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A framework for limited use of high-dose areas following the Fukushima nuclear accident. 福岛核事故后高剂量地区有限使用的框架。
IF 1.8 4区 环境科学与生态学 Q4 ENVIRONMENTAL SCIENCES Pub Date : 2026-03-23 DOI: 10.1088/1361-6498/ae4c9d
Yoshika Saito, Naomi Ito, Toshiki Abe, Chika Yamamoto, Chihiro Matsumoto, Tianchen Zhao, Nobuaki Moriyama, Kazuya Yoshimura, Yukihisa Sanada, Masaharu Tsubokura

This study analysed Japan's implementation of a regulatory framework that enables limited, non-residential land use in areas where radiation levels remain too high for repopulation following a nuclear accident, focusing on a wind power project in Katsurao Village, Fukushima Prefecture. Although international initiatives have advanced protective actions for the emergency phase of radiological events, comparatively little guidance exists for the recovery phase, particularly regarding procedures for lifting evacuation orders in persistently high-dose areas. Japan's experience is unique: while evacuation orders have been lifted across multiple municipalities, some zones remain unsuitable for habitation, necessitating new policy approaches. To address this gap, Katsurao Village employed the framework to authorise land use in a 19.9 hectare district designated for wind power generation. A multi-stakeholder committee-comprising residents, experts, government authorities, and industry representatives-was established to evaluate dose-reduction measures, access protocols, environmental maintenance, and operational safeguards. The committee identified several unresolved issues, including the absence of standardised monitoring procedures, challenges in managing worker exposure, the need for clear demarcation and communication to prevent unauthorised entry, and mechanisms for transparent reporting in the event of abnormal findings. This case demonstrates both the feasibility and complexity of enabling controlled land use in high-dose areas, underscoring the importance of governance, technical standardisation, and sustained risk communication.

本研究分析了日本监管框架的实施情况,该框架允许在核事故后辐射水平仍然过高的地区有限地使用非住宅土地,从而无法重新人口,重点研究了福岛县Katsurao村的一个风力发电项目。虽然国际倡议已经为辐射事件的紧急阶段采取了先进的保护行动,但对于恢复阶段的指导相对较少,特别是关于在持续高剂量地区解除疏散令的程序。日本的经验是独特的:虽然多个城市解除了疏散令,但一些地区的辐射剂量继续超过适宜居住的水平,需要采取新的政策措施。为了解决这一差距,Katsurao村采用了该框架,授权在一个19.9公顷的地区使用土地,指定用于风力发电。建立了一个由居民、专家、政府当局和行业代表组成的多利益攸关方委员会,以评估减少剂量措施、获取协议、环境维护和操作保障措施。委员会确定了几个尚未解决的问题,包括缺乏标准化的监测程序、管理工人接触的挑战、需要明确的界限和沟通以防止未经授权的进入,以及在发现异常情况时透明报告的机制。这个案例展示了在高剂量地区控制土地使用的可行性和复杂性,强调了治理、技术标准化和持续风险沟通的重要性。
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引用次数: 0
CORRIGENDUM: Assessment of population exposure to terrestrial gamma radiation in South Korea (2025J. Radiol. Prot. 43 031506). 勘误:评估韩国人口对地面伽玛辐射的暴露(2025年)。prof . 43 031506)。
IF 1.8 4区 环境科学与生态学 Q4 ENVIRONMENTAL SCIENCES Pub Date : 2026-03-23 DOI: 10.1088/1361-6498/ae519a
Jaewoo Park, Yong-Jae Kim, Byung-Uck Chang, Jaeho Jang, Kwang Pyo Kim, Ji-Young Kim
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引用次数: 0
Mortality among workers at the Rocky Flats Plant, 1951-2017. Rocky Flats工厂工人死亡率,1951-2017。
IF 1.8 4区 环境科学与生态学 Q4 ENVIRONMENTAL SCIENCES Pub Date : 2026-03-23 DOI: 10.1088/1361-6498/ae4d57
Sara C Howard, Michael T Mumma, Caleigh E Samuels, Michael B Bellamy, Rich W Leggett, Keith F Eckerman, Elizabeth D Ellis, Lawrence T Dauer, Ashley P Golden

The Rocky Flats (RFs) Plant operated from 1951-1989 as part of the U.S. Department of Energy (DOE) nuclear complex. Its primary mission was weapons component fabrication, whereby workers were potentially exposed to radioactive and non-radioactive hazards. RF worker mortality was compared to the general population, and dose-response relationships between mortality and radiation organ doses were examined. RF workers first employed between 1951 and 1979 for ⩾30 d were identified (n= 9397). Vital status was determined using national and state death records up to 2017. Organ doses from external photons and neutrons irritation and internalised plutonium (Pu), americium (Am), and uranium (U) were modelled as cumulative lagged total doses per year. Beryllium exposure was evaluated as an effect modifier using data from the DOE Nationwide Beryllium Medical Program. Statistical analyses included standardised mortality ratios (SMRs), Cox proportional hazard models, and excess relative risk (ERR) models. Approximately 53.2% of workers were deceased by the end of the study. Nearly 90% were monitored for radiation exposure, with a mean weighted absorbed dose of 59.0 mGy for the lungs. Nearly 45% of workers had intakes of alpha-particle emitting radionuclides, and 46.7% were monitored for neutrons. Leading causes of death included ischemic heart disease (n= 999) and lung cancer (n= 361). The highest SMRs were observed for berylliosis (SMR: 176.9; 95% CI: 76.2, 348.7;n< 10) and asbestosis (SMR: 4.65; 95% CI: 2.23, 8.55;n= 10). Dose-response analyses showed no statistical increase in risk from low-dose radiation including lung cancer (ERR per 100 mGy: -0.02; 95% CI: -0.11, 0.08;n= 361) and Parkinson's disease (ERR per 100 mGy: 0.13; 95% CI: -0.26, 0.31;n= 57). Approximately 45% of workers were monitored for beryllium, with a weak non-significant indication of effect modification for lung cancer risk. The RF cohort showed no evidence of a statistically significant increase in mortality from occupational radiation exposure. However, this study was limited by low statistical power, which inhibits the ability to detect effects. Future pooling of Million Person Study (MPS) cohorts will provide further insights, particularly regarding Pu as a carcinogen.

背景:Rocky Flats (RF)电厂作为美国能源部(DOE)核设施的一部分,从1951年到1989年运行。它的主要任务是制造武器部件,因此工人可能暴露在放射性和非放射性危害中。将射频工作人员的死亡率与一般人群进行比较,并检查死亡率与辐射器官剂量之间的剂量-反应关系。方法:确定1951年至1979年间首次就业≥30天的射频工人(n=9,397)。生命状态是根据截至2017年的国家和州死亡记录确定的。外部光子和中子照射和内化钚、镅和铀的器官剂量被建模为每年累积滞后总剂量。根据美国能源部全国铍医疗计划的数据,评估了铍暴露作为影响调节剂的作用。统计分析包括标准化死亡率(SMRs)、Cox比例风险模型和过度相对风险(ERR)模型。结果:约53.2%的患者在研究结束时死亡。近90%的患者接受了辐射暴露监测,肺部平均加权吸收剂量为59.0 mGy。近45%的工人摄入了释放放射性核素的α粒子,46.7%的工人接受了中子监测。主要死亡原因包括缺血性心脏病(n=999)和肺癌(n=361)。青光板病的SMR最高(SMR: 176.9; 95% CI: 76.2, 348.7)。结论:RF队列显示,职业性辐射暴露的死亡率没有统计学上显著增加的证据。然而,这项研究受到低统计能力的限制,这抑制了检测效果的能力。未来对MPS队列的汇集将提供进一步的见解,特别是关于钚作为致癌物的见解。
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引用次数: 0
Launching Japan's first collaborative model for implementing shelter-in-place operations at medical institutions near nuclear power plants. 启动日本首个协作模式,在核电站附近的医疗机构实施就地避难操作。
IF 1.8 4区 环境科学与生态学 Q4 ENVIRONMENTAL SCIENCES Pub Date : 2026-03-23 DOI: 10.1088/1361-6498/ae4b48
Chika Yamamoto, Chihiro Matsumoto, Shota Tanabe, Hideyuki Kato, Shinya Fukasawa, Tianchen Zhao, Yuji Yamamori, Toyoaki Sawano, Saori Nonaka, Naoyuki Yamamoto, Arifumi Hasegawa, Nobuyuki Hirohashi, Masato Homma, Tsubokura Masaharu

Shelter-in-place (SIP) is recognised alongside evacuation as a primary protective action during nuclear emergencies in Japan. Following the Fukushima Daiichi Nuclear Power Plant accident, increasing recognition of evacuation-related health risks-particularly among hospitalised and institutionalised populations-has strengthened policy emphasis on SIP within the urgent protective action planning zone (UPZ). However, little attention has been paid to the practical implementation of SIP in medical institutions. Hospitals face distinctive operational challenges during SIP, including business continuity planning (BCP), radiation protection, staff mobilisation, internal zoning, and the operation of positive-pressure systems. Despite widespread installation of such systems, their operational feasibility during nuclear emergencies remains largely unexplored. This study reports Japan's first implementation-oriented effort to operationalize SIP within a hospital setting. Since fiscal year 2024, a nuclear disaster preparedness working group has been established at Japanese Red Cross Matsue Hospital, a designated disaster base hospital located within the UPZ of the Shimane Nuclear Power Plant. Through staged departmental reviews and multidisciplinary discussions involving hospital staff and external stakeholders, operational challenges were systematically identified and incorporated into the hospital's BCP. By addressing the gap between SIP policy and operational feasibility, this study provides practical evidence to support nuclear emergency preparedness in medical institutions within UPZs.

在日本发生核紧急情况时,就地避难(SIP)是与疏散并列的主要保护措施。然而,尽管制度制度和指导方针的逐步发展,但对医疗机构SIP的实际实施的关注有限。特别是,必须继续为老年人、危重病人和其他无法撤离的住院病人提供护理的医院,缺乏解决业务连续性规划(BCP)、辐射防护、员工动员、分区和正压系统实际操作的综合运营模式。在此背景下,自2024财政年度以来,日本红十字会松江医院成立了一个核灾难准备工作组,该医院是位于岛根核电站紧急保护行动规划区内的指定灾害基地医院。该工作组汇集了多学科医院部门和外部利益攸关方,包括地方政府、相关服务提供者和学术机构,并在一个永久性的多层治理结构下运作。通过在各部门内分阶段进行筹备审查和在全体会议上进行综合讨论,系统地确定了与SIP有关的业务挑战,并将其纳入医院级BCP。这一举措是日本首次以实施为导向的努力,不仅将SIP作为一项概念或行政措施,而且将其作为一项基于现实世界医院实践的业务战略进行审查。通过提高upz内医疗机构核应急准备的可行性,本研究提供了可为未来政策制定提供信息的实践见解,并可适用于其他地区。
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引用次数: 0
Electric field strengths and current densities induced inside the body due to exposure to Electronic Article Surveillance (EAS) system antennas compared to ICNIRP's exposure limits. 暴露于电子物品监视(EAS)系统天线与ICNIRP暴露限值的比较,得出体内感应的电场强度和电流密度。
IF 1.8 4区 环境科学与生态学 Q4 ENVIRONMENTAL SCIENCES Pub Date : 2026-03-20 DOI: 10.1088/1361-6498/ae553b
Pia Schneeweiss, Rene Hirtl, Tobias Jhala, Richard Ueberbacher, Stefan Cecil, Corinna Becker, Ingo Boemmels, Carsten Altekoester, Gernot Schmid

Personal exposure to magnetic fields emitted by electronic article surveillance (EAS) systems was systematically assessed based on measurements of a representative sample of 19 different EAS devices. This sample included the two major EAS technologies currently on the market: acoustomagnetic (AM) and radio frequency (RF) systems. In addition to these measurements, numerical computations of the current densities and electric field strengths induced in body tissues were carried out for one representative AM-EAS device and several body models (adult male, adult female, child female, and a hand model) and reasonably foreseeable worst-case exposure scenarios using a real-valued magneto quasi-static solver, based on the Scalar Potential Finite Element (SPFE) method. The obtained measurement and computational results were compared to the different sets of exposure limits defined by the International Commission for Non-Ionizing Radiation Protection (ICNIRP) in 1998 and 2010. Our results demonstrated that current RF-EAS technology, which typically operates in the 8.2 MHz frequency range, does not conflict with the exposure limits, even under adverse exposure conditions. However, AM-EAS systems, which typically operate at 58 kHz, may lead to induced current densities in the central nervous system and induced electric field strengths in the peripheral nervous systems of adults and children that exceed the basic restrictions for the general public (up to a factor of 22.5 for the adult female bending in front of the antenna) and even for occupational exposure (up to a factor of 4.5 for the same scenario) according to the ICNIRP 1998 and 2010 guidelines under reasonably foreseeable exposure conditions, which are not covered by the assessment procedures defined in the present version of the applicable standard IEC EN 62369-1. Therefore, radiation protection and market regulatory authorities should have a close look and check presently installed and future AM-EAS technology with respect to their compliance to exposure limits.

基于19种不同的电子物品监控(EAS)设备的代表性样本的测量,系统地评估了个人暴露于电子物品监控(EAS)系统发出的磁场。该样本包括目前市场上的两种主要EAS技术:声磁(AM)和射频(RF)系统。此外,基于标量势有限元(SPFE)方法,采用实值磁致准静态求解器对一个具有代表性的AM-EAS装置和多个人体模型(成年男性、成年女性、儿童女性和手部模型)进行了电流密度和人体组织中诱发的电场强度的数值计算,并合理预测了最坏情况下的暴露情况。将获得的测量和计算结果与国际非电离辐射防护委员会(ICNIRP)在1998年和2010年确定的不同暴露限值进行了比较。我们的研究结果表明,目前的RF-EAS技术通常工作在8.2 MHz频率范围内,即使在不利的暴露条件下,也不会与暴露限制相冲突。然而,AM-EAS系统通常工作在58千赫,可能导致中枢神经系统的感应电流密度和成人和儿童周围神经系统的感应电场强度超过一般公众的基本限制(成年女性在天线前弯曲的系数高达22.5),甚至超过职业暴露(根据ICNIRP 1998年和2010年指南,在合理可预见的暴露条件下,相同情况下的系数高达4.5)。在适用标准IEC EN 62369-1的当前版本中定义的评估程序中未涵盖的。因此,辐射防护和市场监管机构应该密切关注并检查目前安装的和未来的AM-EAS技术是否符合暴露限值。
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引用次数: 0
European radiation protection week 2025-meeting summary. 2025年欧洲辐射防护周会议纪要。
IF 1.8 4区 环境科学与生态学 Q4 ENVIRONMENTAL SCIENCES Pub Date : 2026-03-20 DOI: 10.1088/1361-6498/ae506b
Simon Bouffler, Francesca Antonelli, Christophe Badie, Stephen Barnard, Amy Berrington de Gonzalez, Antony Bexon, Sharon Deacon, Ämilie Degenhardt, Louise Elstow, Chloe Giles, Anastasia Ioakeimidou, Alison Jones, Eymeric Lafranque, Vijaya Lillyjanet, Hannah Mancey, Karen Marchant, Veronika Olšovcová, Francois Paris, Almudena Real, Katrine Riklund, Yannick Saintingy, Rick Tanner, Max Taylor, Sebastian Trinkl, Yevgeniya Tomkiv, Filip Vanhavere, Christine Werner, Kerry Whiteway, Kinga Zmijewska, Liz Ainsbury

From 29th September to 2nd October 2025, European Radiation Protection Week was hosted in London by the UK Health Security Agency with Imperial College London's Department of Epidemiology and Biostatistics. The meeting brought together the platforms under the MEENAS umbrella (MELODI, EURADOS, EURAMED, NERIS, ALLIANCE, and SHARE) to deliver a diverse programme of presentations and posters spanning the breadth of radiation protection research. This paper provides a summary of the meeting.

欧洲辐射防护周(ERPW)现已成为从事辐射防护研究的人的活动日历中的一个固定特色。第一次这样的活动于2016年在英国牛津大学数学研究所举办,英国公共卫生部(现为英国卫生安全局)是主要组织者(Bouffler, 2017)。2025年是ERPW的第九个年头,将于2025年9月29日至10月2日在伦敦举行,由英国卫生安全局与伦敦帝国理工学院流行病学和生物统计学部门领导该组织。会议在伦敦西区靠近大理石拱门的坎伯兰酒店举行,会议汇集了一个多元化和引人入胜的计划,涵盖了MEENAS伞型组织(https://www.meenas.eu/)下联合的欧洲平台所涵盖的所有辐射防护研究领域:MELODI, EURADOS, EURAMED, NERIS, ALLIANCE和SHARE。按照惯例,第一天包括一系列卫星会议(整个活动共9次),随后是为期三天的主要方案。会议形式包括全体会议、并行会议和海报展示,以及贸易展览。这篇简短的文章提供了为期三天的核心活动的摘要。
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引用次数: 0
Interim results of EC project RRADEW: Resilience to RADiological events in wartime. 欧共体项目rraddew的中期结果:战时对辐射事件的恢复能力。
IF 1.8 4区 环境科学与生态学 Q4 ENVIRONMENTAL SCIENCES Pub Date : 2026-03-20 DOI: 10.1088/1361-6498/ae553c
Pascal Crouail, Eymeric Lafranque, Thierry Schneider, Olena Pareniuk, Kateryna Shavanova, Anastasiia Torianik, Yuliya Ruban, Sadeeb Simon Ottenburger, Thomas Makumbi, Elsa Gisquet, Yevgeniya Tomkiv, Deborah H Oughton, Friedo Zölzer, Stepan Kavan, Eva- Styblova, Catrinel Turcanu, Gaston Meskens, Robbe Geysmans, Ahmed Nagy, Denis Giordan, Antony Bexon, Paulo Marques Nunes, Christopher Rääf, Milagros Montero, Blanca García Puerta

The context of armed conflict situations presents unique challenges that can compromise the safety and well-being of both affected populations and responders in radiological and nuclear emergencies. The focus of the EU project RRADEW "Resilience to RADiological Events in Wartime" is to enhance nuclear Emergency Preparedness, Response and Recovery (EPR&R) systems by strengthening resilience to potential incidents in the context of war or armed conflict. The project is structured in five workpackages focusing on the development of war scenarios (WP1), identification of resilience dimensions and indicators (WP2 and 3), development of decision-support, training materials and recommendations targeting key stakeholders (WP4), and key ethical questions for radiological protection in the context of armed conflicts (WP5). Together, these efforts aim to provide actionable frameworks and recommendations to ensure the safety and well-being of both affected populations and responders in wartime radiological incidents.

武装冲突局势的背景带来了独特的挑战,可能危及受影响人口以及辐射和核紧急情况响应者的安全和福祉。欧盟“战时辐射事件复原力”项目的重点是通过加强对战争或武装冲突背景下潜在事件的复原力,加强核应急准备、响应和恢复(EPR&R)系统。该项目分为五个工作包,重点是制定战争情景(WP1),确定复原力维度和指标(WP2和3),制定针对关键利益攸关方的决策支持、培训材料和建议(WP4),以及武装冲突背景下辐射防护的关键伦理问题(WP5)。这些努力旨在共同提供可操作的框架和建议,以确保战时辐射事件中受影响人群和响应者的安全和福祉。
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引用次数: 0
Development and effectiveness evaluation of an AR educational tool for scattered radiation visualization in occupational radiation protection. 职业辐射防护散射辐射可视化AR教学工具的研制与效果评价。
IF 1.8 4区 环境科学与生态学 Q4 ENVIRONMENTAL SCIENCES Pub Date : 2026-03-18 DOI: 10.1088/1361-6498/ae53f5
Koki Noguchi, Toshioh Fujibuchi, Donghee Han

Introduction: Occupational radiation exposure among medical staff remains a critical concern, underscoring the importance of effective radiation-protection education. This study aimed to develop and evaluate educational materials incorporating an augmented reality (AR) application to visualize scattered-radiation distributions during radiological procedures.

Methods: The educational program comprised a 20-item true/false quiz, a questionnaire based on the Attention, Relevance, Confidence, and Satisfaction (ARCS) motivational model, and open-ended questions assessing perceived strengths and weaknesses.

Results: Pre-and post-training quiz scores demonstrated an improvement of approximately 10% in the overall correct-response rate, indicating measurable gains in factual knowledge related to scattered radiation and radiation protection. ARCS scores reflected high levels of learner motivation, with mean ratings of 3.98 for Attention, 3.99 for Relevance, 4.06 for Confidence, and 4.08 for Satisfaction on a fivepoint scale. Free-text responses suggested that visualizing scattered radiation with AR facilitated a more concrete understanding of its spatial distribution and the effectiveness of protective measures. However, both ARCS feedback and open-ended comments indicated that the training content was dense and that the exercise workload was perceived as burdensome. Participants also reported usability challenges related to the application interface.

Conclusion: Overall, the findings suggest that the AR-based educational materials can enhance both learner motivation and conceptual understanding of radiation protection, although further refinement of scenario design and interface usability is required to optimize learning efficiency and user experience.

导言:医务人员的职业辐射暴露仍然是一个严重问题,这突出了有效辐射防护教育的重要性。本研究旨在开发和评估包含增强现实(AR)应用的教育材料,以可视化放射治疗过程中的散射辐射分布。方法:教育项目包括一个20题的真假测验,一个基于注意力、相关性、信心和满意度(ARCS)动机模型的问卷,以及评估感知优势和劣势的开放式问题。结果:训练前和训练后的测验成绩显示,总体正确率提高了约10%,表明在散射辐射和辐射防护相关的事实知识方面有了可测量的收获。ARCS分数反映了高水平的学习者动机,在五分制中,注意力的平均评分为3.98,相关性为3.99,信心为4.06,满意度为4.08。自由文本回复表明,利用AR可视化散射辐射有助于更具体地了解其空间分布和防护措施的有效性。然而,ARCS反馈和开放式评论都表明,训练内容密集,运动工作量被认为是繁重的。参与者还报告了与应用程序接口相关的可用性挑战。结论:总体而言,基于ar的教育材料可以增强学习者的学习动机和对辐射防护概念的理解,但需要进一步完善场景设计和界面可用性,以优化学习效率和用户体验。
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引用次数: 0
An overview of background radiation level and spatial distribution of NORM in Bangladesh. 孟加拉国NORM本底辐射水平和空间分布概况。
IF 1.8 4区 环境科学与生态学 Q4 ENVIRONMENTAL SCIENCES Pub Date : 2026-03-17 DOI: 10.1088/1361-6498/ae4d58
Md Ashik Azad Khan Anik, Akm Saiful Islam Bhuian, Mahmoud Mahmoud, Nakahiro Yasuda, Quazi Muhammad Rashed-Nizam

This study presents the first comprehensive spatial analysis of activity concentrations of naturally occurring radioactive material (NORM) and absorbed dose rates across Bangladesh. Utilising a database compiled from 56 published studies and 40 unpublished theses (1990-present), environmental sample data were mapped onto a systematic grid system comprising 2094 cells (8.05 km × 8.05 km) from which measured and unmeasured locations were revealed across the country. Ordinary Kriging interpolation was applied to visualize nationwide distributions. The analysis revealed significant spatial heterogeneity primarily driven by differences in geology, rock structures, and soil types. Activity concentrations of Ra-226, Th-232, and K-40 ranged from 0.01 to 647.5 Bq kg-1, 0.2-1230.0 Bq kg-1, and 0.04-2352.0 Bq kg-1, respectively. Consequently, the absorbed dose rates varied widely from 0.02 to 745.86 nGy h-1, yielding a national average of 64.16 nGy h-1. Furthermore, the gridded mapping exposed severe sampling imbalances: while some localized regions were measured up to 13 times, vast areas remain entirely unsampled. By establishing this pre-operational national baseline, this study successfully distinguishes natural background levels from potential technologically enhanced NORM. The findings provide a critical reference for ongoing environmental monitoring particularly to assess radiation levels around the soon-to-be-operational Rooppur nuclear power plant and offer a strategic framework to guide future targeted sampling in unmeasured regions.

本研究首次对孟加拉国各地天然存在的放射性物质(NORM)活动浓度和吸收剂量率进行了全面的空间分析。利用从56篇已发表的研究和40篇未发表的论文(1990年至今)汇编的数据库,将环境样本数据映射到包含2094个单元(8.05 km × 8.05 km)的系统网格系统中。采用普通克里格插值法可视化全国分布。分析表明,主要由地质、岩石结构和土壤类型的差异驱动的显著的空间异质性。Ra-226、Th-232和K-40活性浓度分别为0.01 ~ 647.5 Bq/kg、0.2 ~ 1230.0 Bq/kg和0.04 ~ 2352.0 Bq/kg。因此,吸收剂量率在0.02至745.86纳基/小时之间变化很大,全国平均水平为64.16纳基/小时。此外,网格化制图暴露出严重的采样不平衡:虽然一些局部区域测量了多达13次,但大片地区仍然完全没有采样。通过建立这个操作前的国家基线,本研究成功地区分了自然背景水平和潜在的技术增强标准(TENORM)。这些发现为正在进行的环境监测提供了重要参考,特别是评估即将投入运营的鲁普尔核电站(RNPP)周围的辐射水平,并为指导未来在未测量地区进行有针对性的采样提供了战略框架。
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引用次数: 0
Transcriptomic and proteomic assessment of radiation injury and dose-rate dependency in white blood cells. 白细胞辐射损伤和剂量率依赖性的转录组学和蛋白质组学评估。
IF 1.8 4区 环境科学与生态学 Q4 ENVIRONMENTAL SCIENCES Pub Date : 2026-03-13 DOI: 10.1088/1361-6498/ae4be9
Sanjeena Subedi, Saadia Khilji, Ngoc Q Vuong, Caitlin Kral, Kelly M Fulton, Isabel Baay, Susan M Twine, Ruth C Wilkins, Andrew Williams, Premkumari Kumarathasan, Carole L Yauk, Vinita Chauhan

Ionizing radiation elicits complex cellular responses that are influenced not only by total dose but also by the rate at which the dose is delivered. Understanding how dose rate modulates molecular outcomes is important for accurate risk assessment. In this study, we apply an integrative multi-omics approach combining transcriptomic and proteomic profiling while adjusting for covariates to investigate how differential dose rates of ionizing radiation alter gene and protein expression in human lymphocytes. Particular emphasis is placed on identifying dose-rate-specific alterations in key molecular pathways. Peripheral blood from 14 healthy donors (8 males, 6 females) was irradiatedex vivowith x-rays at 0.05 Gy min-1(DR1) and 1.0 Gy min-1(DR2) across a dose range from 0 to 6 Gy. Gene expression was assessed using TempO-Seq™, and relative protein abundance was determined by mass spectrometry. Differential expression analysis was conducted using edgeR and limma, adjusting for sex, age, and leukocyte counts (false discovery rate < 0.05). Multi-omics integration was performed using regularised canonical correlation analysis (rCCA) implemented in mixOmics, followed by Reactome pathway enrichment analysis. We identified 2477 and 2612 differentially expressed genes at DR1 and DR2, respectively, and 368 and 386 differentially expressed proteins. To assess dose discrimination, we examined sample separation in the space defined by the average canonical variates from transcriptomic and proteomic datasets using rCCA. Covariate adjustment improved dose discrimination, particularly above 0.5 Gy. Using a correlation cut-off threshold of 0.5 in rCCA, 212 (DR1) and 276 (DR2) highly correlated gene-protein pairs were identified. DR2 exposure was associated with stronger enrichment of stress-related pathways, including unfolded protein response, senescence and oncogenic kinase signalling. In contrast, DR1 induced enrichment of pathways associated with immune engagement, including antigen presentation. At both dose rates, transcriptomic changes highlighted upstream regulatory processes (chromatin modelling) and proteomic changes captured downstream functional pathways such as immune activity and apoptosis. The multi-omics approach with covariate adjustment revealed key radiation-responsive pathways and dose-rate-dependent molecular differences, highlighting the value of integrating transcriptomic and proteomic data to better understand radiation effects.

背景:电离辐射引起复杂的细胞反应,受总剂量和释放速率的影响。了解剂量率如何调节分子结果对准确的风险评估很重要。在这项研究中,我们采用综合多组学方法,结合转录组学和蛋白质组学分析,调整协变量,研究不同剂量率如何改变人类淋巴细胞中的基因和蛋白质表达,重点是关键分子途径的改变。方法:14名健康供体(8名男性,6名女性)的外周血在0-6 Gy的剂量范围内,用0.05 Gy/分钟(DR1)和1.0 Gy/分钟(DR2)的x射线照射体外。采用TempO-Seq™检测基因表达,质谱法检测相对蛋白丰度。采用edgeR和limma进行差异表达分析,调整性别、年龄和白细胞计数(错误发现率(FDR) < 0.05)。使用mixOmics中实现的正则化典型相关分析(rCCA)进行多组学整合,然后进行Reactome途径富集分析。结果:我们分别在DR1和DR2上鉴定了2,477和2,612个差异表达基因,以及368和386个差异表达蛋白。使用来自rCCA的典型变量,我们表明协变量调整改善了剂量辨别,特别是在0.5 Gy以上。使用rCCA的相关截断阈值0.5,鉴定出212对(DR1)和276对(DR2)高度相关的基因蛋白对。DR2暴露与应激相关通路的增强相关,包括未折叠蛋白反应、衰老和致癌激酶信号传导。相反,DR1诱导了与免疫接合相关的途径的富集,包括抗原呈递。在这两种剂量率下,转录组学变化突出了上游调节过程(染色质建模),蛋白质组学变化捕获了下游功能途径,如免疫活性和细胞凋亡。结论:采用协变量调整的多组学方法揭示了关键的辐射反应途径和剂量率依赖的分子差异,突出了整合转录组学和蛋白质组学数据对更好地了解辐射效应的价值。
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引用次数: 0
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Journal of Radiological Protection
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