G Zünd, A L Dzus, D K McGuirk, C Breuer, T Shinoka, J E Mayer, S P Colgan
Unlabelled: Hypoxemia is a common event in many vascular diseases, especially vascular ischemia. Since endothelial cells of blood vessels are exposed to conditions within the vascular space and leucocytes play a key role in ischemia/reperfusion injury, we hypothesized that endothelial exposure to hypoxia may regulate expression of surface proteins important in leucocyte-endothelial interactions, such as E-selectin and intercellular adhesion molecule (ICAM-1). In this study, we used isolated bovine aortic endothelial monolayers to examine endothelial surface alterations of E-selectin and ICAM-1 induced by tumor necrosis factor-alpha (TNF-alpha), lipopolysaccharide (LPS) and hypoxia using a whole cell enzyme-linked immunosorbent assay (ELISA). Bovine endothelial exposure to TNF-alpha (50 ng/mL) induced a time dependent increase (range 0-24h) in specific E-selection surface expression. Endothelial exposure to hypoxia alone (pO2 approximately 3 mmHg, range 0-24 h), however, failed to elicit endothelial E-selectin expression. Endothelial exposure to LPS brought about a dose- and time-dependent (range 0.5 ng/mL and 2-8 h) increase in specific ICAM-1 surface expression (max. 3.5 +/- 0.15-fold increase over no cytokine control at 10 ng/mL, 4 h). Hypoxia (pO2 approximately 3 mmHg, 8h), however, did not induce ICAM-1 surface expression over normoxia levels.
In conclusion: i) bovine endothelial E-selectin and ICAM-1 surface expression are regulated molecules, ii) hypoxia, per se, does not regulate surface expression of either E-selectin or ICAM-1. These results suggest that hypoxic endothelia may require additional external signals for generation of adaptive inflammatory responses.
{"title":"Hypoxic stress alone does not modulate endothelial surface expression of bovine E-selectin and intercellular adhesion molecule-1 (ICAM-1).","authors":"G Zünd, A L Dzus, D K McGuirk, C Breuer, T Shinoka, J E Mayer, S P Colgan","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Unlabelled: </strong>Hypoxemia is a common event in many vascular diseases, especially vascular ischemia. Since endothelial cells of blood vessels are exposed to conditions within the vascular space and leucocytes play a key role in ischemia/reperfusion injury, we hypothesized that endothelial exposure to hypoxia may regulate expression of surface proteins important in leucocyte-endothelial interactions, such as E-selectin and intercellular adhesion molecule (ICAM-1). In this study, we used isolated bovine aortic endothelial monolayers to examine endothelial surface alterations of E-selectin and ICAM-1 induced by tumor necrosis factor-alpha (TNF-alpha), lipopolysaccharide (LPS) and hypoxia using a whole cell enzyme-linked immunosorbent assay (ELISA). Bovine endothelial exposure to TNF-alpha (50 ng/mL) induced a time dependent increase (range 0-24h) in specific E-selection surface expression. Endothelial exposure to hypoxia alone (pO2 approximately 3 mmHg, range 0-24 h), however, failed to elicit endothelial E-selectin expression. Endothelial exposure to LPS brought about a dose- and time-dependent (range 0.5 ng/mL and 2-8 h) increase in specific ICAM-1 surface expression (max. 3.5 +/- 0.15-fold increase over no cytokine control at 10 ng/mL, 4 h). Hypoxia (pO2 approximately 3 mmHg, 8h), however, did not induce ICAM-1 surface expression over normoxia levels.</p><p><strong>In conclusion: </strong>i) bovine endothelial E-selectin and ICAM-1 surface expression are regulated molecules, ii) hypoxia, per se, does not regulate surface expression of either E-selectin or ICAM-1. These results suggest that hypoxic endothelia may require additional external signals for generation of adaptive inflammatory responses.</p>","PeriodicalId":79460,"journal":{"name":"Swiss surgery. Supplement","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19630432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study investigates the physical properties and the in vivo application of in vivo of a self-expandable stent (Wallstent) with an incorporated sealant of microporous polyurethane. Measurements of diameter and length as a function axial load featured the data for the calculation for radial pressure. Porosity control was assessed in comparison to non gelatin-sealed knitted Dacron. 11 stents on a 11.5F catheter were inserted in the aorta of three minipigs and evaluated by pressure measurement and angiography. The coverage of the stent causes an increase of 0.05-0.1 bar of radial pressure. The graph received by calculations demonstrates the corresponding pressure for every given diameter of the stents. Porosity is similar to the Dacron graft. The patency-rate is 100%. Characteristics of this covered stents meet requirements of an endovascular prostheses. Exclusion of fistulas and small aneurysms seems to be possible.
{"title":"[Self-expanding endoluminal vascular prosthesis: experimental basis and in-vivo evaluation].","authors":"B Marty, T Marty, L K von Segesser, M Turina","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The present study investigates the physical properties and the in vivo application of in vivo of a self-expandable stent (Wallstent) with an incorporated sealant of microporous polyurethane. Measurements of diameter and length as a function axial load featured the data for the calculation for radial pressure. Porosity control was assessed in comparison to non gelatin-sealed knitted Dacron. 11 stents on a 11.5F catheter were inserted in the aorta of three minipigs and evaluated by pressure measurement and angiography. The coverage of the stent causes an increase of 0.05-0.1 bar of radial pressure. The graph received by calculations demonstrates the corresponding pressure for every given diameter of the stents. Porosity is similar to the Dacron graft. The patency-rate is 100%. Characteristics of this covered stents meet requirements of an endovascular prostheses. Exclusion of fistulas and small aneurysms seems to be possible.</p>","PeriodicalId":79460,"journal":{"name":"Swiss surgery. Supplement","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19630431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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