Pub Date : 2007-01-01DOI: 10.1007/978-0-387-34504-8_1
Insoon Kim, Ken Kobayashi, Euna Cho, Patricia C Zambryski
{"title":"Regulation of plant intercellular communication via plasmodesmata.","authors":"Insoon Kim, Ken Kobayashi, Euna Cho, Patricia C Zambryski","doi":"10.1007/978-0-387-34504-8_1","DOIUrl":"https://doi.org/10.1007/978-0-387-34504-8_1","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"28 ","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26429928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-01-01DOI: 10.1007/978-0-387-34504-8_5
Ola Söderberg, Karl-Johan Leuchowius, Masood Kamali-Moghaddam, Malin Jarvius, Sigrun Gustafsdottir, Edith Schallmeiner, Mats Gullberg, Jonas Jarvius, Ulf Landegren
Knowledge about the total human genome sequence now provides opportunities to study its myriad gene products. However, the presence of alternative splicing, post-translational modifications, and innumerable protein-protein interactions among proteins occurring at widely different concentrations, all combine to place extreme demands on the specificity and sensitivity of assays. The choice of method also depends on matters such as whether proteins will be analyzed in body fluids and lysates, or localized inside single cells. In this review we discuss commonly used detection methods and compare these to the recently-developed proximity ligation technique.
{"title":"Proximity ligation: a specific and versatile tool for the proteomic era.","authors":"Ola Söderberg, Karl-Johan Leuchowius, Masood Kamali-Moghaddam, Malin Jarvius, Sigrun Gustafsdottir, Edith Schallmeiner, Mats Gullberg, Jonas Jarvius, Ulf Landegren","doi":"10.1007/978-0-387-34504-8_5","DOIUrl":"https://doi.org/10.1007/978-0-387-34504-8_5","url":null,"abstract":"<p><p>Knowledge about the total human genome sequence now provides opportunities to study its myriad gene products. However, the presence of alternative splicing, post-translational modifications, and innumerable protein-protein interactions among proteins occurring at widely different concentrations, all combine to place extreme demands on the specificity and sensitivity of assays. The choice of method also depends on matters such as whether proteins will be analyzed in body fluids and lysates, or localized inside single cells. In this review we discuss commonly used detection methods and compare these to the recently-developed proximity ligation technique.</p>","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"28 ","pages":"85-93"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-0-387-34504-8_5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26429932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-01-01DOI: 10.1007/978-0-387-34504-8_3
Zhipei Liu, Djeda Belharazem, Karl Rudolf Muehlbauer, Tatiana Nedelko, Yuri Knyazev, Monica Hollstein
{"title":"Mutagenesis of human p53 tumor suppressor gene sequences in embryonic fibroblasts of genetically-engineered mice.","authors":"Zhipei Liu, Djeda Belharazem, Karl Rudolf Muehlbauer, Tatiana Nedelko, Yuri Knyazev, Monica Hollstein","doi":"10.1007/978-0-387-34504-8_3","DOIUrl":"https://doi.org/10.1007/978-0-387-34504-8_3","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"28 ","pages":"45-54"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26429930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Anionic nutrient transport in plants: the molecular basis of the sulfate transporter gene family.","authors":"Hideki Takahashi, Naoko Yoshimoto, Kazuki Saito","doi":"10.1007/0-387-25856-6_5","DOIUrl":"https://doi.org/10.1007/0-387-25856-6_5","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"27 ","pages":"67-80"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/0-387-25856-6_5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25774370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marinus Pilon, Salah E Abdel-Ghany, Douglas Van Hoewyk, Hong Ye, Elizabeth A H Pilon-Smits
Iron-sulfur (Fe-S) clusters are co-factors of proteins that perform a number of biological roles, including electron transfer, redox and non-redox catalysis, regulation of gene expression, and as sensors within all living organisms, prokaryotes and eukaryotes. These clusters are thought to be among the oldest structures found in biological cells. In chloroplasts, Fe-S clusters play a key role in photosynthetic electron transport as well as nitrogen and sulfur assimilation. The capacity of the Fe atom in Fe-S clusters to take up an electron reversibly provides the required electron carrier capacity in these pathways. Iron and sulfur limitation both affect plant primary production and growth. It has long been known that iron deficiency leads to defects in photosynthesis and bleaching in young leaves, phenomena that are closely linked to a defect in chloroplastic photosystem-I (PSI) accumulation, a major Fe-S containing protein complex in plants. Although the functional importance of Fe-S cluster proteins is evident and isolated chloroplasts have been shown to be able to synthesize their own Fe-S clusters, much is yet to be learned about the biosynthesis of Fe-S proteins in plastids. The recent discovery of a NifS-like protein in plastids has hinted to the existence of an assembly machinery related to bacterial Fe-S assembly systems. This chapter aims to summarize what we presently know about the assembly of Fe-S clusters in plants with an emphasis on green plastids.
{"title":"Biogenesis of iron-sulfur cluster proteins in plastids.","authors":"Marinus Pilon, Salah E Abdel-Ghany, Douglas Van Hoewyk, Hong Ye, Elizabeth A H Pilon-Smits","doi":"10.1007/0-387-25856-6_7","DOIUrl":"https://doi.org/10.1007/0-387-25856-6_7","url":null,"abstract":"<p><p>Iron-sulfur (Fe-S) clusters are co-factors of proteins that perform a number of biological roles, including electron transfer, redox and non-redox catalysis, regulation of gene expression, and as sensors within all living organisms, prokaryotes and eukaryotes. These clusters are thought to be among the oldest structures found in biological cells. In chloroplasts, Fe-S clusters play a key role in photosynthetic electron transport as well as nitrogen and sulfur assimilation. The capacity of the Fe atom in Fe-S clusters to take up an electron reversibly provides the required electron carrier capacity in these pathways. Iron and sulfur limitation both affect plant primary production and growth. It has long been known that iron deficiency leads to defects in photosynthesis and bleaching in young leaves, phenomena that are closely linked to a defect in chloroplastic photosystem-I (PSI) accumulation, a major Fe-S containing protein complex in plants. Although the functional importance of Fe-S cluster proteins is evident and isolated chloroplasts have been shown to be able to synthesize their own Fe-S clusters, much is yet to be learned about the biosynthesis of Fe-S proteins in plastids. The recent discovery of a NifS-like protein in plastids has hinted to the existence of an assembly machinery related to bacterial Fe-S assembly systems. This chapter aims to summarize what we presently know about the assembly of Fe-S clusters in plants with an emphasis on green plastids.</p>","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"27 ","pages":"101-17"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/0-387-25856-6_7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25774372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phospholipid-derived signaling in plant response to temperature and water stresses.","authors":"Xuemin Wang","doi":"10.1007/0-387-25856-6_4","DOIUrl":"https://doi.org/10.1007/0-387-25856-6_4","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"27 ","pages":"57-66"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/0-387-25856-6_4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25774369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2006-01-01DOI: 10.1007/0-387-25856-6_11
Mark T Fisher
{"title":"Molecular roles of chaperones in assisted folding and assembly of proteins.","authors":"Mark T Fisher","doi":"10.1007/0-387-25856-6_11","DOIUrl":"https://doi.org/10.1007/0-387-25856-6_11","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"27 ","pages":"191-229"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25775470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification and analysis of microRNAs.","authors":"Shveta Bagga, Amy E Pasquinelli","doi":"10.1007/0-387-25856-6_1","DOIUrl":"https://doi.org/10.1007/0-387-25856-6_1","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"27 ","pages":"1-20"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/0-387-25856-6_1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25774366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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