Neonate lung injury is a common phenomenon after perinatal asphyxia To evaluate proteomic profiles of exosomes isolated from lung injury offspring serum after perinatal asphyxia. Serum samples were collected at 12 h, 24 h, and 72 h after birth in neonates with perinatal asphyxia-induced lung injury. Exosomes were isolated, and the concentration and size distribution were assessed. The exosome surface markers CD9, CD63, CD81, HSP70, and TSG101 were detected by Western blot. The exosome proteins were evaluated by quantitative proteomics using a tandem mass tag (TMT). All the identified proteins were submitted to the Weighted Gene Co-Expression Network Analysis (WGCNA), GO function, and KEGG pathway analysis. A protein-protein interaction network (PPI) was utilized to identify hub proteins with the Cytohubba plugin of Cytoscape. The exosomes were round or oval vesicular structures at a diameter range of 100-200 nm, and the size distribution was standard and consistent. Exosome surface markers CD9, CD63, CD81, HSP70, and TSG101 were detected. 444 out of 450 proteins were mapped with gene names. A brown module containing 71 proteins was highly linked with the 12 h phenotype and was predominantly concentrated in lipoprotein and complement activation. The top 10 proteins, APOA1, APOB, APOE, LPA, APOA2, CP, C3, FGB, FGA, and TF, were determined as hub proteins. The present study demonstrates comprehensive information for understanding molecular changes of lung injury following perinatal asphyxia, which provides a reliable basis for screening potential biomarkers and therapeutic targets in the clinic.
{"title":"Proteome Profiling of Serum Exosomes from Newborns with Lung Injury after Perinatal Asphyxia","authors":"Haiying Li, Chuangli Hao, Feifei Shen, Ying Li, W. Gu, Xingmei Yu, Youjia Wu, Gui-hai Suo, Yu-qin Zheng","doi":"10.2174/1570164620666230714115822","DOIUrl":"https://doi.org/10.2174/1570164620666230714115822","url":null,"abstract":"\u0000\u0000Neonate lung injury is a common phenomenon after perinatal asphyxia\u0000\u0000\u0000\u0000To evaluate proteomic profiles of exosomes isolated from lung injury offspring serum after perinatal asphyxia.\u0000\u0000\u0000\u0000Serum samples were collected at 12 h, 24 h, and 72 h after birth in neonates with perinatal asphyxia-induced lung injury. Exosomes were isolated, and the concentration and size distribution were assessed. The exosome surface markers CD9, CD63, CD81, HSP70, and TSG101 were detected by Western blot. The exosome proteins were evaluated by quantitative proteomics using a tandem mass tag (TMT). All the identified proteins were submitted to the Weighted Gene Co-Expression Network Analysis (WGCNA), GO function, and KEGG pathway analysis. A protein-protein interaction network (PPI) was utilized to identify hub proteins with the Cytohubba plugin of Cytoscape.\u0000\u0000\u0000\u0000The exosomes were round or oval vesicular structures at a diameter range of 100-200 nm, and the size distribution was standard and consistent. Exosome surface markers CD9, CD63, CD81, HSP70, and TSG101 were detected. 444 out of 450 proteins were mapped with gene names. A brown module containing 71 proteins was highly linked with the 12 h phenotype and was predominantly concentrated in lipoprotein and complement activation. The top 10 proteins, APOA1, APOB, APOE, LPA, APOA2, CP, C3, FGB, FGA, and TF, were determined as hub proteins.\u0000\u0000\u0000\u0000The present study demonstrates comprehensive information for understanding molecular changes of lung injury following perinatal asphyxia, which provides a reliable basis for screening potential biomarkers and therapeutic targets in the clinic.\u0000","PeriodicalId":50601,"journal":{"name":"Current Proteomics","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72521095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-08DOI: 10.2174/1570164620666230508140912
Ajay Vir Singh, Shweta Kushwaha, R. Yadav, Kusuma Sai Davuluri, A. Goel, Devendra Singh Chauhan
Despite the crucial involvement of vitronectin in affecting the perseverance of certain respiratory pathogens and the progression of several lung diseases, the association of vitronectin with tuberculosis (TB) has been poorly studied. The present study aimed to determine whether vitronectin levels are altered in TB patients compared to healthy humans. Twenty-four laboratory-confirmed tuberculosis patients (pulmonary TB -8, extrapulmonary-8 and HIV-TB dual infected -8) and eight healthy individuals were included in this study. The quantitative detection of vitronectin in serum-derived exosomes of study participants was performed using a sandwich enzyme-linked immunosorbent assay. Measured concentrations of vitronectin were compared with the demographic variables of the study participants and between the study groups. The Mann–Whitney U unpaired test was used in statistical analysis, and the p-value < 0.05 was considered statistically significant. Vitronectin was detected in serum-derived exosomes of all study participants. The demographic characteristics (gender, age, smoking and alcohol consumption habit, history of cough, and weight loss) were not significantly correlated with the vitronectin concentrations of the study participants (p-value> 0.05). The level of vitronectin was higher in patients with pulmonary TB (778.54 ng/l) and extra-pulmonary-TB patients (773.04 ng/l) while lower in HIV-pulmonary TB dual-infected patients (354.86 ng/l) as compared to healthy humans (456.20ng/l). There was a significant difference between vitronectin concentrations of patients with pulmonary TB (p-value: 0.0002) and extrapulmonary TB (p-value: 0.003) compared to healthy controls. The present study reported an increased concentration of vitronectin in serum-derived exosomes of pulmonary and extra-pulmonary TB patients compared to HIV-TB dual-infected patients and healthy humans. Further studies are needed to fully elucidate the diagnostic potential and functionalities of higher concentrations of vitronectin in the pathogenic processes of human TB.
尽管玻璃体粘连蛋白在影响某些呼吸道病原体的持久性和几种肺部疾病的进展中起着至关重要的作用,但玻璃体粘连蛋白与结核病(TB)的关系研究甚少。目前的研究旨在确定结核患者的玻璃体粘连蛋白水平是否与健康人相比有所改变。本研究纳入24例实验室确诊结核病患者(肺结核-8、肺外-8和HIV-TB双重感染-8)和8例健康个体。使用三明治酶联免疫吸附法定量检测研究参与者血清衍生外泌体中的玻璃体粘连蛋白。将检测到的玻璃体粘连素浓度与研究参与者的人口学变量以及研究组之间的变量进行比较。统计学分析采用Mann-Whitney U unpaired检验,p值< 0.05认为有统计学意义。在所有研究参与者的血清衍生外泌体中检测到玻璃体连接蛋白。人口统计学特征(性别、年龄、吸烟和饮酒习惯、咳嗽史和体重减轻)与研究参与者的玻璃体粘连素浓度无显著相关性(p值> 0.05)。肺结核患者(778.54 ng/l)和肺外结核患者(773.04 ng/l)的玻璃连接蛋白水平较高,而hiv -肺结核双感染患者(354.86 ng/l)的玻璃连接蛋白水平低于健康人(456.20ng/l)。肺结核患者的玻璃体粘连蛋白浓度(p值:0.0002)和肺外结核患者的玻璃体粘连蛋白浓度(p值:0.003)与健康对照有显著差异。本研究报道,与HIV-TB双重感染患者和健康人相比,肺部和肺外结核患者血清衍生黏着体中的玻璃体粘连蛋白浓度增加。需要进一步的研究来充分阐明较高浓度的玻璃体粘连蛋白在人结核发病过程中的诊断潜力和功能。
{"title":"Higher Abundance of Vitronectin (S-protein) in Serum-derived Exosomes\u0000of Pulmonary and Extra-Pulmonary Tuberculosis Patients as Compared\u0000to HIV-Tuberculosis Dual-infected Patients and Healthy Humans","authors":"Ajay Vir Singh, Shweta Kushwaha, R. Yadav, Kusuma Sai Davuluri, A. Goel, Devendra Singh Chauhan","doi":"10.2174/1570164620666230508140912","DOIUrl":"https://doi.org/10.2174/1570164620666230508140912","url":null,"abstract":"\u0000\u0000Despite the crucial involvement of vitronectin in affecting the perseverance\u0000of certain respiratory pathogens and the progression of several lung diseases, the association of vitronectin with tuberculosis (TB) has been poorly studied. The present study aimed to determine whether\u0000vitronectin levels are altered in TB patients compared to healthy humans.\u0000\u0000\u0000\u0000Twenty-four laboratory-confirmed tuberculosis patients (pulmonary TB -8, extrapulmonary-8 and HIV-TB dual infected -8) and eight healthy individuals were included in this study.\u0000The quantitative detection of vitronectin in serum-derived exosomes of study participants was performed using a sandwich enzyme-linked immunosorbent assay. Measured concentrations of vitronectin were compared with the demographic variables of the study participants and between the study\u0000groups. The Mann–Whitney U unpaired test was used in statistical analysis, and the p-value < 0.05\u0000was considered statistically significant.\u0000\u0000\u0000\u0000Vitronectin was detected in serum-derived exosomes of all study participants. The demographic characteristics (gender, age, smoking and alcohol consumption habit, history of cough, and\u0000weight loss) were not significantly correlated with the vitronectin concentrations of the study participants (p-value> 0.05). The level of vitronectin was higher in patients with pulmonary TB (778.54 ng/l)\u0000and extra-pulmonary-TB patients (773.04 ng/l) while lower in HIV-pulmonary TB dual-infected patients (354.86 ng/l) as compared to healthy humans (456.20ng/l). There was a significant difference\u0000between vitronectin concentrations of patients with pulmonary TB (p-value: 0.0002) and extrapulmonary TB (p-value: 0.003) compared to healthy controls.\u0000\u0000\u0000\u0000The present study reported an increased concentration of vitronectin in serum-derived\u0000exosomes of pulmonary and extra-pulmonary TB patients compared to HIV-TB dual-infected patients\u0000and healthy humans. Further studies are needed to fully elucidate the diagnostic potential and functionalities of higher concentrations of vitronectin in the pathogenic processes of human TB.\u0000","PeriodicalId":50601,"journal":{"name":"Current Proteomics","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90681594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}