Pub Date : 2021-01-01DOI: 10.2208/JOURNALOFJSCE.9.1_86
Kanji Nakamura, M. Fukuda
Two types of transformants with di ff erent drug resistance were developed from the phenol-oxidizing bacterium, Cupriavidus sp. KN1. The first transformant is Cupriavidus sp. KN1-TGF that has green fluorescence and tetracycline resistance. The second transformant is Cupriavidus sp. KN1-KRF that has red fluorescence and kanamycin resistance. These two strains can be selectively colonized with antibiotic-containing media, and they can be also distinguished by fluorescent color. First, Cupriavidus sp. KN1-TGF cells remaining after predation with the flagellate, Spumella sp. TGKK2, were mixed with Cupriavidus sp. KN1-KRF that did not contact with the flagellate TGKK2 to investigate the change in characteristics for the protistan predation. As a result, Cupriavidus sp. KN1-KRF was preferentially predated. On the other hand, Cupriavidus sp. KN1-TGF in the same tube was relatively resistant to predation. Similar phenomena were observed when the conditions of these two strains were reversed. Next, two complete mixing reactors connected in series were operated. Bacteria were cultured in the first reactor, and the protistan predation was made in the second reactor. Two runs were operated, one with Cupriavidus sp. KN1-TGF and the other with Cupriavidus sp. KN1-KRF. Then the first reactor e ffl uent that contains Cupriavidus sp. KN1-KRF was mixed with the second reactor e ffl uent that contains residual Cupriavidus sp. KN1-TGF and the flagellate TGKK2 in a tube. The cells of Cupriavidus sp. KN1-KRF not in contact with the protist were preferentially predated. This phenomenon was similarly confirmed by combinatorial inversion that Cupriavidus sp. KN1-TGF from the first reactor was mixed with Cupriavidus sp. KN1-KRF from the second reactor.
{"title":"BACTERIAL GRAZING-RESISTANCE DEVELOPED DURING CO-EXISTENCE WITH A BACTERIVOROUS PROTIST","authors":"Kanji Nakamura, M. Fukuda","doi":"10.2208/JOURNALOFJSCE.9.1_86","DOIUrl":"https://doi.org/10.2208/JOURNALOFJSCE.9.1_86","url":null,"abstract":"Two types of transformants with di ff erent drug resistance were developed from the phenol-oxidizing bacterium, Cupriavidus sp. KN1. The first transformant is Cupriavidus sp. KN1-TGF that has green fluorescence and tetracycline resistance. The second transformant is Cupriavidus sp. KN1-KRF that has red fluorescence and kanamycin resistance. These two strains can be selectively colonized with antibiotic-containing media, and they can be also distinguished by fluorescent color. First, Cupriavidus sp. KN1-TGF cells remaining after predation with the flagellate, Spumella sp. TGKK2, were mixed with Cupriavidus sp. KN1-KRF that did not contact with the flagellate TGKK2 to investigate the change in characteristics for the protistan predation. As a result, Cupriavidus sp. KN1-KRF was preferentially predated. On the other hand, Cupriavidus sp. KN1-TGF in the same tube was relatively resistant to predation. Similar phenomena were observed when the conditions of these two strains were reversed. Next, two complete mixing reactors connected in series were operated. Bacteria were cultured in the first reactor, and the protistan predation was made in the second reactor. Two runs were operated, one with Cupriavidus sp. KN1-TGF and the other with Cupriavidus sp. KN1-KRF. Then the first reactor e ffl uent that contains Cupriavidus sp. KN1-KRF was mixed with the second reactor e ffl uent that contains residual Cupriavidus sp. KN1-TGF and the flagellate TGKK2 in a tube. The cells of Cupriavidus sp. KN1-KRF not in contact with the protist were preferentially predated. This phenomenon was similarly confirmed by combinatorial inversion that Cupriavidus sp. KN1-TGF from the first reactor was mixed with Cupriavidus sp. KN1-KRF from the second reactor.","PeriodicalId":52233,"journal":{"name":"Journal of Japan Society of Civil Engineers","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68181990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.2208/JSCEJIPM.76.5_I_909
Sho Takakusagi, Nobuaki Ohmori, Teppei Osada
{"title":"ACCESSIBILITY OF CHILD-REARING HOUSEHOLDS: COMBINATION OF ESCORTING CHILDREN TO AFTER-SCHOOL ACTIVITIES AND GROCERY SHOPPING","authors":"Sho Takakusagi, Nobuaki Ohmori, Teppei Osada","doi":"10.2208/JSCEJIPM.76.5_I_909","DOIUrl":"https://doi.org/10.2208/JSCEJIPM.76.5_I_909","url":null,"abstract":"","PeriodicalId":52233,"journal":{"name":"Journal of Japan Society of Civil Engineers","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73956506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.2208/JSCEJIPM.77.2_97
Anna Nagasako, Hajime Watanabe, Yoshihiro Sato, T. Maruyama
{"title":"IMPACTS OF POST-DISASTER RESIDENTIAL LOCATIONS ON THE DISASTER PUBLIC HOUSING LOCATION PREFERENCES OF VICTIMS IN MASHIKI FOLLOWING THE 2016 KUMAMOTO EARTHQUAKE","authors":"Anna Nagasako, Hajime Watanabe, Yoshihiro Sato, T. Maruyama","doi":"10.2208/JSCEJIPM.77.2_97","DOIUrl":"https://doi.org/10.2208/JSCEJIPM.77.2_97","url":null,"abstract":"","PeriodicalId":52233,"journal":{"name":"Journal of Japan Society of Civil Engineers","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85010489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.2208/JSCEJIPM.76.5_I_739
Masahiro Tada, Mizuki Koide, M. Nishida, K. Iida, H. Sawada
{"title":"AN ANALYSIS OF DRIVING CHARACTERISTICS WHILE USING SEMI-AUTONOMOUS DRIVING SYSTEM BASED ON PHYSIOLOGICAL INDEX AND GAZE BAHAVIOR","authors":"Masahiro Tada, Mizuki Koide, M. Nishida, K. Iida, H. Sawada","doi":"10.2208/JSCEJIPM.76.5_I_739","DOIUrl":"https://doi.org/10.2208/JSCEJIPM.76.5_I_739","url":null,"abstract":"","PeriodicalId":52233,"journal":{"name":"Journal of Japan Society of Civil Engineers","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85387639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.2208/JSCEJIPM.76.5_I_531
Ibuki Higuchi, Tomohide Okada, Yousuke Tajima, Hiroyuki Samizo, N. Yokouchi
{"title":"A STUDY ON FEASIBILITY EVALUATION OF WATERFRONT DEVELOPMENT -FOCUSING ON THE BUSINESS PLANS AND REALIZATION STATUS OF “PORT RENAISSANCE 21” AND “MARINE TOWN PROJECT”-","authors":"Ibuki Higuchi, Tomohide Okada, Yousuke Tajima, Hiroyuki Samizo, N. Yokouchi","doi":"10.2208/JSCEJIPM.76.5_I_531","DOIUrl":"https://doi.org/10.2208/JSCEJIPM.76.5_I_531","url":null,"abstract":"","PeriodicalId":52233,"journal":{"name":"Journal of Japan Society of Civil Engineers","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81786191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.2208/JSCEJIPM.76.5_I_757
Shohei Yasuda, K. Ikeda, T. Iryo, Masaaki Ishihara
{"title":"DEMAND CURVE ESTIMATION METHOD FOR DESCRIBING DEMAND FLUCTUATION ON URBAN EXPRESSWAY","authors":"Shohei Yasuda, K. Ikeda, T. Iryo, Masaaki Ishihara","doi":"10.2208/JSCEJIPM.76.5_I_757","DOIUrl":"https://doi.org/10.2208/JSCEJIPM.76.5_I_757","url":null,"abstract":"","PeriodicalId":52233,"journal":{"name":"Journal of Japan Society of Civil Engineers","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82216864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.2208/JSCEJIPM.77.2_62
H. Baba, Y. Akiyama, Osamu Yachida
{"title":"DEVELOPING PREDICTIVE MODEL FOR VACANT HOUSING DISTRIBUTION USING MUNICIPALITY-OWNED DATA —CASE STUDY IN MAEBASHI CITY—","authors":"H. Baba, Y. Akiyama, Osamu Yachida","doi":"10.2208/JSCEJIPM.77.2_62","DOIUrl":"https://doi.org/10.2208/JSCEJIPM.77.2_62","url":null,"abstract":"","PeriodicalId":52233,"journal":{"name":"Journal of Japan Society of Civil Engineers","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81049704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.2208/JSCEJIPM.76.5_I_339
Yoshiho Tanaka, Tetsuji Sato
{"title":"PRODUCTIVITY EXPANSION EFFECT OF INFRASTRUCTURES AFTER THE HIGH ECONOMIC GROWTH PERIOD IN JAPAN","authors":"Yoshiho Tanaka, Tetsuji Sato","doi":"10.2208/JSCEJIPM.76.5_I_339","DOIUrl":"https://doi.org/10.2208/JSCEJIPM.76.5_I_339","url":null,"abstract":"","PeriodicalId":52233,"journal":{"name":"Journal of Japan Society of Civil Engineers","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90100692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.2208/JSCEJIPM.76.5_I_977
Tomoki Yoshida, M. Okumura
{"title":"OPTIMAL NETWORK STRUCTURE OF INTERCITY PASSENGER TRANSPORTATION CONSIDERING COST SHARING SCHEMES","authors":"Tomoki Yoshida, M. Okumura","doi":"10.2208/JSCEJIPM.76.5_I_977","DOIUrl":"https://doi.org/10.2208/JSCEJIPM.76.5_I_977","url":null,"abstract":"","PeriodicalId":52233,"journal":{"name":"Journal of Japan Society of Civil Engineers","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89715765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.2208/JSCEJIPM.76.5_I_657
Akira Mitarai, Ryo Komatsuzaki, M. Taniguchi
{"title":"PROVIDING NEW URBAN FUNCTIONS TO ACCOMMODATE SPREADING MOBILITY INNOVATION-FOCUS ON ADVUS (AUTOMATED DRIVING VEHICLE WITH UTILITY SERVICE)-","authors":"Akira Mitarai, Ryo Komatsuzaki, M. Taniguchi","doi":"10.2208/JSCEJIPM.76.5_I_657","DOIUrl":"https://doi.org/10.2208/JSCEJIPM.76.5_I_657","url":null,"abstract":"","PeriodicalId":52233,"journal":{"name":"Journal of Japan Society of Civil Engineers","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89546485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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