ABSTRACT Here, we report the distribution of HLA-DPB1 alleles studied in a cohort of 55 randomly collected blood samples from unrelated Taiwanese individuals and the deduced most likely HLA haplotypes associated with the defined DPB1 alleles in the cohort. Our aim is to reveal the unprecedented data on the distribution of HLA-DPB1 alleles in the Taiwanese population and to find out the most probable HLA haplotypes associated with the HLA-DPB1 alleles detected. The material for this study was blood samples, preserved in K2EDTA and/or acid citrate dextrose anticoagulants. The blood donors were voluntary individuals of Tzu Chi Bone Marrow Donor Registry, Tzu Chi Stem Cells Center, Hualien Tzu Chi Hospital. Sequence-based typing of the Sanger’s sequencing method was performed for the HLA allelic typing. To discern the HLA-DPB1 alleles, exons 2 and 3 of the HLA-DPB1 locus were sequenced. Target exon sequence amplifications were achieved by polymerase chain reaction, and the resulting amplicons were sequenced by BigDye Terminator Cycle Sequencing Ready Reaction Kit according to the manufacturer’s protocols. In the total number of 55 randomized unrelated Taiwanese individuals studied, we detected 11 different HLA-DPB1 alleles. DPB1*05:01 (44.54%) was the allele with the highest frequency observed and the next highest frequency allele found was DPB1*02:01 (17.27%), while DPB1*38:01 (0.90%) and DPB1*700:01N (0.90%) ranked the least observed DPB1 alleles. Our findings in this study may be useful in researches reinforcing the comprehensive understanding on the distribution of DPB1 alleles and their associated HLA haplotypes and their clinical applications in Taiwanese.
{"title":"Recognition of HLA-DPB1 alleles and their associated HLA haplotypes in 55 randomized unrelated Taiwanese individuals","authors":"Shee-Ping Chen, P. Lin, Kuo‐Liang Yang","doi":"10.4103/tcmj.tcmj_68_24","DOIUrl":"https://doi.org/10.4103/tcmj.tcmj_68_24","url":null,"abstract":"ABSTRACT\u0000 \u0000 \u0000 \u0000 Here, we report the distribution of HLA-DPB1 alleles studied in a cohort of 55 randomly collected blood samples from unrelated Taiwanese individuals and the deduced most likely HLA haplotypes associated with the defined DPB1 alleles in the cohort. Our aim is to reveal the unprecedented data on the distribution of HLA-DPB1 alleles in the Taiwanese population and to find out the most probable HLA haplotypes associated with the HLA-DPB1 alleles detected.\u0000 \u0000 \u0000 \u0000 The material for this study was blood samples, preserved in K2EDTA and/or acid citrate dextrose anticoagulants. The blood donors were voluntary individuals of Tzu Chi Bone Marrow Donor Registry, Tzu Chi Stem Cells Center, Hualien Tzu Chi Hospital. Sequence-based typing of the Sanger’s sequencing method was performed for the HLA allelic typing. To discern the HLA-DPB1 alleles, exons 2 and 3 of the HLA-DPB1 locus were sequenced. Target exon sequence amplifications were achieved by polymerase chain reaction, and the resulting amplicons were sequenced by BigDye Terminator Cycle Sequencing Ready Reaction Kit according to the manufacturer’s protocols.\u0000 \u0000 \u0000 \u0000 In the total number of 55 randomized unrelated Taiwanese individuals studied, we detected 11 different HLA-DPB1 alleles. DPB1*05:01 (44.54%) was the allele with the highest frequency observed and the next highest frequency allele found was DPB1*02:01 (17.27%), while DPB1*38:01 (0.90%) and DPB1*700:01N (0.90%) ranked the least observed DPB1 alleles.\u0000 \u0000 \u0000 \u0000 Our findings in this study may be useful in researches reinforcing the comprehensive understanding on the distribution of DPB1 alleles and their associated HLA haplotypes and their clinical applications in Taiwanese.\u0000","PeriodicalId":507485,"journal":{"name":"Tzu Chi Medical Journal","volume":" 77","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141827030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT Guo Min decoction (GMD) is a Chinese traditional medicine that can regulate allergy-related symptoms. Although GMD treatment was reported to treat allergy-associated symptoms by regulating the immune response, the rationale between GMD treatment and angiogenesis has not been reported yet. Our objective is to investigate the angiogenesis-modulating activity of GMD. In this study, we used fluorescence recording, alkaline phosphatase (AP) activity staining, and real-time polymerase chain reaction (PCR) experiments to examine the effects of GMD on angiogenesis in a zebrafish model. GMD-treated zebrafish embryos exhibited more intercapillary spaces in the caudal vein plexus (Mock: 11.1 ± 1.8 [n = 20; n: numbers of embryos]; GMD-treated: 16.2 ± 1.9 [n = 20]). AP activity staining showed that treatment with GMD and liquorice (Gan Cao, a component of GMD) induced subintestinal vein outgrowth. However, glycyrrhizin (a component of Gan Cao) had no obvious pro-angiogenic effects on zebrafish. Furthermore, real-time PCR experiments indicated that GMD exposure might be through regulating angiogenesis-related genes (cdh5, nrp1a, and flt1) expressions. Based on these observations, we proposed that GMD had pro-angiogenic activity in a zebrafish model, and it might partially be contributed by one of the components, liquorice.
{"title":"Pro-angiogenic effects of Guo Min decoction in a zebrafish model","authors":"Ping-Hsun Lu, S. Tung, Chi-Chung Wen, Ching-Yuan Huang, Jhih-Wei Huang, Chih-Hsin Chen, Yau-Hung Chen","doi":"10.4103/tcmj.tcmj_59_24","DOIUrl":"https://doi.org/10.4103/tcmj.tcmj_59_24","url":null,"abstract":"ABSTRACT\u0000 \u0000 \u0000 \u0000 Guo Min decoction (GMD) is a Chinese traditional medicine that can regulate allergy-related symptoms. Although GMD treatment was reported to treat allergy-associated symptoms by regulating the immune response, the rationale between GMD treatment and angiogenesis has not been reported yet. Our objective is to investigate the angiogenesis-modulating activity of GMD.\u0000 \u0000 \u0000 \u0000 In this study, we used fluorescence recording, alkaline phosphatase (AP) activity staining, and real-time polymerase chain reaction (PCR) experiments to examine the effects of GMD on angiogenesis in a zebrafish model.\u0000 \u0000 \u0000 \u0000 GMD-treated zebrafish embryos exhibited more intercapillary spaces in the caudal vein plexus (Mock: 11.1 ± 1.8 [n = 20; n: numbers of embryos]; GMD-treated: 16.2 ± 1.9 [n = 20]). AP activity staining showed that treatment with GMD and liquorice (Gan Cao, a component of GMD) induced subintestinal vein outgrowth. However, glycyrrhizin (a component of Gan Cao) had no obvious pro-angiogenic effects on zebrafish. Furthermore, real-time PCR experiments indicated that GMD exposure might be through regulating angiogenesis-related genes (cdh5, nrp1a, and flt1) expressions.\u0000 \u0000 \u0000 \u0000 Based on these observations, we proposed that GMD had pro-angiogenic activity in a zebrafish model, and it might partially be contributed by one of the components, liquorice.\u0000","PeriodicalId":507485,"journal":{"name":"Tzu Chi Medical Journal","volume":"37 30","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141645090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT Laparoscopic hepatectomy (LH) for hepatocellular carcinoma (HCC) has been well known for its advantages in the past 10 years, but little is known regarding its oncologic outcomes while the technique is being developed at an institution. This study aimed to evaluate the safety and effectiveness of LH for patients with primary HCC at favorable locations, focusing on postoperative short- and long-term outcomes during the development period. We retrospectively reviewed patients diagnosed with primary HCC who underwent hepatectomy between January 2013 and December 2019 at Hualien Tzu Chi Hospital. Patients with HCC at favorable locations (anterolateral segments) were collected and divided into laparoscopic and open hepatectomy (OH) groups. The data for long-term outcomes, as the primary endpoint, and postoperative outcomes, as the secondary endpoint, were collected. The review included 159 patients, among which 42 and 44 patients in favorable locations underwent open and laparoscopic hepatectomies, respectively. There were no significant differences in intraoperative blood loss, major complication rate, and 90-day mortality rate between the two groups. The laparoscopic group had a lower transfusion rate, shorter postoperative hospital stay, and lower 90-day readmission rate. There were no significant differences in 12-, 36-, and 60-month overall survival and disease-free survival. LH for favorably located HCC is the preferred surgical approach compared to OH due to the decreased transfusion rate, shorter postoperative hospital stay, and lower 90-day readmission rate. LH did not compromise the 90-day mortality rate with sustained long-term overall and disease-free survival. LH for favorably located HCC is a safe and effective surgical approach even during the development period.
{"title":"Laparoscopic hepatectomy is a feasible and safe choice for primary hepatocellular carcinoma located at favorable location during the development period of a tertiary hospital: A case–control study","authors":"Yi-Hsuan Lee, Yu-Ting Huang, Tsai-Ling Kuo, Ming-Che Lee, Yen-Cheng Chen","doi":"10.4103/tcmj.tcmj_5_24","DOIUrl":"https://doi.org/10.4103/tcmj.tcmj_5_24","url":null,"abstract":"ABSTRACT\u0000 \u0000 \u0000 \u0000 Laparoscopic hepatectomy (LH) for hepatocellular carcinoma (HCC) has been well known for its advantages in the past 10 years, but little is known regarding its oncologic outcomes while the technique is being developed at an institution. This study aimed to evaluate the safety and effectiveness of LH for patients with primary HCC at favorable locations, focusing on postoperative short- and long-term outcomes during the development period.\u0000 \u0000 \u0000 \u0000 We retrospectively reviewed patients diagnosed with primary HCC who underwent hepatectomy between January 2013 and December 2019 at Hualien Tzu Chi Hospital. Patients with HCC at favorable locations (anterolateral segments) were collected and divided into laparoscopic and open hepatectomy (OH) groups. The data for long-term outcomes, as the primary endpoint, and postoperative outcomes, as the secondary endpoint, were collected.\u0000 \u0000 \u0000 \u0000 The review included 159 patients, among which 42 and 44 patients in favorable locations underwent open and laparoscopic hepatectomies, respectively. There were no significant differences in intraoperative blood loss, major complication rate, and 90-day mortality rate between the two groups. The laparoscopic group had a lower transfusion rate, shorter postoperative hospital stay, and lower 90-day readmission rate. There were no significant differences in 12-, 36-, and 60-month overall survival and disease-free survival.\u0000 \u0000 \u0000 \u0000 LH for favorably located HCC is the preferred surgical approach compared to OH due to the decreased transfusion rate, shorter postoperative hospital stay, and lower 90-day readmission rate. LH did not compromise the 90-day mortality rate with sustained long-term overall and disease-free survival. LH for favorably located HCC is a safe and effective surgical approach even during the development period.\u0000","PeriodicalId":507485,"journal":{"name":"Tzu Chi Medical Journal","volume":"113 49","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141666717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT Head-and-neck cancer is a major cancer in Taiwan. Most patients are in the advanced stage at initial diagnosis. In addition to primary surgery, adjuvant therapy, including chemotherapy and radiotherapy, is also necessary to treat these patients. We used a machine learning tool to determine the factors that may be associated with and predict treatment outcome. We retrospectively reviewed 187 patients diagnosed with advanced-stage head-and-neck cancer who received surgery and adjuvant radiotherapy with or without chemotherapy. We used eXtreme Gradient Boosting (XGBoost) – a gradient tree-based model – to analyze data. The features were extracted from the entries we recorded from the electronic health-care system and paper medical record. The patient data were categorized into training and testing datasets, with labeling according to their recurrence status within the 5-year follow-up. The primary endpoint was to predict whether the patients had recurrent disease. The risk factors were identified by analyzing the feature importance in the model. For comparison, we also used regression to perform the variate analysis to identify the risk factors. The accuracy, sensitivity, and positive predictive value of the model were 57.89%, 57.14%, and 44.44%, respectively. Pathological lymph node status was the most important feature, followed by whether the patient was receiving chemotherapy. Fraction size, early termination, and interruption were the important factors related to radiotherapy and might affect treatment outcome. The area under the curve of the receiver operating characteristic curve was 0.58. The risk factors identified by XGBoost were consistent with those found by regression. We found that several factors were associated with treatment outcome in advanced-stage head-and-neck cancer. In future, we hope to collect the data according to the features introduced in this study and to construct a stronger model to explain and predict outcomes.
{"title":"Using a machine learning algorithm and clinical data to predict the risk factors of disease recurrence after adjuvant treatment of advanced-stage oral cavity cancer","authors":"Sheng-Yao Huang, Ren-Jun Hsu, Dai-Wei Liu, Wen-Lin Hsu","doi":"10.4103/tcmj.tcmj_56_24","DOIUrl":"https://doi.org/10.4103/tcmj.tcmj_56_24","url":null,"abstract":"ABSTRACT\u0000 \u0000 \u0000 \u0000 Head-and-neck cancer is a major cancer in Taiwan. Most patients are in the advanced stage at initial diagnosis. In addition to primary surgery, adjuvant therapy, including chemotherapy and radiotherapy, is also necessary to treat these patients. We used a machine learning tool to determine the factors that may be associated with and predict treatment outcome.\u0000 \u0000 \u0000 \u0000 We retrospectively reviewed 187 patients diagnosed with advanced-stage head-and-neck cancer who received surgery and adjuvant radiotherapy with or without chemotherapy. We used eXtreme Gradient Boosting (XGBoost) – a gradient tree-based model – to analyze data. The features were extracted from the entries we recorded from the electronic health-care system and paper medical record. The patient data were categorized into training and testing datasets, with labeling according to their recurrence status within the 5-year follow-up. The primary endpoint was to predict whether the patients had recurrent disease. The risk factors were identified by analyzing the feature importance in the model. For comparison, we also used regression to perform the variate analysis to identify the risk factors.\u0000 \u0000 \u0000 \u0000 The accuracy, sensitivity, and positive predictive value of the model were 57.89%, 57.14%, and 44.44%, respectively. Pathological lymph node status was the most important feature, followed by whether the patient was receiving chemotherapy. Fraction size, early termination, and interruption were the important factors related to radiotherapy and might affect treatment outcome. The area under the curve of the receiver operating characteristic curve was 0.58. The risk factors identified by XGBoost were consistent with those found by regression.\u0000 \u0000 \u0000 \u0000 We found that several factors were associated with treatment outcome in advanced-stage head-and-neck cancer. In future, we hope to collect the data according to the features introduced in this study and to construct a stronger model to explain and predict outcomes.\u0000","PeriodicalId":507485,"journal":{"name":"Tzu Chi Medical Journal","volume":" January","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141670003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ping Chen, Baibai Ye, Cheng Lin, Chenning Zhang, Jia Chen, Linfu Li
ABSTRACT This study aimed to explore the potential mechanisms of TMF (5,7,3’,4’-tetramethoxyflavone) in treating osteoarthritis (OA) using network pharmacology and molecular docking. Databases including SwissTargetPrediction, BATMAN-TCM, PharmMapper, TargetNet, SuperPred, and SEA were utilized to screen the targets of TMF. “OA” was used as the disease keyword to predict OA-related genes through GeneCards, Therapeutic Target Database, PharmGKB, Online Mendelian Inheritance in Man, and Comparative Toxicogenomics Database. The Venn diagram was employed to identify the intersection of predicted targets between TMF and OA as potential targets for TMF in treating OA. The intersection targets were input into the STRING 12.0 online database to construct a protein–protein interaction (PPI) network and identify core targets. Subsequently, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed using the Metascape V3.5 online database platform. Finally, molecular docking between TMF and core targets was conducted using AutoDockTools 1.5.6. A total of 228 intersection targets for TMF treating OA were obtained, and PPI network analysis identified 5 core targets: STAT3, SRC, CTNNB1, EGFR, and AKT1. GO enrichment analysis yielded 2736 results, while KEGG analysis identified 203 pathways. Most elated GO and KEGG items of TMF in treating OA may include hormonal responses, antiviral and anticancer effects, anti-inflammation, phosphorus metabolism, phosphate metabolism, nitrogen compound responses, cancer-related pathways, PI3K-Akt signaling pathway, and MAPK signaling pathway. Molecular docking revealed good binding affinities between TMF and all core targets except STAT3. TMF might act on multiple targets and activate diverse pathways to intervene in OA, revealing the molecular processes involved in TMF treatment of OA.
{"title":"Exploration of the mechanism of tetramethoxyflavone in treating osteoarthritis based on network pharmacology and molecular docking","authors":"Ping Chen, Baibai Ye, Cheng Lin, Chenning Zhang, Jia Chen, Linfu Li","doi":"10.4103/tcmj.tcmj_77_24","DOIUrl":"https://doi.org/10.4103/tcmj.tcmj_77_24","url":null,"abstract":"ABSTRACT\u0000 \u0000 \u0000 \u0000 This study aimed to explore the potential mechanisms of TMF (5,7,3’,4’-tetramethoxyflavone) in treating osteoarthritis (OA) using network pharmacology and molecular docking.\u0000 \u0000 \u0000 \u0000 Databases including SwissTargetPrediction, BATMAN-TCM, PharmMapper, TargetNet, SuperPred, and SEA were utilized to screen the targets of TMF. “OA” was used as the disease keyword to predict OA-related genes through GeneCards, Therapeutic Target Database, PharmGKB, Online Mendelian Inheritance in Man, and Comparative Toxicogenomics Database. The Venn diagram was employed to identify the intersection of predicted targets between TMF and OA as potential targets for TMF in treating OA. The intersection targets were input into the STRING 12.0 online database to construct a protein–protein interaction (PPI) network and identify core targets. Subsequently, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed using the Metascape V3.5 online database platform. Finally, molecular docking between TMF and core targets was conducted using AutoDockTools 1.5.6.\u0000 \u0000 \u0000 \u0000 A total of 228 intersection targets for TMF treating OA were obtained, and PPI network analysis identified 5 core targets: STAT3, SRC, CTNNB1, EGFR, and AKT1. GO enrichment analysis yielded 2736 results, while KEGG analysis identified 203 pathways. Most elated GO and KEGG items of TMF in treating OA may include hormonal responses, antiviral and anticancer effects, anti-inflammation, phosphorus metabolism, phosphate metabolism, nitrogen compound responses, cancer-related pathways, PI3K-Akt signaling pathway, and MAPK signaling pathway. Molecular docking revealed good binding affinities between TMF and all core targets except STAT3.\u0000 \u0000 \u0000 \u0000 TMF might act on multiple targets and activate diverse pathways to intervene in OA, revealing the molecular processes involved in TMF treatment of OA.\u0000","PeriodicalId":507485,"journal":{"name":"Tzu Chi Medical Journal","volume":" 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141668519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-03DOI: 10.4103/tcmj.tcmj_253_23
Yu‐Ming Cheng, Shao-Wen Wang, Chia-Chi Wang, J. Kao
ABSTRACT Metabolic-associated fatty liver disease (MAFLD) was proposed in 2020 to replace the original term nonalcoholic fatty liver disease (NAFLD) with new diagnostic criteria. The disease risks of lean and overweight/obese MAFLD patients remain controversial. The participants from the Taiwan biobank cohort were included. Advanced liver fibrosis is defined as NAFLD fibrosis score (NFS) >0.675. We use carotid plaques of duplex ultrasounds to diagnose atherosclerosis. A total of 20,058 participants (age 55.67 ± 10.32; males 37.6%) were included in the final analysis. Seven thousand eight hundred and forty-three (39.1%) participants were diagnosed with MAFLD. Of them, 965 (12.3%) were lean MAFLD patients. Among lean MAFLD patients, 25.6% were comorbid with diabetes mellitus (DM). Lean MAFLD patients were older and had higher percentages of females and DM than overweight/obese MAFLD patients. After propensity score matching for age and sex, they had lower levels of NFS but a higher percentage of carotid plaques. Among four subtypes of MAFLD including “lean with DM,” “lean without DM,” “overweight/obese with DM,” and “overweight/obese without DM,” logistic regression showed that “lean with DM” subjects had the highest risk of atherosclerosis and “overweight/obese with DM” subjects had the highest risk of advanced liver fibrosis in MAFLD patients. The population-based study revealed that lean MAFLD patients make up 12.3% of all MAFLD patients, and they have a higher proportion of coexisting diabetes. Among lean MAFLD patients concurrent with diabetes, they have the highest risk of atherosclerosis and should receive special attention clinically.
{"title":"Clinical characteristics of lean metabolic-associated fatty liver disease and the impact of concurrent diabetes mellitus","authors":"Yu‐Ming Cheng, Shao-Wen Wang, Chia-Chi Wang, J. Kao","doi":"10.4103/tcmj.tcmj_253_23","DOIUrl":"https://doi.org/10.4103/tcmj.tcmj_253_23","url":null,"abstract":"ABSTRACT\u0000 \u0000 \u0000 \u0000 Metabolic-associated fatty liver disease (MAFLD) was proposed in 2020 to replace the original term nonalcoholic fatty liver disease (NAFLD) with new diagnostic criteria. The disease risks of lean and overweight/obese MAFLD patients remain controversial.\u0000 \u0000 \u0000 \u0000 The participants from the Taiwan biobank cohort were included. Advanced liver fibrosis is defined as NAFLD fibrosis score (NFS) >0.675. We use carotid plaques of duplex ultrasounds to diagnose atherosclerosis.\u0000 \u0000 \u0000 \u0000 A total of 20,058 participants (age 55.67 ± 10.32; males 37.6%) were included in the final analysis. Seven thousand eight hundred and forty-three (39.1%) participants were diagnosed with MAFLD. Of them, 965 (12.3%) were lean MAFLD patients. Among lean MAFLD patients, 25.6% were comorbid with diabetes mellitus (DM). Lean MAFLD patients were older and had higher percentages of females and DM than overweight/obese MAFLD patients. After propensity score matching for age and sex, they had lower levels of NFS but a higher percentage of carotid plaques. Among four subtypes of MAFLD including “lean with DM,” “lean without DM,” “overweight/obese with DM,” and “overweight/obese without DM,” logistic regression showed that “lean with DM” subjects had the highest risk of atherosclerosis and “overweight/obese with DM” subjects had the highest risk of advanced liver fibrosis in MAFLD patients.\u0000 \u0000 \u0000 \u0000 The population-based study revealed that lean MAFLD patients make up 12.3% of all MAFLD patients, and they have a higher proportion of coexisting diabetes. Among lean MAFLD patients concurrent with diabetes, they have the highest risk of atherosclerosis and should receive special attention clinically.\u0000","PeriodicalId":507485,"journal":{"name":"Tzu Chi Medical Journal","volume":" 47","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141680516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT The decline in bone mineral density (BMD) poses a significant concern for postmenopausal women with obesity. Research indicates that aerobic exercises show potential for enhancing bone health. However, there remains no consensus regarding their effects on BMD. This study aimed to evaluate the effect of various exercise interventions on BMD and overall health among postmenopausal women with obesity, with particular attention to caloric restriction (CR). Following Preferred Reporting Items for Systematic Reviews and Meta-Analyses 2020 guidelines, we performed a comprehensive literature search on PubMed, targeting studies published up to August 2023. Our search focused on aerobic exercise, resistance training, and combined exercise modalities, examining their impact on BMD, body composition, and physical fitness in postmenopausal women with obesity. We reviewed 11 studies, predominantly on aerobic exercise, involving women who are overweight and sedentary, nine of which were randomized trials. Our findings suggest that aerobic exercise has a mild protective effect on BMD and can significantly reduce fat mass. Notably, when combined with CR, aerobic exercise not only enhances the reduction of fat tissue mass but also potentially offers a certain level of protection for BMD. Additionally, the intervention combining aerobic exercise with resistance training emerges as a key promoter of bone health, underscoring the importance of tailored exercise programs for this population. Consequently, balanced dietary patterns (like the Mediterranean diet), combined with exercise, are recommended for optimal health outcomes. Tailored exercise programs integrating both aerobic and resistance training are crucial for sustaining overall health and bone density in this population.
{"title":"Effect of aerobic exercise on bone health in postmenopausal women with obesity: Balancing benefits with caloric restriction and resistance exercise","authors":"Ti-Yen Cheng, Wen-Tien Wu, Cheng-Huan Peng, Kuan-Lin Liu, Ting-Kuo Yao, Tzai-Chiu Yu, I. Chen, Kuang-Ting Yeh","doi":"10.4103/tcmj.tcmj_40_24","DOIUrl":"https://doi.org/10.4103/tcmj.tcmj_40_24","url":null,"abstract":"ABSTRACT\u0000 \u0000 The decline in bone mineral density (BMD) poses a significant concern for postmenopausal women with obesity. Research indicates that aerobic exercises show potential for enhancing bone health. However, there remains no consensus regarding their effects on BMD. This study aimed to evaluate the effect of various exercise interventions on BMD and overall health among postmenopausal women with obesity, with particular attention to caloric restriction (CR). Following Preferred Reporting Items for Systematic Reviews and Meta-Analyses 2020 guidelines, we performed a comprehensive literature search on PubMed, targeting studies published up to August 2023. Our search focused on aerobic exercise, resistance training, and combined exercise modalities, examining their impact on BMD, body composition, and physical fitness in postmenopausal women with obesity. We reviewed 11 studies, predominantly on aerobic exercise, involving women who are overweight and sedentary, nine of which were randomized trials. Our findings suggest that aerobic exercise has a mild protective effect on BMD and can significantly reduce fat mass. Notably, when combined with CR, aerobic exercise not only enhances the reduction of fat tissue mass but also potentially offers a certain level of protection for BMD. Additionally, the intervention combining aerobic exercise with resistance training emerges as a key promoter of bone health, underscoring the importance of tailored exercise programs for this population. Consequently, balanced dietary patterns (like the Mediterranean diet), combined with exercise, are recommended for optimal health outcomes. Tailored exercise programs integrating both aerobic and resistance training are crucial for sustaining overall health and bone density in this population.","PeriodicalId":507485,"journal":{"name":"Tzu Chi Medical Journal","volume":"227 S721","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141681388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT Diagnosing idiopathic short stature (ISS) in Traditional Chinese Medicine (TCM) remains challenging partly because the symptoms and syndrome patterns vary among ISS patients and studies. We aimed to use factor analysis of TCM symptoms to identify syndrome patterns associated with ISS in children on the basis of TCM theory. A cross-sectional study was conducted at Taipei Tzu Chi Hospital, New Taipei City, Taiwan, from October 1, 2014, to February 28, 2016. The study included 957 individuals who were newly diagnosed with ISS through simple random sampling. The outcome measures comprised 34 TCM symptoms related to children’s growth, and these symptoms were assessed using a five-level self-report questionnaire, which was completed by children and their parents. A factor analysis was conducted for the extraction of underlying factors. A total of 26 symptoms had factor loadings higher than the exact threshold value (0.4), and five factors were extracted. Factor 1, comprising seven symptoms, was interpreted as “yin deficiency and fire hyperactivity of kidney syndrome.” Factor 2, which included eight symptoms, was interpreted as “phlegm dampness stagnation of spleen syndrome.” Factor 4, which included five symptoms, was interpreted as “liver qi invading the spleen syndrome.” Factor 5, which included four symptoms, was interpreted as “spleen-stomach weakness syndrome.” Factor 3, which included four symptoms, was uninterpretable. Factors 1–5 accounted for 10%, 9%, 8%, 7%, and 6% of the total variance. Four major TCM syndrome patterns, namely, “yin deficiency and fire hyperactivity of kidney syndrome,” “phlegm dampness stagnation of spleen syndrome,” “Liver qi invading the spleen syndrome,” and “spleen–stomach weakness syndrome” were identified and accounted for 40% of the total variance of the 34 TCM symptoms surveyed in children with ISS. Our findings may facilitate the diagnosis of ISS and the optimization of treatment strategies.
{"title":"Factor analysis of traditional Chinese medicine symptoms for identification of syndrome patterns associated with idiopathic short stature in children","authors":"Tzu-Shien Liao, Li-Ping Tsai, I. Tzeng, Ya-Ting Hsu, Po-Chun Hsieh, Hsien-Chang Wu","doi":"10.4103/tcmj.tcmj_277_23","DOIUrl":"https://doi.org/10.4103/tcmj.tcmj_277_23","url":null,"abstract":"ABSTRACT\u0000 \u0000 \u0000 \u0000 Diagnosing idiopathic short stature (ISS) in Traditional Chinese Medicine (TCM) remains challenging partly because the symptoms and syndrome patterns vary among ISS patients and studies. We aimed to use factor analysis of TCM symptoms to identify syndrome patterns associated with ISS in children on the basis of TCM theory.\u0000 \u0000 \u0000 \u0000 A cross-sectional study was conducted at Taipei Tzu Chi Hospital, New Taipei City, Taiwan, from October 1, 2014, to February 28, 2016. The study included 957 individuals who were newly diagnosed with ISS through simple random sampling. The outcome measures comprised 34 TCM symptoms related to children’s growth, and these symptoms were assessed using a five-level self-report questionnaire, which was completed by children and their parents. A factor analysis was conducted for the extraction of underlying factors.\u0000 \u0000 \u0000 \u0000 A total of 26 symptoms had factor loadings higher than the exact threshold value (0.4), and five factors were extracted. Factor 1, comprising seven symptoms, was interpreted as “yin deficiency and fire hyperactivity of kidney syndrome.” Factor 2, which included eight symptoms, was interpreted as “phlegm dampness stagnation of spleen syndrome.” Factor 4, which included five symptoms, was interpreted as “liver qi invading the spleen syndrome.” Factor 5, which included four symptoms, was interpreted as “spleen-stomach weakness syndrome.” Factor 3, which included four symptoms, was uninterpretable. Factors 1–5 accounted for 10%, 9%, 8%, 7%, and 6% of the total variance.\u0000 \u0000 \u0000 \u0000 Four major TCM syndrome patterns, namely, “yin deficiency and fire hyperactivity of kidney syndrome,” “phlegm dampness stagnation of spleen syndrome,” “Liver qi invading the spleen syndrome,” and “spleen–stomach weakness syndrome” were identified and accounted for 40% of the total variance of the 34 TCM symptoms surveyed in children with ISS. Our findings may facilitate the diagnosis of ISS and the optimization of treatment strategies.\u0000","PeriodicalId":507485,"journal":{"name":"Tzu Chi Medical Journal","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141684218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT Minimally invasive surgery (MIS) has emerged as a promising alternative to conventional open techniques in the management of adolescent idiopathic scoliosis (AIS). This study presents a case series of six patients who underwent MIS for AIS at a spine center, employing a synergistic blend of intraoperative fluoroscopy, cone-beam computed tomography scans, and three-dimensional navigation technology. The surgical procedures were meticulously guided, with a focus on ensuring safety and precision in posterior pedicle fixation. Our findings demonstrate substantial correction of spinal curvature postoperatively, with a mean Cobb’s angle reduction of 51.75%. In addition, MIS facilitated a mean estimated blood loss of 241.67 ml and a mean surgical duration of 391.33 min. Comparison with conventional open techniques reveals comparable or superior outcomes in terms of correction rates, patient recovery, and opioid usage. This study underscores the potential of MIS in achieving favorable clinical outcomes while minimizing surgical impact and advancing the treatment landscape for AIS.
{"title":"Advancements in minimally invasive surgery for adolescent idiopathic scoliosis: A case series and literature review","authors":"Yi-Jen Ho, Wen-Chun Chiang, Yin-Chao Lin","doi":"10.4103/tcmj.tcmj_36_24","DOIUrl":"https://doi.org/10.4103/tcmj.tcmj_36_24","url":null,"abstract":"ABSTRACT\u0000 \u0000 \u0000 \u0000 Minimally invasive surgery (MIS) has emerged as a promising alternative to conventional open techniques in the management of adolescent idiopathic scoliosis (AIS).\u0000 \u0000 \u0000 \u0000 This study presents a case series of six patients who underwent MIS for AIS at a spine center, employing a synergistic blend of intraoperative fluoroscopy, cone-beam computed tomography scans, and three-dimensional navigation technology. The surgical procedures were meticulously guided, with a focus on ensuring safety and precision in posterior pedicle fixation.\u0000 \u0000 \u0000 \u0000 Our findings demonstrate substantial correction of spinal curvature postoperatively, with a mean Cobb’s angle reduction of 51.75%. In addition, MIS facilitated a mean estimated blood loss of 241.67 ml and a mean surgical duration of 391.33 min.\u0000 \u0000 \u0000 \u0000 Comparison with conventional open techniques reveals comparable or superior outcomes in terms of correction rates, patient recovery, and opioid usage. This study underscores the potential of MIS in achieving favorable clinical outcomes while minimizing surgical impact and advancing the treatment landscape for AIS.\u0000","PeriodicalId":507485,"journal":{"name":"Tzu Chi Medical Journal","volume":"122 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141682698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Po-Yu Huang, Bang-Gee Hsu, Chih-Hsien Wang, J. Tsai
ABSTRACT Carotid–femoral pulse wave velocity (cfPWV) is an approach primarily adopted to define arterial stiffness (AS), which is one of the major contributors to unfavorable cardiovascular outcomes. Osteopontin (OPN), in addition to regulation of bone homeostasis, is an inflammatory mediator of atherosclerosis. We performed a research which estimated the correlation between blood OPN levels and AS in participants on maintenance hemodialysis (MHD). One hundred and twenty-six patients who received long-term MHD were included in the cross-sectional study. cfPWV values were calculated based on the carotid and femoral pulsation waveforms. Patients with cfPWV >10 m/s were categorized into the AS group. We utilized a commercially available enzyme-linked immunosorbent assay to check serum concentrations of OPN. Study patients belonging to AS were found to be older, had significantly higher prevalence of underlying diabetes mellitus (DM) and hypertension, had higher systolic blood pressure, and had higher serum total calcium and OPN levels. After adjusting for these variables, multivariate logistic regression analysis disclosed that OPN levels, older age, DM, and total serum calcium levels were independently correlated with AS in patients on MHD. Multivariate analysis based on forward stepwise linear regression also showed that the logarithmically transformed OPN level was an independent correlate of cfPWV in these participants. Serum OPN concentrations had a positive correlation with cfPWV and were therefore related to AS in patients on MHD.
{"title":"Serum osteopontin level is independently associated with arterial stiffness in patients on hemodialysis","authors":"Po-Yu Huang, Bang-Gee Hsu, Chih-Hsien Wang, J. Tsai","doi":"10.4103/tcmj.tcmj_60_24","DOIUrl":"https://doi.org/10.4103/tcmj.tcmj_60_24","url":null,"abstract":"ABSTRACT\u0000 \u0000 \u0000 \u0000 Carotid–femoral pulse wave velocity (cfPWV) is an approach primarily adopted to define arterial stiffness (AS), which is one of the major contributors to unfavorable cardiovascular outcomes. Osteopontin (OPN), in addition to regulation of bone homeostasis, is an inflammatory mediator of atherosclerosis. We performed a research which estimated the correlation between blood OPN levels and AS in participants on maintenance hemodialysis (MHD).\u0000 \u0000 \u0000 \u0000 One hundred and twenty-six patients who received long-term MHD were included in the cross-sectional study. cfPWV values were calculated based on the carotid and femoral pulsation waveforms. Patients with cfPWV >10 m/s were categorized into the AS group. We utilized a commercially available enzyme-linked immunosorbent assay to check serum concentrations of OPN.\u0000 \u0000 \u0000 \u0000 Study patients belonging to AS were found to be older, had significantly higher prevalence of underlying diabetes mellitus (DM) and hypertension, had higher systolic blood pressure, and had higher serum total calcium and OPN levels. After adjusting for these variables, multivariate logistic regression analysis disclosed that OPN levels, older age, DM, and total serum calcium levels were independently correlated with AS in patients on MHD. Multivariate analysis based on forward stepwise linear regression also showed that the logarithmically transformed OPN level was an independent correlate of cfPWV in these participants.\u0000 \u0000 \u0000 \u0000 Serum OPN concentrations had a positive correlation with cfPWV and were therefore related to AS in patients on MHD.\u0000","PeriodicalId":507485,"journal":{"name":"Tzu Chi Medical Journal","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141683929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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