Archiv Fur Tierzucht-Archives of Animal Breeding最新文献

This study aimed to investigate the genetic characterization and diversity of Hair goats from 10 regions using 20 microsatellite markers. A total of 522 alleles were observed. The INRA0023 locus exhibited the greatest number of alleles (48), while the DRBP1 locus had the highest effective allele number (16.27), and the BM1818 and DRBP1 loci had the highest polymorphic information content value (0.94). The expected heterozygosity value ranged from 0.85 (ILSTS011) to 0.94 (BM1818, SRCRSP15, and DRBP1). The Hair goat populations in Konya and Hatay displayed the lowest and highest allele numbers, with values of 10.40 and 16.25, respectively. The fixation index ( $F_{\mathrm{IS}}$ ) values are significant in defining population structures and determining the extent of heterozygosity losses. The $F_{\mathrm{IS}}$ values exhibited a range of 0.031 in Muǧla to 0.226 in Burdur. A total of 107 unique alleles were identified in Hair goat populations. However, it is noteworthy that, out of all the alleles, only 25 had a frequency exceeding 5 %. The results indicate that the microsatellite markers utilized demonstrate sufficient levels of polymorphism, making them appropriate for efficiently investigating the genetic variability of Hair goat populations.

The study aimed to examine an effect of the preparation based on nanosilver suspension on mineral carrier on poultry litter microbiological profile. The study was conducted on Ross 308 broiler chickens. Three groups were formed, 84 birds in each. Preparation used in the study was composed of aqueous nanosilver suspension sprayed on mineral sorbent. Birds were maintained on straw-sawdust litter; the groups were differentiated due to preparation application (C - control without preparation, I - preparation applied once at the beginning, II - preparation added each week). Pooled litter samples were collected from the top layer of the litter (six samplings) in order to determine mesophilic bacteria count. Additionally, on the last day of the experiment litter samples were collected from three points (by drinker, feeder, pen corner) to analyze the total number of microorganisms, Salmonella spp., Escherichia coli, Enterococci, and molds. In the case of mesophilic bacteria count, the highest decrease was noted for group II. Total number of microorganisms determined in various points of the pen did not give clear relationship; in some cases even an increase was found. Salmonella spp. decreased as a result of preparation addition; the highest decrease was noted for samples collected by feeders. The results for Escherichia coli are not unequivocal. However, a decrease was found in the case of drinkers and feeders compared to control, especially in group II. An addition of preparation caused a decrease in Enterococci, especially for samples collected by feeders in group II. Similar tendency was found for molds. The study demonstrated that the preparation exhibits bactericidal properties. However, its effect varies depending on microorganism kind and sample collection point.

Kazakh meat-wool sheep are of great interest because of the intrabreed multifetal type's high productivity of 140 %-160 %. Genes encoding growth differentiation factor-9 (GDF9) and bone morphogenetic protein 15 (BMP15) are promising candidates for studying sheep productivity, as they affect fertility in mammals, including sheep. Thus, the purpose of this study was to assess the fertility of the Kazakh meat-wool sheep breed based on GDF9 and BMP15 candidate genes of fecundity for the selection of animals with valuable genotypes. We selected 300 heads of the Kazakh meat-wool sheep breed from two populations for PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) analysis, 15 of which were subsequently used for sequencing of exon regions of the GDF9 and BMP15 genes. The sheep populations were tested for G1 and G8 mutations of the GDF9 gene and B2 and B4 mutations of the BMP15 gene. The PCR-RFLP analysis revealed that 59 (19.7 %) of the 300 Kazakh meat-wool breed sheep were heterozygous carriers of the G1 mutation (genotype AG) of the GDF9 gene, and sequencing analysis supported these results. The comparative phylogenetic analysis showed a clear separation of Kazakh meat-wool sheep wild types and carriers of the G1 mutation. This mutation was reported to have a relationship with the animals' litter size in other sheep breeds. For this reason, similar relationships should be investigated in Kazakh meat-wool sheep. However, G8, B2, and B4 mutations were not detected among the studied animal populations, showing that these mutations are not characteristic of the Kazakh meat-wool sheep breed.

Understanding the molecular mechanism of mammalian reproduction (puberty and prolificacy) will play a part in improving animal reproductive performance. GLUD1 (glutamate dehydrogenase 1) is important for mammalian reproduction, as shown in previous studies; however, its roles in puberty and prolificacy have rarely been reported. In this study, we designed seven pairs of primers (P1 to P7) for cloning and sequencing genomic DNA of Jining Grey goats and Liaoning Cashmere goats. Primer 8 (P8) was designed to detect single nucleotide polymorphism (SNP) of the GLUD1 in both sexually precocious and high-fecundity breeds (Jining Grey, Nanjiang Brown and Matou goats) and sexually late-maturing and low-fecundity breeds (Liaoning Cashmere, Inner Mongolia Cashmere and Taihang goats) by PCR-RFLP (restriction fragment length polymorphism). The real-time quantitative polymerase chain reaction (RT-qPCR) technique was used to detect the expression of GLUD1 in a variety of tissues. The results showed that the A197C mutation was only found in the amplification product of P6. For this SNP locus, only two genotypes (AA and AC) were detected in Nanjiang Brown goats, while three genotypes (AA, AC and CC) were detected in the other five breeds. In Jining Grey goats, the frequency of genotypes AA, AC and CC was 0.69, 0.26 and 0.05, respectively. In Jining Grey goats, AA genotype had 0.54 ($P<0.05$) and 0.3 ($P<0.05$) more kids than the CC and AC genotype, respectively, and no significant difference ($P>0.05$) was found in kidding number between the AC and CC genotype. GLUD1 was expressed in five tissues of different developmental stages. The expression level of GLUD1 in the hypothalamus was higher than that in the other four tissues except during puberty of Liaoning Cashmere goats. In puberty in goats, GLUD1 expression was significantly higher in ovaries than that in the juvenile period ($P<0.01$). RT-qPCR results showed that the expression of GLUD1 in ovaries may relate to the puberty of goats. The present study preliminarily indicated that there might be an association between the 197 locus of GLUD1 and sexual precocity in goats, and allele A of GLUD1 was a potential DNA marker for improving kidding number in Jining Grey goats.

The aim of this study was to determine the effect of two mineral-vitamin premixes on the health status (blood biochemical parameters), milk yield and composition of Holstein-Friesian (HF) cows in Kazakhstan. The study was performed on Holstein-Friesian cows kept on the Bek Plus dairy farm in the village of Korzhynkol, Fyodorovsky District, Kostanay Region. Forty primiparous cows, selected from the herd, were divided by the analogue method into two groups: a control group (C) and an experimental group (E) of 20 animals each. The diets fed to group E cows were supplemented with LI-R 18 PRO and PANTO^® Mineral R-77 Premium mineral-vitamin premixes. The premixes positively affected cow productivity and blood biochemical parameters, whose values were higher in group E than in group C. Higher levels of glucose, albumins and globulins exerted a beneficial influence on the health status of cows. No cases of lameness or metabolic diseases were recorded in group E. The yields of milk, protein and fat were higher in group E than in group C. Dietary supplementation with two premixes had no significant effect on the chemical composition of milk, including the values of the following parameters: somatic cell count (SCC), dry matter (DM), lactose ($L_{\mathrm{se}}$), casein ($C_{\mathrm{in}}$), acidity ($A_{\mathrm{ty}}$), lactic acid (LA), density ($D_{\mathrm{ty}})$, free fatty acids (FFAs), glucose ($G_{\mathrm{se}}$) and urea ($U_{\mathrm{ea}}$).

This study aimed to investigate the impact of single-nucleotide polymorphisms (SNPs) on milk production traits in Kilis dairy goats by analyzing the genotypes of POU1F1, PRLR, $\beta$ -Lg, GH1, and GH2 genes and their association with lactation milk yield (LMY), lactation length (LL) and average daily milk yield (ADMY). Blood samples were collected from 227 goats, and genotyping was performed using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). The results revealed that the frequencies of the genotypes varied among the genes. The polymorphisms were found to be significantly linked with milk production traits. These findings suggest that SNPs of POU1F1/AluI, PRLR/RsaI and $\beta$ -Lg/SacII are significantly associated with LMY and that the POU1F1-TC genotype, PRLR-TT genotype and $\beta$ -Lg-AB genotype are associated with higher LMY and ADMY. Additionally, the POU1F1-TC genotype was found to have a longer LL. However, no significant association was found between the GH1 and GH2 genotypes and LMY, LL and ADMY. Overall, this study provides valuable insights into the genetic factors influencing milk production traits in Kilis dairy goats, which can be utilized for the selection of high-yielding animals in breeding programs.

The litter trait is one of the most important economic traits, and increasing litter size is of great economic value in the pig industry. However, the molecular mechanisms underlying pig litter traits remain elusive. To identify molecular markers and candidate genes for pig litter traits, a genome-wide association study (GWAS) and selection signature analysis were conducted in a Yorkshire pig population. A total of 518 producing sows were genotyped with Illumina Porcine SNP 50 BeadChip, and 1969 farrowing records for the total number born (TNB), the number born alive (NBA), piglets born dead (PBD), and litter weight born alive (LWB) were collected. Then, a GWAS was performed for the four litter traits using a repeatability model. Based on the estimated breeding values (EBVs) of TNB, 15 high- and 15 low-prolificacy individuals were selected from the 518 sows to implement selection signature analysis. Subsequently, the selection signatures affecting the litter traits of sows were detected by using two methods including the fixation index (FST) and $\theta \pi$. Combining the results of the GWAS and selection signature analysis, 20 promising candidate genes (NKAIN2, IGF1R, KISS1R, TYRO3, SPINT1, ADGRF5, APC2, PTBP1, CLCN3, CBR4, HPF1, FAM174A, SCP2, CLIC1, ZFYVE9, SPATA33, KIF5C, EPC2, GABRA2, and GABRA4) were identified. These findings provide novel insights into the genetic basis of pig litter traits and will be helpful for improving the reproductive performances of sows in pig breeding.

The aim of the study was to compare carcass composition and physicochemical and sensory attributes of breast and leg meat from native Mexican guajolote (Meleagris g. gallopavo) as influenced by sex. For this, slaughter weight and carcass characteristics of male ($n=8$) and female ($n=8$) guajolotes raised traditionally under extensive systems with similar housing and feeding conditions were recorded. Also, physical characteristics, proximate composition, the fatty acid profile, and sensory attributes were determined in breast and leg meat using standard procedures. The results showed that males had higher ($P<0.001$) slaughter weight, hot and cold carcass weights, and dressing percentage, as well as carcass part weights, while females had higher ($P<0.001$) abdominal fat weights than males. The lightness ($L^{*}$), yellowness ($b^{*}$), and drip loss values of breast meat, as well as redness ($a^{*}$) and water-holding capacity values of leg meat, were significantly ($P<0.05$) influenced by sex. Male breast meat had higher ($P<0.05$) moisture content, crude protein, erucic acid (C22:1n9), $\sum$ MUFAs (total monounsaturated fatty acids), $\sum$ UFAs (unsaturated fatty acids), $\sum$ DFAs (desirable fatty acids), $\sum$ UFA $/$ $\sum$ SFA (total saturated fatty acid) ratio, $\sum$ PUFA (total polyunsaturated fatty acid) $/$ $\sum$ SFA ratio, and chewiness scores than females. Likewise, leg meat from males showed higher ($P<0.05$) ash content, myristic acid (C14:0), palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1n9c), palmitoleic acid (C16:1n7), $\sum$ SFAs, $\sum$ OFAs (odd fatty acids), thrombogenic index, and atherogenic index, whereas females had high fat content. In conclusion, it would be suggested that, from a nutritional point of view, the meat from male guajolotes was preferable to the meat from females.

The aim of this study was to determine the effect of the pre-mating weight and placental characteristics on birth weight. Data were collected from 62 Karayaka ewes and 70 Karayaka lambs. The placental characteristics considered were placental weight, placental area and the number of cotyledons. The Pearson correlation coefficient was used for statistical comparison and the determination of relationships between variables. In addition, correlation coefficients between live weights and placental characteristics were determined. The average birth weight (BW), pre-mating weight (PMW), placental weight (PW), placental area (PA) and cotyledon number (CN) values were 4.37 $\pm$ 0.70 kg, 50.22 $\pm$ 5.63 kg, 362.51 $\pm$ 118.42 g, 994.18 $\pm$ 312.76 cm${}^2$ and 56.93 $\pm$ 8.06, respectively. BW had positive correlations with PMW (0.147), birth type (BT) (0.643), PW (0.604), PA (0.323) and CN (0.161) ($P$ $<$ 0.05). BW had negative correlations with maternal age (MA) ($-0.119$) ($P$ $<$ 0.05). PMW had positive correlations with maternal age (MA) (0.237) ($P$ $<$ 0.05). PMW had negative correlations with S (sex) ($-0.003$), PW ($-0.049$), PA ($-0.067$) and CN ($-0.080$) ($P$ $>$ 0.05).

The body size and ultrasound carcass traits are related to the growth and muscling of animals. These characters promise future improvement through genetic selection in animal breeding. In breeding programs, knowing the (co)variance components serves to reveal the performance differences among animals and detection of suitable traits for selection. The research was carried out with 313 Anatolian buffalo calves born in 2019 at 36 farm operations. The least-square means for body weight (BW), wither height (WH), rump height (RH), body length (BL), chest width (CW), hip width (HW), chest circumference (CC), cannon-bone circumference (CBC), longissimus muscle area (LMA), longissimus muscle depth (LMD), and subcutaneous fat thickness (SFT) in yearling calves were 175.41 $\pm$ 2.06 kg, 108.35 $\pm$ 0.34, 111.85 $\pm$ 0.37, 103.74 $\pm$ 0.41, 33.93 $\pm$ 0.23, 30.56 $\pm$  0.23, 135.18 $\pm$ 0.60, 15.69 $\pm$ 0.08 cm, 19.36 $\pm$ 0.45 cm${}^2$, 3.086 $\pm$ 0.028, and 0.655 $\pm$ 0.006 cm, respectively. The direct heritabilities for BW, WH, RH, BL, CW, HW, CC, CBC, LMA, LMD, and SFT were 0.334 $\pm$ 0.032, 0.483 $\pm$ 0.044, 0.473 $\pm$ 0.043, 0.441 $\pm$ 0.041, 0.364 $\pm$ 0.034, 0.432 $\pm$ 0.040, 0.435 $\pm$ 0.040, 0.226 $\pm$ 0.021, 0.0001 $\pm$ 0.000, 0.300 $\pm$ 0.026, and 0.539 $\pm$ 0.046, respectively. The genetic and phenotypic correlations predicted in this study ranged from 0.02 to 0.90. All the genetic and phenotypic correlations among body size and ultrasound carcass traits were significant ($P<0.01$), except for the genetic correlation between CW and HW. Some polymorphisms in PLAG1, NCAPG, LCORL, and HMGA2 genes were analyzed. Two single-nucleotide polymorphisms (SNPs) for PLAG1 and NCAPG genes were found to be monomorphic in this buffalo population. Meanwhile, the effects of two SNPs in the LCORL and HMGA2 genes were not significant but showed some tendencies in the aspects of least-square means. The results of the study indicated that the Anatolian buffaloes have the potential to improve in growth and muscling characteristics.