世界胃肠病理生理学杂志(电子版)(英文版)最新文献_第5页

Novel, non-colonizing, single-strain live biotherapeutic product ADS024 protects against Clostridioides difficile infection challenge in vivo. 新型、非定植性、单一菌株的活生物治疗产品ADS024可在体内抵御艰难梭菌感染挑战。

世界胃肠病理生理学杂志(电子版)(英文版)

Pub Date : 2023-08-24 DOI: 10.4291/wjgp.v14.i4.71

Christopher K Murphy, Michelle M O'Donnell, James W Hegarty, Sarah Schulz, Colin Hill, R Paul Ross, Mary C Rea, Ronald Farquhar, Laurent Chesnel

Background: The Centers for Disease Control and Prevention estimate that Clostridioides difficile (C. difficile) causes half a million infections (CDI) annually and is a major cause of total infectious disease death in the United States, causing inflammation of the colon and potentially deadly diarrhea. We recently reported the isolation of ADS024, a Bacillus velezensis (B. velezensis) strain, which demonstrated direct in vitro bactericidal activity against C. difficile, with minimal collateral impact on other members of the gut microbiota. In this study, we hypothesized that in vitro activities of ADS024 will translate in vivo to protect against CDI challenge in mouse models.

Aim: To investigate the in vivo efficacy of B. velezensis ADS024 in protecting against CDI challenge in mouse models.

Methods: To mimic disruption of the gut microbiota, the mice were exposed to vancomycin prior to dosing with ADS024. For the mouse single-dose study, the recovery of ADS024 was assessed via microbiological analysis of intestinal and fecal samples at 4 h, 8 h, and 24 h after a single oral dose of 5 × 10⁸ colony-forming units (CFU)/mouse of freshly grown ADS024. The single-dose study in miniature swine included groups that had been pre-dosed with vancomycin and that had been exposed to a dose range of ADS024, and a group that was not pre-dosed with vancomycin and received a single dose of ADS024. The ADS024 colonies [assessed by quantitative polymerase chain reaction (qPCR) using ADS024-specific primers] were counted on agar plates. For the 28-d miniature swine study, qPCR was used to measure ADS024 levels from fecal samples after oral administration of ADS024 capsules containing 5 × 10⁹ CFU for 28 consecutive days, followed by MiSeq compositional sequencing and bioinformatic analyses to measure the impact of ADS024 on microbiota. Two studies were performed to determine the efficacy of ADS024 in a mouse model of CDI: Study 1 to determine the effects of fresh ADS024 culture and ADS024 spore preparations on the clinical manifestations of CDI in mice, and Study 2 to compare the efficacy of single daily doses vs dosing 3 times per day with fresh ADS024. C. difficile challenge was performed 24 h after the start of ADS024 exposure. To model the human distal colon, an anerobic fecal fermentation system was used. MiSeq compositional sequencing and bioinformatic analyses were performed to measure microbiota diversity changes following ADS024 treatment. To assess the potential of ADS024 to be a source of antibiotic resistance, its susceptibility to 18 different antibiotics was tested.

Results: In a mouse model of CDI challenge, single daily doses of ADS024 were as efficacious as multiple daily doses in protecting against subsequent challenge by C. difficile

背景：美国疾病控制与预防中心估计，艰难梭菌（C.difficile）每年导致50万例感染（CDI），是美国全部传染病死亡的主要原因，导致结肠炎症和潜在的致命腹泻。我们最近报道了ADS024的分离，这是一种维莱岑芽孢杆菌（B.velezensis）菌株，它对艰难梭菌具有直接的体外杀菌活性，对肠道微生物群的其他成员的副作用最小。在这项研究中，我们假设ADS024的体外活性将在体内转化，以保护小鼠模型免受CDI攻击。目的：研究B.velezensis ADS024在小鼠模型中对CDI攻击的体内保护作用。方法：为了模拟肠道微生物群的破坏，小鼠在给药ADS024之前暴露于万古霉素。对于小鼠单剂量研究，在单次口服5×108菌落形成单位（CFU）/小鼠的新鲜生长的ADS024后4小时、8小时和24小时，通过肠道和粪便样本的微生物分析来评估ADS024的回收率。在小型猪中进行的单剂量研究包括预先给药万古霉素并暴露于ADS024剂量范围的组，以及未预先给药阿霉素并接受单剂量ADS024的组。在琼脂平板上计数ADS024菌落[通过使用ADS024特异性引物的定量聚合酶链式反应（qPCR）评估]。在28天的小型猪研究中，连续28天口服含有5×109CFU的ADS024胶囊后，使用qPCR测量粪便样本中的ADS2024水平，然后进行MiSeq成分测序和生物信息学分析，以测量ADS024对微生物群的影响。进行了两项研究来确定ADS024在CDI小鼠模型中的疗效：研究1确定新鲜ADS024培养物和ADS024孢子制剂对小鼠CDI临床表现的影响，研究2比较每日单次给药与每日3次给药的疗效。艰难梭菌激发在ADS024暴露开始后24小时进行。为了模拟人类远端结肠，使用了厌氧粪便发酵系统。进行MiSeq成分测序和生物信息学分析，以测量ADS024处理后微生物群多样性的变化。为了评估ADS024作为抗生素耐药性来源的潜力，测试了其对18种不同抗生素的易感性。结果：在CDI攻击的小鼠模型中，单次每日剂量的ADS024在预防艰难梭菌病原体诱导的疾病的后续攻击方面与多次每日剂量的一样有效。基于在小鼠中单次给药后24小时或在小型猪中单次剂量后72小时未恢复ADS024菌落的观察结果，ADS024未显示定植的证据。在对小型猪进行的28天重复剂量研究中，使用平板和qPCR方法在粪便样本中未检测到ADS024。在人类远端结肠模型中进行的系统发育分析表明，ADS024对健康的人类结肠微生物群具有选择性影响，类似于在小型猪中进行的体内研究。安全性评估表明，ADS024对所有测试的抗生素都敏感，而计算机测试显示，脱靶活性或毒力和抗生素耐药性机制的可能性很低。结论：我们的研究结果证明了ADS024在小鼠模型中对CDI攻击的体内保护作用，支持使用ADS024预防标准抗生素治疗后复发的CDI。

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引用次数: 0

Risk assessment of hepatitis E transmission through tissue allografts 同种异体组织移植传播戊型肝炎的风险评估

世界胃肠病理生理学杂志(电子版)(英文版)

Pub Date : 2022-03-22 DOI: 10.4291/wjgp.v13.i2.50

R. Villalba, V. Mirabet

Hepatitis E virus (HEV) is a small non-enveloped single stranded RNA virus whose genotypes 3 and 4 have been associated with zoonotic transmission in industrialized countries. HEV infection is considered the main cause of acute hepatitis worldwide. In some cases, transfusion of blood components or organ transplantation have been reported as the source of infection. We have conducted a literature review on the risk of transmission through cell and tissue allografts. Although no case was found, measures to control this risk should be taken when donor profile (based upon geographical and behavioural data) recommended it. Issues to be considered in donor screening and tissue processing to assess and to reduce the risk of HEV transmission are approached.

戊型肝炎病毒(HEV)是一种小型无包膜单链RNA病毒，其基因型3和4与工业化国家的人畜共患传播有关。在世界范围内，HEV感染被认为是急性肝炎的主要原因。在某些情况下，输血或器官移植被报道为感染源。我们对通过细胞和组织同种异体移植传播的风险进行了文献回顾。虽然没有发现病例，但当捐助者情况(根据地理和行为数据)建议时，应采取措施控制这种风险。探讨了在供体筛选和组织处理中需要考虑的问题，以评估和降低戊型肝炎传播的风险。

引用次数: 1

Application of electron microscopy in gastroenterology 电子显微镜在胃肠病学中的应用

世界胃肠病理生理学杂志(电子版)(英文版)

Pub Date : 2022-03-22 DOI: 10.4291/wjgp.v13.i2.41

M. Iwamuro, Haruo Urata, Takehiro Tanaka, Hiroyuki Okada

Electron microscopy has long been used in research in the fields of life sciences and materials sciences. Transmission and scanning electron microscopy and energy-dispersive X-ray spectroscopy (EDX) analyses have also been performed in the field of gastroenterology. Electron microscopy and EDX enable (1) Observation of ultrastructural differences in esophageal epithelial cells in patients with gastroesophageal reflux and eosinophilic esophagitis; (2) Detection of lanthanum deposition in the stomach and duodenum; (3) Ultrastructural and elemental analyses of enteroliths and bezoars; (4) Detection and characterization of microorganisms in the gastrointestinal tract; (5) Diagnosis of gastrointestinal tumors with neuroendocrine differentiation; and (6) Analysis of gold nanoparticles potentially used in endoscopic photodynamic therapy. This review aims to foster a better understanding of electron microscopy applications by reviewing relevant clinical studies, basic research findings, and the state of current research carried out in gastroenterology science.

电子显微镜在生命科学和材料科学领域的研究中应用已久。透射和扫描电子显微镜和能量色散x射线光谱(EDX)分析也在胃肠病学领域进行。(1)胃食管反流与嗜酸性粒细胞性食管炎患者食管上皮细胞超微结构差异的观察;(2)胃、十二指肠镧沉积检测;(3)肠石和牛黄的超微结构和元素分析;(4)胃肠道微生物的检测与鉴定;(5)胃肠道肿瘤神经内分泌分化诊断;(6)金纳米颗粒在内窥镜光动力治疗中的潜在应用分析。本文旨在通过对胃肠病学相关临床研究、基础研究成果和研究现状的综述，加深对电子显微镜应用的了解。

引用次数: 0

Comparison of cytokine and phosphoprotein profiles in idiopathic and Crohn’s disease-related perianal fistula 特发性和克罗恩病相关肛周瘘的细胞因子和磷蛋白谱比较

世界胃肠病理生理学杂志(电子版)(英文版)

Pub Date : 2019-11-13 DOI: 10.4291/wjgp.v10.i4.42

J. Haddow, O. Musbahi, T. Macdonald, C. Knowles

BACKGROUND Perianal fistulae are either primary (idiopathic) or secondary [commonly associated with Crohn’s disease, (CD)]. It is assumed, although not proven, that the pathophysiology differs. AIM To systematically compare the clinical phenotypes, cytokine and phosphoprotein profiles of idiopathic and CD-related perianal fistulae. METHODS Sixty-one patients undergoing surgery for perianal fistula were prospectively recruited (48 idiopathic, 13 CD) into a cohort study. Clinical data, including the Perineal Disease Activity Index (PDAI) and EQ-5D-5L were collected. Biopsies of the fistula tract, granulation tissue, internal opening mucosa and rectal mucosa were obtained at surgery. Concentrations of 30 cytokines and 39 phosphoproteins were measured in each biopsy using a magnetic bead multiplexing instrument and a chemiluminescent antibody array respectively. Over 12000 clinical and 23500 laboratory measurements were made. RESULTS The PDAI was significantly higher (indicating more active disease) in the CD group with a mean difference of 2.40 (95%CI: 0.52-4.28, P = 0.01). Complex pathoanatomy was more prevalent in the CD group, namely more multiple fistulae, supralevator extensions, collections and rectal thickening. The IL-12p70 concentration at the internal opening specimen site was significantly higher (median difference 19.7 pg/mL, 99%CI: 0.2-40.4, P = 0.008) and the IL-1RA/IL-1β ratio was significantly lower in the CD group at the internal opening specimen site (median difference 15.0, 99%CI = 0.4-50.5, P = 0.008). However in the remaining 27 cytokines and all 39 of the phosphoproteins across the four biopsy sites, no significant differences were found between the groups. CONCLUSION CD-related perianal fistulae are more clinically severe and anatomically complex than idiopathic perianal fistulae. However, overall there are no major differences in cytokine and phosphoprotein profiles.

背景肛瘘是原发性（特发性）或继发性[通常与克罗恩病（CD）有关]。虽然没有得到证实，但病理生理学是不同的。目的系统比较特发性和CD相关性肛周瘘的临床表型、细胞因子和磷蛋白谱。方法前瞻性招募61例接受肛周瘘手术的患者（48例特发性，13例CD）进行队列研究。收集临床数据，包括腓疾病活动指数（PDAI）和EQ-5D-5L。术中对瘘管、肉芽组织、内口黏膜和直肠黏膜进行了活检。分别使用磁珠多路复用仪器和化学发光抗体阵列在每次活检中测量30种细胞因子和39种磷蛋白的浓度。进行了超过12000次临床和23500次实验室测量。结果CD组的PDAI明显高于对照组（表明疾病更活跃），平均差异为2.40（95%CI:0.52-42.28，P=0.01）。CD组更常见复杂的病理解剖，即多发瘘、提上肌扩张、集合和直肠增厚。CD组在内部开放标本部位的IL-12p70浓度显著较高（中位差异19.7 pg/mL，99%CI:0.2-40.4，P=0.008），IL-1RA/IL-1β比率显著较低（中位差值15.0，99%CI=0.4-50.5，P=0.008活检部位，两组之间没有发现显著差异。结论CD相关性肛周瘘比特发性肛周瘘在临床上更严重，在解剖学上更复杂。然而，总体而言，细胞因子和磷蛋白谱没有重大差异。

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引用次数: 8

Neutropenic enterocolitis: A clinico-pathological review 中性粒细胞减少性小肠结肠炎:临床病理回顾

世界胃肠病理生理学杂志(电子版)(英文版)

Pub Date : 2019-10-15 DOI: 10.4291/wjgp.v10.i3.36

R. Xia, Xuchen Zhang

Neutropenic enterocolitis (NE) is a predominantly cecum-based disease with high mortality seen in patients post chemotherapy. The pathogenesis of NE is poorly understood and probably multifactorial involving mucosal injury, neutropenia, and impaired host defense to intestinal organisms. The clinical presentation is characterized as ileocolonic inflammation and bowel wall thickening in patients with neutropenia, fever, and abdominal pain. The pathological features of NE include patchy necrosis, hemorrhage, ulcer, edema, perforation, infiltrating organisms, and characteristically, depletion of inflammatory cells (neutrophils). NE should always be considered as a possible diagnosis in immunosuppressed patients, especially those receiving chemotherapy. High clinical and histological diagnostic discordance rate exists. High index of clinical suspicion and prompt appropriate personalized management are essential to achieve a lower mortality rate.

中性粒细胞减少性小肠结肠炎(NE)是一种主要以盲肠为基础的疾病，化疗后患者死亡率高。NE的发病机制尚不清楚，可能是多因素的，包括粘膜损伤、中性粒细胞减少和宿主对肠道生物的防御受损。临床表现为回结肠炎症和肠壁增厚，伴有中性粒细胞减少、发热和腹痛。NE的病理特征包括斑片状坏死、出血、溃疡、水肿、穿孔、浸润性生物，以及典型的炎症细胞(中性粒细胞)耗竭。对于免疫抑制的患者，尤其是接受化疗的患者，应始终将NE视为可能的诊断。存在较高的临床和组织学诊断不符合率。高临床怀疑指数和及时适当的个性化治疗是实现低死亡率的必要条件。

引用次数: 21

Immune checkpoint inhibitor-induced diarrhea/colitis: Endoscopic and pathologic findings. 免疫检查点抑制剂诱导的腹泻/结肠炎：内镜和病理结果

世界胃肠病理生理学杂志(电子版)(英文版)

Pub Date : 2019-09-10 DOI: 10.4291/wjgp.v10.i2.17

Tsutomu Nishida, Hideki Iijima, Shiro Adachi

The indications of immune checkpoint inhibitors (ICPIs) for cancer treatment have rapidly expanded, and their use is increasing in clinical settings worldwide. Despite the considerable clinical benefits of ICPIs, frequent immune-related adverse events (irAEs) have become nonnegligible concerns. Among irAEs, ICPI-induced colitis/diarrhea is frequent and recognized not only by oncologists but also by gastroenterologists or endoscopists. The endoscopic findings show similarity to those of inflammatory bowel disease to a certain extent, particularly ulcerative colitis, but do not seem to be identical. The pathological findings of ICPI-induced colitis may vary among drug classes. They show acute or chronic inflammation, but it may depend on the time of colitis suggested by colonoscopy, including biopsy or treatment intervention. In the case of chronic inflammation determined by biopsy, the endoscopy findings may overlap with those of inflammatory bowel disease. Here, we provide a comprehensive review of ICPI-induced colitis based on clinical, endoscopic and pathologic findings.

免疫检查点抑制剂（ICPI）用于癌症治疗的适应症迅速扩大，其在全球临床环境中的应用也在增加。尽管ICPI具有相当大的临床益处，但频繁的免疫相关不良事件（irAE）已成为不可忽视的问题。在irAE中，ICPI诱导的结肠炎/腹泻是常见的，不仅肿瘤学家认识到，胃肠病学家或内镜学家也认识到。内镜检查结果在一定程度上与炎症性肠病，特别是溃疡性结肠炎的检查结果相似，但似乎并不相同。ICPI诱导的结肠炎的病理学表现可能因药物类别而异。它们表现为急性或慢性炎症，但可能取决于结肠镜检查建议的结肠炎时间，包括活检或治疗干预。在通过活检确定的慢性炎症的情况下，内窥镜检查结果可能与炎症性肠病的结果重叠。在此，我们根据临床、内镜和病理结果对ICPI诱导的结肠炎进行了全面的综述。

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引用次数: 0

Successful management of adhesion related small bowel ischemia without intestinal resection: A case report and review of literature 无肠切除术成功治疗粘连性小肠缺血1例报告及文献复习

世界胃肠病理生理学杂志(电子版)(英文版)

Pub Date : 2019-09-10 DOI: 10.4291/wjgp.v10.i2.29

P. Vassiliu, Vasiliki Ntella, George Theodoroleas, Zisis Mantanis, Ioanna Pentara, E. Papoutsi, A. Mastoraki, N. Arkadopoulos

BACKGROUND Intraabdominal adhesions develop spontaneously or after an inflammatory process or surgical procedure in the abdomen. They are the most common cause of small bowel obstruction (SBO). SBO occasionally leads to intestinal ischemia (InIs) which can be a life-threatening condition that requires management as soon as possible. We herein report a case of SBO with InIs presented in our institution and treated without intestinal resection. CASE SUMMARY A 34-year-old man presented at the emergency department after a 12-h-onset diffuse abdominal pain, bloating and nausea. He had a history of traumatic right hepatectomy 11 years ago as well as adhesiolysis and resection of a long part of small bowel 2 years ago. An abdominal computed tomography (CT) showed dilated loops that led to the diagnosis of SBO. Due to deteriorating lactic acidosis, the patient was operated. Torsion of the small bowel around an adhesion led to 2.30 m of ischemic ileum. After the application of N/S 40 °C for 20 min, the intestine showed signs of improvement and it was decided to avoid resection and instead temporary close the abdomen with vacuum-pack technique. At the second-look laparotomy 48 h later, the intestine appeared normal. The patient was discharged on the 8th post-op day in excellent condition. CONCLUSION In case of SBO caused by adhesions, extreme caution is needed if InIs is present, as the clinical signs are mild and you should rely for diagnosis in CT findings and lactate levels. Conservative surgical approach could reverse the effects of InIs, if performed quickly, so that intestinal resection is avoided and should be used even when minimum signs of viability are present.

背景腹腔粘连是自发发生的，或者是在腹部炎症过程或外科手术后发生的。它们是小肠梗阻（SBO）最常见的原因。SBO偶尔会导致肠缺血（InIs），这可能是一种危及生命的情况，需要尽快治疗。我们在此报告了一例在我们的机构中出现的伴有InIs的SBO，并在没有进行肠道切除的情况下进行了治疗。病例摘要：一名34岁男子在发病12小时后出现弥漫性腹痛、腹胀和恶心，随后到急诊科就诊。他11年前有外伤性右肝切除术的病史，2年前有粘连松解术和小肠长部切除术。腹部计算机断层扫描（CT）显示扩张环，从而诊断为SBO。由于乳酸酸中毒恶化，病人接受了手术。粘连周围的小肠扭转导致2.30米的缺血性回肠。在应用N/S 40°C 20分钟后，肠道出现好转迹象，决定避免切除，而是用真空包技术暂时闭合腹部。48小时后第二次剖腹探查时，肠道显示正常。患者于术后第8天出院，情况良好。结论在由粘连引起的SBO的情况下，如果存在InIs，则需要格外小心，因为临床症状较轻，应根据CT检查结果和乳酸水平进行诊断。如果快速进行，保守的手术方法可以逆转InIs的影响，从而避免肠道切除，即使在存在最低生存迹象的情况下也应该使用。

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引用次数: 3

Overview of studies of the vitamin D/vitamin D receptor system in the development of non-alcoholic fatty liver disease 维生素D/维生素D受体系统在非酒精性脂肪肝发病中的研究综述

世界胃肠病理生理学杂志(电子版)(英文版)

Pub Date : 2019-09-10 DOI: 10.4291/wjgp.v10.i2.11

F. A. Cimini, I. Barchetta, S. Carotti, S. Morini, M. Cavallo

Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease in the world. NAFLD is known to be associated with obesity, type 2 diabetes, metabolic syndrome and increased cardiovascular events: for these reasons, it is becoming a global public health problem and represents an important challenge in terms of prevention and treatment. The mechanisms behind the pathogenesis of NAFLD are multiple and have not yet been completely unraveled; consequently, at moment there are not effective treatments. In the past few years a large body of evidence has been assembled that attributes an important role in hepatic aberrant fat accumulation, inflammation and fibrosis, to the vitamin D/vitamin D receptor (VD/VDR) axis, showing a strong association between hypovitaminosis D and the diagnosis of NAFLD. However, the data currently available, including clinical trials with VD supplementation, still provides a contrasting picture. The purpose of this editorial is to provide an overview of recent advances in the pathogenesis of NAFLD in relation to VD/VDR. Based on recent data from literature, we focused in particular on the hypothesis that VDR itself, independently from its traditional ligand VD, may have a crucial function in promoting hepatic fat accumulation. This might also offer new possibilities for future innovative therapeutic approaches in the management of NAFLD.

非酒精性脂肪性肝病(NAFLD)是世界上最常见的慢性肝病。众所周知，NAFLD与肥胖、2型糖尿病、代谢综合征和心血管事件增加有关:由于这些原因，它正在成为一个全球性的公共卫生问题，并在预防和治疗方面提出了重要挑战。NAFLD发病机理背后的机制是多种的，尚未完全解开;因此，目前还没有有效的治疗方法。在过去的几年中，大量的证据表明，维生素D/维生素D受体(VD/VDR)轴在肝脏异常脂肪积累、炎症和纤维化中起着重要作用，表明维生素D缺乏症与NAFLD的诊断之间存在很强的关联。然而，目前可用的数据，包括补充VD的临床试验，仍然提供了一个相反的画面。这篇社论的目的是概述与VD/VDR相关的NAFLD发病机制的最新进展。基于最近的文献数据，我们特别关注了这样一个假设，即VDR本身，独立于其传统的配体VD，可能在促进肝脏脂肪积累方面具有关键功能。这也可能为未来NAFLD管理的创新治疗方法提供新的可能性。

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引用次数: 12

Microscopic colitis in patients with mild duodenal damage: A new clinical and pathological entity (“lymphocytic enterocolitis”)? 十二指肠轻度损伤患者的镜下结肠炎:一种新的临床和病理实体(“淋巴细胞性小肠结肠炎”)?

世界胃肠病理生理学杂志(电子版)(英文版)

Pub Date : 2016-11-15 DOI: 10.4291/wjgp.v7.i4.307

G. Bonagura, D. Ribaldone, S. Fagoonee, N. Sapone, G. P. Caviglia, G. Saracco, M. Astegiano, R. Pellicano

AIM To evaluate the potential association between mild duodenal damage and microscopic colitis (MC). METHODS We retrospectively included 105 consecutive patients with type I Marsh-Oberhuber duodenal damage and negativity for immunoglobulin A anti-endomysium and anti-tissue transglutaminase. The following parameters were analyzed: Sex, age at execution of esophagogastroduodenoscopy, duodenal damage, and number of intraepithelial lymphocytes at biopsies, prevalence of Helicobacter pylori infection, age at execution of colonoscopy, macroscopic and microscopic features of colonoscopy, family history of gastrointestinal and autoimmune diseases, smoking habits, biochemical parameters of inflammation and autoimmunity, use of proton pump inhibitors or nonsteroidal anti-inflammatory drugs, adverse reactions to drugs or foods, pathologies known to be associated with celiac disease or MC, living on a gluten-free diet or on a gluten-low diet for at least 1 mo. RESULTS Colonoscopy was performed in 59 patients, but only in 48 of them biopsies were taken in the entire colon. Considering the latter cohort, the diagnosis of MC was met in 25 (52.1%) patients while in 18 patients other pathologic findings were reported: 13 (27%) cases of nonspecific inflammatory bowel disease, 2 (4.2%) cases of Crohn’s disease, 2 (4.2%) cases of eosinophilic gastroenteritis, and 1 (2.1%) case of autoimmune enteritis. Five (10.4%) patients had a normal colonoscopic result. Matching the groups by age, and considering only patients who underwent colonoscopy (42.7 ± 15.5 years) vs those who did not undergo colonoscopy (36.9 ± 10.6 years), a statistical difference was found (P = 0.039). Focusing on symptoms, diarrhea was statistically more prevalent in MC group than in patients who did not undergo colonoscopy (P = 0.03). CONCLUSION Mild duodenal damage is associated with MC in more than half of the cases. This association supports the hypothesis of a link between these two entities.

目的探讨十二指肠轻度损伤与显微镜下结肠炎(MC)的潜在关系。方法我们回顾性地纳入105例连续的I型Marsh-Oberhuber十二指肠损伤且免疫球蛋白A抗肌内膜和抗组织转谷氨酰胺酶阴性的患者。分析了以下参数:性别、食管胃十二指肠镜检查年龄、十二指肠损伤、活检上皮内淋巴细胞数量、幽门螺杆菌感染患病率、结肠镜检查年龄、宏观和微观特征、胃肠道和自身免疫性疾病家族史、吸烟习惯、炎症和自身免疫生化指标、质子泵抑制剂或非甾体抗炎药的使用、药物或食物的不良反应、已知与乳糜泻或乳糜泻相关的病理，生活在无麸质饮食或低麸质饮食中至少1个月。结果59例患者进行了结肠镜检查，但其中只有48例患者在整个结肠进行了活检。在后一组中，25例(52.1%)患者诊断为MC，而18例患者报告了其他病理结果:13例(27%)为非特异性炎症性肠病，2例(4.2%)为克罗恩病，2例(4.2%)为嗜酸性胃肠炎，1例(2.1%)为自身免疫性肠炎。5例(10.4%)患者结肠镜检查结果正常。按年龄进行分组匹配，仅考虑结肠镜检查组(42.7±15.5岁)与未结肠镜检查组(36.9±10.6岁)，差异有统计学意义(P = 0.039)。关注症状，MC组腹泻发生率高于未行结肠镜检查的患者(P = 0.03)。结论半数以上的病例伴有轻度十二指肠损伤。这种联系支持了这两个实体之间存在联系的假设。

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引用次数: 3

Anti-Helicobacter pylori effect of CaG-NANA, a new sialic acid derivative 新型唾液酸衍生物CaG-NANA的抗幽门螺杆菌作用

世界胃肠病理生理学杂志(电子版)(英文版)

Pub Date : 2016-11-15 DOI: 10.4291/wjgp.v7.i4.300

Y. Rhee, H. Ku, Hye-Ji Noh, Hyang-Hyun Cho, Hee-Kyong Kim, Jin-Chul Ahn

AIM To investigate the bactericidal effects of calcium chelated N-acetylneuraminic acid-glycomacropeptide (CaG-NANA) against Helicobacter pylori (H. pylori). METHODS For manufacture of CaG-NANA, calcium (Ca) was combined with glycomacropeptide (GMP) by chelating, and N-acetylneuraminic acid (NANA) was produced with Ca-GMP substrate by an enzymatic method. The final concentration of each component was 5% Ca, 7% NANA, 85% GMP, and 3% water. For in vitro study, various concentrations of CaG-NANA were investigated under the minimal inhibitory concentration (MIC). For in vivo study, CaG-NANA was administered orally for 3 wk after H. pylori infection. The levels of inflammatory cytokines in blood were analyzed by enzyme-linked immunosorbent assay and eradication of H. pylori was assessed by histological observation. RESULTS The time-kill curves showed a persistent decrease in cell numbers, which depended on the dose of CaG-NANA, and MIC of CaG-NANA against H. pylori was 0.5% in vitro. Histopathologic observation revealed no obvious inflammation or pathologic changes in the gastric mucosa in the CaG-NANA treatment group in vivo. The colonization of H. pylori was reduced after CaG-NANA treatment. The levels of interleukin (IL)-6, IL-1β, tumor necrosis factor-α, and IL-10 were also decreased by CaG-NANA. CONCLUSION CaG-NANA demonstrates effective anti-bactericidal activity against H. pylori both in vitro and in vivo.

目的研究钙螯合n -乙酰神经氨酸糖大肽(CaG-NANA)对幽门螺杆菌的杀菌作用。方法将钙(Ca)与糖大肽(GMP)螯合结合，以Ca-GMP为底物酶法合成n -乙酰神经氨酸(NANA)。各组分的最终浓度为5% Ca, 7% NANA, 85% GMP, 3%水。体外实验采用最低抑菌浓度(MIC)对不同浓度的CaG-NANA进行研究。在体内研究中，幽门螺杆菌感染后口服CaG-NANA 3周。采用酶联免疫吸附法分析血中炎症因子水平，采用组织学观察观察幽门螺杆菌根除情况。结果时间杀伤曲线显示细胞数量持续下降，且与剂量有关，体外对幽门螺杆菌的杀伤MIC为0.5%。组织病理学观察显示，CaG-NANA治疗组胃黏膜在体内无明显炎症及病理改变。经CaG-NANA处理后，幽门螺杆菌的定植量减少。白细胞介素(IL)-6、IL-1β、肿瘤坏死因子-α和IL-10的水平也被CaG-NANA降低。结论CaG-NANA对幽门螺杆菌具有较强的体外和体内抑菌活性。

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引用次数: 3