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Case studies from the experience of early career researchers in East Africa in building community engagement in research 来自东非早期职业研究人员在建立社区参与研究方面经验的案例研究
Pub Date : 2022-06-27 DOI: 10.12688/openresafrica.13349.2
Joel L. Bargul, Denna M. Mkwashapi, I. Namagembe, Immaculate Nakityo, A. Nakimuli, J. Byamugisha, Daniel Semakula, J. Seeley, N. Sewankambo
Background: In this paper, we explain how three early career researchers actively engaged community members in their health research projects in Kenya, Tanzania and Uganda, and what was learnt from the experience. The research project in Kenya was on camel trypanosomiasis and the role of camel biting keds (or louse flies) in disease transmission. The project in Tanzania looked at the effect of human immunodeficiency virus and antiretroviral therapy on fertility and ascertained the trends in the use of family planning services amongst women of reproductive age. The focus of the project in Uganda was the implementation of maternal death surveillance and the response policy to determine the cause of maternal deaths and how they might be prevented. Methods: In the three different settings, efforts to ensure local community engagement provided a focus for the researchers to hone their skills in explaining research concepts and working in partnership with community members to co-develop ideas, their research methods and outputs. Results: Involvement of communities in scientific research, which entailed a two-way mutual engagement process, led to (i) generation of new research ideas that shaped the work, (ii) strengthened mutual trust, and (iii) promoted uptake of research findings. Conclusion: Our key findings strongly support the need for considering community engagement as one of the key components in research studies.
背景:在本文中,我们解释了三位早期职业研究人员在肯尼亚、坦桑尼亚和乌干达如何积极地让社区成员参与他们的健康研究项目,以及从经验中学到了什么。肯尼亚的研究项目是关于骆驼锥虫病和骆驼咬蝇(或虱子蝇)在疾病传播中的作用。坦桑尼亚的项目研究了人体免疫缺陷病毒和抗逆转录病毒疗法对生育力的影响,并查明了育龄妇女使用计划生育服务的趋势。乌干达项目的重点是实施产妇死亡监测和应对政策,以确定产妇死亡的原因以及如何预防产妇死亡。方法:在三种不同的环境中,确保当地社区参与的努力为研究人员提供了一个重点,以磨练他们在解释研究概念和与社区成员合作以共同发展思想、研究方法和产出方面的技能。结果:社区参与科学研究,这需要一个双向的相互参与过程,导致(i)产生新的研究思想,塑造了工作,(ii)加强了相互信任,(iii)促进了研究成果的吸收。结论:我们的主要发现强烈支持将社区参与作为研究的关键组成部分之一的必要性。
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引用次数: 1
Maize seeds storage systems and post-harvest losses in Benin: diversity, efficiency, storage insects, and implications for better products conservation 贝宁玉米种子储存系统和收获后损失:多样性、效率、储存昆虫以及对更好地保护产品的影响
Pub Date : 2022-06-20 DOI: 10.12688/openresafrica.13372.1
Gabin Samba, A. Dassou, R. Idohou, Corinne M. Anagonou, A. Dansi
Background: Storage pests cause extensive damages to stored products and are responsible for huge post-harvest losses affecting the quality, quantity, and germination potential of stored grains and seeds. This study aimed to investigate the variability of traditional methods of storage and conservation of maize seeds practiced by farmers to propose alternative measures for a significant reduction of post-harvest losses of seeds. Methods : Using participatory research approaches, we surveyed farmers from 21 randomly selected villages in 5 districts in southern Benin. Data were collected on the storage structures of the 3 certified and most produced maize seeds varieties. The forms under which maize seeds are stored, as well as the damage caused by the major storage insects, were determined. Results: Results showed that most farmers store maize seeds in the form of grains and spathe. Following the laboratory observation of the three maize varieties studied, the DMR / QPM variety produced only in the district of Zagnanado has a low abundance of storage insects and a low rate of post-harvest losses. Sitophilus zeamais is the most abundant pest of the three maize seed varieties followed by Prostephanus truncatus. The variety 2000 SYN EE was the most attacked by storage insects. The most promising post-harvest agricultural practice is the storage of maize with spathe saved in jute bags, in granaries or cribs. Conclusions: Storage insects contribute to the depreciation of the quality of grains, loss of grain and reduction of their germinability in stock. Improving farmers' awareness of these post-harvest practices could help to reduce the damage of storage insects.
背景:储粮害虫对储粮产品造成广泛的危害,对储粮和种子的质量、数量和发芽潜力造成巨大的采后损失。本研究旨在调查农民使用的传统玉米种子储存和保存方法的可变性,以提出显著减少种子收获后损失的替代措施。方法:采用参与式研究方法,对贝宁南部5个区随机抽取的21个村庄的农民进行调查。收集了3个经认证和产量最高的玉米种子品种的储存结构数据。确定了玉米种子的贮藏形式,以及主要贮藏昆虫对玉米种子造成的危害。结果:结果表明,大多数农民以谷粒和芽的形式储存玉米种子。通过对所研究的三个玉米品种的实验室观察,仅在Zagnanado地区生产的DMR / QPM品种的贮藏昆虫丰度低,收获后损失率低。玉米象是3个玉米种子品种中数量最多的害虫,其次是长角螟。品种2000 SYN EE是贮藏昆虫侵害最多的品种。最有希望的收获后农业做法是将玉米用黄麻袋、粮仓或摇篮储存起来。结论:储粮昆虫是造成粮食品质下降、粮食损失和粮食发芽率降低的原因之一。提高农民对这些收获后做法的认识有助于减少贮藏昆虫的损害。
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引用次数: 0
NOMAD: metagenomic characterisation of the viral pathogen composition in outbreaks of non-malaria acute febrile illness cases NOMAD:非疟疾急性发热性疾病暴发中病毒病原体组成的宏基因组特征
Pub Date : 2022-06-09 DOI: 10.12688/openresafrica.13406.1
Benard W. Kulohoma, Ibrahim Ng'eno
The clinical importance of non-malaria febrile acute illness (NM-AFI) in patients with a negative parasitological test has become apparent, with the progressive reduction in malaria transmission in endemic regions. Bacterial pathogens, for example Streptococcus pneumoniae and Haemophilus influenzae, which contribute disproportionally to febrile illness, are now preventable by vaccines. However, there are no vaccines, and little is known about viral NM-AFI prevalence, proliferation, virulence, and transmission chains between hosts. Although the predominant viral causes of NM-AFI are established, it is unclear if there are other NM-AFI associated emerging infectious viral pathogens that previously remained undetectable by conventional diagnostic strategies, for example severe acute respiratory syndrome coronavirus 2 (SARS-CoV-​2). Presumptive broad-spectrum antibiotic prescriptions to aparasitaemic patients not only drive drug resistance, but also lead to poor treatment outcomes. We hypothesized that insights on NM-AFI etiology, and consequently case management, could be improved by exploiting viral sequence diversity to identify viral pathogens present within metagenomics samples. We exploited simulated and existing infectious disease (Ebola, hepatitis C, chikungunya, and mosquito-borne arboviruses) metagenomic datasets to determine the composition of viral pathogens present, by implementing profile Hidden Markov Models derived from Swiss-Prot viral reference sequences for accurate pathogen detection and classification. Our analysis identified a combination of sequences from multiple viral etiological agents within the same disease sample. This approach provides a granular perspective of multiple viral etiological agents present within a single intra-host disease episode. It highlights prevalent viral strains that can subsequently be routinely detected using directed diagnostic tests to improve disease surveillance in endemic regions.
随着疟疾流行地区传播的逐渐减少,寄生虫学检测呈阴性的患者中非疟疾热性急性疾病(NM-AFI)的临床重要性已经变得明显。细菌病原体,如肺炎链球菌和流感嗜血杆菌,对发热性疾病的贡献不成比例,现在可以通过疫苗预防。然而,目前还没有疫苗,对病毒NM-AFI的流行率、增殖、毒力和宿主之间的传播链知之甚少。尽管NM-AFI的主要病毒病因已经确定,但尚不清楚是否存在其他与NM-AFI相关的新出现的传染性病毒病原体,这些病原体以前仍无法通过常规诊断策略检测到,例如严重急性呼吸综合征冠状病毒2型(严重急性呼吸系统综合征冠状病毒-​2) 。假定对无寄生虫血症患者开具广谱抗生素处方不仅会导致耐药性,还会导致不良的治疗结果。我们假设,通过利用病毒序列多样性来识别宏基因组学样本中存在的病毒病原体,可以提高对NM-AFI病因的认识,从而改善病例管理。我们利用模拟和现有的传染病(埃博拉、丙型肝炎、基孔肯雅病毒和蚊媒虫媒病毒)宏基因组数据集,通过实施源自Swiss-Prot病毒参考序列的轮廓隐马尔可夫模型来确定存在的病毒病原体的组分,以进行准确的病原体检测和分类。我们的分析确定了同一疾病样本中多种病毒病原体的序列组合。这种方法提供了在单个宿主内疾病发作中存在的多种病毒病原体的细粒度视角。它强调了流行的病毒株,随后可以使用定向诊断测试进行常规检测,以改善流行地区的疾病监测。
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引用次数: 0
Cost reduction strategies in the remediation of petroleum hydrocarbon contaminated soil. 降低石油烃污染土壤修复成本的策略。
Pub Date : 2022-04-08 eCollection Date: 2022-01-01 DOI: 10.12688/openresafrica.13383.1
Ismail B Ahmed, Eucharia O Nwaichi, Ejikeme Ugwoha, John N Ugbebor, Samuel B Arokoyu

Petroleum hydrocarbon spill on land pollutes soil and reduces its ecosystem. Hydrocarbon transport in the soil is aided by several biological, physical, and chemical processes. However, pore characteristics play a major role in the distribution within the soil matrix. Restoring land use after spills necessitates remediation using cost-effective technologies. Several remediation technologies have been demonstrated at different scales, and research is ongoing to improve their performances towards the reduction of treatment costs. The process of removing the contaminants in the soil is through one or a combination of containment, separation, and degradation methods under the influence of biological, physical, chemical, and electrically-dominated processes. Generally, performance improvement is achieved through the introduction of products/materials and/or energy. Nevertheless, the technologies can be categorized based on effectiveness period as short, medium, and long term. The treatment cost of short, medium, and long-term technologies are usually in the range of $39 - 331/t (/tonne), $22 - 131/t, and $8 - 131/t, respectively. However, the total cost depends on other factors such as site location, capital cost, and permitting. This review compiles cost-saving strategies reported for different techniques used in remediating petroleum hydrocarbon polluted soil. We discuss the principles of contaminant removal, performance enhancing methods, and the cost-effectiveness analysis of selected technologies.

石油碳氢化合物泄漏到土地上会污染土壤并破坏其生态系统。碳氢化合物在土壤中的迁移受多种生物、物理和化学过程的影响。然而,孔隙特征在土壤基质内的分布中起着重要作用。要在泄漏后恢复土地的使用,就必须采用具有成本效益的技术进行修复。目前已在不同范围内展示了几种修复技术,并正在进行研究,以提高这些技术的性能,降低处理成本。清除土壤中污染物的过程是在生物、物理、化学和电主导过程的影响下,通过一种或多种遏制、分离和降解方法进行的。一般来说,通过引入产品/材料和/或能源来实现性能改善。不过,这些技术可根据有效期分为短期、中期和长期。短期、中期和长期技术的处理成本通常分别在 39 - 331 美元/吨(/吨)、22 - 131 美元/吨和 8 - 131 美元/吨之间。然而,总成本取决于其他因素,如地点、资本成本和许可。本综述汇编了用于修复石油烃污染土壤的不同技术的成本节约策略。我们讨论了污染物去除原理、性能提升方法以及选定技术的成本效益分析。
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引用次数: 0
Farmers' knowledge, perceptions, and practices on animal trypanosomosis and the tsetse fly vector: A cross-sectional study around Kenya's Arabuko-Sokoke Forest Reserve at the livestock-wildlife interface. 农民对动物锥虫病和采采蝇病媒的知识、认知和做法:一项围绕肯尼亚阿拉布科-索科克森林保护区牲畜-野生动物界面的横断面研究。
Pub Date : 2022-01-01 DOI: 10.12688/openresafrica.13397.1
Erick K Serem, Joel L Bargul, Moses M Ngari, Osman A Abdullahi, David M Mburu

Background: Animal African trypanosomosis (AAT) is a veterinary disease caused by trypanosomes transmitted cyclically by tsetse flies. AAT causes huge agricultural losses in sub-Saharan Africa. Both tsetse flies and trypanosomosis (T&T) are endemic in the study area inhabited by smallholder livestock farmers at the livestock-wildlife interface around Arabuko-Sokoke Forest Reserve (ASFR) in Kilifi County on the Kenyan coast. We assessed farmers' knowledge, perceptions and control practices towards T&T. Methods: A cross-sectional study was conducted during November and December 2017 to collect data from 404 randomly selected cattle-rearing households using a structured questionnaire. Descriptive statistics were used to determine farmers' knowledge, perceptions, and control practices towards T&T. Demographic factors associated with knowledge of T&T were assessed using a logistic regression model. Results: Participants consisted of 53% female, 77% married, 30% elderly (>55 years), and the majority (81%) had attained primary education or below. Most small-scale farmers (98%) knew the tsetse fly by its local name, and 76% could describe the morphology of the adult tsetse fly by size in comparison to the housefly's ( Musca domestica). Only 16% of the farmers knew tsetse flies as vectors of livestock diseases. Higher chances of adequate knowledge on T&T were associated with the participants' (i) age of 15-24 years (aOR 2.88 (95% CI 1.10-7.52), (ii) level of education including secondary (aOR 2.46 (95% CI 1.43-4.24)) and tertiary (aOR 3.80 (95% CI 1.54-9.37)), and (iii) employment status: self-employed farmers (aOR 6.54 (95% CI 4.36-9.80)). Conclusions: Our findings suggest that small-scale farmers around ASFR have limited knowledge of T&T. It is envisaged that efforts geared towards training of the farmers would bridge this knowledge gap and sharpen the perceptions and disease control tactics to contribute to the prevention and control of T&T.

背景:非洲动物锥虫病(AAT)是一种由采采蝇周期性传播的锥虫引起的兽医学疾病。AAT在撒哈拉以南非洲造成了巨大的农业损失。采采蝇和锥虫病(T&T)在肯尼亚海岸基利菲县阿拉布科-索科克森林保护区(ASFR)附近牲畜-野生动物交界的小农养殖户居住的研究区域流行。我们评估了农民对T&T的知识、认知和控制做法。方法:采用横断面研究方法,于2017年11月至12月随机抽取404户养牛户,采用结构化问卷收集数据。描述性统计用于确定农民对T&T的知识,观念和控制措施。使用逻辑回归模型评估与T&T知识相关的人口统计学因素。结果:参与者中53%为女性,77%为已婚,30%为老年人(>55岁),大多数(81%)为初等及以下文化程度。大多数小农(98%)知道采采蝇的当地名称,76%的人可以通过大小描述成年采采蝇与家蝇(Musca domestica)的形态。只有16%的农民知道采采蝇是牲畜疾病的媒介。对T&T有充分了解的机会与参与者(i) 15-24岁(aOR 2.88 (95% CI 1.10-7.52), (ii)教育水平包括中等教育(aOR 2.46 (95% CI 1.43-4.24))和高等教育(aOR 3.80 (95% CI 1.54-9.37)),以及(iii)就业状况:个体农场主(aOR 6.54 (95% CI 4.36-9.80))相关。结论:我们的研究结果表明,ASFR附近的小农对T&T的了解有限。据设想,旨在培训农民的努力将弥合这一知识差距,提高认识和疾病控制策略,从而有助于预防和控制T&T。
{"title":"Farmers' knowledge, perceptions, and practices on animal trypanosomosis and the tsetse fly vector: A cross-sectional study around Kenya's Arabuko-Sokoke Forest Reserve at the livestock-wildlife interface.","authors":"Erick K Serem,&nbsp;Joel L Bargul,&nbsp;Moses M Ngari,&nbsp;Osman A Abdullahi,&nbsp;David M Mburu","doi":"10.12688/openresafrica.13397.1","DOIUrl":"https://doi.org/10.12688/openresafrica.13397.1","url":null,"abstract":"<p><p><b>Background:</b> Animal African trypanosomosis (AAT) is a veterinary disease caused by trypanosomes transmitted cyclically by tsetse flies. AAT causes huge agricultural losses in sub-Saharan Africa. Both tsetse flies and trypanosomosis (T&T) are endemic in the study area inhabited by smallholder livestock farmers at the livestock-wildlife interface around Arabuko-Sokoke Forest Reserve (ASFR) in Kilifi County on the Kenyan coast. We assessed farmers' knowledge, perceptions and control practices towards T&T. <b>Methods:</b> A cross-sectional study was conducted during November and December 2017 to collect data from 404 randomly selected cattle-rearing households using a structured questionnaire. Descriptive statistics were used to determine farmers' knowledge, perceptions, and control practices towards T&T. Demographic factors associated with knowledge of T&T were assessed using a logistic regression model. <b>Results:</b> Participants consisted of 53% female, 77% married, 30% elderly (>55 years), and the majority (81%) had attained primary education or below. Most small-scale farmers (98%) knew the tsetse fly by its local name, and 76% could describe the morphology of the adult tsetse fly by size in comparison to the housefly's ( <i>Musca domestica</i>). Only 16% of the farmers knew tsetse flies as vectors of livestock diseases. Higher chances of adequate knowledge on T&T were associated with the participants' (i) age of 15-24 years (aOR 2.88 (95% CI 1.10-7.52), (ii) level of education including secondary (aOR 2.46 (95% CI 1.43-4.24)) and tertiary (aOR 3.80 (95% CI 1.54-9.37)), and (iii) employment status: self-employed farmers (aOR 6.54 (95% CI 4.36-9.80)). <b>Conclusions:</b> Our findings suggest that small-scale farmers around ASFR have limited knowledge of T&T. It is envisaged that efforts geared towards training of the farmers would bridge this knowledge gap and sharpen the perceptions and disease control tactics to contribute to the prevention and control of T&T.</p>","PeriodicalId":74358,"journal":{"name":"Open research Africa","volume":"5 ","pages":"22"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10439355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10424253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Barriers and facilitators to maternal death surveillance and response at a busy urban National Referral Hospital in Uganda. 乌干达一个繁忙的城市国家转诊医院产妇死亡监测和应对的障碍和促进因素。
Pub Date : 2022-01-01 DOI: 10.12688/openresafrica.13438.2
Imelda Namagembe, Jolly Beyeza-Kashesya, Joseph Rujumba, Dan K Kaye, Moses Mukuru, Noah Kiwanuka, Ashley Moffett, Annettee Nakimuli, Josaphat Byamugisha

Background: Preventable maternal and newborn deaths remain a global concern, particularly in low- and- middle-income countries (LMICs) Timely maternal death surveillance and response (MDSR) is a recommended strategy to account for such deaths through identifying contextual factors that contributed to the deaths to inform recommendations to implement in order to reduce future deaths. Implementation of MDSR is still suboptimal due to barriers such as inadequate skills and leadership to support MDSR. With the leadership of WHO and UNFPA, there is momentum to roll out MDSR, however, the barriers and enablers for implementation have received limited attention. These have  implications for successful implementation. The aim of this study was: To assess barriers and facilitators to implementation of MDSR at a busy urban National Referral Hospital as perceived by health workers, administrators, and other partners in Reproductive Health. Methods: Qualitative study using in-depth interviews (24), 4 focus-group discussions with health workers, 15 key-informant interviews with health sector managers and implementing partners in Reproductive-Health. We conducted thematic analysis drawing on the Theory of Planned Behaviour (TPB).   Results: The major barriers to implementation of MDSR were: inadequate knowledge and skills; fear of blame / litigation; failure to implement recommendations; burn out because of workload   and inadequate leadership- to support health workers. Major facilitators were involving all health workers in the MDSR process, eliminate blame, strengthen leadership, implement recommendations from MDSR and functionalize lower health facilities (especially Health Centre -IVs). Conclusions: The barriers of MDSR include knowledge and skills gaps, fear of blame and litigation, and other health system factors such as erratic emergency supplies, and leadership/governance challenges. Recommendation: Efforts to strengthen MDSR for impact should use health system responsiveness approach to address the barriers identified, constructive participation of health workers to harness the facilitators and addressing the required legal framework.

背景:可预防的孕产妇和新生儿死亡仍然是一个全球关注的问题,特别是在低收入和中等收入国家(LMICs)。及时进行孕产妇死亡监测和应对(MDSR)是一项建议战略,通过确定导致死亡的背景因素来解释此类死亡,并为实施建议提供信息,以减少未来的死亡。由于支持MDSR的技能和领导不足等障碍,MDSR的实施仍然不够理想。在世卫组织和人口基金的领导下,有了推出千年发展战略的势头,然而,实施的障碍和推动因素受到的关注有限。这些都对成功实施有影响。本研究的目的是:评估卫生工作者、管理人员和生殖健康领域的其他合作伙伴在繁忙的城市国家转诊医院实施MDSR的障碍和促进因素。方法:定性研究采用深度访谈(24),与卫生工作者进行4次焦点小组讨论,与卫生部门管理人员和生殖健康实施伙伴进行15次关键信息提供者访谈。我们利用计划行为理论(TPB)进行专题分析。结果:实施MDSR的主要障碍是:知识和技能不足;害怕指责/诉讼;不执行建议;由于工作量和领导不足而筋疲力尽-以支持卫生工作者。主要的促进因素是让所有卫生工作者参与MDSR进程,消除指责,加强领导,实施MDSR的建议,并使低级卫生设施(特别是卫生中心- iv)发挥作用。结论:MDSR的障碍包括知识和技能差距,对指责和诉讼的恐惧,以及其他卫生系统因素,如应急供应不稳定,以及领导/治理挑战。建议:加强千年发展战略影响的努力应采用卫生系统响应方法来解决已确定的障碍,卫生工作者建设性参与以利用促进者并解决所需的法律框架。
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引用次数: 0
No association between fertility desire and HIV infections among men and women: Findings from community-based studies before and after implementation of an early antiretroviral therapy (ART) initiation program in the rural district of North-western Tanzania. 在坦桑尼亚西北部农村地区实施早期抗逆转录病毒治疗(ART)启动计划前后,基于社区的研究结果表明,男性和女性的生育意愿与艾滋病毒感染之间没有关联。
Pub Date : 2022-01-01 DOI: 10.12688/openresafrica.13432.1
Denna Mkwashapi, Jim Todd, Michael Mahande, John Changalucha, Mark Urassa, Milly Marston, Jenny Renju

Background: Fertility is associated with the desire to have children. The impacts of HIV and antiretroviral therapy (ART) on fertility are well known, but their impacts on the desire for children are less well known in Tanzania. We used data from two studies carried out at different periods of ART coverage in rural Tanzania to explore the relationship between HIV infection and fertility desires in men and women. Methods: We conducted secondary data analysis of the two community-based studies conducted in 2012 and 2017 in the Magu Health and Demographic system site, in Tanzania. Information on fertility desires, HIV status, and social-economic and demographic variables were analyzed. Fertility desire was defined as whether or not the participant wanted to bear a child in the next two years. The main analysis used log-binomial regression to assess the association between fertility desire and HIV infection. Results: In the 2012 study, 43% (95% CI 40.7-45.3) of men and 33.3% (95% CI 31.8 - 35.0) women wanted another child in the next two years. In 2017 the percentage rose to 55.7% (95% CI 53.6 - 57.8) in men and 41.5% (95% CI 39.8 - 43.1) in women. Although fertility desire in men and women were higher in HIV uninfected compared to HIV infected, age-adjusted analysis did not show a statistical significance difference in both studies (2012: PR=1.02, 95%CI 0.835 - 1.174, p<0.915 and 2017: PR = 0.90 95%CI 0.743 - 1.084 p= 0.262). Discussion: One-third of women and forty percent of men desired for fertility in 2012, while forty percent of women and nearly half of men desired for fertility in 2017. The data showed fertility desire, in 2012 and 2017 were not related to HIV infection in both periods of ART coverage.

背景:生育能力与想要孩子的愿望有关。艾滋病毒和抗逆转录病毒疗法(ART)对生育的影响是众所周知的,但在坦桑尼亚,它们对生育愿望的影响却鲜为人知。我们使用了两项研究的数据,这些研究是在坦桑尼亚农村地区抗逆转录病毒治疗覆盖的不同时期进行的,目的是探索艾滋病毒感染与男性和女性生育意愿之间的关系。方法:我们对2012年和2017年在坦桑尼亚Magu卫生和人口统计系统站点进行的两项基于社区的研究进行了二次数据分析。对生育意愿、艾滋病毒状况、社会经济和人口变量等信息进行了分析。生育意愿被定义为参与者是否想在未来两年内生育一个孩子。主要分析采用对数二项回归评估生育意愿与HIV感染之间的关系。结果:在2012年的研究中,43% (95% CI 40.7-45.3)的男性和33.3% (95% CI 31.8 - 35.0)的女性希望在未来两年内再要一个孩子。2017年,这一比例在男性中升至55.7% (95% CI 53.6 - 57.8),在女性中升至41.5% (95% CI 39.8 - 43.1)。尽管未感染艾滋病毒的男性和女性的生育愿望比感染艾滋病毒的更高,但年龄调整分析并未显示两项研究的统计学差异(2012年:PR=1.02, 95%CI 0.835 - 1.174, p讨论:2012年有三分之一的女性和40%的男性希望生育,而2017年有40%的女性和近一半的男性希望生育。数据显示,在抗逆转录病毒治疗覆盖的两个时期,2012年和2017年的生育意愿与艾滋病毒感染无关。
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引用次数: 1
Molecular detection of novel Anaplasma sp . and zoonotic hemopathogens in livestock and their hematophagous biting keds (genus Hippobosca) from Laisamis, northern Kenya. 新型无形体的分子检测。以及来自肯尼亚北部Laisamis的牲畜及其吸血山羊(Hippobosca属)中的人畜共患血液病。
Pub Date : 2022-01-01 DOI: 10.12688/openresafrica.13404.1
Daniel M Mwaki, Kevin O Kidambasi, Johnson Kinyua, Kenneth Ogila, Collins Kigen, Dennis Getange, Jandouwe Villinger, Daniel K Masiga, Mark Carrington, Joel L Bargul

Background: Livestock are key sources of livelihood among pastoral communities. Livestock productivity is chiefly constrained by pests and diseases. Due to inadequate disease surveillance in northern Kenya, little is known about pathogens circulating within livestock and the role of livestock-associated biting keds (genus Hippobosca) in disease transmission. We aimed to identify the prevalence of selected hemopathogens in livestock and their associated blood-feeding keds. Methods: We randomly collected 389 blood samples from goats (245), sheep (108), and donkeys (36), as well as 235 keds from both goats and sheep (116), donkeys (11), and dogs (108) in Laisamis, Marsabit County, northern Kenya. We screened all samples for selected hemopathogens by high-resolution melting (HRM) analysis and sequencing of PCR products amplified using primers specific to the genera: Anaplasma, Trypanosoma, Clostridium, Ehrlichia, Brucella, Theileria, and Babesia. Results: In goats, we detected Anaplasma ovis (84.5%), a novel Anaplasma sp. (11.8%), Trypanosoma vivax (7.3%), Ehrlichia canis (66.1%), and Theileria ovis (0.8%). We also detected A. ovis (93.5%), E. canis (22.2%), and T. ovis (38.9%) in sheep. In donkeys, we detected ' Candidatus Anaplasma camelii' (11.1%), T. vivax (22.2%), E. canis (25%), and Theileria equi (13.9%). In addition, keds carried the following pathogens; goat/sheep keds - T. vivax (29.3%) , Trypanosoma evansi (0.86%), Trypanosoma godfreyi (0.86%), and E. canis (51.7%); donkey keds - T. vivax (18.2%) and E. canis (63.6%); and dog keds - T. vivax (15.7%), T. evansi (0.9%), Trypanosoma simiae (0.9%) , E. canis (76%), Clostridium perfringens (46.3%), Bartonella schoenbuchensis (76%), and Brucella abortus (5.6%). Conclusions: We found that livestock and their associated ectoparasitic biting keds carry a number of infectious hemopathogens, including the zoonotic B. abortus. Dog keds harbored the most pathogens, suggesting dogs, which closely interact with livestock and humans, as key reservoirs of diseases in Laisamis. These findings can guide policy makers in disease control.

背景:牲畜是牧区的主要生计来源。牲畜生产力主要受到病虫害的制约。由于肯尼亚北部的疾病监测不足,人们对牲畜中传播的病原体以及与牲畜相关的咬头犬(河马属)在疾病传播中的作用知之甚少。我们的目的是确定选定的血液病原体在家畜及其相关的血液喂养方式中的流行程度。方法:在肯尼亚北部Marsabit县Laisamis随机采集山羊(245只)、绵羊(108只)和驴(36只)血样389份,山羊和绵羊(116只)、驴(11只)和狗(108只)血样235份。我们通过高分辨率熔融(HRM)分析和PCR扩增产物测序对所有样本进行筛选,这些扩增产物使用特异性引物扩增:无形体、锥虫、梭状芽胞杆菌、埃利希氏体、布鲁氏菌、希氏菌和巴贝斯虫。结果:在山羊中检出羊无原体(84.5%)、新型无原体(11.8%)、间日锥虫(7.3%)、犬埃利希体(66.1%)和山羊伊氏杆菌(0.8%)。绵羊中分别检出了93.5%、22.2%和38.9%的羊单胞杆菌、犬单胞杆菌。在驴中,我们检出了“山驼峰候选菌”(11.1%)、间日疟原虫(22.2%)、犬伊氏杆菌(25%)和马伊氏杆菌(13.9%)。此外,keds还携带以下病原体;山羊/绵羊:间日绦虫(29.3%)、伊文氏锥虫(0.86%)、哥德弗雷锥虫(0.86%)和犬伊氏锥虫(51.7%);驴-间日疟(18.2%)和犬伊蚊(63.6%);犬类:间日疟(15.7%)、伊文氏体(0.9%)、类人锥虫(0.9%)、犬伊氏体(76%)、产气荚膜梭菌(46.3%)、舍恩布氏巴尔通体(76%)、流产布鲁氏菌(5.6%)。结论:我们发现家畜及其相关的体外寄生虫叮咬儿童携带多种传染性血液病,包括人畜共患病的abortus b。狗的粪便中含有最多的病原体,这表明与牲畜和人类密切互动的狗是Laisamis疾病的主要宿主。这些发现可以指导决策者进行疾病控制。
{"title":"Molecular detection of novel <i>Anaplasma</i> sp <i>.</i> and zoonotic hemopathogens in livestock and their hematophagous biting keds (genus <i>Hippobosca</i>) from Laisamis, northern Kenya.","authors":"Daniel M Mwaki,&nbsp;Kevin O Kidambasi,&nbsp;Johnson Kinyua,&nbsp;Kenneth Ogila,&nbsp;Collins Kigen,&nbsp;Dennis Getange,&nbsp;Jandouwe Villinger,&nbsp;Daniel K Masiga,&nbsp;Mark Carrington,&nbsp;Joel L Bargul","doi":"10.12688/openresafrica.13404.1","DOIUrl":"https://doi.org/10.12688/openresafrica.13404.1","url":null,"abstract":"<p><p><b>Background:</b> Livestock are key sources of livelihood among pastoral communities. Livestock productivity is chiefly constrained by pests and diseases. Due to inadequate disease surveillance in northern Kenya, little is known about pathogens circulating within livestock and the role of livestock-associated biting keds (genus <i>Hippobosca</i>) in disease transmission. We aimed to identify the prevalence of selected hemopathogens in livestock and their associated blood-feeding keds. <b>Methods:</b> We randomly collected 389 blood samples from goats (245), sheep (108), and donkeys (36), as well as 235 keds from both goats and sheep (116), donkeys (11), and dogs (108) in Laisamis, Marsabit County, northern Kenya. We screened all samples for selected hemopathogens by high-resolution melting (HRM) analysis and sequencing of PCR products amplified using primers specific to the genera: <i>Anaplasma, Trypanosoma, Clostridium, Ehrlichia, Brucella, Theileria,</i> and <i>Babesia.</i> <b>Results:</b> In goats, we detected <i>Anaplasma ovis</i> (84.5%), a novel <i>Anaplasma</i> sp. (11.8%), <i>Trypanosoma vivax</i> (7.3%), <i>Ehrlichia canis</i> (66.1%), and <i>Theileria ovis</i> (0.8%). We also detected <i>A. ovis</i> (93.5%), <i>E. canis</i> (22.2%), and <i>T. ovis</i> (38.9%) in sheep. In donkeys, we detected ' <i>Candidatus</i> Anaplasma camelii' (11.1%), <i>T. vivax</i> (22.2%), <i>E. canis</i> (25%), and <i>Theileria equi</i> (13.9%). In addition, keds carried the following pathogens; goat/sheep keds - <i>T. vivax</i> (29.3%) <i>, Trypanosoma evansi</i> (0.86%), <i>Trypanosoma godfreyi</i> (0.86%), and <i>E. canis</i> (51.7%); donkey keds - <i>T. vivax</i> (18.2%) and <i>E. canis</i> (63.6%); and dog keds - <i>T. vivax</i> (15.7%), <i>T. evansi</i> (0.9%), <i>Trypanosoma simiae</i> (0.9%) <i>,</i> <i>E. canis</i> (76%), <i>Clostridium perfringens</i> (46.3%), <i>Bartonella</i> <i>schoenbuchensis</i> (76%), and <i>Brucella abortus</i> (5.6%). <b>Conclusions:</b> We found that livestock and their associated ectoparasitic biting keds carry a number of infectious hemopathogens, including the zoonotic <i>B. abortus</i>. Dog keds harbored the most pathogens, suggesting dogs, which closely interact with livestock and humans, as key reservoirs of diseases in Laisamis. These findings can guide policy makers in disease control.</p>","PeriodicalId":74358,"journal":{"name":"Open research Africa","volume":"5 ","pages":"23"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10314185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9791675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preferences and Expectations of Feedback of Individual Genetic Research Results in African Genomics: Views of South African Parents of Children With Neurodevelopmental Conditions 非洲基因组学个体遗传学研究结果反馈的偏好和期望:患有神经发育疾病儿童的南非父母的观点
Pub Date : 2020-11-23 DOI: 10.21203/rs.3.rs-107280/v1
O. Matshabane, Cleo A Albertus, Marlyn C. Faure, D. Ralefala, K. Donald, A. Wonkam, J. D. Vries
BackgroundGenomic medicine is expanding at an exponential pace across the globe and increased access to genome analysis has led to greater generations of genetic results with specific relevance to individuals. AimThis study aims to explore preferences and expectations of feedback of individual genetic research results among parents of children with neurodevelopmental conditions. MethodsFollowing a qualitative approach, we conducted four deliberative focus group discussions with (n=27) South African parents of children involved in genomics research on neurodevelopmental conditions. ResultsMost participants expressed a strong interest in receiving individual genetic results regardless of severity, actionability and preventability. These results were viewed as valuable because they could empower or emancipate individuals, families and communities. Receiving risk information was also believed to motivate healthier lifestyle choices. However, some participants were uncertain or articulated a desire not to receive results due to fears of anxiety or psychological harm. In addition, participants expected to receive results as a demonstration of respect from researchers and articulated it as an act to build trust between researchers and participants. ConclusionsInternationally, a debate continues around whether individual genetic results should or should not be fed back to participants of research studies. In Africa, there is scant literature which has investigated this question and no policies to guide researchers. This study provides a basis of empirical data on perspectives of African participants which could inform work on the development of a consolidated approach to the feedback of incidental findings in the continent.
基因组医学正在全球范围内以指数级的速度扩张,基因组分析的增加导致了更一代与个体特定相关的遗传结果。目的探讨神经发育障碍患儿家长对个体基因研究结果反馈的偏好和期望。方法采用定性方法,我们与参与神经发育疾病基因组学研究的儿童的南非父母(n=27)进行了四次审议焦点小组讨论。大多数参与者表达了对接受个体遗传结果的强烈兴趣,而不管其严重性、可操作性和可预防性。这些结果被认为是有价值的,因为它们可以使个人、家庭和社区获得权力或解放。接受风险信息也被认为能促使人们选择更健康的生活方式。然而,由于担心焦虑或心理伤害,一些参与者不确定或明确表示不希望收到结果。此外,参与者期望得到研究人员尊重的结果,并将其表述为在研究人员和参与者之间建立信任的行为。在国际上,关于个体基因结果是否应该反馈给研究参与者的争论仍在继续。在非洲,调查这个问题的文献很少,也没有指导研究人员的政策。这项研究提供了关于非洲参与者观点的经验数据的基础,可以为制定一种综合方法来反馈非洲大陆偶然发现的工作提供信息。
{"title":"Preferences and Expectations of Feedback of Individual Genetic Research Results in African Genomics: Views of South African Parents of Children With Neurodevelopmental Conditions","authors":"O. Matshabane, Cleo A Albertus, Marlyn C. Faure, D. Ralefala, K. Donald, A. Wonkam, J. D. Vries","doi":"10.21203/rs.3.rs-107280/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-107280/v1","url":null,"abstract":"\u0000 BackgroundGenomic medicine is expanding at an exponential pace across the globe and increased access to genome analysis has led to greater generations of genetic results with specific relevance to individuals. AimThis study aims to explore preferences and expectations of feedback of individual genetic research results among parents of children with neurodevelopmental conditions. MethodsFollowing a qualitative approach, we conducted four deliberative focus group discussions with (n=27) South African parents of children involved in genomics research on neurodevelopmental conditions. ResultsMost participants expressed a strong interest in receiving individual genetic results regardless of severity, actionability and preventability. These results were viewed as valuable because they could empower or emancipate individuals, families and communities. Receiving risk information was also believed to motivate healthier lifestyle choices. However, some participants were uncertain or articulated a desire not to receive results due to fears of anxiety or psychological harm. In addition, participants expected to receive results as a demonstration of respect from researchers and articulated it as an act to build trust between researchers and participants. ConclusionsInternationally, a debate continues around whether individual genetic results should or should not be fed back to participants of research studies. In Africa, there is scant literature which has investigated this question and no policies to guide researchers. This study provides a basis of empirical data on perspectives of African participants which could inform work on the development of a consolidated approach to the feedback of incidental findings in the continent.","PeriodicalId":74358,"journal":{"name":"Open research Africa","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41565545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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Open research Africa
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