Fatima Ferreira, Michael Wallner, Gabriele Gadermaier, Anja Erler, Gerhard Fritz, Otto Glatter, Martin Himly, Peter Briza, Ronald van Ree
{"title":"Physico-chemical characterization of candidate reference materials.","authors":"Fatima Ferreira, Michael Wallner, Gabriele Gadermaier, Anja Erler, Gerhard Fritz, Otto Glatter, Martin Himly, Peter Briza, Ronald van Ree","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77490,"journal":{"name":"Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M","volume":" 95","pages":"75-82; discussion 82-3, 100-4"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26233180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Summary of the response to the questionnaire marketing authorization of allergen products in Europe sent to national regulatory agencies.","authors":"Gerald Reese","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77490,"journal":{"name":"Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M","volume":" 95","pages":"43-4; discussion 44"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26233280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Although administration of protein allergens to subjects with allergic rhinitis and asthma is effective in modulating the immune response and reducing symptoms, alternative strategies to minimize the allergenicity of protein immunotherapy while improving the effectiveness are currently being investigated in animal models of asthma and in human subjects with allergic rhinitis. CpG DNA, and CpG DNA conjugated to a protein allergen have shown promise in animal models of asthma and have entered phase I/II clinical trials. Further, clinical trials are needed to determine whether any of these CpG DNA based therapies are safe and effective to use in subjects with allergic rhinitis and asthma.
{"title":"Prospects for CpG based immunotherapy in asthma and allergy.","authors":"David H Broide","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Although administration of protein allergens to subjects with allergic rhinitis and asthma is effective in modulating the immune response and reducing symptoms, alternative strategies to minimize the allergenicity of protein immunotherapy while improving the effectiveness are currently being investigated in animal models of asthma and in human subjects with allergic rhinitis. CpG DNA, and CpG DNA conjugated to a protein allergen have shown promise in animal models of asthma and have entered phase I/II clinical trials. Further, clinical trials are needed to determine whether any of these CpG DNA based therapies are safe and effective to use in subjects with allergic rhinitis and asthma.</p>","PeriodicalId":77490,"journal":{"name":"Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M","volume":" 95","pages":"217-20; discussion 220-2"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26631269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The approaches used in both basic research and clinical work in allergology must be reconsidered in the light of information technology. Internet resources are further amplified by the parallel development of other new tools, such as molecular biology and nanotechnology. Information technology, molecular biology and nanotechnology are tightly related entities that fully express their power once applied to biomedical fields. Bioinformatics applied to allergological research simplifies the approach to the increasing wealth of knowledge.
{"title":"Allergome: a unifying platform.","authors":"Adriano Mari, Enrico Scala","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The approaches used in both basic research and clinical work in allergology must be reconsidered in the light of information technology. Internet resources are further amplified by the parallel development of other new tools, such as molecular biology and nanotechnology. Information technology, molecular biology and nanotechnology are tightly related entities that fully express their power once applied to biomedical fields. Bioinformatics applied to allergological research simplifies the approach to the increasing wealth of knowledge.</p>","PeriodicalId":77490,"journal":{"name":"Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M","volume":" 95","pages":"29-39; discussion 39-40"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26233278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Quality and clinical efficacy of allergen mixtures.","authors":"Hans-Jørgen Malling","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77490,"journal":{"name":"Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M","volume":" 95","pages":"253-7; discussion 257"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26631728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The CREATE project: future perspectives.","authors":"Ronald van Ree","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77490,"journal":{"name":"Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M","volume":" 95","pages":"98-9; discussion 100-4"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26233183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Domingo Barber, Florentino Polo, Manuel Lombardero, Mayte Villalba, Rosalía Rodríguez
{"title":"The importance of minor allergens in allergen standardization.","authors":"Domingo Barber, Florentino Polo, Manuel Lombardero, Mayte Villalba, Rosalía Rodríguez","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77490,"journal":{"name":"Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M","volume":" 95","pages":"128-34; discussion 134, 155"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26233186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Helmut Fiebig, Helga Kahlert, Andreas Nandy, Martin Wald, Roland Suck, Bernhard Weber
{"title":"Test procedures for allergoids and hypoallergenic recombinant allergens: immunological characterization.","authors":"Helmut Fiebig, Helga Kahlert, Andreas Nandy, Martin Wald, Roland Suck, Bernhard Weber","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77490,"journal":{"name":"Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M","volume":" 95","pages":"135-46; discussion147-8, 155"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26233187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Usefulness of pollen chamber in the evaluation of clinical trials with allergen products.","authors":"Norbert Krug","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77490,"journal":{"name":"Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M","volume":" 95","pages":"167-70; discussion 170-1"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26233190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Renato Truffer, Michael B Stadler, Monique Vogel, Adriano Mari, Beda M Stadler
Background: We have recently reported a new method based on protein motifs to predict allergenicity by protein sequence. By using the more complete allergen database Allergome (www.allergome.org), as the basis for the motif identification process, we obtained a new set of allergenic motifs. We studied whether an allergenic motif may be used instead of a full length allergen for predicting allergenicity.
Methods: Sequence accession numbers of allergens were received from A. Mari of the Allergome Project. The sequences were downloaded and integrated in the allergen database, which was used to produce new motifs applicable for allergenicity prediction. The motif containing allergenic tropomyosins of different non vertebrate species was chosen. A consensus sequence of the tropomyosin motif was synthesized and expressed in Escherichia coli (E. coli). The purified peptide was analyzed for specificity with enzyme linked-immunosorbent assay (ELISA).
Results: Based on the Allergome dataset, we calculated 69 motifs containing 912 sequences. Prediction performance of this new set of allergenic motifs is increased compared to the original 52 motifs. The IgE immunological reactivity of the recombinant tropomyosin motif was comparable to that obtained with a full length recombinant shrimp tropomyosin (rPen a 1) tested in the ImmunoCAP system.
Discussion: The use of the motif-based allergenicity prediction method is more accurate than the guidelines proposed by FAO/WHO, however continuous updates of the motif dataset are necessary to guarantee a high prediction performance of this method. The experiments performed with the tropomyosin motif showed that peptides based on the consensus sequence of motifs may represent the allergenic epitope, which can be used in future diagnostic or therapeutic approaches.
{"title":"Computational resources: a regulatory need, a tool for research.","authors":"Renato Truffer, Michael B Stadler, Monique Vogel, Adriano Mari, Beda M Stadler","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>We have recently reported a new method based on protein motifs to predict allergenicity by protein sequence. By using the more complete allergen database Allergome (www.allergome.org), as the basis for the motif identification process, we obtained a new set of allergenic motifs. We studied whether an allergenic motif may be used instead of a full length allergen for predicting allergenicity.</p><p><strong>Methods: </strong>Sequence accession numbers of allergens were received from A. Mari of the Allergome Project. The sequences were downloaded and integrated in the allergen database, which was used to produce new motifs applicable for allergenicity prediction. The motif containing allergenic tropomyosins of different non vertebrate species was chosen. A consensus sequence of the tropomyosin motif was synthesized and expressed in Escherichia coli (E. coli). The purified peptide was analyzed for specificity with enzyme linked-immunosorbent assay (ELISA).</p><p><strong>Results: </strong>Based on the Allergome dataset, we calculated 69 motifs containing 912 sequences. Prediction performance of this new set of allergenic motifs is increased compared to the original 52 motifs. The IgE immunological reactivity of the recombinant tropomyosin motif was comparable to that obtained with a full length recombinant shrimp tropomyosin (rPen a 1) tested in the ImmunoCAP system.</p><p><strong>Discussion: </strong>The use of the motif-based allergenicity prediction method is more accurate than the guidelines proposed by FAO/WHO, however continuous updates of the motif dataset are necessary to guarantee a high prediction performance of this method. The experiments performed with the tropomyosin motif showed that peptides based on the consensus sequence of motifs may represent the allergenic epitope, which can be used in future diagnostic or therapeutic approaches.</p>","PeriodicalId":77490,"journal":{"name":"Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M","volume":" 95","pages":"11-5, discussion 15-6"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26233274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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