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Multilayer monochromators for neutron scattering. 用于中子散射的多层单色仪。
Pub Date : 1976-05-01
A M Saxena, B P Schoenborn
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引用次数: 0
Neutron diffraction analysis of the structure of retinal photoreceptor membranes and rhodopsin. 视网膜感光膜和视紫红质结构的中子衍射分析。
Pub Date : 1976-05-01
M J Yeager
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引用次数: 0
Dynamical effects in small-angle neutron diffraction from membranes. 膜小角中子衍射的动力学效应。
Pub Date : 1976-05-01
D L Caspar, W C Phillips
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引用次数: 0
Investigation of biological macromolecular systems with a pulsed neutron source--a review. 脉冲中子源生物大分子体系的研究进展。
Pub Date : 1976-05-01
L Cser

The conclusion that can be drawn on the basis of the above considerations is that investigation of biological macromolecules and crystalline structures by SAS and diffraction of neutrons with the TOF method is feasible. The main difficulties of the TOF method (the wavelength dependence of the incident beam, resolution power, and detector efficiency; the need for their determination and up-to-date values) are compensated for by its advantages. Both methods allow a high data accumulation rate and optimal employment of the incident neutron spectrum. The latter has been achieved by utilizing a dominant part of the Maxwellian spectrum and by a more uniform distribution of statistical accuracy over the most informative measuring range. Another advantage is the high degree of monochronatization of the incident neutron beam by the TOF method. The rigid requirements concerning the data accumulation rate and the capacity of the on-line system computer memory are technical problems but not basic ones.

综上所述,利用SAS和中子衍射技术研究生物大分子和晶体结构是可行的。TOF方法的主要难点是入射光束的波长依赖性、分辨率和探测器效率;它的优点弥补了对它们的测定和最新值的需要。这两种方法都具有较高的数据积累率和入射中子谱的最佳利用。后者是通过利用麦克斯韦光谱的主要部分和在最具信息量的测量范围内统计精度的更均匀分布来实现的。TOF法的另一个优点是入射中子束的单时化程度高。在线系统对数据积累速率和计算机存储容量的严格要求是技术问题,而不是基本问题。
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引用次数: 0
Introduction to the theory of elastic neutron scattering. 弹性中子散射理论简介。
Pub Date : 1976-05-01
M Blume
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引用次数: 0
The production of deuterated E. coli. 氘化大肠杆菌的生产。
Pub Date : 1976-05-01
P B Moore, D M Engelman

Our results indicate that the preparation of deuterated macromolecules for neutron scattering from E. coli can be done easily and relatively cheaply. The entry of deuterium into macromolecules of differing species during cell growth can be controlled by varying the D2O content of the medium and the choice of carbon sources, all in minimal medium. Considering the range of deuterations obtainable with simple protonated carbon sources, there seems to be no reason to use deuterated carbon sources except for the rare occasions when a fully deuterated specimen is required.

我们的结果表明,从大肠杆菌中制备用于中子散射的氘化大分子是容易且相对便宜的。在细胞生长过程中,氘进入不同物种的大分子可以通过改变培养基的D2O含量和碳源的选择来控制,所有这些都是在最小的培养基中进行的。考虑到简单质子化碳源可获得的氘度范围,除了需要完全氘化样品的极少数情况外,似乎没有理由使用氘化碳源。
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引用次数: 0
Neutron scattering measurements with the label triangulation method on the 50 S subunit of E. coli ribosomes. 标签三角测量法对大肠杆菌核糖体50s亚基的中子散射测量。
Pub Date : 1976-05-01
W Hoppe, R May, P Stöckel, S Lorenz, V A Erdmann, H G Wittmann, H L Crespi, J J Katz, K Ibel

In the E. coli 50 S ribosomal subunit, proteins L7/L12 and L10 were deuterated by partial reconstitution. The distance between L7/L12 and L10 was measured by the label triangulation method and was found to be approximately 100 A or, with low probability, 60 to 70 A, depending on the concentration.

在大肠杆菌50s核糖体亚基中,L7/L12和L10蛋白通过部分重构被氘化。L7/L12和L10之间的距离通过标记三角测量法测量,发现根据浓度的不同,L7/L12和L10之间的距离约为100 A,或者有低概率为60至70 A。
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引用次数: 0
Structure and function in biology. 生物学中的结构和功能。
Pub Date : 1976-05-01
C H Hirs
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引用次数: 0
Linear position-sensitive counter system for protein crystallography. 用于蛋白质晶体学的线性位置敏感计数器系统。
Pub Date : 1976-05-01
J E Cain, J C Norvell, B P Schoenborn
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引用次数: 0
Data analysis of low-angle diffraction by.one-dimensional crystals. 低角衍射的数据分析。一维的晶体。
Pub Date : 1976-05-01
M J Yeager
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引用次数: 0
期刊
Brookhaven symposia in biology
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