International journal of stem cell research and therapy最新文献

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Role of CD9 Sensing, AI, and Exosomes in Cellular Communication of Cancer. CD9传感、AI和外泌体在肿瘤细胞通讯中的作用。

International journal of stem cell research and therapy

Pub Date : 2023-01-01 DOI: 10.23937/2469-570X/1410079

Neda Baghban, Sai Priyanka Kodam, Mujib Ullah

Exosomes are small membrane-bound vesicles that are released by various types of cells, including cancer cells, and play a role in intercellular communication. CD9 is a protein that is involved in cell signaling and adhesion. It is found on the surface of various cells, including cancer cells, and has been implicated in the communication between cancer cells and their microenvironment. Exosomes are small membrane-bound vesicles that are released by cells and contain various bioactive molecules, such as proteins, lipids, and nucleic acids. Exosomes have been shown to play a role in intercellular communication, and they have been implicated in the progression of cancer. There is evidence to suggest that CD9 is involved in the packaging and release of exosomes by cancer cells. CD9 has been shown to be important for the formation of tetraspanin-enriched microdomains (TEMs) on the surface of exosomes. These TEMs are thought to be important for the sorting and packaging of specific molecules into exosomes. In summary, CD9 appears to play an important role in the communication between cancer cells and their microenvironment via exosomes. The precise mechanisms by which CD9 mediates this communication are still being investigated, but the involvement of CD9 in exosome packaging and uptake suggests that it may be a promising target for the development of novel cancer therapies. Furthermore, CD9 has been shown to be involved in the uptake of exosomes by recipient cells. For example, studies have shown that CD9-positive exosomes released by cancer cells can be taken up by other cancer cells, leading to the transfer of oncogenic molecules and the promotion of cancer progression.

外泌体是小的膜结合囊泡，由包括癌细胞在内的各种类型的细胞释放，并在细胞间通讯中发挥作用。CD9是一种参与细胞信号传导和粘附的蛋白质。它存在于包括癌细胞在内的各种细胞表面，并与癌细胞与其微环境之间的通讯有关。外泌体是由细胞释放的膜结合的小泡，含有各种生物活性分子，如蛋白质、脂质和核酸。外泌体已被证明在细胞间通讯中发挥作用，并且它们与癌症的进展有关。有证据表明，CD9参与了癌细胞外泌体的包装和释放。CD9已被证明在外泌体表面形成富含四联蛋白的微结构域(TEMs)是重要的。这些tem被认为对外泌体中特定分子的分类和包装很重要。综上所述，CD9似乎在癌细胞通过外泌体与其微环境之间的通讯中发挥了重要作用。CD9介导这种通讯的确切机制仍在研究中，但CD9参与外泌体的包装和摄取表明，它可能是开发新型癌症疗法的一个有希望的靶点。此外，CD9已被证明参与受体细胞对外泌体的摄取。例如，研究表明，癌细胞释放的cd9阳性外泌体可被其他癌细胞吸收，导致致癌分子转移，促进癌症进展。

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引用次数: 1

Regulatory Prospects of Clinical Trials with Stem Cells in the United Arab Emirates 阿拉伯联合酋长国干细胞临床试验的监管前景

International journal of stem cell research and therapy

Pub Date : 2021-12-31 DOI: 10.23937/2469-570x/1410073

Y. Castillo-Aleman, Y. Ventura-Carmenate, R. A. Rivero-Jiménez, Antonio Bencomo-Hernández

Health research and clinical trials are driving the development of valuable medical knowledge. Many types of clinical research have been conducted in the United Arab Emirates (UAE) by governmental and private health providers in recent years, and a growing number of publications and UAE-based clinical trials have been registered worldwide. However, there are wide differences between the Emirates regarding the policies governing emerging stem cell therapies. This article aims to discuss the regulatory framework for conducting clinical trials involving stem cell therapies in the UAE. UAE-based clinical trials with stem cells were searched in international registries and national databases. Two health authorities are leading regulatory affairs on stem cells and regenerative medicine: The Department of Health - Abu Dhabi and Dubai Health Authority, while the public databases revealed 7 studies that investigated stem cells in the UAE. We recommend increasing the inter-organizational relationships between health authorities and regulated parties.

健康研究和临床试验正在推动有价值的医学知识的发展。近年来，阿拉伯联合酋长国(阿联酋)的政府和私营保健提供者进行了许多类型的临床研究，越来越多的出版物和以阿联酋为基础的临床试验已在世界范围内注册。然而，在管理新兴干细胞疗法的政策方面，阿联酋之间存在很大差异。本文旨在讨论在阿联酋开展涉及干细胞治疗的临床试验的监管框架。在国际注册和国家数据库中检索了以阿联酋为基础的干细胞临床试验。两个卫生当局正在领导干细胞和再生医学的监管事务:阿布扎比卫生部和迪拜卫生局，而公共数据库显示了在阿联酋调查干细胞的7项研究。我们建议加强卫生当局与受管制方之间的组织间关系。

引用次数: 0

Clinical Relevance of RNA Editing to Early Detection of Cancer in Human. RNA编辑在人类癌症早期检测中的临床意义。

International journal of stem cell research and therapy

Pub Date : 2020-01-01 Epub Date: 2020-03-14 DOI: 10.23937/2469-570x/1410066

Mujib Ullah, Asma Akbar

DNA encodes RNA and is responsible for protein production in cells. RNA editing is the process by which genetic information is altered in the RNA molecule. RNA editing in cancer initiation, progression and development has been well documented and play an important role in tumorigenesis. Studying RNA editing and its application to change genetic information after transcription, RNA-editing technology could be an important innovation in cancer and has the potential for more effective precision treatment. Bioengineering integration approach and artificial intelligence could revolutionize the entire field of RNA editing for early detection of cancer.

DNA编码RNA，负责细胞内蛋白质的产生。RNA编辑是指在RNA分子中改变遗传信息的过程。RNA编辑在癌症的发生、进展和发展中已被充分记录，并在肿瘤发生中发挥重要作用。研究RNA编辑及其在转录后改变遗传信息方面的应用，RNA编辑技术可能是癌症领域的一项重要创新，具有更有效的精准治疗潜力。生物工程整合方法和人工智能可以彻底改变整个RNA编辑领域，用于癌症的早期检测。

引用次数: 14

Mesenchymal Stem Cell Based Therapy for Parkinson's Disease 基于间充质干细胞的帕金森病治疗

International journal of stem cell research and therapy

Pub Date : 2019-09-23 DOI: 10.23937/2469-570x/1410062

A. SalemNeveen

Parkinson’s disease (PD) is a chronic, progressive, neurodegenerative disease with a multifactorial etiology, the predominant pathology of PD is the loss of dopaminergic cells in the substantia nigra. It is characterized by hallmark signs of bradykinesia, rigidity, tremor, and postural instability. Medical and pharmacological treatments for Parkinson’s disease are limited to the symptomatic relief of patients, and has failed to prevent or slow down the process of neurodegeneration. Cell transplantation is a strategy with great potential for the treatment of Parkinson’s disease, Mesenchymal stem cells are a great therapeutic cell source because they are easy accessible. They have trophic effects for protecting damaged tissues as well as differentiation ability to generate a broad spectrum of cells, including dopamine neurons, which contribute to the replenishment of lost cells in Parkinson’s disease.

帕金森病（PD）是一种慢性、进行性、神经退行性疾病，具有多因素病因，PD的主要病理学是黑质多巴胺能细胞的丧失。其特征是运动迟缓、僵硬、震颤和姿势不稳定的标志性体征。帕金森病的医学和药理学治疗仅限于患者的症状缓解，未能预防或减缓神经退行性变的过程。细胞移植是治疗帕金森病的一种极具潜力的策略，间充质干细胞是一种很好的治疗细胞来源，因为它们很容易获得。它们具有保护受损组织的营养作用，以及产生广泛细胞的分化能力，包括多巴胺神经元，这有助于补充帕金森病中丢失的细胞。

引用次数: 3

Potential of Polycaprolactone Nanofiber Scaffold for Ex Vivo Expansion of Cord Blood-Derived CD34+ Hematopoietic Stem Cells 聚己内酯纳米纤维支架体外扩增脐血CD34+造血干细胞的潜力

International journal of stem cell research and therapy

Pub Date : 2019-06-30 DOI: 10.23937/2469-570X/1410059

Mousavi Seyed Mohammad Hadi, A. Saeid, S. Masoud, M. Javad

Background: An efficient and practical ex vivo expansion of cord blood hematopoietic stem cells as an alternative source of HSC transplantation is crucial in understanding the potential of HSC transplantation in treating or supportive therapy in a variety of hematologic and non-hematologic disorders. The aim of this study was an ex vivo expansion of cord blood hematopoietic stem cells in a novel threedimensional polycaprolactone nanofiber scaffold coated with collagen. Methods: After 10-day cultured of cord blood CD34+ cells in 2-Dimensional and 3-Dimensional culture system, the evaluation was performed by qRT-PCR, flow cytometry and clonogenicity. Results: 3-Dimensional Polycaprolactone nano-scaffold coated with collagen provided higher total nucleated cells (50-fold vs. 38-fold) and CD34+ cells (20-fold vs. 2.6-fold) (p < 0.05) and compared to 2-Dimensional cell culture and before expansion had higher expression of homing and self-renewal genes and for VLA-4, hTERT and BMI-1 genes were statically significant (p = 0.0001). The expression of myeloid markers in 3-Dimensional scaffold was significantly higher than the 2-Dimensional culture system (p < 0.05). The total colony in 2-Dimensional culture was lower than 3-Dimensional culture medium (p < 0.05). Conclusion: This study demonstrated the synergistic effect between the three-dimensionality of the scaffold and collagen as an extracellular matrix protein, and the potential of this 3-Dimensional Polycaprolactone nanofiber scaffold coated with collagen for ex vivo expansion of HSCs.

背景:高效、实用的脐带血造血干细胞体外扩增作为造血干细胞移植的替代来源，对于理解造血干细胞移植在多种血液学和非血液学疾病的治疗或支持治疗中的潜力至关重要。这项研究的目的是脐带血造血干细胞体外扩增在一个新的三维聚己内酯纳米纤维支架涂有胶原蛋白。方法:将脐带血CD34+细胞分别在二维和三维培养体系中培养10 d后，采用qRT-PCR、流式细胞术和克隆原性等方法进行评价。结果:胶原包被的三维聚己内酯纳米支架提供了更高的总有核细胞(50倍比38倍)和CD34+细胞(20倍比2.6倍)(p < 0.05)，与二维细胞培养和扩增前相比，归巢和自我更新基因的表达更高，VLA-4、hTERT和BMI-1基因的表达有统计学意义(p = 0.0001)。骨髓标志物在三维支架中的表达显著高于二维培养体系(p < 0.05)。二维培养基中菌落总数低于三维培养基(p < 0.05)。结论:本研究证明了支架的三维性与作为细胞外基质蛋白的胶原之间的协同作用，以及胶原包被的三维聚己内酯纳米纤维支架体外扩增造血干细胞的潜力。

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引用次数: 4

Testicular Expression of SCP-3, Gfra, and Sca-1 in Induced Azoospermatic Male Mice Treated with Honey, Bovine Colostrum and Umbilical Cord Blood Derived Mesenchymal Stem Cells 蜂蜜、牛初乳和脐带血间充质干细胞诱导的无精子雄性小鼠睾丸中SCP-3、Gfra和Sca-1的表达

International journal of stem cell research and therapy

Pub Date : 2019-06-30 DOI: 10.23937/2469-570X/1410060

Hassan Somia, Z. Faten, W. Shimaa

引用次数: 0

Human Umbilical Cord Stem Cells in Chitosan Attenuate Myocardial Injury in Rat Cardiac Infarction 壳聚糖人脐带干细胞对心肌梗死大鼠心肌损伤的影响

International journal of stem cell research and therapy

Pub Date : 2019-06-24 DOI: 10.23937/2469-570X/1410061

R. Henning, Abraham Khan, Xiao Wang

Background: Myocardial infarction (MI) is the leading cause of cardiovascular deaths and disability in the industrialized world. Although stem cells have been injected into hearts to limit MI damage, < 4% of stem cells remain in the heart for > 1 hour due to myocardial contractility which causes the rapid egress of the stem cells through the cardiac veins and lymphatics. We hypothesized that stem cells in chitosan gels would remain longer in the heart and therefore be more beneficial in MI repair. Methods: 100 rats with MIs were divided into Control, Chitosan Gel, human umbilical cord stem cells (hUCBC), or hUCBC in Gel groups for MI injections. Echocardiograms were done before and at 2, 4, and 8 weeks after injections then random hearts were examined for infarct size and histopathology at each time. Results: Control infarcts averaged 25 ± 1%, 26.5 ± 1% and 27 ± 1% of the ventricular area at 2, 4, and 8 weeks. hUCBC in Gel infarcts were smaller than Control, Gel or hUCBC infarcts and averaged 13 ± 0.9%, 11 ± 0.9% and 11 + 0.9% (P < 0.001 vs. controls). hUCBC in Gel periinfarct thicknesses averaged 889 ± 10 μm at 4 weeks and were greater than other groups (P < 0.05). Arteriole densities in hUCBC in Gels averaged 6.3 ± 0.2/high power field and were greater than other Groups (P < 0.05). LV fractional shortening (FS) averaged 49 ± 1% prior to MI and decreased in Controls to 24 ± 1.1%, 16.8 ± 1.2%, and 19.9 ± 1.1%. hUCBC in Gel FS were greater than other groups and averaged 38 ± 1.0%, 38 ± 1.0%, and 39 ± 1.0 (P < 0.001 vs. controls). hUCBC in Gel LV diastolic diameters were smaller than Gel or hUCBC Groups, averaged 0.68 ± 0.05, 0.65 ± 0.03, and 0.64 ± 0.04 (P < 0.05 vs. controls), and were similar to normals. Conclusion: hUCBC in Chitosan Gel exceed Chitosan or hUCBC in decreasing MI size and LV remodeling and increasing peri-infarct ventricular thickness and neovascularization.

背景：心肌梗死（MI）是工业化国家心血管死亡和致残的主要原因。尽管干细胞已被注射到心脏中以限制MI损伤，但由于心肌收缩性，<4%的干细胞在心脏中停留>1小时，这导致干细胞通过心静脉和淋巴管快速排出。我们假设壳聚糖凝胶中的干细胞在心脏中停留的时间更长，因此对心肌梗死的修复更有益。方法：将100只MI大鼠分为对照组、壳聚糖凝胶组、人脐带干细胞（hUCBC）组或hUCBC凝胶组进行MI注射。在注射前和注射后2、4和8周进行超声心动图检查，然后随机检查心脏每次的梗死面积和组织病理学。结果：对照组梗死在2、4和8周时平均占心室面积的25±1%、26.5±1%和27±1%。凝胶梗死中的hUCBC小于对照组、凝胶或hUCBC梗死，平均为13±0.9%、11±0.9%和11±0.9%（与对照组相比P＜0.001）。Gel梗死周围hUCBC厚度在4周时平均为889±10μm，且大于其他组（P<0.05）。Gel中hUCBC的动脉密度平均为6.3±0.2/高功率场，且高于其他组（P＜0.05）。MI前左心室缩短分数（FS）平均为49±1%，对照组降至24±1.1%、16.8±1.2%，和19.9±1.1%。Gel FS中的hUCBC高于其他组，平均为38±1.0%、38±1.0%和39±1.0（与对照组相比P＜0.001）。Gel左心室舒张直径中的hUCBC小于Gel或hUCBC组，平均0.68±0.05、0.65±0.03和0.64±0.04（与对照组相比P<0.05），并且与正常人相似。结论：壳聚糖凝胶中的hUCBC在降低心肌梗死面积和左心室重构、增加梗死周围心室厚度和新生血管方面优于壳聚糖或hUCBC。

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引用次数: 1

Fluorescence-Activated on-Chip Cell Culture Sorting (O3CS): Smart Petri Dish 荧光激活片上细胞培养分选(O3CS):智能培养皿

International journal of stem cell research and therapy

Pub Date : 2018-12-31 DOI: 10.23937/2469-570X/1410055

G MolchanovPavel, E Moreno-CuevasJorge, Hernández-Torre Martín, Gonzalez-Garza Maria Teresa, Garcia Charles, Hillhouse Edward William, L. Christine, M ZsigmondEva, W. Rick, M. John, Zeng Hong, Kim Jeewon, Yang Jieying, Cuevas Raquel, M BulaiPavel, N PitlikTaras, A DenisovAndrey, N CherenkevichSergey, Molchanova Alla Yu, N GolubevaElena, A StrukovVictor, Boksha Victor

On-chip cell sorting is a promising technique for sorting stem cells in culture. On-chip cell sorting allows minimization of lab personnel involvement in cells processing, dramatically reducing the risk of cell culture contamination. We developed a fluorescence-activated On-Chip Cell Culture Sorting (O3CS) system, which combines a biocompatible semiconductor light addressable microarray (chip) and optical setup for chip addressing and cell culture observation. The optical setup has fluorescent and reflected-light microscope capability for visualization and control of cell populations. High-resolution detection of ‘unwanted’ cells with a high-efficient sorter, based on light-induced electroporation is in the core of the O3CS implemented in NeuroSyntek StemOptimizer 6+. We demonstrated capability of the system to perform cell culture fluorescence activated sorting by inducing irreversible single-cell electroporation, validated O3CS sorting efficacy with fluorescent microscopy and flow cytometry, and compared it with the magnetic-activated cell sorting, demonstrating vastly superior performance in selectivity, efficiency, and sorting speed. RESEARCH ARTiCLE

芯片上细胞分选是一种很有前途的干细胞培养分选技术。芯片上的细胞分选可以最大限度地减少实验室人员参与细胞处理，大大降低细胞培养污染的风险。我们开发了一种荧光激活的片上细胞培养分选(O3CS)系统，该系统结合了生物相容性半导体光寻址微阵列(芯片)和用于芯片寻址和细胞培养观察的光学装置。光学装置具有荧光和反射光显微镜功能，用于可视化和控制细胞群。基于光诱导电穿孔的高效分选器对“不需要的”细胞进行高分辨率检测是NeuroSyntek StemOptimizer 6+中实现的O3CS的核心。我们证明了该系统能够通过诱导不可逆的单细胞电穿孔来进行细胞培养荧光激活分选，并通过荧光显微镜和流式细胞术验证了O3CS分选的有效性，并将其与磁激活细胞分选进行了比较，结果表明该系统在选择性、效率和分选速度方面具有极大的优势。研究文章

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引用次数: 0

Mechanical Stimulation Increases the Proliferation and Differentiation Potential of Human Adipose-Derived Stromal Cells 机械刺激增加人脂肪来源基质细胞的增殖和分化潜能

International journal of stem cell research and therapy

Pub Date : 2018-12-31 DOI: 10.23937/2469-570X/1410056

Jungwirth Susanne, Bohner Lauren, Spindler Kathrin, Hanisch Marcel, Kleinheinz Johannes, Sielker Sonja

Background: Dynamic conditions stimulate the bone remodeling process by improving the nutrients transport and increasing the expression of osteogenic cells. The purpose of this study was to evaluate the effect of mechanical stimulation on the osteogenic differentiation of human adipose-derived stromal cells. Methods: Cells were cultured under static and dynamic conditions in collagen scaffolds for 14 days. The mechanical stimulation was performed using a biaxial rotating bioreactor (5 × rpm and perfusion flow rate of 10 × rpm). Cell viability was analyzed with a living cell count and a MTT assay. Changes in expression of specific stem cell marker, osteogenic marker and endothelial markers were analyzed on gene (RT-qPCR) and protein (IHC) level. Data were statistically analyzed by one-way ANOVA (p = 0.05). Results: Cell viability was higher under dynamic condition and cells migrated deeper in the collagen matrix. Expression of stem cell marker (ANPEP/CD13, CD44, THY1/CD90) was significant higher under dynamic condition. This was also observed for osteogenic markers (collagen 1, osteopontin, osteonectin). Conclusion: The mechanical stimulation increased significantly cell viability and differentiation potential of human adipose-derived stromal cells.

背景:动态条件通过改善营养物质运输和增加成骨细胞的表达来刺激骨重塑过程。本研究旨在探讨机械刺激对人脂肪源性基质细胞成骨分化的影响。方法:细胞在胶原支架中静、动态培养14 d。机械刺激采用双轴旋转生物反应器(5 × rpm，灌注流速为10 × rpm)。用活细胞计数和MTT法分析细胞活力。从基因(RT-qPCR)和蛋白(IHC)水平分析特异性干细胞标志物、成骨标志物和内皮标志物的表达变化。资料采用单因素方差分析(p = 0.05)。结果:动态条件下细胞活力高，细胞在胶原基质中迁移深度大。动态条件下，干细胞标志物(ANPEP/CD13、CD44、THY1/CD90)的表达显著升高。在成骨标志物(胶原蛋白1、骨桥蛋白、骨粘连蛋白)中也观察到这一点。结论:机械刺激可显著提高人脂肪源性基质细胞的细胞活力和分化潜能。

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引用次数: 2

Late Intrathecal Cell Therapy Increases Brain Glucose Metabolism and Improves the Long-Term Established Sequelae of Cerebral Hemorrhage 晚期鞘内细胞治疗增加脑葡萄糖代谢并改善脑出血的长期后遗症

International journal of stem cell research and therapy

Pub Date : 2018-12-31 DOI: 10.23937/2469-570X/1410058

Fernández-Guinea Sara, Z. Mercedes, G. Estefania, Mucientes Jorge, L. Cristina, Fernández-Mateos Cecilia, V. Jesús

Background: The possibility of an increase in brain glucose metabolism after intrathecal administrations of autologous mesenchymal stromal cells (MSCs) has been recently reported, suggesting a role in the treatment of neurological disorders. We describe here the utility of this type of cell therapy in chronic neurological sequelae due to cerebral hemorrhage. Methods: A 49-year-old female suffered from 2009 severe neurological sequelae after spontaneous intracerebral hemorrhage open to ventricle in the left frontoparietal region. Eight years later, she received 3 intrathecal administrations of 100 million autologous MSCs each three months, until a total dose of 300 million. Results: From the first administration of MSCs, the patient reported a clear and progressive improvement that is maintained one year after finishing the cell therapy. The neurological improvement was associated to a progressive increase in global brain glucose metabolism measured by 18F-fluorodeoxyglucose positron emission tomography (18F-FDG-PET). Conclusions: Late intrathecal administration of cell therapy with autologous MSCs may be a useful strategy to achieve improvement of chronic neurological sequelae due to hemorrhagic stroke.

背景：最近有报道称，鞘内注射自体间充质基质细胞（MSC）后，大脑葡萄糖代谢增加，这表明其在治疗神经系统疾病中发挥作用。我们在这里描述了这种类型的细胞治疗在脑出血引起的慢性神经后遗症中的效用。方法：一名49岁女性，2009年左额顶区脑室自发性脑出血后出现严重神经系统后遗症。八年后，她每三个月接受3次鞘内给药，每次1亿自体骨髓间充质干细胞，直到总剂量达到3亿。结果：从第一次给药骨髓间充质干细胞开始，患者报告了明显且渐进的改善，并在完成细胞治疗一年后保持。神经系统的改善与18F-氟脱氧葡萄糖正电子发射断层扫描（18F-FDG-PET）测量的全脑葡萄糖代谢的逐渐增加有关。结论：晚期鞘内给予自体骨髓间充质干细胞细胞治疗可能是改善出血性脑卒中所致慢性神经后遗症的有效策略。

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引用次数: 0

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