Abdul Gani Soulissa, B. Lombardo, Armelia Sari Widyarman
Background: Periodontal disease is one of the most prevalent oral health problems in Indonesia that affects supporting tissues of the teeth. Porphyromonas gingivalis plays an important role in the pathogenesis of periodontal disease. Alternative therapy with natural plant extracts, including pineapple (Ananas comosus) hump extract may inhibit the growth of bacteria that cause periodontal disease. Objective: To determine the effect of pineapple hump extract on bacterial growth and adhesion of Porphyromonas gingivalis biofilms. Method: The bacterial inhibition test was performed by the agar well diffusion method, and biofilm density measurements were made using the biofilm assay method. Results: Pineapple hump extract can inhibit bacterial growth optimally at a concentration of 100%, with an average zone of inhibition of 7.3 mm. The extract at a 50% concentration can eradicate the biofilms in a 6 h incubation time with an average OD of 0.124. The Shapiro-Wilk method confirmed a normal distribution of the data. Both one-way ANOVA and post hoc test showed a significant difference between the inhibitory ability of pineapple hump extract and between concentrations to restrict formation of Porphyromonas gingivalis biofilms. Conclusion: Pineapple hump extract was able to inhibit bacterial growth and eradicate the adhesion of Porphyromonas gingivalis biofilms in vitro.
{"title":"Antibacterial and Antibiofilm Efficacy of Pineapple Hump (Ananas comosus) on Porphyromonas gingivalis in vitro","authors":"Abdul Gani Soulissa, B. Lombardo, Armelia Sari Widyarman","doi":"10.14693/jdi.v28i3.1267","DOIUrl":"https://doi.org/10.14693/jdi.v28i3.1267","url":null,"abstract":"Background: Periodontal disease is one of the most prevalent oral health problems in Indonesia that affects supporting tissues of the teeth. Porphyromonas gingivalis plays an important role in the pathogenesis of periodontal disease. Alternative therapy with natural plant extracts, including pineapple (Ananas comosus) hump extract may inhibit the growth of bacteria that cause periodontal disease. Objective: To determine the effect of pineapple hump extract on bacterial growth and adhesion of Porphyromonas gingivalis biofilms. Method: The bacterial inhibition test was performed by the agar well diffusion method, and biofilm density measurements were made using the biofilm assay method. Results: Pineapple hump extract can inhibit bacterial growth optimally at a concentration of 100%, with an average zone of inhibition of 7.3 mm. The extract at a 50% concentration can eradicate the biofilms in a 6 h incubation time with an average OD of 0.124. The Shapiro-Wilk method confirmed a normal distribution of the data. Both one-way ANOVA and post hoc test showed a significant difference between the inhibitory ability of pineapple hump extract and between concentrations to restrict formation of Porphyromonas gingivalis biofilms. Conclusion: Pineapple hump extract was able to inhibit bacterial growth and eradicate the adhesion of Porphyromonas gingivalis biofilms in vitro.","PeriodicalId":53873,"journal":{"name":"Journal of Dentistry Indonesia","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43921623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Deciding on what treatment (repair or replacement) to provide for a failed indirect restoration can be challenging. Notably, the strength of the residual tooth structure could be improved after replacing the failed indirect restoration using a minimally invasive strategy. Objective: To describe the use of a minimally invasive strategy for the successful clinical replacement of two failed indirect metal restorations with direct composite restorations and the attainment of bonded restoration. Case Report: Case 1. A 52-year-old male patient came to the dental hospital complaining of discomfort when drinking cold beverages and food impaction on the proximal area of the upper left first molar. Case 2. A 45-year-old female patient complained about her debonded metal onlay and secondary caries in the upper right second molar. Both of these indirect restoration failures were treated with direct resin composite restoration. Both patients were highly satisfied with the results. Conclusion: This report presents the benefits of adopting a minimally invasive strategy approach for replacing failed indirect restorations (inlays and onlays) with direct-bonded composite restorations. Minimally invasive direct restorations are designed to maximize the preservation of tooth structure, thereby ensuring a long-term bonded restoration and leaving future options open when a failure occurs.
{"title":"Replacement of Two Failed Indirect Restorations with Direct Bonded Restorations Using Minimally Invasive Strategy","authors":"Citra Kusumasari, Takashi Hatayama, Y. Shimada","doi":"10.14693/jdi.v28i3.1304","DOIUrl":"https://doi.org/10.14693/jdi.v28i3.1304","url":null,"abstract":"Deciding on what treatment (repair or replacement) to provide for a failed indirect restoration can be challenging. Notably, the strength of the residual tooth structure could be improved after replacing the failed indirect restoration using a minimally invasive strategy. Objective: To describe the use of a minimally invasive strategy for the successful clinical replacement of two failed indirect metal restorations with direct composite restorations and the attainment of bonded restoration. Case Report: Case 1. A 52-year-old male patient came to the dental hospital complaining of discomfort when drinking cold beverages and food impaction on the proximal area of the upper left first molar. Case 2. A 45-year-old female patient complained about her debonded metal onlay and secondary caries in the upper right second molar. Both of these indirect restoration failures were treated with direct resin composite restoration. Both patients were highly satisfied with the results. Conclusion: This report presents the benefits of adopting a minimally invasive strategy approach for replacing failed indirect restorations (inlays and onlays) with direct-bonded composite restorations. Minimally invasive direct restorations are designed to maximize the preservation of tooth structure, thereby ensuring a long-term bonded restoration and leaving future options open when a failure occurs.","PeriodicalId":53873,"journal":{"name":"Journal of Dentistry Indonesia","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43289422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Candida albicans has a number of properties, including resistance to various antimicrobial agents, which allow it to survive in the root canals. Lactobacillus reuteri plays a role in maintaining oral health through interactions with the oral microbiome. L. reuteri has potential as a preventive and therapeutic agent against inflammatory diseases. Objective: The aim of this study was to investigate the effect of irrigation with a reuterin-containing solution on BCR1, ACE2, EFG1, and TEC1 gene expression in C. albicans root canal biofilms. Methods: L. reuteri was cultured in MRS broth and incubated anaerobically for 24 hours at 37°C. C. albicans was cultured in Sabouraud dextrose broth at 37°C for 48 hours. A total of 24 single-rooted premolar teeth were standardized and inoculated with C. albicans before irrigation with 50 μg/mL reuterin as a single, independent variable (Indonesian strain), 50 μg/mL reuterin Prodentis (a strain combination of L. reuteri DSM 17938 and L. reuteri ATCC PTA 5289), 2.5% sodium hypochlorite as positive control, and saline as negative control. A real-time quantitative polymerase chain reaction (RT-qPCR) assay was used to detect the expression of BCR1, ACE2, EFG1, and TEC1 in C. albicans root canal biofilms. Results: Reuterin significantly reduced the expression of BCR1 and ACE2 genes, which play a role in C. albicans biofilm formation, at the biofilm maturation stage (P < 0.05). Reuterin also affected the expression of the EFG1 and TEC1 genes, although the effect was not significant. Conclusion: A reuterin isolate of L. reuteri exhibits antibiofilm activity against the expression of C. albicans genes involved in biofilm formation. Reuterin has potential as an irrigation agent in the treatment of root canals. Further research is needed to shed light on the effectiveness of reuterin against the expression of genes that play important roles in the formation of C. albicans biofilms.
{"title":"The Effect of Parabiotic Reuterin on the Expression of Genes Involved in Candida albicans Biofilm Formation: An Ex vivo Study","authors":"Anastasya Muna Riad, Armelia Sari Widyarman","doi":"10.14693/jdi.v28i3.1276","DOIUrl":"https://doi.org/10.14693/jdi.v28i3.1276","url":null,"abstract":"Candida albicans has a number of properties, including resistance to various antimicrobial agents, which allow it to survive in the root canals. Lactobacillus reuteri plays a role in maintaining oral health through interactions with the oral microbiome. L. reuteri has potential as a preventive and therapeutic agent against inflammatory diseases. Objective: The aim of this study was to investigate the effect of irrigation with a reuterin-containing solution on BCR1, ACE2, EFG1, and TEC1 gene expression in C. albicans root canal biofilms. Methods: L. reuteri was cultured in MRS broth and incubated anaerobically for 24 hours at 37°C. C. albicans was cultured in Sabouraud dextrose broth at 37°C for 48 hours. A total of 24 single-rooted premolar teeth were standardized and inoculated with C. albicans before irrigation with 50 μg/mL reuterin as a single, independent variable (Indonesian strain), 50 μg/mL reuterin Prodentis (a strain combination of L. reuteri DSM 17938 and L. reuteri ATCC PTA 5289), 2.5% sodium hypochlorite as positive control, and saline as negative control. A real-time quantitative polymerase chain reaction (RT-qPCR) assay was used to detect the expression of BCR1, ACE2, EFG1, and TEC1 in C. albicans root canal biofilms. Results: Reuterin significantly reduced the expression of BCR1 and ACE2 genes, which play a role in C. albicans biofilm formation, at the biofilm maturation stage (P < 0.05). Reuterin also affected the expression of the EFG1 and TEC1 genes, although the effect was not significant. Conclusion: A reuterin isolate of L. reuteri exhibits antibiofilm activity against the expression of C. albicans genes involved in biofilm formation. Reuterin has potential as an irrigation agent in the treatment of root canals. Further research is needed to shed light on the effectiveness of reuterin against the expression of genes that play important roles in the formation of C. albicans biofilms.","PeriodicalId":53873,"journal":{"name":"Journal of Dentistry Indonesia","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48299944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Numerous studies have been conducted on the effect of various beverages on the mechanical properties of toothcolored materials, however, little is known about the effects of these materials on composites, particularly newer types such as nanocomposites. Objectives: This study aimed to evaluate the surface microhardness of two types of composites, micro-hybrid (point 4) and nanohybrid (premise), after exposure to natural and industrial juices. Methods: In this experimental study, 90 disc-shaped specimens with a thickness of 2 mm and a diameter of 10 mm were taken from two composites, micro-hybrid Point4 (Kerr) and nanohybrid premise (kerr) (two groups of 45). Then, the specimens of each group were divided into 5 subgroups of 9 and were immersed for 7 days for 6 hours in 5 solutions of industrial orange juice, natural orange juice, industrial pomegranate juice, natural pomegranate juice, and distilled water (control group). Surface microhardness of specimens was measured by Vickers device at baseline, one day and one week after immersing. Data was measured by ANOVA, repeated measure test, and independent t-test. A significant level of α was 0.05. Results: The surface microhardness of two types of composites exposed to beverages was reduced significantly. However, no significant difference was found between natural and industrial juices in none of the composites. Conclusions: Natural and industrial juices can affect the surface microhardness of composites, which varies depending on the type of composite and the type of juice and immersion time.
{"title":"Effect of Natural and Industrial Juices on Surface Microhardness of Microhybrid and Nanohybrid Composites","authors":"R. Meshki, Hanieh Hoseini","doi":"10.14693/jdi.v28i2.1173","DOIUrl":"https://doi.org/10.14693/jdi.v28i2.1173","url":null,"abstract":"Numerous studies have been conducted on the effect of various beverages on the mechanical properties of toothcolored materials, however, little is known about the effects of these materials on composites, particularly newer types such as nanocomposites. Objectives: This study aimed to evaluate the surface microhardness of two types of composites, micro-hybrid (point 4) and nanohybrid (premise), after exposure to natural and industrial juices. Methods: In this experimental study, 90 disc-shaped specimens with a thickness of 2 mm and a diameter of 10 mm were taken from two composites, micro-hybrid Point4 (Kerr) and nanohybrid premise (kerr) (two groups of 45). Then, the specimens of each group were divided into 5 subgroups of 9 and were immersed for 7 days for 6 hours in 5 solutions of industrial orange juice, natural orange juice, industrial pomegranate juice, natural pomegranate juice, and distilled water (control group). Surface microhardness of specimens was measured by Vickers device at baseline, one day and one week after immersing. Data was measured by ANOVA, repeated measure test, and independent t-test. A significant level of α was 0.05. Results: The surface microhardness of two types of composites exposed to beverages was reduced significantly. However, no significant difference was found between natural and industrial juices in none of the composites. Conclusions: Natural and industrial juices can affect the surface microhardness of composites, which varies depending on the type of composite and the type of juice and immersion time.","PeriodicalId":53873,"journal":{"name":"Journal of Dentistry Indonesia","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47915632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adailton de Morais Cavalcante, Renata Cordeiro Teixeira Medeiros, Danilo Lopes Ferreira Lima, S. Santos
The rehabilitation of complex cases in the anterior region of the maxilla may require knowledge of several dental specialties. when dental involvement also causes bone and gingival involvement, management usually requires adjustment of gingival zenitis and bone graft to compensate for bone loss. Objective: to discuss a case of previous orthodontic treatment for implant-prosthetic rehabilitation in the anterior region. Case report: This case report describes a 40-year-old patient who came with the intention of extracting the tooth and installing a bridge. The implant-prosthetic treatment was performed after previous orthodontic treatment to adapt the gingiva and space. Conclusion: The interrelationship of specialists in oral rehabilitation improved the aesthetic and functional result of this complex case. Orthodontic mechanics and periodontics adapted the space and improved the periodontal condition before the implant was installed, resulting in a very satisfactory aesthetic, improving the patient’s selfesteem.
{"title":"Interrelationship Between Orthodontics and Implantology in Anterior Aesthetic Rehabilitation: A Case Report","authors":"Adailton de Morais Cavalcante, Renata Cordeiro Teixeira Medeiros, Danilo Lopes Ferreira Lima, S. Santos","doi":"10.14693/JDI.V28I2.1153","DOIUrl":"https://doi.org/10.14693/JDI.V28I2.1153","url":null,"abstract":"The rehabilitation of complex cases in the anterior region of the maxilla may require knowledge of several dental specialties. when dental involvement also causes bone and gingival involvement, management usually requires adjustment of gingival zenitis and bone graft to compensate for bone loss. Objective: to discuss a case of previous orthodontic treatment for implant-prosthetic rehabilitation in the anterior region. Case report: This case report describes a 40-year-old patient who came with the intention of extracting the tooth and installing a bridge. The implant-prosthetic treatment was performed after previous orthodontic treatment to adapt the gingiva and space. Conclusion: The interrelationship of specialists in oral rehabilitation improved the aesthetic and functional result of this complex case. Orthodontic mechanics and periodontics adapted the space and improved the periodontal condition before the implant was installed, resulting in a very satisfactory aesthetic, improving the patient’s selfesteem.","PeriodicalId":53873,"journal":{"name":"Journal of Dentistry Indonesia","volume":"28 1","pages":"112-117"},"PeriodicalIF":0.4,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44132334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Holistic Management F Holistic Management For Severe Oral Lichen Planus: A Case al Lichen Planus: A Case Report","authors":"N. M. Rosdy, Nyi Mas Siti Purwaningsih","doi":"10.14693/JDI.V28I2.1279","DOIUrl":"https://doi.org/10.14693/JDI.V28I2.1279","url":null,"abstract":"","PeriodicalId":53873,"journal":{"name":"Journal of Dentistry Indonesia","volume":"28 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43856956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Calvin Kurnia, Jane Amelia V. Wibisono, D. D. S. Sumantri
The limitation of residual bone height (RBH) and vital structures such as sinuses in the maxillary often make the implant placement procedure becomes complicated. Clinicians may perform sinus elevation prior to implant placement to accommodate the length of the implant fixture. Sinus elevation is an invasive procedure and complication may occur during the surgery including the most frequent complication is perforation of Schneiderian membrane. Objectives: To discuss a comprehensive management of implant placement and its complication step by step from surgical procedures to crown placement Case Report: 67-year-old female patient with partial edentulism in the right maxillary region, presence of sinus septum, and RBH was 4mm. The patient was planned to do sinus elevation surgery prior to implant placement, perforation of the Schneiderian membrane occurred while surgery performed and pericardium membrane was attached around the perforation site. Sinus re-entry and implant placement were performed after 3 months followed by prosthetics procedures in the next 6 month. Conclusion: Dental implant is a complicated treatment and complication may occur during the placement, thus a comprehensive management is very essential to minimize the risk of complications.
{"title":"Maxillary Implant Placement in a Limited Residual Bone Height","authors":"Calvin Kurnia, Jane Amelia V. Wibisono, D. D. S. Sumantri","doi":"10.14693/jdi.v28i2.1254","DOIUrl":"https://doi.org/10.14693/jdi.v28i2.1254","url":null,"abstract":"The limitation of residual bone height (RBH) and vital structures such as sinuses in the maxillary often make the implant placement procedure becomes complicated. Clinicians may perform sinus elevation prior to implant placement to accommodate the length of the implant fixture. Sinus elevation is an invasive procedure and complication may occur during the surgery including the most frequent complication is perforation of Schneiderian membrane. Objectives: To discuss a comprehensive management of implant placement and its complication step by step from surgical procedures to crown placement Case Report: 67-year-old female patient with partial edentulism in the right maxillary region, presence of sinus septum, and RBH was 4mm. The patient was planned to do sinus elevation surgery prior to implant placement, perforation of the Schneiderian membrane occurred while surgery performed and pericardium membrane was attached around the perforation site. Sinus re-entry and implant placement were performed after 3 months followed by prosthetics procedures in the next 6 month. Conclusion: Dental implant is a complicated treatment and complication may occur during the placement, thus a comprehensive management is very essential to minimize the risk of complications.","PeriodicalId":53873,"journal":{"name":"Journal of Dentistry Indonesia","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45742239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Karaman, Saadet Çınarsoy Ciğerim, Nourtzan Kechagia
Objectives: This study aims to evaluate the relationship between the morphological variations of Sella Turcica (ST) and dental anomalies. Methods: This study included 765 individuals between the ages of 13 and 35. ST morphology was examined in 3 groups as no calcification, partially calcified and completely calcified according to the interclinoid ligament (ICL) on lateral cephalometric films, and it was examined and evaluated in terms of its relationship to impacted canines (unilateral and bilateral), impacted teeth (except 3rd molars), root dilaceration, tooth deficiency (upper incisor lateral - lower and upper second premolar), supernumerary tooth and taurodontism. Results: The rates of supernumerary teeth, lateral and premolar tooth deficiency and root dilaceration in total calcification of ICL were significantly higher than those in ICL with no calcification. The rates of taurodontism, unilateral and bilateral impacted canine teeth and impacted teeth in ICL with no calcification were found to be significantly lower than those in the partial and total calcification of ICL. Conclusion: In this study, a statistically significant relationship was found between ST morphology and dental anomalies. Examination of sella turcica bridging (STB) variations in a large population will be guiding for the early detection of dental anomalies.
{"title":"Evaluation of the Relationship between Sella Turcica Bridging and Dental Anomalies","authors":"A. Karaman, Saadet Çınarsoy Ciğerim, Nourtzan Kechagia","doi":"10.14693/jdi.v28i2.1251","DOIUrl":"https://doi.org/10.14693/jdi.v28i2.1251","url":null,"abstract":"Objectives: This study aims to evaluate the relationship between the morphological variations of Sella Turcica (ST) and dental anomalies. Methods: This study included 765 individuals between the ages of 13 and 35. ST morphology was examined in 3 groups as no calcification, partially calcified and completely calcified according to the interclinoid ligament (ICL) on lateral cephalometric films, and it was examined and evaluated in terms of its relationship to impacted canines (unilateral and bilateral), impacted teeth (except 3rd molars), root dilaceration, tooth deficiency (upper incisor lateral - lower and upper second premolar), supernumerary tooth and taurodontism. Results: The rates of supernumerary teeth, lateral and premolar tooth deficiency and root dilaceration in total calcification of ICL were significantly higher than those in ICL with no calcification. The rates of taurodontism, unilateral and bilateral impacted canine teeth and impacted teeth in ICL with no calcification were found to be significantly lower than those in the partial and total calcification of ICL. Conclusion: In this study, a statistically significant relationship was found between ST morphology and dental anomalies. Examination of sella turcica bridging (STB) variations in a large population will be guiding for the early detection of dental anomalies.","PeriodicalId":53873,"journal":{"name":"Journal of Dentistry Indonesia","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45091768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chee Zi Yun, N. Nor, Zurairah Berahim, K. Ponnuraj
Kelulut honey or stingless bee honey is a type of honey produced by stingless bees of the Trigona species where the nest is found in living trees. Objective: The aim of this study was to evaluate the cytotoxic potential of Malaysian Kelulut honey by employing MTT assay on a human gingival fibroblast cell line. Methods: Human gingival fibroblast cell line was cultured in minimal essential medium alpha (α-MEM) with 10% foetal bovine serum and 1% penicillin-streptomycin solution in a 5% CO2 incubator at 37°C in a humidified atmosphere. The cells were seeded at a cell density of 5x103 cells/well in a 96-well culture plate for 24 hours. The cells were treated with seven different concentrations (200, 100, 50, 25, 12.5, 6.25, and 3.125mg/ml) of Malaysian Kelulut honey and incubated in a CO2 incubator. The negative control comprised cells treated with growth media alone. The cell viability was assessed using MTT assay at 24, 48, and 72 hours. The test plate was shaken using a microplate shaker and the absorbance of the solution was measured at 570nm using an ELISA reader with the Magellan software. Statistical analysis of the data was carried out using Kruskal-Wallis test and SPSS 24.0.0 for Windows. A p value <0.05 was considered as statistically significant. Results: There was no cytotoxic effect of Malaysian Kelulut honey on HGF-1 based on the MTT assay at different concentrations and at different time points tested as the cell viability was above 70%. The highest percentage of cell viability at all three different durations of treatment were observed at 3.125mg/ml, whereas the lowest cell viability was observed at 200mg/ml of Kelulut honey concentration. However, statistically significant differences were seen between some of the concentrations at various time points. Conclusion: Since the cell viability of HGF-1 treated with Malaysian Kelulut honey was more than 70% at all concentrations ranging from 3.125mg/ml to 200mg/ml at three different time points (24, 48 and 72 hours), Malaysian Kelulut honey can be considered as non-cytotoxic on human gingival fibroblasts based on MTT assay under the present test conditions
Kelulut蜂蜜或无刺蜜蜂蜂蜜是一种由Trigona物种的无刺蜜蜂生产的蜂蜜，在活树上可以找到巢穴。目的：采用MTT法对人牙龈成纤维细胞系进行马来西亚克鲁鲁蜂蜜的细胞毒性研究。方法：人牙龈成纤维细胞系在含有10%胎牛血清和1%青霉素-链霉素溶液的最低必需培养基α（α-MEM）中，在5%CO2培养箱中，在37°C的湿润气氛中培养。将细胞以5x103细胞/孔的细胞密度接种在96孔培养板中24小时。用七种不同浓度（200、100、50、25、12.5、6.25和3.125mg/ml）的马来西亚Keulut蜂蜜处理细胞，并在CO2培养箱中孵育。阴性对照包括单独用生长培养基处理的细胞。在24、48和72小时使用MTT测定法评估细胞活力。使用微孔板振荡器振荡测试板，并使用带有Magellan软件的ELISA读取器在570nm处测量溶液的吸光度。使用Kruskal-Wallis检验和SPSS 24.0.0 for Windows对数据进行统计分析。p值<0.05被认为具有统计学意义。结果：不同浓度、不同时间点的MTT法检测，马来西亚克鲁鲁特蜂蜜对HGF-1无细胞毒性作用，细胞活力均在70%以上。在所有三个不同的处理持续时间内，3.125mg/ml的细胞活力百分比最高，而200mg/ml的克鲁鲁蜂蜜浓度的细胞活力最低。然而，在不同时间点的一些浓度之间存在统计学上的显著差异。结论：马来西亚克鲁鲁特蜂蜜在3.125mg/ml～200mg/ml的浓度范围内，在3个不同时间点（24、48和72小时）对人牙龈成纤维细胞生长因子-1的细胞活力均大于70%，因此在本试验条件下，MTT法可认为马来西亚克鲁特蜂蜜对人牙龈纤维细胞无细胞毒性
{"title":"Cytotoxic Evaluation of Mala aluation of Malaysian K ysian Kelulut Hone elulut Honey on Human y on Human Gingival Fibr al Fibroblast Cell Line using M oblast Cell Line using MTT Assay","authors":"Chee Zi Yun, N. Nor, Zurairah Berahim, K. Ponnuraj","doi":"10.14693/jdi.v28i2.1244","DOIUrl":"https://doi.org/10.14693/jdi.v28i2.1244","url":null,"abstract":"Kelulut honey or stingless bee honey is a type of honey produced by stingless bees of the Trigona species where the nest is found in living trees. Objective: The aim of this study was to evaluate the cytotoxic potential of Malaysian Kelulut honey by employing MTT assay on a human gingival fibroblast cell line. Methods: Human gingival fibroblast cell line was cultured in minimal essential medium alpha (α-MEM) with 10% foetal bovine serum and 1% penicillin-streptomycin solution in a 5% CO2 incubator at 37°C in a humidified atmosphere. The cells were seeded at a cell density of 5x103 cells/well in a 96-well culture plate for 24 hours. The cells were treated with seven different concentrations (200, 100, 50, 25, 12.5, 6.25, and 3.125mg/ml) of Malaysian Kelulut honey and incubated in a CO2 incubator. The negative control comprised cells treated with growth media alone. The cell viability was assessed using MTT assay at 24, 48, and 72 hours. The test plate was shaken using a microplate shaker and the absorbance of the solution was measured at 570nm using an ELISA reader with the Magellan software. Statistical analysis of the data was carried out using Kruskal-Wallis test and SPSS 24.0.0 for Windows. A p value <0.05 was considered as statistically significant. Results: There was no cytotoxic effect of Malaysian Kelulut honey on HGF-1 based on the MTT assay at different concentrations and at different time points tested as the cell viability was above 70%. The highest percentage of cell viability at all three different durations of treatment were observed at 3.125mg/ml, whereas the lowest cell viability was observed at 200mg/ml of Kelulut honey concentration. However, statistically significant differences were seen between some of the concentrations at various time points. Conclusion: Since the cell viability of HGF-1 treated with Malaysian Kelulut honey was more than 70% at all concentrations ranging from 3.125mg/ml to 200mg/ml at three different time points (24, 48 and 72 hours), Malaysian Kelulut honey can be considered as non-cytotoxic on human gingival fibroblasts based on MTT assay under the present test conditions","PeriodicalId":53873,"journal":{"name":"Journal of Dentistry Indonesia","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44693058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eleena Mohd Yusof, Muhamad Amir Hazim Razli, Siti Aisyah Iman Mohd Nasir
{"title":"Assessment of the Working Posture among Dental Students to Prevent Musculosk ent Musculoskeletal Disor eletal Disorders","authors":"Eleena Mohd Yusof, Muhamad Amir Hazim Razli, Siti Aisyah Iman Mohd Nasir","doi":"10.14693/JDI.V28I2.1256","DOIUrl":"https://doi.org/10.14693/JDI.V28I2.1256","url":null,"abstract":"","PeriodicalId":53873,"journal":{"name":"Journal of Dentistry Indonesia","volume":"28 1","pages":"105-111"},"PeriodicalIF":0.4,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46561186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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