N. Şener, Z. Demirel, E. Imamoglu, Meltem Conk Dalay
Commercial carotenoids of green microalgae have become significant especially for their applications in the cosmetic, pharmaceutical, food and feed industries. Effects of physical and chemical parameters on carotenoid contents in isolated microalgal species have been investigated. The variables of shaking rate, nitrogen concentration and light intensity affect biomass production and the synthesis of carotenoids in the green microalgae were investigated using the statistical design by Box-Behnken (BBD) employing Response Surface Methodology (RSM). Furthermore, the optimized cultivation conditions using BBD for Chlorella vulgaris, Ankistrodesmus convolutus, Dunaliel-la salina, Tetraselmis striata were determined using the spectrophotometric method to enhance carotenoid concentration. A. convolutus within the green algae was detected with the highest carotenoid concentration. The optimum conditions results indicated that the growth of A. convolutus (0.55 mg/L) and production of total carotenoids (25.1138 mg/g biomass) were found at the stirrer rate of 100 rpm under the light intensity of 100 μE/m 2 s, and in the nutrient component of 8.82 mM NaNO 3 . These conditions were validated experimentally for total carotenoid yield (24.13 mg/g biomass). After that the production was performed in a flat-plate photobioreactor with a volume of 6L based on the optimized conditions and the carotenoid profile was defined by HPLC-DAD using standards such as violaxanthin, astaxanthin and β -carotene. This study proposes that the RSM approach can be used to define optimal conditions for large-scale production of carotenoids by A. convolutus .
商业化的绿色微藻类胡萝卜素已经变得非常重要，尤其是在化妆品、制药、食品和饲料工业中的应用。研究了理化参数对分离微藻类胡萝卜素含量的影响。采用响应面法（RSM），采用Box-Behnken（BBD）的统计设计，研究了振荡速率、氮浓度和光照强度等变量对绿色微藻生物量产生和类胡萝卜素合成的影响。此外，采用分光光度法测定了小球藻（Chlorella vulgaris）、盘藻（Ankistrodesmus convolutus）、盐藻（Dunaliel la salina）、条纹藻（Tetraselmis striata）的最佳BBD培养条件，以提高类胡萝卜素的浓度。在绿藻中检测到具有最高类胡萝卜素浓度的旋毛虫。结果表明，在光照强度为100μE/m2s、搅拌速度为100rpm、营养成分为8.82mM NaNO3的条件下，旋花A.convolultus的生长量为0.55mg/L，总类胡萝卜素的产生量为25.1138mg/g。通过实验验证了这些条件的类胡萝卜素总产量（24.13mg/g生物量）。然后，基于优化的条件，在体积为6L的平板光生物反应器中进行生产，并通过HPLC-DAD使用紫罗兰红素、虾青素和β-胡萝卜素等标准品定义类胡萝卜素图谱。本研究提出，RSM方法可用于确定A.convolultus大规模生产类胡萝卜素的最佳条件。
{"title":"Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus","authors":"N. Şener, Z. Demirel, E. Imamoglu, Meltem Conk Dalay","doi":"10.26650/ase2020785091","DOIUrl":"https://doi.org/10.26650/ase2020785091","url":null,"abstract":"Commercial carotenoids of green microalgae have become significant especially for their applications in the cosmetic, pharmaceutical, food and feed industries. Effects of physical and chemical parameters on carotenoid contents in isolated microalgal species have been investigated. The variables of shaking rate, nitrogen concentration and light intensity affect biomass production and the synthesis of carotenoids in the green microalgae were investigated using the statistical design by Box-Behnken (BBD) employing Response Surface Methodology (RSM). Furthermore, the optimized cultivation conditions using BBD for Chlorella vulgaris, Ankistrodesmus convolutus, Dunaliel-la salina, Tetraselmis striata were determined using the spectrophotometric method to enhance carotenoid concentration. A. convolutus within the green algae was detected with the highest carotenoid concentration. The optimum conditions results indicated that the growth of A. convolutus (0.55 mg/L) and production of total carotenoids (25.1138 mg/g biomass) were found at the stirrer rate of 100 rpm under the light intensity of 100 μE/m 2 s, and in the nutrient component of 8.82 mM NaNO 3 . These conditions were validated experimentally for total carotenoid yield (24.13 mg/g biomass). After that the production was performed in a flat-plate photobioreactor with a volume of 6L based on the optimized conditions and the carotenoid profile was defined by HPLC-DAD using standards such as violaxanthin, astaxanthin and β -carotene. This study proposes that the RSM approach can be used to define optimal conditions for large-scale production of carotenoids by A. convolutus .","PeriodicalId":52866,"journal":{"name":"Aquatic Sciences and Engineering","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49347182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seher Dirican, Aydin Kaleli, E. Yilmaz, A. Özer, H. Dayıoğlu
{"title":"New Records of Diatoms (Bacillariales, Rhopalodiales & Surirellales) with ultrastructure details from the Black Sea Coast of Turkey","authors":"Seher Dirican, Aydin Kaleli, E. Yilmaz, A. Özer, H. Dayıoğlu","doi":"10.26650/ase2021983449","DOIUrl":"https://doi.org/10.26650/ase2021983449","url":null,"abstract":"","PeriodicalId":52866,"journal":{"name":"Aquatic Sciences and Engineering","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47916034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Mazumder, M. Salam, A. Khan, S. Khalil, Tawheed Hasan
{"title":"Biological and Reproductive Indices of Mystus bleekeri (Day, 1877) in Open Water Body (Dekhar Haor) of Bangladesh","authors":"S. Mazumder, M. Salam, A. Khan, S. Khalil, Tawheed Hasan","doi":"10.26650/ase2021979813","DOIUrl":"https://doi.org/10.26650/ase2021979813","url":null,"abstract":"","PeriodicalId":52866,"journal":{"name":"Aquatic Sciences and Engineering","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45579844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
By catch is a serious conservation challenge for the fisheries whose viability is increasingly under threat. To approach maximum by catch reduction with minimum loss of targeted catch, fisheries need to have information on the environmental and anthropogenic factors in multi species seas like the Mediterranean. In this study, we used generalized additive models (GAM) to by the catch ratio of purse seine fishery to determine the effects of environmental variables. The data were collected during each fishing trip in the 2018-2019 fishing season that covers the time period between September 1st and April 15th. There were 26 species (66.216 mt in total) recorded as by catch and the rates of by catch species in the total by catch amount varied between 0.001% and 23.1%. In terms of habitat of by catch species, the total ratios of benthopelagic, demersal and pelagic species were 52% , 28% and 20%, respectively. Significant interactions observed indicate that the fluctuations in by catch ratios differed by depth and sea surface temperature, whereas the quarters of year and the moon phases were not found to affect by catch ratios significantly.
{"title":"Relationship Between by Catch Ratio of Sardine-Anchovy Targeted Purse Seine and Some Environmental Factors Based on a General Addictive Model in the Aegean Sea","authors":"Z. Tosunoğlu","doi":"10.26650/ase2021963166","DOIUrl":"https://doi.org/10.26650/ase2021963166","url":null,"abstract":"By catch is a serious conservation challenge for the fisheries whose viability is increasingly under threat. To approach maximum by catch reduction with minimum loss of targeted catch, fisheries need to have information on the environmental and anthropogenic factors in multi species seas like the Mediterranean. In this study, we used generalized additive models (GAM) to by the catch ratio of purse seine fishery to determine the effects of environmental variables. The data were collected during each fishing trip in the 2018-2019 fishing season that covers the time period between September 1st and April 15th. There were 26 species (66.216 mt in total) recorded as by catch and the rates of by catch species in the total by catch amount varied between 0.001% and 23.1%. In terms of habitat of by catch species, the total ratios of benthopelagic, demersal and pelagic species were 52% , 28% and 20%, respectively. Significant interactions observed indicate that the fluctuations in by catch ratios differed by depth and sea surface temperature, whereas the quarters of year and the moon phases were not found to affect by catch ratios significantly.","PeriodicalId":52866,"journal":{"name":"Aquatic Sciences and Engineering","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44230185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Monitoring of Biochemical Effects of Phenol in the Carp (Cyprinus carpio) Fry","authors":"Mikail Özcan, Ü. İspir","doi":"10.26650/ase2021864312","DOIUrl":"https://doi.org/10.26650/ase2021864312","url":null,"abstract":"","PeriodicalId":52866,"journal":{"name":"Aquatic Sciences and Engineering","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41361333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Melek Işinibilir Okyar, Esin Yüksel Durmaz, S. O. Güreşen
The present study represents the first record of Hydrozoan medusae Geryonia proboscidalis off of Gökçeada island in June 2021. Although this species is found in the Mediterranean, it is the first record specifically in the coastal areas of Gökçeada island. Holidaymakers are extremely irritated by the sting of Geryonia proboscidalis , especially with its sudden accumulation in the coastal areas of Gökçeada island
{"title":"The First Record of Geryonia proboscidalis (Forskål, 1775), (Cnidaria: Hydrozoa) on the Coasts of Gökçeada, the Aegean Sea, Turkey","authors":"Melek Işinibilir Okyar, Esin Yüksel Durmaz, S. O. Güreşen","doi":"10.26650/ase2021962514","DOIUrl":"https://doi.org/10.26650/ase2021962514","url":null,"abstract":"The present study represents the first record of Hydrozoan medusae Geryonia proboscidalis off of Gökçeada island in June 2021. Although this species is found in the Mediterranean, it is the first record specifically in the coastal areas of Gökçeada island. Holidaymakers are extremely irritated by the sting of Geryonia proboscidalis , especially with its sudden accumulation in the coastal areas of Gökçeada island","PeriodicalId":52866,"journal":{"name":"Aquatic Sciences and Engineering","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42559042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Faculty of Aquatic Sciences, Department of Fisheries and Seafood Processing Technology","authors":"Didem Üçok Alakavuk, Suhendan Mol","doi":"10.26650/ase2021928254","DOIUrl":"https://doi.org/10.26650/ase2021928254","url":null,"abstract":"","PeriodicalId":52866,"journal":{"name":"Aquatic Sciences and Engineering","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48919660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Environmental concerns have been raised because of a garbage disposal facility (GDF) constructed near Çavuşlu Stream in Giresun, Turkey. This article proposes a fully validated ICP-MS technique to investigate the impacts of the GDF on the water quality of the stream and provide a human health risk estimation. Water sampling was carried out at four different stations and in tap water since the stream is the primary source of drinking water for the inhabitants of the town. Quantification of selected toxic metals (As, Pb, Cd, Hg, Sb, Al, and Ni) in conjunction with the essential and other elements (Se, Cu, Fe, Mg, Mn, Zn, and Co) was performed by the use of the previously validated ICP-MS method. Once water quality index (WQI), heavy metal pollution index (HPI), and heavy metal evaluation index (HEI) were computed, the health risk assessment was studied according to the US EPA’s method. Although the stations (2, 3, 4, and tap water) showed excellent water quality, station-1, which is the closest one to the facility, was classified as poor water quality. Lifetime cancer risk (LCR) was only significant both for adults and children in station-1. In addition, low risk regarding non-carcinogenic health hazards was found for children. The results indicate that the facility decreases the water quality of this stream and is possibly responsible for LCR. In conclusion, the ecological environment and human health should be protected by further monitoring the effect of the GDF on the ecological system.
{"title":"Impacts of a Garbage Disposal Facility on the Water Quality of Çavuşlu Stream in Giresun, Turkey: A Health Risk Assessment Study by a Validated ICP-MS Assay","authors":"B. Yüksel, F. Ustaoğlu, E. Arıca","doi":"10.26650/ase2020845246","DOIUrl":"https://doi.org/10.26650/ase2020845246","url":null,"abstract":"Environmental concerns have been raised because of a garbage disposal facility (GDF) constructed near Çavuşlu Stream in Giresun, Turkey. This article proposes a fully validated ICP-MS technique to investigate the impacts of the GDF on the water quality of the stream and provide a human health risk estimation. Water sampling was carried out at four different stations and in tap water since the stream is the primary source of drinking water for the inhabitants of the town. Quantification of selected toxic metals (As, Pb, Cd, Hg, Sb, Al, and Ni) in conjunction with the essential and other elements (Se, Cu, Fe, Mg, Mn, Zn, and Co) was performed by the use of the previously validated ICP-MS method. Once water quality index (WQI), heavy metal pollution index (HPI), and heavy metal evaluation index (HEI) were computed, the health risk assessment was studied according to the US EPA’s method. Although the stations (2, 3, 4, and tap water) showed excellent water quality, station-1, which is the closest one to the facility, was classified as poor water quality. Lifetime cancer risk (LCR) was only significant both for adults and children in station-1. In addition, low risk regarding non-carcinogenic health hazards was found for children. The results indicate that the facility decreases the water quality of this stream and is possibly responsible for LCR. In conclusion, the ecological environment and human health should be protected by further monitoring the effect of the GDF on the ecological system.","PeriodicalId":52866,"journal":{"name":"Aquatic Sciences and Engineering","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46858198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Is Gambusia holbrooki Known as an Invasive Fish Species in Küçük Menderes River Basin Lakes (Turkey)?","authors":"I. Kurtul, H. Sarı","doi":"10.26650/ase2020755178","DOIUrl":"https://doi.org/10.26650/ase2020755178","url":null,"abstract":"","PeriodicalId":52866,"journal":{"name":"Aquatic Sciences and Engineering","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41718861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Different Shaking Systems on the Growth of Marine Diatom Phaeodactylum Tricornutum","authors":"Sevil Deniz Yakan Dündar, Oktay Türeyen","doi":"10.26650/ase2021905122","DOIUrl":"https://doi.org/10.26650/ase2021905122","url":null,"abstract":"","PeriodicalId":52866,"journal":{"name":"Aquatic Sciences and Engineering","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44153950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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