Romanian Journal of Morphology and Embryology最新文献

Green tea, derived from Camellia sinensis, contains polyphenolic active compounds that exhibit diverse pharmacological effects including anticancer, anti-inflammatory, antioxidant, and immunomodulatory properties. Employing various concentrations of green tea polyphenols (GTPs; 0, 100, 200, 300, 400, 500 μg∕mL), human normal lung epithelial cells (BEAS-2B) and non-small cell lung cancer (NSCLC) cells (A549) underwent treatment. The cell viability was assessed using the cell counting kit-8 (CCK-8) assay, proliferation was examined through the colony formation assay, apoptosis was monitored via flow cytometry, cell migration, and epithelial-mesenchymal transition (EMT)-associated proteins (E-cadherin, N-cadherin) were determined by Western blot. A549 cells were subjected to Cisplatin (0, 0.5, 1, 1.5 μM) and X-ray irradiation (0, 2, 4, 6 Gy) for treatment to probe the influence of GTPs on A549 cells in response to chemoradiotherapy. The functioning mechanism of GTPs in the context of NSCLC was validated using lithium chloride (LiCl) [a glycogen synthase kinase-3 beta (GSK-3β) inhibitor], which activates the Wnt∕β-catenin pathway. GTPs suppressed NSCLC cell viability in a concentration-dependent pattern, with a half-maximal inhibitory concentration (IC50) of 362.5 μg∕mL, while showing little impact on BEAS-2B cells' viability (at concentrations not exceeding 500 μg∕mL). Treatment with GTPs dampened colony formation of NSCLC cells, while promoting apoptosis. LiCl treatment vigorously attenuated the inhibitory impact of GTPs on the malignant phenotype of NSCLC cells. Mechanistic studies suggested that GTPs strengthened GSK-3β stability, thereby impeding the Wnt∕β-catenin pathway. Tea polyphenols (TPs) in conjunction with concurrent radiochemotherapy (CRCT) enhance the stability of GSK-3β and dampen the Wnt∕β-catenin pathway, hence exerting anticancer effects in NSCLC.

We studied the influence of maternal hypothyroidism on progeny skin morphogenesis by means of histological, histochemical and lectin-histochemical methods. Hypothyroid conditions in rats were achieved by daily food supplementation with antithyroid drug Mercazolil. The experiment was conducted on 10 control and 10 hypothyroid rats, which delivered 70 and 46 offsprings, respectively. We discovered that maternal hypothyroidism induces the accumulation of mast cells (MCs) in the skin of progeny on the 1st, 10th and 20th postnatal days, with decrease of these cell's count returning to control level on 40th postnatal day. These results indicate that offsprings developing under conditions of maternal hypothyroidism are a risk group for changes in immune status and the occurrence of allergic reactions. The stratum corneum of epidermis, its lipid barrier as well as pilosebaceous units, in both control and experimental group animals, at the early stages of postnatal ontogenesis are enriched with carbohydrate determinants of αDMan, βDGal, βDGal(1-3)DGalNAc, αLFuc, αDGalNAc, αDGlcNAc, Neu5Ac. Galanthus nivalis agglutinin (GNA) is a selective histochemical marker of MCs, while Lactarius torminosus fungus agglutinin (LTFA) is a selective label of Langerhans cells. Maternal hypothyroidism resulted in reduction of lectin binding with the structural components of progeny skin and its derivatives. We speculate that alterations in glycoconjugate processing and degradation sequences have an impact on the cell signaling, formation of adhesive contacts, cellular proliferation and differentiation. The lectin set we used clearly demonstrated specific labeling of cellular subpopulations, monitoring glycoconjugates processing and degradation under physiological and pathological conditions in all skin components.

Introduction: The incidence of renal tumors increased substantially in the past decades, being responsible for 3% of deaths caused by cancer globally. They constitute a diverse group with varying morphological, immunohistochemical, and molecular characteristics. The severity and prognosis are directly correlated with tumor stage, histopathological aspect, sarcomatoid/rhabdoid features, fat or vascular invasion.

Aim: The aim of the study was to assess the prevalence trend, as well as demographic and pathological characteristics of renal tumors in Mureş County Clinical Hospital, Romania, and border areas.

Patients, materials and methods: In this retrospective study, all patients who underwent a total or partial nephrectomy for renal tumors were analyzed over a five-year period. The data was collected from the Pathology Department of Mureş County Clinical Hospital.

Results: Among 220 patients, 138 (62.72%) were males and 82 (37.27%) females. The mean age was 62.63 years (range: 33-84 years). The majority of the tumors found were malignant: 204 (92.72%) vs 16 (7.27%) benign. The most common subtype within malignant tumors was clear cell renal cell carcinoma (CCRCC) (n=181), 13.25% cases with sarcomatoid/rhabdoid features. pT3a stage was frequently identified. Vascular invasion and necrosis were observed in a large number of cases, especially for CCRCC with sarcomatoid/rhabdoid features.

Conclusions: The spectrum of adult renal tumors in our region aligns with the data reported in the literature. CCRCC was the most frequent histological subtype and pT3a the most common stage identified. Our study contributes to the understanding of renal tumors characteristics, identifying potential factors with impact in the progression and prognosis of the disease.

Introduction: The accurate grading of cutaneous squamous cell carcinoma (cSCC) is crucial for effective treatment and patient care, but the current method, relying on pathologist visual assessment, can be subjective.

Aim: This study aimed to quantitatively investigate nuclear morphometrics in cSCC tumor cells, specifically examining potential differences between established histological grades and exploring the utility of digital morphometrics in characterizing nuclear morphology.

Materials and methods: We analyzed 47 cSCC cases, utilizing whole slide images and CellProfiler software to extract nuclear morphometric features from a total of 519 828 nuclei.

Results: The results showed significant differences in nuclear size, shape, and spatial arrangement between different tumor grades. Notably, grade 3 tumors exhibited distinct characteristics compared to grades 1 and 2, suggesting a potential shift towards a simplified, 2-tier grading system.

Conclusions: The study highlights the potential of digital morphometrics as a valuable tool for enhancing the accuracy and objectivity of cSCC grading, ultimately improving patient care.

Nonimmune hydrops fetalis (NIHF) is a condition that can occur at any stage of pregnancy and is linked to a variety of disorders, resulting in severe perinatal complications and increased mortality risk. NIHF refers specifically to cases where maternal antibodies against red blood cell antigens are excluded. Early identification of the underlying causes is essential for managing pregnancy and deciding on treatment. The prognosis depends on the causes, both in the short and long term. Each case should be considered individually. We describe a case of severe fetal hydrops first identified at 35 weeks of gestation. In this instance, a definitive diagnosis could not be reached during pregnancy. An accurate diagnosis combined with early prenatal detection of NIHF is vital for reducing neonatal mortality associated with this condition.

The paper provides an overview of the current understanding of different cells and structures' biology [e.g., blood-retinal barrier, Bruch membrane, Clara (club) cells, brush cells and tuft cells, Merkel cells, Hofbauer cells, cytokeratins], including their origin, structure, function, and role in disease pathogenesis, and of the latest findings in the medical literature concerning the paracortex of the lymph nodes.

This paper represents a retrospective study on 252 patients with acute ischemic stroke (AIS), who received thrombolytic therapy between 2019-2023, in the Department of Neurology at the Emergency County Hospital in Craiova, Romania. We aimed to evaluate treatment complications and short-term outcomes of patients with AIS, treated with intravenous (i.v.) recombinant tissue plasminogen activator (rtPA; Alteplase), within 4.5 hours from onset. We also tried to assess the correlations between classical risk factors, such as diabetes, hypertension, atrial fibrillation, smoking and the outcome of these patients. Two hundred and fifty-two patients were treated with i.v. thrombolysis in our Department between October 2019 and December 2023. Out of the total thrombolysed patients, 146 were men and 106 were women. Our focus was on the clinical evolution of our patients and on the complications, especially on hemorrhagic transformation and death, and we tried to see if there were any correlations between gender, age, vascular risk factors such as diabetes, smoke, obesity, hypertension, atrial fibrillation and the outcome of our thrombolysed patients. Out of the total of 252 thrombolysed patients, only 55 (21.83%) had hemorrhagic transformation. Analyzing the differences between the occurrence of hemorrhagic transformation, respectively death, depending on the location and type of stroke, we did not identify statistically significant differences. The only comparison where a difference close to the significance limit is observed is that related to the frequency of deaths depending on the type of stroke - patients with cardioembolic stroke had a higher death rate.

Lower third molars are frequently extracted due to pathologies of the dental follicle, pericoronitis, advanced carious lesions, orthodontic reasons (risk of anterior tooth crowding) or causing periodontal or carious lesions in the distal area of the second molar. The case presented here is of a male patient that came to our Clinic experiencing pain in the distal area of one of his old bridges. The clinical examination revealed a malpositioned, but unusually functional third molar; it is a very rare situation for an initially impacted third molar to erupt in an almost horizontal position and not only to remain on the arch for a very long period of time, but also to contribute to mastication efficiency and occlusion, despite the fact that masticatory forces are distributed at a right angle on its long axis and that mastication takes place on the distal surface of the crown and root, and not on the usually occlusal cusped surface. The horizontal mandibular right third molar contributed to maintaining the vertical dimension of occlusion and the masticatory efficiency for a very long period of time; it also ensured a proper distribution of forces trough the long axis of the second premolar, since the distal contact of the third's molar crown with the second premolar helped it to resist masticatory forces and to remain on the arch, despite the prolonged absence of any mesial contact.

The association between different viruses and cancer is well acknowledged in human pathology. However, the precise understanding of how oncogenic viruses intervene in the development of cancer of salivary glands (SGs) remains incomplete. Our review aimed to explore the current literature regarding SGs cancer to evaluate the potential involvement of human papillomaviruses (HPVs) in their development. Our work hypothesis suggests that viruses may be involved in these tumors' immunogenicity. The literature analysis showed HPV-positive detection in different head and neck malignancies, added to variable risk factors and a constant increasing incidence rate. HPV tumorigenesis mechanism is complex, involving E6 and E7 HPV oncoproteins. Additionally, the reported findings generally support the relationship between SGs cancer and high oncogenic subtypes 16 and 18. However, the pathogenic contribution of HPV subtypes 11, 33, 45, 52, 56, and 59 is controversial and further research may clarify their potential involvement in the SG tumor's development. This literature review may enhance the understanding of the role of viral contribution as an etiopathogenic co-factor in SG carcinogenesis. Thus, these data may add value to innovative concepts and treatment strategies development, given that virus-induced tumors can be prevented by targeted immunization, added to oncolytic or viral therapy.

Angiosarcoma represents a group of rare tumors originating from vascular and lymphatic endothelial cells, characterized by marked aggressiveness, rapid growth and poor clinical outcome. The incidence of breast angiosarcoma accounts for approximately 0.05% of all malignant breast tumors and less than 1% of all sarcomas. In this article, we report the case of a 67-year-old female patient who presented to our Clinic due to a rapidly evolving, non-painful, vegetating mass, encompassing almost her entire left breast. Imaging studies revealed diffuse skin thickening in all quadrants and an intensely opaque axillary lymph node (LN). Interestingly, the patient had prior medical history of breast carcinoma treated conservatively in 2007 with limited breast resection and left axillary lymphadenectomy, followed by post-operative chemotherapy and radiotherapy. At the current presentation, we performed a radical mastectomy with ipsilateral lymphadenectomy. The histopathological examination revealed a secondary angiosarcoma with LN involvement mimicking an atypical vascular lesion. In this article, we report the clinicopathological particularities of this case and discuss the challenge of diagnosing LN involvement in angiosarcoma.