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Measuring Mouse Somatosensory Reflexive Behaviors with High-speed Videography, Statistical Modeling, and Machine Learning. 用高速摄像、统计建模和机器学习测量小鼠体感反射行为。
Neuromethods
Pub Date : 2022-01-01 Epub Date: 2022-05-27 DOI: 10.1007/978-1-0716-2039-7_21
Ishmail Abdus-Saboor, Wenqin Luo

Objectively measuring and interpreting an animal's sensory experience remains a challenging task. This is particularly true when using preclinical rodent models to study pain mechanisms and screen for potential new pain treatment reagents. How to determine their pain states in a precise and unbiased manner is a hurdle that the field will need to overcome. Here, we describe our efforts to measure mouse somatosensory reflexive behaviors with greatly improved precision by high-speed video imaging. We describe how coupling sub-second ethograms of reflexive behaviors with a statistical reduction method and supervised machine learning can be used to create a more objective quantitative mouse "pain scale." Our goal is to provide the readers with a protocol of how to integrate some of the new tools described here with currently used mechanical somatosensory assays, while discussing the advantages and limitations of this new approach.

客观地测量和解释动物的感官体验仍然是一项具有挑战性的任务。当使用临床前啮齿动物模型来研究疼痛机制和筛选潜在的新疼痛治疗试剂时，情况尤其如此。如何以精确和公正的方式确定他们的疼痛状态是该领域需要克服的障碍。在这里，我们描述了我们通过高速视频成像测量小鼠体感反射行为的努力，该行为的精度大大提高。我们描述了如何将反射行为的亚秒行为图与统计归约方法和监督机器学习相结合，以创建更客观的定量小鼠“疼痛量表”。我们的目标是为读者提供一个协议，说明如何将本文描述的一些新工具与当前使用的机械体感测定相结合，同时讨论了这种新方法的优点和局限性。
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引用次数: 1
Pou4f3DTR Mice Enable Selective and Timed Ablation of Hair Cells in Postnatal Mice Pou4f3DTR小鼠能够选择性和定时消融出生后小鼠的毛细胞
Neuromethods
Pub Date : 2022-01-01 DOI: 10.1007/978-1-0716-2022-9_1
J. Stone, E. Rubel, M. Warchol
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引用次数: 0
Ribosomal Pulldown Assays and Their Use to Analyze Gene Expression in Multiple Inner Ear Cell Types 核糖体下拉试验及其在多种内耳细胞类型基因表达分析中的应用
Neuromethods
Pub Date : 2022-01-01 DOI: 10.1007/978-1-0716-2022-9_8
Maggie S. Matern, B. Milon, R. Elkon, R. Hertzano
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引用次数: 0
Learning Paradigms and Genetic Tools for the Study of Cerebellum-Dependent Learning and Memory 小脑依赖性学习和记忆研究的学习范式和遗传工具
Neuromethods
Pub Date : 2022-01-01 DOI: 10.1007/978-1-0716-2026-7_6
A. Katoh
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引用次数: 0
Transcranial Direct Current Stimulation for the Treatment of Major Depressive Disorder 经颅直流电刺激治疗重度抑郁症
Neuromethods
Pub Date : 2022-01-01 DOI: 10.1007/978-1-0716-2083-0_16
Lucas Borrione, Laís B. Razza, A. Moffa, A. Brunoni
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引用次数: 0
Antidepressants, Sexual Behavior, and Translational Models for Male Sexual Dysfunction: Development of Animal Models, Pharmacology, and Genetics 抗抑郁药、性行为和男性性功能障碍的转化模型:动物模型、药理学和遗传学的发展
Neuromethods
Pub Date : 2022-01-01 DOI: 10.1007/978-1-0716-2083-0_20
J. Olivier, J. Janssen, T. Pattij, Stephen de Prêtre, B. Olivier
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引用次数: 2
Correlative Light and Electron Microscopy (CLEM): Bringing Together the Best of Both Worlds to Study Neuronal Autophagy 相关光学和电子显微镜(CLEM):结合两个世界的最好的研究神经元自噬
Neuromethods
Pub Date : 2021-08-20 DOI: 10.1007/978-1-0716-1589-8_10
J. Kriel, D. Lumkwana, L. Joubert, Martin L. Jones, C. Peddie, L. Collinson, B. Loos, L. Engelbrecht
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引用次数: 0
Measuring 测量
Neuromethods
Pub Date : 2021-08-20 DOI: 10.1007/978-1-0716-1589-8_6
Andrea Du Toit, J. S. Hofmeyr, Benjamin Loos
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引用次数: 0
Quantification of the Neurofilament Light Chain Protein by Single Molecule Array (Simoa) Assay 单分子阵列(Simoa)法测定神经丝轻链蛋白
Neuromethods
Pub Date : 2021-01-01 DOI: 10.1007/978-1-0716-1319-1_13
C. Barro, Sarah Storz, S. Gröbke, Z. Michalak, J. Kuhle
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引用次数: 0
Intracranial Blister Aneurysms 颅内水疱动脉瘤
Neuromethods
Pub Date : 2021-01-01 DOI: 10.1007/978-1-0716-1530-0_9
A. Alturki, A. Thomas, C. Ogilvy
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引用次数: 0
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