The need for rapid and cheap synthesis of large numbers of chemical compounds has contributed to the emergence of combinatorial chemistry (simultaneous synthesis of different compounds, in contrast to traditional synthesis, in which each substance is produced individually). Combinatorial library methods were initially applied only to peptides and oligonucleotides. By now, the scope of these libraries has expanded considerably to include proteins, synthetic oligomers, small molecules, and oligosaccharides. The enormous variety of antibodies (Abs) makes it possible to detect clones able to interact highly specifically with almost any natural or synthetic antigen (Ag). Phage Abs are an excellent alternative to mono-and polyclonal Abs, because they are highly stable, have no disulfide bonds, and are much cheaper to make. Monitoring of various substances, including proteins, in a living organism is much in demand. Despite the vast amount of literature available on Ab phage display, the use of phage display to determine diagnostically important Ags has not been sufficiently covered. Many studies have confirmed that unlike other types of Abs, phage Abs ensure highly sensitive Ag detection. Therefore, this review focuses on the use of phage display to prepare Abs specific to diagnostically important Ags (allergens, disease and cancer biomarkers, toxins) and on their application in analytical systems, including biosensors. The use of phage Abs in Ag diagnostics is compared with the use of classical Abs, and the prospects are shown for the use of phage Abs as biosensor sensing elements. This review analyzes the recent advances in the detection of diagnostically important Ags by using phage display–based biosensors. Systematic information is presented about allergens, disease and cancer biomarkers, and toxins detected by using phage Abs. Phage display Abs for sensor-based Ag detection are presented as an affordable alternative to classic tests.
对快速、廉价合成大量化合物的需求促进了组合化学的出现（同时合成不同的化合物，而不是传统合成法中的每种物质单独生产）。组合库方法最初只应用于肽和寡核苷酸。现在，这些文库的范围已大大扩展，包括蛋白质、合成寡聚体、小分子和寡糖。由于抗体（Abs）种类繁多，因此可以检测到能与几乎所有天然或合成抗原（Ag）发生高度特异性相互作用的克隆。噬菌体抗体是单克隆和多克隆抗体的最佳替代品，因为它们高度稳定，没有二硫键，而且制造成本更低。对生物体内各种物质（包括蛋白质）的监测需求量很大。尽管关于抗体噬菌体展示的文献数量庞大，但利用噬菌体展示来确定诊断上重要的抗体的研究还不够深入。许多研究证实，与其他类型的抗体不同，噬菌体抗体可确保高灵敏度的抗体检测。因此，本综述将重点介绍如何利用噬菌体展示技术制备特异性Abs，用于诊断重要的Ags（过敏原、疾病和癌症生物标记物、毒素），以及它们在分析系统（包括生物传感器）中的应用。噬菌体吸附剂在 Ag 诊断中的应用与传统吸附剂的应用进行了比较，并展示了将噬菌体吸附剂用作生物传感器传感元件的前景。这篇综述分析了利用基于噬菌体展示的生物传感器检测具有诊断意义的 Ags 的最新进展。系统介绍了利用噬菌体Abs检测过敏原、疾病和癌症生物标记物以及毒素的相关信息。噬菌体展示Abs用于基于传感器的Ag检测，是传统检测方法的一种经济实惠的替代方法。
{"title":"Phage Antibodies for Detection of Diagnostically Important Antigens","authors":"OlgaI. Guliy, V. A. Khanadeev, L. Dykman","doi":"10.31083/j.fbe1603024","DOIUrl":"https://doi.org/10.31083/j.fbe1603024","url":null,"abstract":"The need for rapid and cheap synthesis of large numbers of chemical compounds has contributed to the emergence of combinatorial chemistry (simultaneous synthesis of different compounds, in contrast to traditional synthesis, in which each substance is produced individually). Combinatorial library methods were initially applied only to peptides and oligonucleotides. By now, the scope of these libraries has expanded considerably to include proteins, synthetic oligomers, small molecules, and oligosaccharides. The enormous variety of antibodies (Abs) makes it possible to detect clones able to interact highly specifically with almost any natural or synthetic antigen (Ag). Phage Abs are an excellent alternative to mono-and polyclonal Abs, because they are highly stable, have no disulfide bonds, and are much cheaper to make. Monitoring of various substances, including proteins, in a living organism is much in demand. Despite the vast amount of literature available on Ab phage display, the use of phage display to determine diagnostically important Ags has not been sufficiently covered. Many studies have confirmed that unlike other types of Abs, phage Abs ensure highly sensitive Ag detection. Therefore, this review focuses on the use of phage display to prepare Abs specific to diagnostically important Ags (allergens, disease and cancer biomarkers, toxins) and on their application in analytical systems, including biosensors. The use of phage Abs in Ag diagnostics is compared with the use of classical Abs, and the prospects are shown for the use of phage Abs as biosensor sensing elements. This review analyzes the recent advances in the detection of diagnostically important Ags by using phage display–based biosensors. Systematic information is presented about allergens, disease and cancer biomarkers, and toxins detected by using phage Abs. Phage display Abs for sensor-based Ag detection are presented as an affordable alternative to classic tests.","PeriodicalId":502751,"journal":{"name":"Frontiers in Bioscience-Elite","volume":"96 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141657944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background : Rhizobial inoculation in combination with fungicidal seed treatment is an effective solution for improving soybean resistance to modern climate changes due to the maximum implementation of the plant’s stress-protective antioxidant properties and their nitrogen-fixing potential, which will contribute to the preservation of the environment. Methods : Model ecosystems at different stages of legume–rhizobial symbiosis formation, created by treatment before sowing soybean seeds with a fungicide (fludioxonil, 25 g/L) and inoculation with an active strain of Bradyrhizobium japonicum (titer 10 9 cells per mL), were subjected to microbiological, biochemical, and physiological testing methods in controlled and field conditions. Results : Seed treatment with fungicide and rhizobia showed different patterns in the dynamics of key antioxidant enzymes in soybean nodules under drought conditions. Superoxide dismutase activity increased by 32.7% under moderate stress, while catalase increased by 90.6% under long-term stress. An increase in the antioxidant enzyme activity induced the regulation of lipoperoxidation processes during drought and after the restoration of irrigation. Regeneration after stress was evident in soybean plants with a combination of fungicide seed treatment and rhizobial inoculant, where enzyme levels and lipoperoxidation processes returned to control plant levels. Applying seed treatment with fungicide and Rhizobium led to the preservation of the symbiotic apparatus functioning in drought conditions. As proof of this, molecular nitrogen fixation by nodules has a higher efficiency of 25.6% compared to soybeans without fungicide treatment. In the field, fungicidal treatment of seeds in a complex with rhizobia inoculant induced prolongation of the symbiotic apparatus functioning in the reproductive period of soybean ontogenesis. This positively affected the nitrogen-fixing activity of soybeans during the pod formation stage by more than 71.7%, as well as increasing soybean yield by 12.7% in the field. Conclusions : The application of Rhizobium inoculant and fungicide to seeds contributed to the development of antioxidant protection of soybean plants during droughts due to the activation of key enzymatic complexes and regulation of lipoperoxidation processes, which have a positive effect on nitrogen fixation and productivity of soybeans. This is a necessary element in soybean agrotechnologies to improve plant adaptation and resilience in the context of modern climate change.
{"title":"Rhizobium Inoculant and Seed-Applied Fungicide Effects Improve the Drought Tolerance of Soybean Plants as an Effective Agroecological Solution under Climate Change Conditions","authors":"T. Nyzhnyk, S. Kots, P. Pukhtaievych","doi":"10.31083/j.fbe1603023","DOIUrl":"https://doi.org/10.31083/j.fbe1603023","url":null,"abstract":"Background : Rhizobial inoculation in combination with fungicidal seed treatment is an effective solution for improving soybean resistance to modern climate changes due to the maximum implementation of the plant’s stress-protective antioxidant properties and their nitrogen-fixing potential, which will contribute to the preservation of the environment. Methods : Model ecosystems at different stages of legume–rhizobial symbiosis formation, created by treatment before sowing soybean seeds with a fungicide (fludioxonil, 25 g/L) and inoculation with an active strain of Bradyrhizobium japonicum (titer 10 9 cells per mL), were subjected to microbiological, biochemical, and physiological testing methods in controlled and field conditions. Results : Seed treatment with fungicide and rhizobia showed different patterns in the dynamics of key antioxidant enzymes in soybean nodules under drought conditions. Superoxide dismutase activity increased by 32.7% under moderate stress, while catalase increased by 90.6% under long-term stress. An increase in the antioxidant enzyme activity induced the regulation of lipoperoxidation processes during drought and after the restoration of irrigation. Regeneration after stress was evident in soybean plants with a combination of fungicide seed treatment and rhizobial inoculant, where enzyme levels and lipoperoxidation processes returned to control plant levels. Applying seed treatment with fungicide and Rhizobium led to the preservation of the symbiotic apparatus functioning in drought conditions. As proof of this, molecular nitrogen fixation by nodules has a higher efficiency of 25.6% compared to soybeans without fungicide treatment. In the field, fungicidal treatment of seeds in a complex with rhizobia inoculant induced prolongation of the symbiotic apparatus functioning in the reproductive period of soybean ontogenesis. This positively affected the nitrogen-fixing activity of soybeans during the pod formation stage by more than 71.7%, as well as increasing soybean yield by 12.7% in the field. Conclusions : The application of Rhizobium inoculant and fungicide to seeds contributed to the development of antioxidant protection of soybean plants during droughts due to the activation of key enzymatic complexes and regulation of lipoperoxidation processes, which have a positive effect on nitrogen fixation and productivity of soybeans. This is a necessary element in soybean agrotechnologies to improve plant adaptation and resilience in the context of modern climate change.","PeriodicalId":502751,"journal":{"name":"Frontiers in Bioscience-Elite","volume":"2 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141661584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background : Chicken feathers contribute to large quantities of keratinaceous wastes that pose serious environmental problems and must be catered to properly. Chicken feathers are also a potential source of vital proteins, peptides
{"title":"Characterization of a Bacterial Keratinolytic Protease for Effective Degradation of Chicken Feather Waste into Feather Protein Hydrolysates","authors":"Armish Riaz, Hira Muzzamal, Beenish Maqsood, Shumaila Naz, Farooq Latif, Mahjabeen Saleem","doi":"10.31083/j.fbe1603022","DOIUrl":"https://doi.org/10.31083/j.fbe1603022","url":null,"abstract":"Background : Chicken feathers contribute to large quantities of keratinaceous wastes that pose serious environmental problems and must be catered to properly. Chicken feathers are also a potential source of vital proteins, peptides","PeriodicalId":502751,"journal":{"name":"Frontiers in Bioscience-Elite","volume":"121 51","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141666202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Lauková, L. Chrastinová, I. Plachá, V. Focková, Natália Zábolyová, Eva Bino, Ľ. Grešáková, Rudolf Žitňan, Z. Formelová, J. Ščerbová, G. Bełżecki, R. Miltko, M. Pogány Simonová
Background : Using bacterial (probiotic) strains can influence beneficial health statuses, e
背景：使用细菌（益生菌）菌株可以影响有益的健康状况，例如
{"title":"Dairy-Derived and Bacteriocin-Producing Strain Lactiplantibacillus plantarum LP17L/1: An Assessment of Its Safety and Effect Using Broiler Rabbits","authors":"A. Lauková, L. Chrastinová, I. Plachá, V. Focková, Natália Zábolyová, Eva Bino, Ľ. Grešáková, Rudolf Žitňan, Z. Formelová, J. Ščerbová, G. Bełżecki, R. Miltko, M. Pogány Simonová","doi":"10.31083/j.fbe1603021","DOIUrl":"https://doi.org/10.31083/j.fbe1603021","url":null,"abstract":"Background : Using bacterial (probiotic) strains can influence beneficial health statuses, e","PeriodicalId":502751,"journal":{"name":"Frontiers in Bioscience-Elite","volume":" 23","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141675272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diyorbek Kosimov, R. Ergashev, Aziza Mavjudova, N. Lazutin, Tatyana Khegay, Lyudmila Zaynitdinova
{"title":"Isolation, Characteristics, and Prospects of Using the Ochrobactrum Intermedium Strain in the Degradation of the Cypermethrin Pesticide","authors":"Diyorbek Kosimov, R. Ergashev, Aziza Mavjudova, N. Lazutin, Tatyana Khegay, Lyudmila Zaynitdinova","doi":"10.31083/j.fbe1602020","DOIUrl":"https://doi.org/10.31083/j.fbe1602020","url":null,"abstract":"","PeriodicalId":502751,"journal":{"name":"Frontiers in Bioscience-Elite","volume":"44 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141338723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Komagataella phaffii (K. phaffii) ( Pichia pastoris ), also called biotech yeast, is a yeast species with many applications in the biotechnology and pharmaceutical industries. This methylotrophic yeast has garnered significant interest as a platform for the production of recombinant proteins. Numerous benefits include effective secretory expression that facilitates the easy purification of heterologous proteins, high cell density with rapid growth, post-translational changes, and stable gene expression with integration into the genome. In the last thirty years, K. phaffii has also been refined as an adaptable cell factory that can produce hundreds of biomolecules in a laboratory setting and on an industrial scale. Indeed, over 5000 recombinant proteins have been generated so far using the K. phaffii expression method, which makes up 30% of the total cell protein or 80% of the total released protein. K. phaffii has been used to manufacture more than 70 commercial products in addition to over 300 industrial processes that have been granted licenses. Among these are useful enzymes for industrial biotechnology, including xylanase, mannanase, lipase, and phytase. The others are biopharmaceuticals, which include human serum albumin, insulin, hepatitis B surface antigen, and epidermal growth factor. Compared to other expression systems, this yeast is also considered a special host for synthesizing subunit vaccines, which have recently been supplanted by alternative vaccination types, such as inactivated/killed and live attenuated vaccines. Moreover, efficient production of recombinant proteins is achieved through multi-level optimization methods, such as codon bias, gene dosage, promoters, signal peptides, and environmental factors. Therefore, although K. phaffii expression systems are efficient and simple with clearly established process procedures, it is still necessary to determine the ideal conditions since these vary depending on the target protein to ensure the highest recombinant protein generation. This review addresses the K. phaffii expression system, its importance in industrial and biopharmaceutical protein production, and some bioprocessing and genetic modification strategies for efficient protein production. K. phaffii will eventually continue contributing as a potent expression system in research areas and industrial applications.
Komagataella phaffii（K. phaffii）（Pichia pastoris），又称生物技术酵母，是一种在生物技术和制药业中应用广泛的酵母菌。作为生产重组蛋白质的平台，这种甲基营养酵母引起了人们的极大兴趣。这种酵母具有许多优点，包括有效的分泌表达，便于纯化异源蛋白；细胞密度高，生长迅速；翻译后变化；基因表达稳定，可整合到基因组中。在过去的 30 年中，K. phaffii 还被改良为一种适应性强的细胞工厂，可在实验室环境和工业规模下生产数百种生物分子。事实上，迄今为止，利用 K. phaffii 的表达方法已经产生了 5000 多种重组蛋白，占细胞蛋白总量的 30% 或释放蛋白总量的 80%。K. phaffii 已被用于生产 70 多种商业产品，此外还有 300 多种工业工艺已获得许可。其中有用的酶用于工业生物技术，包括木聚糖酶、甘露聚糖酶、脂肪酶和植酸酶。其他是生物制药，包括人血清白蛋白、胰岛素、乙型肝炎表面抗原和表皮生长因子。与其他表达系统相比，这种酵母还被认为是合成亚单位疫苗的特殊宿主，亚单位疫苗近来已被灭活/杀伤疫苗和减毒活疫苗等其他疫苗类型所取代。此外，重组蛋白的高效生产是通过多层次优化方法实现的，如密码子偏差、基因剂量、启动子、信号肽和环境因素。因此，尽管 K. phaffii 表达系统高效、简单，有明确的工艺程序，但仍有必要确定理想的条件，因为这些条件因目标蛋白而异，以确保产生最高的重组蛋白。本综述论述了 K. phaffii 表达系统、其在工业和生物制药蛋白质生产中的重要性，以及一些高效蛋白质生产的生物处理和基因修饰策略。K. phaffii最终将继续作为一种有效的表达系统在研究领域和工业应用中作出贡献。
{"title":"Komagataella phaffii (Pichia pastoris) as a Powerful Yeast Expression System for Biologics Production","authors":"Yagmur Unver, Ibrahim Dagci","doi":"10.31083/j.fbe1602019","DOIUrl":"https://doi.org/10.31083/j.fbe1602019","url":null,"abstract":"Komagataella phaffii (K. phaffii) ( Pichia pastoris ), also called biotech yeast, is a yeast species with many applications in the biotechnology and pharmaceutical industries. This methylotrophic yeast has garnered significant interest as a platform for the production of recombinant proteins. Numerous benefits include effective secretory expression that facilitates the easy purification of heterologous proteins, high cell density with rapid growth, post-translational changes, and stable gene expression with integration into the genome. In the last thirty years, K. phaffii has also been refined as an adaptable cell factory that can produce hundreds of biomolecules in a laboratory setting and on an industrial scale. Indeed, over 5000 recombinant proteins have been generated so far using the K. phaffii expression method, which makes up 30% of the total cell protein or 80% of the total released protein. K. phaffii has been used to manufacture more than 70 commercial products in addition to over 300 industrial processes that have been granted licenses. Among these are useful enzymes for industrial biotechnology, including xylanase, mannanase, lipase, and phytase. The others are biopharmaceuticals, which include human serum albumin, insulin, hepatitis B surface antigen, and epidermal growth factor. Compared to other expression systems, this yeast is also considered a special host for synthesizing subunit vaccines, which have recently been supplanted by alternative vaccination types, such as inactivated/killed and live attenuated vaccines. Moreover, efficient production of recombinant proteins is achieved through multi-level optimization methods, such as codon bias, gene dosage, promoters, signal peptides, and environmental factors. Therefore, although K. phaffii expression systems are efficient and simple with clearly established process procedures, it is still necessary to determine the ideal conditions since these vary depending on the target protein to ensure the highest recombinant protein generation. This review addresses the K. phaffii expression system, its importance in industrial and biopharmaceutical protein production, and some bioprocessing and genetic modification strategies for efficient protein production. K. phaffii will eventually continue contributing as a potent expression system in research areas and industrial applications.","PeriodicalId":502751,"journal":{"name":"Frontiers in Bioscience-Elite","volume":"82 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141353001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Mohapatra, Chowdaiah Nickhil, Abhijit Kar, Yogesh Sharma, Sumedha S Deshpande, Manoj Kumar Tripathi, Sadvatha Ramanna Haromuchadi
{"title":"Impact of LAB Fermentation on the Nutrient Content, Amino Acid Profile, and Estimated Glycemic Index of Sorghum, Pearl Millet, and Kodo Millet","authors":"D. Mohapatra, Chowdaiah Nickhil, Abhijit Kar, Yogesh Sharma, Sumedha S Deshpande, Manoj Kumar Tripathi, Sadvatha Ramanna Haromuchadi","doi":"10.31083/j.fbe1602018","DOIUrl":"https://doi.org/10.31083/j.fbe1602018","url":null,"abstract":"","PeriodicalId":502751,"journal":{"name":"Frontiers in Bioscience-Elite","volume":"45 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141358739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dextran Used in Blood Transfusion, Hematology, and Pharmaceuticals: Biosynthesis of Diverse Molecular-Specification-Dextrans in Enzyme-Catalyzed Reactions","authors":"Divakar Dahiya, Poonam Singh Nigam","doi":"10.31083/j.fbe1602017","DOIUrl":"https://doi.org/10.31083/j.fbe1602017","url":null,"abstract":"","PeriodicalId":502751,"journal":{"name":"Frontiers in Bioscience-Elite","volume":"95 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141388227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Joel B. Johnson, Ruslan Timofeev, Anatoliy Kazak, Yurij Grishin, Ludmila Solovyova, Marina Rudenko
{"title":"A Study of the UV Spectral Features in Wine and Their Correlation with Phenolic Constituents","authors":"Joel B. Johnson, Ruslan Timofeev, Anatoliy Kazak, Yurij Grishin, Ludmila Solovyova, Marina Rudenko","doi":"10.31083/j.fbe1602016","DOIUrl":"https://doi.org/10.31083/j.fbe1602016","url":null,"abstract":"","PeriodicalId":502751,"journal":{"name":"Frontiers in Bioscience-Elite","volume":"31 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140969151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thiyagarajan Deborah Winssy, N. O. Gopal, Pandiyan Indiragandhi, Venkatasamy Balasubramani, Rangasamy Anandham
{"title":"Chitinase Producing Gut-Associated Bacteria Affected the Survivability of the Insect Spodoptera frugiperda","authors":"Thiyagarajan Deborah Winssy, N. O. Gopal, Pandiyan Indiragandhi, Venkatasamy Balasubramani, Rangasamy Anandham","doi":"10.31083/j.fbe1602015","DOIUrl":"https://doi.org/10.31083/j.fbe1602015","url":null,"abstract":"","PeriodicalId":502751,"journal":{"name":"Frontiers in Bioscience-Elite","volume":"58 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140971112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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