Somatosensory research最新文献

The retrograde transport of horseradish peroxidase (HRP) and immunocytochemistry for glutamic acid decarboxylase (GAD) have been employed to examine whether local circuit neurons (LCNs) exist in the dorsal column nuclei (DCN) and whether these neurons may be GABA-ergic. Observations focused on the dorsal part of the middle cuneate nucleus (MCd), since this region has been previously shown to contain projecting neurons whose axons terminate almost exclusively in the contralateral thalamus. After large injections of HRP in the nucleus ventralis posterolateralis and surrounding structures of the feline thalamus, the majority of neurons in MCd are labeled. These represent about 89% of the neurons in MCd as counted in 40-microns frozen sections, and about 69% as counted in plastic-embedded, 2.5-microns-thick section. Unlabeled by the same injections are some medium to large neurons at the dorsal rim of MCd, and many characteristically small (mean = +/- 250 microns2) neurons at the periphery of the cell clusters formed by thalamic-projecting neurons. These small neurons represent 10-12% of the neuronal population of MCd, as counted in 40-microns-thick frozen sections, and about 30%, as counted in plastic-embedded, 2.5-microns-thick sections. Neurons in this size range are also unlabeled after injection of retrograde tracer in the pretectal area, inferior and superior colliculi, inferior olivary complex, and/or spinal cord. These injections, however, result in the labeling of neurons along the dorsal rim of MCd and/or in other regions of the cuneate nucleus. In adult, colchicine-treated cats, the use of anti-GAD serum reveals a population of labeled neurons uniformly distributed throughout the DCN. In MCd, these are small (mean = +/- 235 microns2) neurons mainly intercalated between cell clusters, and represent about 25% of the neuronal population of this nuclear subdivision as counted in plastic-embedded, 2.5-microns-thick sections. Labeled processes densely infiltrate the cell clusters, and labeled varicosities appear to cover the soma and dendrites of unlabeled neurons. At the electron-microscopic level, most labeled profiles contain vesicles and correspond to F boutons usually involved in "axoaxonic" contacts with terminals of dorsal root afferent and presynaptic to dendrites. Other vesicle-containing, GAD-positive endings seem to correspond to the P boutons described by Ellis and Rustioni (1981) and are believed to be, at least in part, of dendritic origin. It is suggested that GAD-positive neurons are GABA-ergic LCNs and that these can mediate both pre- and postsynaptic inhibition.(ABSTRACT TRUNCATED AT 400 WORDS)

Some investigators have reported that deafferentation markedly alters the somatotopic organization of the dorsal horn in adult mammals, whereas others, including the present investigator, have not. Failure to observe changes in somatotopy might be attributable to insufficient deafferentation, inadequate sampling, limitation of the observations to a population of neurons that does not exhibit the phenomenon, or a variety of technical factors. The most striking changes in somatotopy have been reported following total deafferentation of the medial dorsal horn by sciatic and saphenous nerve lesions (Devor and Wall, 1981a; Lisney, 1983). The present study was an attempt to replicate this result to determine which explanations might account for the failure of some earlier investigations to observe changes in somatotopic organization. Other variables to be considered include type of anesthesia, type of electrode, and mapping and reconstruction procedures. The somatotopic organization of the lumbar dorsal horn in the adult cat was examined ipsilateral to sciatic and saphenous nerve lesions at postoperative survival times of 1-98 days. Half of these experiments were performed using chloralose anesthesia and half using pentobarbital. Microelectrodes were used to record single and multiunit activity in transverse rows of penetrations across the dorsal horn of L6 and adjacent segments. The results of these experiments were compared with data taken from dorsal horns ipsilateral to intact nerves. Sciatic and saphenous nerve lesions completely abolished dorsal horn neuronal responses to foot and toe stimulation at all survival times, yet there was no significant shift of proximal hindlimb representation into the medial dorsal horn following these lesions at any survival time. It is suggested that the method of anatomical reconstruction of recording sites used by investigators reporting such changes might best explain their findings.

The effects of skin indentation depth and rate on threshold and suprathreshold tactile sensations were investigated. Indentation rates between 0.3 and 10 mm/sec had little effect on the absolute tactile thresholds measured in terms of indentation depth. Slower rates resulted in increased absolute thresholds. Estimates of the growth in intensity of tactile sensations were made as functions of indentation depth and rate. The fastest rate used (10 mm/sec), for a given depth of indentation, produced the most intense sensation; the slowest (0.1 mm/sec), the least intense sensation. The tactile sensation magnitude estimates, with rate as the parameter, could be described by power functions. At the slowest indentation rate the exponent of the function was 1.36. At faster indentation rates (0.4, 1.0, and 10 mm/sec), two functions of markedly different slopes were required to describe the estimates. The exponents of the power functions were between 0.38 and 0.49 for indentation depths up to about 0.9 mm, and between 1.07 and 1.43 for deeper indentation depths.

The extent and nature of tonic supraspinal influences was determined on cat spinal cord dorsal horn neurons that received both noxious (radiant heat) and nonnoxious (hair movement) inputs or only a nonnoxious input. The former cells receive a tonic inhibition that descends in the dorsolateral funiculi and which is selective for the noxious input. The latter neurons are under a tonic facilitation.

An investigation was made of the capacities of humans to detect, by actively touching with the fingertip, the presence of a single, small asperity on a very smooth background. The asperity consisted of either a raised dot having a diameter of 602, 231, or 40 micron, or an edge, each etched into a silicon wafer using the methods of contact photolithography. The height of each dot or edge was varied and the subject was asked to make a forced choice on each test trial as to which of two wafers, one of which was blank, contained the asperity. The mean detection threshold, or minimal height of asperity corresponding to a d' of 1.35, was lowest for edges (0.85 +/- 0.22 micron, SD) and increased with decreases in the diameter of dot from 1.09 +/- 0.19 micron for a diameter of 602 micron to 2.94 +/- 1.19 micron and 5.97 +/- 2.02 micron for diameters of 231 micron and 40 micron, respectively. The type of skin displacement required for the detection of these small asperities was believed to be a local lateral deformation of the papillary ridges.

The responses of 302 neurons in the medial medullary reticular formation (MRF) to a variety of noxious and innocuous somatic stimuli were studied in anesthetized and awake rats. In addition, the effects of analgesic electrical stimulation in the mesencephalon (MES) on unit responses were examined. Tail shock was the most effective stimulus, exciting more than 80% of all units recorded. This stimulus was considered separately during data analysis, since it could not be classified as noxious or innocuous. Noxious somatic stimuli (including pinch, firm pressure, pin prick, and radiant heating of the tail above 45 degrees C were especially effective in eliciting discharge in a significant fraction of all cells in both awake (123/205) and anesthetized (45/97) animals. Nociceptive neurons could be classified as nociceptive specific (NS) or wide dynamic range (WDR) depending on their responses to all somatic stimuli tested. Nociceptive neurons showed no preferential anatomical distribution. Most neurons, including those responsive to noxious inputs, exhibited large, often bilateral receptive fields which frequently covered the tail, one or more limbs, and extensive areas of the body or head. Electrical stimulation within or adjacent to the mesencephalic periaqueductal gray matter depressed the spontaneous and evoked discharge of MRF neurons in both acute and chronic preparations. This inhibition showed a significant preference (p less than 0.001, chi-square statistic) for units that were excited by somatic and especially noxious stimuli. No units were facilitated by MES stimulation. In the awake rat, unit suppression closely followed the time course and level of MES-induced analgesia. Excitability data from the acute experiments suggest that this response inhibition may be the result of a direct action on MRF neurons. Anesthesia severely depressed the spontaneous discharge of MRF neurons as well as the activity evoked by innocuous somatic stimulation. Our data suggest that analgesia produced by MES stimulation is at least in part due to the depression of MRF unit activity, and support the hypothesis that MRF neurons play a critical role in the mediation of behavioral responses to noxious stimuli.

In order to study the synaptic relationships of serotonin (5-HT)-containing axons, boutons in laminae I and II of the cat spinal cord were labeled for serotonin with peroxidase-antiperoxidase immunocytochemistry. Labeled boutons were examined with the light microscope and recut into serial ultrathin sections for examination with the electron microscope. Labeled axons exhibiting boutons were sagittally oriented, and were most numerous in lamina I and outer lamina II (IIo) and least numerous in inner lamina II (IIi). Two types of labeled boutons were observed ultrastructurally. A relatively rare, large, scalloped or egg-shaped bouton, which contained many mitochondria and dense core vesicles, was found in laminae I and IIo. A smaller dome-shaped bouton, which contained fewer dense core vesicles and round or pleomorphic, clear vesicles, was found throughout laminae I and II. Both types commonly established symmetrical synaptic contacts with the distal portion of a dendritic tree, rarely with proximal portions or cell somas, and never with axon terminals. The results suggest that there are heterogeneous serotonergic systems that may selectively modify different inputs postsynaptically to functionally different types of neurons in the superficial dorsal horn of the spinal cord.