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Physicochemical properties, total phenolic content, and antioxidant activity of the honey samples from the Herzegovina region 黑塞哥维那地区蜂蜜样品的理化特性、总酚含量和抗氧化活性
Pub Date : 2024-05-05 DOI: 10.7251/agren2401033k
Maja Kazazic, E. Mehić
Honey is a natural product that is an excellent source of energy containing mainly carbohydrates and water, as well as small amounts of organic acids, vitamins, minerals, flavonoids, and enzymes. Due to the presence of bioactive compounds, it has been shown that honey is beneficial for many diseases, e.g. gastrointestinal diseases, skin diseases, cancer, heart diseases, and neurological degeneration. The study of the physical and chemical properties of honey and the content of bioactive compounds has been increasingly applied in order to determine the quality of honey samples. The aim of this study is to investigate physicochemical properties as well as the total phenol content and antioxidant activity of seven multifloral honey samples from the Herzegovina region. Physicochemical parameters determined in the honey samples (moisture, acidity, electrical conductivity, reducing sugars, sucrose, and insoluble matter) were within the quality standard limits of the Regulation on methods for control of honey and other bee products. Total phenolic content was determined using the Folin-Ciocalteu method and it ranged from 46.98 ± 6.36 to 152.94 ± 4.95 mg GAE/100 g of honey. To determine the antioxidant activity of the honey samples, two methods, FRAP and ABTS, were used. The total phenolic content of honey correlated positively with its antioxidant activity.
蜂蜜是一种天然产品,是极好的能量来源,主要含有碳水化合物和水分,以及少量有机酸、维生素、矿物质、类黄酮和酶。由于含有生物活性化合物,蜂蜜对许多疾病都有益处,如胃肠道疾病、皮肤病、癌症、心脏病和神经退化。为了确定蜂蜜样品的质量,对蜂蜜的物理和化学性质以及生物活性化合物含量进行研究的应用越来越广泛。本研究的目的是调查来自黑塞哥维那地区的七种多花蜂蜜样品的理化性质、总酚含量和抗氧化活性。蜂蜜样品中测定的理化参数(水分、酸度、电导率、还原糖、蔗糖和不溶物)均符合《蜂蜜和其他蜂产品控制方法条例》规定的质量标准限值。总酚含量采用 Folin-Ciocalteu 法测定,其范围为 46.98 ± 6.36 至 152.94 ± 4.95 毫克 GAE/100 克蜂蜜。为了测定蜂蜜样品的抗氧化活性,采用了 FRAP 和 ABTS 两种方法。蜂蜜的总酚含量与其抗氧化活性呈正相关。
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引用次数: 0
Significance of the apiary location for the content of copper and zinc in the bee pollen 养蜂场所在地对蜂花粉中铜和锌含量的影响
Pub Date : 2024-05-05 DOI: 10.7251/agren2401001m
Goran Mirjanić, Dijana Mihajlović, B. Bosancic
Bee pollen is one of the most valuable direct products of honey bees. Bees as well as humans use pollen in their nutrition as a high-quality supplement. Therefore, information about the mineral content of pollen is valuable. The aim of this study was to determine the amounts of copper (Cu) and zinc (Zn) in the bee pollen collected from the three locations (Kozarska Dubica, Petrovo, and Srbac). Quantification of copper and zinc was done by atomic absorption spectrophotometry after acid digestion (HNO3+HClO4) of pollen. The highest contents of both elements were found in the bee pollen from Kozarska Dubica (8.41 mg Cu/kg, 29.52 mg Zn/kg). The lowest Cu content was found in the samples from Petrovo (6.19 mg/kg), while the lowest Zn content was determined in the pollen from Srbac (22.06 mg/kg). Additionally, there were statistically significant differences in the content of both elements between pollen originating from different locations. As the location of the apiary was mainly determined by its proximity to potential sources of metal contamination, botanical origin of pollen and technology of pollen collection, it is important to investigate their impact in more detail on the elemental content of pollen in the future.
蜂花粉是蜜蜂最宝贵的直接产品之一。蜜蜂和人类都将花粉作为高质量的营养补充剂。因此,有关花粉矿物质含量的信息非常宝贵。本研究旨在确定从三个地点(Kozarska Dubica、Petrovo 和 Srbac)采集的蜂花粉中铜(Cu)和锌(Zn)的含量。花粉经酸性消化(HNO3+HClO4)后,采用原子吸收分光光度法对铜和锌进行定量。在 Kozarska Dubica 的蜂花粉中,这两种元素的含量最高(8.41 毫克铜/千克,29.52 毫克锌/千克)。来自 Petrovo 的样本中铜含量最低(6.19 毫克/千克),而来自 Srbac 的花粉中锌含量最低(22.06 毫克/千克)。此外,来自不同地点的花粉在这两种元素的含量上存在显著的统计学差异。由于养蜂场的位置主要取决于其是否靠近潜在的金属污染源、花粉的植物来源和花粉采集技术,因此今后有必要更详细地研究它们对花粉元素含量的影响。
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引用次数: 0
The influence of humus extract on the quality of raspberry (Rubus idaeus L.) 腐殖土提取物对覆盆子(Rubus idaeus L.)品质的影响
Pub Date : 2024-05-05 DOI: 10.7251/agren2401011i
M. Ičanović, Sabira Handanović, Mihajlo Marković
This paper analyzes the influence of foliar fertilizer based on humus extract on some of the elements central to the quality of the Polka raspberry variety in the area of the city of Bihać. The research was conducted in 2015 according to the control and treatment system. A foliar fertilizer based on humus extract was used for the treatment. A total of 12 quantitative and qualitative properties were analyzed: content of total sugars, reducing sugars, invert sugars, sucrose, water content, dry matter, total acidity, vitamin C, total phenols, total flavonoids, antioxidant capacity, and fruit mass. After the analyses were completed, it can be concluded that fruits of raspberry plants treated with humus extract have an almost identical value of total acidity (1.65%) as found in control. However, treatment with a humus extract slightly lowered the content of total phenols and vitamin C. Based on the results obtained, it was shown that the foliar fertilizer based on the humus extract did not have a positive effect on certain chemical and antioxidant properties of the raspberry fruit.
本文分析了基于腐殖质提取物的叶面肥对比哈奇市地区波尔卡树莓品种品质的一些核心要素的影响。研究于2015年按照对照和处理系统进行。对照组采用基于腐殖质提取物的叶面肥。共分析了 12 项定量和定性特性:总糖、还原糖、转化糖、蔗糖、含水量、干物质、总酸度、维生素 C、总酚、总黄酮、抗氧化能力和果实质量。分析结果表明,用腐殖质提取物处理过的树莓果实的总酸度值(1.65%)几乎与对照组相同。根据获得的结果表明,基于腐殖土提取物的叶面肥对树莓果实的某些化学和抗氧化特性没有积极影响。
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引用次数: 0
The relationship between gluten proteins and loaf volume 面筋蛋白与面包体积的关系
Pub Date : 2024-05-05 DOI: 10.7251/agren2401045k
D. Knežević, D. Kondić, Aleksandra. Yu. Dragovich, Alexander M. Kudryavtsev, Mirela Matković Stojšin, V. Zečević, A. Radosavac, A. Paunović
The storage proteins influence technological quality value of grain wheat, dough quality traits, and loaf quality. The aim of this study is to estimate variability in the dry gluten content, loaf volume, and their relationships with encoding alleles of gliadin and glutenins. Wheat genotypes grown in two vegetation seasons (2015/16 and 2016/17) were studied. The technological quality traits analyzed in this study varied in wheat genotypes within and between vegetation seasons. In both vegetation seasons the highest dry gluten content was established in G-3621-1 (30.23% and 31.15%) and the highest value of loaf volume in G-3621-1 (530 ml and 540 ml). In both vegetation seasons the least dry gluten content was found in G-3606-6 (25.42% and 25.98%) and the least loaf volume in G-3606-6 (380 ml and 390 ml). The composition of gliadin and glutenin alleles in the wheat genotypes analyzed was different. The genotypes carrying Gli-B1b, Gli-D1b, Gli-D2b, and Glu-A1b, Glu-B1c, Glu-D1d had the highest gluten content, while the genotype that carried Gli-B1l and Glu-A1b, Glu-B1c, Glu-D1d had high bread volume. The results have shown relationships between gliadin and glutenin alleles and quality traits of grain, flour, and bread.
贮藏蛋白会影响谷物小麦的技术质量价值、面团质量性状和面包质量。本研究旨在估计干面筋含量、面包体积的变异性及其与麦胶蛋白和谷蛋白编码等位基因的关系。研究了在两个植被季节(2015/16和2016/17)种植的小麦基因型。本研究分析的技术质量性状在植被季节内和植被季节间的小麦基因型中各不相同。在两个植被季节中,G-3621-1 的干面筋含量最高(30.23% 和 31.15%),G-3621-1 的面包体积值最高(530 毫升和 540 毫升)。在两个植被季节中,G-3606-6 的干面筋含量最低(25.42% 和 25.98%),面包体积最小(380 毫升和 390 毫升)。所分析的小麦基因型中的麦胶蛋白和谷蛋白等位基因的组成各不相同。携带 Gli-B1b、Gli-D1b、Gli-D2b 和 Glu-A1b、Glu-B1c、Glu-D1d 的基因型面筋含量最高,而携带 Gli-B1l 和 Glu-A1b、Glu-B1c、Glu-D1d 的基因型面包体积大。研究结果表明,谷胶蛋白和谷朊蛋白等位基因与谷物、面粉和面包的品质性状之间存在关系。
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引用次数: 0
The influence of geographical area on morphometric parameters of the honey bee in Serbia 地理区域对塞尔维亚蜜蜂形态参数的影响
Pub Date : 2024-05-05 DOI: 10.7251/agren2401021i
Aleksandar Ignjatović, Kazimir Matović, Goran Jevtić, N. Nedić
In this study, morphological characteristics were measured on worker honey bee samples collected from seven different locations covering the territory of the Republic of Serbia. Three samples were taken from the territory of AP Vojvodina, namely from the Southern Banat District (Vršac; I), the South Bačka District (Bač; II), and the West Bačka District (Crvenka; III). The worker bees from the central Serbia originated from different sites namely, the Rasina District (Aleksandrovac; IV), the Zlatibor District (Lučani; V), Pešter (Sjenica; VI), and the Bor District (Negotin; VII). From each sample, 15 bees were randomly selected, and their front right wings were dissected and mounted on slides in two rows. After wing preparation, they were scanned using an Epson Perfection V600 Photo scanner with a resolution of 4800 dpi. The measurement included a total of 14 parameters, 11 of which were the angles on the forewing, while the other parameters analyzed were the forewing length and width and the cubital index. The results have shown that there is a high statistically significant difference between the localities in the characteristics examined in this study. From a total of 11 angles analyzed, statistical significance (p0.01) of average values between the localities was determined for the following angles: A4, J16, K19, L13, N23, G18, and O26. The analysis of the results of the front wings length and the cubital index has shown a significant difference between the groups of honey bees. The average forewing length across all locations in the study was 9.17mm, while the average forewing width was 3.43. The difference in terms of the average forewing width was not statistically significant between the locations. The values of the cubital index ranged from 2.28 to 2.79, with an average of 2.49 across all locations.
本研究测量了从塞尔维亚共和国境内七个不同地点采集的工蜂样本的形态特征。三个样本来自伏伊伏丁那自治省,即南巴纳特区(弗尔沙茨;I)、南巴卡区(巴奇;II)和西巴卡区(克尔文卡;III)。塞尔维亚中部的工蜂来自不同地点,即拉西纳区(Aleksandrovac;IV)、兹拉蒂博尔区(Lučani;V)、佩什特尔(Sjenica;VI)和博尔区(Negotin;VII)。从每个样本中随机抽取 15 只蜜蜂,将其右前翅解剖并安装在载玻片上,分成两排。翅膀准备完毕后,使用分辨率为 4800 dpi 的 Epson Perfection V600 照片扫描仪对其进行扫描。测量共包括 14 个参数,其中 11 个为前翅角度,其他分析参数为前翅长度和宽度以及立方指数。结果表明,本研究考察的特征在各地之间存在很大的统计学差异。在总共分析的 11 个角度中,以下角度的平均值在各地之间具有统计学意义(P0.01):A4、J16、K19、L13、N23、G18 和 O26。对蜜蜂前翅长度和立方体指数的分析结果表明,各组蜜蜂之间存在显著差异。研究中所有地点的蜜蜂前翅平均长度为 9.17 毫米,前翅平均宽度为 3.43 毫米。不同地点之间平均前翅宽度的差异在统计学上并不显著。立方指数的范围在 2.28 至 2.79 之间,所有地点的平均值为 2.49。
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