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Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry最新文献

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Determination of thermodynamic constants for agonist binding to muscarinic receptors from rat cerebral cortex. 激动剂与大鼠大脑皮层毒蕈碱受体结合的热力学常数测定。
Pub Date : 1987-03-01 DOI: 10.3891/acta.chem.scand.41b-0225
B Hedlund, T Bartfai
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引用次数: 1
Crystal and molecular structure of aspartame X HCl X 2H2O. 阿斯巴甜X HCl X 2H2O的晶体和分子结构。
Pub Date : 1987-02-01 DOI: 10.3891/acta.chem.scand.41b-0087
C H Görbitz

The crystal and molecular structure of the hydrochloride salt of the peptide sweetener aspartame (alpha-L-Asp-L-Phe methyl ester) has been determined at 120 K using 3877 reflections with I greater than 2.5 sigma I. Space group P2(1)2(1)2(1), cell dimensions a = 6.768(1), b = 9.796(1) and c = 26.520(3) A; final R factor 0.033. While the N-terminal L-Asp group in the structure of aspartame itself forms a six-membered ring with an intramolecular hydrogen bond between the carboxylate and the protonated amino terminus, the corresponding group in the hydrochloride adopts a completely different conformation with a weak intramolecular hydrogen bond between the carboxyl group and the N atom of the L-Phe residue. The L-Phe methyl ester moiety is rather similar in the two structures. Of the many possible conformations of aspartame, only one may be expected to function as a substrate at the receptor site for sweet taste, and a proposal is made for this active conformation.

用3877次反射(I大于2.5 σ I)在120 K下测定了肽甜味剂阿斯巴甜盐酸盐(α - l - asp - l - phe甲酯)的晶体和分子结构,空间群P2(1)2(1)2(1),细胞尺寸a = 6.768(1), b = 9.796(1), c = 26.520(3) a;最终R因子为0.033。在阿斯巴甜的结构中,N端L-Asp基团本身形成一个六元环,羧酸盐与质子化氨基端之间存在分子内氢键,而在盐酸中,相应的基团则采用完全不同的构象,羧基与L-Phe残基的N原子之间存在弱的分子内氢键。l -苯丙氨酸甲酯部分在两种结构中相当相似。在阿斯巴甜的许多可能的构象中,只有一种可能在甜味受体位点作为底物起作用,并提出了这种活性构象的建议。
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引用次数: 5
Similarities between soluble inorganic pyrophosphatase from yeast and some nucleotide-binding polypeptides. 酵母菌可溶性无机焦磷酸酶与某些核苷酸结合多肽的相似性。
Pub Date : 1987-02-01 DOI: 10.3891/acta.chem.scand.41b-0106
H Baltscheffsky, M Alauddin, G Falk, M Lundin
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引用次数: 8
Age-dependent changes in the levels of dolichol and dolichyl phosphates in human brain. 人类大脑中多酚和多酚磷酸酯水平的年龄依赖性变化。
Pub Date : 1987-02-01 DOI: 10.3891/acta.chem.scand.41b-0144
M Andersson, E L Appelkvist, K Kristensson, G Dallner
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引用次数: 8
Non-respiring rat liver mitochondria do not have a Ca2+/2H+ antiporter. 非呼吸大鼠肝脏线粒体不具有Ca2+/2H+反转运蛋白。
Pub Date : 1987-02-01 DOI: 10.3891/acta.chem.scand.41b-0079
N E Saris

Liver mitochondria take up Ca2+ by the Ca2+ uniporter, whereas at steady state efflux is believed to occur mainly by means of a ruthenium red-insensitive Ca2+/2H+ antiporter. The latter activity was studied in respiration-inhibited mitochondria in the presence of ruthenium red and was measured as Ca2+ uptake following acidification of the matrix by addition of nigericin, which catalyzes K+/H+ exchange. Ca2+ uptake was stimulated by protonophorous uncoupling agents and inhibited by increasing the concentration of ruthenium red. However, the rates were always smaller than those obtained by addition of valinomycin instead of nigericin. This indicates that under these conditions, Ca2+ fluxes are not mediated by a Ca2+/2H+ antiporter but by residual uniporter activity.

肝线粒体通过Ca2+单转运体吸收Ca2+,而在稳态下,外流被认为主要通过钌红不敏感的Ca2+/2H+反转运体发生。后一种活性在钌红存在下的呼吸抑制线粒体中进行了研究,并通过添加尼日利亚菌素酸化基质(催化K+/H+交换)后的Ca2+摄取来测量。原磷解偶联剂刺激Ca2+的摄取,增加钌红浓度抑制Ca2+的摄取。然而,用缬霉素代替尼日尔霉素的反应速率总是小于用缬霉素代替尼日尔霉素的反应速率。这表明在这些条件下,Ca2+通量不是由Ca2+/2H+反转运蛋白介导的,而是由残留的单转运蛋白活性介导的。
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引用次数: 8
Biosynthesis of ubiquinone in rat liver. 大鼠肝脏中泛醌的生物合成。
Pub Date : 1987-01-01 DOI: 10.3891/acta.chem.scand.41b-0070
A Kalén, E L Appelkvist, G Dallner
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引用次数: 9
Dolichol biosynthesis in rat liver peroxisomes. 大鼠肝脏过氧化物酶体的醇类生物合成。
Pub Date : 1987-01-01 DOI: 10.3891/acta.chem.scand.41b-0073
E L Appelkvist
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引用次数: 9
Biosynthesis of sterols and dolichol in human hepatomas. 人肝癌中甾醇和醇的生物合成。
Pub Date : 1987-01-01 DOI: 10.3891/acta.chem.scand.41b-0067
I Eggens
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引用次数: 7
Synthesis of O2'-methyluridine, O2'-methylcytidine, N4,O2'-dimethylcytidine and N4,N4,O2'-trimethylcytidine from a common intermediate. 共同中间体合成O2′-甲基尿苷、O2′-甲基胞苷、N4,O2′-二甲基胞苷和N4,N4,O2′-三甲基胞苷。
Pub Date : 1986-11-01 DOI: 10.3891/acta.chem.scand.40b-0826
A Nyilas, J Chattopadhyaya

A facile synthesis of the title compounds from a readily accessible precursor, 3',5'-O-bis-protected O4-(2-nitrophenyl)uridine, is described.

描述了一种由易于获得的前体3',5'- o -双保护的O4-(2-硝基苯基)尿嘧啶简便合成标题化合物的方法。
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引用次数: 22
Synthesis of an mRNA fragment of alanyl-tRNA synthetase gene in Escherichia coli using the 6-methyl-3-pyridyl group for protection of the imide functions of uridine and guanosine. 利用6-甲基-3-吡啶基在大肠杆菌中合成丙氨酰trna合成酶基因mRNA片段,保护尿苷和鸟苷的亚胺功能。
Pub Date : 1986-11-01 DOI: 10.3891/acta.chem.scand.40b-0817
C J Welch, X X Zhou, J Chattopadhyaya

The synthesis of 5'-GpGpUpGpU-3' is reported to demonstrate the synthetic use of the 6-methyl-3-pyridyl group for the protection of the O-4 and O-6 imide functions of uridine and guanosine, respectively. The 2'- and 5'-hydroxyl functions of the key intermediates were protected with two acid-labile groups: 3-methoxy-1,5-dicarbomethoxypentane-3-yl (MDMP) and 9-(4-octadecyloxyphenyl)xanthen-9-yl (C18Px), respectively. The internucleotide phosphotriesters were protected with 2-chlorophenyl and the 9-fluorenylmethyl group was employed for 3'-terminal phosphate protection.

5'-GpGpUpGpU-3'的合成证明了6-甲基-3-吡啶基的合成分别保护了尿苷和鸟苷的O-4和O-6亚胺功能。关键中间体的2′-和5′-羟基功能被两个酸不稳定基团分别保护:3-甲氧基-1,5-二甲氧基-氧戊烷-3-基(MDMP)和9-(4-十八烷基氧基)杂原-9-基(C18Px)。核苷酸间磷酸三酯用2-氯苯保护,9-芴基甲基用于3'端磷酸保护。
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引用次数: 8
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