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Rivista di istochimica, normale e patologica最新文献

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Spontaneous release of DNA by living cells [proceedings]. 活细胞自发释放DNA[学报]。
Rivista di istochimica, normale e patologica
Pub Date : 1976-01-01
P Anker, M Stroum, P Maurice
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引用次数: 0
Cytochemical investigation on lipids in human major salivary glands. A light and electron microscope study. 人主要唾液腺脂质细胞化学研究。光学和电子显微镜研究
Rivista di istochimica, normale e patologica
Pub Date : 1976-01-01
P Sirigu, G Diaz, M S Lantini, M Del Fiacco

The localization of lipids in the secretion of human parotid and submandibular glands has been here investigated. Ultrastructural observations following lipid extraction and histochemical data showed that lipid components are not present in the secretory granules. In fact, while histochemical reactions are completely negative, secretory granules from lipid-depleted glands retain all their morphological characters. These results led us to emphasize that, at least in human seromucous cells, lipids are not directly involved in the salivary secretion. This conclusion, which is supported by the biochemical data concerning the lipid content in human saliva, disagrees with the findings obtained in rat and guinea pig salivary glands. However, this discrepancy may be ascribed to the specific biochemical and ultrastructural differences found in the salivary glands of the various mammalian species.

脂质在人腮腺和下颌骨腺分泌中的定位已被研究。脂质提取后的超微结构观察和组织化学数据显示，脂质成分不存在于分泌颗粒中。事实上，当组织化学反应完全阴性时，脂质耗尽腺体的分泌颗粒保留了其所有形态特征。这些结果使我们强调，至少在人类浆液细胞中，脂质并不直接参与唾液分泌。这一结论得到了有关人类唾液脂质含量的生化数据的支持，但与在大鼠和豚鼠唾液腺中获得的结果不一致。然而，这种差异可能归因于在不同哺乳动物物种的唾液腺中发现的特定生化和超微结构差异。
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引用次数: 0
Effect of glucagon on synthesis and turnover of DNA in foetal hepatic nuclei of rats (proceedings). 胰高血糖素对大鼠胎儿肝核DNA合成和转换的影响(学报)。
Rivista di istochimica, normale e patologica
Pub Date : 1976-01-01
R Rossi, M P Viola-Magni
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引用次数: 0
The mode of growth within the jejunal crypts of the rat [proceedings]. 大鼠空肠隐窝内的生长模式[学报]。
Rivista di istochimica, normale e patologica
Pub Date : 1976-01-01
W Maurer
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引用次数: 0
Do leukaemic lymphocytes differ from normal lymphocytes in their ability to repair DNA damage [proceedings]? 白血病淋巴细胞在修复DNA损伤的能力上与正常淋巴细胞不同吗?
Rivista di istochimica, normale e patologica
Pub Date : 1976-01-01
P A Maurice, C Lederrey
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引用次数: 0
[Genome dimensions of some species of reptiles [proceedings]. 一些爬行动物物种的基因组尺寸[学报]。
Rivista di istochimica, normale e patologica
Pub Date : 1976-01-01
E Olmo
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引用次数: 0
[Existence of 2 different morphofunctional aspects of the Purkinje cells in the cerebellum of adult rats [proceedings]. 成年大鼠小脑浦肯野细胞两种不同形态功能方面的存在[论文集]。
Rivista di istochimica, normale e patologica
Pub Date : 1976-01-01
G Bernocchi, M L De Vivo, G F De Stefano
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引用次数: 0
Development and metamorphosis of the larvae of Ascidia malaca in different experimental conditions. 不同实验条件下马来海鞘幼虫的发育和变态。
Rivista di istochimica, normale e patologica
Pub Date : 1976-01-01
G Materazzi, L Vitaioli, A M Bondi

In larvae of Ascidia malaca that have developed in sea-water without sulphate ions, the cells of the caphalo-ventral region are devoid of sulphated mucopolysaccharides, and the chordal and muscular cells of the tail turn out to be altered; after metamorphosis no adhesive ampullae appear. The addition of cysteine to water lacking sulphate ions leads to a normal synthesis of sulphated mucopolysaccharides in the cells of the cephalo-ventral region, and to a normal secretion of matrix by the chordal cells; but the larvae do not regain the ability to swim. The addition of N-dichloroacetyl-DL-phenylalanine to sea-water does not alter the synthesis of sulphated mucopolysaccharides in the cells of the cephalo-ventral region, but, probably by acting as a competitive inhibitor of phenylalanine, it causes alterations in the muscular cells of the tail.

在没有硫酸盐离子的海水中发育的海鞘幼虫，头腹区细胞缺乏硫酸盐粘多糖，尾索细胞和肌肉细胞发生改变;变态后无粘连壶腹。在缺乏硫酸盐离子的水中添加半胱氨酸可导致头腹区细胞中硫酸盐粘多糖的正常合成，并可导致脊索细胞正常分泌基质;但是幼虫不能重新获得游泳的能力。在海水中加入n -二氯乙酰- dl -苯丙氨酸不会改变头腹区细胞中硫酸粘多糖的合成，但可能由于它作为苯丙氨酸的竞争性抑制剂而引起尾部肌肉细胞的变化。
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引用次数: 0
[Quantitative limits of the Feulgen reaction: analysis of interference caused by dehistonization and denaturation and renaturation treatments]. [Feulgen反应的定量界限:脱组织和变性、复性处理的干扰分析]。
Rivista di istochimica, normale e patologica
Pub Date : 1976-01-01
C A Redi

The Feulgen reaction intensity (measured with a microdensitometer Vickers M86 on the nucleus of erytrocytes of Xenopus laevis Daud.) is increased after dehistonization according to Brody (1974) only if the dehistonization is made before the fixation in acetic acid. The denaturation and renaturation treatments which should act specifically on the screws of the DNA and therefore should not affect the Feulgen reaction, act in a specific manner, probably going away another histonic components. On the dehistonized material the action of the hydrolysis of the Feulgen reaction would add up to that implicit in the dehistonization treatment and would cause a rapid fall of the values for loss of material as consequence of depolymerization facts, according to Andersson and Kjellstrand (1975). The successive renaturation treatment both on dehistonized and on non dehistonized material does not change significantly the values precedently obtained and this confirms the idea that the rilevability of the Feulgen reaction is not influenced by the treatments "per se" but by the deep "touching" of the chromatin components.

根据Brody(1974)的研究，只有在醋酸固定之前进行去组织处理，去组织处理后Feulgen反应强度(用Vickers M86微密度计在非洲爪蟾的红细胞核上测量)才会增加。变性和再变性处理应该对DNA的螺丝钉起作用因此不应该影响Feulgen反应，以一种特定的方式起作用，可能会去除另一种组织成分。根据Andersson和Kjellstrand(1975)的说法，在去组化的材料上，Feulgen反应的水解作用加起来会导致去组化处理中隐含的结果，并且会导致解聚事实导致的材料损失值的迅速下降。对去组化和非去组化材料的连续复性处理不会显著改变先前获得的值，这证实了Feulgen反应的可还原性不受处理“本身”的影响，而是受染色质组分的深度“接触”的影响。
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引用次数: 0
The bursa of Fabricius in birds. Recent histochemical aspects of its differentiation towards immunological competence. 法氏囊鸟类的法氏囊其向免疫能力分化的组织化学方面的最新进展。
Rivista di istochimica, normale e patologica
Pub Date : 1976-01-01
F Rossi, F D'Anna
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引用次数: 0
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