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Knowledge and practice on the use of alcohol and coffee among final-year undergraduate students of Hanoi Medical University in 2023 河内医科大学2023年本科毕业班学生对酒精和咖啡使用的知识和实践
Pub Date : 2023-09-27 DOI: 10.47866/2615-9252/vjfc.4123
Quy Vu Thi
Regular use of alcohol and coffee may be harmful to human health. Previous studies showed that students who consumed alcohol may consume coffee as well. We conducted a cross-sectional study with 212 final–year undergraduate students of Hanoi Medical University to describe their knowledge and practice in alcohol and coffee use The results show that over 70% of students had knowledge of alcohol and coffee. Approximately 87% and 41% of students thought that alcohol and coffee are both beneficial and harmful to health, respectively. Regarding the actual practice in the use of alcohol and coffee, 93.4% of students had ever drunk alcohol; while 84.9% of those had ever drunk coffee. The frequency of alcohol and coffee use is mostly less than 4 days per month. We found a high percentage of students had knowledge of alcohol and coffee, and they had been using alcohol and/or coffee, however, they occasionally used those drinks with a monthly frequency. It is necessary to strengthen communication activities about alcohol use and its health effects, especially recommending that alcohol use should be limited to less than 2 units/day in men, below 1 unit/day in women, and no more than 5 days/week [1].
经常饮用酒精和咖啡可能对人体健康有害。先前的研究表明,喝酒的学生可能也会喝咖啡。我们对河内医科大学的212名大四本科生进行了横断面研究,以描述他们在酒精和咖啡使用方面的知识和实践。结果显示,超过70%的学生有酒精和咖啡的知识。大约87%的学生和41%的学生分别认为酒精和咖啡对健康有益和有害。在实际使用酒精和咖啡方面,93.4%的学生曾经喝过酒;而84.9%的人曾经喝过咖啡。饮酒和喝咖啡的频率一般每个月少于4天。我们发现有很高比例的学生知道酒精和咖啡,并且他们一直在使用酒精和/或咖啡,然而,他们偶尔以每月的频率使用这些饮料。有必要加强关于饮酒及其对健康影响的宣传活动,尤其建议男性饮酒量应控制在2单位/天以下,女性饮酒量应控制在1单位/天以下,每周饮酒量不超过5天[1]。
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引用次数: 0
Initial research on the application of the liquid chromatography - quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) method for monitoring pesticide residues in fruits and vegetables 液相色谱-四极杆飞行时间质谱法(LC-QTOF-MS)在果蔬中农药残留监测中的应用初探
Pub Date : 2023-09-22 DOI: 10.47866/2615-9252/vjfc.4122
Huy Dinh Dang
Currently, there are thousands of plant protection chemicals being allowed for use in agriculture. Abuse or improper use of pesticides lead to increase residues in products, exceeding the maximum residue limit according to the regulations. Therefore, the need for developing methods, which can monitor, detect, and identify many pesticides simultaneously is necessary. The liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-QTOF-MS) method has advantage of being able to simultaneously quantify and screen many compounds at once, are being a trend in analytical techniques for analyzing and screening pesticide residues in foods. In this study, the LC-QTOF-MS method was used to quantify 186 pesticides in fruit and vegetable samples. The limit of detection (LOD) for all compounds is 3.0 µg/kg. The recovery is in the ranged of 74.4 -118%, relative standard deviation (RSD) <20%. At the same time, the method was initial researched for nontarget screening by reprocessing previously acquired data without re-injection of the samples. The list of pesticides can be screening, which are up to 500 chemicals, by comparison results with the available high resolution mass spectral library. The screening detection limit of the method (SDL) is 100 µg/kg. The method has been applied to quantify 186 pesticides, and nontarget screening analyse in 160 fruit and vegetable samples in the first 06 months of 2023.
目前,有成千上万种植物保护化学品被允许用于农业。滥用或不当使用农药导致产品残留增加,超过规定的最大残留限量。因此,有必要开发一种能够同时监测、检测和识别多种农药的方法。液相色谱四极杆飞行时间质谱法(LC-QTOF-MS)具有同时对多种化合物进行定量和筛选的优点,是食品中农药残留分析筛选技术的发展趋势。本研究采用LC-QTOF-MS方法对果蔬样品中186种农药进行了定量分析。所有化合物的检出限(LOD)均为3.0微g/kg。回收率在74.4 ~ 118%之间,相对标准偏差(RSD)为20%。同时,对该方法进行了初步研究,通过对先前获取的数据进行再处理,无需回注样品进行非靶标筛选。通过与现有的高分辨率质谱库比对,可筛选农药清单,最多可筛选500种化学物质。该方法的筛选检出限(SDL)为100 μ g/kg。该方法已于2023年前06个月对186种农药进行了定量分析,并对160种果蔬样品进行了非靶向筛选分析。
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引用次数: 0
Simultaneous determination of crocin and crocetin in food samples by highperformance liquid chromatography (HPLC-PDA) 高效液相色谱(HPLC-PDA)同时测定食品中藏红花素和藏红花素
Pub Date : 2023-09-13 DOI: 10.47866/2615-9252/vjfc.4121
Thuy Do Thi Hong
Crocin and crocetin are two bioactive compounds found in saffron and gardenia. In the food industry, due to their natural yellow or orange color, they are used as food colouring. The study was carried out to optimize the high-performance liquid chromatography combined with a photodiode detector (HPLC-PDA) to simultaneously determine the crocin and crocetin content in food. Analytical samples were ultrasonicated with ACN:H2O (1:1, v/v) solvent for 30 minutes. The sample solution was determined by HPLC – PDA with the following conditions: Sunfire C18 column (250 mm x 4.6 mm; 5 µm) mobile phase consisting of 0.1% formic acid and ACN using gradient program. The method has good specificity, the standard curves of crocin and crocetin have correlation coefficients R2 > 0.999, respectively, repeatability and recovery meet AOAC requirements. The limit of detection (LOD) for  crocin and crocetin were 0.5 mg/kg and 0.25 mg/kg, respectively. The method has been applied to simultaneously determine crocin and crocetin in 15 real samples and results in 13 out of 15 samples detecting crocin content, 11 out of 15 samples detecting crocetin content, and in 2 out out of 15 samples. neither crocin nor crocetin was detected. The gardenia powder group contained the highest content of crocin and crocetin (23.5 g/100g – 24.4 g/100g) and dried lychee samples - sun-dried or dried gardenia contains the second highest concentrations of crocin and crocetin in the food group.
藏红花素和藏红花素是在藏红花和栀子花中发现的两种生物活性化合物。在食品工业中,由于其天然的黄色或橙色,被用作食品着色剂。本研究优化了高效液相色谱法结合光电二极管检测器(HPLC-PDA)同时测定食品中藏红花素和藏红花素含量的方法。分析样品用ACN:H2O (1:1, v/v)溶剂超声处理30分钟。采用高效液相色谱法测定样品溶液;PDA与以下条件:Sunfire C18柱(250 mm x 4.6 mm;5微米)流动相组成的0.1%甲酸和ACN使用梯度程序。方法特异性好,藏红花素与藏红花素标准曲线的相关系数为R2 >重复性和回收率分别为0.999,符合AOAC要求。检出限(LOD)藏红花素和藏红花素分别为0.5 mg/kg和0.25 mg/kg。应用该方法同时测定了15个实际样品中的藏红花素和藏红花素,15个样品中有13个样品检测到藏红花素含量,15个样品中有11个样品检测到藏红花素含量,15个样品中有2个样品检测到藏红花素含量。藏红花素和藏红花素均未检出。栀子粉组藏红花素和藏红花素含量最高(23.5 g/100g;24.4 g/100g)和晒干的荔枝样品-晒干或晒干的栀子花在食物组中含有第二高的藏红花素和藏红花素浓度。
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引用次数: 0
Microbiological contamination of the inner surface of finished water filling taps at bottled drinking water producers in Khanh Hoa province 庆化省瓶装饮用水生产商成品充水龙头内表面的微生物污染
Pub Date : 2023-08-28 DOI: 10.47866/2615-9252/vjfc.4119
Phong Le Quoc
The finished water filling tap is the final contact point of filtered drinking water before bottling. Therefore, microbial contamination at this site may result in cross-contamination into the finished water in the bottle. This study was conducted to evaluate microbiological contamination on the inner surface of the finished water filling tap at the bottled drinking water producers in Khanh Hoa in 2022. A total of 93 swab samples of the inside surface of the tap at 93 producers were subjected to a test for ATP (Adenosine Triphosphate), total plate count (TPC), coliform, Escherichia coli, Pseudomonas aeruginosa, Streptococci feacal, and Clostridia. There were 72/93 (77.4%) samples with ATP values higher than 300 RLU/cm2. The geometric mean of ATP and TPC were 3.1±0.8 log (RLU/cm2) and 3.5±1.2 log (CFU/cm2), respectively. The ATP and TPC were linear correlated, indicating that the surface ATP value inside the finished water filling tap was mainly formed by microorganisms. In addition, 6/93 (6.5%) samples were found to be positive for coliform, including 3 strains of Enterobacter cloacae, 2 strains of Klebsiella pneumoniae and 1 strain of Enterobacter aerogenes. Moreover, 1/93 (1.1%) samples were infected with P. aeruginosa. All 7 strains of these bacteria were biofilm producers under laboratory conditions. Our results demonstrated that the cleaning and decontamination of finished water filling taps were not properly processed, thereby increasing the risk of microbial contamination into finished bottled water.
成品充水龙头是装瓶前过滤饮用水的最后接触点。因此,在这个地方的微生物污染可能导致交叉污染进入瓶中的成品水。本研究旨在评估2022年庆化省瓶装饮用水生产商成品充水龙头内表面的微生物污染情况。对93家生产商的水龙头内表面的93个棉签样本进行了ATP(三磷酸腺苷)、总平板计数(TPC)、大肠菌群、大肠杆菌、铜绿假单胞菌、粪便链球菌和梭状芽胞杆菌的检测。有72/93(77.4%)样品的ATP值高于300 RLU/cm2。ATP和TPC的几何平均值分别为3.1±0.8 log (RLU/cm2)和3.5±1.2 log (CFU/cm2)。ATP与TPC呈线性相关,说明成品充水龙头内部的表面ATP值主要由微生物形成。大肠菌群检出6/93株(6.5%),其中阴沟肠杆菌3株、肺炎克雷伯菌2株、产气肠杆菌1株。此外,1/93(1.1%)的样品感染了铜绿假单胞菌。在实验室条件下,这7株细菌都能产生生物膜。我们的研究结果表明,成品瓶装水的清洁和净化处理不当,从而增加了成品瓶装水被微生物污染的风险。
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引用次数: 0
A mini-review on analytical methods for polycyclic aromatic hydrocarbons in Vietnamese food and beverage samples 越南食品饮料样品中多环芳烃分析方法综述
Pub Date : 2023-08-24 DOI: 10.47866/2615-9252/vjfc.4114
Anh Hoang Quoc
Polycyclic aromatic hydrocarbons (PAHs) are a common class of organic pollutants in the environment and are of great research interest because of their high toxicity, including carcinogenicity and mutagenicity. Food is an important source of PAHs exposure in humans, so analysis of PAHs in food is an essential task of food safety testing. This review article gathers information about the analysis methods of PAHs that has been applied in previous studies on food and beverage samples in Vietnam, including steps of extraction, purification of extracts, and quantitative analysis on a chromatographic system. PAHs are extracted from the sample matrix into less polar organic solvents with different techniques such as liquidliquid, liquid-solid, ultrasonic extraction, and pressurized liquid extraction. Interferences in sample extracts can be removed by various techniques: acid and alkali treatment, gel permeation chromatography, solid phase extraction, and dispersive solid phase extraction. PAHs are separated and quantified on gas chromatography-mass spectrometry systems (GCMS, GC-MS/MS, GC×GC-ToFMS) or liquid chromatograph with fluorescence detector (HPLC-FLD). Analytical procedures are all quality controlled by results with blank samples, limits of detection/quantification, recovery standards, and replicate samples. Overall studies to evaluate the content of PAHs in a variety of food samples need to continue to be performed to establish safe consumption thresholds for this group of pollutants in Vietnam.
多环芳烃(PAHs)是环境中常见的一类有机污染物,因其具有致癌性和致突变性等高毒性而备受关注。食品是人体接触多环芳烃的重要来源,因此食品中多环芳烃的分析是食品安全检测的重要内容。本文综述了越南食品和饮料样品中多环芳烃的分析方法,包括提取、纯化和色谱定量分析的步骤。采用液液萃取、液固萃取、超声萃取、加压萃取等不同的萃取工艺,将多环芳烃从样品基质中提取到极性较小的有机溶剂中。样品提取物中的干扰可通过酸碱处理、凝胶渗透色谱、固相萃取和分散固相萃取等技术去除。多环芳烃可通过气相色谱-质谱联用系统(GCMS、GC-MS/MS、gc - times、GC-ToFMS)或带荧光检测器(HPLC-FLD)的液相色谱仪分离和定量。分析过程的质量由空白样品的结果、检测/定量限、回收率标准和重复样品控制。需要继续进行全面的研究来评估各种食品样品中多环芳烃的含量,以确定越南这类污染物的安全消费阈值。
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引用次数: 0
Determination performance of ICP-MS heavy metals in food and feed test method through participation of proficiency testing programs 参与能力测试计划的ICP-MS测定食品和饲料中重金属的测试方法
Pub Date : 2023-08-24 DOI: 10.47866/2615-9252/vjfc.4117
Giang Tran Hoang
The inductively coupled plasma mass spectrometry (ICP-MS) method is applied to determine the content of heavy metals (Pb, Cd, As, Hg) in food and animal feed samples at the National Institute for Food Control due to its high accuracy and quick response time. These samples were digested in a microwave using a mixture of nitric acid and hydrogen peroxide, with the yttrium internal standard added initially to control the analysis. The efficiency of the method was evaluated to meet the requirements of AOAC with reasonable specificity, working standard curve for elements in the range from 0.4 to 20 µg/L, the relative standard deviation of repeatability RSDr < 3,55%, and the recoveries (R%) ranged from 92.2 to 103.1%. The method has been validated by participating in international proficiency testing programs in 2021 and 2022 with a value of |z-score| ≤ 2. Therefore, the results affirm the laboratory's competency meets the requirements for the validity of test results ISO/IEC 17025 requirements, reliability for customers and authorities.
电感耦合等离子体质谱法(ICP-MS)测定食品和动物饲料样品中重金属(Pb、Cd、As、Hg)的准确度高,反应时间快,应用于国家食品控制研究所。这些样品采用硝酸和过氧化氢的混合物在微波中消解,初始加入钇内标以控制分析。评价了该方法的效率,以合理的特异性满足AOAC的要求,元素在0.4 ~ 20 μ g/L范围内的工作标准曲线,重复性RSDr的相对标准偏差;回收率(R%)在92.2% ~ 103.1%之间。该方法已通过参加2021年和2022年的国际熟练度测试项目得到验证,其值为z-score ≤2. 因此,结果确认实验室的能力符合ISO/ iec17025对测试结果有效性的要求,对客户和主管部门的可靠性要求。
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引用次数: 0
Students' knowledge, attitude, and practice on food safety in Mekong University from July 2022 to December 2022 2022年7月至2022年12月湄公河大学学生对食品安全的知识、态度和实践
Pub Date : 2023-07-28 DOI: 10.47866/2615-9252/vjfc.4120
Thy Vuong Bao
Food safety affects to physical and mental development of human. This article observed the knowledge, attitude and practice of students on food safety to survey students' knowledge, attitude and practice on food safety. A cross sectional description study was performed on 309 students in Cuu Long university in the Mekong Delta region. SPSS 16.0 was used to analyze the data. The results showed that in term of knowledge of food safety: 68.28% of students had knowledge of food poisoning, 75.73% could detect the risk of food poisoning, 80.58% described the clinical symptoms of food poisoning, and 62.46% knew the World Health Organization's golden rule of food safety. Regarding the knowledge of food preservation: 69.26%-75,73% had knowledge of egg preservation and of processed foods. For knowledge of solving food poisoning: 80.26% had knowledge of solving food poisoning cases. Attitude of food safety: 83.82% knew choosing the place to buy food safely. Practice of food safety: 68.93%-81.55% had good practiced in choosing safe fresh food and processed food.
食品安全关系到人的身心发展。本文通过对学生食品安全知识、态度和行为的观察,调查学生对食品安全的知识、态度和行为。本文对湄公河三角洲库龙大学309名学生进行了横断面描述研究。采用SPSS 16.0 软件对数据进行分析。结果显示,在食品安全知识方面:68.28%的学生有食物中毒知识,75.73%的学生能发现食物中毒的风险,80.58%的学生能描述食物中毒的临床症状,62.46%的学生知道世界卫生组织的食品安全黄金法则。关于食品保鲜知识:69.26%- 75%,73%的人有鸡蛋保鲜知识和加工食品知识。在解决食物中毒案件知识方面,有解决食物中毒案件知识的占80.26%。对食品安全的态度:83.82%的人知道如何选择安全的地方购买食品。食品安全实践:68.93%-81.55%的人在选择安全的新鲜食品和加工食品方面有良好的实践。
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引用次数: 0
Studying microscopic characteristics and examination of the factors effect flavonoid contents extracted from Bishop wood (Bischofia javanica Blume) leaves at Lam Dong province 研究了林东毕晓木叶中黄酮类化合物的显微特征及影响因素
Pub Date : 2023-06-28 DOI: 10.47866/2615-9252/vjfc.4116
Tam Tran Mong To
In recent years, the use of natural herbal remedies in disease treatment in Vietnam has been significantly considered. Research on the chemical composition, microscopic characteristics, and extraction of active components from herbal materials play an important role in medicinal applications. This study aims to describe the microscopic features of fresh Bishopwood leaves using a dual staining method, examine leaf powder under a microscope, determine the components using chemical reactions, and investigate the influence of factors such as ethanol concentration, time, temperature, and the ratio of raw materials to solvents on the process of extracting flavonoids from Bishopwood leaves. Through unique chemical reactions, the components of Bishopwood leaves were identified to include flavonoids, phenolics, tannins, steroids, proteins, carbohydrates, and anthranoids. Microscopic examination of fresh Bishopwood leaves and leaf powder under a microscope revealed characteristic structural components including epidermis, stomata, parenchyma, sclerenchyma, fiber bundles, and vascular bundles. The appropriate conditions for extracting flavonoids from Bishopwood leaves were using 50% ethanol as the extraction solvent, a 3-hour extraction time, a temperature of 60 °C, and a raw material-to-solvent ratio of 1 : 30 (g/mL). The research findings serve as valuable information contributing to the identification, standardization, and development of new drugs from local medicinal materials.
近年来,在越南的疾病治疗中使用天然草药得到了广泛的考虑。中草药的化学成分、显微特征及有效成分的提取研究在药用中具有重要作用。本研究旨在采用双染色法描述新鲜毕晓木叶的微观特征,在显微镜下观察叶粉,利用化学反应测定成分,考察乙醇浓度、时间、温度、原料溶剂比等因素对毕晓木叶中黄酮类化合物提取过程的影响。通过独特的化学反应,鉴定出毕肖普叶的成分包括类黄酮、酚类物质、单宁、类固醇、蛋白质、碳水化合物和类蒽酮。在显微镜下观察新鲜的毕晓普叶和叶粉,发现其特有的结构成分包括表皮、气孔、薄壁组织、厚壁组织、纤维束和维管束。以50%乙醇为提取溶剂,提取时间为3 h,温度为60℃,料液比为1:30 (g/mL),提取双叶黄酮的适宜条件。研究结果为本地药材的鉴定、标准化和新药开发提供了有价值的信息。
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引用次数: 0
Simultaneous determination of benzyldimethyloctylammonium chloride and benzyldimethyldodecylammonium chloride by CE-C4D CE-C4D法同时测定苄基二甲基辛基氯化铵和苄基二甲基十二烷基氯化铵
Pub Date : 2023-05-28 DOI: 10.47866/2615-9252/vjfc.4115
Chien Dinh Viet, Anh Huong Nguyen Thi
Benzyldimethyloctylammonium chloride (BAC-C8) and Benzyldimethyldodecyl ammonium chloride (BAC-C12) are bactericidal surfactants which widely used in the pharmaceutical industry as anti-microbial disinfectants in skin antiseptic solution. According to current international regulations, the standard of finished antiseptic solution must have regulations on the limit of preservatives when used in the formulation. In this study, simultaneous determination of BAC-C8 and BAC-C12 was performed by capillary electrophoresis using capacitively coupled contactless conductivity detection (CE-C4D) with analytical conditions including: fused silica capillary (50 µm ID.) with effective length of 40 cm, background electrolyte solution of 100 mM acetic acid (Ace) added 30% acetonitril (ACN); hydrodynamic sample injection at height of 10 cm in 20 s; separation voltage of +15 kV. The detection limit of BAC-C8 and BAC-C12 were 1.5 mg/L and 3.5 mg/L, respectively. This method has been applied to determine the content of BAC-C8 and BACC12 in the disinfectant solution samples.
苄基二甲基辛基氯化铵(BAC-C8)和苄基二甲基十二烷基氯化铵(BAC-C12)是两种具有杀菌作用的表面活性剂,在医药工业中广泛用作皮肤防腐液中的抗菌消毒剂。根据现行国际规定,成品防腐液的标准在配方中使用时必须有防腐剂限量的规定。本研究采用电容偶联非接触式电导率检测(CE-C4D)毛细管电泳法同时测定BAC-C8和BAC-C12,分析条件为:有效长度为40 cm的熔融石英毛细管(50 μ m),背景电解质溶液为100 mM乙酸(Ace),加入30%乙腈(ACN);水动力样品在20 s内在10 cm高度注射;分离电压+ 15amp;nbsp;kV。BAC-C8和BAC-C12的检出限分别为1.5 mg/L和3.5 mg/L。应用该方法测定了消毒剂溶液样品中BAC-C8和BACC12的含量。
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引用次数: 0
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