Pub Date : 2024-06-03DOI: 10.46439/biomedres.5.044
Shreya Banerjee, E. Prabhakaran
Elucidation of the nature of non-covalent interactions that govern the rate-limiting cis-trans isomerism at Xaa-Pro peptide bonds is fundamental to unravelling the protein folding mechanism, the stereoelectronic control elements of the structure and dynamics of the peptide bond, and the design of novel peptide isosteres. CisPro rotamers are stabilized by very few interactions compared to the transPro rotamer and are hence relatively scarcely populated. Design of novel interactions that can bias cisPro stability, with least mutations to the prolyl peptide bond, is crucial for accessing the cisPro motif in a variety of peptides. This mini review discusses the various interactions tailored for improving cisPro stability.
{"title":"Tailoring interactions for cisPro peptide bond stabilization","authors":"Shreya Banerjee, E. Prabhakaran","doi":"10.46439/biomedres.5.044","DOIUrl":"https://doi.org/10.46439/biomedres.5.044","url":null,"abstract":"Elucidation of the nature of non-covalent interactions that govern the rate-limiting cis-trans isomerism at Xaa-Pro peptide bonds is fundamental to unravelling the protein folding mechanism, the stereoelectronic control elements of the structure and dynamics of the peptide bond, and the design of novel peptide isosteres. CisPro rotamers are stabilized by very few interactions compared to the transPro rotamer and are hence relatively scarcely populated. Design of novel interactions that can bias cisPro stability, with least mutations to the prolyl peptide bond, is crucial for accessing the cisPro motif in a variety of peptides. This mini review discusses the various interactions tailored for improving cisPro stability.","PeriodicalId":73621,"journal":{"name":"Journal of biomed research","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141271332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-03DOI: 10.46439/biomedres.5.045
Haniel Fernandes
{"title":"The halftime strategies applied to elite soccer players for rehydration and maintain performance","authors":"Haniel Fernandes","doi":"10.46439/biomedres.5.045","DOIUrl":"https://doi.org/10.46439/biomedres.5.045","url":null,"abstract":"","PeriodicalId":73621,"journal":{"name":"Journal of biomed research","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141268901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-03DOI: 10.46439/biomedres.5.043
T. Addissouky, Ibrahim El Tantawy El Sayed, Majeed M. A. Ali, Mahmood Hasen shuhata Alubiady, Yuliang Wang
Background: Glomerulonephritis refers to a range of conditions involving inflammation and injury to the kidneys' glomeruli, often leading to significant morbidity if left untreated. Purpose: This review aims to examine emerging advancements in the prevention and treatment of glomerulonephritis and highlight progress in transforming the prognosis of this spectrum of diseases, while also identifying gaps requiring ongoing effort. Main body: Novel targeted immunotherapies utilizing engineered delivery platforms and biologicals like monoclonal antibodies are progressing in research pipelines, potentially offering safer, more efficacious alternatives to current standard immunosuppression. High-throughput biomarker assays and AI/machine learning algorithms have demonstrated the ability to improve early detection of kidney damage and guide personalized treatment plans. Further prevention opportunities emerge from modulating microbiome-immune interactions, lifestyle factors, and vaccinations shielding against infections triggering renal disorders. Conclusion: Although challenges remain, recent advancements in unraveling the pathogenesis of glomerulonephritis coupled with the emergence of cutting-edge diagnostics and targeted interventions set the stage for a new era combating the risk and progression of this spectrum of diseases.
{"title":"Transforming glomerulonephritis care through emerging diagnostics and therapeutics","authors":"T. Addissouky, Ibrahim El Tantawy El Sayed, Majeed M. A. Ali, Mahmood Hasen shuhata Alubiady, Yuliang Wang","doi":"10.46439/biomedres.5.043","DOIUrl":"https://doi.org/10.46439/biomedres.5.043","url":null,"abstract":"Background: Glomerulonephritis refers to a range of conditions involving inflammation and injury to the kidneys' glomeruli, often leading to significant morbidity if left untreated.\u0000\u0000Purpose: This review aims to examine emerging advancements in the prevention and treatment of glomerulonephritis and highlight progress in transforming the prognosis of this spectrum of diseases, while also identifying gaps requiring ongoing effort.\u0000\u0000Main body: Novel targeted immunotherapies utilizing engineered delivery platforms and biologicals like monoclonal antibodies are progressing in research pipelines, potentially offering safer, more efficacious alternatives to current standard immunosuppression. High-throughput biomarker assays and AI/machine learning algorithms have demonstrated the ability to improve early detection of kidney damage and guide personalized treatment plans. Further prevention opportunities emerge from modulating microbiome-immune interactions, lifestyle factors, and vaccinations shielding against infections triggering renal disorders.\u0000\u0000Conclusion: Although challenges remain, recent advancements in unraveling the pathogenesis of glomerulonephritis coupled with the emergence of cutting-edge diagnostics and targeted interventions set the stage for a new era combating the risk and progression of this spectrum of diseases.","PeriodicalId":73621,"journal":{"name":"Journal of biomed research","volume":"22 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141270887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-21DOI: 10.46439/biomedres.5.037
Matthew Goldman
Anki is an open-sourced educational platform that utilizes a spaced repetition-based algorithm for information retention. This application has become increasingly popular among medical students and residents over recent years. The aim of this review was to describe this novel education technique, provide reasons for its adoption, and summarize the available literature regarding the efficacy of this method. With the recent COVID-19 pandemic, the medical education paradigm has undergone dynamic shifts. With self-learning independent of lectures on the rise prior to the pandemic, COVID-19 served as a catalyst to increase Anki popularity. The all-encompassing, free of charge, and open-sourced structure of Anki was an ideal fit during times of isolated study. This review will allow current residents and medical students to recognize advantages of this evidence-proven study techniques to increase learning efficacy. A systemic review of papers across all medical journals available on PubMed was conducted to identify studies published without time constraints. The search was for (Anki) and (spaced repetition residency). Available outcome data was summarized and discussed for residents and medical students independently. We found that Anki use has steadily increased among both medical students and residents. The application was regarded highly by users when surveyed. Many studies showed a statistically significant increase in exam performance associated with Anki use and high levels of satisfaction among residents and medical students. As burnout and time constrictions threaten educational workflow, Anki may serve as a powerful tool to improve the quality of learning. Further data needs to be collected and analyzed in residency specialties where Anki use may already exist but be underreported. Additionally, research is warranted to provide more high-quality evidence of Anki’s efficacy among medical students. Further research can be used to promote adoption of this technique among medical students and pre-meds early in their careers.
Anki 是一个开源的教育平台，利用基于间隔重复的算法来保持信息。近年来，该应用程序在医学生和住院医师中越来越受欢迎。本综述旨在描述这种新颖的教育技术，提供其被采用的原因，并总结有关该方法有效性的现有文献。随着最近 COVID-19 的流行，医学教育模式发生了动态变化。在 COVID-19 大流行之前，独立于讲课的自学方式正在兴起，而 COVID-19 则成为 Anki 更受欢迎的催化剂。Anki 的结构包罗万象、免费且开源，是孤立学习时期的理想选择。本综述将使目前的住院医师和医科学生认识到这一经过实证验证的学习技巧在提高学习效率方面的优势。我们对PubMed上所有医学期刊上的论文进行了系统性审查，以确定在没有时间限制的情况下发表的研究。搜索对象为（Anki）和（间隔重复住院医师培训）。对住院医师和医学生的现有结果数据进行了总结和独立讨论。我们发现，Anki 在医学生和住院医师中的使用率稳步上升。在接受调查时，用户对该应用程序给予了高度评价。许多研究表明，Anki 的使用在统计学上显著提高了考试成绩，而且住院医师和医学生的满意度也很高。由于职业倦怠和时间限制对教育工作流程构成威胁，Anki 可能成为提高学习质量的有力工具。需要进一步收集和分析住院医师专科的数据，因为这些专科可能已经使用了 Anki，但却没有充分报告。此外，还需要开展研究，提供更多高质量的证据来证明 Anki 在医学生中的功效。进一步的研究可用于促进医学生和医学预科生在职业生涯早期采用这种技术。
{"title":"Evidence-based educational algorithm “Anki” for optimization of medical education","authors":"Matthew Goldman","doi":"10.46439/biomedres.5.037","DOIUrl":"https://doi.org/10.46439/biomedres.5.037","url":null,"abstract":"Anki is an open-sourced educational platform that utilizes a spaced repetition-based algorithm for information retention. This application has become increasingly popular among medical students and residents over recent years. The aim of this review was to describe this novel education technique, provide reasons for its adoption, and summarize the available literature regarding the efficacy of this method. With the recent COVID-19 pandemic, the medical education paradigm has undergone dynamic shifts. With self-learning independent of lectures on the rise prior to the pandemic, COVID-19 served as a catalyst to increase Anki popularity. The all-encompassing, free of charge, and open-sourced structure of Anki was an ideal fit during times of isolated study. This review will allow current residents and medical students to recognize advantages of this evidence-proven study techniques to increase learning efficacy. A systemic review of papers across all medical journals available on PubMed was conducted to identify studies published without time constraints. The search was for (Anki) and (spaced repetition residency). Available outcome data was summarized and discussed for residents and medical students independently. We found that Anki use has steadily increased among both medical students and residents. The application was regarded highly by users when surveyed. Many studies showed a statistically significant increase in exam performance associated with Anki use and high levels of satisfaction among residents and medical students. As burnout and time constrictions threaten educational workflow, Anki may serve as a powerful tool to improve the quality of learning. Further data needs to be collected and analyzed in residency specialties where Anki use may already exist but be underreported. Additionally, research is warranted to provide more high-quality evidence of Anki’s efficacy among medical students. Further research can be used to promote adoption of this technique among medical students and pre-meds early in their careers.","PeriodicalId":73621,"journal":{"name":"Journal of biomed research","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140444109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-23DOI: 10.46439/biomedres.4.037
S. L. Choubisa
{"title":"Is drinking groundwater in India safe for human health in terms of fluoride?","authors":"S. L. Choubisa","doi":"10.46439/biomedres.4.037","DOIUrl":"https://doi.org/10.46439/biomedres.4.037","url":null,"abstract":"","PeriodicalId":73621,"journal":{"name":"Journal of biomed research","volume":"112 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80681677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-23DOI: 10.46439/biomedres.4.036
S. Bittmann, E. Luchter, Lara Bittmann, E. Moschüring-Alieva, G. Villalon
{"title":"Pulmonary agenesis in a newborn","authors":"S. Bittmann, E. Luchter, Lara Bittmann, E. Moschüring-Alieva, G. Villalon","doi":"10.46439/biomedres.4.036","DOIUrl":"https://doi.org/10.46439/biomedres.4.036","url":null,"abstract":"","PeriodicalId":73621,"journal":{"name":"Journal of biomed research","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77783797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.46439/biomedres.4.035
Andrey E. Urusov, Kseniya S. Aulova, Pavel S. Dmitrenok, Valentina N. Buneva, Georgy A. Nevinsky
Introduction: Histones play crucial roles in chromatin functioning and gene transcription, but they are incredibly harmful in intercellular space since they stimulate systemic inflammatory and toxic responses.Myelin basic protein (MBP) is the most meaningful protein of the axon myelin-proteolipid sheath.Antibodies with different enzymatic activities (abzymes) are substantial and specific features of some autoimmune diseases. Methods: IgG preparations against individual histones (H1, H2A, H2B, H3, and H4) and MBP were obtained from the blood plasma of experimental autoimmune encephalomyelitis-prone C57BL/6 mice by several affinity chromatographies. These antibodies corresponded to different stages of experimental autoimmune encephalomyelitis (EAE) development: spontaneous, MOG (myelin oligodendrocyte glycoprotein), and DNA-histones complex accelerated onset, acute, and remission stages. Results: IgG-abzymes against MBP and five individual histones demonstrated unusual complex formation polyreactivity and enzymatic cross-reactivity in the specific hydrolysis of histone H1. All IgGs of 3-month-old mice (zero time) against MBP and individual histones demonstrated from 4 to 23 different H1 hydrolysis sites. The spontaneous achievement of EAE during 60 days led to a powerful change in the type and number of H1 hydrolysis sites by IgGs against five histones and MBP. Treatment of mice with MOG and DNA-histones complex results in alteration in IgGs activities, a change in type, and an increase or decrease in the number of H1 hydrolysis sites compared to zero time. The minimum number (4) of different H1 hydrolysis sites was found for IgGs against Н2B (zero time) and Н2A (MOG treatment), while the maximum (26) – against H1 (MOG treatment). Conclusion: Generally, it first demonstrated that at different stages of EAE evolution, IgG-abzymes against individual histones and MBP could significantly differ in specific sites and their number of H1 hydrolysis. Possible reasons for the catalytic cross-reactivity and strong differences in the number and type of cleavage sites are discussed.
{"title":"Experimental autoimmune encephalomyelitis of mice: Catalytic cross site-specific hydrolysis of H1 histone by IgG antibodies against H1, H2A, H2B, H3, H4 histones, and myelin basic protein","authors":"Andrey E. Urusov, Kseniya S. Aulova, Pavel S. Dmitrenok, Valentina N. Buneva, Georgy A. Nevinsky","doi":"10.46439/biomedres.4.035","DOIUrl":"https://doi.org/10.46439/biomedres.4.035","url":null,"abstract":"Introduction: Histones play crucial roles in chromatin functioning and gene transcription, but they are incredibly harmful in intercellular space since they stimulate systemic inflammatory and toxic responses.Myelin basic protein (MBP) is the most meaningful protein of the axon myelin-proteolipid sheath.Antibodies with different enzymatic activities (abzymes) are substantial and specific features of some autoimmune diseases. Methods: IgG preparations against individual histones (H1, H2A, H2B, H3, and H4) and MBP were obtained from the blood plasma of experimental autoimmune encephalomyelitis-prone C57BL/6 mice by several affinity chromatographies. These antibodies corresponded to different stages of experimental autoimmune encephalomyelitis (EAE) development: spontaneous, MOG (myelin oligodendrocyte glycoprotein), and DNA-histones complex accelerated onset, acute, and remission stages. Results: IgG-abzymes against MBP and five individual histones demonstrated unusual complex formation polyreactivity and enzymatic cross-reactivity in the specific hydrolysis of histone H1. All IgGs of 3-month-old mice (zero time) against MBP and individual histones demonstrated from 4 to 23 different H1 hydrolysis sites. The spontaneous achievement of EAE during 60 days led to a powerful change in the type and number of H1 hydrolysis sites by IgGs against five histones and MBP. Treatment of mice with MOG and DNA-histones complex results in alteration in IgGs activities, a change in type, and an increase or decrease in the number of H1 hydrolysis sites compared to zero time. The minimum number (4) of different H1 hydrolysis sites was found for IgGs against Н2B (zero time) and Н2A (MOG treatment), while the maximum (26) – against H1 (MOG treatment). Conclusion: Generally, it first demonstrated that at different stages of EAE evolution, IgG-abzymes against individual histones and MBP could significantly differ in specific sites and their number of H1 hydrolysis. Possible reasons for the catalytic cross-reactivity and strong differences in the number and type of cleavage sites are discussed.","PeriodicalId":73621,"journal":{"name":"Journal of biomed research","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136300388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-05DOI: 10.46439/biomedres.4.030
A. Kleebayoon, V. Wiwanitkit
The most significant global public health issue is still COVID-19. Due to the advent of a new variation and the widespread use of COVID-19 immunization, the disease is currently not as severe as it was in 2020. The immunization is still required, though. Here, the authors examine and offer suggestions for COVID-19 immunization as the illness transitions from an epidemic to an endemic condition.
{"title":"COVID-19 vaccination, disease severity and practice when COVID turns from endemic to epidemic disease","authors":"A. Kleebayoon, V. Wiwanitkit","doi":"10.46439/biomedres.4.030","DOIUrl":"https://doi.org/10.46439/biomedres.4.030","url":null,"abstract":"The most significant global public health issue is still COVID-19. Due to the advent of a new variation and the widespread use of COVID-19 immunization, the disease is currently not as severe as it was in 2020. The immunization is still required, though. Here, the authors examine and offer suggestions for COVID-19 immunization as the illness transitions from an epidemic to an endemic condition.","PeriodicalId":73621,"journal":{"name":"Journal of biomed research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90045305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-05DOI: 10.46439/biomedres.4.031
H. Fernandes
{"title":"On a match day, what should an elite soccer players routine look like?","authors":"H. Fernandes","doi":"10.46439/biomedres.4.031","DOIUrl":"https://doi.org/10.46439/biomedres.4.031","url":null,"abstract":"","PeriodicalId":73621,"journal":{"name":"Journal of biomed research","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72974129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-05DOI: 10.46439/biomedres.4.029
H. Fernandes
{"title":"The high-performance youth soccer players carbohydrate ingestion does not seem to change between base categories","authors":"H. Fernandes","doi":"10.46439/biomedres.4.029","DOIUrl":"https://doi.org/10.46439/biomedres.4.029","url":null,"abstract":"","PeriodicalId":73621,"journal":{"name":"Journal of biomed research","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91266402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：