Pub Date : 2024-08-01Epub Date: 2024-06-10DOI: 10.1177/09645284241256669
Chao Ke, Shengtao Shan, Yan Tan, Yang Cao, Zhengrong Xie, Senjie Shi, Jiang Pan, Wei Zhang
Background: To date, there is no effective treatment for Alzheimer's disease (AD), a progressive neurodegenerative disorder that is increasing in prevalence worldwide. The objective of this review was to summarize the core targets and signaling pathways involved in acupuncture treatment for AD.
Methods: We reviewed numerous signaling pathways, including mammalian target of rapamycin (mTOR), phosphatidylinositol 3-kinase-protein kinase B (PI3 K/Akt), adenosine monophosphate-activated protein kinase (AMPK), mitogen-activated protein kinase (MAPK), nuclear factor (NF)-kB, p53, Wnt, nitric oxide (NO), Janus kinase / signal transducer and activator of transcription (JAK/ STAT), RhoA/ROCK (Rho-associated protein kinase) and Ca2+/ calmodulin-dependent protein kinase II (CaMKII) / cyclic adenosine monophosphate-response element-binding protein (CREB). The relevant data were obtained from PubMed, EMBASE, Web of Science, China National Knowledge Infrastructure (CNKI) and Wanfang databases.
Results: In summary, the effects of acupuncture are mediated by multiple targets and pathways. Furthermore, acupuncture can improve pathological changes associated with AD (such as abnormal deposition of amyloid (A)β, tau hyperphosphorylation, synaptic dysfunction and neuronal apoptosis) through multiple signaling pathways.
Conclusion: Overall, our findings provide a basis for future research into the effects of acupuncture on AD.
背景:阿尔茨海默病(AD)是一种进行性神经退行性疾病,在世界范围内发病率不断上升。本综述旨在总结针灸治疗阿尔茨海默病的核心靶点和信号通路:我们回顾了众多信号通路,包括哺乳动物雷帕霉素靶标(mTOR)、磷脂酰肌醇3-激酶-蛋白激酶B(PI3 K/Akt)、单磷酸腺苷激活蛋白激酶(AMPK)、丝裂原活化蛋白激酶(MAPK)、核因子(NF)-kB、p53、Wnt、一氧化氮(NO)、Janus 激酶/转录信号转导和激活因子(JAK/ STAT)、RhoA/ROCK(Rho 相关蛋白激酶)和 Ca2+/ 钙调素依赖性蛋白激酶 II(CaMKII)/环磷酸腺苷反应元件结合蛋白(CREB)。相关数据来自PubMed、EMBASE、Web of Science、中国知网(CNKI)和万方数据库:总之,针灸的作用是由多个靶点和途径介导的。此外,针灸还能通过多种信号通路改善与AD相关的病理改变(如淀粉样蛋白(A)β异常沉积、tau过度磷酸化、突触功能障碍和神经元凋亡):总之,我们的研究结果为今后研究针灸对AD的影响奠定了基础。
{"title":"Signaling pathways in the treatment of Alzheimer's disease with acupuncture: a narrative review.","authors":"Chao Ke, Shengtao Shan, Yan Tan, Yang Cao, Zhengrong Xie, Senjie Shi, Jiang Pan, Wei Zhang","doi":"10.1177/09645284241256669","DOIUrl":"10.1177/09645284241256669","url":null,"abstract":"<p><strong>Background: </strong>To date, there is no effective treatment for Alzheimer's disease (AD), a progressive neurodegenerative disorder that is increasing in prevalence worldwide. The objective of this review was to summarize the core targets and signaling pathways involved in acupuncture treatment for AD.</p><p><strong>Methods: </strong>We reviewed numerous signaling pathways, including mammalian target of rapamycin (mTOR), phosphatidylinositol 3-kinase-protein kinase B (PI3 K/Akt), adenosine monophosphate-activated protein kinase (AMPK), mitogen-activated protein kinase (MAPK), nuclear factor (NF)-kB, p53, Wnt, nitric oxide (NO), Janus kinase / signal transducer and activator of transcription (JAK/ STAT), RhoA/ROCK (Rho-associated protein kinase) and Ca<sup>2+</sup>/ calmodulin-dependent protein kinase II (CaMKII) / cyclic adenosine monophosphate-response element-binding protein (CREB). The relevant data were obtained from PubMed, EMBASE, Web of Science, China National Knowledge Infrastructure (CNKI) and Wanfang databases.</p><p><strong>Results: </strong>In summary, the effects of acupuncture are mediated by multiple targets and pathways. Furthermore, acupuncture can improve pathological changes associated with AD (such as abnormal deposition of amyloid (A)β, tau hyperphosphorylation, synaptic dysfunction and neuronal apoptosis) through multiple signaling pathways.</p><p><strong>Conclusion: </strong>Overall, our findings provide a basis for future research into the effects of acupuncture on AD.</p>","PeriodicalId":7257,"journal":{"name":"Acupuncture in Medicine","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141299750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-01Epub Date: 2024-06-07DOI: 10.1177/09645284241256670
Amit Kumar
{"title":"Electroacupuncture for post-COVID proctodynia: a case report.","authors":"Amit Kumar","doi":"10.1177/09645284241256670","DOIUrl":"10.1177/09645284241256670","url":null,"abstract":"","PeriodicalId":7257,"journal":{"name":"Acupuncture in Medicine","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141282670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of acupuncture on corrugator muscle activity in temporomandibular joint disorder: a case report.","authors":"Takahiro Yamada, Hiroyoshi Yajima, Miho Takayama, Morihiro Nasu, Konomi Imanishi, Judith M Schlaeger, Nobuari Takakura","doi":"10.1177/09645284241262049","DOIUrl":"10.1177/09645284241262049","url":null,"abstract":"","PeriodicalId":7257,"journal":{"name":"Acupuncture in Medicine","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141417257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-01Epub Date: 2024-05-09DOI: 10.1177/09645284241249197
Mark D Sodders, Shyam J Deshpande, Jeffery A Dusek, Ryan Bradley, Monica S Vavilala
Background: Acupuncture is a promising treatment for common symptoms after traumatic brain injury (TBI). Our objectives were to explore knowledge, attitudes and beliefs about acupuncture, identify health service needs and assess the perceived feasibility of weekly acupuncture visits among individuals with TBI.
Methods: We surveyed adults 18 years of age and older with TBI who received care at the University of Washington. Respondents were asked to complete 143 questions regarding acupuncture knowledge, attitudes and beliefs, injury-related symptoms and comorbidities, and to describe their interest in weekly acupuncture.
Results: Respondents (n = 136) reported a high degree of knowledge about acupuncture as a component of Traditional Chinese Medicine, needle use and safety, but were less knowledgeable regarding that the fact that most conditions require multiple acupuncture treatments to achieve optimal therapeutic benefit. Respondents were comfortable talking with healthcare providers about acupuncture (63.4%), open to acupuncture concurrent with conventional treatments (80.6%) and identified lack of insurance coverage as a barrier (50.8%). Beliefs varied, but respondents were generally receptive to using acupuncture as therapy. Unsurprisingly, respondents with a history of acupuncture (n = 60) had more acupuncture knowledge than those without such a history (n = 66) and were more likely to pursue acupuncture without insurance (60%), for serious health conditions (63.3%) or alongside conventional medical therapy (85.0%). Half of all respondents expressed interest in participating in weekly acupuncture for up to 12 months and would expect almost a 50% improvement in symptoms by participating.
Conclusion: Adults with TBI were receptive and interested in participating in weekly acupuncture to address health concerns. These results provide support for exploring the integration of acupuncture into the care of individuals with TBI.
{"title":"Acupuncture knowledge, attitudes, beliefs and health service needs after traumatic brain injury: a cross-sectional survey.","authors":"Mark D Sodders, Shyam J Deshpande, Jeffery A Dusek, Ryan Bradley, Monica S Vavilala","doi":"10.1177/09645284241249197","DOIUrl":"10.1177/09645284241249197","url":null,"abstract":"<p><strong>Background: </strong>Acupuncture is a promising treatment for common symptoms after traumatic brain injury (TBI). Our objectives were to explore knowledge, attitudes and beliefs about acupuncture, identify health service needs and assess the perceived feasibility of weekly acupuncture visits among individuals with TBI.</p><p><strong>Methods: </strong>We surveyed adults 18 years of age and older with TBI who received care at the University of Washington. Respondents were asked to complete 143 questions regarding acupuncture knowledge, attitudes and beliefs, injury-related symptoms and comorbidities, and to describe their interest in weekly acupuncture.</p><p><strong>Results: </strong>Respondents (n = 136) reported a high degree of knowledge about acupuncture as a component of Traditional Chinese Medicine, needle use and safety, but were less knowledgeable regarding that the fact that most conditions require multiple acupuncture treatments to achieve optimal therapeutic benefit. Respondents were comfortable talking with healthcare providers about acupuncture (63.4%), open to acupuncture concurrent with conventional treatments (80.6%) and identified lack of insurance coverage as a barrier (50.8%). Beliefs varied, but respondents were generally receptive to using acupuncture as therapy. Unsurprisingly, respondents with a history of acupuncture (n = 60) had more acupuncture knowledge than those without such a history (n = 66) and were more likely to pursue acupuncture without insurance (60%), for serious health conditions (63.3%) or alongside conventional medical therapy (85.0%). Half of all respondents expressed interest in participating in weekly acupuncture for up to 12 months and would expect almost a 50% improvement in symptoms by participating.</p><p><strong>Conclusion: </strong>Adults with TBI were receptive and interested in participating in weekly acupuncture to address health concerns. These results provide support for exploring the integration of acupuncture into the care of individuals with TBI.</p>","PeriodicalId":7257,"journal":{"name":"Acupuncture in Medicine","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140891087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-31DOI: 10.1177/09645284241265869
Jing Peng, Mi Zou, Manwa L Ng, Jie Tan, Juan Xiang
{"title":"Nonsurgical treatment of bilateral vocal fold paralysis: a case study of voice training combined with acupuncture.","authors":"Jing Peng, Mi Zou, Manwa L Ng, Jie Tan, Juan Xiang","doi":"10.1177/09645284241265869","DOIUrl":"https://doi.org/10.1177/09645284241265869","url":null,"abstract":"","PeriodicalId":7257,"journal":{"name":"Acupuncture in Medicine","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141858673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-28DOI: 10.1177/09645284241265872
Jing Liu, Jun Zhou, Jinlin Wang, Xiarong Huang, Mengjian Qu, Ying Liao, Guanghua Sun, Peirui Zhong, Jinqu Tan, Zhilu Sun
Objectives: Osteoporosis is widely regarded as a typical aged-related disease caused by impaired bone remodeling. This research was designed to explore the protective effects of electroacupuncture (EA) on senile osteoporosis in a rat model and investigate the underlying mechanisms.
Methods: Three-month-old rats were randomly selected as the youth group, and 24-month-old rats were randomly assigned to the elderly and EA groups. Rats in the EA group received 30 min of EA at bilateral SP10, ST36, K13 and GB34 daily, 5 days a week for 8 weeks. Bone mineral density (BMD), microstructure of the bone tissue, bone turnover biomarkers and expression level of autophagy-related proteins were detected.
Results: Compared with the elderly group, EA treatment significantly increased BMD of the femur and ameliorated the microstructure. EA treatment increased trabecular bone volume ratio (= bone volume / total volume [BV/TV]) and trabecular number (Tb.N) and decreased trabecular separation (Tb.Sp) in senile osteoporosis rats. Compared with the elderly group, the serum N-terminal telopeptide of type I collagen (NTX1) level in the EA group was lower, and the serum procollagen type I N-terminal propeptide (PINP) concentration was higher. In addition, the expression of Beclin 1, microtubule-associated protein I light chain 3 (LC3B) and P62 was inhibited in the senile osteoporosis rats after EA treatment.
Conclusions: EA can effectively alleviate aging-related bone loss and improve the microstructure of bone tissue in senile osteoporosis rats, and the regulation of autophagy might be one of the important mechanisms.
目的:骨质疏松症被广泛认为是一种典型的老年性疾病,是由骨重塑障碍引起的。本研究旨在探讨电针(EA)对老年性骨质疏松症大鼠模型的保护作用及其内在机制:方法:随机选取 3 个月大的大鼠为青年组,24 个月大的大鼠为老年组和 EA 组。EA 组大鼠每天在双侧 SP10、ST36、K13 和 GB34 处接受 30 分钟的 EA,每周 5 天,连续 8 周。结果显示:与老年组相比,EA治疗组大鼠的骨矿物质密度(BMD)、骨组织的微观结构、骨转换生物标志物和自噬相关蛋白的表达水平均有所提高:结果:与老年组相比,EA治疗显著增加了股骨的骨密度,改善了骨组织的微观结构。EA治疗可增加老年骨质疏松症大鼠的骨小梁体积比(=骨体积/总体积[BV/TV])和骨小梁数量(Tb.N),降低骨小梁分离度(Tb.Sp)。与老年组相比,EA 组血清中 I 型胶原 N 端端肽(NTX1)水平较低,血清中 I 型胶原 N 端端肽(PINP)浓度较高。此外,EA治疗后,老年性骨质疏松症大鼠Beclin 1、微管相关蛋白I轻链3(LC3B)和P62的表达均受到抑制:结论:EA能有效缓解衰老相关性骨质流失,改善老年性骨质疏松症大鼠骨组织的微观结构,自噬调节可能是其重要机制之一。
{"title":"Electroacupuncture ameliorates senile osteoporosis by promoting bone remodeling and regulating autophagy.","authors":"Jing Liu, Jun Zhou, Jinlin Wang, Xiarong Huang, Mengjian Qu, Ying Liao, Guanghua Sun, Peirui Zhong, Jinqu Tan, Zhilu Sun","doi":"10.1177/09645284241265872","DOIUrl":"https://doi.org/10.1177/09645284241265872","url":null,"abstract":"<p><strong>Objectives: </strong>Osteoporosis is widely regarded as a typical aged-related disease caused by impaired bone remodeling. This research was designed to explore the protective effects of electroacupuncture (EA) on senile osteoporosis in a rat model and investigate the underlying mechanisms.</p><p><strong>Methods: </strong>Three-month-old rats were randomly selected as the youth group, and 24-month-old rats were randomly assigned to the elderly and EA groups. Rats in the EA group received 30 min of EA at bilateral SP10, ST36, K13 and GB34 daily, 5 days a week for 8 weeks. Bone mineral density (BMD), microstructure of the bone tissue, bone turnover biomarkers and expression level of autophagy-related proteins were detected.</p><p><strong>Results: </strong>Compared with the elderly group, EA treatment significantly increased BMD of the femur and ameliorated the microstructure. EA treatment increased trabecular bone volume ratio (= bone volume / total volume [BV/TV]) and trabecular number (Tb.N) and decreased trabecular separation (Tb.Sp) in senile osteoporosis rats. Compared with the elderly group, the serum N-terminal telopeptide of type I collagen (NTX1) level in the EA group was lower, and the serum procollagen type I N-terminal propeptide (PINP) concentration was higher. In addition, the expression of Beclin 1, microtubule-associated protein I light chain 3 (LC3B) and P62 was inhibited in the senile osteoporosis rats after EA treatment.</p><p><strong>Conclusions: </strong>EA can effectively alleviate aging-related bone loss and improve the microstructure of bone tissue in senile osteoporosis rats, and the regulation of autophagy might be one of the important mechanisms.</p>","PeriodicalId":7257,"journal":{"name":"Acupuncture in Medicine","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141787038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01Epub Date: 2023-12-20DOI: 10.1177/09645284231210576
Zhibin Fu, Han Wang, Xin Guo, Yizhuan Huang
{"title":"Acupuncture for functional ankle instability: a case report.","authors":"Zhibin Fu, Han Wang, Xin Guo, Yizhuan Huang","doi":"10.1177/09645284231210576","DOIUrl":"10.1177/09645284231210576","url":null,"abstract":"","PeriodicalId":7257,"journal":{"name":"Acupuncture in Medicine","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138827592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01Epub Date: 2024-05-30DOI: 10.1177/09645284241248466
Hao Gao, Liyue Lu, Lili Li, Zhiyu Yin, Wenqing Ruan, Yinghua Zou, He Li, Yalan Zhou, Xing Li, Yongqiang Wang, Jiangang Song, Wenting Chen, Jian Wang
Background: Electroacupuncture (EA) has been reported to improve intestinal motility in mice with postoperative ileus (POI). Previous studies, however, have yielded heterogeneous results regarding the effect of EA on POI.
Methods: Herein, a POI mouse model was constructed by intestinal manipulation. To evaluate the effect of EA treatment on colonic transit, the levels of inflammatory markers (macrophage inflammatory protein (MIP)-1α, interleukin (IL)-1β, IL-6, monocyte chemotactic protein (MCP)-1 and intercellular adhesion molecule (ICAM)-1) were detected by enzyme-linked immunosorbent assay (ELISA); immune cell infiltration was detected by immunohistochemical staining of myeloperoxidase (MPO), ectodysplasin (ED)-1 and ED-2, and the percentage of CD4+ interferon (IFN)-γ+ Th1 cells and IFN-γ secretion levels were determined. Activated Th1 cells and pentoxifylline, a cell differentiation inhibitor, were used to assess the role of Th1 cells in EA treatment of POI. Neostigmine administration and unilateral vagotomy were performed to confirm whether the effects of EA treatment on Th1 cells were mediated by the vagus nerve (VN).
Results: The results revealed that EA treatment at ST36 improved POI, as indicated by a decreased level of inflammatory-related markers and immune cell infiltration and shortened colonic transit time. The activated Th1 cells abolished the effects of EA treatment on POI. The effects of EA treatment on POI were enhanced by stimulation of the VN along with a decreased level of Th1 cells, but these effects were abolished by vagotomy along with an increased percentage of Th1 cells; this result indicates that the VN mediates the role of Th1 cells in the effects of EA treatment of POI.
Conclusion: Our findings showed that the effects of EA treatment of POI were mainly mediated by Th1 cells through the stimulation of the VN and inhibition of the inflammatory response.
背景:有报道称电针(EA)可改善术后回肠梗阻(POI)小鼠的肠蠕动。方法:本文通过肠道操作建立了 POI 小鼠模型。为了评估 EA 治疗对结肠转运的影响,用酶联免疫吸附试验(ELISA)检测了炎症标志物(巨噬细胞炎症蛋白(MIP)-1α、白细胞介素(IL)-1β、IL-6、单核细胞趋化蛋白(MCP)-1 和细胞间粘附分子(ICAM)-1)的水平;免疫组织化学染色法检测髓过氧化物酶(MPO)、外胚层蛋白(ED)-1 和 ED-2,并测定 CD4+ 干扰素(IFN)-γ+ Th1 细胞的百分比和 IFN-γ 的分泌水平。活化的 Th1 细胞和细胞分化抑制剂喷托非林被用来评估 Th1 细胞在 EA 治疗 POI 中的作用。为了证实 EA 治疗对 Th1 细胞的影响是否由迷走神经(VN)介导,还进行了新斯的明给药和单侧迷走神经切断术:结果:结果表明,在 ST36 段进行 EA 治疗可改善 POI,这表现在炎症相关标记物和免疫细胞浸润水平降低以及结肠转运时间缩短。活化的 Th1 细胞消除了 EA 处理对 POI 的影响。刺激迷走神经可增强 EA 治疗对 POI 的影响,同时降低 Th1 细胞的水平,但迷走神经切断可消除这些影响,同时增加 Th1 细胞的比例;这一结果表明迷走神经在 EA 治疗对 POI 的影响中介导了 Th1 细胞的作用:我们的研究结果表明,EA 治疗 POI 的效果主要由 Th1 细胞通过刺激 VN 和抑制炎症反应介导。
{"title":"Electroacupuncture treatment improves postoperative ileus by inhibiting the Th1 cell-mediated inflammatory response through the vagus nerve.","authors":"Hao Gao, Liyue Lu, Lili Li, Zhiyu Yin, Wenqing Ruan, Yinghua Zou, He Li, Yalan Zhou, Xing Li, Yongqiang Wang, Jiangang Song, Wenting Chen, Jian Wang","doi":"10.1177/09645284241248466","DOIUrl":"10.1177/09645284241248466","url":null,"abstract":"<p><strong>Background: </strong>Electroacupuncture (EA) has been reported to improve intestinal motility in mice with postoperative ileus (POI). Previous studies, however, have yielded heterogeneous results regarding the effect of EA on POI.</p><p><strong>Methods: </strong>Herein, a POI mouse model was constructed by intestinal manipulation. To evaluate the effect of EA treatment on colonic transit, the levels of inflammatory markers (macrophage inflammatory protein (MIP)-1α, interleukin (IL)-1β, IL-6, monocyte chemotactic protein (MCP)-1 and intercellular adhesion molecule (ICAM)-1) were detected by enzyme-linked immunosorbent assay (ELISA); immune cell infiltration was detected by immunohistochemical staining of myeloperoxidase (MPO), ectodysplasin (ED)-1 and ED-2, and the percentage of CD4<sup>+</sup> interferon (IFN)-γ<sup>+</sup> Th1 cells and IFN-γ secretion levels were determined. Activated Th1 cells and pentoxifylline, a cell differentiation inhibitor, were used to assess the role of Th1 cells in EA treatment of POI. Neostigmine administration and unilateral vagotomy were performed to confirm whether the effects of EA treatment on Th1 cells were mediated by the vagus nerve (VN).</p><p><strong>Results: </strong>The results revealed that EA treatment at ST36 improved POI, as indicated by a decreased level of inflammatory-related markers and immune cell infiltration and shortened colonic transit time. The activated Th1 cells abolished the effects of EA treatment on POI. The effects of EA treatment on POI were enhanced by stimulation of the VN along with a decreased level of Th1 cells, but these effects were abolished by vagotomy along with an increased percentage of Th1 cells; this result indicates that the VN mediates the role of Th1 cells in the effects of EA treatment of POI.</p><p><strong>Conclusion: </strong>Our findings showed that the effects of EA treatment of POI were mainly mediated by Th1 cells through the stimulation of the VN and inhibition of the inflammatory response.</p>","PeriodicalId":7257,"journal":{"name":"Acupuncture in Medicine","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141174164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01Epub Date: 2023-12-27DOI: 10.1177/09645284231210880
Wendi Dong, Xiaxia Jin, Shan Mo
{"title":"Acupuncture for androgenetic alopecia: a report of two cases.","authors":"Wendi Dong, Xiaxia Jin, Shan Mo","doi":"10.1177/09645284231210880","DOIUrl":"10.1177/09645284231210880","url":null,"abstract":"","PeriodicalId":7257,"journal":{"name":"Acupuncture in Medicine","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139039361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Oxidative stress and inflammatory responses play essential roles in cerebral ischemia/reperfusion (I/R) injury. Electroacupuncture (EA) is widely used as a rehabilitation method for stroke in China; however, the underlying mechanism of action remains unclear. Peroxisome proliferator-activated receptor gamma (PPAR-γ) has been reported to impact anti-inflammatory and anti-oxidative effects.
Objective: This study investigated the role of PPAR-γ in EA-mediated effects and aimed to illuminate its possible mechanisms in cerebral I/R.
Methods: In this study, male Sprague-Dawley (SD) rats with middle cerebral artery occlusion/reperfusion (MCAO/R) injury were treated with EA at LI11 and ST36 for 30 min daily after MCAO/R for seven consecutive days. The neuroprotective effects of EA were measured by neurobehavioral evaluation, triphenyltetrazolium chloride staining, hematoxylin-eosin staining and transmission electron microscopy. Oxidative stress, inflammatory factors, neural apoptosis and microglial activation were examined by enzyme-linked immunosorbent assay, immunofluorescence and reverse transcriptase polymerase chain reaction. Western blotting was used to assess PPAR-γ-mediated signaling.
Results: We found that EA significantly alleviated cerebral I/R-induced infarct volume, decreased neurological scores and inhibited I/R-induced oxidative stress, inflammatory responses and microglial activation. EA also increased PPAR-γ protein expression. Furthermore, the protective effects of EA were reversed by injection of the PPAR-γ antagonist T0070907.
Conclusion: EA attenuates cerebral I/R injury by regulating oxidative stress, neuronal death and neuroinflammation via stimulation of PPAR-γ.
背景:氧化应激和炎症反应在脑缺血再灌注(I/R)损伤中起着至关重要的作用。电针(EA)在中国被广泛用作脑卒中的康复治疗方法,但其潜在的作用机制仍不清楚。据报道,过氧化物酶体增殖激活受体γ(PPAR-γ)具有抗炎和抗氧化作用:本研究探讨了 PPAR-γ 在 EA 介导的效应中的作用,并旨在阐明其在脑 I/R 中的可能机制:本研究以雄性Sprague-Dawley(SD)大脑中动脉闭塞/再灌注(MCAO/R)损伤大鼠为研究对象,在MCAO/R后连续7天每天用LI11和ST36浓度的EA治疗30 min。通过神经行为评估、三苯基氯化四氮唑染色、苏木精-伊红染色和透射电子显微镜检测了EA的神经保护作用。通过酶联免疫吸附试验、免疫荧光和逆转录酶聚合酶链反应检测氧化应激、炎症因子、神经凋亡和小胶质细胞活化。用 Western 印迹法评估 PPAR-γ 介导的信号传导:结果:我们发现 EA 能明显减轻 I/R 引发的脑梗死体积,降低神经系统评分,抑制 I/R 引发的氧化应激、炎症反应和小胶质细胞活化。EA 还能增加 PPAR-γ 蛋白的表达。此外,注射 PPAR-γ 拮抗剂 T0070907 会逆转 EA 的保护作用:结论:EA通过刺激PPAR-γ调节氧化应激、神经元死亡和神经炎症,从而减轻脑I/R损伤。
{"title":"Electroacupuncture attenuates cerebral ischemia/reperfusion injury by regulating oxidative stress, neuronal death and neuroinflammation via stimulation of PPAR-γ.","authors":"Nanfang Cheng, Xinyuan Cheng, Feng Tan, Yangui Liang, Lihong Xu, Jian Wang, Jiuqing Tan","doi":"10.1177/09645284231211600","DOIUrl":"10.1177/09645284231211600","url":null,"abstract":"<p><strong>Background: </strong>Oxidative stress and inflammatory responses play essential roles in cerebral ischemia/reperfusion (I/R) injury. Electroacupuncture (EA) is widely used as a rehabilitation method for stroke in China; however, the underlying mechanism of action remains unclear. Peroxisome proliferator-activated receptor gamma (PPAR-γ) has been reported to impact anti-inflammatory and anti-oxidative effects.</p><p><strong>Objective: </strong>This study investigated the role of PPAR-γ in EA-mediated effects and aimed to illuminate its possible mechanisms in cerebral I/R.</p><p><strong>Methods: </strong>In this study, male Sprague-Dawley (SD) rats with middle cerebral artery occlusion/reperfusion (MCAO/R) injury were treated with EA at LI11 and ST36 for 30 min daily after MCAO/R for seven consecutive days. The neuroprotective effects of EA were measured by neurobehavioral evaluation, triphenyltetrazolium chloride staining, hematoxylin-eosin staining and transmission electron microscopy. Oxidative stress, inflammatory factors, neural apoptosis and microglial activation were examined by enzyme-linked immunosorbent assay, immunofluorescence and reverse transcriptase polymerase chain reaction. Western blotting was used to assess PPAR-γ-mediated signaling.</p><p><strong>Results: </strong>We found that EA significantly alleviated cerebral I/R-induced infarct volume, decreased neurological scores and inhibited I/R-induced oxidative stress, inflammatory responses and microglial activation. EA also increased PPAR-γ protein expression. Furthermore, the protective effects of EA were reversed by injection of the PPAR-γ antagonist T0070907.</p><p><strong>Conclusion: </strong>EA attenuates cerebral I/R injury by regulating oxidative stress, neuronal death and neuroinflammation via stimulation of PPAR-γ.</p>","PeriodicalId":7257,"journal":{"name":"Acupuncture in Medicine","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139728728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}