 background. Mice were fed either a standard mouse diet, vitamin E (99 IU/kg), or a mouse diet fortified with vitamin E (3000 IU/kg) after weaning. Cases of ulcerative dermatitis were recorded by individuals unmasked to the diet assignment. The incidence of ulcerative dermatitis in a retrospective cohort of mice on standard diet was compared with the group on the diet fortified with vitamin E. Age was associated with ulcerative dermatitis in standard diet and vitamin E fortified diet groups, r = 0.43, p-value < 0.0001 and r = 0.18, p-value < 0.02, respectively. The average age of incidence for ulcerative dermatitis in the mice fed the standard diet was 89 weeks and for the mice fed the vitamin E diet it was 41 weeks. The unadjusted odds ratio comparing the incidence of ulcerative dermatitis between the two diet groups was 4.6 with a 95% confidence interval of (2.44, 8.58), χ(2) p-value < 0.0001. Therefore, there was an association between the diets and ulcerative dermatitis, with the mice on the vitamin E fortified diet having almost five times the odds of having ulcerative dermatitis compared with mice on the standard diet. Incidence of ulcerative dermatitis was not influenced by sex or genotype. Our study results show that a diet fortified in vitamin E initiated at weaning does not prevent or impede the development of ulcerative dermatitis in mice on a C57BL/6 background and may accelerate development when administered to young mice.
{"title":"The Association of Early Dietary Supplementation with Vitamin E with the Incidence of Ulcerative Dermatitis in Mice on a C57BL/6 Background: Diet and Ulcerative Dermatitis in Mice.","authors":"J R Mader, M A Mason, L K Bale, N M Gades, C A Conover","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The purpose of this study was to ascertain if prophylactic ingestion of a diet rich in vitamin E would prevent or impede the development of ulcerative dermatitis in mice on a C57BL/6 background. Mice were fed either a standard mouse diet, vitamin E (99 IU/kg), or a mouse diet fortified with vitamin E (3000 IU/kg) after weaning. Cases of ulcerative dermatitis were recorded by individuals unmasked to the diet assignment. The incidence of ulcerative dermatitis in a retrospective cohort of mice on standard diet was compared with the group on the diet fortified with vitamin E. Age was associated with ulcerative dermatitis in standard diet and vitamin E fortified diet groups, r = 0.43, p-value < 0.0001 and r = 0.18, p-value < 0.02, respectively. The average age of incidence for ulcerative dermatitis in the mice fed the standard diet was 89 weeks and for the mice fed the vitamin E diet it was 41 weeks. The unadjusted odds ratio comparing the incidence of ulcerative dermatitis between the two diet groups was 4.6 with a 95% confidence interval of (2.44, 8.58), χ(2) p-value < 0.0001. Therefore, there was an association between the diets and ulcerative dermatitis, with the mice on the vitamin E fortified diet having almost five times the odds of having ulcerative dermatitis compared with mice on the standard diet. Incidence of ulcerative dermatitis was not influenced by sex or genotype. Our study results show that a diet fortified in vitamin E initiated at weaning does not prevent or impede the development of ulcerative dermatitis in mice on a C57BL/6 background and may accelerate development when administered to young mice.</p>","PeriodicalId":49571,"journal":{"name":"Scandinavian Journal of Laboratory Animal Science","volume":"37 4","pages":"253-259"},"PeriodicalIF":0.8,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3503310/pdf/nihms298972.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31072648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Nireekshan Kumar, P. Nagarajan, R. Venkatesan, R. Juyal
Spontaneous cases of rectal prolapse in a breeding colony of FVB mice were found to be due to infection with Syphacia obvelata and Citrobacter freundii. Microbiology, biochemical and parasitological examination revealed Citrobacter freundii and eggs of Syphacia obvelata. After treatment with antibiotics, antihelminthic drugs and manual reduction prevented further occurrence.
{"title":"Case Report and Short Communication: Rectal prolapse associated with an unusual combination of pinworms and citrobacter species infection in FVB mice colony","authors":"M. Nireekshan Kumar, P. Nagarajan, R. Venkatesan, R. Juyal","doi":"10.23675/SJLAS.V31I4.72","DOIUrl":"https://doi.org/10.23675/SJLAS.V31I4.72","url":null,"abstract":"Spontaneous cases of rectal prolapse in a breeding colony of FVB mice were found to be due to infection with Syphacia obvelata and Citrobacter freundii. Microbiology, biochemical and parasitological examination revealed Citrobacter freundii and eggs of Syphacia obvelata. After treatment with antibiotics, antihelminthic drugs and manual reduction prevented further occurrence.","PeriodicalId":49571,"journal":{"name":"Scandinavian Journal of Laboratory Animal Science","volume":"37 1","pages":"221-223"},"PeriodicalIF":0.8,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82757542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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