Pub Date : 2024-04-20DOI: 10.15294/biosaintifika.v15i1.3503
Anang Kadarsah, Aminuddin Prahatama Putra, Anni Nurliani, Eko Suhartono, Sayed Ibrahim
Exploration of Heterotrigona itama biology characteristics besides being valuable for health and biodiversity also beneficial as well as an environmental bioindicator. This research aims to evaluate morphology characteristics and nest structure of stingless bee Heterotrigona itama from three different meliponiculture practices (MP1:meliponiculture in the back yard; MP2:meliponiculture in the rubber garden and MP3: meliponiculture in the side yard) in Banjar Regency, Indonesia. The parameters studied are morphology, nest design, colony structure, and environmental suitability. One-way ANOVA at a 95% confidence level was used to analyze differences between parameters. The results show Heterotrigona itama body size average from rubber gardens is bigger (7.11±0.60 mm) than the side yard (6.79±0.34 mm) and back yard (6.64±0.46 mm). The degree of difference is visible in the fore leg (α=0.00<0.05) and hind leg (α=0.004<0.05). The nest structure in MP3 was significantly different than MP1 and MP2, especially in terms of funnel length (p=0.007<0.05) and nest height from the ground (p=0.000<0.05). Environmental conditions MP3 (temperature 31.69±1.93 0C, humidity 60.70±11.2 % and water source) is more supportive for meliponiculture than MP2 (disturbed by household waste) and MP3 polluted by chicken farms. Meliponicultures practices based on various locations and environmental characteristics influence several parameters of Heterotrigona itama morphology and nest structure.
{"title":"Morphological Characteristics and Nest Structure of Stingless Bee (Heterotrigona itama) from Different Meliponiculture Practices","authors":"Anang Kadarsah, Aminuddin Prahatama Putra, Anni Nurliani, Eko Suhartono, Sayed Ibrahim","doi":"10.15294/biosaintifika.v15i1.3503","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i1.3503","url":null,"abstract":"Exploration of Heterotrigona itama biology characteristics besides being valuable for health and biodiversity also beneficial as well as an environmental bioindicator. This research aims to evaluate morphology characteristics and nest structure of stingless bee Heterotrigona itama from three different meliponiculture practices (MP1:meliponiculture in the back yard; MP2:meliponiculture in the rubber garden and MP3: meliponiculture in the side yard) in Banjar Regency, Indonesia. The parameters studied are morphology, nest design, colony structure, and environmental suitability. One-way ANOVA at a 95% confidence level was used to analyze differences between parameters. The results show Heterotrigona itama body size average from rubber gardens is bigger (7.11±0.60 mm) than the side yard (6.79±0.34 mm) and back yard (6.64±0.46 mm). The degree of difference is visible in the fore leg (α=0.00<0.05) and hind leg (α=0.004<0.05). The nest structure in MP3 was significantly different than MP1 and MP2, especially in terms of funnel length (p=0.007<0.05) and nest height from the ground (p=0.000<0.05). Environmental conditions MP3 (temperature 31.69±1.93 0C, humidity 60.70±11.2 % and water source) is more supportive for meliponiculture than MP2 (disturbed by household waste) and MP3 polluted by chicken farms. Meliponicultures practices based on various locations and environmental characteristics influence several parameters of Heterotrigona itama morphology and nest structure.","PeriodicalId":505566,"journal":{"name":"Biosaintifika: Journal of Biology & Biology Education","volume":"117 23","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140679134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-20DOI: 10.15294/biosaintifika.v15i1.3501
N. K. T. Martuti, Dwi Fiska Falisah, W. A. B. N. Sidiq, I. Melati, D. Mutiatari, Nasir Nayam
The coast of Tanjung Mas, Semarang City, is an industrial area used by the community to cultivate milkfish using stick-net pens. Furthermore, heavy metal contamination such as Cr, Cd, Cu, and Pb potentially disrupts milkfish meat's growth, quality, and safety. This study aims to determine heavy metals concentration in the waters and milkfish meat in stick-net pens cultivation in Tanjung Mas, Semarang City. The study was conducted using exploration, with five observation sites representing household waste disposal flow, industrial discharge, open sea, and intermediate areas. The observation sites were selected based on the milkfish cultivation activity. The sample and data, including water, milkfish, and environmental factors (temperature, pH, salinity, water current, and dissolved oxygen), were collected three times every two weeks. The heavy metals were detected using Atomic Absorption Spectrometry (AAS). The highest heavy metal concentration is Cr, which ranges from 1.70 ± 0.32 ppm to 2.36 ± 0.63 ppm in milkfish and 2.77 ± 0.65 ppm to 3.05 ± 0.58 ppm in the environment. The heavy metals contamination in Semarang City’s water areas is still relatively low and has no impact on milkfish growth. Industrial waste, mainly heavy metals, potentially threatens the stick-net pens cultivation model developed by the community in Semarang City. This study can be used as an input for mitigation and adaptive action in anticipating future environmental changes and maintaining their business sustainability.
{"title":"Bioconcentration of Heavy Metals in Milkfish Reared in Stick-Net Pens System: Implications for Open Water Environmental Contamination and Food Safety","authors":"N. K. T. Martuti, Dwi Fiska Falisah, W. A. B. N. Sidiq, I. Melati, D. Mutiatari, Nasir Nayam","doi":"10.15294/biosaintifika.v15i1.3501","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i1.3501","url":null,"abstract":"The coast of Tanjung Mas, Semarang City, is an industrial area used by the community to cultivate milkfish using stick-net pens. Furthermore, heavy metal contamination such as Cr, Cd, Cu, and Pb potentially disrupts milkfish meat's growth, quality, and safety. This study aims to determine heavy metals concentration in the waters and milkfish meat in stick-net pens cultivation in Tanjung Mas, Semarang City. The study was conducted using exploration, with five observation sites representing household waste disposal flow, industrial discharge, open sea, and intermediate areas. The observation sites were selected based on the milkfish cultivation activity. The sample and data, including water, milkfish, and environmental factors (temperature, pH, salinity, water current, and dissolved oxygen), were collected three times every two weeks. The heavy metals were detected using Atomic Absorption Spectrometry (AAS). The highest heavy metal concentration is Cr, which ranges from 1.70 ± 0.32 ppm to 2.36 ± 0.63 ppm in milkfish and 2.77 ± 0.65 ppm to 3.05 ± 0.58 ppm in the environment. The heavy metals contamination in Semarang City’s water areas is still relatively low and has no impact on milkfish growth. Industrial waste, mainly heavy metals, potentially threatens the stick-net pens cultivation model developed by the community in Semarang City. This study can be used as an input for mitigation and adaptive action in anticipating future environmental changes and maintaining their business sustainability.","PeriodicalId":505566,"journal":{"name":"Biosaintifika: Journal of Biology & Biology Education","volume":"117 20","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140680486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-20DOI: 10.15294/biosaintifika.v15i1.3519
Khoerina Salwa, R. Susanti, D. Utomo, Ari Yuniastuti, Nugrahaningsih Wh
Dengue virus infection causes thrombocytopenia. Psidium guajava is widely used by people to increase platelet counts. This research aims to analyze in silico the molecular mechanisms of compounds in guava fruit in increasing platelets. The compounds in guava fruit were taken from Dr. Duke's Phytochemical and Ethnobotanical Databases, which include secondary metabolites such as flavonoids, terpenoids, tannins, alkaloids, and fatty acids. Target proteins were predicted using PharmMapper. Protein interaction networks were created using STRING, visualized, and analyzed using Cytoscape. Potential target proteins were identified by topology, modularity, functional, and KEGG pathway analysis. Degree and betweenness centrality are parameters in topological analysis and the cluster with the highest score is selected as the functional module. The results showed that MAPK1, MAPK14, and AKT1 are involved in many inflammatory pathways, and MMP9 is a target protein directly involved in increasing vascular permeability. The compounds arjunolic acid, farnesene, beta-carotene, and alpha-linolenic acid inhibit MAPK1, citral, ellagic acid, palmitic acid, and oleanolic acid inhibit MAPK14, guaijaverin, pantothenic acid, and citric acid inhibit AKT1, guaijaverin and pantothenic acid inhibit MMP9. It was concluded that the bioactive compounds in guava fruit play a role in increasing platelets by inhibiting the MAPK, PI3K-Akt pathways, and leukocyte trans-endothelial migration, thereby inhibiting or reducing the production and expression of inflammatory mediators and vascular permeability.
{"title":"Molecular Mechanisms of Increased Platelets: An In Silico of the Active Compounds in Psidium guajava","authors":"Khoerina Salwa, R. Susanti, D. Utomo, Ari Yuniastuti, Nugrahaningsih Wh","doi":"10.15294/biosaintifika.v15i1.3519","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i1.3519","url":null,"abstract":"Dengue virus infection causes thrombocytopenia. Psidium guajava is widely used by people to increase platelet counts. This research aims to analyze in silico the molecular mechanisms of compounds in guava fruit in increasing platelets. The compounds in guava fruit were taken from Dr. Duke's Phytochemical and Ethnobotanical Databases, which include secondary metabolites such as flavonoids, terpenoids, tannins, alkaloids, and fatty acids. Target proteins were predicted using PharmMapper. Protein interaction networks were created using STRING, visualized, and analyzed using Cytoscape. Potential target proteins were identified by topology, modularity, functional, and KEGG pathway analysis. Degree and betweenness centrality are parameters in topological analysis and the cluster with the highest score is selected as the functional module. The results showed that MAPK1, MAPK14, and AKT1 are involved in many inflammatory pathways, and MMP9 is a target protein directly involved in increasing vascular permeability. The compounds arjunolic acid, farnesene, beta-carotene, and alpha-linolenic acid inhibit MAPK1, citral, ellagic acid, palmitic acid, and oleanolic acid inhibit MAPK14, guaijaverin, pantothenic acid, and citric acid inhibit AKT1, guaijaverin and pantothenic acid inhibit MMP9. It was concluded that the bioactive compounds in guava fruit play a role in increasing platelets by inhibiting the MAPK, PI3K-Akt pathways, and leukocyte trans-endothelial migration, thereby inhibiting or reducing the production and expression of inflammatory mediators and vascular permeability.","PeriodicalId":505566,"journal":{"name":"Biosaintifika: Journal of Biology & Biology Education","volume":"106 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140679651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-16DOI: 10.15294/biosaintifika.v14i3.43299
Soimatun Aliyah, Arif Widiyatmoko
Courageous learning triggers learning loss, which is characterized by a decrease in the ability to think critically and creatively. The solution used with innovation in learning media is the e-model of entrepreneurship. This study aims to test the feasibility of entrepreneurship-based biotechnology e-modules and improve students' critical and creative thinking skills after using this e-module. This research and development (RD), which applies the 4D model (Define, Design, Develop, and Dissemination). The study consisted of 31 class IXA students of yunior high school. The research instrument consisted of expert validation questionnaires, pretest-posttest questions, and student response questionnaires. The analysis of the validation questionnaire for material and media experts produced an average score quite high with the appropriate criteria for use in classroom learning. The results of the t-test for students' critical and creative thinking skills yield a significant value of 0.0 which is smaller than 0.05, which means that there is a significant difference between students' critical and creative thinking skills in the pretest and post-test. Analysis of the N-gain value of students' critical and creative thinking abilities shows an average result of 0.46. This result is included in the category of moderate improvement, which means that the increase in students' critical and creative thinking skills is in the medium category. This e-module also has a very good response from all students. Based on the results of these analyses, the entrepreneurship-based biotechnology e-module is declared feasible for use in learning and can improve students' critical and creative thinking abilities.
{"title":"Entrepreneurship-Based Biotechnology E-Module Development to Improve Critical and Creative Thinking Skills","authors":"Soimatun Aliyah, Arif Widiyatmoko","doi":"10.15294/biosaintifika.v14i3.43299","DOIUrl":"https://doi.org/10.15294/biosaintifika.v14i3.43299","url":null,"abstract":"Courageous learning triggers learning loss, which is characterized by a decrease in the ability to think critically and creatively. The solution used with innovation in learning media is the e-model of entrepreneurship. This study aims to test the feasibility of entrepreneurship-based biotechnology e-modules and improve students' critical and creative thinking skills after using this e-module. This research and development (RD), which applies the 4D model (Define, Design, Develop, and Dissemination). The study consisted of 31 class IXA students of yunior high school. The research instrument consisted of expert validation questionnaires, pretest-posttest questions, and student response questionnaires. The analysis of the validation questionnaire for material and media experts produced an average score quite high with the appropriate criteria for use in classroom learning. The results of the t-test for students' critical and creative thinking skills yield a significant value of 0.0 which is smaller than 0.05, which means that there is a significant difference between students' critical and creative thinking skills in the pretest and post-test. Analysis of the N-gain value of students' critical and creative thinking abilities shows an average result of 0.46. This result is included in the category of moderate improvement, which means that the increase in students' critical and creative thinking skills is in the medium category. This e-module also has a very good response from all students. Based on the results of these analyses, the entrepreneurship-based biotechnology e-module is declared feasible for use in learning and can improve students' critical and creative thinking abilities.","PeriodicalId":505566,"journal":{"name":"Biosaintifika: Journal of Biology & Biology Education","volume":"87 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139176811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-15DOI: 10.15294/biosaintifika.v14i3.42919
Harmita Aprilanti, E. S. Rahayu, L. N. Hadiyanti
Authentic learning resources are meaningful for Biology learning, particularly in conceiving Plantae topic. The availability of plants, especially Pteridophyte in school environment is very limited and require more adequate plant learning resources. This study aimed to develop a Pteridophytes Taxonomic Learning Resource (PTLR) and tested its validity, practicality, and effectiveness. Research and Development were done refers to the ADDIE model. The PTLR was validated by a taxonomist and instructional media expert. The practicality was observed from the responses of teachers and students, while the effectiveness was explored based on cognitive and psychomotor learning outcomes. The PTLR was developed by container gardening method with 29 fern species representing the classes of Pteridophyte and showed specific characteristics which organized in their taxonomic groups. The results showed that PTLR is very valid with average score of 85.5% and considered practical by teachers (81.25%) and students (90.65%). The PTLR was also effective in achieving two basic competencies by Curriculum of 2013 of Biology with cognitive outcome N-gain of 0.57 (moderate) and psychomotor 86.34% (very good). In summing up, the PTLR is recommended as a learning resource of Plantae topic at senior high school. This research result enriches the types of innovative biology learning resources.
{"title":"Development of Pteridophyte Taxonomic Learning Resource to Foster Cognitive and Psychomotor Learning Outcomes","authors":"Harmita Aprilanti, E. S. Rahayu, L. N. Hadiyanti","doi":"10.15294/biosaintifika.v14i3.42919","DOIUrl":"https://doi.org/10.15294/biosaintifika.v14i3.42919","url":null,"abstract":"Authentic learning resources are meaningful for Biology learning, particularly in conceiving Plantae topic. The availability of plants, especially Pteridophyte in school environment is very limited and require more adequate plant learning resources. This study aimed to develop a Pteridophytes Taxonomic Learning Resource (PTLR) and tested its validity, practicality, and effectiveness. Research and Development were done refers to the ADDIE model. The PTLR was validated by a taxonomist and instructional media expert. The practicality was observed from the responses of teachers and students, while the effectiveness was explored based on cognitive and psychomotor learning outcomes. The PTLR was developed by container gardening method with 29 fern species representing the classes of Pteridophyte and showed specific characteristics which organized in their taxonomic groups. The results showed that PTLR is very valid with average score of 85.5% and considered practical by teachers (81.25%) and students (90.65%). The PTLR was also effective in achieving two basic competencies by Curriculum of 2013 of Biology with cognitive outcome N-gain of 0.57 (moderate) and psychomotor 86.34% (very good). In summing up, the PTLR is recommended as a learning resource of Plantae topic at senior high school. This research result enriches the types of innovative biology learning resources.","PeriodicalId":505566,"journal":{"name":"Biosaintifika: Journal of Biology & Biology Education","volume":"113 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139178515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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