Pub Date : 2023-12-13DOI: 10.3390/proceedings2023092076
Henrik Grénman
{"title":"Sustainable Chemistry through Catalysis and Process Intensification","authors":"Henrik Grénman","doi":"10.3390/proceedings2023092076","DOIUrl":"https://doi.org/10.3390/proceedings2023092076","url":null,"abstract":"","PeriodicalId":504549,"journal":{"name":"International Conference EcoBalt 2023 \"Chemicals & Environment\"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139180908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-30DOI: 10.3390/proceedings2023092058
Mahendra K. Mohan, Harleen Kaur, Ella Duvanova, Merilin Rosenberg, Marcos Dahlem, Angela Ivask, J. Raimundo, Tiit Lukk, Yevgen A. Karpichev
{"title":"Synthesis and Antibacterial Properties of Lignin-Based Quaternary Ammonium and Phosphonium Salts","authors":"Mahendra K. Mohan, Harleen Kaur, Ella Duvanova, Merilin Rosenberg, Marcos Dahlem, Angela Ivask, J. Raimundo, Tiit Lukk, Yevgen A. Karpichev","doi":"10.3390/proceedings2023092058","DOIUrl":"https://doi.org/10.3390/proceedings2023092058","url":null,"abstract":"","PeriodicalId":504549,"journal":{"name":"International Conference EcoBalt 2023 \"Chemicals & Environment\"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139200739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-30DOI: 10.3390/proceedings2023092068
Helen Sepman, Pilleriin Peets, Lisa Jonsson, Louise Malm, M. Posselt, Matthew MacLeod, Jon W. Martin, Magnus Breitholtz, Michael S McLachlan, A. Kruve
{"title":"Machine Learning Tools Can Pinpoint High-Risk Water Pollutants","authors":"Helen Sepman, Pilleriin Peets, Lisa Jonsson, Louise Malm, M. Posselt, Matthew MacLeod, Jon W. Martin, Magnus Breitholtz, Michael S McLachlan, A. Kruve","doi":"10.3390/proceedings2023092068","DOIUrl":"https://doi.org/10.3390/proceedings2023092068","url":null,"abstract":"","PeriodicalId":504549,"journal":{"name":"International Conference EcoBalt 2023 \"Chemicals & Environment\"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139207231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-30DOI: 10.3390/proceedings2023092066
Giuseppe Leonardo Auditano, Karolin Kullison, T. Kaambre, K. Tepp
. The aim of this project was to investigate whether the pesticides Glyphosate, Glyphosate-based Roundup, Boscalid, and NeemAzal alter the energy metabolism of human intestinal cells. The study analyses if prolonged exposure and different growth environments increase the sensitivity of Caco-2 cells to pesticides. The rate of oxygen consumption based on electron flow through individual respiratory chain complexes and the overall oxygen consumption rate of the respiratory chain were analyzed using the method of high-resolution respirometry. The results demonstrated that lower concentrations of pesticides, which do not affected cells in the short term, significantly decreased cell viability with prolonged use. The experiments also showed that, in a plasma-like medium, similar to physiological conditions, the toxic effect of pesticides is higher or equivalent to that observed in cells grown in a regular medium. Analysis of mitochondrial oxidative phosphorylation revealed a significant decrease in the oxygen consumption rate through the electron transport chain at concentrations reducing cell viability by 20% for all pesticides. At lower pesticide concentrations reducing viability by up to 10%, the effect was detectable only for Boscalid and Roundup. The results of the study confirm that commercially available pesticide Roundup, along with its accompanying additives, exhibits stronger toxic effects than the declared active ingredient Glyphosate alone. The results of the study indicate that low pesticide concentrations, which have no immediate impact, may exert toxic effects over a longer period, and this influence should be studied in a plasma-like medium.
{"title":"The Effects of Pesticides on the Bioenergetics of Intestinal Cells","authors":"Giuseppe Leonardo Auditano, Karolin Kullison, T. Kaambre, K. Tepp","doi":"10.3390/proceedings2023092066","DOIUrl":"https://doi.org/10.3390/proceedings2023092066","url":null,"abstract":". The aim of this project was to investigate whether the pesticides Glyphosate, Glyphosate-based Roundup, Boscalid, and NeemAzal alter the energy metabolism of human intestinal cells. The study analyses if prolonged exposure and different growth environments increase the sensitivity of Caco-2 cells to pesticides. The rate of oxygen consumption based on electron flow through individual respiratory chain complexes and the overall oxygen consumption rate of the respiratory chain were analyzed using the method of high-resolution respirometry. The results demonstrated that lower concentrations of pesticides, which do not affected cells in the short term, significantly decreased cell viability with prolonged use. The experiments also showed that, in a plasma-like medium, similar to physiological conditions, the toxic effect of pesticides is higher or equivalent to that observed in cells grown in a regular medium. Analysis of mitochondrial oxidative phosphorylation revealed a significant decrease in the oxygen consumption rate through the electron transport chain at concentrations reducing cell viability by 20% for all pesticides. At lower pesticide concentrations reducing viability by up to 10%, the effect was detectable only for Boscalid and Roundup. The results of the study confirm that commercially available pesticide Roundup, along with its accompanying additives, exhibits stronger toxic effects than the declared active ingredient Glyphosate alone. The results of the study indicate that low pesticide concentrations, which have no immediate impact, may exert toxic effects over a longer period, and this influence should be studied in a plasma-like medium.","PeriodicalId":504549,"journal":{"name":"International Conference EcoBalt 2023 \"Chemicals & Environment\"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139198024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-29DOI: 10.3390/proceedings2023092060
Urška Šunta, P. Trebše, B. Poljšak, M. Kralj
{"title":"Chasing Pollutants Concerning Public Health: From Food to Smoke","authors":"Urška Šunta, P. Trebše, B. Poljšak, M. Kralj","doi":"10.3390/proceedings2023092060","DOIUrl":"https://doi.org/10.3390/proceedings2023092060","url":null,"abstract":"","PeriodicalId":504549,"journal":{"name":"International Conference EcoBalt 2023 \"Chemicals & Environment\"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139213365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-29DOI: 10.3390/proceedings2023092064
Vladyslav Dovhalyuk, Amanpreet Kaur, Weifeng Lin, Ioanna Tsiara, Sydney Mwasambu, Daniel Globisch
One of the most exciting scientific developments in the past decade has been the realization that gut microbiota profoundly impact human physiology. The complex consortium of trillions of microbes possesses a wide range of metabolic activity. Due to the link to disease development from metagenomic analysis the targeted investigation of their metabolism represents a tremendous potential for the discovery of biomarkers and bioactive metabolites. Mass spectrometry-based metabolomics analysis is the method of choice for the analysis of known and discovery of unknown metabolites. Advanced Chemical Biology tools are still limited in metabolomics compared to other ‘omics research fields. We have developed new state-of-the-art Chemical Biology methodologies for an enhanced metabolomics analysis using liquid chromatography-coupled with tandem mass spectrometry (UPLC-MS/MS). [1-7] These unique tools are aimed at overcoming limitations in mass spectrometry-based metabolomics analysis and are selective for microbiome metabolism. We are applying these methods for the analysis of human samples collected from pancreatic cancer patients. We have designed and synthesized a unique chemoselective probe immobilized to magnetic beads that allows for facile extraction of metabolites and led to increased mass spectrometric sensitivity by a factor of up to one million. [1-5] An incorporated bioorthogonal cleavage site, which we have adapted from a protecting group that is labile under mild, palladium-catalyzed conditions facilitates efficient release of captured metabolite without altering their chemical structure. This method was utilized on human fecal and isolated microbiome samples. Our analysis of carbonyls, thiols, amines, and short-chain fatty acids (SCFAs) revealed previously unknown metabolites and due to conjugation of the mass-spectrometric tag and separation from the sample background the detection limit was at high attomole quantities. We also utilized selective enzymatic treatment of metabolites in human samples
{"title":"Exploring Gut Microbiota Metabolism—New Chemical Biology Tools for Metabolomics Analysis","authors":"Vladyslav Dovhalyuk, Amanpreet Kaur, Weifeng Lin, Ioanna Tsiara, Sydney Mwasambu, Daniel Globisch","doi":"10.3390/proceedings2023092064","DOIUrl":"https://doi.org/10.3390/proceedings2023092064","url":null,"abstract":"One of the most exciting scientific developments in the past decade has been the realization that gut microbiota profoundly impact human physiology. The complex consortium of trillions of microbes possesses a wide range of metabolic activity. Due to the link to disease development from metagenomic analysis the targeted investigation of their metabolism represents a tremendous potential for the discovery of biomarkers and bioactive metabolites. Mass spectrometry-based metabolomics analysis is the method of choice for the analysis of known and discovery of unknown metabolites. Advanced Chemical Biology tools are still limited in metabolomics compared to other ‘omics research fields. We have developed new state-of-the-art Chemical Biology methodologies for an enhanced metabolomics analysis using liquid chromatography-coupled with tandem mass spectrometry (UPLC-MS/MS). [1-7] These unique tools are aimed at overcoming limitations in mass spectrometry-based metabolomics analysis and are selective for microbiome metabolism. We are applying these methods for the analysis of human samples collected from pancreatic cancer patients. We have designed and synthesized a unique chemoselective probe immobilized to magnetic beads that allows for facile extraction of metabolites and led to increased mass spectrometric sensitivity by a factor of up to one million. [1-5] An incorporated bioorthogonal cleavage site, which we have adapted from a protecting group that is labile under mild, palladium-catalyzed conditions facilitates efficient release of captured metabolite without altering their chemical structure. This method was utilized on human fecal and isolated microbiome samples. Our analysis of carbonyls, thiols, amines, and short-chain fatty acids (SCFAs) revealed previously unknown metabolites and due to conjugation of the mass-spectrometric tag and separation from the sample background the detection limit was at high attomole quantities. We also utilized selective enzymatic treatment of metabolites in human samples","PeriodicalId":504549,"journal":{"name":"International Conference EcoBalt 2023 \"Chemicals & Environment\"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139210780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：