Pub Date : 2010-01-01DOI: 10.1201/9781420084542-c2
Jørgen M Mollerup, Thomas Budde Hansen, Søren S Frederiksen, Arne Staby
{"title":"Thermodynamic modeling of chromatographic separation.","authors":"Jørgen M Mollerup, Thomas Budde Hansen, Søren S Frederiksen, Arne Staby","doi":"10.1201/9781420084542-c2","DOIUrl":"https://doi.org/10.1201/9781420084542-c2","url":null,"abstract":"","PeriodicalId":50873,"journal":{"name":"Advances in Chromatography","volume":"48 ","pages":"57-97"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28722554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-01-01DOI: 10.1201/9781420084542-c3
Uwe D Neue, Marianna Kele, Bernard Bunner, Antonios Kromidas, Tad Dourdeville, Jeffrey R Mazzeo, Eric S Grumbach, Susan Serpa, Thomas E Wheat, Paula Hong, Martin Gilar
{"title":"Ultra-performance liquid chromatography technology and applications.","authors":"Uwe D Neue, Marianna Kele, Bernard Bunner, Antonios Kromidas, Tad Dourdeville, Jeffrey R Mazzeo, Eric S Grumbach, Susan Serpa, Thomas E Wheat, Paula Hong, Martin Gilar","doi":"10.1201/9781420084542-c3","DOIUrl":"https://doi.org/10.1201/9781420084542-c3","url":null,"abstract":"","PeriodicalId":50873,"journal":{"name":"Advances in Chromatography","volume":"48 ","pages":"99-143"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28722555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-01-01DOI: 10.1201/9781420084542-c1
Jake L. Rafferty, J. Siepmann, M. Schure
{"title":"Understanding the retention mechanism in reversed-phase liquid chromatography: insights from molecular simulation.","authors":"Jake L. Rafferty, J. Siepmann, M. Schure","doi":"10.1201/9781420084542-c1","DOIUrl":"https://doi.org/10.1201/9781420084542-c1","url":null,"abstract":"","PeriodicalId":50873,"journal":{"name":"Advances in Chromatography","volume":"48 1","pages":"1-55"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65964728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-02-23DOI: 10.1201/9781420060379.ch2
Yufeng Shen, J. Page, Richard D. Smith
The liquid chromatography (LC)-mass spectrometric (MS) analysis of peptides has become a routine method for proteomics – the study of the entire complement of proteins e.g., expressed by a cell under a specific set of conditions at a specific time. Mixtures of peptides, such as those generated from enzymatic (e.g., trypsin) digestion of globally recovered proteins (i.e. a proteome), are typically very complex and >100,000 different molecular species may be observable using MS detection [1]. LC separations implemented prior to MS for broad protein identification have three major roles: 1) to isolate individual components or reduce complexity as much as possible, 2) to increase sensitivity by concentrating the components into narrow zones prior to MS, and 3) to eliminate or displace interfering species (e.g., salts and polymers) that may be present in proteomics samples. A desired quality of LC separation can be achieved from the use of either multiple steps of moderate quality separations, or fewer steps of high power separations. The former approach is generally more easily accessible for very high quality separations due to the variety of commercialized LC platforms available, while the latter still often requires considerable developmental efforts (for both columns and instrumentation). In addition tomore » proteomics data quality, other differences between these two approaches include proteomics analysis time and sample consumption (and subsequent analysis costs), as well as direct impact on potential proteomics applications that have special requirements in terms of analysis coverage, sample size, dynamic range, sensitivity, and throughput.« less
{"title":"Advanced capillary liquid chromatography-mass spectrometry for proteomics","authors":"Yufeng Shen, J. Page, Richard D. Smith","doi":"10.1201/9781420060379.ch2","DOIUrl":"https://doi.org/10.1201/9781420060379.ch2","url":null,"abstract":"The liquid chromatography (LC)-mass spectrometric (MS) analysis of peptides has become a routine method for proteomics – the study of the entire complement of proteins e.g., expressed by a cell under a specific set of conditions at a specific time. Mixtures of peptides, such as those generated from enzymatic (e.g., trypsin) digestion of globally recovered proteins (i.e. a proteome), are typically very complex and >100,000 different molecular species may be observable using MS detection [1]. LC separations implemented prior to MS for broad protein identification have three major roles: 1) to isolate individual components or reduce complexity as much as possible, 2) to increase sensitivity by concentrating the components into narrow zones prior to MS, and 3) to eliminate or displace interfering species (e.g., salts and polymers) that may be present in proteomics samples. A desired quality of LC separation can be achieved from the use of either multiple steps of moderate quality separations, or fewer steps of high power separations. The former approach is generally more easily accessible for very high quality separations due to the variety of commercialized LC platforms available, while the latter still often requires considerable developmental efforts (for both columns and instrumentation). In addition tomore » proteomics data quality, other differences between these two approaches include proteomics analysis time and sample consumption (and subsequent analysis costs), as well as direct impact on potential proteomics applications that have special requirements in terms of analysis coverage, sample size, dynamic range, sensitivity, and throughput.« less","PeriodicalId":50873,"journal":{"name":"Advances in Chromatography","volume":"47 1","pages":"31-58"},"PeriodicalIF":0.0,"publicationDate":"2009-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65964298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-01-01DOI: 10.1201/9781420060379.ch6
A. Staby, Jacob Nielsen, J. Krarup, Matthias Wiendahl, T. Hansen, Steffen Kidal, J. Hubbuch, J. Mollerup
{"title":"Advances in resins for ion-exchange chromatography","authors":"A. Staby, Jacob Nielsen, J. Krarup, Matthias Wiendahl, T. Hansen, Steffen Kidal, J. Hubbuch, J. Mollerup","doi":"10.1201/9781420060379.ch6","DOIUrl":"https://doi.org/10.1201/9781420060379.ch6","url":null,"abstract":"","PeriodicalId":50873,"journal":{"name":"Advances in Chromatography","volume":"47 1","pages":"193-245"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65964312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-01-01DOI: 10.1201/9781420060263.ch6
David V McCalley
{"title":"Liquid chromatographic separations of basic compounds.","authors":"David V McCalley","doi":"10.1201/9781420060263.ch6","DOIUrl":"https://doi.org/10.1201/9781420060263.ch6","url":null,"abstract":"","PeriodicalId":50873,"journal":{"name":"Advances in Chromatography","volume":"46 ","pages":"305-50"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27842953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Enhanced-fluidity liquid mixtures: fundamental properties and chromatography.","authors":"Susan V Olesik","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":50873,"journal":{"name":"Advances in Chromatography","volume":"46 ","pages":"423-49"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27842956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-01-01DOI: 10.1201/9781420060263.ch4
Naijun Wu
Enantioselective separation by supercritical fluid chromatography (SFC) has been a field of great progress since the first demonstration of a chiral separation by SFC in the 1980s. The unique properties of supercritical fluids make packed column SFC the most favorable choice for fast enantiomeric separation among all of the separation techniques. In this chapter, the effect of chiral stationary phases, modifiers, and additives on enantioseparation are discussed in terms of speed and resolution in SFC. Fundamental considerations and thermodynamic aspects are also presented.
{"title":"Increasing speed of enantiomeric separations using supercritical fluid chromatography.","authors":"Naijun Wu","doi":"10.1201/9781420060263.ch4","DOIUrl":"https://doi.org/10.1201/9781420060263.ch4","url":null,"abstract":"<p><p>Enantioselective separation by supercritical fluid chromatography (SFC) has been a field of great progress since the first demonstration of a chiral separation by SFC in the 1980s. The unique properties of supercritical fluids make packed column SFC the most favorable choice for fast enantiomeric separation among all of the separation techniques. In this chapter, the effect of chiral stationary phases, modifiers, and additives on enantioseparation are discussed in terms of speed and resolution in SFC. Fundamental considerations and thermodynamic aspects are also presented.</p>","PeriodicalId":50873,"journal":{"name":"Advances in Chromatography","volume":"46 ","pages":"213-34"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27842951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-01-01DOI: 10.1201/9781420060263.ch10
Danielle Ryan, Philip Marriott
{"title":"Comprehensive two-dimensional gas chromatography.","authors":"Danielle Ryan, Philip Marriott","doi":"10.1201/9781420060263.ch10","DOIUrl":"https://doi.org/10.1201/9781420060263.ch10","url":null,"abstract":"","PeriodicalId":50873,"journal":{"name":"Advances in Chromatography","volume":"46 ","pages":"451-67"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27842957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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