Pub Date : 2025-01-01Epub Date: 2025-01-25DOI: 10.1038/s44323-024-00020-2
Matt Spick, Cheryl M Isherwood, Lee A Gethings, Christopher J Hughes, Matthew E Daly, Hana Hassanin, Daan R van der Veen, Debra J Skene, Jonathan D Johnston
Time-of-day variation in the molecular profile of biofluids and tissues is a well-described phenomenon, but-especially for proteomics-is rarely considered in terms of the challenges this presents to reproducible biomarker identification. We provide a case study analysis of human circadian and ultradian rhythmicity in proteins, including in the complement and coagulation cascades and apolipoproteins, with PLG, CFAH, ZA2G and ITIH2 demonstrated as rhythmic for the first time. We also show that rhythmicity increases the risk of Type II errors due to the reduction in statistical power from increased variance, and that controlling for rhythmic time-of-day variation improves statistical power and reduces the chances of Type II errors. We recommend that best practice in proteomics study design should account for temporal variation and that time of sampling be reported as part of study metadata. These simple steps can mitigate against both false and missed discoveries, as well as improving reproducibility.
{"title":"Challenges and opportunities for statistical power and biomarker identification arising from rhythmic variation in proteomics.","authors":"Matt Spick, Cheryl M Isherwood, Lee A Gethings, Christopher J Hughes, Matthew E Daly, Hana Hassanin, Daan R van der Veen, Debra J Skene, Jonathan D Johnston","doi":"10.1038/s44323-024-00020-2","DOIUrl":"10.1038/s44323-024-00020-2","url":null,"abstract":"<p><p>Time-of-day variation in the molecular profile of biofluids and tissues is a well-described phenomenon, but-especially for proteomics-is rarely considered in terms of the challenges this presents to reproducible biomarker identification. We provide a case study analysis of human circadian and ultradian rhythmicity in proteins, including in the complement and coagulation cascades and apolipoproteins, with PLG, CFAH, ZA2G and ITIH2 demonstrated as rhythmic for the first time. We also show that rhythmicity increases the risk of Type II errors due to the reduction in statistical power from increased variance, and that controlling for rhythmic time-of-day variation improves statistical power and reduces the chances of Type II errors. We recommend that best practice in proteomics study design should account for temporal variation and that time of sampling be reported as part of study metadata. These simple steps can mitigate against both false and missed discoveries, as well as improving reproducibility.</p>","PeriodicalId":501704,"journal":{"name":"npj Biological Timing and Sleep","volume":"2 1","pages":"3"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-04DOI: 10.1038/s44323-024-00015-z
Anna Katharina Eick, Maite Ogueta, Ralf Stanewsky
Circadian clocks are ubiquitous in almost all organisms on Earth and many key genes are highly conserved among species. In the mammalian suprachiasmatic nucleus, the cAMP response element binding protein (CREB) pathway is known to play a crucial role in conveying light-input to the transcription of clock genes. The fruit fly Drosophila melanogaster also expresses two Creb proteins, CrebA and CrebB, which have been associated with the circadian clock. For example, Drosophila Creb has been suggested to constitute a molecular link between neuronal excitability and clock gene transcription. In this study we subjected flies with clock cell specific CrebA or CrebB mutations to circadian behavioral and bioluminescence assays. Surprisingly, we found that neither loss of CrebA or CrebB did affect free-running locomotor behavior, rhythmic period oscillations in clock neurons, or light-dependent synchronization. In conclusion our findings question the conserved circadian role of the Creb pathway in Drosophila and encourage further studies to elucidate its potential function within insect circadian clocks.
{"title":"Reassessing the involvement of the CREB pathway in the circadian clock of Drosophila melanogaster","authors":"Anna Katharina Eick, Maite Ogueta, Ralf Stanewsky","doi":"10.1038/s44323-024-00015-z","DOIUrl":"10.1038/s44323-024-00015-z","url":null,"abstract":"Circadian clocks are ubiquitous in almost all organisms on Earth and many key genes are highly conserved among species. In the mammalian suprachiasmatic nucleus, the cAMP response element binding protein (CREB) pathway is known to play a crucial role in conveying light-input to the transcription of clock genes. The fruit fly Drosophila melanogaster also expresses two Creb proteins, CrebA and CrebB, which have been associated with the circadian clock. For example, Drosophila Creb has been suggested to constitute a molecular link between neuronal excitability and clock gene transcription. In this study we subjected flies with clock cell specific CrebA or CrebB mutations to circadian behavioral and bioluminescence assays. Surprisingly, we found that neither loss of CrebA or CrebB did affect free-running locomotor behavior, rhythmic period oscillations in clock neurons, or light-dependent synchronization. In conclusion our findings question the conserved circadian role of the Creb pathway in Drosophila and encourage further studies to elucidate its potential function within insect circadian clocks.","PeriodicalId":501704,"journal":{"name":"npj Biological Timing and Sleep","volume":" ","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44323-024-00015-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142762903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-04DOI: 10.1038/s44323-024-00016-y
Danielle A. Wallace
Light is the primary entraining cue for the circadian system and has other, non-circadian, effects on health. Sex differences in light exposure patterns could drive sex differences in health outcomes. Real-world light exposure (measured with wrist-worn ActiGraph GT3X+) was investigated in the 2011–2014 U.S.-based National Health and Nutrition Examination Survey (NHANES) with metrics reflecting duration in and timing of exposure at different light levels. Sex differences were tested using two-sample t-tests and linear regression models. Occupational and physical activity-related factors were also explored as contributing factors using linear regression models adjusted for age, season, and race/ethnicity. 11,314 NHANES participants (age range: 3–80+, 52.2% females) were included in the analysis. The results show males spending approximately 52% more time in bright light than females, with this sex difference beginning in childhood. While further research is needed, these findings may be due to sex differences in indoor vs. outdoor activities.
{"title":"Light exposure differs by sex in the US, with females receiving less bright light","authors":"Danielle A. Wallace","doi":"10.1038/s44323-024-00016-y","DOIUrl":"10.1038/s44323-024-00016-y","url":null,"abstract":"Light is the primary entraining cue for the circadian system and has other, non-circadian, effects on health. Sex differences in light exposure patterns could drive sex differences in health outcomes. Real-world light exposure (measured with wrist-worn ActiGraph GT3X+) was investigated in the 2011–2014 U.S.-based National Health and Nutrition Examination Survey (NHANES) with metrics reflecting duration in and timing of exposure at different light levels. Sex differences were tested using two-sample t-tests and linear regression models. Occupational and physical activity-related factors were also explored as contributing factors using linear regression models adjusted for age, season, and race/ethnicity. 11,314 NHANES participants (age range: 3–80+, 52.2% females) were included in the analysis. The results show males spending approximately 52% more time in bright light than females, with this sex difference beginning in childhood. While further research is needed, these findings may be due to sex differences in indoor vs. outdoor activities.","PeriodicalId":501704,"journal":{"name":"npj Biological Timing and Sleep","volume":" ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44323-024-00016-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142762913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1038/s44323-024-00012-2
Carlos C. Flores, Nickolas A. Pasetto, Hongyang Wang, Alexander G. Dimitrov, Jon F. Davis, Zhihua Jiang, Christopher J. Davis, Jason R. Gerstner
Disruption of sleep and circadian rhythms are a comorbid feature of many pathologies, and can negatively influence many health conditions, including neurodegenerative disease, metabolic illness, cancer, and various neurological disorders. Genetic association studies linking sleep and circadian disturbances with disease susceptibility have mainly focused on changes in gene expression due to mutations, such as single-nucleotide polymorphisms. The interaction between sleep and/or circadian rhythms with the use of Alternative Polyadenylation (APA) has been largely undescribed, particularly in the context of other disorders. APA generates transcript isoforms by utilizing various polyadenylation sites (PASs) from the same gene affecting its mRNA translation, stability, localization, and subsequent function. Here we identified unique APAs expressed in rat brain over time-of-day, immediately following sleep deprivation, and the subsequent recovery period. From these data, we performed a secondary analysis of these sleep- or time-of-day associated PASs with recently described APA-linked human brain disorder susceptibility genes.
睡眠和昼夜节律紊乱是许多疾病的并发症，会对许多健康状况产生负面影响，包括神经退行性疾病、代谢性疾病、癌症和各种神经系统疾病。将睡眠和昼夜节律紊乱与疾病易感性联系起来的遗传关联研究主要集中于基因突变（如单核苷酸多态性）导致的基因表达变化。睡眠和/或昼夜节律与交替多腺苷酸化（APA）之间的相互作用在很大程度上尚未被描述，尤其是在其他疾病的背景下。APA 通过利用同一基因的不同多腺苷酸化位点（PAS）产生转录本异构体，从而影响其 mRNA 的翻译、稳定性、定位和后续功能。在这里，我们发现了大鼠大脑在不同时间段、睡眠剥夺后和随后的恢复期所表达的独特 APA。根据这些数据，我们对这些与睡眠或睡眠时间相关的 PAS 与最近描述的与 APA 相关的人类脑部疾病易感基因进行了二次分析。
{"title":"Sleep and diurnal alternative polyadenylation sites associated with human APA-linked brain disorders","authors":"Carlos C. Flores, Nickolas A. Pasetto, Hongyang Wang, Alexander G. Dimitrov, Jon F. Davis, Zhihua Jiang, Christopher J. Davis, Jason R. Gerstner","doi":"10.1038/s44323-024-00012-2","DOIUrl":"10.1038/s44323-024-00012-2","url":null,"abstract":"Disruption of sleep and circadian rhythms are a comorbid feature of many pathologies, and can negatively influence many health conditions, including neurodegenerative disease, metabolic illness, cancer, and various neurological disorders. Genetic association studies linking sleep and circadian disturbances with disease susceptibility have mainly focused on changes in gene expression due to mutations, such as single-nucleotide polymorphisms. The interaction between sleep and/or circadian rhythms with the use of Alternative Polyadenylation (APA) has been largely undescribed, particularly in the context of other disorders. APA generates transcript isoforms by utilizing various polyadenylation sites (PASs) from the same gene affecting its mRNA translation, stability, localization, and subsequent function. Here we identified unique APAs expressed in rat brain over time-of-day, immediately following sleep deprivation, and the subsequent recovery period. From these data, we performed a secondary analysis of these sleep- or time-of-day associated PASs with recently described APA-linked human brain disorder susceptibility genes.","PeriodicalId":501704,"journal":{"name":"npj Biological Timing and Sleep","volume":" ","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44323-024-00012-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1038/s44323-024-00011-3
Malena Mul Fedele, Leandro P. Casiraghi, Santiago A. Plano, Giannina Bellone, Diego A. Golombek, Daniel E. Vigo
We compared three different work schedules in a large oil company: 1) two days of 12 h of daytime shifts followed by two consecutive 12 h night shifts, followed by four work-free days (“2 x 2 x 4”), 2) four consecutive 12 h daytime shifts and four consecutive 12 h night shifts, flanked by four work-free days (“4 x 4 x 4”), 3) a non-rotating schedule involving continuous 12 h daytime shifts during 40 days (“fixed 12 h”). We measured wrist-actigraphy, peripheral temperature rhythms, and subjective self-reports regarding fatigue, somnolence, and psycho-affective features. Sleep duration on the resting period was significantly less than the recommended 7 h. The “2 x 2 x 4” schedule resulted in decreased sleep regularity and increased circadian disruption, higher levels of insomnia, increased fatigue impact, lower alertness levels, and heightened symptoms of depression associated with more nocturnal sleep time after diurnal work. Our findings indicate that health and safety vary depending on the type of schedule employed.
我们比较了一家大型石油公司的三种不同工作安排：1）两天 12 小时的白班，然后是连续两次 12 小时的夜班，接着是四天的无工作日（"2 x 2 x 4"）；2）连续四次 12 小时的白班和连续四次 12 小时的夜班，中间是四天的无工作日（"4 x 4 x 4"）；3）40 天内连续 12 小时白班的非轮换时间表（"固定 12 小时"）。我们测量了腕动仪、外周温度节律以及有关疲劳、嗜睡和心理情感特征的主观自我报告。2 x 2 x 4 "时间表导致睡眠规律性降低、昼夜节律紊乱加剧、失眠程度升高、疲劳影响增加、警觉性降低，以及与昼间工作后夜间睡眠时间增加有关的抑郁症状加剧。我们的研究结果表明，健康和安全因采用的时间表类型而异。
{"title":"Bridging the gap: examining circadian biology and fatigue alongside work schedules","authors":"Malena Mul Fedele, Leandro P. Casiraghi, Santiago A. Plano, Giannina Bellone, Diego A. Golombek, Daniel E. Vigo","doi":"10.1038/s44323-024-00011-3","DOIUrl":"10.1038/s44323-024-00011-3","url":null,"abstract":"We compared three different work schedules in a large oil company: 1) two days of 12 h of daytime shifts followed by two consecutive 12 h night shifts, followed by four work-free days (“2 x 2 x 4”), 2) four consecutive 12 h daytime shifts and four consecutive 12 h night shifts, flanked by four work-free days (“4 x 4 x 4”), 3) a non-rotating schedule involving continuous 12 h daytime shifts during 40 days (“fixed 12 h”). We measured wrist-actigraphy, peripheral temperature rhythms, and subjective self-reports regarding fatigue, somnolence, and psycho-affective features. Sleep duration on the resting period was significantly less than the recommended 7 h. The “2 x 2 x 4” schedule resulted in decreased sleep regularity and increased circadian disruption, higher levels of insomnia, increased fatigue impact, lower alertness levels, and heightened symptoms of depression associated with more nocturnal sleep time after diurnal work. Our findings indicate that health and safety vary depending on the type of schedule employed.","PeriodicalId":501704,"journal":{"name":"npj Biological Timing and Sleep","volume":" ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44323-024-00011-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1038/s44323-024-00014-0
Viviane Akemi Kakazu, Marcia Assis, Andrea Bacelar, Andréia Gomes Bezerra, Giovanna Lira Rosa Ciutti, Silvia Gonçalves Conway, José Carlos Fernandes Galduróz, Luciano F. Drager, Mariana Pery Khoury, Ingrid Porto Araújo Leite, Ygor de Matos Luciano, Dalva Poyares, Sergio Tufik, Gabriel Natan Pires
This study aimed to describe the publication profile of randomized controlled trials (RCTs) for insomnia. A systematic review of RCTs regarding interventions for non-comorbid insomnia in adults retrieved 132 RCTs: 58 related to pharmacological treatments, 71 to non-pharmacological treatments, and 3 to both interventions. The treatments with the biggest publication profile were digital CBT-I (dCBT-I) (n = 35), in-person CBT-I (n = 28) and zolpidem (n = 22). Regarding dCBT-I, the median publication year is 2019, with 1.13 ± 1.91 RCTs published per year. Regarding zolpidem, the median publication year is 2008, with 0.71 ± 0.97 RCTs per year. Regarding in-person CBT-I, the median publication year is 2018, with 0.90 ± 1.14 RCTs per year. The majority of the available RCTs are on non-pharmacological interventions, particularly CBT-I (mostly in the 2000s) and dCBT-I (mostly in the last decade), although presenting a reduced methodological quality in comparison to pharmacological interventions. These data suggest an increasing focus on non-pharmacological interventions for insomnia.
{"title":"Insomnia and its treatments—trend analysis and publication profile of randomized clinical trials","authors":"Viviane Akemi Kakazu, Marcia Assis, Andrea Bacelar, Andréia Gomes Bezerra, Giovanna Lira Rosa Ciutti, Silvia Gonçalves Conway, José Carlos Fernandes Galduróz, Luciano F. Drager, Mariana Pery Khoury, Ingrid Porto Araújo Leite, Ygor de Matos Luciano, Dalva Poyares, Sergio Tufik, Gabriel Natan Pires","doi":"10.1038/s44323-024-00014-0","DOIUrl":"10.1038/s44323-024-00014-0","url":null,"abstract":"This study aimed to describe the publication profile of randomized controlled trials (RCTs) for insomnia. A systematic review of RCTs regarding interventions for non-comorbid insomnia in adults retrieved 132 RCTs: 58 related to pharmacological treatments, 71 to non-pharmacological treatments, and 3 to both interventions. The treatments with the biggest publication profile were digital CBT-I (dCBT-I) (n = 35), in-person CBT-I (n = 28) and zolpidem (n = 22). Regarding dCBT-I, the median publication year is 2019, with 1.13 ± 1.91 RCTs published per year. Regarding zolpidem, the median publication year is 2008, with 0.71 ± 0.97 RCTs per year. Regarding in-person CBT-I, the median publication year is 2018, with 0.90 ± 1.14 RCTs per year. The majority of the available RCTs are on non-pharmacological interventions, particularly CBT-I (mostly in the 2000s) and dCBT-I (mostly in the last decade), although presenting a reduced methodological quality in comparison to pharmacological interventions. These data suggest an increasing focus on non-pharmacological interventions for insomnia.","PeriodicalId":501704,"journal":{"name":"npj Biological Timing and Sleep","volume":" ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44323-024-00014-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1038/s44323-024-00013-1
Pureum Kim, Nicholas Garner, Annaleis Tatkovic, Rex Parsons, Prasad Chunduri, Jana Vukovic, Michael Piper, Martina Pfeffer, Marco Weiergräber, Henrik Oster, Oliver Rawashdeh
Melatonin supplementation strengthens non‐restorative sleep rhythms and its temporal alignment in both humans and night-active rodents. Of note, although the sleep cycle is reversed in day-active and night-active (nocturnal) mammals, both, produce melatonin at night under the control of the circadian clock. The effects of exogenous melatonin on sleep and sleepiness are relatively clear, but its endogenous role in sleep, particularly, in timing sleep onset (SO), remains poorly understood. We show in nocturnal mice that the increases in mid-nighttime sleep episodes, and the mid-nighttime decline in activity, are coupled to nighttime melatonin signaling. Furthermore, we show that endogenous melatonin modulates SO by reducing the threshold for wake-to-sleep transitioning. Such link between melatonin and SO timing may explain phenomena such as increased sleep propensity in circadian rhythm sleep disorders and chronic insomnia in patients with severely reduced nocturnal melatonin levels. Our findings demonstrate that melatonin’s role in sleep is evolutionarily conserved, effectively challenging the argument that melatonin cannot play a major role in sleep regulation in nocturnal mammals, where the main activity phase coincides with high melatonin levels.
{"title":"Melatonin’s role in the timing of sleep onset is conserved in nocturnal mice","authors":"Pureum Kim, Nicholas Garner, Annaleis Tatkovic, Rex Parsons, Prasad Chunduri, Jana Vukovic, Michael Piper, Martina Pfeffer, Marco Weiergräber, Henrik Oster, Oliver Rawashdeh","doi":"10.1038/s44323-024-00013-1","DOIUrl":"10.1038/s44323-024-00013-1","url":null,"abstract":"Melatonin supplementation strengthens non‐restorative sleep rhythms and its temporal alignment in both humans and night-active rodents. Of note, although the sleep cycle is reversed in day-active and night-active (nocturnal) mammals, both, produce melatonin at night under the control of the circadian clock. The effects of exogenous melatonin on sleep and sleepiness are relatively clear, but its endogenous role in sleep, particularly, in timing sleep onset (SO), remains poorly understood. We show in nocturnal mice that the increases in mid-nighttime sleep episodes, and the mid-nighttime decline in activity, are coupled to nighttime melatonin signaling. Furthermore, we show that endogenous melatonin modulates SO by reducing the threshold for wake-to-sleep transitioning. Such link between melatonin and SO timing may explain phenomena such as increased sleep propensity in circadian rhythm sleep disorders and chronic insomnia in patients with severely reduced nocturnal melatonin levels. Our findings demonstrate that melatonin’s role in sleep is evolutionarily conserved, effectively challenging the argument that melatonin cannot play a major role in sleep regulation in nocturnal mammals, where the main activity phase coincides with high melatonin levels.","PeriodicalId":501704,"journal":{"name":"npj Biological Timing and Sleep","volume":" ","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44323-024-00013-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01DOI: 10.1038/s44323-024-00010-4
Manuel Spitschan, Laura Kervezee, Renske Lok, Elise McGlashan, Raymond P. Najjar
The transition from postdoc to junior faculty is exciting and uniquely challenging. On one hand, it allows for increased creative freedom and the opportunity to grow into an independent scientist. On the other hand, it comes with increasing administrative responsibilities, feelings of isolation, and high pressure to perform. The result is an environment that can leave very limited time for creative thinking and reflection. Here, we describe how participating in a program that allowed us to step out of our routine and work together helped us become more independent—and regain time to think.
{"title":"How stepping out helped us tune in: finding space and time to think as an early career researcher","authors":"Manuel Spitschan, Laura Kervezee, Renske Lok, Elise McGlashan, Raymond P. Najjar","doi":"10.1038/s44323-024-00010-4","DOIUrl":"10.1038/s44323-024-00010-4","url":null,"abstract":"The transition from postdoc to junior faculty is exciting and uniquely challenging. On one hand, it allows for increased creative freedom and the opportunity to grow into an independent scientist. On the other hand, it comes with increasing administrative responsibilities, feelings of isolation, and high pressure to perform. The result is an environment that can leave very limited time for creative thinking and reflection. Here, we describe how participating in a program that allowed us to step out of our routine and work together helped us become more independent—and regain time to think.","PeriodicalId":501704,"journal":{"name":"npj Biological Timing and Sleep","volume":" ","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44323-024-00010-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142360086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01DOI: 10.1038/s44323-024-00009-x
Bryan R. Alava, Andrew R. Morris, Andrew C. Liu, Jose F. Abisambra, Karyn A. Esser
Sleep timing and quantity disturbances persist in tauopathy patients. This has been studied in transgenic models of primary tau neuropathology using traditional electroencephalograms (EEGs) and more recently, the PiezoSleep Mouse Behavioral Tracking System. Here, we generated a primary tauopathy model using an intracerebroventricular injection of human mutant hSyn-P301L-tau, using adeno-associated virus of serotype 8 (AAV8). We discovered distinctions in sleep architecture with altered quantity and timing in AAV8-P301L tau expressing mice of both sexes using the noninvasive PiezoSleep System. The AAV8-P301L tau mice exhibit striking age-related increases in sleep duration specifically at the active phase onset, suggesting a critical and sensitive time-of-day for tauopathy related sleep disturbances to occur. Since our findings show sleep behavior changes at specific transitional periods of the day, tau neuropathology may impact normal diurnal variation in biological processes, which should be explored using the AAV8-P301L tauopathy model.
tau蛋白病患者的睡眠时间和数量障碍持续存在。利用传统脑电图（EEG）和最近的压电睡眠小鼠行为跟踪系统（PiezoSleep Mouse Behavioral Tracking System）在原发性tau神经病理的转基因模型中对此进行了研究。在这里，我们利用 8 号血清型腺相关病毒（AAV8），通过脑室内注射人类突变体 hSyn-P301L-tau，建立了原发性 tau 病模型。我们利用无创压电睡眠系统（PiezoSleep System）发现，在表达 AAV8-P301L tau 的雌雄小鼠中，睡眠结构的数量和时间都发生了改变。AAV8-P301L tau小鼠的睡眠持续时间与年龄相关，特别是在活跃期开始时显著增加，这表明与tauopathy相关的睡眠障碍发生在一天中的一个关键而敏感的时间段。由于我们的研究结果表明睡眠行为在一天中特定的过渡时期会发生变化，因此tau神经病变可能会影响生物过程的正常昼夜变化，这一点应使用AAV8-P301L tau病模型进行探索。
{"title":"AAV8-P301L tau expression confers age-related disruptions in sleep quantity and timing","authors":"Bryan R. Alava, Andrew R. Morris, Andrew C. Liu, Jose F. Abisambra, Karyn A. Esser","doi":"10.1038/s44323-024-00009-x","DOIUrl":"10.1038/s44323-024-00009-x","url":null,"abstract":"Sleep timing and quantity disturbances persist in tauopathy patients. This has been studied in transgenic models of primary tau neuropathology using traditional electroencephalograms (EEGs) and more recently, the PiezoSleep Mouse Behavioral Tracking System. Here, we generated a primary tauopathy model using an intracerebroventricular injection of human mutant hSyn-P301L-tau, using adeno-associated virus of serotype 8 (AAV8). We discovered distinctions in sleep architecture with altered quantity and timing in AAV8-P301L tau expressing mice of both sexes using the noninvasive PiezoSleep System. The AAV8-P301L tau mice exhibit striking age-related increases in sleep duration specifically at the active phase onset, suggesting a critical and sensitive time-of-day for tauopathy related sleep disturbances to occur. Since our findings show sleep behavior changes at specific transitional periods of the day, tau neuropathology may impact normal diurnal variation in biological processes, which should be explored using the AAV8-P301L tauopathy model.","PeriodicalId":501704,"journal":{"name":"npj Biological Timing and Sleep","volume":" ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44323-024-00009-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142360083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01DOI: 10.1038/s44323-024-00008-y
Emerson M. Wickwire, Jacob Collen, Vincent F. Capaldi II, Zhiwei Zhao, Scott G. Williams, Connie L. Thomas, Samson Z. Assefa, Jennifer S. Albrecht, Shuo Chen
This study employed remote monitoring/ecological momentary assessment methods to test the hypothesis that prior-night sleep is associated with next-day symptoms. Military personnel with sleep problems (N = 270) completed daily sleep diaries and twice-daily symptom surveys via smartphone and wore a commercial wearable for ten days. In lagged analyses controlling for age and sex, prior-night sleep was robustly associated with next-day symptoms. Findings support remote approaches to assess sleep and next-day symptoms.
{"title":"Prior-night sleep predicts next-day symptoms over ten days among military personnel with sleep problems","authors":"Emerson M. Wickwire, Jacob Collen, Vincent F. Capaldi II, Zhiwei Zhao, Scott G. Williams, Connie L. Thomas, Samson Z. Assefa, Jennifer S. Albrecht, Shuo Chen","doi":"10.1038/s44323-024-00008-y","DOIUrl":"10.1038/s44323-024-00008-y","url":null,"abstract":"This study employed remote monitoring/ecological momentary assessment methods to test the hypothesis that prior-night sleep is associated with next-day symptoms. Military personnel with sleep problems (N = 270) completed daily sleep diaries and twice-daily symptom surveys via smartphone and wore a commercial wearable for ten days. In lagged analyses controlling for age and sex, prior-night sleep was robustly associated with next-day symptoms. Findings support remote approaches to assess sleep and next-day symptoms.","PeriodicalId":501704,"journal":{"name":"npj Biological Timing and Sleep","volume":" ","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44323-024-00008-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142360084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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