Pub Date : 2023-01-01DOI: 10.12677/hjbm.2023.132031
振伟 秦
Objective: To explore whether the crude extract of clove alcohol at 39 ˚C affects the proliferation, migration, invasion and apoptosis of pancreatic cancer cells. Methods: 1) CCK8 assay to detect the effect of crude extract of clove alcohol on the proliferation activity of pancreatic cancer cells at 39 ˚C ; 2) plate clone formation and soft agar cloning assay to detect the effect of crude extract of clove alcohol on the growth and proliferation of pancreatic cancer cells at 39 ˚C ; 3) scratch assay and Transwell assay to detect the effect of crude extract of clove alcohol on pancreatic cancer cells at 39 ˚C ; 4) Hoechst 33258 staining and flow cytometry to detect the effect of clove alcohol extract on cell morphological changes and apoptosis at 39 ˚C . Results: The results of CCK8 showed that the crude extract of clove alcohol could inhibit the growth and proliferation of PANC-1 cells, and the inhibitory effect was concentration-dependent, and the effect was more obvious in combination with 39 ˚C . The results of the scratch test showed that the crude extract of clove alcohol could inhibit the migration of PANC-1 cells, and the effect was more obvious in combination with 39°C, but there was no significant change between 37 ˚C and 39 ˚C control . The results of Hoechst33258 experiment showed that the crude extract of clove alcohol could cause the nucleus of PANC-1 cells to appear bright blue, apoptotic vesicles and nuclear fragmentation. The flow cytometry also verified that the crude extract of clove alcohol could induce apoptosis in PANC-1 cells with an increase in the number of apoptotic cells at 39 ˚C . Conclusion: The crude extract of clove alcohol can inhibit cell growth and proliferation, inhibit cell migration and invasion and induce apoptosis, and the effect is more obvious in combination with 39 ˚C , thus exerting anti-tumor effects from different mechanisms
{"title":"Effect of Crude Extract of Clove Alcohol on the Biological Behaviour of PANC-1 Cells at 39?C","authors":"振伟 秦","doi":"10.12677/hjbm.2023.132031","DOIUrl":"https://doi.org/10.12677/hjbm.2023.132031","url":null,"abstract":"Objective: To explore whether the crude extract of clove alcohol at 39 ˚C affects the proliferation, migration, invasion and apoptosis of pancreatic cancer cells. Methods: 1) CCK8 assay to detect the effect of crude extract of clove alcohol on the proliferation activity of pancreatic cancer cells at 39 ˚C ; 2) plate clone formation and soft agar cloning assay to detect the effect of crude extract of clove alcohol on the growth and proliferation of pancreatic cancer cells at 39 ˚C ; 3) scratch assay and Transwell assay to detect the effect of crude extract of clove alcohol on pancreatic cancer cells at 39 ˚C ; 4) Hoechst 33258 staining and flow cytometry to detect the effect of clove alcohol extract on cell morphological changes and apoptosis at 39 ˚C . Results: The results of CCK8 showed that the crude extract of clove alcohol could inhibit the growth and proliferation of PANC-1 cells, and the inhibitory effect was concentration-dependent, and the effect was more obvious in combination with 39 ˚C . The results of the scratch test showed that the crude extract of clove alcohol could inhibit the migration of PANC-1 cells, and the effect was more obvious in combination with 39°C, but there was no significant change between 37 ˚C and 39 ˚C control . The results of Hoechst33258 experiment showed that the crude extract of clove alcohol could cause the nucleus of PANC-1 cells to appear bright blue, apoptotic vesicles and nuclear fragmentation. The flow cytometry also verified that the crude extract of clove alcohol could induce apoptosis in PANC-1 cells with an increase in the number of apoptotic cells at 39 ˚C . Conclusion: The crude extract of clove alcohol can inhibit cell growth and proliferation, inhibit cell migration and invasion and induce apoptosis, and the effect is more obvious in combination with 39 ˚C , thus exerting anti-tumor effects from different mechanisms","PeriodicalId":65695,"journal":{"name":"生物医学","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66256393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.12677/hjbm.2023.132028
鹏 王
{"title":"The Mechanism and Research Progress of Platelet Affecting Liver Regeneration","authors":"鹏 王","doi":"10.12677/hjbm.2023.132028","DOIUrl":"https://doi.org/10.12677/hjbm.2023.132028","url":null,"abstract":"","PeriodicalId":65695,"journal":{"name":"生物医学","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66256257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.12677/hjbm.2023.132012
洁莹 丁
{"title":"Mechanism of Action of Phytonutrients on Alzheimer’s Disease","authors":"洁莹 丁","doi":"10.12677/hjbm.2023.132012","DOIUrl":"https://doi.org/10.12677/hjbm.2023.132012","url":null,"abstract":"","PeriodicalId":65695,"journal":{"name":"生物医学","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66256308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.12677/hjbm.2023.131006
鹤润 王
{"title":"Preparation of AgNPs@ZIF-8@HA and Its Antibacterial Activities against Two Drug-Resistant Bacteria in Vitro","authors":"鹤润 王","doi":"10.12677/hjbm.2023.131006","DOIUrl":"https://doi.org/10.12677/hjbm.2023.131006","url":null,"abstract":"","PeriodicalId":65695,"journal":{"name":"生物医学","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66256018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.12677/hjbm.2023.132032
文赫 张
Transient receptor potential (TRP) channels belong to a large and diverse family of integrins, which appear as cellular sensors for thermal, chemical and mechanical stimulation
{"title":"TRP Channels and Inherited Diseases","authors":"文赫 张","doi":"10.12677/hjbm.2023.132032","DOIUrl":"https://doi.org/10.12677/hjbm.2023.132032","url":null,"abstract":"Transient receptor potential (TRP) channels belong to a large and diverse family of integrins, which appear as cellular sensors for thermal, chemical and mechanical stimulation","PeriodicalId":65695,"journal":{"name":"生物医学","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66256404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.12677/hjbm.2023.132017
{"title":"Study on the Mechanism and Influence of Cognitive Training on Alzheimer’s Disease","authors":"","doi":"10.12677/hjbm.2023.132017","DOIUrl":"https://doi.org/10.12677/hjbm.2023.132017","url":null,"abstract":"","PeriodicalId":65695,"journal":{"name":"生物医学","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66256450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.12677/hjbm.2023.132033
义 蒋
Objective: The influence of frozen time on the test results of ALT quality control products, was ana-*
{"title":"The Effect of Freezing Time on the Detection of ALT Dry Powder Quality Control Products","authors":"义 蒋","doi":"10.12677/hjbm.2023.132033","DOIUrl":"https://doi.org/10.12677/hjbm.2023.132033","url":null,"abstract":"Objective: The influence of frozen time on the test results of ALT quality control products, was ana-*","PeriodicalId":65695,"journal":{"name":"生物医学","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66256464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.12677/hjbm.2023.132026
金艳 屈
{"title":"Effect of Haben Aqua Atomization Skincare Pro in Combination with Haben Sodium Hyaluronate Moisturizing and Soothing Essence on Moisture Content of Cuticle","authors":"金艳 屈","doi":"10.12677/hjbm.2023.132026","DOIUrl":"https://doi.org/10.12677/hjbm.2023.132026","url":null,"abstract":"","PeriodicalId":65695,"journal":{"name":"生物医学","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66256692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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