Nerve damage is a major complication of surgery, causing pain and loss of function. We have identified novel near-infrared nerve-specific fluorophores that provide excellent nerve contrast with the ability to identify buried nerve tissue.
Nerve damage is a major complication of surgery, causing pain and loss of function. We have identified novel near-infrared nerve-specific fluorophores that provide excellent nerve contrast with the ability to identify buried nerve tissue.
A fast time-gated phasor analysis tool was developed and used to monitor kinetics of NIR FLIM-FRET in vitro and in vivo. The results were validated by comparison with standard two-component fluorescence decay fitting analysis.
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