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Spore Diversity of Arbuscular Mycorrhizal Fungi in Upo Wetland 蒲坡湿地丛枝菌根真菌孢子多样性研究
Q4 Environmental Science Pub Date : 2019-01-01 DOI: 10.4489/KJM.20190003
K. Ko, Hyeok Park, K. Ka, Eom Ahnheum
We extracted arbuscular mycorrhizal fungal (AMF) spores from rhizospheres of three plants from Upo Wetland, Korea. We identified the isolated AMF spores based on morphological characteristics and phylogenetic analysis of partial 18S rDNA nucleotide sequences. The species diversity of AMF spores was calculated among the study sites and host plants. Consequently, nine species from six genera of AMF spores were identified. We confirmed the species diversity of the AMF spores in rhizospheres affected by host plants in the wetland. In the course of this study, we confirmed a previously unreported AMF species in Korea: Diversispora epigaea. We described the morphological features and molecular characteristics of this previously unreported AMF species.
从韩国乌浦湿地3株植物的根际提取了丛枝菌根真菌(AMF)孢子。我们根据形态特征和部分18S rDNA核苷酸序列的系统发育分析鉴定了分离的AMF孢子。计算了研究地点和寄主植物间AMF孢子的物种多样性。结果鉴定出AMF孢子6属9种。我们证实了湿地根际真菌孢子受寄主植物影响的物种多样性。在本研究过程中,我们确认了一种以前未报道的韩国AMF物种:diverspora epigaea。我们描述了这种以前未报道的AMF物种的形态特征和分子特征。
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引用次数: 0
Confirmation of Coleosporium zanthoxyli on Zanthoxylum ailanthoides in Korea 韩国花椒属花椒孢子的确证
Q4 Environmental Science Pub Date : 2019-01-01 DOI: 10.4489/KJM.20190010
H. Shin, Young‐Joon Choi, C. K. Lee, Ho Sang Lee, W. Choi
A previous record of Coleosporium phellodendri on Zanthoxylum ailanthoides in Korea by Korean researchers was regarded as C. zanthoxyli by foreign scientists without further studies. To clarify the identity of a Coleosporium species occurring on Z. ailanthoides in Korea, we examined the morphological characteristics of eight materials and analyzed the internal transcribed spacer (ITS) and 28S large subunit (LSU) rDNA regions of three representative specimens which were collected in Korea and deposited in the Mycological Herbarium at Korea University (Seoul, Korea). All specimens collected in Korea were morphologically and molecularly determined to be C. zanthoxyli. Therefore, this is the first confirmed report on the presence of C. zanthoxyli on Z. ailanthoides in Korea.
此前,国内研究人员在花椒上发现了黄斑病孢(Coleosporium phellodendri),但外国科学家认为这是花椒孢(C. zanthoxyli),没有进行进一步的研究。为了明确一种发生在韩国的高丽大学真菌学植物标本室的绿芽孢属植物的身份,研究了8种材料的形态特征,并分析了在韩国采集的3个代表性标本的内部转录间隔区(ITS)和28S大亚基(LSU) rDNA区域。所有在韩国采集的标本经形态学和分子鉴定均为花椒。因此,这是国内首次确认臭椿属植物中含有花椒属植物。
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引用次数: 1
First Report of Leptosphaerulina saccharicola Isolated from Persimmon (Diospyros kaki ) Tree Bark in Korea 韩国柿子(Diospyros kaki)树皮中分离的糖化细粒绦虫首次报道
Q4 Environmental Science Pub Date : 2019-01-01 DOI: 10.4489/KJM.20190002
Okouma Nguia Fulbert, Benjamin Yaw Ayim, Kallol Das, Y. Lim, Seung-Yeol Lee, Jung, Hee Young
A fungal strain, designated PTT-2, was isolated from the bark of the trunk of a persimmon (Diospyros kaki) tree in Cheongdo, Korea. The isolate showed morphological similarities with Leptosphaerulina saccharicola. Strain PTT-2 had more rapid growth on potato dextrose agar medium than on oatmeal agar, malt extract agar, and synthetic nutrient poor agar media, with colony sizes of 53.8 mm, 49.8 mm, 48.4 mm, and 28.1 mm after 7 days at 25℃ temperature, respectively. Strain PTT-2 produced ascospores, which had irregular wavy edges, oblong to ellipsoidal shape, hyaline appearance and 23.6 × 10 μm size. The black ascomata were developed on PDA medium, and asci were recorded. A BLAST search of the internal transcribed spacer (ITS) region, TEF1-α and RPB2 gene sequences revealed that strain PTT-2 showed more than 99% nucleotide similarity with a strain of Leptosphaerulina saccharicola previously reported from Thailand. A neighbor-joining phylogenetic tree was constructed by concatenating the above-mentioned sequences, and showed that strain PTT-2 clustered in the same clade with L. saccharicola. Based on these findings, this is the first record of Leptosphaerulina saccharicola occurring in Korea.
从韩国清道一棵柿树(Diospyros kaki)树干的树皮中分离出一种真菌菌株,命名为PTT-2。该分离物与糖化细螺旋体形态相似。菌株PTT-2在马铃薯葡萄糖琼脂培养基上的生长速度快于在燕麦琼脂、麦芽提取物琼脂和合成营养不良琼脂培养基上的生长速度,在25℃温度下培养7 d,菌落大小分别为53.8 mm、49.8 mm、48.4 mm和28.1 mm。菌株PTT-2产生的子囊孢子边缘不规则,呈波浪状,长圆形至椭球状,外观透明,大小为23.6 × 10 μm。在PDA培养基上培养黑色腹膜,并记录腹膜。内部转录间隔区(ITS)、TEF1-α和RPB2基因序列的BLAST搜索结果显示,菌株PTT-2与先前报道的泰国细螺旋藻(leptosphaulina saccharicola)菌株核苷酸相似性超过99%。将上述序列串联起来,构建了一个相邻连接的系统发育树,结果表明菌株PTT-2与L. saccharicola聚在同一支系。根据这些发现,这是韩国首次记录到糖化细螺旋体。
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引用次数: 0
Basil Tree, a New Host of Downy Mildew Pathogen Peronospora belbahrii 霜霉病病菌Peronospora belbahrii的新宿主罗勒树
Q4 Environmental Science Pub Date : 2018-09-01 DOI: 10.4489/KJM.20180031
Hyun Ju Lee, Jae Sung Lee, H. Shin, Young‐Joon Choi
Basil (Ocimum spp.) is a popular herb grown worldwide. During the past fifteen years, a downy mildew pathogen has caused considerable damage to basil cultivations. In August 2017, downy mildew disease symptoms were found on Basil Tree (or long foot Basil Tree), which was developed by the grafting of two basil varieties and is a continuous harvest plant with a woody trunk. The present study reports the occurrence of downy mildew disease in basil Tree and identifies the causal pathogen, as Peronospora belbahrii.
罗勒是一种在世界各地种植的受欢迎的草本植物。在过去的十五年里,一种霜霉菌病原体对罗勒的种植造成了相当大的破坏。2017年8月,在罗勒树(或长脚罗勒树)上发现霜霉病症状,罗勒树是由两个罗勒品种嫁接而成,是一种连续收获的木质树干植物。本研究报道了罗勒树霜霉菌病的发生,并确定了致病菌为belbahrii Peronospora。
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引用次数: 0
Induction of dectin-1 expression and intracellular signal transduction by β-Glucan of ganoderma lucidum β-葡聚糖诱导灵芝dectin-1表达及细胞内信号转导
Q4 Environmental Science Pub Date : 2018-06-01 DOI: 10.4489/KJM.20180019
H. Ryu, Ha Won Kim
Fungal β-glucan, known to have immunostimulatory and antitumor activities, can be recognized by host immune cells as one of the pathogen-associated molecular patterns (PAMPs). Although there are several reports on the diverse immunostimulatory activities of β-glucan, little is known about the intracellular signal transduction of β-glucan. Stimulation of RAW264.7 macrophage cells with β-glucan from Ganoderma lucidum induced the expressions of dectin-1, toll-like receptor 2 (TLR2), TLR4, and TLR6 at the transcription stage. Treatment with β-glucan also induced inflammatory mediators such as macrophage inflammatory proteins (MIP)-1α, MIP-1β, MIP-1γ, interleukin (IL)-1β, and tumor necrosis factor (TNF)-α. Treatment of the cells with polymyxin B, an inhibitor of lipopolysaccharides (LPS), blocked the induction of inflammatory mediators in LPSor β-glucan-stimulated systems. Pretreatment of the cells in our cell culture system with LY294002, a phosphoinositide 3-kinase (PI3K) inhibitor, or U0126, a mitogen-activated protein kinase/extracellular-signal-regulated kinase (MAPK/ERK) kinase (MEK)1/MEK2 inhibitor, led to a reduction in the induction of inflammatory mediators in a concentration-dependent manner. These results show that stimulation of the macrophage cells by β-glucan induced the expressions of both dectin-1 and TLRs. We also found that the PI3K/Akt and MEK pathways were involved in the induction of inflammatory mediators in macrophage cells during intracellular signal transduction of β-glucan.
真菌β-葡聚糖具有免疫刺激和抗肿瘤活性,可被宿主免疫细胞识别为病原体相关分子模式(PAMP)之一。尽管有几篇关于β-葡聚糖不同免疫刺激活性的报道,但对β-葡聚糖的细胞内信号转导知之甚少。灵芝β-葡聚糖刺激RAW264.7巨噬细胞,在转录阶段诱导dectin-1、toll样受体2(TLR2)、TLR4和TLR6的表达。β-葡聚糖治疗还诱导炎症介质,如巨噬细胞炎症蛋白(MIP)-1α、MIP-1β、MIP-1γ、白细胞介素(IL)-1β和肿瘤坏死因子(TNF)-α。用脂多糖(LPS)抑制剂多粘菌素B处理细胞,阻断了LPSorβ-葡聚糖刺激系统中炎症介质的诱导。在我们的细胞培养系统中,用磷酸肌醇3-激酶(PI3K)抑制剂LY294002或有丝分裂原活化蛋白激酶/细胞外信号调节激酶(MAPK/ERK)激酶(MEK)1/MEK2抑制剂U0126预处理细胞,导致炎症介质的诱导以浓度依赖的方式减少。这些结果表明,β-葡聚糖对巨噬细胞的刺激诱导了dectin-1和TLRs的表达。我们还发现,在β-葡聚糖的细胞内信号转导过程中,PI3K/Akt和MEK途径参与了巨噬细胞炎症介质的诱导。
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引用次数: 1
Podosphaera pannosa causes powdery mildew and rusty spot on peach fruits from Korea 韩国的桃果会发生白粉病和锈斑
Q4 Environmental Science Pub Date : 2018-06-01 DOI: 10.4489/KJM.20180022
H. Shin, Sung-Eun Cho, I. Choi, Kyoung-Won Seo
The fungus, Podosphaera pannosa, was identified in 1991 as the cause of powdery mildew symptoms on peach (Prunus persica var. persica) fruit from Korea based on the morphological characteristics of the conidial state. Recently, however, in Serbia and France, the cause of ‘rusty spot’ found on peach fruit was identified as P. leucotricha, and the cause of ‘powdery mildew’ on nectarine (Prunus persica var. nucipersica) fruit was identified as P. pannosa. To confirm the identity of the Korean pathogen, we collected four samples of powdery mildew from Korean peach fruit: three with the ‘powdery mildew’ symptom and one with the ‘rusty spot’ symptom. Morphological examination of the four samples confirmed P. pannosa as the pathogen. Internal transcribed spacer sequences of rDNA were analyzed for molecular characterization. A phylogenetic tree showed that the Korean isolates were clustered into a clade containing P. pannosa from Rosa species, with high sequence similarities of more than 99%. Thus, we showed that the powdery mildew and rusty spot symptoms on peach fruits from Korea are associated with P. pannosa.
这种真菌Podosphaera pannosa于1991年根据分生孢子状态的形态特征被确定为韩国桃子(Prunus persica var. persica)果实白粉病症状的原因。然而,最近在塞尔维亚和法国,在桃子果实上发现的“锈斑”被确定为P. leucotricha,而油桃果实上的“白粉病”被确定为P. pannosa。为了确认韩国病原菌的身份,我们从韩国桃果中采集了4份白粉病样品,其中3份具有“白粉病”症状,1份具有“锈斑”症状。形态学检查证实这4份标本为病原菌。分析rDNA的内部转录间隔序列进行分子表征。系统发育树分析结果表明,韩国分离株与蔷薇属的pannosa属同源分支,序列相似性超过99%。因此,我们证明了韩国桃子果实上的白粉病和锈斑症状与pannosa有关。
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引用次数: 0
Identification of erysiphe izuensis on rhododendron yedoense f. poukhanense in Korea based on morphological and molecular characteristics 基于形态和分子特征的韩国普氏杜鹃上的伊苏丹毒鉴定
Q4 Environmental Science Pub Date : 2018-03-01 DOI: 10.4489/KJM.20180009
Sung-Eun Cho, Sang-Hyun Lee, Sun Keun Lee, S. Seo, H. Shin
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引用次数: 1
Diversity of Foliar Endophytic Fungi Inhabiting Coniferous Trees in Korea 韩国针叶树叶面内生真菌的多样性
Q4 Environmental Science Pub Date : 2018-01-01 DOI: 10.4489/KJM.20180036
J. Eo, Ahn-Heum Eom
Fungal endophytes are defined as fungi inhabiting plant tissues, causing no apparent disease. As the agricultural and ecological importance of endophytes has increased, many studies have been performed on various aspects, ranging from basic identification, taxonomy, and evolution to secondary metabolites with potential for human use. In the Korean Peninsula, conifers are the most commonly found evergreen trees, with approximately 30 to 60 reported taxa. Studies on the effect of conifer-associated endophytes on the host plants are required for the preservation and conservation of coniferous forests which decline by climate change and deforestation. This review summarizes the diversity of endophytic fungi in coniferous trees of Korea and their relationship with host plants.
真菌内生菌是指寄生在植物组织中,不引起明显疾病的真菌。随着内生菌在农业和生态方面的重要性日益增加,从基本鉴定、分类和进化到具有潜在人类利用潜力的次生代谢物等各个方面都进行了许多研究。在朝鲜半岛,针叶树是最常见的常绿乔木,大约有30至60个分类群。研究针叶林相关内生菌对寄主植物的影响是保护和保护因气候变化和森林砍伐而减少的针叶林的必要条件。本文综述了韩国针叶树内生真菌的多样性及其与寄主植物的关系。
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引用次数: 2
Mycelial Culture and Fruiting Analysis of Panellus edulis Strains Collected in Korea 韩国产毛茛菌丝培养及结果分析
Q4 Environmental Science Pub Date : 2018-01-01 DOI: 10.4489/KJM.20180039
Sung-I Woo, Rhim Ryoo, Y. Jang, Youngae Park, Yeun Sug Jeong, K. Ka
Molecular analysis using the internal transcribed spacer region sequences revealed that the strains used in this study, which were formerly identified as Panellus serotinus, are Panellus edullis. After Universal Fungal PCR Fingerprinting (UFPF) analysis, eight strains of P. edulis were divided into two groups. We conducted fundamental research on mycelial growth and sawdust cultivation to understand the cultural characteristics of eight wild P. edulis strains collected from Korean forests. All strains showed faster and denser mycelial growth on potato dextrose agar (PDA) than on other media (malt extract agar, Sabouraud dextrose agar). Optimal conditions for mycelial growth were: 20°C on PDA, 25°C on potato dextrose broth (PDB), and pH 5~8 on PDB at 25°C. Two strains (NIFoS 2407, 3993) were selected as excellent strains based on mycelial growth and density on PDA. NIFoS 2792 showed high cellulase activities on carboxymethyl cellulose (CMC) agar, and NIFoS 2387 and 2804 exhibited high laccase activities on ABTS-containing agar media. The mycelial growth of P. edulis was the fastest on Quercus acutissima and Q. mongolica sawdust media, and mycelial density was the highest on Quercus spp. sawdust-containing media. Sawdust cultivation of P. edulis was successful. The conditions were 80~85 days of cultivation period after spawn inoculation, 10~11 days for primordial formation at 17~18°C, and 15~20 days for fruiting growth. NIFoS 2804 and 3993 were selected as good strains in terms of cultivation period and mushroom production. These results could be useful for the artificial cultivation of P. edulis.
利用内部转录间隔区序列的分子分析表明,本研究中使用的菌株,以前鉴定为Panellus sertinus,是Panellus edullis。经通用真菌PCR指纹图谱(UFPF)分析,将8株毛利假单胞菌分为两组。为了了解从韩国森林中采集的8株野生毛竹菌株菌丝生长和锯末培养的基本特性,我们进行了菌丝生长和锯末培养的基础研究。所有菌株在马铃薯葡萄糖琼脂(PDA)上的菌丝生长速度和密度均高于其他培养基(麦芽膏琼脂、沙乌德葡萄糖琼脂)。菌丝生长的最佳条件为:PDA 20°C,马铃薯葡萄糖肉汤(PDB) 25°C, PDB在25°C下pH 5~8。根据菌丝生长情况和PDA上菌丝密度选择2株菌株(NIFoS 2407、3993)为优良菌株。NIFoS 2792在羧甲基纤维素(CMC)琼脂上表现出较高的纤维素酶活性,NIFoS 2387和2804在含abts的琼脂培养基上表现出较高的漆酶活性。毛竹在麻栎和蒙古栎木屑培养基上菌丝生长最快,菌丝密度在栎木屑培养基上最高。木屑栽培毛竹是成功的。接种菌种后培养80~85 d,在17~18℃条件下初始形成10~11 d,果实生长15~20 d。从栽培期和蘑菇产量两方面选择NIFoS 2804和3993为较好的菌种。这些结果对毛竹的人工栽培具有一定的指导意义。
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引用次数: 0
Microbiological Characteristics of Alcoholfermenting Wild Yeast, Aureobasidium pullulans P-1 and Its Makgeolli Fermentation Characteristics 醇发酵野生酵母普鲁兰毛霉P-1微生物学特性及其米酒发酵特性
Q4 Environmental Science Pub Date : 2018-01-01 DOI: 10.4489/KJM.20180026
Yong-Cheol Hong, Sangmin Han, Jongsoo Lee
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引用次数: 0
期刊
Korean Journal of Mycology
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