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Compost Application to Degraded Vineyard Soils: Effect on Soil Chemistry, Fertility, and Vine Performance 在退化的葡萄园土壤上施用堆肥:对土壤化学、肥力和葡萄生长性能的影响
IF 1.9 3区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2020-10-09 DOI: 10.5344/ajev.2020.20012
Stewart Wilson, J. Lambert, R. Dahlgren
A two-year experiment investigated the effects of compost application rate on soil chemical properties, vine nutrient status, vine performance, and grape juice characteristics in a degraded vineyard soil in northern California. The intent of the research was to identify vineyard management strategies to improve soil fertility and to identify optimal compost application rates. We applied composted steer manure at three rates (11.2, 22.4, and 33.6 t/ha) in a randomized complete block design before the 2012 growing season. Pruning and berry weight increased over the control at the highest application rate in both years, while vine yield significantly increased over the control in year two. Polynomial orthogonal contrasts suggest that pruning weight, vine yield, and berry weight increased linearly with increasing compost application rate in 2012, and that vine yield and berry weight increased linearly and quadratically with compost application rate in 2013. Measured soil properties increased from compost application, including nitrogen (N), carbon, pH, exchangeable potassium (K), manganese, and calcium and available phosphorus (P, Olsen-P), while phosphorus fixation decreased. Vine petiole nutrients (N, P, and K) significantly increased from compost application in both years. Juice characteristics (pH, total soluble solids, and titratable acidity) were unaffected by compost application. Similarly, vine balance was unaffected by compost application. All vine metrics improved at the highest application rate, and soil chemical properties increased with the two highest application rates. Therefore, significant benefits to soil fertility and vine performance can be achieved for at least two years in degraded vineyard soils following a single dose of compost at higher application rates (22.4 and 33.6 t/ha) without compromising juice characteristics or vine balance.
在加州北部一个退化的葡萄园土壤中,研究了堆肥施用量对土壤化学性质、葡萄藤营养状况、葡萄藤生产性能和葡萄汁特性的影响。本研究的目的是确定葡萄园管理策略,以提高土壤肥力,并确定最佳堆肥施用量。在2012年生长季节之前,我们采用随机完全区组设计,以三种比例(11.2、22.4和33.6吨/公顷)施用堆肥牛粪。在最高施用量下,两年的修剪量和果实重量均比对照增加,而第二年的葡萄产量显著高于对照。多项式正交对比表明,2012年随堆肥用量的增加,整枝重、葡萄产量和浆果重呈线性增长,2013年随堆肥用量的增加,葡萄产量和浆果重呈线性和二次增长。施用堆肥增加了土壤的氮、碳、pH、交换性钾、锰、钙和有效磷(P、Olsen-P),而固磷减少。两年内施用堆肥显著增加了藤叶柄养分(N、P、K)。果汁特性(pH,总可溶性固形物和可滴定酸度)不受堆肥施用的影响。同样,藤的平衡不受堆肥施用的影响。在最高施用量下,植株各项指标均有改善,土壤化学性质随两个最高施用量的增加而增加。因此,在退化的葡萄园土壤中,以较高的施用量(22.4和33.6吨/公顷)施用单剂量堆肥,在不影响果汁特性或葡萄藤平衡的情况下,至少两年内可以实现土壤肥力和葡萄藤性能的显著提高。
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引用次数: 7
Influence of Select Non-Saccharomyces Yeast on Hanseniaspora uvarum Growth during Prefermentation Cold Maceration 选择性非酵母菌在冷浸过程中对羊粪菌生长的影响
IF 1.9 3区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2020-10-01 DOI: 10.5344/ajev.2020.20004
Jared Johnson, Mengying Fu, M. Qian, C. Curtin, J. Osborne
This study investigated the effect of select non-Saccharomyces yeast strains on Hanseniaspora uvarum growth and acetic acid and ethyl acetate production during prefermentation cold soak. We tested commercially available non-Saccharomyces yeasts for their ability to reduce H. uvarum growth and acetic acid production during a simulated cold soak in a grape juice-based medium. All tested non-Saccharomyces yeast reduced H. uvarum growth and acetic acid production, with some yeast having a greater impact than others. Following the screening of non-Saccharomyces yeast, we tested 14 different H. uvarum isolates against a selected non-Saccharomyces yeast, Metschnikowia fructicola, and found that all H. uvarum isolates had reduced growth and acetic acid production when grown in co-culture with M. fructicola, with variation between isolates noted. Finally, we evaluated the effect of M. fructicola on H. uvarum during prefermentation cold soak of Pinot noir grapes. Pinot noir grapes were inoculated with a combination of H. uvarum and M. fructicola and cold soaked for six days at 8°C. At the end of cold soaking, treatments inoculated with M. fructicola contained lower populations of H. uvarum and significantly lower acetic acid and ethyl acetate concentrations compared with treatments not inoculated with M. fructicola. After the completion of alcoholic fermentation, wines where M. fructicola was added contained significantly lower ethyl acetate but no differences in acetic acid concentration. These results suggest that adding select non-Saccharomyces yeast may be another method to reduce the risk of spoilage by H. uvarum during prefermentation cold soaking.
本试验研究了选定的非酵母菌菌株在冷浸发酵过程中对鹿茸菌生长及乙酸和乙酸乙酯产量的影响。我们测试了市售的非酵母菌酵母菌在葡萄汁培养基中模拟冷浸过程中减少吸血弧菌生长和醋酸产生的能力。所有测试的非酵母菌酵母菌都减少了吸血嗜血杆菌的生长和醋酸的产生,其中一些酵母菌的影响比其他酵母菌更大。在对非酵母菌进行筛选之后,我们对14种不同的uvarum分离株与选定的非酵母菌——果霉Metschnikowia fructicola进行了测试,发现所有的uvarum分离株与果霉m.s ucticola共培养时,生长和乙酸产量都有所下降,并注意到不同分离株之间的差异。最后,我们评价了在黑皮诺葡萄优先冷浸过程中,果霉菌对uvarum菌的影响。用uvarum和m.s ucticola组合接种黑皮诺葡萄,在8°C下冷浸6天。在冷浸泡结束时,接种果霉处理的uvarum种群数量和乙酸、乙酸乙酯浓度显著低于未接种果霉处理。在酒精发酵完成后,加入果霉的葡萄酒中乙酸乙酯含量显著降低,但乙酸浓度没有差异。这些结果表明,添加精选的非酵母菌可能是另一种方法,以减少在优选冷浸泡期间被uvarum变质的风险。
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引用次数: 8
Efficacy of Warmed Wine Against Brettanomyces bruxellensis Present in Oak Barrel Staves 温热葡萄酒对橡木桶壁布氏酵母的抑菌作用
IF 1.9 3区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2020-10-01 DOI: 10.5344/ajev.2020.19082
Z. Cartwright, C. G. Edwards
New barrels (16 L) differing by oak species (Quercus alba or Q. petraea) and toasting level (light or heavy) were infected with Brettanomyces bruxellensis. Infected staves were sawed into 3 cm × 3 cm cubes and immersed 2 mm into red wine (11 or 15% v/v alcohol) that had been heated to 35, 40, 45, or 50°C. After removal from the wine, cubes were either sawed into cross sections or prepared as oak shavings before transfer to a yeast recovery medium and incubated for ≥30 days (cross sections) or for 12 hr (shavings) to recover culturable populations and calculate decimal reduction times (DT-values). Culturable cells were not recovered from inner cross sectional layers (0 to 4 mm depth) after heating in 11% v/v alcohol wine at 45 or 50°C, whereas populations were destroyed at deeper depths (e.g., 5 to 9 mm) using wines of 15% v/v alcohol at these same temperatures. In agreement, DT-values were greater when cubes were heated in 11% v/v alcohol wines (D45°C = 46 sec, D50°C = 30 sec) compared to wines with 15% v/v alcohol (D45°C = 17 sec, D50°C = 9 sec). Compared to heated water or steam, warmed-wine treatments required lower temperatures to remove the same degree of microbial contamination, in particular at inner stave depths ≤4 mm. Similar observations were noted for commercial barrels (225 L) previously infected by unidentified (in-house) strains of B. bruxellensis. Thus, application of warmed wine to infected barrels may serve as a method to greatly reduce populations of B. bruxellensis when temperatures lower than those needed for hot water or steam treatment are desired.
不同橡木品种(白栎或白栎)和烘烤水平(轻或重)的新桶(16L)感染了布鲁谢布列塔尼酵母。将感染的棒锯成3厘米×3厘米的立方体,并将2毫米浸入加热至35、40、45或50°C的红酒(11或15%v/v酒精)中。从葡萄酒中取出后,在转移到酵母回收培养基中之前,将立方体锯成横截面或制备为橡木屑,并孵育≥30天(横截面)或12小时(刨花),以回收可培养种群并计算十进制减少时间(DT值)。在45或50°C的11%v/v酒精葡萄酒中加热后,无法从内部横截面层(0至4 mm深度)中回收可培养细胞,而在这些相同温度下,使用15%v/v酒精的葡萄酒在更深的深度(例如5至9 mm)中破坏种群。一致的是,与含15%v/v酒精的葡萄酒(D45°C=17秒,D50°C=9秒)相比,在11%v/v酒精葡萄酒中加热立方体时(D45℃=46秒,D55℃=30秒),DT值更大。与热水或蒸汽相比,加热葡萄酒处理需要更低的温度来去除相同程度的微生物污染,特别是在内部冷却壁深度≤4毫米的情况下。在商业桶(225升)中也观察到了类似的观察结果,这些桶之前曾感染过未经鉴定的(内部)布鲁氏芽孢杆菌菌株。因此,当需要低于热水或蒸汽处理所需温度的温度时,将温热的葡萄酒应用于受感染的酒桶可以作为一种大大减少布鲁氏芽孢杆菌数量的方法。
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引用次数: 0
Free, Bound, and Total Sulfur Dioxide (SO2) during Oxidation of Wines 葡萄酒氧化过程中的游离、结合和总二氧化硫(SO2)
IF 1.9 3区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2020-10-01 DOI: 10.5344/ajev.2020.19083
G. Sacks, Patricia A. Howe, M. Standing, J. Danilewicz
Recent work has evaluated the kinetics of free and total sulfur dioxide (SO2) loss in wine following aerial exposure, but little work exists on the relationship between free and total SO2 during the oxidation conditions expected during wine storage. We report changes in free and total SO2 in three wines (Chardonnay, Merlot, and Cabernet Sauvignon) stored for up to 400 days at 19 or 31°C in different bag-in-box packages. The rate of total SO2 loss varied up to seven-fold for a given wine across storage conditions, e.g., 0.13 to 0.94 mg/L SO2 loss per day for the Chardonnay. Total rates of SO2 loss were linear even after free SO2 was undetectable, and only strongly bound SO2 remained. This finding indicated that bound SO2 adduct hydrolysis was fast, as compared to the rate of SO2 consumption in these packaged wines, likely because the SO2 consumption rate depended on the oxygen ingress rate. Total versus free SO2 plots were linear for free SO2 concentrations greater than ~5 mg/L. The slope (Δtotal/Δfree) of this linear region was wine dependent (Chardonnay = 1.33 to 1.49; Merlot = 1.91 to 2.10; Cabernet Sauvignon = 1.83 to 2.00). In contrast to the rate of total SO2 loss, this ratio varied negligibly with storage conditions, suggesting that minimal formation of new SO2 binders occurred over the course of oxidation. The apparent adduct dissociation equilibrium constant (Kd) of each wine as a function of free SO2 concentration was determined from the first derivative of [Bound]/[Free] versus [Free] plots (“Burroughs plots”). As free SO2 decreased to <10 mg/L, the apparent Kd values decreased to values between 1 × 10-4 M and 10-5 M, which are comparable to those reported for malodorous aldehydes.
最近的工作评估了空气暴露后葡萄酒中游离二氧化硫和总二氧化硫(SO2)损失的动力学,但在葡萄酒储存过程中预期的氧化条件下,游离二氧化硫与总二氧化硫之间的关系研究很少。我们报告了三种葡萄酒(霞多丽、梅洛和赤霞珠)在不同的袋装包装中,在19或31°C下储存长达400天,其游离SO2和总SO2的变化。在不同的储存条件下,给定葡萄酒的总SO2损失率变化高达7倍,例如霞多丽每天损失0.13至0.94 mg/L的SO2。即使在检测不到游离SO2之后,SO2的总损失率也是线性的,并且只剩下强烈结合的SO2。这一发现表明,与这些包装葡萄酒中的SO2消耗速率相比,结合的SO2加合物水解速度很快,可能是因为SO2消耗速率取决于氧气进入速率。游离SO2浓度大于~5 mg/L时,总SO2与游离SO2的关系图呈线性。该线性区域的斜率(Δtotal/Δfree)取决于葡萄酒(霞多丽=1.33至1.49;梅洛=1.91至2.10;赤霞珠=1.83至2.00)。与总SO2损失率相比,该比率随储存条件的变化可忽略不计,这表明在氧化过程中新SO2结合物的形成最小。每种葡萄酒的表观加合物离解平衡常数(Kd)作为游离SO2浓度的函数,由[结合]/[游离]与[游离]图(“Burroughs图”)的一阶导数确定。当游离SO2降至<10mg/L时,表观Kd值降至1×10-4M至10-5M之间,与恶臭醛的报告值相当。
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引用次数: 10
Evaluation of Ripening Indicators for Harvest-time Decision Making in Cold Climate Grape Production 寒冷气候葡萄生产成熟度指标的评价
IF 1.9 3区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2020-10-01 DOI: 10.5344/ajev.2020.20022
J. Scharfetter, Andi Nelson, B. Workmaster, A. Atucha
Currently, growers and winemakers of cold climate interspecific hybrid grape (CCIHG) cultivars rely solely on the technological maturity variables Brix, titratable acidity (TA), and pH as ripening indicators for harvest and winemaking decisions. In contrast, using additional chemical variables to determine optimal harvest times is a common practice in most regions of the world where the European grape Vitis vinifera is cultivated. As there are significant differences in berry chemistry between CCIHG and V. vinifera, the objective of the present study was to evaluate, by linear regression coupled with principal component analysis and cluster analysis, the suitability of six additional maturity variables (berry fresh weight, total protein, total phenolics, monomeric anthocyanins, percent polymeric color, and total tannins) as potential ripening indicators for the CCIHG cultivars Brianna, Frontenac, La Crescent, Léon Millot, Maréchal Foch, Marquette, MN 1220, and Petite Pearl. We observed that berry fresh weight, total phenolics, and percent polymeric color had inconsistent and difficult-to-model relationships from year to year and across distinct grape cluster microclimate treatments, making them unsuitable as ripening indicators. In addition to the standard technological maturity variables of Brix, TA, and pH, the variables of total protein concentration for white cultivars and of monomeric anthocyanin, total protein, and total tannin concentrations for red cultivars have consistent linear relationships from veraison to harvest, making them good candidates for ripening indicators.
目前,寒冷气候种间杂交葡萄(CCIHG)品种的种植者和酿酒师仅依靠技术成熟度变量白利度、可滴定酸度(TA)和pH作为成熟指标来决定收成和酿酒决策。相比之下,使用额外的化学变量来确定最佳收获时间是世界上大多数种植欧洲葡萄的地区的常见做法。由于CCIHG和葡萄果实的化学成分存在显著差异,本研究的目的是通过线性回归结合主成分分析和聚类分析,评价6个额外的成熟度变量(浆果鲜重、总蛋白质、总酚类、单体花青素、聚合物颜色百分比和总单宁)作为CCIHG品种Brianna、Frontenac、La Crescent、lsamon Millot, marsamchal Foch, Marquette, mn1220和Petite Pearl。我们观察到,浆果鲜重、总酚类物质和聚合物颜色百分比在不同年份和不同葡萄簇小气候处理之间存在不一致且难以建模的关系,因此它们不适合作为成熟指标。除了白度、TA和pH等标准技术成熟度变量外,白色品种的总蛋白浓度变量以及红色品种的单体花青素、总蛋白和总单宁浓度变量从品种到收获都具有一致的线性关系,使它们成为成熟指标的良好候选者。
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引用次数: 1
Viticultural and Controlled Phenolic Release Treatments Affect Phenolic Concentration and Tannin Composition in Pinot noir Wine 葡萄栽培和控制酚释放处理对黑比诺葡萄酒中酚浓度和单宁组成的影响
IF 1.9 3区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2020-10-01 DOI: 10.5344/ajev.2020.19003
A. Carew, F. Kerslake, K. Bindon, P. Smith, D. Close, R. Dambergs
Pinot noir fruit is often relatively low in the phenolics important for wine mouthfeel and color stabilization. Previous research has shown that leaf removal can influence the concentration and composition of Pinot noir fruit phenolics, but it is not clear to what extent these effects on grape composition are carried through the winemaking process. A novel thermal treatment, Controlled Phenolic Release (CPR), has been demonstrated to be effective for increasing the phenolic concentration of Pinot noir wines, but there is currently limited information on the interaction between this process and viticultural practices. CPR is microwave heating of grape must to hasten phenolic extraction. This study applied viticultural treatments to Pinot noir (no leaf removal, leaf removal at postflowering and preveraison) in two commercial vineyards (A and B), and winemaking treatments in replicated microvinifications (control, CPR with skin contact, CPR with early pressing) were applied to fruit from vineyard A. Effects on grape and wine phenolics were examined using UV-visible spectrophotometry. Wine tannin composition and polymerization were assayed by methyl cellulose precipitation and high-performance liquid chromatography. Viticultural treatments applied produced significant effects in vineyard A but not in vineyard B. Winemaking treatments affected the concentration of phenolics in wines, but there was no interaction between viticultural and winemaking treatments; effects were additive. Of scientific and practical significance was the finding that grape phenolic concentration determined from homogenate extractions of fruit did not reliably predict Pinot noir wine phenolics. Furthermore, in wines, variation in tannin composition, the extent of tannin polymerization, and tannin size was found between treatments. The novel findings here are that the viticultural treatment and the winemaking treatments were both significant and, being independent of each other, offered two separate ways to adjust phenolics in Pinot noir wine. Viticultural and winemaking treatments were also shown to affect tannin composition, with potential mouthfeel and color stability implications.
黑皮诺果实的酚类物质含量通常相对较低,这对葡萄酒的口感和颜色稳定性很重要。先前的研究表明,落叶会影响黑比诺果实酚类物质的浓度和成分,但尚不清楚这些对葡萄成分的影响在多大程度上是通过酿酒过程产生的。一种新的热处理,控制酚释放(CPR),已被证明对提高黑皮诺葡萄酒的酚浓度有效,但目前关于这一过程与葡萄栽培实践之间的相互作用的信息有限。CPR是微波加热葡萄必须加速酚类物质的提取。本研究对两个商业葡萄园(A和B)的黑比诺进行了葡萄栽培处理(不落叶、开花后和前期落叶),并对葡萄园A的果实进行了复制微环境下的酿酒处理(对照、皮肤接触的CPR、早期压榨的CPR)。用紫外-可见分光光度法测定了对葡萄和葡萄酒酚类物质的影响。采用甲基纤维素沉淀法和高效液相色谱法测定了葡萄酒单宁的组成和聚合度。葡萄栽培处理对葡萄园A产生了显著影响,但对葡萄园B没有影响。酿酒处理影响葡萄酒中酚类物质的浓度,但葡萄栽培和酿酒处理之间没有相互作用;效果是相加的。具有科学和实际意义的是,从果实匀浆提取物中测定葡萄酚类物质的浓度并不能可靠地预测黑比诺葡萄酒的酚类物质。此外,在葡萄酒中,单宁成分、单宁聚合程度和单宁大小在不同处理之间存在差异。这里的新发现是,葡萄栽培处理和酿酒处理都很重要,并且相互独立,提供了两种不同的方法来调节黑比诺葡萄酒中的酚类物质。葡萄栽培和酿酒处理也会影响单宁的组成,并可能影响口感和颜色稳定性。
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引用次数: 2
Terroir of Winter Hardiness: Bud LT50, Water Metrics, Yield, and Berry Composition in Ontario Riesling 耐寒风土:安大略雷司令的芽LT50、水分指标、产量和浆果成分
IF 1.9 3区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2020-10-01 DOI: 10.5344/ajev.2020.19084
Mary Jasinski, A. Reynolds, F. Di Profio, Audrey C. S. Pasquier, Maxime Touffet, Rea Fellman, Hyun-Suk Lee
Grapevine winter hardiness is governed by several factors besides extreme weather conditions, such as site-specific soil factors (texture, composition, moisture, drainage), vine water status, and yield. It was hypothesized that winter hardiness would be influenced by specific vineyard terroir-driven factors and that zones in vineyards with low water status (leaf water potential [Ψ]) would likewise be more winter hardy than vines with high water status (less negative leaf Ψ). Six Riesling vineyards were chosen throughout the Niagara region in Ontario. Data were collected at fruit set, lag phase, and veraison (soil water content [SWC], leaf Ψ), at harvest (yield components, berry composition), and three times during the winter (LT50; the temperature at which 50% of buds die) in 2010 to 2012. Interpolation and mapping of variables was completed using kriging, and statistical analyses (linear correlation, k-means clustering, principal components analysis, multilinear regression) were performed. SWC, leaf Ψ, yield components, berry composition, and LT50 were spatially clustered in each vineyard. GIS and multilinear regression revealed that leaf Ψ could predict the LT50 value, with strong correlations observed between LT50 and leaf Ψ values in most of the vineyard blocks in 2010 to 2011 (4/6 and 5/6, respectively). In the extremely dry 2012 season, leaf Ψ (range across sites at veraison 0.9 to 1.4 MPa) was positively correlated to LT50, yield, titratable acidity, pH, and Brix and inversely correlated to SWC and monoterpene concentration. Results suggest that there is a spatial component to winter hardiness, as with other aspects of terroir. This study allows a method to compare winter hardiness to other critical variables to better understand terroir-based factors of the Niagara region.
葡萄的抗寒性除了受到极端天气条件的影响外,还受到几个因素的影响,如特定地点的土壤因素(质地、成分、水分、排水)、葡萄的水分状况和产量。据推测,冬季耐寒性将受到特定葡萄园风土驱动因素的影响,低水分状态(叶水势[Ψ])的葡萄园区域同样比高水分状态(负叶Ψ较少)的葡萄藤更耐寒。安大略省尼亚加拉地区共有六个雷司令葡萄园。2010年至2012年,在结实期、滞后期和veraison(土壤含水量[SWC]、叶片Ψ)、收获期(产量成分、浆果成分)和冬季三次(LT50;50%芽死亡的温度)收集数据。使用克里格法完成变量的插值和映射,并进行统计分析(线性相关、k-means聚类、主成分分析、多元线性回归)。SWC、叶片Ψ、产量组成、浆果成分和LT50在每个葡萄园中都是空间聚类的。GIS和多元线性回归表明,叶片Ψ可以预测LT50值,在2010年至2011年的大部分葡萄园区块中,LT50与叶片Ψ值之间存在很强的相关性(分别为4/6和5/6)。在2012年极度干旱的季节,叶片Ψ(在veraison 0.9至1.4MPa的位置范围内)与LT50、产量、可滴定酸度、pH和Brix呈正相关,与SWC和单萜浓度呈负相关。结果表明,与风土的其他方面一样,抗寒性也有空间成分。这项研究提供了一种将抗寒性与其他关键变量进行比较的方法,以更好地了解尼亚加拉地区基于风土的因素。
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引用次数: 2
Stilbenes Can Impair Malolactic Fermentation with Strains of Oenococcus oeni and Lactobacillus plantarum 二苯乙烯可抑制酒酒球菌和植物乳杆菌对苹果酸乳酸的发酵
IF 1.9 3区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2020-09-10 DOI: 10.5344/ajev.2020.20021
Sabrina Zimdars, Rita Caspers-Weiffenbach, Pascal Wegmann-Herr, Fabian Weber
Malolactic fermentation (MLF) is an important step in winemaking to improve wine quality by deacidification, increased microbial stability, and modified wine flavor. The phenolic composition of the wine influences the growth and metabolism of the lactic acid bacteria (LAB) used for MLF. Due to increased cultivation of fungus-resistant grape cultivars, the present study aimed to analyze the influence of stilbenes on MLF because stilbenes play an important role as phytoalexins in plants to counteract fungal pathogens. The analysis showed that the grapevine-shoot extract Vineatrol and the stilbenes ε-viniferin, ampelopsin A, r2-viniferin, and r-viniferin, as well as mixtures of the five stilbenes, decelerated malic acid degradation. This can be partially attributed to a reduced number of viable cells. However, the direct impact of stilbenes on the malolactic enzyme must also play a role, as shown by the finding that the viability of LAB stabilized, while at the same time, malic acid degradation was reduced. These results show that wines made from stilbene-rich grapes, such as fungus-resistant cultivars, might have a higher risk for a sluggish or incomplete MLF.
苹果酸乳酸发酵(MLF)是通过脱酸、提高微生物稳定性和修饰葡萄酒风味来提高葡萄酒品质的重要步骤。葡萄酒的酚类成分影响乳酸菌(LAB)用于MLF的生长和代谢。由于抗真菌葡萄品种的增加,本研究旨在分析芪对MLF的影响,因为芪在植物中发挥重要的植物抗毒素作用,以对抗真菌病原体。结果表明,葡萄芽提取物vinatrol与stilbenes ε- vinifin、ampelopsin A、r2- vinifin、r- vinifin以及这五种stilbenes的混合物均能减缓苹果酸的降解。这可以部分归因于活细胞数量的减少。然而,二苯乙烯对苹果酸乳酸酶的直接影响也一定起到了一定的作用,研究结果表明,LAB的活力稳定了,同时苹果酸的降解也降低了。这些结果表明,由富含苯乙烯的葡萄酿制的葡萄酒,如抗真菌品种,可能有更高的风险出现缓慢或不完整的MLF。
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引用次数: 2
Whole Cluster and Dried Stem Additions’ Effects on Chemical and Sensory Properties of Pinot noir Wines over Two Vintages 整簇和干茎添加对两个年份黑比诺葡萄酒化学和感官特性的影响
IF 1.9 3区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2020-08-20 DOI: 10.5344/ajev.2020.20037
L. F. Casassa, Niclas P. Dermutz, P. Mawdsley, M. Thompson, A. Catania, Thomas S. Collins, P. L. Ashmore, Fintan du Fresne, Gregory Gasic, J. D. Dodson Peterson
The effect of additions of dried stems (DS) and whole clusters at 50% (50% WC) and 100% (100% WC) were investigated over two consecutive vintages of Pinot noir wines from the Edna Valley AVA of California (USA) at commercial scale. Addition of 100% WC led to significant increases in pH and volatile acidity. Anthocyanins, polymeric pigments, and color were more influenced by vintage than by the winemaking treatments, but they were unaffected or negatively affected in 100% WC wines. Conversely, the tannin content of the wines increased in accordance to the percentage of WC and stems added, with increases of 68% (50% WC), 100% (100% WC), and 90% (DS) in 2016 and 61% (50% WC), 123% (100% WC), and 137% (DS) in 2017 relative to control wines. Gas chromatography-mass spectrometry (GC-MS) data showed higher relative levels of ethyl cinnamate and benzaldehyde in 100% WC wines and relative abundance of esters in DS wines. Descriptive sensory analysis showed that 100% WC additions led to vegetal, cooked fruit flavors, and spicy (clove) notes, whereas DS additions produced wines that were more herbal, fruity, and astringent, with lifted fruity notes related to esters, and astringency sensations related to enhanced tannin extraction from the stems. Both WC and DS additions led to variable increases in perceived astringency, suggesting that they can be used to add mouthfeel and improve texture in Pinot noir wines.
在美国加利福尼亚州埃德纳谷AVA连续两个年份的黑皮诺葡萄酒中,以商业规模研究了在50% (50% WC)和100% (100% WC)条件下添加干茎(DS)和整串葡萄酒的效果。添加100% WC可显著提高pH值和挥发性酸度。与酿酒处理相比,年份对花青素、聚合色素和颜色的影响更大,但对100% WC葡萄酒的花青素、聚合色素和颜色没有影响或有负面影响。相反,葡萄酒的单宁含量随着WC和茎干的添加比例而增加,2016年增加了68% (50% WC)、100% (100% WC)和90% (DS), 2017年增加了61% (50% WC)、123% (100% WC)和137% (DS)。气相色谱-质谱(GC-MS)分析表明,100% WC葡萄酒中肉桂酸乙酯和苯甲醛含量较高,DS葡萄酒中酯类含量相对较高。描述性感官分析表明,100% WC的添加会产生植物味、熟果味和辛辣(丁香)的味道,而DS的添加会产生更多的草药味、水果味和涩味,带有与酯类有关的水果味,以及与从茎中提取的单宁增强有关的涩味。WC和DS的添加都会导致感知到的涩味的不同程度的增加,这表明它们可以用来增加口感和改善黑皮诺葡萄酒的质地。
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引用次数: 11
A Novel Grape Downy Mildew Resistance Locus from Vitis rupestris 葡萄抗霜霉病新位点的研究
IF 1.9 3区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2020-08-20 DOI: 10.5344/ajev.2020.20030
Gaurab Bhattarai, A. Fennell, J. Londo, Courtney Coleman, L. Kovács
The viticulture industry needs advanced grape cultivars with genes that enhance disease resistance and environmental stress tolerance to meet the challenges of a changing climate. To discover beneficial allelic variants of grape genes, we established an F1 mapping population from a cross between two North American grapevines, Vitis rupestris Scheele and Vitis riparia Michx. We generated genotyping-by-sequencing (GBS) markers and constructed parental linkage maps consisting of 1177 and 1115 GBS markers, respectively (LOD threshold ≥ 14), which were validated by mapping the sex-determining locus to chromosome 2. Taking advantage of loci heterozygous in both parents, we also constructed an integrated map containing 2583 markers. We mapped a major quantitative trait locus (QTL) for downy mildew (Plasmopara viticola) resistance to chromosome 10 of V. rupestris using both greenhouse- and in vitro-generated leaf resistance data. This QTL explains 66.5% of the phenotypic variance under greenhouse conditions, and its 2-LOD confidence interval corresponds to region 2,470,297 to 3,024,940 bp on chromosome 10 in the Vitis vinifera L. PN40024 reference genome sequence (assembly 12X.v2). We provide PN40024-projected positions of the GBS markers, which can be used as anchors to develop additional markers for the introgression of this V. rupestris haplotype into cultivated grape varieties.
葡萄种植业需要先进的葡萄品种,这些品种具有增强抗病性和环境耐受性的基因,以应对气候变化的挑战。为了发现葡萄基因中有益的等位变异,我们从两种北美葡萄品种——葡萄(Vitis rupestris Scheele)和葡萄(Vitis riparia Michx)的杂交中建立了一个F1定位群体。我们生成了基因分型测序(GBS)标记,并构建了亲本连锁图谱,分别包含1177个和1115个GBS标记(LOD阈值≥14),通过将性别决定位点定位到2号染色体进行验证。利用双亲的位点杂合性,构建了包含2583个标记的综合图谱。利用温室和体外叶片抗霜霉病抗性数据,绘制了葡萄浆霉病对10号染色体抗性的主要数量性状位点(QTL)。该QTL解释了温室条件下66.5%的表型变异,其2-LOD置信区间对应于Vitis vinifera L. PN40024参考基因组序列(assembly 12X.v2) 10号染色体上2,470,297 ~ 3,024,940 bp区域。我们提供了GBS标记的pn40024投影位置,这些标记可以作为锚点,用于开发其他标记,以便将该葡萄葡萄单倍型导入栽培葡萄品种。
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引用次数: 10
期刊
American Journal of Enology and Viticulture
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