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Caregivers' difficulty in managing sleep behaviors of children with attention-deficit/hyperactivity disorder and related factors during the COVID-19 pandemic. COVID-19大流行期间照顾者在管理注意力缺陷/多动障碍儿童睡眠行为方面的困难及其相关因素
IF 3.3 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2023-05-01 DOI: 10.1002/kjm2.12667
Ching-Shu Tsai, Ray C Hsiao, Cheng-Fang Yen
Dear Editor Changes in daily routines, closure of schools, reducing outdoor activities, and psychological stress due to the COVID-19 pandemic may change sleep behaviors in children during the COVID-19 pandemic. Managing sleep behaviors of children is a challenge encountered by the caregivers of children with attention-deficit/ hyperactivity disorder (ADHD). Given that sleep disturbances may worsen child psychosocial quality of life and impaired child daily functioning, caregivers' difficulty in managing sleep behaviors of children with ADHD during the COVID-19 pandemic and the factors related to this difficulty warrant further investigation. We recruited 252 caregivers (200 women and 52 men; mean age = 42.23 years; SD = 8.34 years) of children with ADHD from the child psychiatric outpatient clinics of two medical centers in Taiwan into this study between August 2021 and January 2022. The mean age of their children (200 boys and 52 girls) was 9.61 (SD = 2.39)
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引用次数: 0
Mechanism of human chorionic gonadotropin in endometrial receptivity via the miR-126-3p/PI3K/Akt/eNOS axis. 人绒毛膜促性腺激素通过miR-126-3p/PI3K/Akt/eNOS轴影响子宫内膜容受性的机制
IF 3.3 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2023-05-01 DOI: 10.1002/kjm2.12672
Wei Wang, Liang Ge, Li-Li Zhang, Li-Rong Wang, Yong-Yan Lu, Li Gou, Rui-Qiang Gou, Tong-Yu Xu, Xiao-Ling Ma, Xue-Hong Zhang

Human chorionic gonadotropin (hCG) might affect endometrial receptivity, exerting integral roles in embryo implantation. This study explored the action of hCG in endometrial receptivity via the miR-126-3p/PIK3R2/PI3K/Akt/eNOS axis. The embryo implantation dysfunction (EID) mouse models were established by administrating mifepristone and human endometrial epithelial cells (EECs) were used for in vivo experiments, both followed by hCG treatment. Expression level of CD105 and protein levels of cadherin CD144 and CD146 in mice were determined by immunohistochemistry and Western blot. The levels of miR-126-3p and PIK3R2 mRNA and PIK3R2, p-PI3K p85 α, PI3K p110 α, p-Akt, Akt, p-eNOS, and eNOS protein levels were measured. Cell proliferation was evaluated by CCK-8 and EdU assays. The binding sites of miR-126-3p and PIK3R2 were predicted and verified. hCG-treated EECs were further transfected with miR-126-inhibitor for functional rescue experiments. hCG ameliorated endometrial receptivity in EID mice. Moreover, hCG promoted miR-126-3p and suppressed PIK3R2 in EID mice and EECs. miR-126-3p targeted PIK3R2. EEC proliferation was enhanced after hCG treatment but inhibited by miR-126-3p downregulation. Both in vivo and in vitro experiments validated that hCG activated the PI3K/Akt/eNOS pathway through the miR-126-3p/PIK3R2 axis. Collectively, hCG improves endometrial receptivity by activating the PI3K/Akt/eNOS pathway via regulating miR-126-3p/PIK3R2.

人绒毛膜促性腺激素(hCG)可能影响子宫内膜容受性,在胚胎着床中发挥着不可或缺的作用。本研究通过miR-126-3p/PIK3R2/PI3K/Akt/eNOS轴探讨hCG在子宫内膜容受性中的作用。用米非司酮建立胚胎植入功能障碍(EID)小鼠模型,用人子宫内膜上皮细胞(EECs)进行体内实验,然后用hCG处理。免疫组化和Western blot检测小鼠CD105的表达水平和钙粘蛋白CD144、CD146的表达水平。检测miR-126-3p和PIK3R2 mRNA水平以及PIK3R2、p-PI3K p85 α、PI3K p110 α、p-Akt、Akt、p-eNOS和eNOS蛋白水平。CCK-8和EdU检测细胞增殖情况。预测并验证miR-126-3p和PIK3R2的结合位点。进一步用miR-126-inhibitor转染hcg处理的EECs进行功能挽救实验。hCG改善EID小鼠子宫内膜容受性。此外,hCG在EID小鼠和EECs中促进miR-126-3p,抑制PIK3R2。miR-126-3p靶向PIK3R2。hCG处理后EEC增殖增强,miR-126-3p下调抑制。体内和体外实验均证实hCG通过miR-126-3p/PIK3R2轴激活PI3K/Akt/eNOS通路。总的来说,hCG通过调节miR-126-3p/PIK3R2激活PI3K/Akt/eNOS通路,从而改善子宫内膜容受性。
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引用次数: 1
Formosanin C suppresses cancer cell proliferation and migration by impeding autophagy machinery. 台塑素C通过阻碍癌细胞自噬机制来抑制癌细胞的增殖和迁移。
IF 3.3 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2023-05-01 DOI: 10.1002/kjm2.12658
Man-Ling Chu, Pei-Wen Lin, Yu-Wen Liu, Shan-Ying Wu, Sheng-Hui Lan, Chun-Li Su, Hsiao-Sheng Liu

Formosanin C (FC) is a natural compound extracted from Paris formosana Hayata with anticancer activity. FC induces both autophagy and apoptosis in human lung cancer cells. FC-induced depolarization of mitochondrial membrane potential (MMP) may trigger mitophagy. In this study, we clarified the effect of FC on autophagy, mitophagy, and the role of autophagy in FC-related cell death and motility. We found FC caused the continuous increase of LC3 II (representing autophagosomes) from 24 to 72 h without degradation after treatment of lung and colon cancer cells, indicating that FC blocks autophagic progression. In addition, we confirmed that FC also induces early stage autophagic activity. Altogether, FC is not only an inducer but also a blocker of autophagy progression. Moreover, FC increased MMP accompanied by overexpression of COX IV (mitochondria marker) and phosphorylated Parkin (p-Parkin, mitophagy marker) in lung cancer cells, but no colocalization of LC3 with COX IV or p-Parkin was detected under confocal microscopy. Moreover, FC could not block CCCP (mitophagy inducer)-induced mitophagy. These results imply that FC disrupts mitochondria dynamics in the treated cells, and the underlying mechanism deserves further exploration. Functional analysis reveals that FC suppresses cell proliferation and motility through apoptosis and EMT-related pathway, respectively. In conclusion, FC acts as an inducer as well as a blocker of autophagy that results in cancer cell apoptosis and decreased motility. Our findings shed the light on the development of combined therapy with FC and clinical anticancer drugs for cancer treatment.

Formosanin C (FC)是一种天然化合物,从巴黎formosana Hayata中提取,具有抗癌活性。FC诱导人肺癌细胞自噬和凋亡。fc诱导的线粒体膜电位去极化(MMP)可能引发线粒体自噬。在本研究中,我们阐明了FC对自噬、有丝自噬的影响,以及自噬在FC相关细胞死亡和运动中的作用。我们发现,在肺癌和结肠癌细胞处理后,FC使LC3 II(代表自噬体)在24至72小时内持续增加而不降解,表明FC阻断了自噬进程。此外,我们证实了FC也能诱导早期自噬活动。总之,FC不仅是自噬进程的诱导剂,也是一种阻断剂。此外,在肺癌细胞中,FC增加了MMP,并伴有COX IV(线粒体标志物)和磷酸化Parkin (p-Parkin,线粒体自噬标志物)的过表达,但共聚焦显微镜下未检测到LC3与COX IV或p-Parkin的共定位。此外,FC不能阻断CCCP诱导的线粒体自噬。这些结果表明,FC破坏了处理细胞中的线粒体动力学,其潜在机制值得进一步探索。功能分析表明,FC分别通过凋亡和emt相关途径抑制细胞增殖和运动。综上所述,FC作为自噬的诱导剂和阻滞剂,导致癌细胞凋亡和运动能力下降。我们的研究结果揭示了与FC联合治疗和临床抗癌药物治疗癌症的发展。
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引用次数: 2
Distribution and clinical features of lymphomas involving skin in Taiwan. 台湾皮肤淋巴瘤之分布与临床特征。
IF 3.3 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2023-05-01 DOI: 10.1002/kjm2.12659
Cheng-Lin Wu, Su-Wei Chang, Julia Yu-Yun Lee, Tsai-Yun Chen, Chien-Chin Chen, Chia-Bao Chu, L Jeffrey Medeiros, Kung-Chao Chang

A wide variety of primary and secondary lymphoma types involves the skin. However, reports with comparisons between both groups are limited in Taiwan. We retrospectively enrolled all cutaneous lymphomas and evaluated their clinicopathologic features. There were 221 cases of lymphoma: 182 (82.3%) primary and 39 (17.7%) secondary. Mycosis fungoides was the most common primary T-cell lymphoma, 92 (41.7%) cases, followed by CD30-positive T-cell lymphoproliferative disorders including lymphomatoid papulosis (n = 33, 14.9%) and cutaneous anaplastic large cell lymphoma (n = 12, 5.4%). The most frequent primary B-cell lymphomas were marginal zone lymphoma (n = 8, 3.6%) and diffuse large B-cell lymphoma (DLBCL), leg type (n = 8, 3.6%). DLBCL including variants was the most common secondary lymphoma involving skin. Most primary lymphomas presented at low-stage (T-cell, 86%; B-cell, 75%), whereas the majority of secondary lymphomas presented at high-stage (T-cell, 94%; B-cell, 100%). Patients with secondary lymphomas had an older mean age, more frequent B symptoms, lower serum albumin and hemoglobin, and a higher frequency of atypical lymphocytes in blood than those with primary lymphomas. In primary lymphomas, older age, lymphoma types, decreased lymphocyte counts and atypical lymphocytes in blood were poorer prognostic factors. In secondary lymphoma patients, lymphoma types, high serum lactate dehydrogenase and low hemoglobin levels predicted poorer survival. We found that the distribution of primary cutaneous lymphomas in Taiwan mirrors that of other Asian countries but shows some differences as compared with Western countries. Primary cutaneous lymphomas have a better prognosis than secondary lymphomas. Histologic classification of lymphomas highly correlated with disease presentation and prognosis.

各种各样的原发性和继发性淋巴瘤类型涉及皮肤。然而,在台湾,对两组进行比较的报道有限。我们回顾性地纳入所有皮肤淋巴瘤并评估其临床病理特征。221例淋巴瘤:原发182例(82.3%),继发39例(17.7%)。真菌样真菌病是最常见的原发性t细胞淋巴瘤,92例(41.7%),其次是cd30阳性t细胞增生性疾病,包括淋巴瘤样丘疹病(n = 33, 14.9%)和皮肤间变性大细胞淋巴瘤(n = 12, 5.4%)。最常见的原发性b细胞淋巴瘤是边缘带淋巴瘤(n = 8, 3.6%)和弥漫性大b细胞淋巴瘤(DLBCL),腿型(n = 8, 3.6%)。包括变异体在内的DLBCL是最常见的累及皮肤的继发性淋巴瘤。大多数原发性淋巴瘤表现为低分期(t细胞,86%;b细胞,75%),而大多数继发性淋巴瘤出现在高分期(t细胞,94%;b细胞,100%)。继发性淋巴瘤患者比原发性淋巴瘤患者平均年龄更大,B症状更频繁,血清白蛋白和血红蛋白较低,血液中非典型淋巴细胞的频率更高。在原发性淋巴瘤中,年龄、淋巴瘤类型、淋巴细胞计数减少和血液中非典型淋巴细胞是较差的预后因素。在继发性淋巴瘤患者中,淋巴瘤类型、高血清乳酸脱氢酶和低血红蛋白水平预示较差的生存。我们发现台湾原发性皮肤淋巴瘤的分布与其他亚洲国家相同,但与西方国家相比有一些差异。原发性皮肤淋巴瘤的预后比继发性淋巴瘤好。淋巴瘤的组织学分型与疾病表现和预后高度相关。
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引用次数: 0
Promotive role of eukaryotic translation initiation factor 4A isoform 3 in ovarian cancer cell growth and aerobic glycolysis through the pyruvate dehydrogenase kinase 4 signaling. 真核翻译起始因子4A异构体3通过丙酮酸脱氢酶激酶4信号通路促进卵巢癌细胞生长和有氧糖酵解的作用
IF 3.3 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2023-05-01 DOI: 10.1002/kjm2.12690
Sha-Sha Bai, Li-Wei Yan, Chun-Hui Liu

Ovarian cancer (OC) represents one of the most detrimental gynecological malignancies. RNA-binding protein eukaryotic translation initiation factor 4A isoform 3 (EIF4A3) is well-regarded as a definitive oncogene that contributes to the development of multiple malignant tumors. This study sought to elucidate the molecular mechanism of EIF4A3 in OC growth and aerobic glycolysis by regulation of pyruvate dehydrogenase kinase 4 (PDK4) mRNA stability. We determined the EIF4A3 and PDK4 expression levels in OC cell lines and normal ovarian epithelial cells, and subsequently evaluated the cell viability and colony formation by cell counting kit-8 and colony formation assays. The degree of cell aerobic glycolysis was evaluated by measurements of lactic acid production, glucose intake, adenosine triphosphate level, extracellular oxygen consumption, and protein levels of pyruvate kinase isozymes M2 and hexokinase-2. Afterwards, we verified the binding of EIF4A3 and PDK4 mRNA via RNA immunoprecipitation, and determined the mRNA stability after actinomycin D treatment. Finally, a series of rescue experiments was performed with pcDNA3.1-PDK4. EIF4A3 and PDK4 were upregulated in OC cells. Silencing EIF4A3 obstructed cell proliferation and aerobic glycolysis, while the same was annulled by EIF4A3 overexpression. Mechanically, EIF4A3 could bind to PDK4 mRNA to stabilize its mRNA and upregulate its protein levels. PDK4 overexpression inverted the inhibitory role of silencing EIF4A3 in proliferation and aerobic glycolysis. Overall, our findings highlighted that EIF4A3 induced OC progression by stabilizing PDK4 mRNA.

卵巢癌(OC)是最有害的妇科恶性肿瘤之一。rna结合蛋白真核生物翻译起始因子4A异构体3 (EIF4A3)被认为是促进多发性恶性肿瘤发展的决定性癌基因。本研究旨在通过调节丙酮酸脱氢酶激酶4 (PDK4) mRNA的稳定性来阐明EIF4A3在OC生长和有氧糖酵解中的分子机制。我们检测了EIF4A3和PDK4在OC细胞系和正常卵巢上皮细胞中的表达水平,随后通过细胞计数试剂盒-8和集落形成实验评估了细胞活力和集落形成。通过测量乳酸生成、葡萄糖摄入量、三磷酸腺苷水平、细胞外耗氧量以及丙酮酸激酶同工酶M2和己糖激酶-2的蛋白质水平来评估细胞有氧糖酵解的程度。随后,我们通过RNA免疫沉淀验证了EIF4A3与PDK4 mRNA的结合,并测定了放线菌素D处理后mRNA的稳定性。最后用pcDNA3.1-PDK4进行一系列的抢救实验。EIF4A3和PDK4在OC细胞中上调。沉默EIF4A3会阻碍细胞增殖和有氧糖酵解,而过表达EIF4A3则会消除这种影响。机械上,EIF4A3可以结合PDK4 mRNA以稳定其mRNA并上调其蛋白水平。PDK4过表达逆转了沉默EIF4A3对细胞增殖和有氧糖酵解的抑制作用。总的来说,我们的研究结果强调了EIF4A3通过稳定PDK4 mRNA诱导OC进展。
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引用次数: 1
Lavender essential oil accelerates lipopolysaccharide-induced chronic wound healing by inhibiting caspase-11-mediated macrophage pyroptosis. 薰衣草精油通过抑制caspase-11介导的巨噬细胞凋亡来加速脂多糖诱导的慢性伤口愈合。
IF 3.3 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2023-05-01 DOI: 10.1002/kjm2.12654
Xiang Ao, Huan Yan, Mei Huang, Wei Xing, Luo-Quan Ao, Xiao-Feng Wu, Cheng-Xiu Pu, Bao-Yue Zhang, Xiang Xu, Hua-Ping Liang, Wei Guo

Chronic wounds seriously affect the quality of life of the elderly, obese people, and diabetic patients. The excessive inflammatory response is a key driver of delayed chronic wound healing. Although lavender essential oil (EO [lav]) has been proven to have anti-inflammatory and accelerate wound curative effects, the specific molecular mechanism involved is still ambiguous. The results showed that the wounds treated with lipopolysaccharide (LPS) not only had delayed healing, but also the expression levels of pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), and the inflammatory mediator protein, high-mobility group box 1 protein (HMGB-1), in the wound tissues were significantly increased. However, treatment of LPS-induced chronic wounds with EO (lav) accelerated wound healing and decreased IL-1β and HMGB-1 expression levels. It was further found that LPS induced macrophage pyroptosis to produce IL-1β. After treatment with EO (lav), the expression level of macrophage pyroptosis marker Gasdermin D (GSDMD) and pyroptosis-related cytotoxic effects were significantly reduced. Immunofluorescence results also directly indicate that EO (lav) can protect macrophages from LPS-induced pyroptosis. Moreover, EO (lav) can down-regulate expression levels of IL-1β, GSDMD, and nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) in the caspase-11-related pyroptotic signaling pathway. This study demonstrates that EO (lav) can reduce proinflammatory factor production and ameliorate inflammatory response by inhibiting macrophage pyroptosis, which accelerates LPS-induced chronic wound healing.

慢性伤口严重影响老年人、肥胖者和糖尿病患者的生活质量。过度的炎症反应是延迟慢性伤口愈合的关键驱动因素。虽然薰衣草精油(EO [lav])已被证明具有抗炎和加速伤口愈合的作用,但其具体的分子机制仍不明确。结果表明,脂多糖(LPS)处理的创面不仅愈合延迟,而且促炎因子如肿瘤坏死因子-α (TNF-α)、白细胞介素-6 (IL-6)、白细胞介素-1β (IL-1β)及炎症介质蛋白高迁移率组蛋白1 (HMGB-1)的表达水平均显著升高。然而,用EO (lav)治疗lps诱导的慢性伤口可加速伤口愈合,降低IL-1β和HMGB-1表达水平。进一步发现LPS诱导巨噬细胞热亡产生IL-1β。经EO (lav)处理后,巨噬细胞热亡标志物气凝胶蛋白D (GSDMD)的表达水平和热亡相关的细胞毒作用均显著降低。免疫荧光结果也直接表明EO (lav)对lps诱导的巨噬细胞焦亡具有保护作用。此外,EO (lav)可以下调caspase-11相关焦亡信号通路中IL-1β、GSDMD和核苷酸结合寡聚结构域样受体蛋白3 (NLRP3)的表达水平。本研究表明,EO (lav)可以通过抑制巨噬细胞焦亡,减少促炎因子的产生,改善炎症反应,从而加速lps诱导的慢性伤口愈合。
{"title":"Lavender essential oil accelerates lipopolysaccharide-induced chronic wound healing by inhibiting caspase-11-mediated macrophage pyroptosis.","authors":"Xiang Ao,&nbsp;Huan Yan,&nbsp;Mei Huang,&nbsp;Wei Xing,&nbsp;Luo-Quan Ao,&nbsp;Xiao-Feng Wu,&nbsp;Cheng-Xiu Pu,&nbsp;Bao-Yue Zhang,&nbsp;Xiang Xu,&nbsp;Hua-Ping Liang,&nbsp;Wei Guo","doi":"10.1002/kjm2.12654","DOIUrl":"https://doi.org/10.1002/kjm2.12654","url":null,"abstract":"<p><p>Chronic wounds seriously affect the quality of life of the elderly, obese people, and diabetic patients. The excessive inflammatory response is a key driver of delayed chronic wound healing. Although lavender essential oil (EO [lav]) has been proven to have anti-inflammatory and accelerate wound curative effects, the specific molecular mechanism involved is still ambiguous. The results showed that the wounds treated with lipopolysaccharide (LPS) not only had delayed healing, but also the expression levels of pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), and the inflammatory mediator protein, high-mobility group box 1 protein (HMGB-1), in the wound tissues were significantly increased. However, treatment of LPS-induced chronic wounds with EO (lav) accelerated wound healing and decreased IL-1β and HMGB-1 expression levels. It was further found that LPS induced macrophage pyroptosis to produce IL-1β. After treatment with EO (lav), the expression level of macrophage pyroptosis marker Gasdermin D (GSDMD) and pyroptosis-related cytotoxic effects were significantly reduced. Immunofluorescence results also directly indicate that EO (lav) can protect macrophages from LPS-induced pyroptosis. Moreover, EO (lav) can down-regulate expression levels of IL-1β, GSDMD, and nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) in the caspase-11-related pyroptotic signaling pathway. This study demonstrates that EO (lav) can reduce proinflammatory factor production and ameliorate inflammatory response by inhibiting macrophage pyroptosis, which accelerates LPS-induced chronic wound healing.</p>","PeriodicalId":49946,"journal":{"name":"Kaohsiung Journal of Medical Sciences","volume":"39 5","pages":"511-521"},"PeriodicalIF":3.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9435295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Matrine regulates Th1/Th2 inflammatory responses by inhibiting the Hsp90/NF-κB signaling axis to alleviate atopic dermatitis. 苦参碱通过抑制Hsp90/NF-κB信号轴调节Th1/Th2炎症反应,减轻特应性皮炎。
IF 3.3 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2023-05-01 DOI: 10.1002/kjm2.12655
Pan Huang, Fan Hu, Zhi-Bo Yang, Yi Pan, Rong Zhou, Yi-Ning Yan, Hai-Zhen Wang, Chang Wang

Atopic dermatitis (AD) is a common inflammatory skin disease. Matrine is the main component of the traditional Chinese medicine Sophora flavescens, and it poses good therapeutic effects on inflammatory diseases. This study aimed to explore the pharmacological effects of matrine on AD and its underlying mechanism. An AD mouse model and inflamed human epidermal keratinocyte cells (HaCaT) cells were established. Histopathological aspects were examined using hematoxylin and eosin staining, toluidine blue staining, and immunohistochemistry. The mRNA and protein expressions were assessed using quantitative real-time polymerase chain reaction and Western blot, respectively. The secretions of cytokines and chemokines were examined by enzyme-linked immunosorbent assay. Flow cytometry was carried out to analyze the proportions of T-helper (Th) 1 and Th2 cells. Herein, our results displayed that matrine diminished AD symptoms and decreased heat shock protein 90 (Hsp90) expression. Matrine decreased the Th2 cytokine levels in the ear tissues and serum, and it also significantly repressed inflammatory cytokines (thymus activation regulated chemokine and interleukin-6) secretions by repressing the Hsp90/NF-κB signaling axis in inflamed HaCaT cells. Furthermore, matrine inhibited Th2 differentiation of CD4+ T cells when co-cultured with inflamed HaCaT cells. Matrine can regulate the Th1/Th2 inflammatory response by inhibiting the Hsp90/NF-κB signaling axis to alleviate AD. Therefore, it may be a candidate for AD treatment.

特应性皮炎(AD)是一种常见的炎症性皮肤病。苦参碱是中药苦参的主要成分,对炎症性疾病有很好的治疗作用。本研究旨在探讨苦参碱对AD的药理作用及其机制。建立AD小鼠模型和人表皮角质形成细胞(HaCaT)。组织病理学方面采用苏木精和伊红染色,甲苯胺蓝染色和免疫组织化学检查。分别采用实时定量聚合酶链反应和Western blot检测mRNA和蛋白的表达。酶联免疫吸附法检测细胞因子和趋化因子的分泌。流式细胞术检测t -辅助性(Th) 1和Th2细胞比例。本研究结果显示,苦参碱可以减轻AD症状,降低热休克蛋白90 (Hsp90)的表达。苦参碱降低耳组织和血清中Th2细胞因子水平,并通过抑制HaCaT炎症细胞Hsp90/NF-κB信号轴显著抑制炎症细胞因子(胸腺活化调节趋化因子和白细胞介素-6)的分泌。此外,当与炎症HaCaT细胞共培养时,苦参碱抑制CD4+ T细胞的Th2分化。苦参碱可通过抑制Hsp90/NF-κB信号轴调节Th1/Th2炎症反应,减轻AD。因此,它可能是治疗阿尔茨海默病的候选药物。
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引用次数: 3
Robotic arm use for upper limb rehabilitation after stroke: A systematic review and meta-analysis. 机械臂用于中风后上肢康复:一项系统回顾和荟萃分析。
IF 3.3 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2023-05-01 DOI: 10.1002/kjm2.12679
Bih-O Lee, Ita Daryanti Saragih, Sakti Oktaria Batubara

Several studies have reported the effects of robotic arms on improving upper limb function in patients with stroke. However, previous studies have reported inconsistent findings that may lead to incorrect applications of robotic arm use. Six databases were searched for relevant randomized controlled trials. Meta-analyses were performed for upper limb performance measures, including subgroup analysis of pooled upper limb rehabilitation data such as stroke stage and intervention delivery dose. Furthermore, the Cochrane risk-of-bias tool for randomized trials version 2 (RoB 2) and sensitivity analysis were used to assess methodology and determine publication bias. The final analysis included 18 studies. Robotic arms improved upper limb and hand function in patients with stroke. Subgroup analysis revealed that robotic arm interventions lasting 30-60 min per session significantly improved upper limb function. However, no significant improvement was observed in shoulder and elbow or wrist and hand movements. This review may help develop applicable rehabilitation robots and collaboration between clinicians.

一些研究报道了机械臂对改善中风患者上肢功能的影响。然而,先前的研究报告了不一致的发现,这可能导致机械臂使用的不正确应用。在6个数据库中检索相关的随机对照试验。对上肢表现指标进行meta分析,包括合并上肢康复数据的亚组分析,如卒中分期和干预给药剂量。此外,采用Cochrane随机试验风险偏倚工具第2版(RoB 2)和敏感性分析来评估方法学和确定发表偏倚。最终的分析包括18项研究。机械臂改善了中风患者的上肢和手部功能。亚组分析显示,每次持续30-60分钟的机械臂干预可显著改善上肢功能。然而,肩、肘、腕和手的运动没有明显的改善。这一综述可能有助于开发适用的康复机器人和临床医生之间的合作。
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引用次数: 1
Obstructive jaundice caused by IgG4-related disease with multiorgan involvement. 多器官累及igg4相关疾病引起的梗阻性黄疸
IF 3.3 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2023-05-01 DOI: 10.1002/kjm2.12668
Gwo-Kuang Yu, Shen-Nien Wang
Immunoglobulin G4-related disease (IgG4-RD) is an immune-mediated disease with multiorgan involvement. The variable clinical manifestations and image findings of this disease often present a diagnostic dilemma for physicians. Herein, we describe a patient with IgG4-RD involving the kidney, retroperitoneum, pancreas, and biliary tracts, successfully treated with steroids. A 68-year-old man presented to our emergency room with a 2-week history of jaundice, tea-color urine, and poor appetite. He denied presence of fever, dyspnea, abdominal pain, or dysuria recently. Relevant laboratory data showed elevated liver enzymes and bilirubinemia (SGOT = 82 IU/L, SGPT = 174 IU/L, ALP = 196 IU/L, GGT = 340 IU/L, total bilirubin = 12 mg/dl, direct bilirubin = 7.51 mg/dl) with normal C-Reactive protein (CRP), blood cell counts and renal function. Abdominal computed tomography (CT) showed enhancing soft tissue lesions at bilateral renal pelvises (Figure 1A, arrow) and paraaortic regions (Figure 1B, arrow), diffuse swelling of the pancreas with a peripheral poorly enhanced halo (Figure C, short arrow) and dilatation of intra-hepatic and extra-hepatic bile ducts associated with enhancing wall thickenings (Figure 1 D, arrow). Urine cytology, performed twice, was negative for malignant cells. Ureterorenoscopy
{"title":"Obstructive jaundice caused by IgG4-related disease with multiorgan involvement.","authors":"Gwo-Kuang Yu,&nbsp;Shen-Nien Wang","doi":"10.1002/kjm2.12668","DOIUrl":"https://doi.org/10.1002/kjm2.12668","url":null,"abstract":"Immunoglobulin G4-related disease (IgG4-RD) is an immune-mediated disease with multiorgan involvement. The variable clinical manifestations and image findings of this disease often present a diagnostic dilemma for physicians. Herein, we describe a patient with IgG4-RD involving the kidney, retroperitoneum, pancreas, and biliary tracts, successfully treated with steroids. A 68-year-old man presented to our emergency room with a 2-week history of jaundice, tea-color urine, and poor appetite. He denied presence of fever, dyspnea, abdominal pain, or dysuria recently. Relevant laboratory data showed elevated liver enzymes and bilirubinemia (SGOT = 82 IU/L, SGPT = 174 IU/L, ALP = 196 IU/L, GGT = 340 IU/L, total bilirubin = 12 mg/dl, direct bilirubin = 7.51 mg/dl) with normal C-Reactive protein (CRP), blood cell counts and renal function. Abdominal computed tomography (CT) showed enhancing soft tissue lesions at bilateral renal pelvises (Figure 1A, arrow) and paraaortic regions (Figure 1B, arrow), diffuse swelling of the pancreas with a peripheral poorly enhanced halo (Figure C, short arrow) and dilatation of intra-hepatic and extra-hepatic bile ducts associated with enhancing wall thickenings (Figure 1 D, arrow). Urine cytology, performed twice, was negative for malignant cells. Ureterorenoscopy","PeriodicalId":49946,"journal":{"name":"Kaohsiung Journal of Medical Sciences","volume":"39 5","pages":"546-547"},"PeriodicalIF":3.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9490842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of linked color imaging and blue laser imaging on the diagnosis of esophageal squamous cell carcinoma in iodine unstained areas. 彩色和蓝色激光联合成像对碘未染色区食管鳞状细胞癌诊断的影响。
IF 3.3 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2023-05-01 DOI: 10.1002/kjm2.12660
Masato Tsunoda, Yoshimasa Miura, Hiroyuki Osawa, Manabu Nagayama, Yuka Kagaya, Yohei Funayama, Takuma Kobayashi, Mami Togashi, Hiroki Hayashi, Yuji Hiraoka, Yoshie Nomoto, Chihiro Iwashita, Yuji Ino, Haruo Takahashi, Hisashi Fukuda, Alan Kawarai Lefor, Hironori Yamamoto

The pink color sign in iodine unstained areas is useful to differentiate esophageal squamous cell carcinoma (ESCC) from other lesions. However, some ESCCs have obscure color findings which affect the ability of endoscopists to differentiate these lesions and determine the resection line. Using white light imaging (WLI), linked color imaging (LCI) and blue laser imaging (BLI), 40 early ESCCs were retrospectively evaluated using images before and after iodine staining. Visibility scores for ESCC by expert and non-expert endoscopists were compared using these three modalities and color differences measured for malignant lesions and surrounding mucosa. BLI had the highest score and color difference without iodine staining. Each determination with iodine was much higher than without iodine regardless of the modality. With iodine, ESCC mainly appeared pink, purple and green using WLI, LCI and BLI, respectively and visibility scores determined by non-experts and experts were significantly higher for LCI (both p < 0.001) and BLI (p = 0.018 and p < 0.001) than for WLI. The score with LCI was significantly higher than with BLI among non-experts (p = 0.035). With iodine, the color difference using LCI was twice that with WLI and one with BLI was significantly larger than with WLI (p < 0.001). These greater tendencies were found regardless of location, depth of cancer or intensity of pink color using WLI. In conclusion, areas of ESCC unstained by iodine were easily recognized using LCI and BLI. Visibility of these lesions is excellent even by non-expert endoscopists, suggesting that this method is useful to diagnose ESCC and determine the resection line.

未染碘区的粉红色征象有助于区分食管鳞状细胞癌(ESCC)和其他病变。然而,一些escc有模糊的颜色发现,这影响了内镜医师区分这些病变和确定切除线的能力。采用白光成像(WLI)、联色成像(LCI)和蓝光成像(BLI)对40例早期escc进行回顾性评价。专家和非专家内窥镜医师对ESCC的可见性评分使用这三种方式和恶性病变和周围粘膜的颜色差异进行比较。无碘染色BLI评分最高,色差最大。无论采用何种方式,加碘的各项测定结果均明显高于不加碘。对于碘,使用WLI、LCI和BLI, ESCC分别主要呈现粉红色、紫色和绿色,并且LCI的非专家和专家确定的可见性得分显著更高(p
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引用次数: 1
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Kaohsiung Journal of Medical Sciences
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