MD.A. Hossain, Mst. Smrity Khatun, M. Hosen, Zakariya Ibne Sayed, Md. Rafiqul Islam, Md. Kaium Chowdhury, M. Iqbal, Ibrahim Al-Ashkar, Zeki Erden, Çağdaş Can Toprak, A. E. Sabagh, Mohammad Sohidul Islam
Background: Salt stress (SS) has seriously threatened the productivity of pulses including black gram in modern input-intensive farming systems which necessitates finding biologically viable, pro-farmer and environmentally friendly SS ameliorating strategies. Methods: An experiment was conducted to assess three levels of both SS (0, 50 and 100 mM NaCl) and citric acid (CA, 0, 50 and 100 µM) applied as a foliar spray to ameliorate the deleterious effects of SS on black gram (cv. BARI Mash-3). The response variables included plant growth traits like plant height (PH), leaf number (BLPP) and root dry weight (RDW) along with chlorophyll contents (chl a, chl b and tchl), plant water status (relative water content RWC and water retention capacity WRC), grain yield (GY), stover yield (SY), biological yield (BY)) and harvest index (HI) along with nitrogen (N) and protein (P) content of black gram. Result: The results revealed that CA (100 µM) remained unmatched by increasing PH (76.25%), NLPP (37.52%), RDW (83.67%), Chl a (17.80%), Chl b (11.59%), tChl (15.51%), RWC (9.81%) and WRC (26.64%) under highest level of induced SS. The same treatment also surpassed the rest of the doses in terms of grains number per pod (23.89%), 100 grains weight (59.74%), GY (82.86%), SY (59.66%), BY (64.94%) and HI. Moreover, CA accumulated N and P content (29.9%) in the grain under SS conditions. These results indicated that application CA alleviated the adverse effects of SS by triggering the growth, yield and nutritional quality which might be developed as a potent strategy to cope with the declining productivity of black gram in saline environment.
背景：盐胁迫（SS）严重威胁着包括黑糯米在内的豆类作物在现代投入密集型耕作体系中的生产力，因此有必要找到生物可行、有利于农民和环境友好的盐胁迫改善策略。方法：进行了一项实验，以评估三种水平的 SS（0、50 和 100 mM NaCl）和柠檬酸（CA，0、50 和 100 µM）作为叶面喷洒剂，以改善 SS 对黑糯米（BARI Mash-3）的有害影响。反应变量包括植株生长性状，如株高（PH）、叶片数（BLPP）和根干重（RDW），以及叶绿素含量（chl a、chl b 和 tchl）、植株水分状况（相对含水量 RWC 和保水能力 WRC）、黑禾的谷物产量（GY）、秸秆产量（SY）、生物产量（BY））和收获指数（HI），以及氮（N）和蛋白质（P）含量。结果结果表明，在最高诱导 SS 水平下，CA（100 µM）仍能提高 PH (76.25%)、NLPP (37.52%)、RDW (83.67%)、Chl a (17.80%)、Chl b (11.59%)、tChl (15.51%)、RWC (9.81%) 和 WRC (26.64%)。同一处理在每荚粒数（23.89%）、百粒重（59.74%）、GY（82.86%）、SY（59.66%）、BY（64.94%）和 HI 方面也超过了其他剂量。此外，在 SS 条件下，CA 增加了谷粒中的氮和磷含量（29.9%）。这些结果表明，施用 CA 能缓解 SS 的不利影响，促进生长、提高产量和营养质量，可作为应对盐碱环境下黑穗醋栗产量下降的有效策略。
{"title":"Citric Acid Alleviated Salt Stress by Modulating Photosynthetic Pigments, Plant Water Status, Yield and Nutritional Quality of Black gram [Vigna mungo (L.) Hepper]","authors":"MD.A. Hossain, Mst. Smrity Khatun, M. Hosen, Zakariya Ibne Sayed, Md. Rafiqul Islam, Md. Kaium Chowdhury, M. Iqbal, Ibrahim Al-Ashkar, Zeki Erden, Çağdaş Can Toprak, A. E. Sabagh, Mohammad Sohidul Islam","doi":"10.18805/lrf-820","DOIUrl":"https://doi.org/10.18805/lrf-820","url":null,"abstract":"Background: Salt stress (SS) has seriously threatened the productivity of pulses including black gram in modern input-intensive farming systems which necessitates finding biologically viable, pro-farmer and environmentally friendly SS ameliorating strategies. Methods: An experiment was conducted to assess three levels of both SS (0, 50 and 100 mM NaCl) and citric acid (CA, 0, 50 and 100 µM) applied as a foliar spray to ameliorate the deleterious effects of SS on black gram (cv. BARI Mash-3). The response variables included plant growth traits like plant height (PH), leaf number (BLPP) and root dry weight (RDW) along with chlorophyll contents (chl a, chl b and tchl), plant water status (relative water content RWC and water retention capacity WRC), grain yield (GY), stover yield (SY), biological yield (BY)) and harvest index (HI) along with nitrogen (N) and protein (P) content of black gram. Result: The results revealed that CA (100 µM) remained unmatched by increasing PH (76.25%), NLPP (37.52%), RDW (83.67%), Chl a (17.80%), Chl b (11.59%), tChl (15.51%), RWC (9.81%) and WRC (26.64%) under highest level of induced SS. The same treatment also surpassed the rest of the doses in terms of grains number per pod (23.89%), 100 grains weight (59.74%), GY (82.86%), SY (59.66%), BY (64.94%) and HI. Moreover, CA accumulated N and P content (29.9%) in the grain under SS conditions. These results indicated that application CA alleviated the adverse effects of SS by triggering the growth, yield and nutritional quality which might be developed as a potent strategy to cope with the declining productivity of black gram in saline environment.\u0000","PeriodicalId":503097,"journal":{"name":"LEGUME RESEARCH - AN INTERNATIONAL JOURNAL","volume":"17 26","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141925318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Sirisha, S. Pushpavalli, P. Sujatha, S. Vanisri, M. Rajendar Reddy
Background: Seed longevity is a major constraint in soybean seed production. The major focus of this study is to analyze the physiological and molecular changes associated with seed longevity and identify promising germplasm which are good storers for soybean breeding program. Methods: Nineteen genotypes were studied for seed longevity using accelerated ageing test and genetic integrity based on SSR marker data. Genotypes were clustered into distinct groups based on seed morphological and physiological parameters (Mahalanobis D2 analysis). SSR markers for seed longevity were validated in the germplasm. Result: Per cent reduction in germination after accelerated ageing was significantly and positively correlated with traits associated with seed storability such as seed length, seed width, seed thickness and 100 seed weight and negatively correlated with seedling vigour indices. Hence, it would be worthwhile to rely upon these parameters for enhancing the seed storability in soybean. Genetic integrity of the germplasm was evaluated based on SSR markers in accelerated ageing seeds. SSR markers (Satt 285, Satt 534, Satt 538, Satt 281, Satt 162, Satt 631 and Satt 371) revealed significant association for the seed longevity characters such as seed length, seed width, seed thickness and seed weight. Candidate markers (Satt 371, Satt 281, Satt162, Satt 285, Satt 534) which can differentiate the soybean genotypes for storability have been identified in this study. The genotypes were grouped into seven clusters with monogenotypic cluster III (PSPB23) having minimum reduction in germination after accelerated ageing.
背景：种子寿命是大豆种子生产的一个主要限制因素。本研究的重点是分析与种子寿命相关的生理和分子变化，并为大豆育种计划鉴定有潜力的优良贮种种质。研究方法利用加速老化试验和基于 SSR 标记数据的遗传完整性研究了 19 个基因型的种子寿命。根据种子形态和生理参数（Mahalanobis D2 分析）将基因型分为不同的组。在种质中验证了种子寿命的 SSR 标记。结果加速老化后发芽率降低的百分比与种子贮藏性相关性状（如种子长度、种子宽度、种子厚度和 100 粒种子重量）呈显著正相关，而与幼苗活力指数呈负相关。因此，值得依靠这些参数来提高大豆种子的贮藏性。根据加速老化种子中的 SSR 标记评估了种质的遗传完整性。SSR 标记（Satt 285、Satt 534、Satt 538、Satt 281、Satt 162、Satt 631 和 Satt 371）与种子寿命特征（如种子长度、种子宽度、种子厚度和种子重量）有显著关联。本研究确定了可区分大豆耐贮性基因型的候选标记（Satt 371、Satt 281、Satt 162、Satt 285 和 Satt 534）。基因型被分为 7 个群组，其中单基因型群组 III（PSPB23）在加速老化后的发芽率降低幅度最小。
{"title":"Physiological and Molecular Analysis of Soybean Seed Longevity and Validation of Candidate Markers","authors":"P. Sirisha, S. Pushpavalli, P. Sujatha, S. Vanisri, M. Rajendar Reddy","doi":"10.18805/lr-5213","DOIUrl":"https://doi.org/10.18805/lr-5213","url":null,"abstract":"Background: Seed longevity is a major constraint in soybean seed production. The major focus of this study is to analyze the physiological and molecular changes associated with seed longevity and identify promising germplasm which are good storers for soybean breeding program. Methods: Nineteen genotypes were studied for seed longevity using accelerated ageing test and genetic integrity based on SSR marker data. Genotypes were clustered into distinct groups based on seed morphological and physiological parameters (Mahalanobis D2 analysis). SSR markers for seed longevity were validated in the germplasm. Result: Per cent reduction in germination after accelerated ageing was significantly and positively correlated with traits associated with seed storability such as seed length, seed width, seed thickness and 100 seed weight and negatively correlated with seedling vigour indices. Hence, it would be worthwhile to rely upon these parameters for enhancing the seed storability in soybean. Genetic integrity of the germplasm was evaluated based on SSR markers in accelerated ageing seeds. SSR markers (Satt 285, Satt 534, Satt 538, Satt 281, Satt 162, Satt 631 and Satt 371) revealed significant association for the seed longevity characters such as seed length, seed width, seed thickness and seed weight. Candidate markers (Satt 371, Satt 281, Satt162, Satt 285, Satt 534) which can differentiate the soybean genotypes for storability have been identified in this study. The genotypes were grouped into seven clusters with monogenotypic cluster III (PSPB23) having minimum reduction in germination after accelerated ageing.","PeriodicalId":503097,"journal":{"name":"LEGUME RESEARCH - AN INTERNATIONAL JOURNAL","volume":"39 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141102134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Bhattacharya, S. Roy, M. Murmu, S. Gorai, A. Das, J. Banerjee, S. Gupta
Background: Micronutrient malnutrition is a severe peril for wellbeing of humankind, which can be holistically addressed through genetic biofortification. Lentil, proclaim as poor man’s meat can hold a great promise in global biofortification programme. The present study was designed to appraise genetic variability for Fe and Zn content and elucidate the role of Genotype × Environment interaction for delimitation of micronutrient enriched stable lentil genotypes integrating HA-GGE biplot with REML/BLUP. Methods: Grain Fe and Zn content of 44 lentil genotypes grown at three different locations of West Bengal during two consecutive years were estimated for deciphering the G × E interaction combining HA-GGE and REML/BLUP. Result: Results revealed substantial genetic variability for Fe (48.07 to 107.45 mg kg-1) and Zn (38.72 to 60.07 mg kg-1) in 44 lentil genotypes with significant influence of environment and GE interaction. The present study precisely detected ILL-10123 and VL-156 as the ‘ideal’ genotypes for Fe and Zn content, respectively in addition to non-redundant testing location. Identified genotypes and testing location aid in global biofortification programme for upscaling micronutrient concentration in lentil.
{"title":"Genotype × Environment Interaction for Appraisal of Iron and Zinc Rich Stable Genotypes in Lentil (Lens culinaris Medik.)","authors":"S. Bhattacharya, S. Roy, M. Murmu, S. Gorai, A. Das, J. Banerjee, S. Gupta","doi":"10.18805/lr-5205","DOIUrl":"https://doi.org/10.18805/lr-5205","url":null,"abstract":"Background: Micronutrient malnutrition is a severe peril for wellbeing of humankind, which can be holistically addressed through genetic biofortification. Lentil, proclaim as poor man’s meat can hold a great promise in global biofortification programme. The present study was designed to appraise genetic variability for Fe and Zn content and elucidate the role of Genotype × Environment interaction for delimitation of micronutrient enriched stable lentil genotypes integrating HA-GGE biplot with REML/BLUP. Methods: Grain Fe and Zn content of 44 lentil genotypes grown at three different locations of West Bengal during two consecutive years were estimated for deciphering the G × E interaction combining HA-GGE and REML/BLUP. Result: Results revealed substantial genetic variability for Fe (48.07 to 107.45 mg kg-1) and Zn (38.72 to 60.07 mg kg-1) in 44 lentil genotypes with significant influence of environment and GE interaction. The present study precisely detected ILL-10123 and VL-156 as the ‘ideal’ genotypes for Fe and Zn content, respectively in addition to non-redundant testing location. Identified genotypes and testing location aid in global biofortification programme for upscaling micronutrient concentration in lentil.\u0000","PeriodicalId":503097,"journal":{"name":"LEGUME RESEARCH - AN INTERNATIONAL JOURNAL","volume":"20 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141106386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. G. K. Reddy, T.V. Prasad, K. Sreedevi Shankar, Ms. Pushpanjali, N. Jyothilakshmi, K. Salini, R. Ramesh Babu, Vinod Kumar Singh, Jagati Yadagiri
Background: The research was conducted at the ICAR-CRIDA Hayatnagar Research Farm in Hyderabad, India. The plant material consists of tamarind trees planted in 1998 with 5m spacing to improve the morphological and reproductive characteristics of elite genotypes as well as quality and biochemical characters among the twenty tamarind accessions maintained at the research farm. The experiment was started in 1998 and observations were taken over the fruiting season of 2021-2022 with twenty tamarind accessions; the experiments were established in a randomized block design. The trees were identified for their consistent health and development. Methods: Biometric observations mainly average number of flowers per inflorescence, average number of inflorescence per branch, average number of branches per tree, average fruit weight (g), average yield per plant (kg), fruit, pulp, seed weights as well as shell, fiber, fruit, number of normal seeds per pod as well as damaged number of seeds per pod, Carbohydrates g/ 100 g, Polyphenols g/100 g, Anthocyanin mg/100 g, % Antioxidant activity and % Tartaric acid were recorded and analyzed statistically. Result: Significant differences among the tamarind accessions evaluated, NZB(S), Hasanur #5, Salem 132, NTI-14 and SMG-3 recorded the highest values in all the growth, pod and yield characters. NZB(S) recorded the highest number of flowers per inflorescence (14.62) while Hasanur # 5 recorded the highest number of inflorescence per branch (13.87). In yield attributes, NZB(S) recorded the highest average yield per plant (kg) (15.72) followed by Hasanur #5 (15.09), Salem 132 (14.81) and NTI-14 (14.65). The results revealed that NZB(S) showed the highest mean performance in terms of growth, yield and quality characters. The best performing accessions are being multiplied through vegetative propagation methods for planting on large scale in different locations.
{"title":"Morpho-biochemical Characterization of Tamarind (Tamarindus indica L.)","authors":"A. G. K. Reddy, T.V. Prasad, K. Sreedevi Shankar, Ms. Pushpanjali, N. Jyothilakshmi, K. Salini, R. Ramesh Babu, Vinod Kumar Singh, Jagati Yadagiri","doi":"10.18805/lr-5274","DOIUrl":"https://doi.org/10.18805/lr-5274","url":null,"abstract":"Background: The research was conducted at the ICAR-CRIDA Hayatnagar Research Farm in Hyderabad, India. The plant material consists of tamarind trees planted in 1998 with 5m spacing to improve the morphological and reproductive characteristics of elite genotypes as well as quality and biochemical characters among the twenty tamarind accessions maintained at the research farm. The experiment was started in 1998 and observations were taken over the fruiting season of 2021-2022 with twenty tamarind accessions; the experiments were established in a randomized block design. The trees were identified for their consistent health and development. Methods: Biometric observations mainly average number of flowers per inflorescence, average number of inflorescence per branch, average number of branches per tree, average fruit weight (g), average yield per plant (kg), fruit, pulp, seed weights as well as shell, fiber, fruit, number of normal seeds per pod as well as damaged number of seeds per pod, Carbohydrates g/ 100 g, Polyphenols g/100 g, Anthocyanin mg/100 g, % Antioxidant activity and % Tartaric acid were recorded and analyzed statistically. Result: Significant differences among the tamarind accessions evaluated, NZB(S), Hasanur #5, Salem 132, NTI-14 and SMG-3 recorded the highest values in all the growth, pod and yield characters. NZB(S) recorded the highest number of flowers per inflorescence (14.62) while Hasanur # 5 recorded the highest number of inflorescence per branch (13.87). In yield attributes, NZB(S) recorded the highest average yield per plant (kg) (15.72) followed by Hasanur #5 (15.09), Salem 132 (14.81) and NTI-14 (14.65). The results revealed that NZB(S) showed the highest mean performance in terms of growth, yield and quality characters. The best performing accessions are being multiplied through vegetative propagation methods for planting on large scale in different locations.\u0000","PeriodicalId":503097,"journal":{"name":"LEGUME RESEARCH - AN INTERNATIONAL JOURNAL","volume":"32 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141105349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Divya, M. Prahadeeswaran, M. Malarkodi, K. Uma, M. Kavitha, K.S. Vishwa
Background: The multifaceted importance of pulses in India’s agricultural landscape is explored along with its implications particularly the surge in pulse exports to regions such as the Middle East, Southeast Asia and Africa. By highlighting India’s pivotal role as a global pulse producer and exporter, shedding light on its impact on foreign exchange earnings, employment generation, comprehensive analysis and overall economic growth is attempted. Two key objectives, relating to pulse production, import and export trends, the direction of pulse trade from India to major importing countries, through Markov Chain Analysis, market dynamics are uncovered. Methods: The data on pulses production and export in terms of quantity and value in India were collected from secondary sources. Result: Pulse production remained relatively stable with moderate variability, exports experienced significant fluctuations, particularly in pulse quantities and values. Compound Annual Growth Rate (CAGR) analysis confirms consistent production growth, with pulses showing positive CAGR figures. Impact of interaction between area and yield was significant as compared to area and yield independently. Pulse exports saw steady growth in both quantity and value. Further, The Markov chain analysis underscored Pakistan’s stability as a primary export destination, while Bangladesh, the UAE and others exhibited varying degrees of export retention probabilities. Overall, the data reflects a stable domestic production environment and an increasingly dynamic international pulse export market for India.
{"title":"Comprehensive Analysis of Production and Import Dependency of Pulses in India","authors":"K. Divya, M. Prahadeeswaran, M. Malarkodi, K. Uma, M. Kavitha, K.S. Vishwa","doi":"10.18805/lr-5294","DOIUrl":"https://doi.org/10.18805/lr-5294","url":null,"abstract":"Background: The multifaceted importance of pulses in India’s agricultural landscape is explored along with its implications particularly the surge in pulse exports to regions such as the Middle East, Southeast Asia and Africa. By highlighting India’s pivotal role as a global pulse producer and exporter, shedding light on its impact on foreign exchange earnings, employment generation, comprehensive analysis and overall economic growth is attempted. Two key objectives, relating to pulse production, import and export trends, the direction of pulse trade from India to major importing countries, through Markov Chain Analysis, market dynamics are uncovered. Methods: The data on pulses production and export in terms of quantity and value in India were collected from secondary sources. Result: Pulse production remained relatively stable with moderate variability, exports experienced significant fluctuations, particularly in pulse quantities and values. Compound Annual Growth Rate (CAGR) analysis confirms consistent production growth, with pulses showing positive CAGR figures. Impact of interaction between area and yield was significant as compared to area and yield independently. Pulse exports saw steady growth in both quantity and value. Further, The Markov chain analysis underscored Pakistan’s stability as a primary export destination, while Bangladesh, the UAE and others exhibited varying degrees of export retention probabilities. Overall, the data reflects a stable domestic production environment and an increasingly dynamic international pulse export market for India.\u0000","PeriodicalId":503097,"journal":{"name":"LEGUME RESEARCH - AN INTERNATIONAL JOURNAL","volume":"121 44","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141115393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. A. Bagheripour, H. H. Sharifabad, Ahmad Mehraban, H. Ganjali
Background: Yield gap is distance between potential yield and attainable yield (yield gap= potential yield-attainable yield) in order to increase the crop yield and the goal of sustainable agriculture. Methods: This study was conducted in Bam, Narmashir and Fahraj counties in 219 separate farms (79 farms in Bam, 72 farms in Narmashir and 68 farms in Fahraj) during the years 2019 and 2020 to investigate the yield gap of wheat and determine the limiting factors and their contributions to yield reduction, using the comparative performance analysis (CPA) method. All information related to soil factors, management factors, climatic factors and agronomic traits (53 variables) were measured and recorded. The relationship between wheat yield and all variables was examined using a stepwise multiple regression method. A production model was determined for soil and management factors. Result: The results showed a yield gap between actual yield and potential yield in the regions of Bam, Narmashir and Fahraj, which were 1770.8, 1817.61 and 1605 kg per hectare, respectively. Since the actual yield in these regions was 4248, 4228 and 3161 kg per hectare, the yield gap was observed to be 33.6%, 30% and 29.4%, respectively. The effective factors in reducing the yield in the Bam region included water shortage (19.48%), nitrogen deficiency (16.09%), delayed planting (15.40%), soil available phosphorus deficiency (10.14%), weed density (10.8%), soil organic carbon (13.59%) and inappropriate plant density (14.47%). In the Narmashir region, water shortage (26.68%), nitrogen deficiency (18.86%), soil organic carbon (11.78%), inappropriate plant density (12.43%), wheat aphid (19.58%) and weed density (10.64%) caused yield reduction. In the Fahraj region, water shortage (20.65%), nitrogen deficiency (19.62%), delayed planting (9.44%), soil available phosphorus deficiency (8.45%), weed density (12%), soil salinity (12.80%) and black stem disease (17%) caused a decrease in wheat yield.
{"title":"Estimating Yield Gap of Wheat (Triticum aestivum L.) using the Comparative Performance Analysis (CPA) Method in the Bam, Narmashir and Fahraj Counties of Kerman Province","authors":"M. A. Bagheripour, H. H. Sharifabad, Ahmad Mehraban, H. Ganjali","doi":"10.18805/lrf-777","DOIUrl":"https://doi.org/10.18805/lrf-777","url":null,"abstract":"Background: Yield gap is distance between potential yield and attainable yield (yield gap= potential yield-attainable yield) in order to increase the crop yield and the goal of sustainable agriculture. Methods: This study was conducted in Bam, Narmashir and Fahraj counties in 219 separate farms (79 farms in Bam, 72 farms in Narmashir and 68 farms in Fahraj) during the years 2019 and 2020 to investigate the yield gap of wheat and determine the limiting factors and their contributions to yield reduction, using the comparative performance analysis (CPA) method. All information related to soil factors, management factors, climatic factors and agronomic traits (53 variables) were measured and recorded. The relationship between wheat yield and all variables was examined using a stepwise multiple regression method. A production model was determined for soil and management factors. Result: The results showed a yield gap between actual yield and potential yield in the regions of Bam, Narmashir and Fahraj, which were 1770.8, 1817.61 and 1605 kg per hectare, respectively. Since the actual yield in these regions was 4248, 4228 and 3161 kg per hectare, the yield gap was observed to be 33.6%, 30% and 29.4%, respectively. The effective factors in reducing the yield in the Bam region included water shortage (19.48%), nitrogen deficiency (16.09%), delayed planting (15.40%), soil available phosphorus deficiency (10.14%), weed density (10.8%), soil organic carbon (13.59%) and inappropriate plant density (14.47%). In the Narmashir region, water shortage (26.68%), nitrogen deficiency (18.86%), soil organic carbon (11.78%), inappropriate plant density (12.43%), wheat aphid (19.58%) and weed density (10.64%) caused yield reduction. In the Fahraj region, water shortage (20.65%), nitrogen deficiency (19.62%), delayed planting (9.44%), soil available phosphorus deficiency (8.45%), weed density (12%), soil salinity (12.80%) and black stem disease (17%) caused a decrease in wheat yield.\u0000","PeriodicalId":503097,"journal":{"name":"LEGUME RESEARCH - AN INTERNATIONAL JOURNAL","volume":"140 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141114676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. J. Abhishek, Kuldeep Tripathi, K. Kalaiponmani, D.D. Deepika, Ruchi Sharma, A. Maurya, V. C. Chalam
Background: Bean common mosaic disease (BCMD) caused by Bean common mosaic virus (BCMV) has been regarded as one of the most important seed-borne and aphid vector transmitted destructive disease of cowpea not only affecting its yield but also reducing its grain quality globally. Since the dawn of crop improvement, germplasm served as the source of resistance for various biotic stresses. Currently, host plant resistance is the best, reliable, economic and environmental friendly practice of virus disease management. The objective of this study was to assess the response of diverse cowpea germplasm against BCMV under natural and controlled conditions followed by serological and molecular detection. Methods: In this study, 85 cowpea germplasm accessions and one known BCMV susceptible (C152) and one resistant (CP55) varieties are evaluated for their response to the BCMD in augmented block design (ABD). The reaction of cowpea accessions were assessed using percent disease incidence (PDI). Transmission Electron microscopy (TEM), Direct Antigen Coating-Enzyme-linked Immunosorbent Assay (DAC-ELISA) and Reverse Transcription-Polymerase Chain Reaction (RT-PCR) were used for confirming the BCMV. Result: Natural and controlled condition screening data analysis revealed a significant difference among diverse cowpea germplasm for BCMV diseases incidence. Based on field screening at Ranchi and New Delhi (2019 and 2020) and artificial confirmation. 24 out of 85 accessions were found to be immune and confirmed through DAC-ELISA and RT-PCR. As a consequence these accessions could be a potential new source of disease resistance for BCMV in breeding programs.Further, DAC ELISA of seed coat and Embryo revealed the presence of virus in 5% and 15% of samples tested. Accession IC418505 recorded highest disease incidence (40.0%).
{"title":"Germplasm Screening for Identification of BCMV Resistance Sources from Diverse Cowpea [Vigna unguiculata (L.) Walp.] Germplasm using Serological and Molecular Diagnostics","authors":"G. J. Abhishek, Kuldeep Tripathi, K. Kalaiponmani, D.D. Deepika, Ruchi Sharma, A. Maurya, V. C. Chalam","doi":"10.18805/lr-5169","DOIUrl":"https://doi.org/10.18805/lr-5169","url":null,"abstract":"Background: Bean common mosaic disease (BCMD) caused by Bean common mosaic virus (BCMV) has been regarded as one of the most important seed-borne and aphid vector transmitted destructive disease of cowpea not only affecting its yield but also reducing its grain quality globally. Since the dawn of crop improvement, germplasm served as the source of resistance for various biotic stresses. Currently, host plant resistance is the best, reliable, economic and environmental friendly practice of virus disease management. The objective of this study was to assess the response of diverse cowpea germplasm against BCMV under natural and controlled conditions followed by serological and molecular detection. Methods: In this study, 85 cowpea germplasm accessions and one known BCMV susceptible (C152) and one resistant (CP55) varieties are evaluated for their response to the BCMD in augmented block design (ABD). The reaction of cowpea accessions were assessed using percent disease incidence (PDI). Transmission Electron microscopy (TEM), Direct Antigen Coating-Enzyme-linked Immunosorbent Assay (DAC-ELISA) and Reverse Transcription-Polymerase Chain Reaction (RT-PCR) were used for confirming the BCMV. Result: Natural and controlled condition screening data analysis revealed a significant difference among diverse cowpea germplasm for BCMV diseases incidence. Based on field screening at Ranchi and New Delhi (2019 and 2020) and artificial confirmation. 24 out of 85 accessions were found to be immune and confirmed through DAC-ELISA and RT-PCR. As a consequence these accessions could be a potential new source of disease resistance for BCMV in breeding programs.Further, DAC ELISA of seed coat and Embryo revealed the presence of virus in 5% and 15% of samples tested. Accession IC418505 recorded highest disease incidence (40.0%).\u0000","PeriodicalId":503097,"journal":{"name":"LEGUME RESEARCH - AN INTERNATIONAL JOURNAL","volume":"59 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141114083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Fenugreek (Trigonella foenum-graecum L.) family: fabaceae, locally known as ‘Methi’ is a self pollinated leguminous spice crop. The plant originated in India and Northern Africa. In India, it is commonly consumed a condiment and used medicinally as a lactation stimulant. There are numerous other folkloric uses of fenugreek, for example, in the treatment of indigestion and baldness. The crop has immense medicinal value and is a good source of vitamins, protein and essential oils. The leaves are used as a vegetable and the seeds as spice for adding medicinal value and flavor to the human food and also forage for cattle. Methods: The present investigation was conducted during 2017-18 and 2018-2019 under laboratory and field conditions in the Department of Seed Science and Technology, Dr. YS Parmar University of Horticulture and Forestry, Nauni, Solan (HP). In both the years, ten bioresources based seed production modules were tested under field conditions. The field experiment comparised of 10 bioresource based seed production modules with one untreated control was laid out in Randomized Complete Block Design (RCBD) with three replications. Result: It can be concluded from the present investigation that bioresource based seed production Module-5 consist of seed treatment with Rhizobium strain-B2@ 100 ml/kg seeds, soil application of Trichoderma viride mixed with FYM @ 2 L/20 kg and arbuscular mycorrhizal (AM) fungi @ 2 kg of soil inoculum/m2 and foliar application of water extracts of garlic (Allium sativum) and drake (Melia azedarach) @ 5% is an effective treatment module which improved plant growth, pod yield, seed yield and reduced the incidence of downy mildew as compared to untreated control.
{"title":"Studies on Bioresource based Seed Production Modules in Fenugreek (Trigonella foenum graecum L.)","authors":"Meghana Singh, N. Bharat","doi":"10.18805/lr-4973","DOIUrl":"https://doi.org/10.18805/lr-4973","url":null,"abstract":"Background: Fenugreek (Trigonella foenum-graecum L.) family: fabaceae, locally known as ‘Methi’ is a self pollinated leguminous spice crop. The plant originated in India and Northern Africa. In India, it is commonly consumed a condiment and used medicinally as a lactation stimulant. There are numerous other folkloric uses of fenugreek, for example, in the treatment of indigestion and baldness. The crop has immense medicinal value and is a good source of vitamins, protein and essential oils. The leaves are used as a vegetable and the seeds as spice for adding medicinal value and flavor to the human food and also forage for cattle. Methods: The present investigation was conducted during 2017-18 and 2018-2019 under laboratory and field conditions in the Department of Seed Science and Technology, Dr. YS Parmar University of Horticulture and Forestry, Nauni, Solan (HP). In both the years, ten bioresources based seed production modules were tested under field conditions. The field experiment comparised of 10 bioresource based seed production modules with one untreated control was laid out in Randomized Complete Block Design (RCBD) with three replications. Result: It can be concluded from the present investigation that bioresource based seed production Module-5 consist of seed treatment with Rhizobium strain-B2@ 100 ml/kg seeds, soil application of Trichoderma viride mixed with FYM @ 2 L/20 kg and arbuscular mycorrhizal (AM) fungi @ 2 kg of soil inoculum/m2 and foliar application of water extracts of garlic (Allium sativum) and drake (Melia azedarach) @ 5% is an effective treatment module which improved plant growth, pod yield, seed yield and reduced the incidence of downy mildew as compared to untreated control.\u0000","PeriodicalId":503097,"journal":{"name":"LEGUME RESEARCH - AN INTERNATIONAL JOURNAL","volume":"43 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141113533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T.N. Tiwari, D. Dutta, Kalpana Tiwari, S.J. Satheesh Naik
Background: The cold stress adversely affects the growth, survival and reproductive capacity of plants if the minimum temperature falls below 5°C. Information’s regarding cold stress and its impact on morphological characters, physio-biochemical constituents and yield attributes in cold tolerant and susceptible pigeon pea genotypes are very limited. Keeping the above facts in to consideration, the experiments were conducted. Methods: Field experiments were conducted during 2018-19 and 2019-20 with 34 genotypes of pigeon pea including checks (NDA-1, Bahar, IPA203, NDA-2, MAL-13, ICP2275, IPACT-2, MA-6, IPACT-3, IPACT-24, Rajendra Arhar, IPA15F, Dholi-D, JBT46/27, IPACT-6, IPACT-14, IPAD1-17, IPAC 68, IPACT 22, ICPL 7035 IPA19-101, IPA19-102, IPA19-103, IPA19-104, IPA19-105, IPA19-106, IPA19-107, IPA19-108, IPA19-109, IPA19-110, IPA19-111, IPA19-112, IPA19-113, IPA19-114) in RBD at ICAR-IIPR, Kanpur. Morphological and yield Characters including plant height, scoring of cold injury in exposed plants and Number of flower drop and pod set during the cold period in tagged plants were recorded. In biochemical/ crop efficiency parameters viz: Nitrogen balance index, chlorophyll, flavonols, anthocyanin’s and NDVI were measured. The photosynthetic rate, stomatal conductance, and transpiration rate were measured at the pod formation stage. Anti-oxidant enzymes activity was assayed as per standard methods. Meteorological observations during Cold stress were recorded. Result: Based on flower/pod drop and retention the genotypes like IPA 15F, Dholi dwarf, JBT46/27, IPACT-6, IPACT-14, IPAC-1-17, IPACT-68, IPACT-22, IPA 19-101, IPA 19-102, IPA 19-103, IPA 19-104, IPA 19-105, IPA 19-107, IPA 19-108, IPA 19-109, IPA 19-110, IPA 19-111 and IPA 19-113 were found susceptible to cold stress and retaining an average 51.67 % flowers/pods. Genotypes like NDA-2, MAL-13, ICP2275, IPACT-2, MA-6, IPACT-3, IPACT-24, Rajendra arhar, IPA19-106, IPA19-112 and IPA19-114 have retained more than an average of 67.48% flowers/pods are considered tolerant to cold stress. Amongst the tolerant group NDA-2, MAL-13, ICP2275, IPACT-2, IPA19-106, IPA19-112 retained the flower/pod more than the average of check genotypes (67.57%). Biochemical/plant efficiency parameters were superior in tolerant group over susceptible group.
{"title":"Physiological Characterization of Cold Tolerant and Susceptible Pigeon Pea [Cajanus cajan (L.) Millsp.] Genotypes","authors":"T.N. Tiwari, D. Dutta, Kalpana Tiwari, S.J. Satheesh Naik","doi":"10.18805/lr-5226","DOIUrl":"https://doi.org/10.18805/lr-5226","url":null,"abstract":"Background: The cold stress adversely affects the growth, survival and reproductive capacity of plants if the minimum temperature falls below 5°C. Information’s regarding cold stress and its impact on morphological characters, physio-biochemical constituents and yield attributes in cold tolerant and susceptible pigeon pea genotypes are very limited. Keeping the above facts in to consideration, the experiments were conducted. Methods: Field experiments were conducted during 2018-19 and 2019-20 with 34 genotypes of pigeon pea including checks (NDA-1, Bahar, IPA203, NDA-2, MAL-13, ICP2275, IPACT-2, MA-6, IPACT-3, IPACT-24, Rajendra Arhar, IPA15F, Dholi-D, JBT46/27, IPACT-6, IPACT-14, IPAD1-17, IPAC 68, IPACT 22, ICPL 7035 IPA19-101, IPA19-102, IPA19-103, IPA19-104, IPA19-105, IPA19-106, IPA19-107, IPA19-108, IPA19-109, IPA19-110, IPA19-111, IPA19-112, IPA19-113, IPA19-114) in RBD at ICAR-IIPR, Kanpur. Morphological and yield Characters including plant height, scoring of cold injury in exposed plants and Number of flower drop and pod set during the cold period in tagged plants were recorded. In biochemical/ crop efficiency parameters viz: Nitrogen balance index, chlorophyll, flavonols, anthocyanin’s and NDVI were measured. The photosynthetic rate, stomatal conductance, and transpiration rate were measured at the pod formation stage. Anti-oxidant enzymes activity was assayed as per standard methods. Meteorological observations during Cold stress were recorded. Result: Based on flower/pod drop and retention the genotypes like IPA 15F, Dholi dwarf, JBT46/27, IPACT-6, IPACT-14, IPAC-1-17, IPACT-68, IPACT-22, IPA 19-101, IPA 19-102, IPA 19-103, IPA 19-104, IPA 19-105, IPA 19-107, IPA 19-108, IPA 19-109, IPA 19-110, IPA 19-111 and IPA 19-113 were found susceptible to cold stress and retaining an average 51.67 % flowers/pods. Genotypes like NDA-2, MAL-13, ICP2275, IPACT-2, MA-6, IPACT-3, IPACT-24, Rajendra arhar, IPA19-106, IPA19-112 and IPA19-114 have retained more than an average of 67.48% flowers/pods are considered tolerant to cold stress. Amongst the tolerant group NDA-2, MAL-13, ICP2275, IPACT-2, IPA19-106, IPA19-112 retained the flower/pod more than the average of check genotypes (67.57%). Biochemical/plant efficiency parameters were superior in tolerant group over susceptible group.\u0000","PeriodicalId":503097,"journal":{"name":"LEGUME RESEARCH - AN INTERNATIONAL JOURNAL","volume":"134 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141114776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wenna Fan, Mengyao Zhang, Wenfeng Ma, Yixin Yang, Yaqi Shi
Background: Phosphatidyl ethanolamine binding protein (PEBP) plays an important role in the formation of flowers. To identify the PEBP gene family of alfalfa in blossom, the PEBP gene family was selected from the transcriptome database of alfalfa with bioinformatics analysis, for their protein properties, functional domains, evolutionary relationships and expression patterns. Methods: A BLASTP search using Arabidopsis PEBP sequences as a query was performed against alfalfa proteome with a minimum identity of 75% and E-value of 1E-5. BLASTP search resulted in 17 protein candidates. Finally, we obtained a consensus list of ten proteins from the HMMER and BLASTP search. Redundant sequences were removed using CD-HIT which resulted in a total of nine PEBP candidates in alfalfa. Result: The results showed that there was a total of nine PEBP candidates in alfalfa, which could be divided into three sub-gene families, such as TFL1-like, MFT-like and FT-like groups. Chromosomal location analyses showed that nine PEBP members are distributed on five of the eight alfalfa chromosomes with chromosome 7 having the largest number of PEBP members (four members) while only three alfalfa PEBP proteins were annotated as MsFTa1, MsFTa2 and MsFTc. In addition, the motifs of this gene family were conservative with few differences in the number and types of conservative motifs. MsFTa1 and MsFTa2 are two strong candidates for florigen in alfalfa, while MsG0480024000.01 is a candidate which functions as a floral repressory.
{"title":"Identification and Analysis of Phosphatidyl Ethanolamine Binding Protein (PEBP) Family Gene in Alfalfa","authors":"Wenna Fan, Mengyao Zhang, Wenfeng Ma, Yixin Yang, Yaqi Shi","doi":"10.18805/lrf-796","DOIUrl":"https://doi.org/10.18805/lrf-796","url":null,"abstract":"Background: Phosphatidyl ethanolamine binding protein (PEBP) plays an important role in the formation of flowers. To identify the PEBP gene family of alfalfa in blossom, the PEBP gene family was selected from the transcriptome database of alfalfa with bioinformatics analysis, for their protein properties, functional domains, evolutionary relationships and expression patterns. Methods: A BLASTP search using Arabidopsis PEBP sequences as a query was performed against alfalfa proteome with a minimum identity of 75% and E-value of 1E-5. BLASTP search resulted in 17 protein candidates. Finally, we obtained a consensus list of ten proteins from the HMMER and BLASTP search. Redundant sequences were removed using CD-HIT which resulted in a total of nine PEBP candidates in alfalfa. Result: The results showed that there was a total of nine PEBP candidates in alfalfa, which could be divided into three sub-gene families, such as TFL1-like, MFT-like and FT-like groups. Chromosomal location analyses showed that nine PEBP members are distributed on five of the eight alfalfa chromosomes with chromosome 7 having the largest number of PEBP members (four members) while only three alfalfa PEBP proteins were annotated as MsFTa1, MsFTa2 and MsFTc. In addition, the motifs of this gene family were conservative with few differences in the number and types of conservative motifs. MsFTa1 and MsFTa2 are two strong candidates for florigen in alfalfa, while MsG0480024000.01 is a candidate which functions as a floral repressory.\u0000","PeriodicalId":503097,"journal":{"name":"LEGUME RESEARCH - AN INTERNATIONAL JOURNAL","volume":"44 188","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140708282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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