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Commentary: "Interference of Doxycycline Pretreatment in a Model of Abdominal Aortic Aneurysms" 评论:《强力霉素预处理对腹主动脉瘤模型的干扰》
Pub Date : 2018-11-01 DOI: 10.29245/2578-3009/2018/6.1155
K. Mata
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引用次数: 1
A Review of a Diagnostic Tool: Galactomannan 半乳甘露聚糖是一种诊断工具
Pub Date : 2018-09-01 DOI: 10.29245/2578-3009/2018/5.1137
D. Karapınar
Invasive fungal infections, including invasive aspergillosis are associated with a high morbidity and mortality especially in immunocompromised patients. Diagnosis is often difficult due to several factors such as delay in clinical suspicion and the lack of spesific clinical findings. Galactomannan is a polysaccharide cell wall component of Aspergillus and galactomannan antigen detection has become widely used for diagnosis of invasive aspergillosis. Here, we tried to discuss the diagnostic value of the galactomannan test in the context of literature review. A Review of a Diagnostic Tool: Galactomannan Gulhadiye Avcu1, Deniz Yilmaz Karapinar2* 1Ege University Faculty of Medicine, Children’s Hospital, Department of Pediatric Infectious Disease, 35040 Bornova Izmir, Turkey 2Ege University Faculty of Medicine, Children’s Hospital, Pediatric Hematology, 35040 Bornova Izmir, Turkey
侵袭性真菌感染,包括侵袭性曲霉病,与高发病率和死亡率相关,特别是在免疫功能低下的患者中。诊断往往是困难的,由于几个因素,如延误临床怀疑和缺乏具体的临床表现。半乳甘露聚糖是曲霉多糖细胞壁成分,半乳甘露聚糖抗原检测已广泛应用于侵袭性曲霉病的诊断。在此,我们试图在文献回顾的背景下讨论半乳甘露聚糖试验的诊断价值。一种诊断工具的综述:半乳甘露annan Gulhadiye Avcu1, Deniz Yilmaz Karapinar2* 1埃格大学医学院,儿童医院,儿科传染病科,35040 Bornova Izmir,土耳其2埃格大学医学院,儿童医院,儿科血液学,35040 Bornova Izmir,土耳其
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引用次数: 8
Demonstration of Mosquito Midgut Antigen for the Effective Control of Mosquito Population 蚊中肠抗原对蚊虫种群控制效果的论证
Pub Date : 2018-09-01 DOI: 10.29245/2578-3009/2018/5.1148
Sabahat Abdullah, S. Rahman, A. Naveed, Pakistan
Mosquito-borne diseases can be reduced drastically with the aid of vaccines which provoke mosquitocidal or mosquito-killing effect. The midgut of mosquito performs a fundamental role in the development and the transmission of ailment. Anti-midgut antibodies show the extensive variety of activity, blockading the development of pathogen in various species of mosquitoes. In addition to reducing the egg-laying ability of mosquitoes and survivorship also block the transmission activity of pathogen. Mitsuhashi and Maramorosch media was used to culture the mosquito midgut cells. The cells were formalin inactivated and injected into the rabbits in plain and adjuvanted form to raise hyperimmune serum. The serum was processed for IHA and serum showing high titre were selected for blood feeding assay. The blood from the rabbits was fed to the mosquitos to observe the mosquitocidal effect of the antigen. In blood feeding assay killing of mosquitoes was also observed after regular interval of time. The overall results proved that mosquito midgut contains antigenic peptides that may be able to induce the antibody response. These antigenic peptides somehow irritate digestive mucosa of the mosquitoes on blood feeding and have the potential to kill or reduce the mosquito population.
蚊子传播的疾病可以借助具有杀蚊或灭蚊作用的疫苗大大减少。蚊子的中肠在疾病的发展和传播中起着重要的作用。抗中肠抗体表现出广泛多样的活性,阻断各种蚊子病原体的发展。除降低蚊子的产卵能力和存活能力外,还阻断了病原体的传播活动。采用Mitsuhashi和Maramorosch培养基培养蚊子中肠细胞。用福尔马林灭活细胞,以普通和佐剂的形式注射兔体内,提高高免疫血清。对血清进行IHA处理,选取高滴度血清进行血喂养试验。取兔血喂蚊,观察抗原的灭蚊效果。采血实验中,按一定时间间隔观察蚊虫的杀灭情况。结果表明,蚊子中肠中含有抗原肽,可能具有诱导抗体应答的作用。这些抗原肽以某种方式刺激吸血蚊子的消化粘膜,具有杀死或减少蚊子数量的潜力。
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引用次数: 1
Identification of ω-sites of Glycosylphosphatidylinositol Anchored Proteins 糖基磷脂酰肌醇锚定蛋白ω-位点的鉴定
Pub Date : 2018-09-01 DOI: 10.29245/2578-3009/2018/5.1151
Yusuke Masuishi, Shota Endo, H. Kasuga, Tomoo Hidaka, T. Kakamu, Hikariga-oka, Fukushima City
Unique and complex post-translational modifications are present in the outer leaflet of the plasma membrane. Glycosylphosphatidylinositol (GPI) anchoring is essential for the expression of several outer membrane proteins on the cell surface. A common GPI anchor structure is constituted by glycan moiety, lipid moiety, phosphate and ethanolamine. GPI-anchored proteins (GPI-APs) are observed among eukaryotic species. Abnormal GPI anchoring of proteins is thought to cause various diseases such as paroxysmal nocturnal hemoglobinuria. Recently, many inherited GPI deficiencies (IGDs) have been reported to cause epilepsy, mental retardation, coarse facial features, and multiple organ anomalies. Diseases caused by abnormal GPI anchoring will probably continue to increase, because it is still unknown how many causative genes of IGDs are present. Therefore, in order to study these diseases, the analytical methods of GPI-APs will become important in the future. To date, many methods have been developed for analysis of GPI- APs. In this review, we attempt to summarize the present knowledge about comprehensive analytical methods of GPI-APs and introduce briefly some GPI anchor-related diseases.
独特而复杂的翻译后修饰存在于质膜的外小叶中。糖基磷脂酰肌醇(GPI)锚定对细胞表面几种外膜蛋白的表达至关重要。常见的GPI锚定结构由糖基、脂基、磷酸基和乙醇胺组成。gpi锚定蛋白(GPI-APs)在真核生物物种中被观察到。异常的GPI锚定蛋白被认为可引起各种疾病,如阵发性夜间血红蛋白尿。最近,许多遗传性GPI缺陷(IGDs)被报道导致癫痫、智力迟钝、面部粗糙和多器官异常。异常GPI锚定引起的疾病可能会继续增加,因为仍然不知道有多少igd致病基因存在。因此,为了研究这些疾病,GPI-APs的分析方法将在未来变得重要。迄今为止,已经开发了许多分析GPI- ap的方法。在本文中,我们试图总结目前关于GPI- aps综合分析方法的知识,并简要介绍一些GPI锚定相关疾病。
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引用次数: 0
Pathological Approach to A Special Benign Tumor with Regard to its Differential Diagnose Spectrum: Intranodal Palisaded Myofibroblastoma 一种特殊良性肿瘤的病理诊断:结内栅栏性肌成纤维细胞瘤
Pub Date : 2018-09-01 DOI: 10.29245/2578-3009/2018/5.1158
Y. Karabulut
Intranodal palisaded myofibroblastoma, also known as “intranodal hemorrhagic spindle cell tumor with amianthoid fibers,” is a benign mesenchymal tumor of the lymph node originating from smooth muscle cells and myofibroblasts often with the presence of amianthoid fibers. Ninety-three cases of intranodal palisaded myofibroblastoma have been reported in the literatüre since its first description and most of them have the same clinical history “painless firm nodüle”. It is mostly seen in inguinal region there are few cases have been described in other locations. It’s large and important differential diagnostic spectrum makes this tumor special. Pathological Approach to A Special Benign Tumor with Regard to its Differential Diagnose Spectrum: Intranodal Palisaded Myofibroblastoma
结内栅栏性肌纤维母细胞瘤,又称结内出血性梭形细胞瘤,是一种良性的淋巴结间充质肿瘤,起源于平滑肌细胞和肌成纤维细胞,常伴有肌样纤维。自首次描述以来,文献报道了93例结内栅栏性肌成纤维细胞瘤,其中大多数具有相同的临床病史“无痛性硬结节”。它多见于腹股沟区,其他部位的病例很少。它巨大而重要的鉴别诊断谱使得这个肿瘤很特别。一种特殊良性肿瘤的病理诊断:结内栅栏性肌成纤维细胞瘤
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引用次数: 0
Expression, Functions, and Treatment Target of PD-L1 (B7-H1) in Multiple Myeloma PD-L1 (B7-H1)在多发性骨髓瘤中的表达、功能及治疗靶点
Pub Date : 2018-09-01 DOI: 10.29245/2578-3009/2018/5.1162
H. Tamura, M. Ishibashi, Mika Sunakawa, Hidemi Takahashi
Programmed death ligand 1 (PD-L1) expression on myeloma cells is induced by JAK2, STAT3, and MEK1/2-mediated interleukin-6 signaling, a strong inducer of PD-L1 interferon-γ produced by T and natural killer cells, and APRIL produced by osteoclasts in the tumor microenvironment. The soluble form of PD-L1, derived from extracellular domains of PD-L1 molecules expressed in the tumor environment, may also contribute to tumor immune evasion. PD-L1-expressing myeloma cells not only have the ability to escape from the attack of tumor-specific T cells but also high proliferation potential. Furthermore, PD-L1 on myeloma cells delivers a reverse signal to tumor cells through PD-1 binding, resulting in the phosphorylation of Akt accompanied by the acquisition of resistance to anti-myeloma agents. Based on the function of PD-L1 in myeloma, the blockade of the PD-1–PD-L1 pathway is a reasonable treatment in refractory patients. Phase I/II clinical trials of anti-PD-1 antibody combined with immunomodulatory drugs demonstrated excellent effects in heavily pretreated multiple myeloma patients with acceptable tolerability. The timing and combination drug of anti-PD-1/PD-L1 antibodies should be considered to improve clinical effects with low mortality in refractory myeloma patients.
程序性死亡配体1 (PD-L1)在骨髓瘤细胞上的表达是由JAK2、STAT3和mek1 /2介导的白介素-6信号传导诱导的,白介素-6是T细胞和自然杀伤细胞产生PD-L1干扰素-γ的强诱导剂,肿瘤微环境中破骨细胞产生APRIL。PD-L1的可溶性形式来源于肿瘤环境中表达的PD-L1分子的细胞外结构域,也可能有助于肿瘤免疫逃避。表达pd - l1的骨髓瘤细胞不仅具有逃避肿瘤特异性T细胞攻击的能力,而且具有较高的增殖潜能。此外,骨髓瘤细胞上的PD-L1通过PD-1结合向肿瘤细胞传递反向信号,导致Akt磷酸化,同时获得对抗骨髓瘤药物的耐药性。基于PD-L1在骨髓瘤中的功能,阻断PD-1-PD-L1通路是难治性患者的合理治疗方法。抗pd -1抗体联合免疫调节药物的I/II期临床试验在重度预处理的多发性骨髓瘤患者中显示出良好的疗效,耐受性可接受。对于难治性骨髓瘤患者,应考虑抗pd -1/PD-L1抗体的时机和联合用药,以提高临床疗效,降低死亡率。
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引用次数: 0
Emerging Evidence Supports the Hypothesis that Neutrophil Extracellular Traps are a Major Factor in Genesis and Progression of Chronic Obstructive Pulmonary Disease 新出现的证据支持中性粒细胞胞外陷阱是慢性阻塞性肺疾病发生和发展的主要因素的假设
Pub Date : 2018-09-01 DOI: 10.29245/2578-3009/2018/5.1161
A. Obermayer, W. Stoiber, Fikreta Grabcanovic-Musija, Salzburg, Austria
Since their discovery about fifteen years ago, neutrophil extracellular traps (NETs) have been recognized as an intrinsic part of vertebrate innate immunity and inflammatory response. Consisting of entangled strands of extracellular DNA decorated with histones, elastase, myeloperoxidase and other proteins, NETs entrap and kill pathogens, but are increasingly also found to contribute to acute and chronic inflammatory disease due to their toxicity to host cell and autoimmunity induction. Chronic obstructive pulmonary disease (COPD) turned out to be among the major disorders involving overshooting formation of NETs and associated adverse effect. In the present review, we summarize the progress in knowledge on the role of NETs in COPD pathology made since our first reports on this subject. We highlight recent substantial advances and discuss possible cause-and-effect relationships, connections with common comorbidities and interactions with drugs, also to illustrate the importance of NETs as a future diagnostic tool and target for new medication strategies.
自从15年前被发现以来,中性粒细胞胞外陷阱(NETs)已被认为是脊椎动物先天免疫和炎症反应的内在组成部分。net由缠绕在一起的细胞外DNA链组成,由组蛋白、弹性酶、髓过氧化物酶和其他蛋白质修饰,可以诱杀病原体,但由于其对宿主细胞的毒性和自身免疫诱导,也越来越多地被发现有助于急性和慢性炎症性疾病。慢性阻塞性肺疾病(COPD)被证明是NETs过度形成和相关不良反应的主要疾病之一。在本综述中,我们总结了自我们首次报道这一主题以来,关于NETs在COPD病理中的作用的知识进展。我们强调了最近的重大进展,并讨论了可能的因果关系,与常见合并症的联系以及与药物的相互作用,也说明了NETs作为未来诊断工具和新药物策略目标的重要性。
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引用次数: 1
A Flow Cytometric Immunophenotyping Approach to the Detection of Regulated Cell Death Processes 流式细胞术免疫分型方法检测调节细胞死亡过程
Pub Date : 2018-09-01 DOI: 10.29245/2578-3009/2018/5.1159
G. Warnes
The use of the Western Blot technique has been the gold standard to determine protein expression and to semi-quantitate this expression in cell lysates. The recent publication of a flow cytometric immunophenotyping method employing fluorescently labelled antibodies to the intracellular labelling of antigens involved in Regulated Cell Death (RCD) processes has allowed the detection of three of these processes simultaneously which gave clarity to the interpretation of the relationship between apoptosis, RIP1 dependent apoptosis and necroptosis. Flow cytometry can now immunophenotype necroptosis by virtue of the up-regulation of RIP3 with simultaneous estimations of the degree of classic apoptosis (Caspase-3+ve/RIP3ve) and of RIP1-dependent apoptosis (Caspase-3+ve/RIP3+ve) in live and dead cell populations. This approach for detecting multiple forms of cell death has been confirmed by the use of apoptosis and necroptosis blocking agents, zVAD and necrostatin-1 after treatment with etoposide or shikonin which induced apoptosis and necroptosis. The addition of anti-PARP and H2AX antibodies for the detection of parthanatos and DNA damage showed that double negative Caspase-3-ve/RIP3-ve cells detected in a previous study have undergone parthanatos or still display a negative phenotype for any cell death process. A Flow Cytometric Immunophenotyping Approach to the Detection of Regulated Cell Death Processes A Vossenkamper1, G Warnes2* 1Centre for Immunobiology, The Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary London University, 4 Newark Street, London E1 2AT, UK 2Flow Cytometry Core Facility, The Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary London University, 4 Newark Street, London E1 2AT, UK
Western Blot技术一直是测定蛋白表达和半定量细胞裂解物中蛋白表达的金标准。最近发表的流式细胞术免疫分型方法采用荧光标记抗体对参与调节细胞死亡(RCD)过程的抗原进行细胞内标记,可以同时检测到其中的三个过程,从而清楚地解释了细胞凋亡、RIP1依赖性细胞凋亡和坏死坏死之间的关系。流式细胞术现在可以通过上调RIP3来免疫表型坏死,同时估计活细胞和死细胞群体中的经典凋亡(Caspase-3+ve/RIP3ve)和rip1依赖性凋亡(Caspase-3+ve/RIP3+ve)的程度。这种检测多种形式细胞死亡的方法已经通过使用凋亡和坏死坏死阻断剂,zVAD和坏死他汀-1在用乙草苷或紫草素治疗后诱导细胞凋亡和坏死坏死得到证实。加入抗parp和H2AX抗体检测parthanatos和DNA损伤表明,先前研究中检测到的双阴性Caspase-3-ve/RIP3-ve细胞发生了parthanatos或在任何细胞死亡过程中仍表现为阴性表型。A Vossenkamper1, G Warnes2* 1免疫生物学中心,暴雪研究所,Barts和伦敦医学和牙科学院,玛丽女王伦敦大学,4 Newark Street, London E1 2AT, UK 2流式细胞术核心设施,暴雪研究所,Barts和伦敦医学和牙科学院,玛丽女王伦敦大学,4 Newark Street, London E1 2AT, UK
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引用次数: 6
Congenital Rubella Syndrome in The African Region - Data from Sentinel Surveillance. 非洲地区先天性风疹综合征-哨点监测数据。
Pub Date : 2018-08-02
Balcha Masresha, Messeret Shibeshi, Reinhard Kaiser, Richard Luce, Regis Katsande, Richard Mihigo

Introduction: Rubella is a mild febrile rash illness caused by the rubella virus. The most serious consequence of rubella is congenital rubella syndrome (CRS), which occurs if the primary rubella infection occurs during early pregnancy, with subsequent infection of the placenta and the developing fetus.

Methods: WHO supported countries to set up sentinel surveillance for CRS using standard case definitions, protocols, and case classification scheme. This descriptive analysis summarises the data from 5 countries which have been regularly reporting.

Results: A total of 383 suspected cases of CRS were notified from the 5 countries as of December 2016, of which 52 cases were laboratory confirmed and 67 were confirmed on clinical grounds.The majority (43%) of confirmed CRS cases were in the age group 6 - 11 months. The most common major clinical manifestation (Group A) among the confirmed cases is congenital heart disease (72%) followed by cataracts (32%) and glaucoma (10%).

Discussion and conclusions: The number of years of reporting from these sentinel sites is too short to describe trends in CRS occurrence across the years. However, the limited surveillance data has yielded comparable information with other developing countries prior to introduction of rubella vaccine. As more countries introduce rubella vaccine into their immunisation programs, there is a need to ensure that all rubella outbreaks are thoroughly investigated and documented, to expand sentinel surveillance for CRS in more countries in the Region, and to complement this with retrospective record reviews for CRS cases in selected countries.

简介:风疹是一种由风疹病毒引起的轻度发热性皮疹疾病。风疹最严重的后果是先天性风疹综合征(CRS),如果在妊娠早期发生原发性风疹感染,随后会感染胎盘和发育中的胎儿。方法:世卫组织支持各国使用标准病例定义、方案和病例分类方案建立CRS哨点监测。这一描述性分析总结了定期报告的5个国家的数据。结果:截至2016年12月,5国共通报疑似CRS病例383例,其中实验室确诊52例,临床确诊67例。大多数确诊的CRS病例(43%)发生在6 - 11个月的年龄组。确诊病例中最常见的主要临床表现(A组)是先天性心脏病(72%),其次是白内障(32%)和青光眼(10%)。讨论和结论:这些哨点报告的年数太短,无法描述历年CRS发生的趋势。然而,在引入风疹疫苗之前,有限的监测数据产生了与其他发展中国家可比的信息。随着越来越多的国家将风疹疫苗纳入其免疫规划,有必要确保对所有风疹疫情进行彻底调查和记录,在本区域更多国家扩大风疹综合征的哨点监测,并在选定国家对风疹综合征病例进行回顾性记录审查。
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引用次数: 0
Five-year experience of African Vaccination Week implemented by the WHO Regional Office. 世界卫生组织区域办事处实施非洲疫苗接种周五年经验。
Pub Date : 2018-08-02
Joseph Okeibunor, Richard Mihigo, Blanche Anya, Felicitas Zawaira

The 5th edition of the African Vaccination Week (AVW) kicked off in Lusaka, Zambia, on 23 April 2016, the same day as did the 4th World Immunization Week (WIW), and vaccination week in other WHO regions. The theme was "Save lives, prevent disabilities, vaccinate!". The aim was to draw attention to the need to attain universal immunization coverage in the African Region by closing the immunization gap, while also celebrating the important polio eradication milestone reached in the African Region. Twenty-eight (59.6%) of the 47 countries in the African Region celebrated the AVW within the regionally set dates of 24th to 30th April 2015. However, given its flexibility, the celebration continued until September in 15 other countries in the Region. Three countries, namely Comoros, Gabon, and Cape Verde did not join the celebration for the 2015 edition of the AVW. Countries used the opportunity to introduce new vaccines into their routine immunization. Populations, hitherto unreached with basic health services were reached with needed services, such as vitamin A supplementation, deworming, and catch up immunization services. The programmes promoted awareness of the benefits of vaccines and the rights of communities to demand vaccines and immunization services to save lives and prevent disabilities. The number of participating countries rose steadily from 40 in 2011 to 43 and 46 countries in 2013 and 2014 respectively. The number ranged from one intervention integrated with AVW in 17 countries to 5 interventions integrated with the AVW in three countries. In 2015, 67.4% of the participating countries integrated other interventions with AVW activities.

第五届非洲疫苗接种周(AVW)于2016年4月23日在赞比亚卢萨卡拉开帷幕,与第四届世界免疫周(WIW)和世界卫生组织其他地区的疫苗接种周同一天。主题是“拯救生命,预防残疾,接种疫苗!”。其目的是提请注意通过缩小免疫差距在非洲区域实现全民免疫覆盖的必要性,同时也庆祝非洲区域实现了重要的脊髓灰质炎根除里程碑。非洲地区47个国家中有28个(59.6%)在2015年4月24日至30日的区域规定日期内庆祝AVW。然而,鉴于其灵活性,该地区其他15个国家的庆祝活动一直持续到9月。科摩罗、加蓬和佛得角三个国家没有参加2015年版AVW的庆祝活动。各国利用这一机会将新疫苗引入其常规免疫。迄今为止尚未获得基本卫生服务的人群获得了所需的服务,如补充维生素A、驱虫和补充免疫服务。这些方案提高了人们对疫苗的益处以及社区要求疫苗和免疫服务以拯救生命和预防残疾的权利的认识。参与国数量从2011年的40个稳步增加到2013年和2014年的43个和46个。这一数字从17个国家的一项与AVW相结合的干预措施到3个国家的5项与AVV相结合的介入措施不等。2015年,67.4%的参与国将其他干预措施与AVW活动相结合。
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引用次数: 0
期刊
Journal of immunological sciences
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