American Journal of Plant Sciences最新文献

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Berberine Attenuated Aging-Accelerating Effect of High Temperature in Drosophila Model. 小檗碱在果蝇高温模型中的延缓衰老作用。

American Journal of Plant Sciences

Pub Date : 2014-02-01 DOI: 10.4236/ajps.2014.53037

Valeriya Navrotskaya, Gregory Oxenkrug, Lyudmila Vorobyova, Paul Summergrad

We have observed that berberine prolonged life span and improved viability of pupae and climbing activity of imagoes of wild-type Drosophila melanogaster maintained at 23°C. As a continuation of our studies of berberine effect on life span, we were interested to evaluate the effect of berberine of life span in flies maintained at a higher temperature (28°C) known to accelerate aging in wild type flies. Considering that genetically or pharmacologically induced deficiency of TRP conversion into KYN prolonged life span in a Drosophila model, we compared the effects of berberine, a powerful inhibitor of kynurenine (KYN) formation from tryptophan (TRP), on life span in wild type and in Drosophila melanogaster mutants (vermilion) with deficient TRP-KYN metabolism maintained at 23°C and 28°C. High (28°C) ambient temperature decreased life span in both wild type and vermilion flies. Aging accelerating effect of high temperature was more pronounced in Oregon than in vermilion flies (-60% vs. -40% decrease of mean life span, resp). Berberine attenuated the aging-accelerating effect of high temperature. Effect of berberine was more pronounced in Oregon (+46%) than in vermilion (+22%) flies. The obtained data suggested the possible involvement of TRP-KYN metabolism in the aging-acceleration effect of the high temperature and in protective effect of berberine.

我们观察到小檗碱延长了野生型黑腹果蝇在23℃环境下的寿命，提高了蛹活力和攀爬能力。作为我们对黄连素对寿命影响研究的延续，我们有兴趣评估黄连素对维持在已知加速野生型果蝇衰老的较高温度(28°C)下果蝇寿命的影响。考虑到遗传或药理学诱导的TRP转化为KYN的缺陷延长了果蝇模型的寿命，我们比较了小檗碱(一种强大的色氨酸(TRP)形成犬氨酸(KYN)的抑制剂)对野生型和在23°C和28°C下TRP-KYN代谢不足的果蝇突变体(朱红)的寿命的影响。高(28°C)环境温度降低了野生型和朱红蝇的寿命。高温的加速老化作用在俄勒冈州比在朱红蝇中更为明显(平均寿命分别减少60%和40%)。小檗碱能减弱高温的加速老化作用。小檗碱对俄勒冈州(+46%)的影响比对朱砂蝇(+22%)的影响更明显。结果表明，TRP-KYN的代谢可能参与了高温的加速衰老作用和小檗碱的保护作用。

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引用次数: 16

Berberine Prolongs Life Span and Stimulates Locomotor Activity of Drosophila melanogaster. 小檗碱延长果蝇寿命并刺激果蝇运动活动。

American Journal of Plant Sciences

Pub Date : 2012-07-01 DOI: 10.4236/ajps.2012.327123

V V Navrotskaya, G Oxenkrug, L I Vorobyova, P Summergrad

Drosophila melanogaster mutants with deficient kynurenine (KYN) formation from tryptophan (TRP) have longer life span than wild type flies. Administration of alpha-methyl-TRP and 5-methyl-TRY, the inhibitors of TRP-KYN metabolism, prolonged life span in wild-type flies. Both inhibitors are not available for human use. Berberine, an isoquinoline alkaloid isolated from Berberis aristata, is known as the herb widely used in traditional Chinese and Indian medicine. Berberin is a strong inhibitor of the enzyme catalyzing TRP conversion into KYN. Considering this particular feature we investigated the effect of berberine on life- and health-span in wild-type Drosophila melanogaster. The results of our study showed that Berberine extended mean, median and maximum life span of female flies. Berberine did not affect the number of pupae of filial generation and decreased their lethality. Berberine increased locomotor activity (vertical climbing). The results of the study suggest that berberine prolongs life- and improves health-span of Drosophila melanogaster. Berberine might be a candidate drug for prevention and treatment of aging and aging-associated medical and psychiatric disorders.

缺乏由色氨酸(TRP)形成的犬尿氨酸(KYN)的黑腹果蝇突变体比野生型果蝇寿命更长。α -甲基- trp和5-甲基- try (TRP-KYN代谢抑制剂)可延长野生型果蝇的寿命。这两种抑制剂都不能用于人类。小檗碱是一种从小檗中分离出来的异喹啉类生物碱，是中国和印度传统医学中广泛使用的草药。小檗碱是催化TRP转化为KYN的酶的强抑制剂。考虑到这一特点，我们研究了小檗碱对野生型黑腹果蝇寿命和健康期的影响。结果表明，小檗碱能延长雌蝇的平均寿命、中值寿命和最大寿命。小檗碱不影响子代蛹的数量，降低了它们的致死率。小檗碱增加运动活动(垂直攀爬)。研究结果表明，小檗碱可以延长果蝇的寿命，改善果蝇的健康状况。小檗碱可能是预防和治疗衰老及与衰老相关的医学和精神疾病的候选药物。

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引用次数: 0

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