Pub Date : 2005-12-01DOI: 10.11263/JSOTP1982.24.81
T. Matsuno, Takahiro Miyai, Gaku Tamazawa, Kazuki Kitahara, T. Miyasaka, Kazuhiko Omata, Chiaki Arai, T. Satoh
Platelet-rich plasma (PRP), which is obtained by centrifuging autologous blood, contains a large number of platelets. When platelets are activated using substances such as thrombin, ƒ¿ granules release platelet released growth factor (PRGF) such as platelet-derived growth factor (PDGF) and transforming growth factor-ƒÀ (TGF-ƒÀ). Since these growth factors promote proliferation and differentiation of cells that are required for tissue regeneration, PRP has been used for bone and periodontal tissue regeneration in clinical dentistry. In the present study, we investigated the changes in PDGF-AB levels before and after platelet activation using ELISA. In addition, cellular proliferation was investigated by adding platelet released supernatant (PRS) produced by activating platelets of PRP to mesenchymal stem cell (MSC) culture media using MTS assay. The results showed that the level of PDGF-AB in PRS was 5.31 times higher than that before platelet activation. Furthermore, adding PRP or PRS significantly promoted MSC proliferation compared to the control, and adding PRS enhanced cellular proliferation in a dose-dependent manner from days 1 to 4 of culture. This study suggested that activation of platelets in PRP releases and concentrates PRGF, and that adding PRS to MSC promotes early cellular proliferation of MSC.
{"title":"Effects of Platelet-released Supernatant on Mesenchymal Stem Cell Proliferation","authors":"T. Matsuno, Takahiro Miyai, Gaku Tamazawa, Kazuki Kitahara, T. Miyasaka, Kazuhiko Omata, Chiaki Arai, T. Satoh","doi":"10.11263/JSOTP1982.24.81","DOIUrl":"https://doi.org/10.11263/JSOTP1982.24.81","url":null,"abstract":"Platelet-rich plasma (PRP), which is obtained by centrifuging autologous blood, contains a large number of platelets. When platelets are activated using substances such as thrombin, ƒ¿ granules release platelet released growth factor (PRGF) such as platelet-derived growth factor (PDGF) and transforming growth factor-ƒÀ (TGF-ƒÀ). Since these growth factors promote proliferation and differentiation of cells that are required for tissue regeneration, PRP has been used for bone and periodontal tissue regeneration in clinical dentistry. In the present study, we investigated the changes in PDGF-AB levels before and after platelet activation using ELISA. In addition, cellular proliferation was investigated by adding platelet released supernatant (PRS) produced by activating platelets of PRP to mesenchymal stem cell (MSC) culture media using MTS assay. The results showed that the level of PDGF-AB in PRS was 5.31 times higher than that before platelet activation. Furthermore, adding PRP or PRS significantly promoted MSC proliferation compared to the control, and adding PRS enhanced cellular proliferation in a dose-dependent manner from days 1 to 4 of culture. This study suggested that activation of platelets in PRP releases and concentrates PRGF, and that adding PRS to MSC promotes early cellular proliferation of MSC.","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"43 1","pages":"154-157"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63819284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Kakimoto, H. Numasawa, N. Yamamoto, E. Takeda, T. Yamauchi, T. Shibahara
Frequent allelic imbalances, including loss of heterozygosity (LOH) and microsatellite instability (MSI), have been found on the long arm of chromosome 2 (2q) in several types of human cancer. This study was designed to identify the tumor suppressor locus (or loci) associated with oral squamous cell carcinoma (SCC) on 2q. To better understand the details of genetic alterations on 2q, we performed polymerase chainreaction analysis of microsatellite polymorphisms corresponding to 10 loci on this chromosome. We identified a novel tumor suppressor locus in this region in primary oral SCCs. To further determine the role of 2q deletions in oral carcinogenesis, 19 oral SCCs (19 sets of primary and corresponding normal tissues) were examined for allelic imbalances (LOH or MSI) on 2q, using ten microsatellite markers. Among these 19 patients, 11 (57.9%) showed LOH at one or more loci. Deletion mapping of these tumors revealed four discrete, commonly deleted regions on the chromosome arm. Furthermore, we detected MSI in 4 of the patients (21.1 %).We compared our results with clinicopathologic features. A number of sites with LOH on 2q were detected in early stage lesions, and the frequency of LOH was slightly but not significantly higher in later clinical stages. Our results suggest that allelic imbalances on 2q are involved in the development of oral SCC and that one or more putative tumor suppressor genes contributing to the pathogenesis of this disease are present on 2q.
{"title":"Loss of Heterozygosity and Microsatellite Instability on the Long Arm of Chromosome 2 in Human Oral Squamous Cell Carcinoma","authors":"Y. Kakimoto, H. Numasawa, N. Yamamoto, E. Takeda, T. Yamauchi, T. Shibahara","doi":"10.5794/JJOMS.51.374","DOIUrl":"https://doi.org/10.5794/JJOMS.51.374","url":null,"abstract":"Frequent allelic imbalances, including loss of heterozygosity (LOH) and microsatellite instability (MSI), have been found on the long arm of chromosome 2 (2q) in several types of human cancer. This study was designed to identify the tumor suppressor locus (or loci) associated with oral squamous cell carcinoma (SCC) on 2q. To better understand the details of genetic alterations on 2q, we performed polymerase chainreaction analysis of microsatellite polymorphisms corresponding to 10 loci on this chromosome. We identified a novel tumor suppressor locus in this region in primary oral SCCs. To further determine the role of 2q deletions in oral carcinogenesis, 19 oral SCCs (19 sets of primary and corresponding normal tissues) were examined for allelic imbalances (LOH or MSI) on 2q, using ten microsatellite markers. Among these 19 patients, 11 (57.9%) showed LOH at one or more loci. Deletion mapping of these tumors revealed four discrete, commonly deleted regions on the chromosome arm. Furthermore, we detected MSI in 4 of the patients (21.1 %).We compared our results with clinicopathologic features. A number of sites with LOH on 2q were detected in early stage lesions, and the frequency of LOH was slightly but not significantly higher in later clinical stages. Our results suggest that allelic imbalances on 2q are involved in the development of oral SCC and that one or more putative tumor suppressor genes contributing to the pathogenesis of this disease are present on 2q.","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"43 1","pages":"70-73"},"PeriodicalIF":0.0,"publicationDate":"2005-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71043579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fas is widely expressed on the cell surface of many normal and neoplastic cells and induces apoptotic cell death in the presence of Fas ligand (FasL) or Fas-agonistic antibody CH11 (Fas-mediated apoptosis) Cancer cells constitutively expressing Fas are generally resistant to Fas-mediatedapoptosis. Recently, it has been reported that interferon-ƒÁ (IFN-ƒÁ) enhances Fas-mediated apoptosis. An oral squamous cell carcinoma cell line, NA, also constitutively expresses Fas on the plasma membrane and shows low susceptibility to Fas-agonistic antibody-induced apoptosis. This study was conducted to examine the effects of IFN-ƒÁy on Fas-mediated apoptosis, the expression of Fas and FasL, and the production of soluble Fas (sFas) in NA cells. The results revealed that a Fas-agonistic antibody, CH11, induced apoptosis of NA cells and IFN-ƒÁ enhanced susceptibility of NA cells to CH11-induced apoptosis. Further more, IFN-ƒÁ up-regulated Fas expression on NA cells without affecting the expression of FasL. A slight decrease in sFas expression was induced by treatment with IFN-ƒÁ. These results suggest that IFN-ƒÁ may enhance the susceptibility of NA cells to Fas-mediated apoptosis through the up-regulation of Fas.
{"title":"Enhanced Susceptibility to Fas-mediated Apoptosis by Interferon-γ in an Oral Squamous Cell Carcinoma Cell Line","authors":"T. Takemi, K. Takizawa, M. Iwase, M. Nagumo","doi":"10.5794/JJOMS.50.629","DOIUrl":"https://doi.org/10.5794/JJOMS.50.629","url":null,"abstract":"Fas is widely expressed on the cell surface of many normal and neoplastic cells and induces apoptotic cell death in the presence of Fas ligand (FasL) or Fas-agonistic antibody CH11 (Fas-mediated apoptosis) Cancer cells constitutively expressing Fas are generally resistant to Fas-mediatedapoptosis. Recently, it has been reported that interferon-ƒÁ (IFN-ƒÁ) enhances Fas-mediated apoptosis. An oral squamous cell carcinoma cell line, NA, also constitutively expresses Fas on the plasma membrane and shows low susceptibility to Fas-agonistic antibody-induced apoptosis. This study was conducted to examine the effects of IFN-ƒÁy on Fas-mediated apoptosis, the expression of Fas and FasL, and the production of soluble Fas (sFas) in NA cells. The results revealed that a Fas-agonistic antibody, CH11, induced apoptosis of NA cells and IFN-ƒÁ enhanced susceptibility of NA cells to CH11-induced apoptosis. Further more, IFN-ƒÁ up-regulated Fas expression on NA cells without affecting the expression of FasL. A slight decrease in sFas expression was induced by treatment with IFN-ƒÁ. These results suggest that IFN-ƒÁ may enhance the susceptibility of NA cells to Fas-mediated apoptosis through the up-regulation of Fas.","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"42 1","pages":"69-72"},"PeriodicalIF":0.0,"publicationDate":"2004-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71043556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-08-01DOI: 10.11263/JSOTP1982.23.54
Isao Hasegawa, T. Satoh, I. Kobayashi
{"title":"Molecular Biological Investigation in Candida Albicans Isolated from Recurrent Candidiasis of the Tongue","authors":"Isao Hasegawa, T. Satoh, I. Kobayashi","doi":"10.11263/JSOTP1982.23.54","DOIUrl":"https://doi.org/10.11263/JSOTP1982.23.54","url":null,"abstract":"","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"42 1","pages":"156-159"},"PeriodicalIF":0.0,"publicationDate":"2004-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63818918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hidetoshi Ito, K. Tsuchikawa, H. Katsuragi, Kazuko Saito
{"title":"Effect of Calcium Channel Blockers on Proliferation of Human Gingival Fibroblast (Gin-1)","authors":"Hidetoshi Ito, K. Tsuchikawa, H. Katsuragi, Kazuko Saito","doi":"10.11263/JSOTP1982.23.1","DOIUrl":"https://doi.org/10.11263/JSOTP1982.23.1","url":null,"abstract":"","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"42 1","pages":"153-155"},"PeriodicalIF":0.0,"publicationDate":"2004-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63818231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-03-01DOI: 10.1201/9781498713535-22
M. Ueda
{"title":"Maxillofacial Bone Regeneration Using Tissue Engineering Concepts","authors":"M. Ueda","doi":"10.1201/9781498713535-22","DOIUrl":"https://doi.org/10.1201/9781498713535-22","url":null,"abstract":"","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"39 1","pages":"199-207"},"PeriodicalIF":0.0,"publicationDate":"2003-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65970617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The objective of this study was to investigate the characteristics of osteogenesis around dental implants in both vascularized and nonvascularized bone grafts. Vascularized bone grafts were nourished by inferior alveolar vessels providing medullary blood flow to the mandibles of adult dogs. Titanium implants were placed into the bone grafts and normal mandibles. The time course of osteogenesis around the implants was observed histologically from 2 to 24 weeks after operation. Specimers stained with Stevenel's blue and Van Gieson-picrofuchsin stains and fluorescently labeled specimens were examined with a confocal laser scanning microscope. Implant-bone contact rates were determined.In vascularized bone grafts, new bone formation was evident all around the implants at 2 weeks postoperatively. New bone became compact with the passage of time. These processes of new bone formation were similar to those of normal mandibles.In cortical bone of vascularized bone grafts, bone resorption was recognized. However, bone remodeling was found at an early stage, suggesting that implants could be placed into vascularized bone grafts immediately after the primary operation.In nonvascularized bone grafts, bone resorption and remodeling occurred from the outside of cortical bone. New bone formation around implants was found at 8 weeks postoperatively. The implant apex was encapsulated by fibrous tissue at 24 weeks postoperatively. The results suggest that simultaneous implantation with free bone grafting requires a long time for bony linkage and that encapsulation of implants by fibrous tissue may occur in nonvascularized bone grafts.
{"title":"Characteristics of Osteogenesis Around Dental Implants Inserted into Vascularized Bone Grafts and Free Bone Grafts in Mandible of Dogs","authors":"Yoshitaka Furuya, Y. Yajima, H. Noma","doi":"10.5794/JJOMS.48.557","DOIUrl":"https://doi.org/10.5794/JJOMS.48.557","url":null,"abstract":"The objective of this study was to investigate the characteristics of osteogenesis around dental implants in both vascularized and nonvascularized bone grafts. Vascularized bone grafts were nourished by inferior alveolar vessels providing medullary blood flow to the mandibles of adult dogs. Titanium implants were placed into the bone grafts and normal mandibles. The time course of osteogenesis around the implants was observed histologically from 2 to 24 weeks after operation. Specimers stained with Stevenel's blue and Van Gieson-picrofuchsin stains and fluorescently labeled specimens were examined with a confocal laser scanning microscope. Implant-bone contact rates were determined.In vascularized bone grafts, new bone formation was evident all around the implants at 2 weeks postoperatively. New bone became compact with the passage of time. These processes of new bone formation were similar to those of normal mandibles.In cortical bone of vascularized bone grafts, bone resorption was recognized. However, bone remodeling was found at an early stage, suggesting that implants could be placed into vascularized bone grafts immediately after the primary operation.In nonvascularized bone grafts, bone resorption and remodeling occurred from the outside of cortical bone. New bone formation around implants was found at 8 weeks postoperatively. The implant apex was encapsulated by fibrous tissue at 24 weeks postoperatively. The results suggest that simultaneous implantation with free bone grafting requires a long time for bony linkage and that encapsulation of implants by fibrous tissue may occur in nonvascularized bone grafts.","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"40 1","pages":"63-66"},"PeriodicalIF":0.0,"publicationDate":"2002-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71043164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Kido, M. Noguchi, H. Kinjyo, Tatsuru Suyama, H. Kubota, A. Miyazaki, G. Kohama
The purpose of this study was to assess changes in prognostic factors related to modification of surgical treatment on the basis of grade of tumor malignancy and to contribute to improved surgical treatment of patients with oral squamous cell carcinoma. Fourhundred three patients with oral squamous cell carcinoma treated by surgery were classified according to treatment period as follows: f irst period, 1976-1986; second period, 1987-1991; and third period, 1992-1997.Three and 5-year cumulative disease-specific survival rates according to treatment period were as follows: first period (n=187), 78.6, 76.3%; second period (n=113), 76.6, 73.7%; and third period (n=103), 88.9, 87.2%. On univariate analysis of survival according to T, N, stage, growth pattern, and mode of invasion, significant differences (P<0.05) were found for all characteristics in the first and second periods, while in the third period no significant difference was found for growth pattern or mode of invasion.Cox regression analysis of disease-specific survival identified the following as independent prognostic factors: mode of invasion (1+2, 3, 4C+4D), growth pattern (exophytic, endophytic), and N (0, 1, 2) in the first period, mode of invasion (1-3, 4C+4D) and N (0, 1, 2+3) in the second period, and only N (0, 1, 2) in the third period.Owing to the improved outcome for highly invasive carcinoma, the prognostic values during the third period differed from those during the first and second periods.
{"title":"Prognostic Factors for Survival According to Treatment Period in Oral Squamous Cell Carcinomas","authors":"Y. Kido, M. Noguchi, H. Kinjyo, Tatsuru Suyama, H. Kubota, A. Miyazaki, G. Kohama","doi":"10.5794/JJOMS.47.331","DOIUrl":"https://doi.org/10.5794/JJOMS.47.331","url":null,"abstract":"The purpose of this study was to assess changes in prognostic factors related to modification of surgical treatment on the basis of grade of tumor malignancy and to contribute to improved surgical treatment of patients with oral squamous cell carcinoma. Fourhundred three patients with oral squamous cell carcinoma treated by surgery were classified according to treatment period as follows: f irst period, 1976-1986; second period, 1987-1991; and third period, 1992-1997.Three and 5-year cumulative disease-specific survival rates according to treatment period were as follows: first period (n=187), 78.6, 76.3%; second period (n=113), 76.6, 73.7%; and third period (n=103), 88.9, 87.2%. On univariate analysis of survival according to T, N, stage, growth pattern, and mode of invasion, significant differences (P<0.05) were found for all characteristics in the first and second periods, while in the third period no significant difference was found for growth pattern or mode of invasion.Cox regression analysis of disease-specific survival identified the following as independent prognostic factors: mode of invasion (1+2, 3, 4C+4D), growth pattern (exophytic, endophytic), and N (0, 1, 2) in the first period, mode of invasion (1-3, 4C+4D) and N (0, 1, 2+3) in the second period, and only N (0, 1, 2) in the third period.Owing to the improved outcome for highly invasive carcinoma, the prognostic values during the third period differed from those during the first and second periods.","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"39 1","pages":"76-79"},"PeriodicalIF":0.0,"publicationDate":"2001-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71043098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Uzawa, D. Akanuma, A. Negishi, T. Amagasa, Mitsuaki Yoshida
Cytogenetic and molecular studies have indicated that a putative tumor suppresor gene (s), which may play an important role in oral squamous cell carcinogenesis, is located on the short arm of chromosome 3 (3 p). We analyzed loss of heterozygosity (LOH) on 3 p, especially the 3 p25-pter region, in 17 oral squamous cell carcinomas (OSCCs) with the use of 5 microsatellite markers and constructed a deletion map for this chromosome arm. LOH at one or more loci was detected in 9 of 17 (53%) tumors. LOH on these region was more common in advanced OSCCs and may be associated with tumor progression. Our data support the notion that one of tumor suppressor gene (s) contributing to the progression of oral squamous cell carcinoma resides on 3 p25-pter.
{"title":"Loss of Heterozygosity on the Short Arm of Chromosome 3 in Oral Squamous Cell Carcinomas : Relationship between loss of heterozygosity on 3p25-ter region and clinical and histological features","authors":"N. Uzawa, D. Akanuma, A. Negishi, T. Amagasa, Mitsuaki Yoshida","doi":"10.5794/JJOMS.46.455","DOIUrl":"https://doi.org/10.5794/JJOMS.46.455","url":null,"abstract":"Cytogenetic and molecular studies have indicated that a putative tumor suppresor gene (s), which may play an important role in oral squamous cell carcinogenesis, is located on the short arm of chromosome 3 (3 p). We analyzed loss of heterozygosity (LOH) on 3 p, especially the 3 p25-pter region, in 17 oral squamous cell carcinomas (OSCCs) with the use of 5 microsatellite markers and constructed a deletion map for this chromosome arm. LOH at one or more loci was detected in 9 of 17 (53%) tumors. LOH on these region was more common in advanced OSCCs and may be associated with tumor progression. Our data support the notion that one of tumor suppressor gene (s) contributing to the progression of oral squamous cell carcinoma resides on 3 p25-pter.","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"38 1","pages":"63-66"},"PeriodicalIF":0.0,"publicationDate":"2000-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71043036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1999-04-01DOI: 10.11263/JSOTP1982.19.28
Kazuki Kitahara, T. Satoh
{"title":"Effects of Antibiotics on Phagocytosis of Polymorphonuclear Leukocytes in Experimental Rabbit Infection Models","authors":"Kazuki Kitahara, T. Satoh","doi":"10.11263/JSOTP1982.19.28","DOIUrl":"https://doi.org/10.11263/JSOTP1982.19.28","url":null,"abstract":"","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"38 1","pages":"146-149"},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63815771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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