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distribution of brown marmorated stink bug (Halyomorpha halys (Stål, 1855); Hemiptera: Pentatomidae) in the Northeast part of the Carpathian Lowland (West Ukraine) 褐纹臭虫的分布(Halyomorpha halys, 1855);半翅目:蝽科)喀尔巴阡低地东北部(西乌克兰)
Pub Date : 2022-05-26 DOI: 10.34101/actaagrar/1/10831
Szabolcs Szanyi, Aletta Ősz, Kálmán Szanyi, L. Potish, A. Nagy
Halyomorpha halys (Stål, 1855) spread rapidly during the last five years and became common and abundant in Central Europe, especially in the urban environment. In the Carpathian basin, the West Ukrainian Transcarpathia county was the last region where this species has not been found yet. The first specimens of this invasive pest were sampled in Velyka Dobron’ in 2018 and in 2019 it was found in another neighbouring village and cities of Chop, Berehove and Uzhhorod. The data provided here is the first for West Ukraine and the second for the country following the published record from Odessa (SE Ukraine). Although H. halys is still less abundant and occupied mainly urban habitats, it will certainly cause nuisance for peoples and damages for farmers in the near future.
Halyomorpha halys (stamatl, 1855)在过去五年中迅速传播,在中欧,特别是在城市环境中变得普遍和丰富。在喀尔巴阡盆地,西乌克兰外喀尔巴阡县是最后一个尚未发现这种物种的地区。这种入侵性害虫的第一批样本于2018年在Velyka Dobron取样,2019年在邻近的另一个村庄和城市Chop、Berehove和Uzhhorod发现了这种害虫。这里提供的数据是西乌克兰的第一个数据,是继敖德萨(乌克兰东南部)公布的记录之后该国的第二个数据。虽然halys的数量仍然较少,并且主要占据城市栖息地,但在不久的将来,它肯定会给人们带来滋扰,给农民造成损害。
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引用次数: 1
Use of molecular marker methods in the classification of bamboo taxa: A review 分子标记方法在竹类群分类中的应用综述
Pub Date : 2022-05-26 DOI: 10.34101/actaagrar/1/10392
Chan Nyein Khin, A. Veres, A. Neményi
Bamboo plants are currently attractive to researchers because of their versatile uses. Understanding the bamboos’ genetic level is needed to develop new varieties. Taxonomic identification is the basis for plant development. Bamboos were identified as their taxonomical morphological characters which are dependent on environmental factors. Molecular Marker techniques can be used to perform accurate genotype identification, which can be used for genetic diversity analyses. The RFLP, RAPD, AFLP, SSR, ISSR, iPBS, SCARS, SCoT, SRAP marker systems have been shown to be able to efficiently determine the genetic diversity of bamboo species based on genotyping. This paper summarizes research that aims to analyze the genetic diversity of bamboo species on a molecular basis.
竹子植物由于其用途广泛,目前对研究人员很有吸引力。了解竹子的遗传水平是开发新品种的必要条件。分类鉴定是植物发育的基础。竹子的分类形态特征受环境因子的影响。分子标记技术可用于进行准确的基因型鉴定,可用于遗传多样性分析。RFLP、RAPD、AFLP、SSR、ISSR、iPBS、scar、SCoT、SRAP等标记系统均能有效地确定竹种遗传多样性。本文综述了从分子角度分析竹种遗传多样性的研究进展。
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引用次数: 2
Investigation of the mycelial compatibility of Macrophomina phaseolina in the Carpatian Basin 喀尔喀特盆地菜绿巨蝇菌丝相容性的研究
Pub Date : 2022-05-26 DOI: 10.34101/actaagrar/1/10654
Kitti Csüllög, Stella Prokopics, G. Tarcali
Macrophomina phaseolina is a globally widespread fungal pathogen. The fungus has a very wide range of hosts. Under optimal conditions, M. phaseolina can cause serious damage tothe host plants. In this study, the mycelial compatibility of different M. phaseolina isolates was investigated. From 2019 to 2021, 12 sunflower samples were collected from different regions of the Carpathian Basin, 9 samples from Hungary, 1 sample from Austria, and 2 samples from Slovakia. The genetic variability of the pathogen is a critical problem in plant protection. Two compatible pathogen strains can easily exchange their genetic material for each other, which can lead to the development of resistance. All collected samples (12) were tested to examine their compatibility. Isolates from all tested samples were paired with isolates from all other samples thus, a total of 66 pairings were made. During the examinations of mycelial compatibility, only 20 pairs of all possible pairings were found to be incompatible, and all others (46) were found to be compatible.
phaseolina是一种全球广泛传播的真菌病原体。这种真菌的寄主范围很广。在最适宜的条件下,菜绿芽孢杆菌会对寄主植物造成严重的危害。本研究对不同分离菌株的菌丝相容性进行了研究。2019 - 2021年,在喀尔巴阡盆地不同地区采集了12份向日葵样本,匈牙利9份,奥地利1份,斯洛伐克2份。病原菌的遗传变异是植物保护中的一个重要问题。两个相容的病原体菌株可以很容易地交换彼此的遗传物质,这可能导致耐药性的发展。所有收集的样品(12个)都进行了相容性测试。所有测试样本的分离株与所有其他样本的分离株配对,因此,总共进行了66对配对。在菌丝相容性检查中,所有可能的配对中只有20对发现不相容,其余46对发现相容。
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引用次数: 0
Genetic structure of the Lipizzan horse breed in Hungary through the mare families 匈牙利利比赞马品种的遗传结构
Pub Date : 2022-05-26 DOI: 10.34101/actaagrar/1/10430
M. Kovács, S. Mihók
Modern animal husbandry has drastically changed the genetic structure of some domestic species. The varieties, genotypes that we think we do not need at the moment can only be saved from extinction with the help of gene conservation. Traditional Hungarian horse breeds have a long history (more than 200 years) and a demonstrably different genetic structure from other horse breeds in Europe and the rest of the world. Consequently, their enormous genetic value is undoubted. The subject of our research was to study the structure of the mare families found in the population of the Lipizzan horse breed and the genetic structure of the Hungarian population. Out of the total 61 mare families in the world, 35 are present in Hungary. There are 11 Hungarian, 12 families of Fogaras, 7 original, 4 Croatian and 1 Slovenian mare families in the Hungarian population. The proportion of mare families in Hungary and Fogaras is almost the same. The proportion of Croatian and Slovenian families is negligible. In terms of their number, they are not significant in Hungary. For this reason they have little effect on the Hungarian Lipizzan population. Except one of the original mare families are in the same situation as the Croatian and Slovenian families. The Presciana / Bradamanta mare family is the most populous of all families due to their long stay in Hungary. The proportion of families is unbalanced. Seven mare families accounting for 56.36% of the total population. Given the basic requirements of gene conservation work, this condition is far from optimal.
现代畜牧业极大地改变了一些家养物种的遗传结构。我们认为目前不需要的品种、基因型只有在基因保护的帮助下才能免于灭绝。传统的匈牙利马品种有着悠久的历史(200多年),与欧洲和世界其他地区的其他马品种有着明显不同的遗传结构。因此,它们巨大的遗传价值是毋庸置疑的。我们的研究主题是研究在利比赞马种群中发现的母马家族结构和匈牙利种群的遗传结构。在世界上总共61个马家庭中,有35个在匈牙利。匈牙利人口中有11个匈牙利马,12个福加拉马家族,7个原马家族,4个克罗地亚马家族和1个斯洛文尼亚马家族。匈牙利和福加拉斯的母马家庭比例几乎相同。克罗地亚和斯洛文尼亚家庭的比例可以忽略不计。就数量而言,他们在匈牙利并不重要。由于这个原因,它们对匈牙利利皮赞人的影响很小。除了一个原始的马家族与克罗地亚和斯洛文尼亚家族处于相同的情况。Presciana / Bradamanta mare家族是所有家族中人口最多的,因为他们在匈牙利呆了很长时间。家庭的比例不平衡。7个母马家族占总人口的56.36%。鉴于基因保护工作的基本要求,这一条件远非最佳。
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引用次数: 0
Screening of paprika (Capsicum annuum L.) varieties resistant to NaCl salt stress 抗NaCl盐胁迫的辣椒品种筛选
Pub Date : 2022-05-26 DOI: 10.34101/actaagrar/1/10420
M. Massimi, L. Radócz
Salinity stress is one of the environmental factors that negatively affect the growth and production of pepper plants. The 100 seeds' weight was measured. The total fresh weight of five seedlings and the growth rate of one seedling of three paprika varieties were also measured under the influence of NaCl at a salinity level threshold of (3 dS m‑1). The proportion of tissue water content in three pepper cultivars was measured according to a mathematical formula at the end of the experiment. In terms of seed weight, the (Carma) cultivar outperformed other types greatly. Except for the superiority of both (Carma, and Bobita F1) over (Fokusz) variety in total fresh seedlings weight under sodium chloride as abiotic stress, there are no significant differences in the total seedlings' dry weight and the rate of seedling growth. The non-drought-resistant type (Bobita F1) loses water the fastest, at 89.61%, compared to drought-tolerant kinds, which lose water at a slower rate (Carma, and Fokusz). The results demonstrate the (Carma) variety's numerical vigor, particularly in the growth rate. More testing is needed to determine the selection of varieties that are resistant to abiotic and biotic stresses.
盐胁迫是影响辣椒生长和生产的环境因子之一。测定100粒种子的重量。在盐度阈值为(3 dS m‑1)的NaCl条件下,测定了3个辣椒品种5个幼苗的总鲜重和1个幼苗的生长率。在试验结束时,根据数学公式测定了3个辣椒品种的组织含水量比例。在种子重量方面,(Carma)品种明显优于其他品种。在氯化钠非生物胁迫下,除了Carma和Bobita F1在总鲜重上优于Fokusz品种外,在总干重和幼苗生长速率上无显著差异。非抗旱性品种(Bobita F1)失水最快,为89.61%,而抗旱性品种(Carma和Fokusz)失水速度较慢。结果表明,(Carma)品种具有较强的数值活力,特别是在生长速率方面。需要更多的试验来确定对非生物和生物胁迫具有抗性的品种的选择。
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引用次数: 1
roles of mycotoxins in cereal crops production: A comparative study of Hungary and Tanzania 真菌毒素在谷类作物生产中的作用:匈牙利和坦桑尼亚的比较研究
Pub Date : 2022-05-26 DOI: 10.34101/actaagrar/1/10833
Muhoja Sylivester Nyandi, P. Pepó
Although Hungary and Tanzania's climatic, soil, and technological conditions differ significantly in crop production, cereals crop cultivation is of paramount importance; maize crop dominates the cultivated area (Hungary 1 million ha, Tanzania 3 million ha) both from a feed and food point of view. Unfortunately, in both countries, fungal species (Fusaria, Aspergilli, Penicillia, etc.) that produce various mycotoxins on cereals, including maize grains, are a growing concern. The situation is complicated because these fungal species and their toxins can appear not only on cereals but also on other crops. Despite the prevalence of mycotoxins in both countries, studies show higher exposure risks and contamination above tolerable levels for human consumption in Tanzania to Hungary, with Tanzania observing acute aflatoxicosis.
尽管匈牙利和坦桑尼亚的气候、土壤和技术条件在作物生产方面存在显著差异,但谷物作物种植至关重要;从饲料和食物的角度来看,玉米作物在耕地面积上占主导地位(匈牙利100万公顷,坦桑尼亚300万公顷)。不幸的是,在这两个国家,对谷物(包括玉米谷物)产生各种真菌毒素的真菌物种(镰刀菌、曲霉菌、青霉等)日益受到关注。情况很复杂,因为这些真菌种类及其毒素不仅会出现在谷物上,还会出现在其他作物上。尽管真菌毒素在这两个国家普遍存在,但研究表明,从坦桑尼亚到匈牙利,接触真菌毒素的风险更高,污染程度超过了人类消费的可容忍水平,坦桑尼亚观察到急性黄曲霉中毒。
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引用次数: 0
Early evaluation of use of fermented chicken manure products in practice of apple nutrient management 发酵鸡粪产品在苹果营养管理实践中的应用初步评价
Pub Date : 2022-05-26 DOI: 10.34101/actaagrar/1/8502
F. Tóth, J. Tamás, P. Nagy
According to the Green Deal efforts, the importance and relevance of organic fertilization will increase in the near future. Therefore, the investigation of the effects of different organic fertilizers on soil productivity and nutrient supply is a priority area of agricultural research. Organic fertilizer experiment was conducted in an eight-year-old apple (Malus domestica Borkh.) orchard at Debrecen-Pallag. In the trial Pinova cultivar was used. In this study, two different fermented chicken manure products were added to the soil (in 20 cm depth) to test their effects on soil nutrient status, plant uptake and fruit quality. It was found that the applied treatments slightly increased the pH and nutrient levels in almost all cases, but significant effect was not observed in all treatments compared to the control. Leaf nutrient contents (N, P, K, Ca and Mg) were measured in the experiment. Leaf nutrient status was not affected by the fermented chicken manure treatments. However, used treatments had strong effects on the fruit characteristics and inner parameters, such as fruit diameter and Brix value. Moreover, it was established that the applied organic fertilizers increased the yield significantly.
根据绿色协议的努力,在不久的将来,有机施肥的重要性和相关性将会增加。因此,研究不同有机肥对土壤生产力和养分供应的影响是农业研究的重点领域。在德布勒森-帕拉格一个8年生苹果果园进行了有机肥试验。试验选用Pinova品种。本试验通过在土壤中(深度为20 cm)添加两种不同的发酵鸡粪制品,研究其对土壤养分状况、植物吸收和果实品质的影响。结果表明,施施处理在几乎所有情况下均能使土壤pH值和养分水平略有提高,但与对照相比,并非所有处理均有显著提高。试验测定了叶片养分含量(N、P、K、Ca、Mg)。发酵鸡粪处理对叶片营养状况无显著影响。不同处理对果实性状和果实直径、糖度值等内部参数影响较大。结果表明,施用有机肥可显著提高产量。
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引用次数: 2
effect of β-glucan, carotenoids, oligosaccharides and anthocyanins on bacteria groups of excreta in broiler chickens β-葡聚糖、类胡萝卜素、低聚糖和花青素对肉鸡排泄物细菌群的影响
Pub Date : 2022-05-26 DOI: 10.34101/actaagrar/1/10639
Brigitta Csernus, S. Biró, L. Babinszky, L. Stündl, J. Remenyik, Georgina Pesti-Asbóth, J. Oláh, L. Czegledi
This study was conducted to examine the effect of natural compounds, such as β-glucan, carotenoids, oligosaccharides, and anthocyanins in the diet on bacteria gropus of excreta in Ross 308 broiler chickens. Chickens were fed 5 diets: control (basal) diet, a diet supplemented by β-glucan at 0.05%, and diets supplemented by carotenoids, oligosaccharides, or anthocyanins at 0.5% of each compound. On experimental day 19, excreta were collected to determine the proportion of Lactobacillus, Bifidobacterium, Campylobacter, Clostridium, and Escherichia coli. Samples were collected aseptically and snap-frozen in liquid nitrogen. Bacterial DNA was isolated from samples, then polymerase chain reaction using primer pairs designed to the 16S rDNA of bacterial groups were applied to define the proportion of the mentioned bacteria. Another universal primer pair was used to amplify a region of 16S rDNA of all the examined bacteria. Proportion of each bacterial groups was determined relatively to the intensity of universal PCR product band by gel documenting system and ImageLab software. Based on the results, carotenoids and anthocyanins increased the proportion of Bifidobacterium, which might imply the beneficial effects of the mentioned compounds on the bacteria composition of excreta.
本试验旨在研究饲粮中β-葡聚糖、类胡萝卜素、低聚糖和花青素等天然化合物对罗斯308肉鸡排泄物细菌群的影响。各组饲喂5种饲粮:对照(基础)饲粮、添加0.05% β-葡聚糖的饲粮和添加0.5%类胡萝卜素、低聚糖或花青素的饲粮。实验第19天,收集排泄物,测定乳酸菌、双歧杆菌、弯曲杆菌、梭状芽孢杆菌和大肠杆菌的比例。样品无菌收集,并在液氮中快速冷冻。从样品中分离细菌DNA,采用针对细菌群16S rDNA设计的引物对进行聚合酶链反应,确定细菌比例。另一个通用引物对用于扩增所有被检测细菌的16S rDNA区域。通过凝胶记录系统和ImageLab软件相对于通用PCR产物带的强度确定各细菌群的比例。结果表明,类胡萝卜素和花青素增加了双歧杆菌的比例,这可能暗示了上述化合物对排泄物细菌组成的有益作用。
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引用次数: 1
Effects of fermented chicken manure products on the N mineralization rate of the soil using the incubation method 发酵鸡粪对土壤氮矿化率的影响
Pub Date : 2022-05-26 DOI: 10.34101/actaagrar/1/10363
F. Tóth, J. Tamás, P. Nagy
In our study, the effect of fermented and specially added poultry manure products (superabsorbent polymer (SAP), bentonite and Aegis as a mycorrhizal inoculum) were investigated in a short soil incubation experiment – at 60% water capacity level - on sandy soil. Soil samples were collected from two layers of the incubation pots after the second and fourth week to check the status of the tested products and the processes in the soil. The pH and the electric conductivity (EC) of the samples were measured using an electrochemical method, while the ammonium and nitrate content of the samples was determined with a photometric method. Soil pH and EC values slightly were decreased during the experiment. Our results pointed out that the increasing dose of SAP caused lower soil pH. The nitrate content of the soil did not change significantly during the experiment. It was found that the increasing SAP content in the products, due to its cross-linked structural property, protected the nitrate ions from leaching. Our results suggest that applied SAP does not bind the nutrient ions so tightly in its structure that it competes with the plant for uptake.
本研究在沙质土壤上进行了短期土壤培养试验,研究了发酵和特殊添加的禽粪肥产品(高吸水性聚合物(SAP)、膨润土和Aegis作为菌根接种剂)在60%水量水平下对菌根的影响。在第2周和第4周后从两层培养罐中采集土壤样品,以检查被试产品的状态和土壤中的过程。用电化学法测定样品的pH值和电导率,用光度法测定样品的铵态氮和硝态氮含量。土壤pH和EC值在试验期间略有下降。结果表明,随着施SAP量的增加,土壤ph值降低,土壤硝酸盐含量在试验过程中变化不明显。发现产品中SAP含量的增加,由于其交联的结构性质,保护了硝酸盐离子的浸出。我们的研究结果表明,施用的SAP不会在其结构中紧密结合营养离子,从而与植物竞争吸收。
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引用次数: 0
efficacy of combining paraffin oil with conventional fungicide treatments against grape powdery mildew in Eger 石蜡油与常规杀菌剂联用对葡萄白粉病的防治效果
Pub Date : 2022-05-26 DOI: 10.34101/actaagrar/1/10132
X. Pálfi, Z. Karácsony, J. Kátai, Zs. Zsófi
We aimed to test the combination of paraffin oil (PFO) with regular fungicide treatment to assess its efficacy against grape powdery mildew (GPM) in a small spraying experiment on two Vitis vinifera L. cultivars (Chardonnay and Kékfrankos) with different susceptibility to Erysiphe necator. The visual symptoms of GPM on leaves and clusters were examined at three phenological states. The harvest yield was characterized by two methods, data were analyzed with one-way ANOVA and Tukey post-hoc test. Regular fungicide treatment (CT) and its combinations with PFO showed better results in both varieties to repress GPM in 2015 relative to sole PFO treatments. Mean values of combined treatments were often lower than CT but did not differ significantly from each other. The same was observed in 2016, despite the higher pressure of GPM, and missed the third survey. No significant differences were detected between treatments in yield. In contrast, the mean cluster weight of CT and combined treatments resulted in (insignificantly) higher values in each variety and year. In summary, the sole PFO showed some disease control capability as reported earlier, but this effect was greatly affected by the given vintage. Combining PFO with CT resulted in increased protection against GPM relative to the solely applied fungicides. However, this effect was not significant in all cases. It also depended on the vintage and cultivar characteristics. The beneficial impact of paraffin oil as an additive to CT may be due to the induction of plant stress responses and/or its ability to support the adherence and absorption of the combined agents.
摘要以葡萄白粉病(GPM)易感程度不同的葡萄品种霞多丽(Chardonnay)和ksamukfrankos为试验对象,对石蜡油(PFO)与常规杀菌剂联合施用进行了小型喷施试验,以评价其对葡萄白粉病(GPM)的防治效果。在三种物候状态下检测了GPM在叶片和簇上的视觉症状。采用两种方法对收获产量进行表征,数据采用单因素方差分析和Tukey事后检验。2015年,常规杀菌剂处理(CT)及其与PFO联合处理对两个品种的GPM抑制效果均优于单独使用PFO处理。联合治疗的平均值往往低于CT,但彼此间无显著差异。2016年也出现了同样的情况,尽管GPM的压力更高,但未能参加第三次调查。处理间产量无显著差异。相比之下,CT和联合处理在各品种和年份的平均聚类权值(不显著)更高。综上所述,如前所述,唯一的PFO显示出一定的疾病控制能力,但这种效果受给定年份的影响很大。与单独使用杀菌剂相比,将PFO与CT结合使用可提高对GPM的防护。然而,这种影响并非在所有情况下都显著。这也取决于年份和品种的特点。石蜡油作为CT添加剂的有利影响可能是由于其诱导植物胁迫反应和/或其支持组合剂的粘附和吸收的能力。
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引用次数: 0
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Acta Agraria Debreceniensis
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