Metastasis is a major adverse prognostic factors of colon cancer. Aspirin chemoprophylaxis may improve outcomes for metastatic colon cancer patients. This study aimed to determine the impact of metastasis-related molecular subtypes on prognosis and aspirin chemoprophylaxis benefit.
Methods
We obtained differentially expressed metastasis-related genes in cancer and normal tissues. A weighted gene co-expression network (WGCNA) was constructed by differentially expressed genes. Lasso-Cox regression identified key prognostic genes within relevant modules, establishing a risk score model. Transcription factors regulating module genes were explored. Aspirin-interacting genes were identified using the Comparative Toxicogenomics Database (CTD) and validated via cellular experiments.
Results
WGCNA analysis of 2062 metastasis-related genes revealed significant correlations between blue/yellow modules and colon cancer. A risk score model based on blue module genes predicted overall survival and 1-, 3-, and 5-year survival rates. Transcription factor analysis implicated the E2F family in blue module regulation and NFκB1/STAT3 in yellow module regulation. CTD analysis showed persistent upregulation of NOX4, CXCL8, CXCL5, GDF15, and MMP13 post-aspirin treatment. Cellular experiments confirmed aspirin downregulated metastasis-related genes (E2F1, CCNE1, VEGFA, MMP3) in colon cancer.
Conclusion
We developed a validated metastasis gene predictive model. Colon cancer patients with upregulated NOX4, CXCL8, CXCL5, GDF15, or MMP13 may not benefit from aspirin chemoprophylaxis. Conversely, patients showing aspirin-induced downregulation of E2F1, CCNE1, VEGFA, and MMP3 may derive chemoprophylactic benefit.
{"title":"Risk Prediction of Colon Cancer Metastasis and Bioinformatics Analysis of Aspirin Treatment","authors":"Jing Li, Xinyue Yu, Jingjing Shao, Hui Zhang, Jiawei Yu","doi":"10.1155/ijog/8412254","DOIUrl":"10.1155/ijog/8412254","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Metastasis is a major adverse prognostic factors of colon cancer. Aspirin chemoprophylaxis may improve outcomes for metastatic colon cancer patients. This study aimed to determine the impact of metastasis-related molecular subtypes on prognosis and aspirin chemoprophylaxis benefit.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We obtained differentially expressed metastasis-related genes in cancer and normal tissues. A weighted gene co-expression network (WGCNA) was constructed by differentially expressed genes. Lasso-Cox regression identified key prognostic genes within relevant modules, establishing a risk score model. Transcription factors regulating module genes were explored. Aspirin-interacting genes were identified using the Comparative Toxicogenomics Database (CTD) and validated via cellular experiments.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>WGCNA analysis of 2062 metastasis-related genes revealed significant correlations between blue/yellow modules and colon cancer. A risk score model based on blue module genes predicted overall survival and 1-, 3-, and 5-year survival rates. Transcription factor analysis implicated the E2F family in blue module regulation and NF<i>κ</i>B1/STAT3 in yellow module regulation. CTD analysis showed persistent upregulation of NOX4, CXCL8, CXCL5, GDF15, and MMP13 post-aspirin treatment. Cellular experiments confirmed aspirin downregulated metastasis-related genes (E2F1, CCNE1, VEGFA, MMP3) in colon cancer.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>We developed a validated metastasis gene predictive model. Colon cancer patients with upregulated NOX4, CXCL8, CXCL5, GDF15, or MMP13 may not benefit from aspirin chemoprophylaxis. Conversely, patients showing aspirin-induced downregulation of E2F1, CCNE1, VEGFA, and MMP3 may derive chemoprophylactic benefit.</p>\u0000 </section>\u0000 </div>","PeriodicalId":55239,"journal":{"name":"Comparative and Functional Genomics","volume":"2025 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12714093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145804336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hepatocellular carcinoma (HCC) is a major global health challenge, characterized by complex molecular mechanisms. This review focuses on the crucial roles of microRNAs (miRNAs) in HCC development, progression, and therapeutic response. The regulation of gene expression and several critical cellular processes is carried out by miRNAs. These small, noncoding RNAs play a significant role in apoptosis, DNA repair, immune regulation, angiogenesis, cell migration, invasion, and tumor progression. MiRNAs have been identified as valuable noninvasive biomarkers, which suggests their potential use in early diagnosis, prognosis, and tracking the effectiveness of treatments. The relationship between single nucleotide polymorphisms (SNPs) in miRNA binding sites and their impact on both the vulnerability to and the development of HCC is also a topic of this discussion. These genetic variations can alter miRNA-mRNA interactions, affecting the expression of critical genes involved in HCC, which modulates key cellular processes such as apoptosis, DNA repair, and immune regulation. Emerging technologies like liquid biopsies and exosomal miRNA analysis are explored for their potential to revolutionize HCC diagnosis and treatment. This first-of-its-kind comprehensive review consolidates current findings on miRNA-SNP interactions across four major HCC pathogenic pillars (apoptosis, DNA repair, immune evasion, and metastasis), providing novel, noninvasive genetic biomarkers for HCC risk stratification, prognosis prediction, and tailoring individualized therapeutic regimens.
{"title":"MicroRNA Binding Site Polymorphisms in Hepatocellular Carcinoma: Implications for Pathogenesis, Prognosis, and Therapeutic Response","authors":"Sara Rezapour, Farin Khodam Mohammadi, Mahmoud Darweesh, Saeed Mohammadi, Saeid Afshar","doi":"10.1155/ijog/9520856","DOIUrl":"10.1155/ijog/9520856","url":null,"abstract":"<p>Hepatocellular carcinoma (HCC) is a major global health challenge, characterized by complex molecular mechanisms. This review focuses on the crucial roles of microRNAs (miRNAs) in HCC development, progression, and therapeutic response. The regulation of gene expression and several critical cellular processes is carried out by miRNAs. These small, noncoding RNAs play a significant role in apoptosis, DNA repair, immune regulation, angiogenesis, cell migration, invasion, and tumor progression. MiRNAs have been identified as valuable noninvasive biomarkers, which suggests their potential use in early diagnosis, prognosis, and tracking the effectiveness of treatments. The relationship between single nucleotide polymorphisms (SNPs) in miRNA binding sites and their impact on both the vulnerability to and the development of HCC is also a topic of this discussion. These genetic variations can alter miRNA-mRNA interactions, affecting the expression of critical genes involved in HCC, which modulates key cellular processes such as apoptosis, DNA repair, and immune regulation. Emerging technologies like liquid biopsies and exosomal miRNA analysis are explored for their potential to revolutionize HCC diagnosis and treatment. This first-of-its-kind comprehensive review consolidates current findings on miRNA-SNP interactions across four major HCC pathogenic pillars (apoptosis, DNA repair, immune evasion, and metastasis), providing novel, noninvasive genetic biomarkers for HCC risk stratification, prognosis prediction, and tailoring individualized therapeutic regimens.</p>","PeriodicalId":55239,"journal":{"name":"Comparative and Functional Genomics","volume":"2025 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12705504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145774383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ying Fang, Jifang Peng, Dandan Yang, Hairong Liu, Tianle Chen, Juan Cao
<div> <section> <h3> Background</h3> <p>The purpose of the study is to evaluate the comparative effects of conventional perioperative care and conventional care plus Traditional Chinese Medicine (TCM) appropriate technology on blood glucose fluctuation, comfort level, and stress response in postoperative esophageal cancer patients.</p> </section> <section> <h3> Methods</h3> <p>This study included 100 patients who underwent radical esophagectomy in our hospital’s thoracic surgery department from January 2023 to June 2024. Patients were randomly divided into a study group and a control group, with 50 cases in each group, using a computer-generated random number table (generated via SPSS 22.0 software) to ensure randomization integrity. The control group received conventional perioperative care, while the study group received TCM appropriate technology in addition to conventional care. Blood glucose fluctuations were monitored 1 day before surgery, and on postoperative Days 1, 3, 7, and 14. On the day before surgery and postoperative Day 14, the General Comfort Questionnaire (GCQ) and Perceived Nursing Service Quality (PNSQ) scale were used to evaluate patients’ comfort levels and perception of service quality. The Connor–Davidson Resilience Scale (CD-RISC) was used to assess psychological stress state. Physiological stress was evaluated by analyzing adrenocorticotropic hormone (ACTH) and cortisol (Cor) levels.</p> </section> <section> <h3> Results</h3> <p>Significant differences in blood glucose fluctuation values were observed between the study group and control group (between-group F = 9.457, <i>p</i> < 0.001). Significant differences also existed across different time points (time point F = 6.542, <i>p</i> < 0.001), with a significant interaction effect between group and time point (group∗time point F = 10.055, <i>p</i> < 0.001). Although blood glucose fluctuation levels in both groups showed a downward trend, the study group demonstrated significantly lower blood glucose fluctuation values than the control group at all time points: 1 day before surgery, and on postoperative days 1, 3, 7, and 14 (<i>p</i> < 0.05). The study group’s total GCQ score on postoperative Day 14 (89.36 ± 12.40) was higher than the control group (73.07 ± 10.14), with all intergroup differences in scores across the four dimensions being statistically significant (<i>p</i> < 0.001). The study group’s CD-RISC score on postoperative Day 14 (74.23 ± 10.3) was higher than the control group (65.34 ± 9.06), while ACTH (12.23 ± 1.68 ng/L) and Cor (9.46 ± 1.29 ng/L) levels were lower than the control group (14.41 ± 1.98 and 10.22 ± 1.41 ng/L, respectively). All differences were st
背景：本研究旨在评价常规围手术期护理与常规护理加中医适宜技术对食管癌术后患者血糖波动、舒适度及应激反应的影响。方法：本研究纳入了2023年1月至2024年6月在我院胸外科行根治性食管切除术的100例患者。将患者随机分为研究组和对照组，每组各50例，采用计算机生成的随机数字表（通过SPSS 22.0软件生成），以保证随机化的完整性。对照组采用常规围手术期护理，研究组在常规护理的基础上采用中医适宜技术。术前1天及术后第1、3、7、14天监测血糖波动。术前和术后第14天分别采用一般舒适度问卷（GCQ）和感知护理服务质量量表（PNSQ）评估患者的舒适度和服务质量感知。采用康诺-戴维森弹性量表（CD-RISC）评估心理应激状态。通过分析促肾上腺皮质激素（ACTH）和皮质醇（Cor）水平来评估生理应激。结果：研究组与对照组血糖波动值有显著差异（组间F = 9.457, p < 0.001）。不同时间点间存在显著差异（时间点F = 6.542, p < 0.001），组与时间点间存在显著交互作用（组*时间点F = 10.055, p < 0.001）。虽然两组患者血糖波动水平均呈下降趋势，但研究组在术前1天、术后1、3、7、14天各时间点血糖波动值均明显低于对照组（p < 0.05）。研究组术后第14天GCQ总分（89.36±12.40）高于对照组（73.07±10.14），4个维度评分组间差异均有统计学意义（p < 0.001）。研究组术后第14天CD-RISC评分（74.23±10.3）高于对照组（65.34±9.06），ACTH（12.23±1.68 ng/L）和Cor（9.46±1.29 ng/L）低于对照组（14.41±1.98和10.22±1.41 ng/L）。差异均有统计学意义（p < 0.001）。结论：中医适宜技术可显著降低根治性食管切除术患者的血糖波动，提高患者的舒适度，并对心理应激（通过较高的康纳-戴维森恢复力量表[CD-RISC]评分：74.23±10.3比65.34±9.06）和生理应激（通过较低的促肾上腺皮质激素[ACTH]: 12.23±1.68比14.41±1.98 ng/L；皮质醇[Cor]: 9.46±1.29比10.22±1.41 ng/L）产生积极影响。
{"title":"Data-Driven Investigation of Traditional Chinese Medicine Approaches for Optimizing Multiple Clinical Parameters in Esophageal Cancer Treatment","authors":"Ying Fang, Jifang Peng, Dandan Yang, Hairong Liu, Tianle Chen, Juan Cao","doi":"10.1155/ijog/6667025","DOIUrl":"10.1155/ijog/6667025","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The purpose of the study is to evaluate the comparative effects of conventional perioperative care and conventional care plus Traditional Chinese Medicine (TCM) appropriate technology on blood glucose fluctuation, comfort level, and stress response in postoperative esophageal cancer patients.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This study included 100 patients who underwent radical esophagectomy in our hospital’s thoracic surgery department from January 2023 to June 2024. Patients were randomly divided into a study group and a control group, with 50 cases in each group, using a computer-generated random number table (generated via SPSS 22.0 software) to ensure randomization integrity. The control group received conventional perioperative care, while the study group received TCM appropriate technology in addition to conventional care. Blood glucose fluctuations were monitored 1 day before surgery, and on postoperative Days 1, 3, 7, and 14. On the day before surgery and postoperative Day 14, the General Comfort Questionnaire (GCQ) and Perceived Nursing Service Quality (PNSQ) scale were used to evaluate patients’ comfort levels and perception of service quality. The Connor–Davidson Resilience Scale (CD-RISC) was used to assess psychological stress state. Physiological stress was evaluated by analyzing adrenocorticotropic hormone (ACTH) and cortisol (Cor) levels.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Significant differences in blood glucose fluctuation values were observed between the study group and control group (between-group F = 9.457, <i>p</i> < 0.001). Significant differences also existed across different time points (time point F = 6.542, <i>p</i> < 0.001), with a significant interaction effect between group and time point (group∗time point F = 10.055, <i>p</i> < 0.001). Although blood glucose fluctuation levels in both groups showed a downward trend, the study group demonstrated significantly lower blood glucose fluctuation values than the control group at all time points: 1 day before surgery, and on postoperative days 1, 3, 7, and 14 (<i>p</i> < 0.05). The study group’s total GCQ score on postoperative Day 14 (89.36 ± 12.40) was higher than the control group (73.07 ± 10.14), with all intergroup differences in scores across the four dimensions being statistically significant (<i>p</i> < 0.001). The study group’s CD-RISC score on postoperative Day 14 (74.23 ± 10.3) was higher than the control group (65.34 ± 9.06), while ACTH (12.23 ± 1.68 ng/L) and Cor (9.46 ± 1.29 ng/L) levels were lower than the control group (14.41 ± 1.98 and 10.22 ± 1.41 ng/L, respectively). All differences were st","PeriodicalId":55239,"journal":{"name":"Comparative and Functional Genomics","volume":"2025 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12703119/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145768004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaoyue Zhou, Cong Fu, Ying Fu, Ting Jiao, Chenyu Zhao, Dan Yang
Background
Lung adenocarcinoma (LUAD) is the most common subtype of lung cancer, where its complex tumor microenvironment (TME) significantly influences disease progression and treatment response. Inflammatory cancer-associated fibroblasts (iCAFs), as a key component of the TME, can promote tumor immune evasion and drug resistance. However, the characteristics of iCAFs in LUAD and their clinical significance have not been fully elucidated.
Methods
The bulk RNA data and scRNA-seq data of LUAD from public databases were integrated to identify and characterize iCAFs subsets and screen their feature genes. iCAF-based signature (ICAFBS) was generated using multiple machine learning algorithms and confirmed in multiple independent cohorts. The relationship between ICAFBS and immune landscape as well as drug sensitivity was further analyzed. Finally, a series of functional studies were conducted to elucidate the role of PFN2 in LUAD cell lines.
Results
The iCAF subtype identified by single-cell analysis was enriched in LUAD and closely linked to poor prognosis. Based on 145 iCAF characteristic genes, ICAFBS was finally screened out four key genes, MGP, LOXL2, FSTL3, and PFN2. ICAFBS demonstrated excellent prognostic predictive capabilities and was validated in multiple external datasets. Patients in the high ICAFBS group showed significant high expression of multiple immune checkpoints. Notably, silencing PFN2 inhibited cell viability and proliferation in LUAD cells, highlighting its potential as a therapeutic target.
Conclusion
As a novel prognostic signature, ICAFBS can effectively predict the clinical outcome, immune landscape and treatment response of patients, providing an important reference for the individualized treatment of LUAD.
{"title":"A Multi-Faceted Approach to Explore the Role of Inflammatory CAFs, Providing Prognostic Value and Therapeutic Implications in Lung Adenocarcinoma","authors":"Xiaoyue Zhou, Cong Fu, Ying Fu, Ting Jiao, Chenyu Zhao, Dan Yang","doi":"10.1155/ijog/6004629","DOIUrl":"10.1155/ijog/6004629","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Lung adenocarcinoma (LUAD) is the most common subtype of lung cancer, where its complex tumor microenvironment (TME) significantly influences disease progression and treatment response. Inflammatory cancer-associated fibroblasts (iCAFs), as a key component of the TME, can promote tumor immune evasion and drug resistance. However, the characteristics of iCAFs in LUAD and their clinical significance have not been fully elucidated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The bulk RNA data and scRNA-seq data of LUAD from public databases were integrated to identify and characterize iCAFs subsets and screen their feature genes. iCAF-based signature (ICAFBS) was generated using multiple machine learning algorithms and confirmed in multiple independent cohorts. The relationship between ICAFBS and immune landscape as well as drug sensitivity was further analyzed. Finally, a series of functional studies were conducted to elucidate the role of PFN2 in LUAD cell lines.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The iCAF subtype identified by single-cell analysis was enriched in LUAD and closely linked to poor prognosis. Based on 145 iCAF characteristic genes, ICAFBS was finally screened out four key genes, MGP, LOXL2, FSTL3, and PFN2. ICAFBS demonstrated excellent prognostic predictive capabilities and was validated in multiple external datasets. Patients in the high ICAFBS group showed significant high expression of multiple immune checkpoints. Notably, silencing PFN2 inhibited cell viability and proliferation in LUAD cells, highlighting its potential as a therapeutic target.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>As a novel prognostic signature, ICAFBS can effectively predict the clinical outcome, immune landscape and treatment response of patients, providing an important reference for the individualized treatment of LUAD.</p>\u0000 </section>\u0000 </div>","PeriodicalId":55239,"journal":{"name":"Comparative and Functional Genomics","volume":"2025 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/ijog/6004629","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145739840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Minqi Chen, Bo Yang, Changming Gong, Xiao Liao, Kunwu He
Background
Despite extensive research, nasopharyngeal carcinoma (NPC) remains a complex malignancy with poorly understood cellular dynamics and risk factors. The relationship between allergic rhinitis and NPC development has been controversial. This study combined single-cell transcriptomics with Mendelian randomization to comprehensively map cellular heterogeneity and establish potential causal links between allergic rhinitis and NPC pathogenesis.
Methods
Researchers performed single-cell RNA sequencing on NPC and adjacent normal tissue samples. Simultaneously, a two-sample Mendelian randomization approach was employed using allergic rhinitis–associated genetic variants as instrumental variables to investigate causality between allergic rhinitis and NPC risk. Integration of these genetic instruments with transcriptomic profiles enabled the identification of genetically influenced molecular pathways.
Results
Comprehensive analysis revealed intricate cellular landscapes comprising epithelial, immune, endothelial, and stromal cell populations, each demonstrating unique transcriptional signatures. Mendelian randomization analysis provided evidence for a causal relationship between allergic rhinitis and NPC development (OR = 1.42, 95% CI: 1.18–1.76, p = 0.0003). Significant dysregulation was observed in critical signaling pathways, including mitochondrial processes, extracellular matrix–receptor interactions, and Wnt/Notch cascades. Genetic instruments for allergic rhinitis showed significant effects on inflammatory pathways within specific NPC cellular subpopulations, suggesting mechanistic links between allergic inflammation and carcinogenesis.
Conclusion
By leveraging both single-cell transcriptomics and Mendelian randomization, this study provides unprecedented insights into NPC′s cellular complexity and establishes causal pathways linking allergic rhinitis to NPC development. These findings identify genetically validated molecular mechanisms that could represent promising therapeutic targets for this challenging malignancy.
背景尽管有广泛的研究，鼻咽癌（NPC）仍然是一种复杂的恶性肿瘤，对细胞动力学和危险因素知之甚少。变应性鼻炎与鼻咽癌发展的关系一直存在争议。本研究将单细胞转录组学与孟德尔随机化相结合，全面绘制细胞异质性，并建立变应性鼻炎与鼻咽癌发病机制之间的潜在因果关系。方法对鼻咽癌及邻近正常组织标本进行单细胞RNA测序。同时，采用双样本孟德尔随机化方法，将变应性鼻炎相关遗传变异作为工具变量，研究变应性鼻炎与鼻咽癌风险之间的因果关系。将这些遗传工具与转录组谱相结合，可以识别受遗传影响的分子途径。结果：综合分析揭示了复杂的细胞景观，包括上皮细胞、免疫细胞、内皮细胞和基质细胞群，每种细胞都表现出独特的转录特征。孟德尔随机化分析为变应性鼻炎与NPC发展之间的因果关系提供了证据（OR = 1.42, 95% CI: 1.18-1.76, p = 0.0003）。在关键信号通路中观察到显著的失调，包括线粒体过程、细胞外基质-受体相互作用和Wnt/Notch级联。变应性鼻炎的遗传工具对特定鼻咽癌细胞亚群的炎症通路有显著影响，提示变应性炎症与癌变之间存在机制联系。通过利用单细胞转录组学和孟德尔随机化，本研究为鼻咽癌的细胞复杂性提供了前所未有的见解，并建立了变应性鼻炎与鼻咽癌发展之间的因果关系。这些发现确定了经过基因验证的分子机制，可以代表这种具有挑战性的恶性肿瘤的有希望的治疗靶点。
{"title":"Machine Learning and Mendelian Randomization Identify Allergic Rhinitis as Nasopharyngeal Carcinoma Risk Factor With Validated Potential Candidate Biomarkers","authors":"Minqi Chen, Bo Yang, Changming Gong, Xiao Liao, Kunwu He","doi":"10.1155/ijog/2281787","DOIUrl":"https://doi.org/10.1155/ijog/2281787","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Despite extensive research, nasopharyngeal carcinoma (NPC) remains a complex malignancy with poorly understood cellular dynamics and risk factors. The relationship between allergic rhinitis and NPC development has been controversial. This study combined single-cell transcriptomics with Mendelian randomization to comprehensively map cellular heterogeneity and establish potential causal links between allergic rhinitis and NPC pathogenesis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Researchers performed single-cell RNA sequencing on NPC and adjacent normal tissue samples. Simultaneously, a two-sample Mendelian randomization approach was employed using allergic rhinitis–associated genetic variants as instrumental variables to investigate causality between allergic rhinitis and NPC risk. Integration of these genetic instruments with transcriptomic profiles enabled the identification of genetically influenced molecular pathways.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Comprehensive analysis revealed intricate cellular landscapes comprising epithelial, immune, endothelial, and stromal cell populations, each demonstrating unique transcriptional signatures. Mendelian randomization analysis provided evidence for a causal relationship between allergic rhinitis and NPC development (OR = 1.42, 95% CI: 1.18–1.76, <i>p</i> = 0.0003). Significant dysregulation was observed in critical signaling pathways, including mitochondrial processes, extracellular matrix–receptor interactions, and Wnt/Notch cascades. Genetic instruments for allergic rhinitis showed significant effects on inflammatory pathways within specific NPC cellular subpopulations, suggesting mechanistic links between allergic inflammation and carcinogenesis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>By leveraging both single-cell transcriptomics and Mendelian randomization, this study provides unprecedented insights into NPC′s cellular complexity and establishes causal pathways linking allergic rhinitis to NPC development. These findings identify genetically validated molecular mechanisms that could represent promising therapeutic targets for this challenging malignancy.</p>\u0000 </section>\u0000 </div>","PeriodicalId":55239,"journal":{"name":"Comparative and Functional Genomics","volume":"2025 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/ijog/2281787","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145739519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The objective was to analyze the effect of C-type lectin domain family 4 member G (CLEC4G) on hepatocellular carcinoma (HCC) and investigate its impact on lenvatinib (Lenva) resistance as well as the underlying action pathway.
Methods
Differentially expressed genes (DEGs) were screened from the GSE101685 dataset, followed by functional enrichment analysis. Subsequently, CLEC4G was selected for subsequent experiments. The Lenva-resistant cell line PLC/PRF/5-R was established and transfected with a CLEC4G silencing expression vector to observe alterations in its biological behavior. Sample size was estimated based on a pilot experiment (n = 3 biological replicates) with 30 clinical samples/cell experiment in each group. Additionally, the expression of the Wnt/β-catenin pathway in PLC/PRF/5-R was examined, and PLC/PRF/5-R activity after intervention with LiCl, a Wnt/β-catenin pathway activator, was evaluated.
Results
A total of 51 DEGs were identified in the GSE101685 dataset. After silencing CLEC4G expression, the activities of both PLC/PRF/5 and PLC/PRF/5-R, as well as the expression of PD-1, were decreased, while apoptosis was increased (p < 0.05). Moreover, silencing CLEC4G inhibited the expression of the Wnt/β-catenin pathway (p < 0.05). After LiCl intervention, the activity of PLC/PRF/5-R was enhanced, and the expression of PD-1 was elevated (p < 0.05). Silencing CLEC4G could reverse the effect of LiCl on PLC/PRF/5-R.
Conclusion
CLEC4G modulates the PD-1 expression of HCC cells through the Wnt/β-catenin pathway, thereby reversing the resistance to Lenva.
{"title":"CLEC4G Reverses Lenvatinib Resistance in Hepatocellular Carcinoma by Suppressing PD-1 Expression via the Wnt/β-Catenin Pathway","authors":"Kemin Xiao, Jin Yan, Guangxi He, Bin He","doi":"10.1155/ijog/9225945","DOIUrl":"10.1155/ijog/9225945","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>The objective was to analyze the effect of <i>C-type lectin domain family 4 member G</i> (<i>CLEC4G</i>) on hepatocellular carcinoma (HCC) and investigate its impact on lenvatinib (Lenva) resistance as well as the underlying action pathway.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Differentially expressed genes (DEGs) were screened from the GSE101685 dataset, followed by functional enrichment analysis. Subsequently, <i>CLEC4G</i> was selected for subsequent experiments. The Lenva-resistant cell line PLC/PRF/5-R was established and transfected with a <i>CLEC4G</i> silencing expression vector to observe alterations in its biological behavior. Sample size was estimated based on a pilot experiment (<i>n</i> = 3 biological replicates) with 30 clinical samples/cell experiment in each group. Additionally, the expression of the Wnt/<i>β</i>-catenin pathway in PLC/PRF/5-R was examined, and PLC/PRF/5-R activity after intervention with LiCl, a Wnt/<i>β</i>-catenin pathway activator, was evaluated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 51 DEGs were identified in the GSE101685 dataset. After silencing CLEC4G expression, the activities of both PLC/PRF/5 and PLC/PRF/5-R, as well as the expression of PD-1, were decreased, while apoptosis was increased (<i>p</i> < 0.05). Moreover, silencing CLEC4G inhibited the expression of the Wnt/<i>β</i>-catenin pathway (<i>p</i> < 0.05). After LiCl intervention, the activity of PLC/PRF/5-R was enhanced, and the expression of PD-1 was elevated (<i>p</i> < 0.05). Silencing CLEC4G could reverse the effect of LiCl on PLC/PRF/5-R.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p><i>CLEC4G</i> modulates the <i>PD-1</i> expression of HCC cells through the Wnt/<i>β</i>-catenin pathway, thereby reversing the resistance to Lenva.</p>\u0000 </section>\u0000 </div>","PeriodicalId":55239,"journal":{"name":"Comparative and Functional Genomics","volume":"2025 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/ijog/9225945","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145739474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Raiyyan Abdur Rahim, Maria Chowdhory, Mahmuda Akhter Hasi, Fabiha Haque, Arif Hasan Khan Robin
LIM genes are essential for cytoskeletal organization and cellular stress responses in plants. This study conducted a comprehensive genome-wide investigation of LIM domain-associated genes in Phaseolus vulgaris. This study expands LIM gene classification beyond traditional domains to include LIM_bind factors, revealing that P. vulgaris has a more complex and functionally diverse regulatory toolkit than previously understood. Seventeen identified PvLIM genes are distributed across three families: LIM-LIM domain, LIM-DA1_like domain, and LIM_bind domain-containing. Following gene duplication, P. vulgaris LIM genes underwent subfunctionalization where ancestral functions were partitioned among copies, creating specialized tissue-specific and stress-responsive roles while maintaining complementary regulatory networks. PvLIM proteins form critical networks controlling cell wall metabolism, signaling pathways, actin cytoskeleton dynamics, and endocytosis. All PvLIM genes exhibit universal responsiveness to multiple stress stimuli including abscisic acid, ethylene, methyl jasmonate, wound, heat, and anaerobic stress conditions, confirming their central role in plant stress tolerance mechanisms. Gene expression analysis revealed tissue-specific functional specialization, with LIM-LIM and LIM_bind domain genes predominantly active in stem and leaf tissues, while PvLIM14 governs stem, pod, and leaf development and PvLIM16 modulates seed-related processes. PvLIM genes constitute essential molecular machinery governing plant growth, development, and stress adaptation through integrated phytohormone-mediated regulatory networks.
{"title":"Genome Wide Identification and Characterization of LIM Domain Associated Gene Superfamily in Common Bean (Phaseolus vulgaris L.)","authors":"Raiyyan Abdur Rahim, Maria Chowdhory, Mahmuda Akhter Hasi, Fabiha Haque, Arif Hasan Khan Robin","doi":"10.1155/ijog/5584901","DOIUrl":"10.1155/ijog/5584901","url":null,"abstract":"<p><i>LIM genes</i> are essential for cytoskeletal organization and cellular stress responses in plants. This study conducted a comprehensive genome-wide investigation of LIM domain-associated genes in <i>Phaseolus vulgaris</i>. This study expands LIM gene classification beyond traditional domains to include LIM_bind factors, revealing that <i>P. vulgaris</i> has a more complex and functionally diverse regulatory toolkit than previously understood. Seventeen identified <i>PvLIM genes</i> are distributed across three families: LIM-LIM domain, LIM-DA1_like domain, and LIM_bind domain-containing. Following gene duplication, <i>P. vulgaris LIM genes</i> underwent subfunctionalization where ancestral functions were partitioned among copies, creating specialized tissue-specific and stress-responsive roles while maintaining complementary regulatory networks. PvLIM proteins form critical networks controlling cell wall metabolism, signaling pathways, actin cytoskeleton dynamics, and endocytosis. All <i>PvLIM genes</i> exhibit universal responsiveness to multiple stress stimuli including abscisic acid, ethylene, methyl jasmonate, wound, heat, and anaerobic stress conditions, confirming their central role in plant stress tolerance mechanisms. Gene expression analysis revealed tissue-specific functional specialization, with LIM-LIM and LIM_bind domain genes predominantly active in stem and leaf tissues, while PvLIM14 governs stem, pod, and leaf development and PvLIM16 modulates seed-related processes. <i>PvLIM genes</i> constitute essential molecular machinery governing plant growth, development, and stress adaptation through integrated phytohormone-mediated regulatory networks.</p>","PeriodicalId":55239,"journal":{"name":"Comparative and Functional Genomics","volume":"2025 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/ijog/5584901","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145739572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The escalating threats of global warming and the increasing demand for sustainable resources have driven research towards identifying resilient organisms capable of thriving under changing environmental conditions. A recently identified strain of Neopyropia yezoensis from Daebudo has demonstrated the ability to grow even under elevated temperatures. Understanding the genetic and molecular mechanisms underlying this resilience is crucial for the development of heat-tolerant cultivars.
Methods
This study investigates the characteristics of the newly isolated strain N. yezoensis (Daebudo) through comprehensive transcriptome analysis on samples cultivated at various temperatures. Transcript reads were aligned to the genome sequences of N. yezoensis (susabi-nori) and Neoporphyra haitanensis. Aligned and unaligned reads were assembled separately, generating 45,089 transcripts and 105,750 transcripts, respectively. The transcripts were annotated using homologous sequences from the Swiss-Prot, NCBI NR, Pfam, SignalP, and KEGG databases. Differentially expressed gene (DEG) analysis was performed, and gene function classification (Gene Ontology) was conducted using BLASTX and BLASTP results from the Swiss-Prot database.
Results
Some transcripts that were upregulated under higher temperatures were found to be involved in key metabolic pathways such as glycolysis or photosynthesis. Also, several DEGs, including heat shock proteins and elongation factor 1 alpha, were identified as potential contributors to high-temperature tolerance. These DEGs are likely involved in cellular stress response and protein synthesis, facilitating growth under elevated temperatures.
Conclusion
The findings provide new molecular-level insights into the growth mechanisms of N. yezoensis under heat stress. This information can be applied to the development of new cultivars with enhanced growth and heat tolerance, supporting sustainable aquaculture in the face of climate change.
{"title":"Genome-Guided and De Novo Transcriptome Analysis of a Newly Isolated Neopyropia yezoensis Strain With Heat Tolerance","authors":"Jong-il Choi, Jiae Kim","doi":"10.1155/ijog/9130170","DOIUrl":"10.1155/ijog/9130170","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The escalating threats of global warming and the increasing demand for sustainable resources have driven research towards identifying resilient organisms capable of thriving under changing environmental conditions. A recently identified strain of <i>Neopyropia yezoensis</i> from Daebudo has demonstrated the ability to grow even under elevated temperatures. Understanding the genetic and molecular mechanisms underlying this resilience is crucial for the development of heat-tolerant cultivars.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This study investigates the characteristics of the newly isolated strain <i>N. yezoensis</i> (Daebudo) through comprehensive transcriptome analysis on samples cultivated at various temperatures. Transcript reads were aligned to the genome sequences of <i>N. yezoensis</i> (susabi-nori) and <i>Neoporphyra haitanensis</i>. Aligned and unaligned reads were assembled separately, generating 45,089 transcripts and 105,750 transcripts, respectively. The transcripts were annotated using homologous sequences from the Swiss-Prot, NCBI NR, Pfam, SignalP, and KEGG databases. Differentially expressed gene (DEG) analysis was performed, and gene function classification (Gene Ontology) was conducted using BLASTX and BLASTP results from the Swiss-Prot database.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Some transcripts that were upregulated under higher temperatures were found to be involved in key metabolic pathways such as glycolysis or photosynthesis. Also, several DEGs, including heat shock proteins and elongation factor 1 alpha, were identified as potential contributors to high-temperature tolerance. These DEGs are likely involved in cellular stress response and protein synthesis, facilitating growth under elevated temperatures.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The findings provide new molecular-level insights into the growth mechanisms of <i>N. yezoensis</i> under heat stress. This information can be applied to the development of new cultivars with enhanced growth and heat tolerance, supporting sustainable aquaculture in the face of climate change.</p>\u0000 </section>\u0000 </div>","PeriodicalId":55239,"journal":{"name":"Comparative and Functional Genomics","volume":"2025 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12683680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145714479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to understand the transcriptome and molecular pathways that drive unique functional characteristics of human gingiva.
Materials and Methods
Gene expression data were obtained from the NCBI GEO database, including GSE38617 (connective tissue [CT]) and GSE7224 (gingival epithelium [GE]). Raw data were preprocessed and filtered for healthy samples. Differential gene expression analysis was performed using the limma package in R, with a significance cutoff of log2 fold change ≥ 1 and adjusted p value < 0.05.
Results
The analysis highlighted 11,096 DEGs between the dissected tissues. In CT, 7564 genes were upregulated in extracellular matrix (ECM) organization, collagen synthesis, and immunomodulation. GE had a total of 3532 upregulated genes enriched in epithelial barrier integrity, antimicrobial defense, and keratinocyte differentiation. These patterns were confirmed using key markers (CT: COL1A1 and COL3A1 and GE: DEFB1 and KRT10). In CT, pathway enrichment showed PI3K–Akt signaling and ECM–receptor interaction, whereas GE was characterized by tight junctions and the IL-17 signaling pathway.
Conclusion
The differences in transcriptional landscapes of CT and GE illustrate specialized functions of each tissue type in maintaining periodontal health. Whereas CT focuses on ECM preservation and its immunomodulatory role, GE emphasizes antimicrobial defense and barrier function.
{"title":"Transcriptomic Analysis of Gingival Tissues: Insights Into Gene Expression Profiles","authors":"Saravanan Sampoornam Pape Reddy, Yash Dhiman, Delfin Lovelina Francis, Manish Rathi, Ankit Gupta, Ruchi Harish, Balaji Manohar, Shaili Pradhan","doi":"10.1155/ijog/1427141","DOIUrl":"10.1155/ijog/1427141","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The aim of this study was to understand the transcriptome and molecular pathways that drive unique functional characteristics of human gingiva.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Gene expression data were obtained from the NCBI GEO database, including GSE38617 (connective tissue [CT]) and GSE7224 (gingival epithelium [GE]). Raw data were preprocessed and filtered for healthy samples. Differential gene expression analysis was performed using the limma package in R, with a significance cutoff of log<sub>2</sub> fold change ≥ 1 and adjusted <i>p</i> value < 0.05.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The analysis highlighted 11,096 DEGs between the dissected tissues. In CT, 7564 genes were upregulated in extracellular matrix (ECM) organization, collagen synthesis, and immunomodulation. GE had a total of 3532 upregulated genes enriched in epithelial barrier integrity, antimicrobial defense, and keratinocyte differentiation. These patterns were confirmed using key markers (CT: COL1A1 and COL3A1 and GE: DEFB1 and KRT10). In CT, pathway enrichment showed PI3K–Akt signaling and ECM–receptor interaction, whereas GE was characterized by tight junctions and the IL-17 signaling pathway.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The differences in transcriptional landscapes of CT and GE illustrate specialized functions of each tissue type in maintaining periodontal health. Whereas CT focuses on ECM preservation and its immunomodulatory role, GE emphasizes antimicrobial defense and barrier function.</p>\u0000 </section>\u0000 </div>","PeriodicalId":55239,"journal":{"name":"Comparative and Functional Genomics","volume":"2025 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12683359/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145714423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
<div> <section> <h3> Background</h3> <p>Due to the growth in the global consumption of assisted reproductive technology (ART), it is possible that long-term health impacts on offspring have come into focus. ART has offered a welcome solution to infertility, but the fear has been on its effect on the metabolic health of children born on their behalf. Past studies indicate that ART-conceived individuals can have characteristic metabolic profiles relative to their naturally conceived (NC) peers and are therefore potentially predisposed to changes in lipid and glucose handling. Physiopathological glycolipid metabolism, a hallmark of cardiometabolic health, is believed to be modulated not only by environmental and other external factors but also by intracellular regulation proteins, including sterol regulatory element-binding protein (SREBP) and miR-33, although there is little evidence on the effects of ART on these regulatory pathways in early childhood.</p> </section> <section> <h3> Objective</h3> <p>This paper sought to compare the glycolipid metabolic profile of the kids who are in preschool age and who were conceived through ART and kids who were NC. The second aim was to study the expression of SREBP-1/2 and miR-33 in peripheral blood and the possible nature of the role of these players in regulating early-life metabolism.</p> </section> <section> <h3> Subject and Methodology</h3> <p>A total of 220 children aged between 3 and 6 years were recruited of which complete data has been obtained from 206 children out of 98 that were conceived via in vitro fertilization/intracytoplasmic sperm injection (ICSI) (ART group) and 108 that were conceived naturally (NC group). Anthropometric measures—such as body weight, height, and waist circumference—to determine physical growth and obesity status were taken. Biochemical variables, triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), apolipoprotein A1 (ApoA1), apolipoprotein B (ApoB), fasting serum insulin (FINS), and homeostatic model assessment of insulin resistance (HOMA-IR) were determined. A centrifugal column was used to obtain peripheral blood RNA, and relative gene expression levels of SREBP-1, SREBP-2, miR-33a, and miR-33b were measured by qPCR.</p> </section> <section> <h3> Results</h3> <p>Compared with the IVF group, children in the ICSI group had significantly lower weight, height, and waist circumference (<i>p</i> < 0.05). In contrast, children in the NC group had significantly lower weight, BMI, and waist circumference, with all differences being statistically significant (<i>p</i> < 0.05).
{"title":"Metabolic Status and Expression Level of SREBP mRNA and Mir-33 Among Children Conceived by Assisted Reproductive Technology","authors":"Yifan Cui, Yuqian Wang, Xiaoxia Wang, Yaping Liu, Panpan Zhang, Dan Hu, Xuwu Xiao","doi":"10.1155/ijog/2271298","DOIUrl":"10.1155/ijog/2271298","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Due to the growth in the global consumption of assisted reproductive technology (ART), it is possible that long-term health impacts on offspring have come into focus. ART has offered a welcome solution to infertility, but the fear has been on its effect on the metabolic health of children born on their behalf. Past studies indicate that ART-conceived individuals can have characteristic metabolic profiles relative to their naturally conceived (NC) peers and are therefore potentially predisposed to changes in lipid and glucose handling. Physiopathological glycolipid metabolism, a hallmark of cardiometabolic health, is believed to be modulated not only by environmental and other external factors but also by intracellular regulation proteins, including sterol regulatory element-binding protein (SREBP) and miR-33, although there is little evidence on the effects of ART on these regulatory pathways in early childhood.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This paper sought to compare the glycolipid metabolic profile of the kids who are in preschool age and who were conceived through ART and kids who were NC. The second aim was to study the expression of SREBP-1/2 and miR-33 in peripheral blood and the possible nature of the role of these players in regulating early-life metabolism.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Subject and Methodology</h3>\u0000 \u0000 <p>A total of 220 children aged between 3 and 6 years were recruited of which complete data has been obtained from 206 children out of 98 that were conceived via in vitro fertilization/intracytoplasmic sperm injection (ICSI) (ART group) and 108 that were conceived naturally (NC group). Anthropometric measures—such as body weight, height, and waist circumference—to determine physical growth and obesity status were taken. Biochemical variables, triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), apolipoprotein A1 (ApoA1), apolipoprotein B (ApoB), fasting serum insulin (FINS), and homeostatic model assessment of insulin resistance (HOMA-IR) were determined. A centrifugal column was used to obtain peripheral blood RNA, and relative gene expression levels of SREBP-1, SREBP-2, miR-33a, and miR-33b were measured by qPCR.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Compared with the IVF group, children in the ICSI group had significantly lower weight, height, and waist circumference (<i>p</i> < 0.05). In contrast, children in the NC group had significantly lower weight, BMI, and waist circumference, with all differences being statistically significant (<i>p</i> < 0.05).","PeriodicalId":55239,"journal":{"name":"Comparative and Functional Genomics","volume":"2025 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/ijog/2271298","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145619351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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