Pub Date : 2022-03-03DOI: 10.1021/acsagscitech.1c00270
M. Sturme, J. P. van der Berg, L. Bouwman, A. de Schrijver, R. D. de Maagd, G. Kleter, Evy Battaglia-de Wilde
CRISPR-Cas-based genome editing allows for precise and targeted genetic modification of plants. Nevertheless, unintended off-target edits can arise that might confer risks when present in gene-edited food crops. Through an extensive literature review we gathered information on CRISPR-Cas off-target edits in plants. Most observed off-target changes were small insertions or deletions (1–22 bp) or nucleotide substitutions, and large deletions (>100 bp) were rare. One study detected the insertion of vector-derived DNA sequences, which is important considering the risk assessment of gene-edited plants. Off-target sites had few mismatches (1–3 nt) with the target sequence and were mainly located in protein-coding regions, often in target gene homologues. Off-targets edits were predominantly detected via biased analysis of predicted off-target sites instead of unbiased genome-wide analysis. CRISPR-Cas-edited plants showed lower off-target mutation frequencies than conventionally bred plants. This Review can aid discussions on the relevance of evaluating off-target modifications for risk assessment of CRISPR-Cas-edited plants.
{"title":"Occurrence and Nature of Off-Target Modifications by CRISPR-Cas Genome Editing in Plants","authors":"M. Sturme, J. P. van der Berg, L. Bouwman, A. de Schrijver, R. D. de Maagd, G. Kleter, Evy Battaglia-de Wilde","doi":"10.1021/acsagscitech.1c00270","DOIUrl":"https://doi.org/10.1021/acsagscitech.1c00270","url":null,"abstract":"CRISPR-Cas-based genome editing allows for precise and targeted genetic modification of plants. Nevertheless, unintended off-target edits can arise that might confer risks when present in gene-edited food crops. Through an extensive literature review we gathered information on CRISPR-Cas off-target edits in plants. Most observed off-target changes were small insertions or deletions (1–22 bp) or nucleotide substitutions, and large deletions (>100 bp) were rare. One study detected the insertion of vector-derived DNA sequences, which is important considering the risk assessment of gene-edited plants. Off-target sites had few mismatches (1–3 nt) with the target sequence and were mainly located in protein-coding regions, often in target gene homologues. Off-targets edits were predominantly detected via biased analysis of predicted off-target sites instead of unbiased genome-wide analysis. CRISPR-Cas-edited plants showed lower off-target mutation frequencies than conventionally bred plants. This Review can aid discussions on the relevance of evaluating off-target modifications for risk assessment of CRISPR-Cas-edited plants.","PeriodicalId":7007,"journal":{"name":"ACS Agricultural Science & Technology","volume":"69 1","pages":"192 - 201"},"PeriodicalIF":0.0,"publicationDate":"2022-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89207707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-22DOI: 10.1021/acsagscitech.2c00023
J. P. Walsh, C. P. Garnham, K. Yeung, M. Sumarah
{"title":"Ilyonectria Root Rot of Ginseng Is Attenuated via Enzymatic Degradation of the Extracellular Fe3+-Bound Siderophore N,N′,N″-Triacetylfusarinine C","authors":"J. P. Walsh, C. P. Garnham, K. Yeung, M. Sumarah","doi":"10.1021/acsagscitech.2c00023","DOIUrl":"https://doi.org/10.1021/acsagscitech.2c00023","url":null,"abstract":"","PeriodicalId":7007,"journal":{"name":"ACS Agricultural Science & Technology","volume":"323 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72551157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-14DOI: 10.1021/acsagscitech.1c00179
Abelardo López-Fernández, Isaac Lucas-Gómez, Brenda Karen González-Pérez, Alejandro Valdez Calderón, A. Rivas-Castillo, M. A. Gayosso-Morales
{"title":"Expired Analgesics as Domestic Germinators Applied to Lactuca sativa (Lettuce) and Daucus carota (Carrot) Species Proposed by Docking of the ETR-1 Site","authors":"Abelardo López-Fernández, Isaac Lucas-Gómez, Brenda Karen González-Pérez, Alejandro Valdez Calderón, A. Rivas-Castillo, M. A. Gayosso-Morales","doi":"10.1021/acsagscitech.1c00179","DOIUrl":"https://doi.org/10.1021/acsagscitech.1c00179","url":null,"abstract":"","PeriodicalId":7007,"journal":{"name":"ACS Agricultural Science & Technology","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73379315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-04DOI: 10.1021/acsagscitech.1c00276
A. Kaundal, D. Sandhu, Vishal Singh, M. Dueñas, B. Acharya, Bradley Nelson, J. F. Ferreira, A. Litt
{"title":"Transgenic Expression of Prunus persica Salt Overly Sensitive 2 (PpSOS2) in the atsos2 Mutant Imparts Salt Tolerance in Arabidopsis","authors":"A. Kaundal, D. Sandhu, Vishal Singh, M. Dueñas, B. Acharya, Bradley Nelson, J. F. Ferreira, A. Litt","doi":"10.1021/acsagscitech.1c00276","DOIUrl":"https://doi.org/10.1021/acsagscitech.1c00276","url":null,"abstract":"","PeriodicalId":7007,"journal":{"name":"ACS Agricultural Science & Technology","volume":"130 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75978021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-03DOI: 10.1021/acsagscitech.1c00237
Marjana Yeasmin, D. Lamb, G. Choppala, M. Rahman
{"title":"Selenium Accumulation and Speciation in Chickpea (Cicer arietinum) Impacted by S in Soils: Potential for Biofortification","authors":"Marjana Yeasmin, D. Lamb, G. Choppala, M. Rahman","doi":"10.1021/acsagscitech.1c00237","DOIUrl":"https://doi.org/10.1021/acsagscitech.1c00237","url":null,"abstract":"","PeriodicalId":7007,"journal":{"name":"ACS Agricultural Science & Technology","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88079881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-26DOI: 10.1021/acsagscitech.1c00279
M. R. Maximiano, O. Franco
{"title":"CRISPR/Cas: The New Frontier in Plant Improvement","authors":"M. R. Maximiano, O. Franco","doi":"10.1021/acsagscitech.1c00279","DOIUrl":"https://doi.org/10.1021/acsagscitech.1c00279","url":null,"abstract":"","PeriodicalId":7007,"journal":{"name":"ACS Agricultural Science & Technology","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73485044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-21DOI: 10.1021/acsagscitech.1c00074
O. A. Osipitan, M. Mesgaran, B. Hanson, J. Fernández-Bayo
{"title":"Leveraging Tomato Crop Residues and Pomace for Biosolarization to Deplete Weed Seedbank in Tomato Fields","authors":"O. A. Osipitan, M. Mesgaran, B. Hanson, J. Fernández-Bayo","doi":"10.1021/acsagscitech.1c00074","DOIUrl":"https://doi.org/10.1021/acsagscitech.1c00074","url":null,"abstract":"","PeriodicalId":7007,"journal":{"name":"ACS Agricultural Science & Technology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77085148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-11DOI: 10.1021/acsagscitech.1c00160
O. Sacko, Xu Feng, J. R. Morris, R. M. Council-Troche, Sandeep Kumar, J. Lee
{"title":"Sustainable Green Chemistry: Water-Soluble Ozonized Biochar Molecules To Unlock Phosphorus from Insoluble Phosphate Materials","authors":"O. Sacko, Xu Feng, J. R. Morris, R. M. Council-Troche, Sandeep Kumar, J. Lee","doi":"10.1021/acsagscitech.1c00160","DOIUrl":"https://doi.org/10.1021/acsagscitech.1c00160","url":null,"abstract":"","PeriodicalId":7007,"journal":{"name":"ACS Agricultural Science & Technology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87439764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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