Pub Date : 2017-01-01DOI: 10.7551/mitpress/11438.003.0014
Paulo F. Almeida
E pur si muove (and yet it moves) is a comment attributed to Galileo after the Catholic Church forced him to recant his theory that the earth moved around the sun. In this issue of the Biophysical Journal, Fuselier and Wimley (1) report the discovery of an amino acid sequence motif that appears to impart to polypeptides the ability to translocate across lipid membranes silently—that is, without causing flux of aqueous solutes across the membrane. This work follows a series of articles from Wimley’s laboratory on methods to reliably measure translocation of membraneactive peptides across phospholipid membranes, and on the use of an orthogonal high-throughput assay to screen combinatorial libraries of membrane-active peptides to specifically select for those peptides that spontaneously translocate but do not permeabilize membranes to water-soluble molecules (2–4). The translocating peptide TP2 emerged from this selection procedure as one of the most effective (2). TP2 is a 13-residue cationic peptide with the sequence PLIYLRLLRGQWC-amide. It was shown to cross the membranes of lipid vesicles and cells, even with polar fluorescent dyes attached (2,5). Wimley and co-workers noticed that the central
E pur si muove(但它还在运动)是伽利略在天主教会迫使他放弃地球绕太阳转的理论后所说的话。在这一期的《生物物理杂志》上，Fuselier和Wimley(1)报告了一项氨基酸序列基序的发现，该基序似乎赋予了多肽在脂质膜上无声转移的能力——也就是说，不会引起溶质在膜上的通量。这项工作遵循了Wimley实验室关于可靠测量膜活性肽跨磷脂膜易位的方法的一系列文章，以及使用正交高通量试验筛选膜活性肽组合文库，以特别选择那些自发易位但不使膜渗透到水溶性分子的肽(2-4)。易位肽TP2从这个选择过程中脱颖而出，成为最有效的选择之一(2)。TP2是一个有13个残基的阳离子肽，序列为PLIYLRLLRGQWC-amide。它被证明可以穿过脂质囊泡和细胞的膜，即使有极性荧光染料附着(2,5)。温利和同事们注意到中央
{"title":"New and Notable And Yet It Moves","authors":"Paulo F. Almeida","doi":"10.7551/mitpress/11438.003.0014","DOIUrl":"https://doi.org/10.7551/mitpress/11438.003.0014","url":null,"abstract":"E pur si muove (and yet it moves) is a comment attributed to Galileo after the Catholic Church forced him to recant his theory that the earth moved around the sun. In this issue of the Biophysical Journal, Fuselier and Wimley (1) report the discovery of an amino acid sequence motif that appears to impart to polypeptides the ability to translocate across lipid membranes silently—that is, without causing flux of aqueous solutes across the membrane. This work follows a series of articles from Wimley’s laboratory on methods to reliably measure translocation of membraneactive peptides across phospholipid membranes, and on the use of an orthogonal high-throughput assay to screen combinatorial libraries of membrane-active peptides to specifically select for those peptides that spontaneously translocate but do not permeabilize membranes to water-soluble molecules (2–4). The translocating peptide TP2 emerged from this selection procedure as one of the most effective (2). TP2 is a 13-residue cationic peptide with the sequence PLIYLRLLRGQWC-amide. It was shown to cross the membranes of lipid vesicles and cells, even with polar fluorescent dyes attached (2,5). Wimley and co-workers noticed that the central","PeriodicalId":76292,"journal":{"name":"Osterreichische Krankenpflegezeitschrift","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71229881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Web-publishing on the Internet].","authors":"J Ehmsen","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76292,"journal":{"name":"Osterreichische Krankenpflegezeitschrift","volume":"53 6-7","pages":"28-31"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21805738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Respect for wonders and mysteries of life: Jean Watson's theory on patient care].","authors":"P Laudert","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76292,"journal":{"name":"Osterreichische Krankenpflegezeitschrift","volume":"53 6-7","pages":"32-4"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21805740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[On the topic of grand rounds--relaxing dialog or uneasy situation?].","authors":"M Gogg, A Vogel","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76292,"journal":{"name":"Osterreichische Krankenpflegezeitschrift","volume":"53 5","pages":"12"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21750611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Professional development of the \"nurse\" from 1850-1950. A historical paper on the development of nursing].","authors":"M Gattringer","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76292,"journal":{"name":"Osterreichische Krankenpflegezeitschrift","volume":"53 5","pages":"30-3, 39"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21750622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：