Arachidonic acid (ARA) is an essential fatty acid with numerous biological activities that benefits human health. However, ARA-rich phosphatidylcholine (PtdCho), which has a higher bioavailability than ARA-rich triacylglycerols, is scarce in the natural source. In this study, we developed an enzymatic modification approach for the synthesis of ARA-rich PtdCho from ARA-rich ethyl esters (EE). The maximum incorporation of ARA into PtdCho (24.02%) was achieved from the optimized conditions, including ARA-rich EE/PtdCho mass ratio of 2:1, hexane, lipase Novozym 435 as a biocatalyst (15% of enzyme load) and reaction time of 24 h. The 31P nuclear magnetic resonance (NMR) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) results revealed that the PtdCho content decreased to 17.53% and the ARA-containing PtdCho species was primarily identified as PtdCho (18:2/20:4). Taken together, this investigation offers a new reference for the efficient production of ARA-rich PtdCho via enzymatic modification and paves a theoretical groundwork of industrial production practice.
花生四烯酸（ARA）是一种必需脂肪酸，具有多种生物活性，有益于人类健康。然而，与富含 ARA 的三酰甘油相比，富含 ARA 的磷脂酰胆碱（PtdCho）具有更高的生物利用率，但在天然来源中却很少见。在这项研究中，我们开发了一种从富含 ARA 的乙酯（EE）合成富含 ARA 的 PtdCho 的酶修饰方法。优化条件包括富含 ARA 的 EE/PtdCho 质量比为 2:1、正己烷、生物催化剂脂肪酶 Novozym 435（酶载量的 15%）和 24 小时的反应时间。31P 核磁共振（NMR）和液相色谱-串联质谱（LC-MS/MS）分析结果显示，PtdCho 含量下降到 17.53%，含 ARA 的 PtdCho 物种主要被鉴定为 PtdCho（18:2/20:4）。综上所述，这项研究为通过酶法改性高效生产富含 ARA 的 PtdCho 提供了新的参考，并为工业化生产实践奠定了理论基础。
{"title":"Enzymatic Synthesis of Arachidonic Acid-Rich Phosphatidylcholine from Arachidonic Acid Ethyl Esters","authors":"Jingnan Lei, Jiajia Gong, Yuan He, Shuang Zhu, Chin-Ping Tan, Y. Liu, Yong‐jiang Xu","doi":"10.37256/fse.5120243637","DOIUrl":"https://doi.org/10.37256/fse.5120243637","url":null,"abstract":"Arachidonic acid (ARA) is an essential fatty acid with numerous biological activities that benefits human health. However, ARA-rich phosphatidylcholine (PtdCho), which has a higher bioavailability than ARA-rich triacylglycerols, is scarce in the natural source. In this study, we developed an enzymatic modification approach for the synthesis of ARA-rich PtdCho from ARA-rich ethyl esters (EE). The maximum incorporation of ARA into PtdCho (24.02%) was achieved from the optimized conditions, including ARA-rich EE/PtdCho mass ratio of 2:1, hexane, lipase Novozym 435 as a biocatalyst (15% of enzyme load) and reaction time of 24 h. The 31P nuclear magnetic resonance (NMR) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) results revealed that the PtdCho content decreased to 17.53% and the ARA-containing PtdCho species was primarily identified as PtdCho (18:2/20:4). Taken together, this investigation offers a new reference for the efficient production of ARA-rich PtdCho via enzymatic modification and paves a theoretical groundwork of industrial production practice.","PeriodicalId":513460,"journal":{"name":"Food Science and Engineering","volume":"42 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139531923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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