Veterinary medicine (Auckland, N.Z.)最新文献

Background: In Veterinary Medicine biochemical investigation of serum is widely used to aid diagnosis and treatment. However, delays usually happen between sampling and analysis. As a result, the serum is stored in refrigerators. In this regard, information on the effects of temperature and storage duration on the stability of the analyte is incomplete in general and its effect in sheep and goat serum is not described. Therefore, the objective of this study was to examine the stability of selected biochemical analytes from sheep and goat serum following storage at -20℃ for 2 months.

Methods: Serum from 20 apparently healthy male 2-2.5 year-old sheep and goats was obtained and aliquots of serum from each sample were kept in three tubes. The first tube is for baseline (T0), which is done within an hour, while the other two (T1 and T2) are stored at -20℃ for 1 and 2 months, respectively. Total protein, albumin, urea, total cholesterol, and triglycerides were assayed.

Results: The results revealed that storage temperature and duration for up to 2 months had no significant effect on any analytes except for urea in goats. The changes in terms of total observed error (TE_o) for total protein; albumin and urea were greater than the acceptable values in both animals.

Conclusion: Thus, further studies are required to assure alteration of analyte at various storage temperatures and duration. In addition, implementation of quality systems to achieve quality targets for analytes with greater TE_o as compared to the established TE_a is needed.

Background: In Ethiopia, anthrax is the second most important zoonotic disease, next to rabies. Data quantifying occurrence and distribution of animal anthrax in Awi administrative zone of Amhara region, Ethiopia, are limited. Thus, this study was conducted to describe the distribution of animal anthrax between 2011 and 2020 in Awi zone.

Methods: This study used secondary data of animal anthrax that occurred in the Awi zone and reported to the Regional and National Veterinary Authority between 2011 and 2020.

Results: A total of 1262 cases of anthrax in animals and 324 animals that died due to anthrax were reported. The highest number of anthrax cases were reported in 2012 (n = 671), sharing 48.9% of the 10-year animal anthrax reported. However, the highest number of animal death due to anthrax (n = 104) was reported in 2014. The overall case fatality rate of anthrax was 25.67% (n = 324). The highest animal anthrax cases (n = 984; 77.97%) and deaths (n = 259; 79.94%) were recorded in Bovine. The highest cases of anthrax were registered in May (n = 313), while no anthrax case was reported during December. The highest and lowest number of animal death due to anthrax were reported during July (n = 64) and January (n = 6), respectively. The highest number of anthrax cases was reported in the hot-dry season (n = 479; 37.96%) whereas the lowest was reported during the cold-dry season (n = 30; 2.38%).

Conclusion: The current study revealed a considerable number of animal anthrax cases and deaths in Awi zone every year. Hence, it is necessary for practicing prevention strategies including immunization programs before the peak season of anthrax outbreaks.

Introduction: Microbiological contamination in fish origin foods is the leading risk for public health. Among the range of pathogenic bacterial species that cause fish food borne diseases is Escherichia coli. The pathogenic strains of Escherichia coli cause diarrhea by producing and releasing toxins and can also be the cause of food spoilage in fish.

Methods: A cross-sectional study was conducted to assess hygienic practices of fish handlers, to evaluate bacterial load and antimicrobial resistance patterns of Escherichia coli along the fish value chain in Northwest Ethiopia. Systematic and purposive sampling techniques were used for uncooked and cooked fish samples respectively.

Results: From a total of 180 fish samples, 36 (20%) were positive for Escherichia coli. From 115 uncooked and 65 cooked fish samples examined, 27 (23.5%) and 9 (13.8%) had E. coli respectively. The highest mean bacterial count was observed in raw fish samples (6.13 × 10⁵ cfu/g), followed by cooked fish samples (2.81 × 10⁴ cfu/g). Among the interviewed fish handlers, 83.3%, 76.7% and 80% of respondents had good knowledge and attitude towards using a clean cutting-and-filleting board, storing raw and cooked foods separately and using an apron for reducing the risk of fish contamination, respectively. All 36 isolates were 100% sensitive to ciprofloxacin and gentamycin. Of the Escherichia coli isolates subjected to tetracycline, 55.6% were resistant, 8.3% were intermediate and 36.1% were susceptible.

Conclusion and recommendation: This study revealed that there was a lack hygienic practice and high Escherichia coli profiles were observed. Hence, it could be wise to advise the fish harvesters, fish traders, hotels and restaurants about fish food safety practices from harvesting to consumption to improve fish food safety practices and quality standards of fish harvested and sold in northwest Ethiopia.

Background: Aflatoxins (AFs) are major contaminants of feed used in the poultry industry that negatively affect animal and human health. In Ethiopia, previous studies on AFs mainly considered cattle feed and milk but scarce information exists for poultry feeds.

Methods: The aim of this study was to determine the occurrence of AFs in poultry feed in selected chicken rearing villages of Bishoftu. The study was conducted from December 2018 to May 2019. Thirty-three compound poultry feed samples were collected and analyzed for aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2) and total AFs (AFT) using high performance liquid chromatography (HPLC). The moisture content of the samples was also determined.

Results: The result indicated that 31 (94%) from a total of 33 samples were contaminated with AFs. The mean levels of AFB1, AFB2, AFG1, AFG2 and AFT were 70.11 µg/kg, 13.50 µg/kg, 88.55 µg/kg, 18.00 µg/kg and 190.18 µg/kg, respectively. This study found AFs at a level above the limit of FDA regulatory levels of 20 µg/kg in 25 (72.75%) samples for AFT and 22 (66.67%) samples for AFB1. The analysis of moisture content of the samples, ranges from 7.33% to 11.17%, indicating all were at optimal value (<12%).

Conclusion: The study showed the high contamination of AFs in poultry feeds with optimal moisture content and hence further investigations are needed to address the cause. The study also supports the need for preventive strategies of AFs contamination in poultry feeds in Bishoftu.

Background: Marek's disease virus is a devastating infection, causing high morbidity and mortality in chickens in Ethiopia.

Methods: The current study was conducted from March to November, 2021 with the general objective of performing antemortem and postmortem, isolation, and molecular detection of Marek's disease virus from outbreak cases in southwestern Ethiopia. Accordingly, based on outbreak information reported from the study sites namely, Bedelle, Yayo, and Bonga towns in southwestern Ethiopia, 50 sick chickens were sampled. The backyard and intensive farming systems of chickens were included in the sampling and priorities were given for chickens that showed clinical signs that are characteristics of Marek's disease.

Results: By clinical examinations, paralysis of legs and wings, gray eye, loss of weight, difficulty in breathing, and depression were recorded on all chickens sampled for this study and death of diseased chickens was observed. In addition, enlargement of the spleen and gross lesions of the liver and heart were recorded during postmortem examination. The death of infected chickens was observed in both vaccinated and non-vaccinated flocks. Out of 50 pooled feather follicle samples, Marek's disease virus was isolated from 14/50 (28%) by cell culture method and out of six tissue samples, the virus was isolated from 5/6(83.30%). By Real time polymerization chain reaction technique, which was targeted to detect the Meq gene, Marek's disease virus was detected from 18/50 feather follicles which accounts for 36% of sampled chickens.

Conclusion: In general, current study showed that the circulating Marek's disease virus in southwestern Ethiopia was caused by the oncogenic Gallid herpesvirus-2 (Serotype-1). Further research on molecular characterization of revolving virus in current and other regions is recommended for effective control of the disease through vaccination.

Background: Bluetongue (BT) disease is an arthropod-transmitted viral disease of domestic and wild ruminant species caused by Bluetongue virus (BTV). It is of most importance in sheep and endemic primarily in the tropical and subtropical regions where vectors (Culicoides species) are present.

Materials and methods: A cross-sectional study was conducted in July-November 2019 to examine the seroprevalence of BTV infection in ovine in Maji district of West Omo zone. Serum samples were examined for the presence of specific antibodies of BTV using competitive enzyme-linked immunosorbent assay (c-ELISA) test. The collected data was coded and analyzed using STATA version 13 software. Associations between sero-prevalence and its risk factors were tested in a Chi-square analysis and with a P<0.05 were considered as statistically significant.

Results: The individual animal prevalence was revealed as 39.23% (153/390). Herd size prevalence was: small size herd (37.42%; 61/163), medium size herd (32.35%; 55/170), and large size herd (64.91%; 37/57). Species-based prevalence showed ovine (38.00%; 141/371) and caprine (63.15%; 12/19). Age-based prevalence revealed adult (39.26%; 150/382) and young (37.5%; 3/8). The cumulative sex prevalence for both ovine and caprine was male (37.95%; 52/137) and female (39.92%; 101/253).

Conclusion: The current prevalence of BTV antibodies in the area was found to be high. Lack of application of bluetongue disease control mechanisms like vaccination for the animals is a key factors for the high prevalence of the disease in the areas besides the existence of the vectors.

Background: Neosporosis is a major cause of abortion in smallholder dairy farms in Ethiopia. However, its status and impact in pastoral cattle production settings were uncovered. This study was performed with the aims of estimating the seroprevalence and associated potential risk factors for Neospora caninum in Boran cattle in Teltelle district of Borana zone, Ethiopia.

Methods: 180 blood samples were collected from 48 randomly selected pastoral herds using a multistage sampling technique and subjected to an indirect enzyme-linked immunosorbent assay (ELISA) test to detect antibodies specific to N. caninum. A questionnaire survey was also used to identify the potential risk factors of N. caninum in the study area. Evaluation of the associated risk factors was conducted using a multivariable logistic regression model.

Results: Antibodies against N. caninum exposure were detected in 5% of cattle (95% CI: 1.816-8.184) from 180 animals tested. Similarly, the seroprevalence of N. caninum in herds with at least one positive animal was 14.6% (95% CI: 4.598-24.567) from 48 herds examined. A multivariable logistic regression model identified the following as significant risk factors: a history of abortion (AOR = 23; 95% CI: 2.354-188.702; P = 0.006), dystocia (AOR = 11; 95% CI = 22.275-55.860; P = 0.003), wells water sources (AOR = 9; 95% CI: 1.599-47.568; P = 0.012), and dogs fed with raw animal products (AOR = 6; 95% CI: 11.213-27.222; P = 0.028).

Conclusion: This study revealed the first serological evidence of N. caninum exposure in cattle reared under pastoral production system. Our findings suggest N. caninum is likely to be an important cause of abortion and dystocia in cattle in Ethiopia. Management practices, such as provision of hygienic water and restriction of dogs fed with raw animal products, are likely to reduce the risk of infection. Thus, maximizing community awareness about these disease management practices is suggested.

Introduction: Eimeria infection is one of the protozoal diseases of animals caused by various species of Eimeria (intracellular parasite) and causes reduced productivity and mortality in ruminants, especially in young ones. Despite the fact that the disease is one of the leading causes of economic losses, there is little information in Ethiopia on the occurrence of the infection in cattle and sheep.

Methods: A cross-sectional study was conducted from December 2021 to April 2022 in and around Adama and Bishoftu towns with the objectives to estimate the prevalence of Eimeria infection; identify circulating Eimeria oocysts, the intensity/burden of infection and associated risk factors of Eimeria infection in cattle and sheep. A total of 384 randomly selected (265 cattle and 119 sheep) fecal samples were collected from the rectum and examined by flotation technique using sheather's sugar solution to detect the oocysts of Eimeria. A 2.5% potassium dichromate solution was added to the positive fecal samples for sporulation of the oocysts.

Results and discussion: The overall prevalence of 48.95% Eimeria infection was recorded during the study. 45.0% and 58% prevalence of the infection was registered in cattle and sheep, respectively. There was a statistically significant difference (P ˂ 0.05) in Eimeria infection between the study animal species, age of the animals, breed, farm hygiene and management system. However, there was no significant difference in Eimeria infection (P > 0.05) in sex, body condition of the animals and fecal consistency. The maximum oocysts per gram of feces was found to be 10,000. Eimeria infection is of great importance to livestock producers and requires serious control and prevention initiatives.

Background: Trichostrongylus colubriformis, also called hairworm, is a genus of parasitic roundworm affecting gastro-intestinal tracts of a ruminant. Gross and microscopic lesion characterizations and comparing its effect in the small intestine of sheep and goats experimentally infected with T. colubriformis were undertaken in the study.

Methods: During the study period, 13 sheep and 14 goats were included in the experiment. The larvae of T. culibriformis were obtained from abattoirs and larvae were recovered by Bearmann techniques. The infective larvae of T. culibriformis (L3) as a single dose of 10,000 per-animal was administered orally to infected groups of sheep and goats. Blood was collected for hematological and serum biochemical analysis. Tissues for gross and histopathologic lesions characterization were collected from killed infected animals at 56 days.

Results: From the infected group, the total recovered mean worm burden was recorded as higher in goats (P<0.05) than sheep, with an establishment rate of 50.16% and 34.46%, respectively. The total mean PCV, Hb, and albumin values recorded in the infected groups of sheep and goats were significantly (P<0.05) lower than non-infected control of both animal groups. In goats, the total serum protein was significantly (P<0.05) lower in the infected group than the non-infected control group. Gross lesions found were enteritis with petechial hemorrhages, edema, hyperemia, and mucosal slough, which were marked in the duodenum (62.69%) and jejunum (33.33%) in sheep and 47.05% duodenum and 45.09% jejunum in goats. The microscopic lesions developed by T. colubriformis were subtotal villus atrophy, hemorrhage, straightened and elongated dilated crypts, loss of epithelium, mucosal erosion, and infiltration of inflammatory cells.

Conclusion: The present study showed that T. colubriformis infection caused physiological and pathological changes of the small-intestine in sheep and goats, with more severe infection in goats than sheep, although they were under the same management condition.

Introduction: Brucellosis is a neglected bacterial zoonosis with serious veterinary and public health importance throughout the world. A cross-sectional study on animal brucellosis was conducted aiming to estimate seroprevalence and molecular detection.

Methods: Blood samples were collected from a total of 4274 individual animals (cattle, small ruminants and camel) from 241 herds/flocks for serology and PCR. Serum samples were tested using multispecies I-ELISA. Blood clots from seropositive animals were also tested for brucellosis via PCR. Additionally, 13 vaginal swab samples were collected from animals (2 from bovine and 11 from small ruminants) with recent abortion history for bacterial isolation and molecular detection.

Results: The overall individual animal and herd level seroprevalence was 3.95% (169/4274) and 18.26% (44/241) respectively. The animal level seroprevalence at species level was 1.58% (47/2982), 8.89% (97/1091) and 12.44% (25/201) in bovine, small ruminants (sheep and goat) and camel, respectively. Herd level seroprevalence were 5.43% (10/184), 52.08% (25/48) and 100% (9/9) in bovine, small ruminant and camel, respectively. The animal level seroprevalence of bovine from intensive and extensive systems was 1.10% (31/2808) and 2.87% (5/174) respectively. Blood clots tested for brucellosis via PCR were negative by RT-PCR. Brucella species was isolated from 6/13 (46.15%) vaginal swab samples cultured on Brucella selective agar, and shown to be B. melitensis using Real-Time PCR.

Conclusion: Overall, seropositivity for camels was higher than what has been reported previously. Also, there was a notable difference in this study in cattle seroprevalence when comparing extensive with intensive systems, with the extensive system having much greater seropositivity.