American journal of reproductive immunology : AJRI : official journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction最新文献
Pub Date : 2002-09-01DOI: 10.1034/j.1600-0897.2002.t01-2-00001.x
U. Hipler, G. Schreiber, W. Romer
{"title":"Stimulation and scavestrogen-induced inhibition of reactive oxygen species generated by rat sertoli cells","authors":"U. Hipler, G. Schreiber, W. Romer","doi":"10.1034/j.1600-0897.2002.t01-2-00001.x","DOIUrl":"https://doi.org/10.1034/j.1600-0897.2002.t01-2-00001.x","url":null,"abstract":"","PeriodicalId":79203,"journal":{"name":"American journal of reproductive immunology : AJRI : official journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction","volume":"48 1","pages":"153-153"},"PeriodicalIF":0.0,"publicationDate":"2002-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"57503208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-09-01DOI: 10.1034/J.1600-0897.2002.T01-2-00008.X
A. Iborra, J. R. Palacio, M. Mayorga, E. Garcia, Z. Ulčová-Gallová, P. Martínez
{"title":"Oxidative stress may develop endometrial autoimmune disorders","authors":"A. Iborra, J. R. Palacio, M. Mayorga, E. Garcia, Z. Ulčová-Gallová, P. Martínez","doi":"10.1034/J.1600-0897.2002.T01-2-00008.X","DOIUrl":"https://doi.org/10.1034/J.1600-0897.2002.T01-2-00008.X","url":null,"abstract":"","PeriodicalId":79203,"journal":{"name":"American journal of reproductive immunology : AJRI : official journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction","volume":"48 1","pages":"136-137"},"PeriodicalIF":0.0,"publicationDate":"2002-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"57503483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-09-01DOI: 10.1016/S0015-0282(02)04100-6
O. Yoshino, Y. Osuga, K. Koga, Yasushi Hirota, O. Tsutsumi, T. Yano, Y. Morita, M. Momoeda, T. Fujiwara, K. Kugu, Y. Taketani
{"title":"Concentrations of interferon-gamma-induced protein-10 (IP-10), an antiangiogenic substance, are decreased in peritoneal fluid of women with advanced endometriosis.","authors":"O. Yoshino, Y. Osuga, K. Koga, Yasushi Hirota, O. Tsutsumi, T. Yano, Y. Morita, M. Momoeda, T. Fujiwara, K. Kugu, Y. Taketani","doi":"10.1016/S0015-0282(02)04100-6","DOIUrl":"https://doi.org/10.1016/S0015-0282(02)04100-6","url":null,"abstract":"","PeriodicalId":79203,"journal":{"name":"American journal of reproductive immunology : AJRI : official journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction","volume":"50 1 1","pages":"60-5"},"PeriodicalIF":0.0,"publicationDate":"2002-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0015-0282(02)04100-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"55476342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-12-01DOI: 10.1034/j.1600-0897.2001.d01-27.x
S. Spandorfer, J. Navarro, D. Levy, A. R. Black, H.‐C. Liu, L. Veeck, S. Witkin, Z. Rosenwaks
PROBLEM: To determine if LIF produced by autologous endometrial co‐culture (ECC) was associated with outcome in 46 patients with a history of multiple IVF failures. METHOD OF STUDY: The conditioned media (CM) from ECC cells exposed or non‐exposed to human embryos was analyzed for LIF. RESULTS: Exposure or non‐exposure to an embryo did not result in differing levels of LIF in the CM. LIF levels were significantly greater in the CM than in the serum controls (LIF was not found in the serum controls). Embryos grown on ECC demonstrated a significant improvement in number of blastomeres and fragmentation when compared to embryos grown in conventional media without ECC (6.7±1.3 vs. 5.6±1.2 blastomeres and 17.6%±9.3 vs. 26.4%±9.8 fragmentation; P<0.05). When LIF levels were detectable in the CM, the embryos grown in ECC were of improved quality as compared to the embryos grown only in conventional media and demonstrated a non‐significant increase in pregnancy rates (60 vs. 48%, P=0.50). CONCLUSIONS: We have demonstrated a significant improvement in embryo quality with ECC. The cells in the ECC express LIF. The presence of LIF in the CM was associated with embryonic development and clinical pregnancy.
问题:确定46例有多次IVF失败史的患者中，自体子宫内膜共培养(ECC)产生的LIF是否与结果相关。 研究方法:对暴露于或未暴露于人类胚胎的ECC细胞的条件培养基(CM)进行LIF分析。 结果:暴露或未暴露于胚胎不会导致CM中不同水平的LIF。CM患者的LIF水平明显高于血清对照组(血清对照组未发现LIF)。与在常规培养基中生长的胚相比，在ECC上生长的胚在卵裂球数量和碎裂率方面均有显著提高(6.7±1.3 vs 5.6±1.2)，碎裂率分别为17.6%±9.3 vs 26.4%±9.8;P < 0.05)。当CM中检测到LIF水平时，与仅在常规培养基中生长的胚胎相比，ECC中生长的胚胎质量更好，并且妊娠率无显著增加(60%对48%，P=0.50)。 结论:我们已经证明ECC显著改善了胚胎质量。ECC中的细胞表达LIF。CM中LIF的存在与胚胎发育和临床妊娠有关。
{"title":"Autologous Endometrial Coculture in Patients with In Vitro‐Fertilization (IVF) Failure: Correlations of Outcome with Leukemia Inhibiting Factor (LIF) Production 1","authors":"S. Spandorfer, J. Navarro, D. Levy, A. R. Black, H.‐C. Liu, L. Veeck, S. Witkin, Z. Rosenwaks","doi":"10.1034/j.1600-0897.2001.d01-27.x","DOIUrl":"https://doi.org/10.1034/j.1600-0897.2001.d01-27.x","url":null,"abstract":"PROBLEM: To determine if LIF produced by autologous endometrial co‐culture (ECC) was associated with outcome in 46 patients with a history of multiple IVF failures.\u2028 METHOD OF STUDY: The conditioned media (CM) from ECC cells exposed or non‐exposed to human embryos was analyzed for LIF.\u2028 RESULTS: Exposure or non‐exposure to an embryo did not result in differing levels of LIF in the CM. LIF levels were significantly greater in the CM than in the serum controls (LIF was not found in the serum controls). Embryos grown on ECC demonstrated a significant improvement in number of blastomeres and fragmentation when compared to embryos grown in conventional media without ECC (6.7±1.3 vs. 5.6±1.2 blastomeres and 17.6%±9.3 vs. 26.4%±9.8 fragmentation; P<0.05). When LIF levels were detectable in the CM, the embryos grown in ECC were of improved quality as compared to the embryos grown only in conventional media and demonstrated a non‐significant increase in pregnancy rates (60 vs. 48%, P=0.50).\u2028 CONCLUSIONS: We have demonstrated a significant improvement in embryo quality with ECC. The cells in the ECC express LIF. The presence of LIF in the CM was associated with embryonic development and clinical pregnancy.","PeriodicalId":79203,"journal":{"name":"American journal of reproductive immunology : AJRI : official journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2001-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1034/j.1600-0897.2001.d01-27.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"57495784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-12-01DOI: 10.1016/S0002-9378(01)80578-0
G. Holcberg, O. Sapir, M. Hallak, A. Alaa, Haj-Yhia Shorok, Yohay David, M. Katz, M. Huleihel
{"title":"Selective vasodilator effect of magnesium sulfate in human placenta.","authors":"G. Holcberg, O. Sapir, M. Hallak, A. Alaa, Haj-Yhia Shorok, Yohay David, M. Katz, M. Huleihel","doi":"10.1016/S0002-9378(01)80578-0","DOIUrl":"https://doi.org/10.1016/S0002-9378(01)80578-0","url":null,"abstract":"","PeriodicalId":79203,"journal":{"name":"American journal of reproductive immunology : AJRI : official journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction","volume":"51 3 1","pages":"192-7"},"PeriodicalIF":0.0,"publicationDate":"2001-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0002-9378(01)80578-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"55511937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-12-01DOI: 10.1034/j.1600-0897.2001.d01-33.x
Timothy A. Sato, Deepak K. Gupta, J. Keelan, K. Marvin, M. Mitchell
PROBLEM: The pro‐inflammatory cytokine interleukin (IL)‐1β has been shown to stimulate the production of prostaglandins (PG) in gestational tissues. Increased PG synthesis is considered a key step in the initiation of labor both at term and preterm. In this study, IL‐1β mRNA in the uterus and gestational tissues of mice during mid to late pregnancy was studied to characterize its tissue specific as well as gestational age expression. METHOD OF STUDY: Gestational tissues (placenta, decidual cap and fetal membranes), uterus, and cervix were collected from pregnant mice during gestation. Total RNA was isolated and probed for the expression of IL‐1β mRNA. RESULTS: There was a significantly increased expression of IL‐1β mRNA in the uterus on day 18 of pregnancy. In the decidual caps, there was increased expression of IL‐1β mRNA on day 14 of pregnancy and a decrease in expression with the onset of labor. In the fetal membranes and placenta, IL‐1β mRNA expression significantly increased on days 14 and 18 of pregnancy, respectively, and then remained elevated for the duration of pregnancy. In the cervix, there was a decrease in expression with labor onset. CONCLUSIONS: The increases in IL‐1β mRNA in the fetal membranes and placenta late in pregnancy are consistent with a localized, tissue specific inflammatory activation involved in the initiation of parturition.
{"title":"Expression of Interleukin‐1β mRNA in Murine Uterine and Gestational Tissues: Relationship with Gestational Age 1","authors":"Timothy A. Sato, Deepak K. Gupta, J. Keelan, K. Marvin, M. Mitchell","doi":"10.1034/j.1600-0897.2001.d01-33.x","DOIUrl":"https://doi.org/10.1034/j.1600-0897.2001.d01-33.x","url":null,"abstract":"PROBLEM: The pro‐inflammatory cytokine interleukin (IL)‐1β has been shown to stimulate the production of prostaglandins (PG) in gestational tissues. Increased PG synthesis is considered a key step in the initiation of labor both at term and preterm. In this study, IL‐1β mRNA in the uterus and gestational tissues of mice during mid to late pregnancy was studied to characterize its tissue specific as well as gestational age expression.\u2028 METHOD OF STUDY: Gestational tissues (placenta, decidual cap and fetal membranes), uterus, and cervix were collected from pregnant mice during gestation. Total RNA was isolated and probed for the expression of IL‐1β mRNA.\u2028 RESULTS: There was a significantly increased expression of IL‐1β mRNA in the uterus on day 18 of pregnancy. In the decidual caps, there was increased expression of IL‐1β mRNA on day 14 of pregnancy and a decrease in expression with the onset of labor. In the fetal membranes and placenta, IL‐1β mRNA expression significantly increased on days 14 and 18 of pregnancy, respectively, and then remained elevated for the duration of pregnancy. In the cervix, there was a decrease in expression with labor onset.\u2028 CONCLUSIONS: The increases in IL‐1β mRNA in the fetal membranes and placenta late in pregnancy are consistent with a localized, tissue specific inflammatory activation involved in the initiation of parturition.","PeriodicalId":79203,"journal":{"name":"American journal of reproductive immunology : AJRI : official journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2001-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1034/j.1600-0897.2001.d01-33.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"57496614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-09-01DOI: 10.1034/j.1600-0897.2001.d01-2.x
V. R. Reddy, Sadhana M. Gupta, P. Meherji
PROBLEM: To investigate the expression of integrin (ITG) cell adhesion molecules on peripheral blood lymphocytes (PBL) and their correlation with endometrial cell ITG expression in fertile and infertile women during peak uterine receptive period (day 19/20). METHOD OF STUDY: Surface marker expression and quantification of α6, α4 and β3 ITG subunits was done by immunohistochemistry, indirect immunofluroscence and cell‐enzyme‐linked immunosorbent assay methods using endometrial cells and PBL obtained from fertile and infertile (unexplained infertility) women. RESULTS: The expression of ITGs was significantly (P<0.001) decreased in the endometrial cells of infertile women compared to normal fertile women. These results correlated well with the data obtained using PBL‐ITG expression. CONCLUSIONS: If these preliminary data are consistent in a larger group of patients, the expression of α4 and β3‐ITG subunits on PBL may be used as clinical markers to assess endometrial receptivity in infertile women. Moreover, frequent blood sampling is advantageous over repeated endometrial biopsies as the former approach is easier, non‐traumatic and avoids intra‐uterine infections.
{"title":"Expression of Integrin Receptors on Peripheral Lymphocytes: Correlation with Endometrial Receptivity 1","authors":"V. R. Reddy, Sadhana M. Gupta, P. Meherji","doi":"10.1034/j.1600-0897.2001.d01-2.x","DOIUrl":"https://doi.org/10.1034/j.1600-0897.2001.d01-2.x","url":null,"abstract":"PROBLEM: To investigate the expression of integrin (ITG) cell adhesion molecules on peripheral blood lymphocytes (PBL) and their correlation with endometrial cell ITG expression in fertile and infertile women during peak uterine receptive period (day 19/20).\u2028 METHOD OF STUDY: Surface marker expression and quantification of α6, α4 and β3 ITG subunits was done by immunohistochemistry, indirect immunofluroscence and cell‐enzyme‐linked immunosorbent assay methods using endometrial cells and PBL obtained from fertile and infertile (unexplained infertility) women.\u2028 RESULTS: The expression of ITGs was significantly (P<0.001) decreased in the endometrial cells of infertile women compared to normal fertile women. These results correlated well with the data obtained using PBL‐ITG expression.\u2028 CONCLUSIONS: If these preliminary data are consistent in a larger group of patients, the expression of α4 and β3‐ITG subunits on PBL may be used as clinical markers to assess endometrial receptivity in infertile women. Moreover, frequent blood sampling is advantageous over repeated endometrial biopsies as the former approach is easier, non‐traumatic and avoids intra‐uterine infections.","PeriodicalId":79203,"journal":{"name":"American journal of reproductive immunology : AJRI : official journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2001-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1034/j.1600-0897.2001.d01-2.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"57495354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Rockefeller Brothers Fund is currently accepting applications for its 2003 Charles E. Culpeper Scholarships in Medical Science Program designed to support the career development of academic physicians. Up to four awards of $100,000 per year for 3 years will be made to United States medical schools or equivalent United States educational institutions on behalf of candidates who are U.S. citizens or aliens who have been granted permanent U.S. residence (proof required); who have received their M.D. from a U.S. medical school or the equivalent of an M.D. from an educational institution equivalent to a United States Medical School in 1994 or later (except under extraordinary circumstances, as approved by the Fund before submittal); and who are judged worthy of support by virtue of the quality of their research proposals. All scientific research relevant to human health is eligible for consideration. No institution may nominate more than one candidate. In selecting awardees, emphasis will be on identifying young physicians with clear potential for making substantial contributions to science as academic physicians. Since January 1988, 49 physicians have been selected as Charles E. Culpeper Medical Scholars. Deadline for applications is August 15, 2002. Awards will be announced in January 2003, for activation on or about July 1, 2003. Application forms and instructions may be obtained on the Web at www.rbf.org or by contacting:
洛克菲勒兄弟基金会目前正在接受2003年Charles E. Culpeper医学科学奖学金的申请，该奖学金旨在支持学术医生的职业发展。将代表美国公民或获得美国永久居留权的外国人候选人(需要证明)，向美国医学院或同等的美国教育机构提供最多四项每年10万美元的奖励，为期三年;在1994年或之后获得美国医学院的医学博士学位或相当于美国医学院的教育机构的医学博士学位(特殊情况除外，在提交申请前得到基金批准);并且根据他们的研究建议的质量来判断他们是否值得支持。所有与人类健康有关的科学研究都有资格获得审议。任何院校不得提名多于一名候选人。在选择获奖者时，重点将放在确定作为学术医生对科学作出重大贡献的明显潜力的年轻医生上。自1988年1月以来，49名医生被选为Charles E. Culpeper医学学者。申请截止日期为2002年8月15日。奖项将于2003年1月公布，并于2003年7月1日左右启动。申请表格和说明可在www.rbf.org网站上获取，或联系:
{"title":"Charles E. Culpeper Scholarships in Medical Science","authors":"Charles E. Culpeper","doi":"10.1210/endo.143.5.9997","DOIUrl":"https://doi.org/10.1210/endo.143.5.9997","url":null,"abstract":"The Rockefeller Brothers Fund is currently accepting applications for its 2003 Charles E. Culpeper Scholarships in Medical Science Program designed to support the career development of academic physicians. Up to four awards of $100,000 per year for 3 years will be made to United States medical schools or equivalent United States educational institutions on behalf of candidates who are U.S. citizens or aliens who have been granted permanent U.S. residence (proof required); who have received their M.D. from a U.S. medical school or the equivalent of an M.D. from an educational institution equivalent to a United States Medical School in 1994 or later (except under extraordinary circumstances, as approved by the Fund before submittal); and who are judged worthy of support by virtue of the quality of their research proposals. All scientific research relevant to human health is eligible for consideration. No institution may nominate more than one candidate. In selecting awardees, emphasis will be on identifying young physicians with clear potential for making substantial contributions to science as academic physicians. Since January 1988, 49 physicians have been selected as Charles E. Culpeper Medical Scholars. Deadline for applications is August 15, 2002. Awards will be announced in January 2003, for activation on or about July 1, 2003. Application forms and instructions may be obtained on the Web at www.rbf.org or by contacting:","PeriodicalId":79203,"journal":{"name":"American journal of reproductive immunology : AJRI : official journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2001-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66005551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-05-01DOI: 10.1111/j.8755-8920.2001.450503.x
Calvin M. Johnson, P. C. Crawford, G. Papadi, S. Bortnick
PROBLEM: Human infants infected in utero with HIV develop thymus insufficiency and progress to AIDS sooner than infants infected peripartum. However, direct analysis of the thymus is difficult due to limited tissue access and variable timing of vertical transmission. METHOD OF STUDY: Fetal and neonatal cats were inoculated with feline immunodeficiency virus (FIV) at an equivalent infectious dose. The thymus, blood, and lymph nodes were harvested and compared at 23 and 46 days post‐inoculation (p.i.) and also compared to sham‐inoculated, age‐matched controls. Lymphocyte phenotypes were analyzed by flow cytometry and virus burden was quantified in histologic sections and by virus isolation from plasma. RESULTS: Fetal cats inoculated with FIV had acute thymus atrophy at birth, which coincided with peak viremia. At 46 days p.i., thymus size and cell composition rebounded and supported increased productive infection. In contrast, neonatal cats inoculated with FIV developed chronic thymus atrophy and degeneration, which was associated with decreasing productive infection and low‐level viremia. CONCLUSIONS: The fetal thymus is uniquely vulnerable to acute, transient depletion and high‐level productive infection. The neonatal thymus is less vulnerable to acute changes, and responds through progressive atrophy and declining productive infection. Reduced immune competence, as reflected by the failure to control virus replication, may contribute to the accelerated progression of FIV and HIV infections in utero.
{"title":"Unique Susceptibility of the Fetal Thymus to Feline Immunodeficiency Virus Infection: An Animal Model for HIV Infection In Utero 1","authors":"Calvin M. Johnson, P. C. Crawford, G. Papadi, S. Bortnick","doi":"10.1111/j.8755-8920.2001.450503.x","DOIUrl":"https://doi.org/10.1111/j.8755-8920.2001.450503.x","url":null,"abstract":"PROBLEM: Human infants infected in utero with HIV develop thymus insufficiency and progress to AIDS sooner than infants infected peripartum. However, direct analysis of the thymus is difficult due to limited tissue access and variable timing of vertical transmission.\u2028 METHOD OF STUDY: Fetal and neonatal cats were inoculated with feline immunodeficiency virus (FIV) at an equivalent infectious dose. The thymus, blood, and lymph nodes were harvested and compared at 23 and 46 days post‐inoculation (p.i.) and also compared to sham‐inoculated, age‐matched controls. Lymphocyte phenotypes were analyzed by flow cytometry and virus burden was quantified in histologic sections and by virus isolation from plasma.\u2028 RESULTS: Fetal cats inoculated with FIV had acute thymus atrophy at birth, which coincided with peak viremia. At 46 days p.i., thymus size and cell composition rebounded and supported increased productive infection. In contrast, neonatal cats inoculated with FIV developed chronic thymus atrophy and degeneration, which was associated with decreasing productive infection and low‐level viremia.\u2028 CONCLUSIONS: The fetal thymus is uniquely vulnerable to acute, transient depletion and high‐level productive infection. The neonatal thymus is less vulnerable to acute changes, and responds through progressive atrophy and declining productive infection. Reduced immune competence, as reflected by the failure to control virus replication, may contribute to the accelerated progression of FIV and HIV infections in utero.","PeriodicalId":79203,"journal":{"name":"American journal of reproductive immunology : AJRI : official journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2001-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.8755-8920.2001.450503.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63729118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.1111/%28ISSN%291600-0897
J. Harness, P. Mccombe
PROBLEM The present study was performed to explore the effects of pregnancy on experimental autoimmune encephalomyelitis (EAE) induced in Lewis rats by inoculation with myelin basic protein (MBP) (MBP-EAE). METHOD OF STUDY MBP-EAE was induced in pregnant and non-pregnant rats and severity of disease evaluated. Serum from pregnant and non-pregnant rats was used in standard lymphocyte proliferation assays. Real-time polymerase chain reaction (PCR) was used to investigate the expression of cytokine mRNA in the inflammatory cells obtained from the spinal cord of rats on day 15 after inoculation. RESULTS Pregnant rats developed less severe disease than non-pregnant rats. Serum from pregnant rats suppressed the proliferation of T lymphocytes in response to MBP. There was significantly increased expression of IL-4, IL-10 and TNF-alpha mRNA in the spinal cord infiltrate of pregnant rats. CONCLUSION Circulating humoral factors and alteration in cytokine production by inflammatory cells may contribute to the suppression of EAE in pregnant rats.
{"title":"The effects of pregnancy on myelin basic protein-induced experimental autoimmune encephalomyelitis in Lewis rats: suppression of clinical disease, modulation of cytokine expression in the spinal cord inflammatory infiltrate and suppression of lymphocyte proliferation by pregnancy sera.","authors":"J. Harness, P. Mccombe","doi":"10.1111/%28ISSN%291600-0897","DOIUrl":"https://doi.org/10.1111/%28ISSN%291600-0897","url":null,"abstract":"PROBLEM The present study was performed to explore the effects of pregnancy on experimental autoimmune encephalomyelitis (EAE) induced in Lewis rats by inoculation with myelin basic protein (MBP) (MBP-EAE). METHOD OF STUDY MBP-EAE was induced in pregnant and non-pregnant rats and severity of disease evaluated. Serum from pregnant and non-pregnant rats was used in standard lymphocyte proliferation assays. Real-time polymerase chain reaction (PCR) was used to investigate the expression of cytokine mRNA in the inflammatory cells obtained from the spinal cord of rats on day 15 after inoculation. RESULTS Pregnant rats developed less severe disease than non-pregnant rats. Serum from pregnant rats suppressed the proliferation of T lymphocytes in response to MBP. There was significantly increased expression of IL-4, IL-10 and TNF-alpha mRNA in the spinal cord infiltrate of pregnant rats. CONCLUSION Circulating humoral factors and alteration in cytokine production by inflammatory cells may contribute to the suppression of EAE in pregnant rats.","PeriodicalId":79203,"journal":{"name":"American journal of reproductive immunology : AJRI : official journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction","volume":"46 6 1","pages":"405-12"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"62971646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
American journal of reproductive immunology : AJRI : official journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction
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